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pAAV-hSyn-Coff/Fon-eYFP
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtgtgtctagactgcagagggcggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcacacagatctgacgtctcccgEagIMluINotISbfIXbaIAflIIIPspOMIL-ITRHuman\S...omoter20406080100120140160
    cctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccggacgcatactcacgttcacccaaaatcctggtcctactccgccccacccccacggatggactgctggctggggctgggtgacctgttcgtgggttgggggtaaggggtttaacgcgtaggggatagtctctccccctcccctttgtcctacgccgctccgcgcacgcgtgacggApaIFspAIHuman\Synapsin\I\promoter180200220240260280300320340
    agcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgctcgaagtcgtggcgcctgtcacggaagcgggggcggaccgccgcgcgcggtggcggcggagtcgtgacttccgcgcgactgcagtgagcggccagggggcgtttgaggggaagggccggtggaaccagcgcaggcgcggcggcggccgggtcggcctggcgtggtgcgctccgcgBbvCIRsrIISacIIHuman\Synapsin\I\promoter360380400420440460480500520
    gagataggggggcacgggcgcgaccatctgcgctgcggcgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgagaaggatccataacttcgtataggatactttatacgaagttatgtagctggagcaatttgaaactctatccccccgtgcccgcgctggtagacgcgacgccgcggccgctgagtcgcgacggagtcagacgccacccgtcgcctcctcagcacagcacggactctcgcgtcagctcttcctaggtattgaagcatatcctatgaaatatgcttcaatacatcgacctcgttaaactttAfeIKasIMreINarISfoIBamHIPluTISgrAIHuman\Synapsin\I\promoterlox2722540560580600620640660680700
    agattgttgtattacctctagctgcgcttaataacttcgtatagtataccttatacgaagttatactagtgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagtctaacaacataatggagatcgacgcgaattattgaagcatatcatatggaatatgcttcaatatgatcacggtggtaccactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtgttcaagtcgcacaggccgctcAccINcoISpeIBstZ17IYFP\exon\1loxN720740760780800820840860
    ggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaaggtgagtacaggagggctagcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagataggtgcaaagaataacttcgtaccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttccactcatgtcctcccgatcgtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatctatccacgtttcttattgaagcatBmtINheIYFP\exon\1lox\2722\RevCE\donorlo...v8809009209409609801,0001,0201,040
    taaggtatactatacgaagttatggtaccgaagttcctattcttcaaatagtataggaacttcaattgttattggtgcaaagaatcacttccttaagtctttatactgcccaggaagttcctattcttcaaaaggtataggaacttcggcgcgccttacttgtacagctcgtccaattccatatgatatgcttcaataccatggcttcaaggataagaagtttatcatatccttgaagttaacaataaccacgtttcttagtgaaggaattcagaaatatgacgggtccttcaaggataagaagttttccatatccttgaagccgcgcggaatgaacatgtcgagcaggtAccIAscIBaeIKpnIMfeIAflIIBsrGIAcc65IBstZ17IF3F5lox\N\ReveYFP\exon\21,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctacggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacgagtcccgcctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccccacaagacgaccatcaccagccgctcgacgtgcgaeYFP\exon\21,2401,2601,2801,3001,3201,3401,3601,3801,400
    gccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgcggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggaggaacttcagctacgggaagtcgagctacgccaagtggtcccacagceYFP\exon\21,4201,4401,4601,4801,5001,5201,5401,560
    ccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgaagcactgcaggccgtagccgaaggtggtcacgagggtgggggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcttcgtgacgtccggcatcggcttccaccagtgctcccacceYFP\exon\21,5801,6001,6201,6401,6601,6801,7001,7201,740
    gccagggcacgggcagctgtgggacgacatgacttaaccagttaaccatgttctctgaacatgagaagggctggccactctccagagctcgaagttcctatactatttgaagaataggaacttcttgccttaacccagaaattatcactgttattctttagaatggtgcaaagagcggtcccgtgcccgtcgacaccctgctgtactgaattggtcaattggtacaagagacttgtactcttcccgaccggtgagaggtctcgagcttcaaggatatgataaacttcttatccttgaagaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttctcHpaISacIHincIIEco53kICE\donor/acceptorF3\ReveYFP\exon\21,7601,7801,8001,8201,8401,8601,8801,9001,920
    aagttcctataccttttgaagaataggaacttcgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccttcaaggatatggaaaacttcttatccttgaagcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggClaIBspDIBstBIEcoRIEcoRVHindIIIWPREF5\Rev1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggPflMIWPRE2,1202,1402,1602,1802,2002,2202,2402,260
    cctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagWPRE2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctcggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggagAfeIBbsIBmrIBplIBplIClaIEarITliIXhoIBglIIBspDISacIIPaeR7IWPREhGH\polyA2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgcccBbsICspCIhGH\polyA2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatatcagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataBlpINsiISphIBbvCIhGH\polyA2,8202,8402,8602,8802,9002,9202,9402,960
    tggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactcccaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggAloIBsaIEagINotIPmlIBsaAIBstXIRsrIIBstEIIhGH\polyAR-ITR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgtKasINarISbfISfoIPluTIR-ITR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    accatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttatggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatf1\Origin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccAloIBsaAIDraIIIf1\Origin3,5203,5403,5603,5803,6003,6203,6403,660
    gctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacaccccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggPsiI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgatacggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactat3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgtattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcEarIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactaAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    gagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccatactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtatScaIAmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    accatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttPvuIAmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccAseIBsaIAmpR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaBmrIAmpR4,9204,9404,9604,9805,0005,0205,0405,060
    ttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgttpUC\ori5,0805,1005,1205,1405,1605,1805,2005,2205,240
    aaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgcttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgapUC\ori5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatAlwNIpUC\ori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtcpUC\ori5,6205,6405,6605,6805,7005,7205,7405,760
    gggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtcccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC\ori5,7805,8005,8205,840
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    5856 bp42504500475050005250550025050075010001250eYFP\exon\2C->GL-ITR,SbfI,EagI,NotI,AflIII,MluI,XbaI,PspOMI,ApaI,FspAIHuman\Synapsin\I\promoter,SacII,BbvCI,RsrII,KasI,NarI,SfoI,MreI,SgrAI,PluTIlox2722,AfeI,BamHIloxN,KOZAK,YFP\exon\1,AccI,BstZ17I,SpeI,NcoICE\donor,lox\2722\Rev,NheI,BmtIlox\N\Rev,F3,AccI,BstZ17I,Acc65I,KpnI,BaeI,MfeIAflIIF5AscIBsrGIScaIPvuIAmpRAseIBsaIBmrIAlwNIpUC\ori,AflIII,PciI

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    5856