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XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP
  • Sequence Map
    Editing disabled on read-only sequence.
    agtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaagctagctgctcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaataccgttcgatcgacgBmtIMfeINheISFFV20406080100120140160
    agtaacgccattattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggctcattgcggtaataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggcgtcaaagccggggccgEcoRVBstEIISFFV180200220240260280300320340
    ccgaggccaagagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttctgcttcccgagctctatggctccggttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagacgaagggctcgagataAflIIBbvCIBpu10ISFFV360380400420440460480500520
    aaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGAACTCCTTCTCCACAAGCGCCTTCGGTCCAGTTGCCTTCTCCCTGGGCCTGCTCCTGGTGTTGCCTGCTGCCTTCCCTGCCCCAGTCTTCACACTCGAAttttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACTTGAGGAAGAGGTGTTCGCGGAAGCCAGGTCAACGGAAGAGGGACCCGGACGAGGACCACAACGGACGACGGAAGGGACGGGGTCAGAAGTGTGAGCTTAjuIAloIAscIHindIIIMN2SF4ST6SA8FG10PV12AF14SL16GL18LL20VL22PA24AF26PA28PV30FT32LE34599-1195IL-6 signal sequenceSFFVhomologNluc540560580600620640660680700
    GATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGCTAAAGCAACCCCTGACCGCTGTCTGTCGGCCGATGTTGGACCTGGTTCAGGAACTTGTCCCTCCACACAGGTCAAACAAAGTCTTAGAGCCCCACAGGCATTGAGGCTAGGTTTCCTAACAGGACTCGCCACTTTTACCCGACTTCTAGCTGTAGGTACAGTAGTAGGGCATACBsaBIEcoNIBpu10IDF36VG38DW40RQ42TA44GY46NL48DQ50VL52EQ54GG56VS58SL60FQ62NL64GV66SV68TP70IQ72RI74VL76SG78EN80GL82KI84DI86HV88II90PY92599-1195Nluc720740760780800820840860
    AAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGATTCCAGACTCGCCGCTGGTTTACCCGGTCTAGCTTTTTTAAAAATTCCACCACATGGGACACCTACTAGTAGTGAAATTCCACTAGGACGTGATACCGTGTGACCATTAGCTGCCCCAATGCGGCTTGTACTAGCTGATAAAGCCTGCCGGCATACTTCCGTAGCGGCACAAGCTBsaBIEG94LS96GD98QM100GQ102IE104KI106FK108VV110YP112VD114DH116HF118KV120IL122HY124GT126LV128ID130GV132TP134NM136ID138YF140GR142PY144EG146IA148VF150D599-1195Nluc8809009209409609801,0001,0201,040
    CGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGCATTCTGGCGgacgcgttgGAAGCCAGACACAAACAGAAAGCCGTTTTTCTAGTGACATTGTCCCTGGGACACCTTGCCGTTGTTTTAATAGCTGCTCGCGGACTAGTTGGGGCTGCCGAGGGACGACAAGGCTCATTGGTAGTTGCCTCACTGGCCGACCGCCGACACGCTTGCGTAAGACCGCctgcgcaacCTTCGGTCTGTGTTTGTCTTTBsmIKflIMluIG152KK154IT156VT158GT160LW162NG164NK166II168DE170RL172IN174PD176GS178LL180FR182VT184IN186GV188TG190WR192LC194ER196IL198A599-1195D1A2L3E1A2R3H4K5Q6K71205-1303Nluc5' Stop... 2017)homolog1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCAAhdIBaeIKpnIBamHIAcc65IS1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A281316-1426G1T2G1S2I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331205-1303ADAR substrate5' StopGo (2A; Loughran 2017)3xMS2 SGR5' stem1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCATGCTGCTCCTGTTGCTGCTGCTGGGCCTGAGGCTACATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGTACGACGAGGACAACGACGACGACCCGGACTCCGATGTAgeIPfoISpeIBfuAIBspMIPaqCIBsu36IY29H30S31I32P33P34T35Q36T371316-1426ML2LL4LL6LL8GL10RL12Q1538-3055T1S2E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331439-1537T1G23' StopGo (2A; Loughran 2017)secretion signal peptideSEAPADAR substrate3' stemhomolog1,4201,4401,4601,4801,5001,5201,5401,560
    GCTCTCCCTGGGCATCATCCCAGTTGAGGAGGAGAACCCGGACTTCTGGAACCGCGAGGCAGCCGAGGCCCTGGGTGCCGCCAAGAAGCTGCAGCCTGCACAGACAGCCGCCAAGAACCTCATCATCTTCCTGGGCGATGGGATGGGGGTGTCTACGGTGACAGCTGCCAGGATTCGAGAGGGACCCGTAGTAGGGTCAACTCCTCCTCTTGGGCCTGAAGACCTTGGCGCTCCGTCGGCTCCGGGACCCACGGCGGTTCTTCGACGTCGGACGTGTCTGTCGGCGGTTCTTGGAGTAGTAGAAGGACCCGCTACCCTACCCCCACAGATGCCACTGTCGACGGTCCTAAL14SL16GI18IP20VE22EE24NP26DF28WN30RE32AA34EA36LG38AA40KK42LQ44PA46QT48AA50KN52LI54IF56LG58DG60MG62VS64TV66TA68AR70I1538-3055SEAPsec...de1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CTAAAAGGGCAGAAGAAGGACAAACTGGGGCCTGAGATACCCCTGGCCATGGACCGCTTCCCATATGTGGCTCTGTCCAAGACATACAATGTAGACAAACATGTGCCAGACAGTGGAGCCACAGCCACGGCCTACCTGTGCGGGGTCAAGGGCAACTTCCAGACCATTGGCTTGAGATTTTCCCGTCTTCTTCCTGTTTGACCCCGGACTCTATGGGGACCGGTACCTGGCGAAGGGTATACACCGAGACAGGTTCTGTATGTTACATCTGTTTGTACACGGTCTGTCACCTCGGTGTCGGTGCCGGATGGACACGCCCCAGTTCCCGTTGAAGGTCTGGTAACCGAACTMscINdeIBstXIPflMIL72KG74QK76KD78KL80GP82EI84PL86AM88DR90FP92YV94AL96SK98TY100NV102DK104HV106PD108SG110AT112AT114AY116LC118GV120KG122NF124QT126IG128L1538-3055SEAPhomolog1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GTGCAGCCGCCCGCTTTAACCAGTGCAACACGACACGCGGCAACGAGGTCATCTCCGTGATGAATCGGGCCAAGAAAGCAGGGAAGTCAGTGGGAGTGGTAACCACCACACGAGTGCAGCACGCCTCGCCAGCCGGCACCTACGCCCACACGGTGAACCGCAACTGGTACTCGGACACGTCGGCGGGCGAAATTGGTCACGTTGTGCTGTGCGCCGTTGCTCCAGTAGAGGCACTACTTAGCCCGGTTCTTTCGTCCCTTCAGTCACCCTCACCATTGGTGGTGTGCTCACGTCGTGCGGAGCGGTCGGCCGTGGATGCGGGTGTGCCACTTGGCGTTGACCATGAGCCTAleIBcgIBplIBplIBstEIIS130AA132AR134FN136QC138NT140TR142GN144EV146IS148VM150NR152AK154KA156GK158SV160GV162VT164TT166RV168QH170AS172PA174GT176YA178HT180VN182RN184WY186SD1881538-3055SEAP1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CGCCGACGTGCCTGCCTCGGCCCGCCAGGAGGGGTGCCAGGACATCGCTACGCAGCTCATCTCCAACATGGACATTGACGTGATCCTAGGTGGAGGCCGAAAATACATGTTTCCCATGGGAACCCCAGACCCTGAGTACCCAGATGACTACAGCCAAGGTGGGACCAGGCTGGACGCGGCTGCACGGACGGAGCCGGGCGGTCCTCCCCACGGTCCTGTAGCGATGCGTCGAGTAGAGGTTGTACCTGTAACTGCACTAGGATCCACCTCCGGCTTTTATGTACAAAGGGTACCCTTGGGGTCTGGGACTCATGGGTCTACTGATGTCGGTTCCACCCTGGTCCGACCTGAvrIIAD190VP192AS194AR196QE198GC200QD202IA204TQ206LI208SN210MD212ID214VI216LG218GG220RK222YM224FP226MG228TP230DP232EY234PD236DY238SQ240GG242TR244LD2461538-3055SEAP2,1202,1402,1602,1802,2002,2202,2402,260
    GGGAAGAATCTGGTGCAGGAATGGCTGGCGAAGCGCCAGGGTGCCCGGTATGTGTGGAACCGCACTGAGCTCATGCAGGCTTCCCTGGACCCGTCTGTGACCCATCTCATGGGTCTCTTTGAGCCTGGAGACATGAAATACGAGATCCACCGAGACTCCACACTGGACCCCTCCCCCCTTCTTAGACCACGTCCTTACCGACCGCTTCGCGGTCCCACGGGCCATACACACCTTGGCGTGACTCGAGTACGTCCGAAGGGACCTGGGCAGACACTGGGTAGAGTACCCAGAGAAACTCGGACCTCTGTACTTTATGCTCTAGGTGGCTCTGAGGTGTGACCTGGGGAGGGPflMIGK248NL250VQ252EW254LA256KR258QG260AR262YV264WN266RT268EL270MQ272AS274LD276PS278VT280HL282MG284LF286EP288GD290MK292YE294IH296RD298ST300LD302PS3041538-3055SEAP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TGATGGAGATGACAGAGGCTGCCCTGCGCCTGCTGAGCAGGAACCCCCGCGGCTTCTTCCTCTTCGTGGAGGGTGGTCGCATCGACCATGGTCATCATGAAAGCAGGGCTTACCGGGCACTGACTGAGACGATCATGTTCGACGACGCCATTGAGAGGGCGGGCCAGCTCACCAGACTACCTCTACTGTCTCCGACGGGACGCGGACGACTCGTCCTTGGGGGCGCCGAAGAAGGAGAAGCACCTCCCACCAGCGTAGCTGGTACCAGTAGTACTTTCGTCCCGAATGGCCCGTGACTGACTCTGCTAGTACAAGCTGCTGCGGTAACTCTCCCGCCCGGTCGAGTGGTCBlpIPshAISacIILM306EM308TE310AA312LR314LL316SR318NP320RG322FF324LF326VE328GG330RI332DH334GH336HE338SR340AY342RA344LT346ET348IM350FD352DA354IE356RA358GQ360LT362S1538-3055SEAP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CGAGGAGGACACGCTGAGCCTCGTCACTGCCGACCACTCCCACGTCTTCTCCTTCGGAGGCTACCCCCTGCGAGGGAGCTCCATCTTCGGGCTGGCCCCTGGCAAGGCCCGGGACAGGAAGGCCTACACGGTCCTCCTATACGGAAACGGTCCAGGCTATGTGCTCAAGGACGGCGCTCCTCCTGTGCGACTCGGAGCAGTGACGGCTGGTGAGGGTGCAGAAGAGGAAGCCTCCGATGGGGGACGCTCCCTCGAGGTAGAAGCCCGACCGGGGACCGTTCCGGGCCCTGTCCTTCCGGATGTGCCAGGAGGATATGCCTTTGCCAGGTCCGATACACGAGTTCCTGCCGBlpIStuIXcmIE364ED366TL368SL370VT372AD374HS376HV378FS380FG382GY384PL386RG388SS390IF392GL394AP396GK398AR400DR402KA404YT406VL408LY410GN412GP414GY416VL418KD420G1538-3055SEAP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GCTCGGCCGGATGTTACCGAGAGCGAGAGCGGGAGCCCCGAGTATCGGCAGCAGTCAGCAGTGCCCCTGGACGAAGAGACCCACGCAGGCGAGGACGTGGCGGTGTTCGCGCGCGGCCCGCAGGCGCACCTGGTTCACGGCGTGCAGGAGCAGACCTTCATAGCGCACGTCATGGCGAGCCGGCCTACAATGGCTCTCGCTCTCGCCCTCGGGGCTCATAGCCGTCGTCAGTCGTCACGGGGACCTGCTTCTCTGGGTGCGTCCGCTCCTGCACCGCCACAAGCGCGCGCCGGGCGTCCGCGTGGACCAAGTGCCGCACGTCCTCGTCTGGAAGTATCGCGTGCAGTACCMauBISexAIA422RP424DV426TE428SE430SG432SP434EY436RQ438QS440AV442PL444DE446ET448HA450GE452DV454AV456FA458RG460PQ462AH464LV466HG468VQ470EQ472TF474IA476HV478M1538-3055SEAP2,8202,8402,8602,8802,9002,9202,9402,960
    CCTTCGCCGCCTGCCTGGAGCCCTACACCGCCTGCGACCTGGCACCCCCCGCCGGCACCACCGACGCCGCGCACCCGGGTtaggccggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgGGAAGCGGCGGACGGACCTCGGGATGTGGCGGACGCTGGACCGTGGGGGGCGGCCGTGGTGGCTGCGGCGCGTGGGCCCAatccggcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgacApaIPmeIPspOMI*1A480FA482AC484LE486PY488TA490CD492LA494PP496AG498TT500DA502AH504PG5061538-3055SEAPc-myc epitopePolyhi...e tag2,9803,0003,0203,0403,0603,0803,1003,1203,140
    atcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgBGH polyadenylation sequence3,1603,1803,2003,2203,2403,2603,2803,3003,320
    agcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccClaISphIBglIIBspDIBGH polyadenylation sequenceBGH pA3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaagcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggtttKflI3,5203,5403,5603,5803,6003,6203,6403,660
    aaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggaggtttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctccBarIMscIXbaIBsaAIPshAIHygromycinFRT3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctacgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggatEcoRIHygromycin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggaacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccPvuIAsiSIBfuAIBspMIRsrIIBstAPIHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgagcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactNdeIBstAPIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    cggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgAjuISacIIHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtacatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagtcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaPfoIScaIBsaAIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgBsmIPsiIBstZ17ISV40 pA4,9204,9404,9604,9805,0005,0205,0405,060
    gtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtc5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagagacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttctSapIBspQI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    acatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctcccttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggapUC ori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccgcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    ttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctcaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagpUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaaacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ctcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcagagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagt*W286HK284IL282SA280GI278EA276IQ274RN272RE2706246-7106Amp (bla)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcAhdIBglIBsrDIDM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220LL218PG216AV214KD212A6246-7106Amp (bla)6,3206,3406,3606,3806,4006,4206,4406,460
    cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtaAclIFspIBsrDIE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162DL160RT158VH156DG154M6246-7106Amp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagcaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcBcgIPvuIScaIN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104YE102VL100DN98QS96Y6246-7106Amp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactaAclIH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44GV42RA40GL38QD366246-7106Amp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaactSspIEA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M6246-7106Amp (bla)bla promoter7,0207,0407,0607,0807,1007,1207,1407,160
    atgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagcctacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggBglIISgrDIbla promoter7,1807,2007,2207,2407,2607,2807,300
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7317 bp5000550060006500700050010001500SEAPsecretion signal peptidehomologopposite edited A1538-3055GRo003.3 (DNA)EKo438.4 (DNA)XZp040.2 (DNA)EKo387.3 (DNA)BGHR Genewiz (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)missing in pSLQ2349,missing in pSLQ2349,A->T from pSLQ2349,FlpInF1 (DNA),+9SFFV,G->A from pSLQ2349,EK_SFFV_F2 (DNA),BstEII,BbvCI,Bpu10I,AflIIhomolog,Kozak,IL-6 signal sequence,EKo271.2 (DNA),XZp071.1 (DNA),+5AjuI,EcoNIBpu10I,BsaBINluc,599-1195,BsaBIKflIhomolog,1196-1204,XZp071.2 (DNA),pNSK124 (DNA),+55' StopGo (2A; Loughran 2017),1205-1303,1304-1309,+75' stem,ADAR substrate,readthrough,+123' stem,bulged,bulge opposite the other A,+93' StopGo (2A; Loughran 2017),1439-1537,+5XZp040.3 (DNA)Bsu36IMscI,NdeIpUC oriAhdIBsrDIBglIFspI,AclI,BsrDIAmp (bla),6246-7106,PvuI,ScaIBcgI,AclIbla promoter,SspI,SgrDI,BglII

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    7317

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