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LSM081 SFFV-mCherry-SGc(stem-1(3xMS2)2-stops)cSG-EGFP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    agaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttactcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatgBspHI*pUC ori20406080100120140160
    caatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataagttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttattBsaIBsrDIW286HK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228172-1032Amp (bla)180200220240260280300320340
    accagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgAclIBglIFspIBsrDIWG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170172-1032Amp (bla)360380400420440460480500520
    ttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcatPvuIEN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112172-1032Amp (bla)540560580600620640660680700
    agatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaAclIBcgIXmnILH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54K172-1032Amp (bla)720740760780800820840860
    taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaataatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttatSspIG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M172-1032Amp (bla)bla promoter8809009209409609801,0001,0201,040
    ttattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagacBglIIBspHISgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    atgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccMfeISFFV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttatggcaagctagctgcagtaacgccattattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcacaataccgttcgatcgacgtcattgcggtaataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtgBmtINheISmaIXmaIEcoRVTspMISFFV1,4201,4401,4601,4801,5001,5201,5401,560
    cgcagtttcggccccggcccgaggccaagagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttgcgtcaaagccggggccgggctccggttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaaAflIIBbvCIBpu10IBssHIISFFV1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACgacgaagggctcgagatattttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGAscIMscISacISacISmaIXmaITspMIBssHIIEco53kIEco53kIHindIIIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26N1845-2552mCherrySFFV1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGAStuIG28HE30FE32IE34GE36GE38GR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84D1845-2552mCherry1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACTGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGAloISbfIY86LK88LS90FP92EG94FK96WE98RV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT1441845-2552mCherry2,1202,1402,1602,1802,2002,2202,2402,260
    CATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCBbsIBsgIKasIKasINarINarISfoISfoIStuIBbvCIPflMIPluTIPluTIBpu10IMG146WE148AS150SE152RM154YP156ED158GA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK2021845-2552mCherry2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTMluIXcmIBsrGISgrAIAflIIID1A2L3E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L212562-2660LD204IT206SH208NE210DY212TI214VE216QY218ER220AE222GR224HS226TG228GM230DE232LY234KS2361845-2552mCherry5' StopGo (2A; Loughran 2017)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    AGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAAACCCAAACGaccggTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTTTGGGTTTGCtggccAgeIBaeIKpnIBamHIAcc65IG1T2G1S2S1V2*3V4*5V6G7G8I9V10T11Y12T13I14R15V16R17L18R19H20H21Q22G23L24S25G26T27A28S29A30Y31H32S33I34P35P36T37Q38T39Q40T412673-2795K22L23A24G25D26V27E28S29N30P31G32P332562-2660T1G2ADAR substrateek04503xMS2 SGRpotential primer5' StopGo (2A; Loughran 2017)potential primer5' stem3' stempotential primer2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tactagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACatgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCACAGAGTTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGPfoISpeITliIXhoIBfuAIBspMIPaqCIPspXIPaeR7IS1S2T1S2VS2KG4EE6LF8TG10VV12PI14LV16EL18DG20D2913-3626E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P332808-29063' StopGo (2A; Loughran 2017)from ek450GFPpote...merpo...r2,8202,8402,8602,8802,9002,9202,9402,960
    GTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAGTTCATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTCAAGTATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGBstXIV22NG24HK26FS28VS30GE32GE34GD36AT38YG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78K2913-3626GFP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    AGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGAAACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGAACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCBarISnaBIQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98FF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG1382913-3626GFP3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTAAACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGCTTGCGGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGASspIHK140LE142YN144YN146SH148NV150YI152MA154DK156QK158NG160IK162VN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP1962913-3626GFP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTCGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACTGTATAAGtaggccggagggcccgaacaaaaactcatctcagaagaggatctgaataCTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGACATATTCatccggcctcccgggcttgtttttgagtagagtcttctcctagacttatApaIPspOMIDN198HY200LS202TQ204SA206LS208KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228IT230LG232MD234EL236YK2382913-3626*1GFPc-myc epitope3,5203,5403,5603,5803,6003,6203,6403,660
    gcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacBclIPmeIBtgZIBGH polyadenylation sequencePolyhi...e tag3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggcagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgBbsIClaINspISphIBglIIBspDIBGH polyadenylation sequenceBGH pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccaKflINaeINgoMIV4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctggggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagacBarIMscIXbaIPshAIFRTHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    atcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgatagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ttccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcggaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgccPvuIAsiSIBfuAIBspMIEcoRIBstAPIDraIIIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaacaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttRsrIIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctactcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaAjuIBtgZIBstAPIDraIIIHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    tcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgSacIIHygromycin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    acaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccggtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccPfoIHygromycin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacBsmINaeIPsiINgoMIVSV40 pA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgBstZ17ISV40 pA5,6205,6405,6605,6805,7005,7205,7405,760
    gttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaagcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttcSapIBspQI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttggNspIPciIAflIIIpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccpUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    cctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagtpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6645 bp450050005500600050010001500HygromycinSFFVG->A from pSLQ2349Amp (bla)pUC ori172-1032EKo152.2 (DNA)EKo412.2 (DNA)EK_SFFV_F (DNA)EKo271.3 (DNA)EKo309.1 (DNA)EKo326.2 (DNA)Cit_1 (DNA)pNSK019 (DNA)EKo242.3 (DNA)EKo387.3 (DNA)EKo409.1 (DNA)BGHR Genewiz (DNA)EKo326.3 (DNA)EKo152.1 (DNA)oLSM-pUC_ori_R (DNA)pUCoriR (DNA)BspHIBsaI,BsrDI,BglIFspIAclI,BsrDIPvuIBcgIAclIXmnISspIbla promoter,BspHISgrDIBglIIFlpInF1 (DNA)FlpInF (DNA)EKo285.1 (DNA),EWo118.11 (DNA),+4missing in pSLQ2349,NheI,BmtImissing in pSLQ2349A->T from pSLQ2349EcoRVTspMI,XmaI,SmaIBbvCI,Bpu10I,AflIIBtgZIAjuISacIIHyg_F (DNA)PfoI,NgoMIV,NaeISV40PA_R (DNA),PsiISV40 pA,SV40pA-R Genewiz (DNA),BsmIBstZ17IM13R Genewiz (DNA)M13-48REV Genewiz (DNA)BspQI,SapIpUCori5F (DNA)AflIIIPciINspIoLSM-pUC_ori_F (DNA)

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    6645

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