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Design a CRISPR guide

Assemble and transform a sequence

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tutorial: phcB - Rs GMI1000
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agtgcaattgggcaacgccggccagccaggcccggcactgccgcccgccttctccgatttcctccgacgtatgcaacaggacaacgccctgccggtctatttccacccgaccctcaccgtggtggtcgacgacagccagtcctttgtcgaaagcctcgggttccagatggacccgtcacgttaacccgttgcggccggtcggtccgggccgtgacggcgggcggaagaggctaaaggaggctgcatacgttgtcctgttgcgggacggccagataaaggtgggctgggagtggcaccaccagctgctgtcggtcaggaaacagctttcggagcccaaggtctacctgggcBtsIMfeISfiIBtsαIRSc273420406080100120140160
tcccgcgcgatgctggccttcaacgatccggaagaggcgctggcatggctgcgccagtggcactcgctacgcatgcccggcttcctgccggtgcgcgtcacccatgacgacctgaccttctccaccgagcgccgcaccatccagctcgacgtcgaccgcgtctaccgccagatccagggcgcgctacgaccggaagttgctaggccttctccgcgaccgtaccgacgcggtcaccgtgagcgatgcgtacgggccgaaggacggccacgcgcagtgggtactgctggactggaagaggtggctcgcggcgtggtaggtcgagctgcagctggcgcagatggcggtctaggZraIAatIIRSc2734180200220240260280300320340
acgaggtcaaccgtttcctgcagccgtccgtgatcgtggtggactacagcatgccgcgcatgaacggcctcgagttctgcgccaagctcaaggacctgccctgcatgacgatcctgctgaccggcatggccgacgagaacatcgccgtgcagggcttcaacgacggcctgatcgatgctccagttggcaaaggacgtcggcaggcactagcaccacctgatgtcgtacggcgcgtacttgccggagctcaagacgcggttcgagttcctggacgggacgtactgctaggacgactggccgtaccggctgctcttgtagcggcacgtcccgaagttgctgccggactagctTliIXhoIPspXIPaeR7IRSc2734360380400420440460480500520
ccgctacatcaagaaggatcatccggccatggccgagcgcctgagcacggagatcgaagcgctgcagatgcgctacttcagcaacctgtcgagcacgctgcgcgaactgctgtcgcgccattcgttcagcttcctctccgatccggcggtcaccaagctcgtgcgggagctggccggcgatgtagttcttcctagtaggccggtaccggctcgcggactcgtgcctctagcttcgcgacgtctacgcgatgaagtcgttggacagctcgtgcgacgcgcttgacgacagcgcggtaagcaagtcgaaggagaggctaggccgccagtggttcgagcacgccctcgaccggNcoIBstEIIRSc2734540560580600620640660680700
gcgcgctatcgcttcatcgagtactacctgtacccgcacccggccggcgtgctgatgctgaccgccgacggccgcgccaccctgatggtgatcgagaccggcgcgagcatgctcacgcacctggaggatgccgaggcctacgatgcccccgaggcgctgctcaagggcctgcgcgcgcgcgatagcgaagtagctcatgatggacatgggcgtgggccggccgcacgactacgactggcggctgccggcgcggtgggactaccactagctctggccgcgctcgtacgagtgcgtggacctcctacggctccggatgctacgggggctccgcgacgagttcccggacgcgcScaIRSc2734phcB Upstream720740760780800820840860
aaaagcagatcgtcccgttcttctggccgggagacggcatgtacacccacgcctgcgtcgaatgggagcagtactgcctgcccgccgaggtctgccaggggcgcgaaccgtactactacgcattgttcgacatgccgtcgcatctgctgccggcgcagatctacagctacacccattttcgtctagcagggcaagaagaccggccctctgccgtacatgtgggtgcggacgcagcttaccctcgtcatgacggacgggcggctccagacggtccccgcgcttggcatgatgatgcgtaacaagctgtacggcagcgtagacgacggccgcgtctagatgtcgatgtgggtScaIBglIIRSc2734phcB Upstream8809009209409609801,0001,0201,040
atttcttgccaactaccggcaggatccggacgcgctgaccggccgcaaaccgcgctgatgcggcccgccgattccttttgcagcgcgttgctccacgttgtgtggaccctgttcggcaagtacaatcgattgcgtcatccgatgaccatgacgtcagagcgtgggacgaatgatgtaaagaacggttgatggccgtcctaggcctgcgcgactggccggcgtttggcgcgactacgccgggcggctaaggaaaacgtcgcgcaacgaggtgcaacacacctgggacaagccgttcatgttagctaacgcagtaggctactggtactgcagtctcgcaccctgcttactacZraIAatIIBamHIDraIIIphcB UpstreamRSc27341,0601,0801,1001,1201,1401,1601,1801,2001,220
gccatgatgcctgcggcatcgtggcgaacgagggcatcgtcaagatacggcgccccggggttgttactgtgcgggattctccttgagagacgatttgggttgcgacagcggggcgcgcgcggaataggcgtgcgccatggatatcaggggagtgcatcgctccagtctgtttgcgcggtactacggacgccgtagcaccgcttgctcccgtagcagttctatgccgcggggccccaacaatgacacgccctaagaggaactctctgctaaacccaacgctgtcgccccgcgcgcgccttatccgcacgcggtacctatagtcccctcacgtagcgaggtcagacaaacgcNcoIEcoRVphcB Upstream1,2401,2601,2801,3001,3201,3401,3601,3801,400
gtcacgcgacgcccttgaccacgctgtcggtccggtaacggtggacgccgtctgccgtggctccgaccgggcctctcctccaatcatctcgaagcgggttcgggcgcgaggcgcccaggccgtcgcgcgccgcgcgcggccggctaacgccggacacgcttttacgactgatgcacagtgcgctgcgggaactggtgcgacagccaggccattgccacctgcggcagacggcaccgaggctggcccggagaggaggttagtagagcttcgcccaagcccgcgctccgcgggtccggcagcgcgcggcgcgcgccggccgattgcggcctgtgcgaaaatgctgactacgtBseRIphcB Upstream1,4201,4401,4601,4801,5001,5201,5401,560
gcggcatcccctgcccgacgggcagcaggccgccagaccgggaccgcggtgcggttccgggtgcatggtagtggagtgagcttcccgctggatggacgtttctcctgaagtctccggcaaattcgcaccgtgtactctcccaaccaaatcgatccggcagtcagcttccgcaactcgccgtaggggacgggctgcccgtcgtccggcggtctggccctggcgccacgccaaggcccacgtaccatcacctcactcgaagggcgacctacctgcaaagaggacttcagaggccgtttaagcgtggcacatgagagggttggtttagctaggccgtcagtcgaaggcgttgaVY2SP4NQ6ID8PA10VS12FR14N1705-3108phcB UpstreamphcB gene1,5801,6001,6201,6401,6601,6801,7001,7201,740
cccagggtcagcaggttcgcggcaccatcatcacgctgcagcggcgcgccctggtgatggaggtctacaacccctattccatcgtgcaggtcagcgaggtcctctccgacctggccatcaagatgggcacgcgccaggcctacatcggcaaggcggtggtggtcagcctggtcaagggtcccagtcgtccaagcgccgtggtagtagtgcgacgtcgccgcgcgggaccactacctccagatgttggggataaggtagcacgtccagtcgctccaggagaggctggaccggtagttctacccgtgcgcggtccggatgtagccgttccgccaccaccagtcggaccagttAscIBplIBplIPasIS16QG18QQ20VR22GT24II26TL28QR30RA32LV34ME36VY38NP40YS42IV44QV46SE48VL50SD52LA54IK56MG58TR60QA62YI64GK66AV68VV70SL72VN741705-3108phcB gene1,7601,7801,8001,8201,8401,8601,8801,9001,920
caccggcctcaccgccgtggtgtccgtcaccctgacggaagaatggcgcgggctggccgatgtccaggacagccccaaactggtcggcgacgaagcccgggcgttcgtccaggactgggaagagcggttccgcatccggcacaactaccagatcgtcgtcaatgaaatgcgggcggtggccggagtggcggcaccacaggcagtgggactgccttcttaccgcgcccgaccggctacaggtcctgtcggggtttgaccagccgctgcttcgggcccgcaagcaggtcctgacccttctcgccaaggcgtaggccgtgttgatggtctagcagcagttactttacgcccgcAloISrfITG76LT78AV80VS82VT84LT86EE88WR90GL92AD94VQ96DS98PK100LV102GD104EA106RA108FV110QD112WE114ER116FR118IR120HN122YQ124IV126VN128EM130RA1321705-3108phcB gene1,9401,9601,9802,0002,0202,0402,0602,0802,100
ttcctggccgaagtgtcgcgctgggtggaacaggtcgacctgtccgaatcgctgcccaaggacggccacggcaaattgcgcatggacgtgttccaggagctggccgagccgatcatgtacaagatcaagcactacctcgactggctcgaatcggaggctgccgaggtggagccggaaggaccggcttcacagcgcgacccaccttgtccagctggacaggcttagcgacgggttcctgccggtgccgtttaacgcgtacctgcacaaggtcctcgaccggctcggctagtacatgttctagttcgtgatggagctgaccgagcttagcctccgacggctccacctcggccPflMIFL134AE136VS138RW140VE142QV144DL146SE148SL150PK152DG154HG156KL158RM160DV162FQ164EL166AE168PI170MY172KI174KH176YL178DW180LE182SE184AA186EV188EP1901705-3108phcB gene2,1202,1402,1602,1802,2002,2202,2402,260
agctcgcgccgtcccaccggacattcgcccagacggcgttgcacccgctgctgctgcgcgcgcccttcgtctatcgcacctacaccaagccgctcggatacgccggcgattacgagatggtcaaccagatcctctccgatccccgcgaagggccgagcacctacttccagatcgttcgagcgcggcagggtggcctgtaagcgggtctgccgcaacgtgggcgacgacgacgcgcgcgggaagcagatagcgtggatgtggttcggcgagcctatgcggccgctaatgctctaccagttggtctaggagaggctaggggcgcttcccggctcgtggatgaaggtctagcaSacIEco53kIEL192AP194SH196RT198FA200QT202AL204HP206LL208LR210AP212FV214YR216TY218TK220PL222GY224AG226DY228EM230VN232QI234LS236DP238RE240GP242ST244YF246QI248V1705-3108phcB gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
caacgccaccttcctgagcgccgcggtagcgcgcgcgcaccgcaaccgcatcgatatcctggtcaactatctcggcgagcttgcggcccgtgcgcaggcggccgggcgccggttcaaggtgctgaacgtcggatgcggcccggccgcggaaatccagcgcttcatccgcgaacacgttgcggtggaaggactcgcggcgccatcgcgcgcgcgtggcgttggcgtagctataggaccagttgatagagccgctcgaacgccgggcacgcgtccgccggcccgcggccaagttccacgacttgcagcctacgccgggccggcgcctttaggtcgcgaagtaggcgcttgtgEcoRVN250AT252FL254SA256AV258AR260AH262RN264RI266DI268LV270NY272LG274EL276AA278RA280QA282AG284RR286FK288VL290NV292GC294GP296AA298EI300QR302FI304RE306H1705-3108phcB gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
cccgagccggaccggctcgcgttccggctcgtcgacttcagcgaggaaaccctcgaatacacgcgcaaggagatggatggcgtacagcacgcggccaagaaacaggtggacatcgagttcgtgcacgaatccgtccaccagttgctcaagcggcgtgtggcgccgggcacgcccggggctcggcctggccgagcgcaaggccgagcagctgaagtcgctcctttgggagcttatgtgcgcgttcctctacctaccgcatgtcgtgcgccggttctttgtccacctgtagctcaagcacgtgcttaggcaggtggtcaacgagttcgccgcacaccgcggcccgtgcgggcAloIP308EP310DR312LA314FR316LV318DF320SE322ET324LE326YT328RK330EM332DG334VQ336HA338AK340KQ342VD344IE346FV348HE350SV352HQ354LL356KR358RV360AP362GT364P1705-3108phcB gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
aggcgggcgaattcgatgcggtgtactgcgccggcctgttcgactatctgtccgacaaggtgtgcagccggttgctggcctacttcgccgccagcacacgccagggcggcaccgtgctcgcgaccaacgtgcacagcaacaacccggagaagctgggcatggagcacctgctggatccgcccgcttaagctacgccacatgacgcggccggacaagctgatagacaggctgttccacacgtcggccaacgaccggatgaagcggcggtcgtgtgcggtcccgccgtggcacgagcgctggttgcacgtgtcgttgttgggcctcttcgacccgtacctcgtggacgacctNruIE366AG368EF370DA372VY374CA376GL378FD380YL382SD384KV386CS388RL390LA392YF394AA396ST398RQ400GG402TV404LA406TN408VH410SN412NP414EK416LG418ME420HL422LE4241705-3108phcB gene2,8202,8402,8602,8802,9002,9202,9402,960
gtggtatctggtctaccgggacgaagagcgcatgacgaccctgctgccgccgggctcggagcaggtccgcgtcttcaccgacgataccggtgtcaacgtgttcgcccacgccgacgtggcagccatcgcctgagagcgcacgcaccatgatcgccgccaactaccagggctatcacaccatagaccagatggccctgcttctcgcgtactgctgggacgacggcggcccgagcctcgtccaggcgcagaagtggctgctatggccacagttgcacaagcgggtgcggctgcaccgtcggtagcggactctcgcgtgcgtggtactagcggcggttgatggtcccgatagtAgeIBaeIPaqCIMI2AA4NY6QG8YH103122-4450MI2AA4NY6QG8YH103122-4450WY426LV428YR430DE432ER434MT436TL438LP440PG442SE444QV446RV448FT450DD452TG454VN456VF458AH460AD462VA464AI466A*4681705-3108phcB genephcB DownstreamphcS gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
ccaggagctgtccgatctgcggctggccttcagccgcagcggagccgtcacgggcgtcgtgctggtgatgctcggcgtggggctcgactacggcctgtatccgcagcatcaggtgctgttcggcgtcgcgcggttcctcgtctcgctgatgatgctgaccgtgatgctcggcctcggtcctcgacaggctagacgccgaccggaagtcggcgtcgcctcggcagtgcccgcagcacgaccactacgagccgcaccccgagctgatgccggacataggcgtcgtagtccacgacaagccgcagcgcgccaaggagcagagcgactactacgactggcactacgagccggagQE12LS14DL16RL18AF20SR22SG24AV26TG28VV30LV32ML34GV36GL38DY40GL42YP44QH46QV48LF50GV52AR54FL56VS58LM60ML62TV64ML66GL683122-4450QE12LS14DL16RL18AF20SR22SG24AV26TG28VV30LV32ML34GV36GL38DY40GL42YP44QH46QV48LF50GV52AR54FL56VS58LM60ML62TV64ML66GL683122-4450phcB DownstreamphcS gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
cccacgcgatggggccggccgcgcgtgcaggcgctgacgctgctgtggctcgtgctgccgcagctcatgatcgtctggatgatctggtacaccgatggcgccgaatcgctgtattacgcggggctcaacctggcgatcttcgcggtcggcatcgtcatgccgctggggttcctgcgggtgcgctaccccggccggcgcgcacgtccgcgactgcgacgacaccgagcacgacggcgtcgagtactagcagacctactagaccatgtggctaccgcggcttagcgacataatgcgccccgagttggaccgctagaagcgccagccgtagcagtacggcgaccccaaggacgFseIBspHIPflMIPT70RW72GR74PR76VQ78AL80TL82LW84LV86LP88QL90MI92VW94MI96WY98TD100GA102ES104LY106YA108GL110NL112AI114FA116VG118IV120MP122LG124FL1263122-4450PT70RW72GR74PR76VQ78AL80TL82LW84LV86LP88QL90MI92VW94MI96WY98TD100GA102ES104LY106YA108GL110NL112AI114FA116VG118IV120MP122LG124FL1263122-4450phcB DownstreamphcS gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
agacggtgctgttcggggccttcacgtacatcatctgggtcatcgcctgcgtgatgcacgccggcggcatccaatcgcgcggcacgttcatcgtgcactcgctgttcatcctgttttcggtcgtggccagcgcggtctacacctacttcaacgaactgggccgattccagctctttctgccacgacaagccccggaagtgcatgtagtagacccagtagcggacgcactacgtgcggccgccgtaggttagcgcgccgtgcaagtagcacgtgagcgacaagtaggacaaaagccagcaccggtcgcgccagatgtggatgaagttgcttgacccggctaaggtcgagaaBsaAIQT128VL130FG132AF134TY136II138WV140IA142CV144MH146AG148GI150QS152RG154TF156IV158HS160LF162IL164FS166VV168AS170AV172YT174YF176NE178LG180RF182QL184F3122-4450QT128VL130FG132AF134TY136II138WV140IA142CV144MH146AG148GI150QS152RG154TF156IV158HS160LF162IL164FS166VV168AS170AV172YT174YF176NE178LG180RF182QL184F3122-4450phcB DownstreamphcS gene3,5203,5403,5603,5803,6003,6203,6403,660
ccagctgaaagaggaactggccgaaaagaacgagcagctggaagccatcaaccacagcctcaccgagatcaaggggcaattgctgcagcaggagaagatggtcgccatcggcaccctgtcggccggcctgctgcacgagatcaacaaccccgtcaacttctgcctgatggcgatcggtcgactttctccttgaccggcttttcttgctcgtcgaccttcggtagttggtgtcggagtggctctagttccccgttaacgacgtcgtcctcttctaccagcggtagccgtgggacagccggccggacgacgtgctctagttgttggggcagttgaagacggactaccgctagFseIMfeIPvuIAsiSIPvuIIPvuIIQ186LK188EE190LA192EK194NE196QL198EA200IN202HS204LT206EI208KG210QL212LQ214QE216KM218VA220IG222TL224SA226GL228LH230EI232NN234PV236NF238CL240MA242I3122-4450Q186LK188EE190LA192EK194NE196QL198EA200IN202HS204LT206EI208KG210QL212LQ214QE216KM218VA220IG222TL224SA226GL228LH230EI232NN234PV236NF238CL240MA242I3122-4450phcB DownstreamphcS gene3,6803,7003,7203,7403,7603,7803,8003,8203,840
gcggtggcgcaggaagacagcaccgccaaggccagcccgatgctgctcgaatgcctggccgacgccaaggaaggcatgcagcgcgtccagcacatcgtctccgacctgaagaccttcgcctaccgcagccccgagaagggcgtggccgacacgcccttcctggcggaaaaggccacgccaccgcgtccttctgtcgtggcggttccggtcgggctacgacgagcttacggaccggctgcggttccttccgtacgtcgcgcaggtcgtgtagcagaggctggacttctggaagcggatggcgtcggggctcttcccgcaccggctgtgcgggaaggaccgccttttccggtBsmIXmnIBsaXIA244VA246QE248DS250TA252KA254SP256ML258LE260CL262AD264AK266EG268MQ270RV272QH274IV276SD278LK280TF282AY284RS286PE288KG290VA292DT294PF296LA298EK300A3122-4450A244VA246QE248DS250TA252KA254SP256ML258LE260CL262AD264AK266EG268MQ270RV272QH274IV276SD278LK280TF282AY284RS286PE288KG290VA292DT294PF296LA298EK300A3122-4450phcB DownstreamphcS gene3,8603,8803,9003,9203,9403,9603,9804,0004,020
tcgattcggccatccgcctgaccagccacgagctgcgcggcgtggaagtaaaccgccagttgccgcccgacacgctggtgcgcggcgacgaagccgccatcatcggcgtgctgatcaacctgctgtccaatgccgcgctggcgatgcacaaggcgcagcgcgagcacccgtgcatagctaagccggtaggcggactggtcggtgctcgacgcgccgcaccttcatttggcggtcaacggcgggctgtgcgaccacgcgccgctgcttcggcggtagtagccgcacgactagttggacgacaggttacggcgcgaccgctacgtgttccgcgtcgcgctcgtgggcacgtaBclII302DS304AI306RL308TS310HE312LR314GV316EV318NR320QL322PP324DT326LV328RG330DE332AA334II336GV338LI340NL342LS344NA346AL348AM350HK352AQ354RE356HP358CI3603122-4450I302DS304AI306RL308TS310HE312LR314GV316EV318NR320QL322PP324DT326LV328RG330DE332AA334II336GV338LI340NL342LS344NA346AL348AM350HK352AQ354RE356HP358CI3603122-4450phcS gene4,0404,0604,0804,1004,1204,1404,1604,1804,200
cgacatctcggcggagtggcacaacggccggctgtacgtggtcgtcaaggacaacgggcccggcatcgcggagaagaacctgtcgcgcgtgttcgagccgttcttcaccacgcgcgaagtcggcaagggcctgggactgggcctgtcgatcagctacagcgtgatccagcaccacgctgtagagccgcctcaccgtgttgccggccgacatgcaccagcagttcctgttgcccgggccgtagcgcctcttcttggacagcgcgcacaagctcggcaagaagtggtgcgcgcttcagccgttcccggaccctgacccggacagctagtcgatgtcgcactaggtcgtggtgApaIBsaAIPspOMIDI362SA364EW366HN368GR370LY372VV374VK376DN378GP380GI382AE384KN386LS388RV390FE392PF394FT396TR398EV400GK402GL404GL406GL408SI410SY412SV414IQ416HH4183122-4450DI362SA364EW366HN368GR370LY372VV374VK376DN378GP380GI382AE384KN386LS388RV390FE392PF394FT396TR398EV400GK402GL404GL406GL408SI410SY412SV414IQ416HH4183122-4450phcS gene4,2204,2404,2604,2804,3004,3204,3404,360
ggcgggacactctcggtggccagcctggaaggcgaatgggcacgcttcaccttcgatctgccgcgcgcggaataggagcgagcatggacgccacgcccgacaccggcccgaccatcctgtatgtggacgacgagcagcaggcctgcaagtggttcacgcgcatggtctcggccagccgccctgtgagagccaccggtcggaccttccgcttacccgtgcgaagtggaagctagacggcgcgcgccttatcctcgctcgtacctgcggtgcgggctgtggccgggctggtaggacatacacctgctgctcgtcgtccggacgttcaccaagtgcgcgtaccagagccggtcMD2AT4PD6TG8PT10IL12YV14DD16EQ18QA20CK22WF24TR26MV28SA30S4459-5583GG420TL422SV424AS426LE428GE430WA432RF434TF436DL438PR440AE442*3122-4450GG420TL422SV424AS426LE428GE430WA432RF434TF436DL438PR440AE442*3122-4450phcR genephcS gene4,3804,4004,4204,4404,4604,4804,5004,5204,540
ctacaacgtgctgacggccaacagcgtggaagaagccaaggcggtgctgcgcgacgcgcacgaccgcatcggcgtggtgctgaccgacttccgcatgcccggcggcgacggcaccgagctgctgcgcttcatcgacgcggaatacagcgacatcgccgccatcctcgtcaccgcggatgttgcacgactgccggttgtcgcaccttcttcggttccgccacgacgcgctgcgcgtgctggcgtagccgcaccacgactggctgaaggcgtacgggccgccgctgccgtggctcgacgacgcgaagtagctgcgccttatgtcgctgtagcggcggtaggagcagtggcgcBsiWIY32NV34LT36AN38SV40EE42AK44AV46LR48DA50HD52RI54GV56VL58TD60FR62MP64GG66DG68TE70LL72RF74ID76AE78YS80DI82AA84IL86VT88A4459-5583phcR gene4,5604,5804,6004,6204,6404,6604,6804,7004,720
tacgccgacaaggacctgctgatccaggccgtcaacaccgggcgggtcttcaagatcctggagaagccctaccagccggaggacgtgcggcgctcgctgcaggaggcgctggcactgcgccaggaccgcctgctgcgcacgcagcgcttgatggccatcgacgagacgctggcgtatgcggctgttcctggacgactaggtccggcagttgtggcccgcccagaagttctaggacctcttcgggatggtcggcctcctgcacgccgcgagcgacgtcctccgcgaccgtgacgcggtcctggcggacgacgcgtgcgtcgcgaactaccggtagctgctctgcgaccgcaBtsIBtsαIY90AD92KD94LL96IQ98AV100NT102GR104VF106KI108LE110KP112YQ114PE116DV118RR120SL122QE124AL126AL128RQ130DR132LL134RT136QR138LM140AI142DE144TL146A4459-5583phcR gene4,7404,7604,7804,8004,8204,8404,8604,8804,900
ttctcgcccacgaactgaacaccccgctggccgccatcgccaacttcgcgcgcggcatccgcatgcgcgtcgaccagaccgagccggccccggacacgctgcccggcatcggccgcgccgccgaccgcgtgcaggacaacgcgcgctactgcctgtcggtgctgtcgaccttcctaagagcgggtgcttgacttgtggggcgaccggcggtagcggttgaagcgcgcgccgtaggcgtacgcgcagctggtctggctcggccggggcctgtgcgacgggccgtagccggcgcggcggctggcgcacgtcctgttgcgcgcgatgacggacagccacgacagctggaaggaF148LA150HE152LN154TP156LA158AI160AN162FA164RG166IR168MR170VD172QT174EP176AP178DT180LP182GI184GR186AA188DR190VQ192DN194AR196YC198LS200VL202ST204FL2064459-5583phcR gene4,9204,9404,9604,9805,0005,0205,0405,060
gaattcggtgcggcacgcgcacggcaagtcggaagcggcgtcggcatcggcgcccagcagcgcagccggcctcatccgctcgctgctcgacacctatccgttcaccgcggtgcagcgcgattgggtgtcctaccggatcgagcgggacttcccgatcctgacgctgcccaactgccttaagccacgccgtgcgcgtgccgttcagccttcgccgcagccgtagccgcgggtcgtcgcgtcggccggagtaggcgagcgacgagctgtggataggcaagtggcgccacgtcgcgctaacccacaggatggcctagctcgccctgaagggctaggactgcgacgggttgacgNS208VR210HA212HG214KS216EA218AS220AS222AP224SS226AA228GL230IR232SL234LD236TY238PF240TA242VQ244RD246WV248SY250RI252ER254DF256PI258LT260LP262NC2644459-5583phcR gene5,0805,1005,1205,1405,1605,1805,2005,2205,240
gtgatgctggtgctgtcgtcgctgaccagcaacgcgctgcacgcgctgcggcataccgaggcgccacggctggagatcgtcgccgcgggcgcggcggcgctcaacggcacgcccggtgccggccatacgatccgcatcgaagacaacggccccggcatcgcgcccgacatcctggcactacgaccacgacagcagcgactggtcgttgcgcgacgtgcgcgacgccgtatggctccgcggtgccgacctctagcagcggcgcccgcgccgccgcgagttgccgtgcgggccacggccggtatgctaggcgtagcttctgttgccggggccgtagcgcgggctgtaggaccVM266LV268LS270SL272TS274NA276LH278AL280RH282TE284AP286RL288EI290VA292AG294AA296AL298NG300TP302GA304GH306TI308RI310ED312NG314PG316IA318PD320IL3224459-5583phcR gene5,2605,2805,3005,3205,3405,3605,3805,4005,420
cgcggctgaccatcgaccccgtgaccacgcgcgccgaagaaggcggcaatggcatgggcatgattttctgtacgcggatcatgcagtcgttcggaggcgggatcgccatcgattccgccccgagcgcaggcacgcgcgtgaccctgacgttcccatgaaccgagcgataccaggcgcgccgactggtagctggggcactggtgcgcgcggcttcttccgccgttaccgtacccgtactaaaagacatgcgcctagtacgtcagcaagcctccgccctagcggtagctaaggcggggctcgcgtccgtgcgcgcactgggactgcaagggtacttggctcgctatggtccgBsrDIAR324LT326ID328PV330TT332RA334EE336GG338NG340MG342MI344FC346TR348IM350QS352FG354GG356IA358ID360SA362PS364AG366TR368VT370LT372FP374*4459-5583phcR gene5,4405,4605,4805,5005,5205,5405,5605,5805,600
accacgccgtacctgatccgcgtttttgctaagaggagtgatggatgaccaaccccggggacaatgagcccaagatcctgtatgtggacgacgagacattggcgctgacctatttcggccgtgccatcggttcactggcaccggtcctcaccgccacctcggtcgaagaaggcaatggtgcggcatggactaggcgcaaaaacgattctcctcactacctactggttggggcccctgttactcgggttctaggacatacacctgctgctctgtaaccgcgactggataaagccggcacggtagccaagtgaccgtggccaggagtggcggtggagccagcttcttccgttAgeIBseRIMT2NP4GD6NE8PK10IL12YV14DD16ET18LA20LT22YF24GR26AI28GS30LA32PV34LT36AT38SV40EE42GK445645-6655phcQ - RSc27385,6205,6405,6605,6805,7005,7205,7405,760
gcgcatgctcgacgagcacgccgccacgctgggcgtgctggtgtcggaccagcgcatgcccggcgaactcggcaacgagctgctgcgctacgcgcgcgagcgctatccgcacatggtgcgtatcctgaccaccgcctactcggaaatcgaggacacggtggaggcggtcaaccagcgcgtacgagctgctcgtgcggcggtgcgacccgcacgaccacagcctggtcgcgtacgggccgcttgagccgttgctcgacgacgcgatgcgcgcgctcgcgataggcgtgtaccacgcataggactggtggcggatgagcctttagctcctgtgccacctccgccagttggtcDraIIIRM46LD48EH50AA52TL54GV56LV58SD60QR62MP64GE66LG68NE70LL72RY74AR76ER78YP80HM82VR84IL86TT88AY90SE92IE94DT96VE98AV100NQ1025645-6655phcQ - RSc27385,7805,8005,8205,8405,8605,8805,9005,9205,940
gggcagatccaccgctacatcaagaagccgtgggacatcaccgccctgcgggtcgaactcaagcaggcgctcgagttcgccgacctgcgccgcgagcgcgacgccctgctgcgcgagaagatgacggtgcggcagagccagacgctaacccatcgcatcggtgcgctgcagacgccccgtctaggtggcgatgtagttcttcggcaccctgtagtggcgggacgcccagcttgagttcgtccgcgagctcaagcggctggacgcggcgctcgcgctgcgggacgacgcgctcttctactgccacgccgtctcggtctgcgattgggtagcgtagccacgcgacgtctgcgTliIXhoIPspXIPaeR7IGQ104IH106RY108IK110KP112WD114IT116AL118RV120EL122KQ124AL126EF128AD130LR132RE134RD136AL138LR140EK142MT144VR146QS148QT150LT152HR154IG156AL158QT1605645-6655phcQ - RSc27385,9605,9806,0006,0206,0406,0606,0806,1006,120
tgtacctagcggtgtcggggctgtcgtcgttcagcgcagtcaacgactacctgcgggccgccgcggcggtcggcctgcacgcgctggaacagcccgactggacgctgaaggactacaccgaactggtcggcgaggaaagcctgcgtaccggcgagttcagcaacgccgtccggcaacatggatcgccacagccccgacagcagcaagtcgcgtcagttgctgatggacgcccggcggcgccgccagccggacgtgcgcgaccttgtcgggctgacctgcgacttcctgatgtggcttgaccagccgctcctttcggacgcatggccgctcaagtcgttgcggcaggccgtBfaILY162LA164VS166GL168SS170FS172AV174ND176YL178RA180AA182AV184GL186HA188LE190QP192DW194TL196KD198YT200EL202VG204EE206SL208RT210GE212FS214NA216VR218Q5645-6655phcQ - RSc27386,1406,1606,1806,2006,2206,2406,2606,2806,300
ggcgtaccaggacctgtcgacgcgcttcgacgccaaaccgcccgcgcaggcgctggaagtcctggcggaggtgctgcacggcaaggtctcgccggccgacgcaagcgcggcggcggtcgtggatctggcgacgctgacggaattcctggaggcatcgtcgacgcaccgcgtctcgccgcatggtcctggacagctgcgcgaagctgcggtttggcgggcgcgtccgcgaccttcaggaccgcctccacgacgtgccgttccagagcggccggctgcgttcgcgccgccgccagcacctagaccgctgcgactgccttaaggacctccgtagcagctgcgtggcgcagagcBsaXIEcoNISgrDIA220YQ222DL224ST226RF228DA230KP232PA234QA236LE238VL240AE242VL244HG246KV248SP250AD252AS254AA256AV258VD260LA262TL264TE266FL268EA270SS272TH274RV276S5645-6655phcQ - RSc27386,3206,3406,3606,3806,4006,4206,4406,460
acggcgcatgccgaatggctggccttcctgctctggctggacggcaagggctggtcggtcaccgccgagcgcgatggcgccaccgtcaagctgcgcctggtcgacaaccagtcccagctcacccccgcccagctcgccgcctggatggagcacttccaggagcagcagcaggccgtgccgcgtacggcttaccgaccggaaggacgagaccgacctgccgttcccgaccagccagtggcggctcgcgctaccgcggtggcagttcgacgcggaccagctgttggtcagggtcgagtgggggcgggtcgagcggcggacctacctcgtgaaggtcctcgtcgtcgtccggcBstEIIT278AH280AE282WL284AF286LL288WL290DG292KG294WS296VT298AE300RD302GA304TV306KL308RL310VD312NQ314SQ316LT318PA320QL322AA324WM326EH328FQ330EQ332QQ334A5645-6655phcQ - RSc27386,4806,5006,5206,5406,5606,5806,6006,6206,640
cctgaccggccaccccgcggcgccggcgctcaggatgcgtcggcgctgccgctgtcgggcgacggggcggactcgcccgccggacggtcgcgcataccgcccttgaccatctcgaaccggaacagccggcattcgatgttgccgttgtacagcggcgtgcgctgcgactccttgaggactggccggtggggcgccgcggccgcgagtcctacgcagccgcgacggcgacagcccgctgccccgcctgagcgggcggcctgccagcgcgtatggcgggaactggtagagcttggccttgtcggccgtaagctacaacggcaacatgtcgccgcacgcgacgctgaggaactBsmIMlyIMlyIPleIPleIA336*RSc27396,6606,6806,7006,7206,7406,7606,7806,8006,820
gccgcatccggcgcggcatcgacaggtcgccggtgaagacgaacgcctgccagccggcaaactcgcgcttgagcgtaccggcgaaagcctgcgcgaactcgttggccgcggcttcctcgcgcggatcgatgaccggcgcgggctccggctggcgcagctcgcgcgacggacggcgcggcgtaggccgcgccgtagctgtccagcggccacttctgcttgcggacggtcggccgtttgagcgcgaactcgcatggccgctttcggacgcgcttgagcaaccggcgccgaaggagcgcgcctagctactggccgcgcccgaggccgaccgcgtcgagcgcgctgcctgccgcRSc27396,8406,8606,8806,9006,9206,9406,9606,9807,000
cgggtcatcacgcccggcccccatcgcccggctggcccgctcgaagacggtgccgtcctgatccccggtctcgccttccggaccgcggcggcgcgagccgtgcgcaccgcgcaccacgatccgctcaccatacggcggattcatcagcagcacgcccggcgcctcgaacggcggcgcccagtagtgcgggccgggggtagcgggccgaccgggcgagcttctgccacggcaggactaggggccagagcggaaggcctggcgccgccgcgctcggcacgcgtggcgcgtggtgctaggcgagtggtatgccgcctaagtagtcgtcgtgcgggccgcggagcttgccgccgFspAIRSc27397,0207,0407,0607,0807,1007,1207,1407,160
ttgccgaagcgcgcatcgacctgcttgagcaccggctcgcccggcagccccgcgcggcgccagttggcggccgcataggccagcatgtcggtggagatgtcggcccccgcgatcagcggtgggcgatggcgcatggccgcgccggcggcatcggccgcggcttcgcgcaggcgctaacggcttcgcgcgtagctggacgaactcgtggccgagcgggccgtcggggcgcgccgcggtcaaccgccggcgtatccggtcgtacagccacctctacagccgggggcgctagtcgccacccgctaccgcgtaccggcgcggccgccgtagccggcgccgaagcgcgtccgcgaNotISfiIRSc27397,1807,2007,2207,2407,2607,2807,3007,3207,340
tgaagcgcttggcgtcgtggtcgcgcatccattcgcaagcgaaggcgcgctggccacccggcgcaacgcccagcgcgcgctgcgcggcttccaccaggaaggtgccgctgccgcacatcgggtcgtagaacggttggccctgggcacccgtccagccggtcaggcgcaggatgccacttcgcgaaccgcagcaccagcgcgtaggtaagcgttcgcttccgcgcgaccggtgggccgcgttgcgggtcgcgcgcgacgcgccgaaggtggtccttccacggcgacggcgtgtagcccagcatcttgccaaccgggacccgtgggcaggtcggccagtccgcgtcctacggPasIRSc27397,3607,3807,4007,4207,4407,4607,4807,5007,520
ggcagccaggttctccttgagcggcgcctcgcccttctcctggcgccagccgcgcttgaacagcggctcgccggtggtgtcgagatagagcgtgcagtcggtctcgctcaggtaggcgtagatgcgcacgtcgggcgagacggtgtccacgctgggacgcgcgccggcacgctggccgtcggtccaagaggaactcgccgcggagcgggaagaggaccgcggtcggcgcgaacttgtcgccgagcggccaccacagctctatctcgcacgtcagccagagcgagtccatccgcatctacgcgtgcagcccgctctgccacaggtgcgaccctgcgcgcggccgtgcgaccFspAIRSc27397,5407,5607,5807,6007,6207,6407,6607,6807,700
cggaagcggtcgcagatgccgtccttcacgcgcagcgcaacgaaattcaggctctgcagcggtgacttgtgcgccgtcacgtccacccggatggtctcgtcgggcgagaaatggtcttcccagcgctgcgcggcggtcaggctgtagatgtcgtcttccgagcggtagccgcgcggccttcgccagcgtctacggcaggaagtgcgcgtcgcgttgctttaagtccgagacgtcgccactgaacacgcggcagtgcaggtgggcctaccagagcagcccgctctttaccagaagggtcgcgacgcgccgccagtccgacatctacagcagaaggctcgccatcggcgcgcRSc27397,7207,7407,7607,7807,8007,8207,8407,860
cgccgaggcgcatcagcacccggctggcaatgcggctgtgcaggttgacggcgtaggccgcgccctgcgtgccgaagaagtgcacgccgcccggcacggtacggccgacctcgaacgcggccagctcgtccacctcggggcggatggcgatttcggccagctcatcggccagcgcgcggctccgcgtagtcgtgggccgaccgttacgccgacacgtccaactgccgcatccggcgcgggacgcacggcttcttcacgtgcggcgggccgtgccatgccggctggagcttgcgccggtcgagcaggtggagccccgcctaccgctaaagccggtcgagtagccggtcgcgBsrDIRSc27397,8807,9007,9207,9407,9607,9808,0008,0208,040
ctgttccagcccccgggggcacggtgcgaagtaagcgatcggcatcggacaaacagaaacagaagatcagaacggcttgaccacgaccaggatcgtgatcgccagcaacagcagcaccggcagttcgttgaaccagcggtagaacgtgtgcgagcgctgacaaggtcgggggcccccgtgccacgcttcattcgctagccgtagcctgtttgtctttgtcttctagtcttgccgaactggtgctggtcctagcactagcggtcgttgtcgtcgtggccgtcaagcaacttggtcgccatcttgcacacgctcgcgaPvuIRSc27398,0608,0808,1008,1208,1408,1608,1808,200
  • Linear Map
Editing disabled on read-only sequence.
tutorial: phcB - Rs GMI1000 (8208 bp)AgeIAgeIAloIAloIApaIAscIBaeIBclIBfaIBplIBplIBsmIBsmIBtsIBtsIFseIFseIMfeIMfeIMlyIMlyINcoINcoINotINruIPasIPasIPleIPleIPvuIPvuISacIScaIScaISfiISfiISrfITliITliIXhoIXhoIXmnIZraIZraIAatIIAatIIAsiSIBamHIBglIIBsaAIBsaAIBsaXIBsaXIBseRIBseRIBsiWIBspHIBsrDIBsrDIBtsαIBtsαIEcoNIEcoRVEcoRVFspAIFspAIPaqCIPflMIPflMIPspXIPspXIPvuIIPvuIISgrDIBstEIIBstEIIDraIIIDraIIIPaeR7IPaeR7IPspOMIEco53kI1,0002,0003,0004,0005,0006,0007,0008,0004459-55833122-44503122-44501705-31085645-6655RSc2739phcB genephcS genephcR geneRSc2734phcQ - RSc2738phcB UpstreamphcB Downstreamtutorial: phcB - Rs GMI1000 (8208 bp)

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8208