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pET-19b_MMLV-RT
  • Sequence Map
    Editing disabled on read-only sequence.
    ttcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgaaagaacttctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactZraIAatIIAmpR_promoter20406080100120140160
    taaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgaccSspIAmpicillinORF frame 2180200220240260280300320340
    atctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagttagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcacaactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcaScaITatIXmnIAmpicillinORF frame 2360380400420440460480500520
    cacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttPvuIAmpicillinORF frame 2540560580600620640660680700
    ccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacgtcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggAmpicillinORF frame 2720740760780800820840860
    ttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagAhdIBsaIAmpicillinORF frame 28809009209409609801,0001,0201,040
    actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttcAmpicillinORF frame 21,0601,0801,1001,1201,1401,1601,1801,2001,220
    atcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcg1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacgcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtg1,4201,4401,4601,4801,5001,5201,5401,560
    acagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttattgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaata1,5801,6001,6201,6401,6601,6801,7001,7201,740
    agtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataacctcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggDrdIPciIAflIII1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgcccataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatataccacgtgagagtcatgttagacgagactacggSapITatIBspQI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtccgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagAccIDrdIPfoIBsaAIPflFIBstZ17ITth111IpGEX_3_primerROP2,1202,1402,1602,1802,2002,2202,2402,260
    atcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcacttagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgaXmnIROP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcctacggaggcacattccccctaaagacaagtacccccattactatggctactttgctctctcctacgagtgctatgcccaatgactactacttgtacgggccaatgaccttgcaacactcccatttgttgaccgccatacctacgccgccctggtctctttttagtgagtcccag2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcattacggtcgcgaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgtaggacgctacgtctaggccttgtattaccacgtcccgcgactgaaggcgcaaaggtctgaaatgctttgtgcctttggcttctggtaagtacaacaacgagtccagcgtctgcaaaacgtBpu10I2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggccaggacccaacgctgcccgagatgcgccgcgtgcggctgctggagatggcggacgcgcgtcgtcagcgaagtgcaagcgagcgcatagccactaagtaagacgattggtcattccgttggggcggtcggatcggcccaggagttgctgtcctcgtgctagtacgcgtgggcaccggtcctgggttgcgacgggctctacgcggcgcacgccgacgacctctaccgcctgcgcMscIFspAI2,8202,8402,8602,8802,9002,9202,9402,960
    atggatatgttctgccaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagtacctatacaagacggttcccaaccaaacgcgtaagtgtcaagaggcgttcttaactaaccgaggttaagaacctcaccacttaggcaatcgctccacggcggccgaaggtaagtccagctccaccgggccgaggtacgtggcgctgcgttgcgcccctccgtctgttccatatctet (576 - 1190)ORF frame 32,9803,0003,0203,0403,0603,0803,1003,1203,140
    ggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccagtgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgccgccgcggatgttaggtacggttgggcaaggtacacgagcggctccgccgtatttagcggcactgctagtcgccaggtcactagcttcaatccgaccattctcggcgctcgctaggaacttcgacagggactaccagcagtagatggacggacctgtcgtaccggacgttgcgctet (576 - 1190)ORF frame 3ORF frame 13,1603,1803,2003,2203,2403,2603,2803,3003,320
    ggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggccgtagggctacggcggccttcgctcttcttagtattaccccttccggtaggtcggagcgcagcgcttgcggtcgttctgcatcgggtcgcgcagccggcggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccctggtcactgcttccgaactcgctcccEagINruItet (576 - 1190)ORF frame 3ORF frame 13,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgcgacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcgggtggccttcctPshAItet (576 - 1190)ORF frame 3ORF frame 13,5203,5403,5603,5803,6003,6203,6403,660
    gctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccORF frame 1ORF frame 2lacIORF frame 3tet (576 - 1190)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcggcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcBsaXIORF frame 2lacIORF frame 13,8603,8803,9003,9203,9403,9603,9804,0004,020
    tatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttatagggtgatggctctataggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaBssHIIORF frame 2lacI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaaApaIApoIPspOMIORF frame 2lacI4,2204,2404,2604,2804,3004,3204,3404,360
    gatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccctacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggBclIMluIAflIIIORF frame 2lacI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    atcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggclacIORF frame 2ORF frame 14,5604,5804,6004,6204,6404,6604,6804,7004,720
    cttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggtgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaORF frame 1lacI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcggcgttccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgPfoISphIEcoNIORF frame 14,9204,9404,9604,9805,0005,0205,0405,060
    gccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcactataggggcggctttgttcgcgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagctctagagctagggcgctttaattatgctgagtgatatccccBglIISgrAIpBRrevB...primerT7_promoterORF frame 15,0805,1005,1205,1405,1605,1805,2005,2205,240
    aattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatgggccatcatcatcatcatcatcatcatcatcacagcagcggccatatcgacgacgacgacaagcatATGGGCCAGCCGCTCCAAGTGCTCACCCTGAACATCGAAGttaacactcgcctattgttaaggggagatctttattaaaacaaattgaaattcttcctctatatggtacccggtagtagtagtagtagtagtagtagtagtgtcgtcgccggtatagctgctgctgctgttcgtaTACCCGGTCGGCGAGGTTCACGAGTGGGACTTGTAGCTTCBarINdeIXbaITL2NI4E5410-7461MG2HH4HH6HH8HH10HH12SS14GH16ID18DD20DK22HM24GQ26PL28QV30LT32LN34IE365317-7461Linker10x His TagMMLV-RT NdeI...R extensionlac operatorMMLV-RT...egmentT7_tr..._RBSMMLV ...tantEnt...se5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ACGAACATCGCTTGCACGAAACCAGCAAAGAACCGGATGTTTCACTGGGTTCCACTTGGCTGTCGGACTTTCCGCAAGCGTGGGCAGAAACCGGCGGTATGGGTCTGGCTGTGCGCCAAGCTCCGCTGATTATCCCACTGAAGGCAACTAGTACCCCGGTATCCATTAAACAGTATGCTTGTAGCGAACGTGCTTTGGTCGTTTCTTGGCCTACAAAGTGACCCAAGGTGAACCGACAGCCTGAAAGGCGTTCGCACCCGTCTTTGGCCGCCATACCCAGACCGACACGCGGTTCGAGGCGACTAATAGGGTGACTTCCGTTGATCATGGGGCCATAGGTAATTTGTCATBaeISpeID6EH8RL10HE12TS14KE16PD18VS20LG22ST24WL26SD28FP30QA32WA34ET36GG38MG40LA42VR44QA46PL48II50PL52KA54TS56TP58VS60IK62QY645410-7461DE38HR40LH42ET44SK46EP48DV50SL52GS54TW56LS58DF60PQ62AW64AE66TG68GM70GL72AV74RQ76AP78LI80IP82LK84AT86ST88PV90SI92KQ94Y5317-7461MMLV-RT NdeI front.FOR extensionMMLV RT mutant5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CCCGATGAGCCAGGAGGCCCGTCTGGGCATTAAACCGCATATCCAGCGTCTGCTGGACCAGGGTATCTTAGTCCCGTGCCAGTCCCCGTGGAACACCCCGCTGCTGCCGGTTAAAAAACCGGGCACCAACGACTATCGCCCGGTACAAGATTTACGTGAAGTTAACAAGCGCGTAGGGCTACTCGGTCCTCCGGGCAGACCCGTAATTTGGCGTATAGGTCGCAGACGACCTGGTCCCATAGAATCAGGGCACGGTCAGGGGCACCTTGTGGGGCGACGACGGCCAATTTTTTGGCCCGTGGTTGCTGATAGCGGGCCATGTTCTAAATGCACTTCAATTGTTCGCGCATBaeIBsaAIPM66SQ68EA70RL72GI74KP76HI78QR80LL82DQ84GI86LV88PC90QS92PW94NT96PL98LP100VK102KP104GT106ND108YR110PV112QD114LR116EV118NK120RV1225410-7461P96MS98QE100AR102LG104IK106PH108IQ110RL112LD114QG116IL118VP120CQ122SP124WN126TP128LL130PV132KK134PG136TN138DY140RP142VQ144DL146RE148VN150KR152V5317-7461MMLV-RT NdeI front.FOR extensionMMLV RT mutant5,6205,6405,6605,6805,7005,7205,7405,760
    GAAGATATCCATCCGACCGTTCCCAACCCGTACAATCTGCTCTCAGGTCCGCCGCCTTCCCACCAATGGTACACCGTTCTGGACCTGAAAGATGCCTTCTTTTGCTTGCGTCTGCATCCGACTAGCCAGCCCCTGTTTGCATTCGAGTGGCGTGACCCGGAAATGGGGATCAGCGCTTCTATAGGTAGGCTGGCAAGGGTTGGGCATGTTAGACGAGAGTCCAGGCGGCGGAAGGGTGGTTACCATGTGGCAAGACCTGGACTTTCTACGGAAGAAAACGAACGCAGACGTAGGCTGATCGGTCGGGGACAAACGTAAGCTCACCGCACTGGGCCTTTACCCCTAGTCGCED124IH126PT128VP130NP132YN134LL136SG138PP140PS142HQ144WY146TV148LD150LK152DA154FF156CL158RL160HP162TS164QP166LF168AF170EW172RD174PE176MG178IS1805410-7461E154DI156HP158TV160PN162PY164NL166LS168GP170PP172SH174QW176YT178VL180DL182KD184AF186FC188LR190LH192PT194SQ196PL198FA200FE202WR204DP206EM208GI210S5317-7461MMLV-RT NdeI front.FOR extensionMMLV RT mutantMMLV-RT 1:2.FOR extension5,7805,8005,8205,8405,8605,8805,9005,9205,940
    GCCAGCTCACCTGGACCCGTCTGCCGCAAGGTTTCAAAAACTCACCTACCCTGTTTAATGAAGCTCTGCACCGCGATCTGGCGGATTTCCGCATTCAACACCCGGATCTGATTCTGCTGCAGTATGTGGACGACCTGCTGCTGGCGGCTACCTCCGAACTGGATTGCCAGCAGGGCGGTCGAGTGGACCTGGGCAGACGGCGTTCCAAAGTTTTTGAGTGGATGGGACAAATTACTTCGAGACGTGGCGCTAGACCGCCTAAAGGCGTAAGTTGTGGGCCTAGACTAAGACGACGTCATACACCTGCTGGACGACGACCGCCGATGGAGGCTTGACCTAACGGTCGTCCCGQ182LT184WT186RL188PQ190GF192KN194SP196TL198FN200EA202LH204RD206LA208DF210RI212QH214PD216LI218LL220QY222VD224DL226LL228AA230TS232EL234DC236QQ238G5410-7461G212QL214TW216TR218LP220QG222FK224NS226PT228LF230NE232AL234HR236DL238AD240FR242IQ244HP246DL248IL250LQ252YV254DD256LL258LA260AT262SE264LD266CQ268QG2705317-7461MMLV RT mutantMMLV-RT 1:2.FOR extensionMMLV-RT NdeI front.FOR extension5,9605,9806,0006,0206,0406,0606,0806,1006,120
    CACCCGTGCCTTACTGCAAACCCTGGGTAACTTAGGTTATCGTGCTAGCGCGAAAAAAGCCCAGATCTGTCAGAAGCAGGTTAAATACCTGGGTTACCTCCTGAAAGAAGGCCAGCGCTGGTTAACCGAAGCTCGCAAGGAGACGGTCATGGGTCAGCCAACCCCGAAAACCCCGGTGGGCACGGAATGACGTTTGGGACCCATTGAATCCAATAGCACGATCGCGCTTTTTTCGGGTCTAGACAGTCTTCGTCCAATTTATGGACCCAATGGAGGACTTTCTTCCGGTCGCGACCAATTGGCTTCGAGCGTTCCTCTGCCAGTACCCAGTCGGTTGGGGCTTTTGGGGCBmtINheIPasIBglIIT240RA242LL244QT246LG248NL250GY252RA254SA256KK258AQ260IC262QK264QV266KY268LG270YL272LK274EG276QR278WL280TE282AR284KE286TV288MG290QP292TP294KT296P5410-7461TR272AL274LQ276TL278GN280LG282YR284AS286AK288KA290QI292CQ294KQ296VK298YL300GY302LL304KE306GQ308RW310LT312EA314RK316ET318VM320GQ322PT324PK326TP3285317-7461MMLV RT mutantMMLV-RT 1:2.FOR extension6,1406,1606,1806,2006,2206,2406,2606,2806,300
    CGCCAGCTGCGTGAGTTCCTGGGCACCGCGGGCTTTTGTCGCCTGTGGATCCCGGGCTTCGCGGAAATGGCTGCGCCGCTGTACCCGCTCACCAAACCGGGGACCCTGTTCAACTGGGGTCCGGATCAGCAAAAGGCTTACCAGGAGATCAAACAGGCGTTGCTGACCGCACCAGGCGGTCGACGCACTCAAGGACCCGTGGCGCCCGAAAACAGCGGACACCTAGGGCCCGAAGCGCCTTTACCGACGCGGCGACATGGGCGAGTGGTTTGGCCCCTGGGACAAGTTGACCCCAGGCCTAGTCGTTTTCCGAATGGTCCTCTAGTTTGTCCGCAACGACTGGCGTGGTCKflISmaIXmaIBamHISacIITspMIR298QL300RE302FL304GT306AG308FC310RL312WI314PG316FA318EM320AA322PL324YP326LT328KP330GT332LF334NW336GP338DQ340QK342AY344QE346IK348QA350LL352TA354P5410-7461RQ330LR332EF334LG336TA338GF340CR342LW344IP346GF348AE350MA352AP354LY356PL358TK360PG362TL364FN366WG368PD370QQ372KA374YQ376EI378KQ380AL382LT384AP3865317-7461MMLV RT mutantMMLV-RT 1:2.FOR extensionMMLV-RT 2:3.FOR extension6,3206,3406,3606,3806,4006,4206,4406,460
    CTCTCGGTCTGCCGGACCTGACGAAACCGTTTGAACTGTTTGTGGACGAAAAACAGGGTTACGCAAAAGGTGTTCTGACCCAGAAACTGGGTCCATGGCGTCGCCCGGTCGCCTACCTGAGCAAGAAACTGGACCCGGTTGCAGCTGGCTGGCCGCCGTGTCTCCGTATGGTAGCGAGAGCCAGACGGCCTGGACTGCTTTGGCAAACTTGACAAACACCTGCTTTTTGTCCCAATGCGTTTTCCACAAGACTGGGTCTTTGACCCAGGTACCGCAGCGGGCCAGCGGATGGACTCGTTCTTTGACCTGGGCCAACGTCGACCGACCGGCGGCACAGAGGCATACCATCGBpu10IA356LG358LP360DL362TK364PF366EL368FV370DE372KQ374GY376AK378GV380LT382QK384LG386PW388RR390PV392AY394LS396KK398LD400PV402AA404GW406PP408CL410RM412VA4145410-7461AL388GL390PD392LT394KP396FE398LF400VD402EK404QG406YA408KG410VL412TQ414KL416GP418WR420RP422VA424YL426SK428KL430DP432VA434AG436WP438PC440LR442MV444A5317-7461MMLV RT mutantMMLV-RT 2:3.FOR extensionMMLV-RT 1:2.FOR extension6,4806,5006,5206,5406,5606,5806,6006,6206,640
    TGCAATCGCTGTGCTGACCAAAGATGCGGGAAAACTGACCATGGGTCAACCGTTGGTGATCCTGGCACCGCATGCGGTAGAAGCCCTGGTGAAACAGCCGCCGGACCGTTGGCTGTCCAACGCCCGCATGACGCACTACCAGGCGCTGCTGCTGGATACCGATCGTGTTCAGTTCACGTTAGCGACACGACTGGTTTCTACGCCCTTTTGACTGGTACCCAGTTGGCAACCACTAGGACCGTGGCGTACGCCATCTTCGGGACCACTTTGTCGGCGGCCTGGCAACCGACAGGTTGCGGGCGTACTGCGTGATGGTCCGCGACGACGACCTATGGCTAGCACAAGTCAAGAhdIPvuISphIRsrIIAI416AV418LT420KD422AG424KL426TM428GQ430PL432VI434LA436PH438AV440EA442LV444KQ446PP448DR450WL452SN454AR456MT458HY460QA462LL464LD466TD468RV470QF4725410-7461A446IA448VL450TK452DA454GK456LT458MG460QP462LV464IL466AP468HA470VE472AL474VK476QP478PD480RW482LS484NA486RM488TH490YQ492AL494LL496DT498DR500VQ502F5317-7461MMLV RT mutantMMLV-RT 2:3.FOR extension6,6606,6806,7006,7206,7406,7606,7806,8006,820
    GGCCCGGTTGTTGCGCTGAACCCGGCAACTCTGCTTCCGTTACCGGAAGAGGGTCTGCAGCACAACTGCCTTGATATCCTCGCAGAAGCGCACGGCACCCGTCCTGACCTGACGGACCAGCCACTGCCGGACGCAGATCATACCTGGTACACCGACGGCAGCAGCCTGCTGCAAGCCGGGCCAACAACGCGACTTGGGCCGTTGAGACGAAGGCAATGGCCTTCTCCCAGACGTCGTGTTGACGGAACTATAGGAGCGTCTTCGCGTGCCGTGGGCAGGACTGGACTGCCTGGTCGGTGACGGCCTGCGTCTAGTATGGACCATGTGGCTGCCGTCGTCGGACGACGTTCSexAIGP474VV476AL478NP480AT482LL484PL486PE488EG490LQ492HN494CL496DI498LA500EA502HG504TR506PD508LT510DQ512PL514PD516AD518HT520WY522TD524GS526SL528LQ5305410-7461G504PV506VA508LN510PA512TL514LP516LP518EE520GL522QH524NC526LD528IL530AE532AH534GT536RP538DL540TD542QP544LP546DA548DH550TW552YT554DG556SS558LL560Q5317-7461MMLV RT mutantMMLV-RT 2:3.FOR extensionMMLV-RT 3:XholI.FOR extension6,8406,8606,8806,9006,9206,9406,9606,9807,000
    AGGGCCAACGCAAAGCGGGGGCAGCCGTAACCACTGAGACCGAGGTTATTTGGGCTAAAGCTCTGCCAGCCGGCACTTCTGCACAGCGTGCGGAACTGATCGCTCTGACCCAAGCCCTGAAAATGGCTGAAGGCAAGAAATTGAACGTTTACACCGACAGCCGCTATGCGTTCGCTCCCGGTTGCGTTTCGCCCCCGTCGGCATTGGTGACTCTGGCTCCAATAAACCCGATTTCGAGACGGTCGGCCGTGAAGACGTGTCGCACGCCTTGACTAGCGAGACTGGGTTCGGGACTTTTACCGACTTCCGTTCTTTAACTTGCAAATGTGGCTGTCGGCGATACGCAAGCGBsaINruIDraIIIEG532QR534KA536GA538AV540TT542ET544EV546IW548AK550AL552PA554GT556SA558QR560AE562LI564AL566TQ568AL570KM572AE574GK576KL578NV580YT582DS584RY586AF588A5410-7461E562GQ564RK566AG568AA570VT572TE574TE576VI578WA580KA582LP584AG586TS588AQ590RA592EL594IA596LT598QA600LK602MA604EG606KK608LN610VY612TD614SR616YA618FA6205317-7461MMLV RT mutantMMLV-RT 3:XholI.FOR extensionMMLV-RT 2:3.FOR extension7,0207,0407,0607,0807,1007,1207,1407,160
    GACTGCCCACATCCACGGTGAAATCTACCGCCGCCGCGGTTGGCTGACGTCGAAGGGCAAAGAAATCAAAAACAAAGATGAAATCTTAGCTCTGCTGAAAGCTCTGTTTCTGCCGAAACGTCTGTCCATTATTCACTGCCCGGGTCACCAGAAAGGGCACTCTGCAGAAGCTCGTCTGACGGGTGTAGGTGCCACTTTAGATGGCGGCGGCGCCAACCGACTGCAGCTTCCCGTTTCTTTAGTTTTTGTTTCTACTTTAGAATCGAGACGACTTTCGAGACAAAGACGGCTTTGCAGACAGGTAATAAGTGACGGGCCCAGTGGTCTTTCCCGTGAGACGTCTTCGAGCASmaIXmaIZraIAatIISacIITspMIT590AH592IH594GE596IY598RR600RG602WL604TS606KG608KE610IK612NK614DE616IL618AL620LK622AL624FL626PK628RL630SI632IH634CP636GH638QK640GH642SA644EA646R5410-7461TA622HI624HG626EI628YR630RR632GW634LT636SK638GK640EI642KN644KD646EI648LA650LL652KA654LF656LP658KR660LS662II664HC666PG668HQ670KG672HS674AE676AR6785317-7461MMLV RT mutantMMLV-RT 3:XholI.FOR extension7,1807,2007,2207,2407,2607,2807,3007,3207,340
    GGAAACCGTATGGCAGACCAGGCTGCGCGTAAAGCAGCCATCACTGAAACCCCGGATACGTCAACTCTGCTGATTGAAAACAGCAGCCCGAACAGCCGCTTGATTAACTGACtcgaTgatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcCCTTTGGCATACCGTCTGGTCCGACGCGCATTTCGTCGGTAGTGACTTTGGGGCCTATGCAGTTGAGACGACTAACTTTTGTCGTCGGGCTTGTCGGCGAACTAATTGACTGagctActaggccgacgattgtttcgggctttccttcgactcaaccgacgacggtggcgactcgBlpIG648NR650MA652DQ654AA656RK658AA660IT662ET664PD666TS668TL670LI672EN674SS676PN678SR680LI682N*6845410-7461GN680RM682AD684QA686AR688KA690AI692TE694TP696DT698ST700LL702IE704NS706SP708NS710RL712IN714*5317-7461MMLV-RT 3:XholI.FOR extensionMMLV RT mutantT7_terminatorMCS7,3607,3807,4007,4207,4407,4607,4807,5007,520
    aataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttcctccttgatataggcctatagggcgttctccgggccgtcatggccgtattggttcggatacggatgtcgtaggtcccactgccacggctcctactgctactcgcgtaacaatctaT7_terminatorT7...r7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ttcatacacggtgcctgactgcgttagcaatttaactgtgataaactaccgcattaaagcttatcgatgataagctgtcaaacatgagaaaagtatgtgccacggactgacgcaatcgttaaattgacactatttgatggcgtaatttcgaatagctactattcgacagtttgtactcttApoIClaIBspDIEcoRIHindIII7,7207,7407,7607,780
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7790 bp5500600065007000750050010001500AmpicillinORF frame 2CCTGG5410-74615317-7461AmpR_promoter,ZraI,AatII,SspIXmnITatI,ScaI,PvuIBsaIAhdIDrdIAflIIIPciIGATC,CCTGG,D200N,GATCGATCCCTGG,GATC,CCTGG,PasI,NheI,BmtI,BglIIMMLV-RT 1:2.FOR extension,CCTGG,SacII,BamHIGATCMMLV RT mutant,T330P,GATC,CCTGG,GATC,TspMI,XmaI,SmaI,KflIBpu10I,AhdIMMLV-RT 2:3.FOR extension,GATC,CCTGG,CCTGG,CCTGG,GATC,SphI,RsrII,+1GATC,SexAIGATC,BsaI,DraIIIMMLV-RT 3:XholI.FOR extension,L603W,E607K,CCTGG,NruI,SacII,ZraI,AatII,+3MCS,MMLV-RT 3:XholI.FOR extension,MCS,T7_terminator,T7_Terminal_primer,BlpI,+5

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    7790