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pCAGGS-SspB-mScarlet-I
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgatcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacagctagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattPvuISpeICAG promoterCAG enhancer20406080100120140160
    cgccaatagggactttccattgacgtcaatgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctgataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacCAG promoterCAG enhancer180200220240260280300320340
    gcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcgtcatgtagatgcataatcagtagcgataatggtacccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccgcgcgcggtccgccccBsaAISnaBICAG promoterCAG enhancer360380400420440460480500520
    cggggcgggggcgagggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctgccccgcccccgctcccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcaacggaagcggggcacggggcgaCAG promoter540560580600620640660680700
    ccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcggcgcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgagcaaagaaaagacaccgacgcactttcggaatttcccgaggccctcccgggaaacacgAfeIApaIPfoIPspOMI720740760780800820840860
    gggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcccccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgcacacgcgctcccctcgcgccggcccccgccacggggcgccacgcccccccgacgAfeIEagI8809009209409609801,0001,0201,040
    gaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgccgccagcccgacattggggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcacgccccgcaccgcgccccgagcggcacggcccgBlpI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccgggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggaaacagggMreINaeIPaqCISgrAIBstAPINgoMIV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgtttagacacgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgccccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagagggagaggtcggagccccgacaggcgcccccctgccgacNaeINgoMIV1,4201,4401,4601,4801,5001,5201,5401,560
    ccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagAATTCcaccatgAGCTCCCggaagccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatcgatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcgaggacccgttgcacgaccaataacacgacagagtagtaaaaccgtttcTTAAGgtggtacTCGAGGGSacIAlwNIEcoRIEco53kICAG1640cag1700-seq-fwd1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CGAAACGCCCTAAGCTGCTGCGTGAATATTACGATTGGCTGGTTGATAACAGCTTTACCCCATATCTGGTGGTGGATGCCACATACCTGGGCGTGAACGTGCCCGTGGAGTATGTGAAAGACGGTCAGATCGTGCTGAATCTGTCTGCAAGTGCGACCGGCAACCTGCAACTGACGCTTTGCGGGATTCGACGACGCACTTATAATGCTAACCGACCAACTATTGTCGAAATGGGGTATAGACCACCACCTACGGTGTATGGACCCGCACTTGCACGGGCACCTCATACACTTTCTGCCAGTCTAGCACGACTTAGACAGACGTTCACGCTGGCCGTTGGACGTTGACTGBstXIBpu10ISspB1,7601,7801,8001,8201,8401,8601,8801,9001,920
    AAATGATTTTATCCAGTTCAACGCCCGCTTTAAGGGCGTGTCTCGTGAACTGTATATCCCGATGGGTGCCGCTCTGGCCATTTACGCTCGCGAGAACGGCGATGGTGTGATGTTCGAACCAGAAGAAATCTATGACGAGCTGAATATTGGTgAATTCACCATGGTGAGCAAGGGCTTTACTAAAATAGGTCAAGTTGCGGGCGAAATTCCCGCACAGAGCACTTGACATATAGGGCTACCCACGGCGAGACCGGTAAATGCGAGCGCTCTTGCCGCTACCACACTACAAGCTTGGTCTTCTTTAGATACTGCTCGACTTATAACCAcTTAAGTGGTACCACTCGTTCCCGAleIAloIMscINruIBstBIEcoRIMV2SK4G2086-2819SspBmScarlet-I1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GAGGCAGTGATCAAGGAGTTCATGCGGTTCAAGGTGCACATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCTCCTGGGACATCCTGTCCCCTCCGTCACTAGTTCCTCAAGTACGCCAAGTTCCACGTGTACCTCCCGAGGTACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGAGGACCCTGTAGGACAGGGAhdIBclIBstEIIE6AV8IK10EF12MR14FK16VH18ME20GS22MN24GH26EF28EI30EG32EG34EG36RP38YE40GT42QT44AK46LK48VT50KG52GP54LP56FS58WD60IL62S2086-2819mScarlet-I2,1202,1402,1602,1802,2002,2202,2402,260
    CTCAGTTCATGTACGGCTCCAGGGCCTTCATCAAGCACCCCGCCGACATCCCCGACTACTATAAGCAGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGCCGTGACCGTGACCCAGGACACCTCCCTGGAGGACGGCACCCTGATCTACAAGAGTCAAGTACATGCCGAGGTCCCGGAAGTAGTTCGTGGGGCGGCTGTAGGGGCTGATGATATTCGTCAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCGGCACTGGCACTGGGTCCTGTGGAGGGACCTCCTGCCGTGGGACTAGATGTTP64QF66MY68GS70RA72FI74KH76PA78DI80PD82YY84KQ86SF88PE90GF92KW94ER96VM98NF100ED102GG104AV106TV108TQ110DT112SL114ED116GT118LI120YK1222086-2819mScarlet-I2,2802,3002,3202,3402,3602,3802,4002,4202,440
    GGTGAAGCTCCGCGGCACCAACTTCCCTCCTGACGGCCCCGTAATGCAGAAGAAGACAATGGGCTGGGAAGCGTCCACCGAGCGGTTGTACCCCGAGGACGGCGTGCTGAAGGGCGACATTAAGATGGCCCTGCGCCTGAAGGACGGAGGCCGCTACCTGGCGGACTTCAAGACCCCACTTCGAGGCGCCGTGGTTGAAGGGAGGACTGCCGGGGCATTACGTCTTCTTCTGTTACCCGACCCTTCGCAGGTGGCTCGCCAACATGGGGCTCCTGCCGCACGACTTCCCGCTGTAATTCTACCGGGACGCGGACTTCCTGCCTCCGGCGATGGACCGCCTGAAGTTCTGGBbsIBseYIVK124LR126GT128NF130PP132DG134PV136MQ138KK140TM142GW144EA146ST148ER150LY152PE154DG156VL158KG160DI162KM164AL166RL168KD170GG172RY174LA176DF178KT1802086-2819mScarlet-I2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ACCTACAAGGCCAAGAAGCCCGTGCAGATGCCCGGCGCCTACAACGTtGACCGCAAGTTGGACATCACCTCCCACAACGAGGACTACACCGTGGTGGAACAGTACGAACGCTCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGctcgagggaggcggccgcgTGGATGTTCCGGTTCTTCGGGCACGTCTACGGGCCGCGGATGTTGCAaCTGGCGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGCACCACCTTGTCATGCTTGCGAGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCgagctccctccgccggcgcAleIEagINotITliIXcmIXhoIBsrGIPspXISgrAIHincIIPaeR7ITY182KA184KK186PV188QM190PG192AY194NV196DR198KL200DI202TS204HN206ED208YT210VV212EQ214YE216RS218EG220RH222ST224GG226MD228EL230YK232LE234GG236GR2382086-2819mScarlet-IMCS2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gttctagataagtcgacggatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaacaagatctattcagctgcctagaaaaagggagacggtttttaatacccctgtagtacttcggggaactcgtagactgaagaccgattatttcctttaaataaaagtaacgttatcacacaaccttaaaaaacacagagagtgagccttcctgtataccctcccgtttagtaaattAccISalIXbaIHincIIGS240R*242VD2442086-2819caggs-seq-revCAGGS-R2MCS2,8202,8402,8602,8802,9002,9202,9402,960
    aacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgtttttgtagtcttactcataaaccaaatctcaaaccgttgtatacgggtatacgaccgacggtacttgtttccaaccgatatttctccagtagtcatatactttgtcgggggacgacaggtaaggaataaggtatcttttcggaactgaactccaatctaaaaaaaatataaaacaaaAjuIPflMIBstAPI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgcagcccaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaaacacaataaaaaaagaaattgtagggattttaaaaggaatgtacaaaatgatcggtctaaaaaggaggagaggactgatgagggtcagtatcgacagggagaagagaatacctctagggagctggacgtcgggttcgaaccgcattagtaccagtatcgacaaaggacacactttNspIPciIPstIAflIIIHindIIIM13-rev3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ttgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatctcaattagtcagcaaccatagaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgcctaggcgtagagttaatcagtcgttggtatcBamHIBsaBIlac promoterSV40 OriLacO3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgaBplIBplISfiIStuIAvrIISV40 Ori3,5203,5403,5603,5803,6003,6203,6403,660
    aacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcggccaacgcgcggggagaggcggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctaggcgacgtaattacttagccggttgcgcgcccctctccgccBsmIPsiIBamHISV40 Ori3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcaNspIPciISapIBspQIAflIII3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggattttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctaDrdIBciVIColE1 origin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    acctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagtggacaggcggaaagagggaagcccttcgcaccgcgaaagagttacgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattcBseYIColE1 origin4,2204,2404,2604,2804,3004,3204,3404,360
    acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgAlwNIColE1 origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttColE1 origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactaccctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgAhdIAmp_R4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagactatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctBsaIAvaIIAmp_R4,9204,9404,9604,9805,0005,0205,0405,060
    gtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgacattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctFspIAvaIIAmp_R5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttaBcgIPvuIScaIAmp_R5,2605,2805,3005,3205,3405,3605,3805,4005,420
    acgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactXmnIAmp_R5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtBciVIAmp_R5,6205,6405,6605,6805,7005,7205,7405,760
    catttccccgaaaagtgccacctggtaaaggggcttttcacggtggacSexAI5,7805,790
    • Plasmid
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    Editing disabled on read-only sequence.
    5799 bp42504500475050005250550025050075010001250Amp_RCAG promoterCAG enhancer

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    5799