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pET15b_Bst-LF
  • Sequence Map
    Editing disabled on read-only sequence.
    ttcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgaaagaacttctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactZraIAatIIAmpR promoterAmpR_promoter20406080100120140160
    taaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgaccSspIAmpicillinAmpRAmpRAmpR promoter180200220240260280300320340
    atctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagttagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcacaactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcaScaIXmnIHincIIAmpicillinAmpR360380400420440460480500520
    cacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttAmpicillinAmpR540560580600620640660680700
    ccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacgtcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggPstIAmpicillinAmpR720740760780800820840860
    ttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagAhdIBsaIAmpicillinAmpR8809009209409609801,0001,0201,040
    actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttcAmpicillinAmpR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    atcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcgori1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacgcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgMmeIori1,4201,4401,4601,4801,5001,5201,5401,560
    acagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttattgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaataMmeIori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    agtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataacctcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggDrdIPciIAflIIIori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgcccataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatataccacgtgagagtcatgttagacgagactacggSapIBspQIbom1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtccgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagAccIDrdIPfoIBsaAIBsmBIEsp3IPflFIBstZ17ITth111IbompGEX_3_primerROP2,1202,1402,1602,1802,2002,2202,2402,260
    atcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcacttagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgaXmnIROP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcctacggaggcacattccccctaaagacaagtacccccattactatggctactttgctctctcctacgagtgctatgcccaatgactactacttgtacgggccaatgaccttgcaacactcccatttgttgaccgccatacctacgccgccctggtctctttttagtgagtcccag2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcattacggtcgcgaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgtaggacgctacgtctaggccttgtattaccacgtcccgcgactgaaggcgcaaaggtctgaaatgctttgtgcctttggcttctggtaagtacaacaacgagtccagcgtctgcaaaacgt2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggccaggacccaacgctgcccgagatgcgccgcgtgcggctgctggagatggcggacgcgcgtcgtcagcgaagtgcaagcgagcgcatagccactaagtaagacgattggtcattccgttggggcggtcggatcggcccaggagttgctgtcctcgtgctagtacgcgtgggcaccggtcctgggttgcgacgggctctacgcggcgcacgccgacgacctctaccgcctgcgcAvaIMscIBsoBIFspAIPpuMIPpuMI2,8202,8402,8602,8802,9002,9202,9402,960
    atggatatgttctgccaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagtacctatacaagacggttcccaaccaaacgcgtaagtgtcaagaggcgttcttaactaaccgaggttaagaacctcaccacttaggcaatcgctccacggcggccgaaggtaagtccagctccaccgggccgaggtacgtggcgctgcgttgcgcccctccgtctgttccatatctet (576 - 1190)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccagtgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgccgccgcggatgttaggtacggttgggcaaggtacacgagcggctccgccgtatttagcggcactgctagtcgccaggtcactagcttcaatccgaccattctcggcgctcgctaggaacttcgacagggactaccagcagtagatggacggacctgtcgtaccggacgttgcgcBstAPItet (576 - 1190)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggccgtagggctacggcggccttcgctcttcttagtattaccccttccggtaggtcggagcgcagcgcttgcggtcgttctgcatcgggtcgcgcagccggcggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccctggtcactgcttccgaactcgctcccNruItet (576 - 1190)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgcgacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcgggtggccttcctPshAItet (576 - 1190)3,5203,5403,5603,5803,6003,6203,6403,660
    gctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataaccclacItet (576 - 1190)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcggcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcHpaIBsmBIEsp3IHincIIlacI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttatagggtgatggctctataggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaEcoRVBssHIIlacI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaaApaIApoIBstEIIPspOMIlacI4,2204,2404,2604,2804,3004,3204,3404,360
    gatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccctacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggBclIMluIAflIIIlacI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    atcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggclacI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggtgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaBstAPIlacIlacI promoter4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcggcgttccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgPfoISphIEcoNIlacI promoter4,9204,9404,9604,9805,0005,0205,0405,060
    gccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcactataggggcggctttgttcgcgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagctctagagctagggcgctttaattatgctgagtgatatccccBglIISgrAIpBRrevB...primerT7 promoter5,0805,1005,1205,1405,1605,1805,2005,2205,240
    aattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccaTATGACCGACGAAGGCGAAAAACCGCTGGCGGGTATGGACTTTGCGATCGttaacactcgcctattgttaaggggagatctttattaaaacaaattgaaattcttcctctatatggtacccgtcgtcggtagtagtagtagtagtgtcgtcgccggaccacggcgcgccgtcggtATACTGGCTGCTTCCGCTTTTTGGCGACCGCCCATACCTGAAACGCTAGCBarINcoINdeIXbaIAsiSIMG2SS4HH6HH8HH10SS12GL14VP16RG18SH20MT22DE24GE26KP28LA30GM32DF34AI365317-7146Bst Large Fragment ORFlac operator6xHisthrombin siteT7_tr..._RBS5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CTGATAGCGTTACCGACGAGATGCTGGCAGATAAAGCAGCGCTAGTAGTTGAAGTTGTGGGCGATAACTACCATCATGCGCCGATCGTGGGTATCGCTTTGGCGAACGAGCGCGGCCGTTTTTTCCTGCGCCCGGAAACCGCACTGGCGGACCCGAAGTTTCTGGCTTGGCTGGGGACTATCGCAATGGCTGCTCTACGACCGTCTATTTCGTCGCGATCATCAACTTCAACACCCGCTATTGATGGTAGTACGCGGCTAGCACCCATAGCGAAACCGCTTGCTCGCGCCGGCAAAAAAGGACGCGGGCCTTTGGCGTGACCGCCTGGGCTTCAAAGACCGAACCGACCCAD38SV40TD42EM44LA46DK48AA50LV52VE54VV56GD58NY60HH62AP64IV66GI68AL70AN72ER74GR76FF78LR80PE82TA84LA86DP88KF90LA92WL94G5317-7146Bst Large Fragment ORF5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CGACGAAACGAAAAAAAAGACTATGTTCGATTCTAAACGTGCAGCGGTGGCTCTCAAATGGAAAGGTATCGAATTGCGCGGTGTTGTGTTCGACCTGCTGCTGGCTGCGTACCTGCTCGATCCTGCTCAGGCTGCGGGTGACGTGGCAGCTGTAGCGAAAATGCACCAGTACGAAGCTGCTTTGCTTTTTTTTCTGATACAAGCTAAGATTTGCACGTCGCCACCGAGAGTTTACCTTTCCATAGCTTAACGCGCCACAACACAAGCTGGACGACGACCGACGCATGGACGAGCTAGGACGAGTCCGACGCCCACTGCACCGTCGACATCGCTTTTACGTGGTCATGCTTBmgBID96ET98KK100KT102MF104DS106KR108AA110VA112LK114WK116GI118EL120RG122VV124FD126LL128LA130AY132LL134DP136AQ138AA140GD142VA144AV146AK148MH150QY152E5317-7146Bst Large Fragment ORF5,6205,6405,6605,6805,7005,7205,7405,760
    GCCGTACGCTCTGATGAAGCGGTTTATGGTAAAGGTGCGAAACGTACCGTGCCAGACGAACCAACCCTGGCGGAACACCTGGCTCGCAAAGCAGCCGCTATCTGGGCCCTGGAGGAGCCTCTGATGGATGAACTTCGCCGCAACGAACAGGACCGTCTGCTCACAGAACTGGAACCGGCATGCGAGACTACTTCGCCAAATACCATTTCCACGCTTTGCATGGCACGGTCTGCTTGGTTGGGACCGCCTTGTGGACCGAGCGTTTCGTCGGCGATAGACCCGGGACCTCCTCGGAGACTACCTACTTGAAGCGGCGTTGCTTGTCCTGGCAGACGAGTGTCTTGACCTTGApaIBseRIBsiWIPspOMIA154VR156SD158EA160VY162GK164GA166KR168TV170PD172EP174TL176AE178HL180AR182KA184AA186IW188AL190EE192PL194MD196EL198RR200NE202QD204RL206LT208EL210E5317-7146Bst Large Fragment ORF5,7805,8005,8205,8405,8605,8805,9005,9205,940
    AGCCGTTGGCGGGTATTCTGGCAAACATGGAGTTCACTGGTGTAAAAGTGGACACTAAGCGCCTGGAACAAATGGGTGCCGAACTGACTGAGCAGCTTCAGGCAGTTGAACGCCGCATCTACGAACTGGCGGGTCAGGAGTTTAACATCAACTCTCCTAAACAGCTGGGCACCGTTCGGCAACCGCCCATAAGACCGTTTGTACCTCAAGTGACCACATTTTCACCTGTGATTCGCGGACCTTGTTTACCCACGGCTTGACTGACTCGTCGAAGTCCGTCAACTTGCGGCGTAGATGCTTGACCGCCCAGTCCTCAAATTGTAGTTGAGAGGATTTGTCGACCCGTGGCAQ212PL214AG216IL218AN220ME222FT224GV226KV228DT230KR232LE234QM236GA238EL240TE242QL244QA246VE248RR250IY252EL254AG256QE258FN260IN262SP264KQ266LG268TV2705317-7146Bst Large Fragment ORF5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GTTGTTCGACAAACTGCAACTGCCGGTGTTGAAGAAAACGAAAACCGGCTACAGCACCTCCGCCGATGTTCTGGAAAAACTGGCACCGCATCACGAAATCGTTGAACACATCCTGCACTACCGTCAGCTGGGTAAACTGCAATCTACCTACATCGAGGGCTTACTGAAAGTAGTGCAACAAGCTGTTTGACGTTGACGGCCACAACTTCTTTTGCTTTTGGCCGATGTCGTGGAGGCGGCTACAAGACCTTTTTGACCGTGGCGTAGTGCTTTAGCAACTTGTGTAGGACGTGATGGCAGTCGACCCATTTGACGTTAGATGGATGTAGCTCCCGAATGACTTTCATCACLF272DK274LQ276LP278VL280KK282TK284TG286YS288TS290AD292VL294EK296LA298PH300HE302IV304EH306IL308HY310RQ312LG314KL316QS318TY320IE322GL324LK326VV3285317-7146Bst Large Fragment ORF6,1406,1606,1806,2006,2206,2406,2606,2806,300
    CACCCGGTGACCGGTAAAGTGCACACTATGTTCAACCAGGCTCTGACGCAGACCGGCCGCCTGTCCTCCGTTGAACCGAACCTGCAAAACATCCCCATCCGTCTGGAAGAGGGCCGTAAGATCCGTCAGGCATTCGTGCCGTCTGAACCGGACTGGCTGATTTTCGCTGCTGATTGTGGGCCACTGGCCATTTCACGTGTGATACAAGTTGGTCCGAGACTGCGTCTGGCCGGCGGACAGGAGGCAACTTGGCTTGGACGTTTTGTAGGGGTAGGCAGACCTTCTCCCGGCATTCTAGGCAGTCCGTAAGCACGGCAGACTTGGCCTGACCGACTAAAAGCGACGACTAAAgeIBstEIIDraIIIHP330VT332GK334VH336TM338FN340QA342LT344QT346GR348LS350SV352EP354NL356QN358IP360IR362LE364EG366RK368IR370QA372FV374PS376EP378DW380LI382FA384AD3865317-7146Bst Large Fragment ORF6,3206,3406,3606,3806,4006,4206,4406,460
    ACTCTCAAATCGAACTGCGTGTTCTTGCTCACATTGCGGAGGACGATAACCTGATTGAAGCATTCCGTCGTGGTCTGGACATTCACACTAAAACGGCTATGGACATTTTCCATGTTTCCGAAGAAGATGTGACCGCTAATATGCGTCGTCAGGCTAAAGCGGTAAACTTTGGTATTGAGAGTTTAGCTTGACGCACAAGAACGAGTGTAACGCCTCCTGCTATTGGACTAACTTCGTAAGGCAGCACCAGACCTGTAAGTGTGATTTTGCCGATACCTGTAAAAGGTACAAAGGCTTCTTCTACACTGGCGATTATACGCAGCAGTCCGATTTCGCCATTTGAAACCATABaeIYS388QI390EL392RV394LA396HI398AE400DD402NL404IE406AF408RR410GL412DI414HT416KT418AM420DI422FH424VS426EE428DV430TA432NM434RR436QA438KA440VN442FG444I5317-7146Bst Large Fragment ORF6,4806,5006,5206,5406,5606,5806,6006,6206,640
    CGTATACGGTATCTCCGATTACGGCCTGGCACAGAACCTGAACATCACCCGCAAAGAAGCGGCTGAGTTTATCGAACGTTATTTCGCCTCGTTCCCCGGTGTTAAACAGTACATGGATAACATCGTTCAAGAAGCTAAACAGAAGGGTTATGTGACCACCCTGCTGCACCGTCGTGCATATGCCATAGAGGCTAATGCCGGACCGTGTCTTGGACTTGTAGTGGGCGTTTCTTCGCCGACTCAAATAGCTTGCAATAAAGCGGAGCAAGGGGCCACAATTTGTCATGTACCTATTGTAGCAAGTTCTTCGATTTGTCTTCCCAATACACTGGTGGGACGACGTGGCAGCAAccIBstZ17IV446YG448IS450DY452GL454AQ456NL458NI460TR462KE464AA466EF468IE470RY472FA474SF476PG478VK480QY482MD484NI486VQ488EA490KQ492KG494YV496TT498LL500HR502R5317-7146Bst Large Fragment ORF6,6606,6806,7006,7206,7406,7606,7806,8006,820
    CGTTACCTGCCGGACATCACCTCCCGTAACTTCAACGTGCGTTCTTTCGCCGAACGTACGGCTATGAATACCCCTATCCAGGGCAGCGCGGCGGACATCATCAAAAAAGCCATGATCGACCTGAGCGTGCGCCTGCGCGAAGAACGTTTGCAGGCTCGCCTGCTTCTGCAAGTGCGCAATGGACGGCCTGTAGTGGAGGGCATTGAAGTTGCACGCAAGAAAGCGGCTTGCATGCCGATACTTATGGGGATAGGTCCCGTCGCGCCGCCTGTAGTAGTTTTTTCGGTACTAGCTGGACTCGCACGCGGACGCGCTTCTTGCAAACGTCCGAGCGGACGAAGACGTTCACGBsiWIR504YL506PD508IT510SR512NF514NV516RS518FA520ER522TA524MN526TP528IQ530GS532AA534DI536IK538KA540MI542DL544SV546RL548RE550ER552LQ554AR556LL558LQ560V5317-7146Bst Large Fragment ORF6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ACGATGAGCTGATCCTAGAAGCTCCAAAAGAGGAAATTGAACGTTTGTGCCGTCTGGTTCCGGAAGTTATGGAACAGGCTGTCGCACTGCGTGTTCCGCTTAAAGTTGATTACCATTACGGTCCCACTTGGTACGACGCCAAGTGAGgatccggctgctaacaaagcccgaaaggTGCTACTCGACTAGGATCTTCGAGGTTTTCTCCTTTAACTTGCAAACACGGCAGACCAAGGCCTTCAATACCTTGTCCGACAGCGTGACGCACAAGGCGAATTTCAACTAATGGTAATGCCAGGGTGAACCATGCTGCGGTTCACTCctaggccgacgattgtttcgggctttccBamHIDraIIIH562DE564LI566LE568AP570KE572EI574ER576LC578RL580VP582EV584ME586QA588VA590LR592VP594LK596VD598YH600YG602PT604WY606DA608K*6105317-7146Bst Large Fragment ORFT7_terminator7,0207,0407,0607,0807,1007,1207,1407,160
    aagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgttcgactcaaccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttcctccttgatataggcctatagggcgttctccgggccgtcatggccgtattggttcggatacggatgtcgtaggtcccactgccacBlpIEcoRVT7_terminatorT7 terminatorT7_Ter...rimer7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ccgaggatgacgatgagcgcattgttagatttcatacacggtgcctgactgcgttagcaatttaactgtgataaactaccgcattaaagcttatcgatgataagctgtcaaacatgagaaggctcctactgctactcgcgtaacaatctaaagtatgtgccacggactgacgcaatcgttaaattgacactatttgatggcgtaatttcgaatagctactattcgacagtttgtactcttApoIClaIBspDIEcoRIHindIIItet promoter7,3607,3807,4007,4207,4407,460
    • Plasmid
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    Editing disabled on read-only sequence.
    7470 bp550060006500700050010001500oriAmpRT7 terminator5317-7146

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    7470