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XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttaSpeICMVCMV promoter (with TetOs)20406080100120140160
    agggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccatatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaCspCICMVCMV promoter (with TetOs)360380400420440460480500520
    tgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctBbsISacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    ccgatccagcctccggactctagcgtttaaacttaagctcgctagagatccgcggccgctaatacgactcactatagggagaaaaataacaaatctcaacacaacatatacaaaacaaacgaatctcaagcaatcaagcattctacttctattgcagcaatttaaatcatttcttttaggctaggtcggaggcctgagatcgcaaatttgaattcgagcgatctctaggcgccggcgattatgctgagtgatatccctctttttattgtttagagttgtgttgtatatgttttgtttgcttagagttcgttagttcgtaagatgaagataacgtcgttaaatttagtaaagaaaatBsmINotIPmeISwaIAflIISacIITEV leader UTR5T7 inserthomologCMV ...Os)720740760780800820840860880
    aagcaaaagcaattttctgaaaattttcaccatttacgaacgataAAGCTTcgccaccATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACttcgttttcgttaaaagacttttaaaagtggtaaatgcttgctatTTCGAAgcggtggTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGBseRIHindIIIM1VS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VS30GE32GE34GD36AT38Y952-1665EGFP (encoding from LEAPER)TEV leader UTR5homolog9009209409609801,0001,0201,0401,060
    GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98952-1665EGFP (encoding from LEAPER)1,0801,1001,1201,1401,1601,1801,2001,2201,240
    TCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTFF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144YN146SH148NV150YI152MA154DK156QK158952-1665EGFP (encoding from LEAPER)1,2601,2801,3001,3201,3401,3601,3801,4001,420
    GAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGNG160IK162VN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200LS202TQ204SA206LS208KD210PN212EK214RD216H952-1665EGFP (encoding from LEAPER)1,4401,4601,4801,5001,5201,5401,5601,5801,600
    ATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTATAAGgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccgTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATATTCctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcKpnIMluIAcc65IE1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331675-1773D1A2L3M218VL220LE222FV224TA226AG228IT230LG232MD234EL236YK238952-1665G1T25' StopGo (2A; Loughran 2017)EGFP (encoding from LEAPER)homolog1,6201,6401,6601,6801,7001,7201,7401,7601,780
    gatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAActaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTAgeIBaeISpeIBamHIT1S2G1S2T1G2E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N171909-2007S1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T371786-1896ADAR substrate3xMS2 SGR3' StopGo (2A; Loughran 2017)5' stemloop13' stemloop2loop31,8001,8201,8401,8601,8801,9001,9201,940
    CTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTatggccaatttactgaccgtacaccaaaatttgcctgcattGccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcataccGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCAtaccggttaaatgactggcatgtggttttaaacggacgtaaCggccagctacgttgctcactactccaagcgttcttggactacctgtacaagtccctagcggtccgcaaaagactcgtatggMscIPciITliIXhoIPspXIPaeR7IAN2LL4TV6HQ8NL10PA12LP14VD16AT18SD20EV22RK24NL26MD28MF30RD32RQ34AF36SE38HT402017-3042M1F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331909-2007S1S2Cre3' StopGo (2A; Loughran 2017)homolog1,9601,9802,0002,0202,0402,0602,0802,1002,120
    tggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcaccttttacgaagacaggcaaacggccagcacccgccgtaccacgttcaacttattggcctttaccaaagggcgtcttggacttctacaagcgctaatagaagatatagaagtccgcgcgccagaccgtcatttttgataggtcgttgtaaacccggtcgatttgtacgaagtagcagNruIBssHIIWK42ML44LS46VC48RS50WA52AW54CK56LN58NR60KW62FP64AE66PE68DV70RD72YL74LY76LQ78AR80GL82AV84KT86IQ88QH90LG92QL94NM96LH98R2017-3042Cre2,1402,1602,1802,2002,2202,2402,2602,2802,300
    ggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatcAgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaaccaggcccgacggtgctggttcactgtcgttacgacaaagtgaccaatacgccgcctagTcttttcttttgcaactacggccacttgcacgttttgtccgagatcgcaagcttgcgtgactaaagctggtccaagcaagtgagtaccttttatcgctagcgacggtcctatatgcattAsiSIBstBIRsrIISexAIR100SG102LP104RP106SD108SN110AV112SL114VM116RR118IR120KE122NV124DA126GE128RA130KQ132AL134AF136ER138TD140FD142QV144RS146LM148EN150SD152RC154QD156IR158N2017-3042Cre2,3202,3402,3602,3802,4002,4202,4402,4602,480
    tctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaaagaccgtaaagacccctaacgaatattgtgggacaatgcatatcggctttaacggtcctagtcccaatttctatagagtgcatgactgccaccctcttacaattaggtataaccgtcttgcttttgcgaccaatcgtggcgtccacatctcttccgtgaatcggacccccattgatttPsiIEcoRVL160AF162LG164IA166YN168TL170LR172IA174EI176AR178IR180VK182DI184SR186TD188GG190RM192LI194HI196GR198TK200TL202VS204TA206GV208EK210AL212SL214GV216TK2182017-3042Cre2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ctggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactgaccagctcgctacctaaaggcagagaccacatcgactactaggcttattgatggacaaaacggcccagtcttttttaccacaacggcgcggtagacggtggtcggtcgatagttgagcgcgggaccttccctaaaaacttcgttgagtagctaactaaatgccgcgattcctactgaClaIBspDILV220ER222WI224SV226SG228VA230DD232PN234NY236LF238CR240VR242KN244GV246AA248PS250AT252SQ254LS256TR258AL260EG262IF264EA266TH268RL270IY272GA274KD276D2017-3042Cre2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgaccagtctctatggaccggaccagacctgtgtcacgggcacagcctcggcgcgctctataccgggcgcgacctcaaagttatggcctctagtacgttcgaccaccgacctggttacatttataacagtacttgatataggcattggacctatcactttgtccccgttaccacgcggaSspIPshAIS278GQ280RY282LA284WS286GH288SA290RV292GA294AR296DM298AR300AG302VS304IP306EI308MQ310AG312GW314TN316VN318IV320MN322YI324RN326LD328SE330TG332AM334VR336L2017-3042Cre2,8602,8802,9002,9202,9402,9602,9803,0003,020
    gctggaagatggcgattgaCTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCcgaccttctaccgctaactGAGCTCGACCATGACGTACGTGCGTTACGATCGACGGGGAAAGGGCAGGACCCATGGGGCTCAGAGGGGGCTGGAGCCCAGGGTCCATACGAGGGTGGAGGTGGACGGGGTGAGTGGTGGAGACGATCAAGGTCTGTGGAGGGTTCGTGCGTCGTTACGBmtIKflIKpnINheISphITliIXhoIPspXIAcc65IPaeR7IL338ED340GD3422017-3042*1hybrid UTR3Crehomolog3,0403,0603,0803,1003,1203,1403,1603,1803,200
    AGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCgggcccgaacaaaaactcatctcagaagaggatctgaatagcTCGAGTTTTGCGAATCGGATCGGTGTGGGGGTGCCCTTTGTCGTCACTAATTGGAAATCGTTATTTGCTTTCAAATTGATTCGATATGATTGGGGTCCCAACCAGTTAAAGCACGGTCGGTGTGGGACCTCGATCGcccgggcttgtttttgagtagagtcttctcctagacttatcgApaIBlpIBmtINheIPasIXcmIPflMIPspOMIhybrid UTR3c-myc epitopehomolog3,2203,2403,2603,2803,3003,3203,3403,3603,380
    gccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgacggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactBclIPmeIBGH polyadenylation sequencePolyhi...e tag3,4003,4203,4403,4603,4803,5003,5203,5403,560
    gtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctaggggcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatccccBbsIClaISphIBspDIPvuIIBGH polyadenylation sequenceBGH pA3,5803,6003,6203,6403,6603,6803,7003,720
    gtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggtcataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaKflINaeINgoMIV3,7403,7603,7803,8003,8203,8403,8603,8803,900
    tccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctBarIMscIXbaIBsaXIPshAIHygromycinFRT3,9203,9403,9603,9804,0004,0204,0404,0604,080
    cagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgPflFITth111IHygromycin4,1004,1204,1404,1604,1804,2004,2204,2404,260
    attggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgctaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgPstIAsiSIEcoRIRsrIIDraIIIHygromycin4,2804,3004,3204,3404,3604,3804,4004,4204,440
    aaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggattccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctNdeIPflFIDraIIITth111IHygromycin4,4604,4804,5004,5204,5404,5604,5804,6004,620
    tttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtAjuIHygromycin4,6404,6604,6804,7004,7204,7404,7604,7804,800
    ggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagaSacIIHygromycin4,8204,8404,8604,8804,9004,9204,9404,9604,980
    ggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctNaeIScaINgoMIVHygromycin5,0005,0205,0405,0605,0805,1005,1205,1405,160
    tctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagcagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgBsmIPsiIBstZ17ISV40 pA5,1805,2005,2205,2405,2605,2805,3005,3205,340
    tagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggaca5,3605,3805,4005,4205,4405,4605,4805,500
    cgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcagggcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtccSapIBsaXIBspQIPvuII5,5205,5405,5605,5805,6005,6205,6405,6605,680
    aaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgaggPciIpUC ori5,7005,7205,7405,7605,7805,8005,8205,8405,860
    ctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctgagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggapUC ori5,8805,9005,9205,9405,9605,9806,0006,0206,040
    tatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgapUC ori6,0606,0806,1006,1206,1406,1606,1806,2006,220
    gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtcttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcapUC ori6,2406,2606,2806,3006,3206,3406,3606,3806,400
    taagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacAmp (bla)6,4206,4406,4606,4806,5006,5206,5406,5606,580
    cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagBsaIAmp (bla)6,6006,6206,6406,6606,6806,7006,7206,7406,760
    tattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcgataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgcFspIAmp (bla)6,7806,8006,8206,8406,8606,8806,9006,9206,940
    gttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttcaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaaScaIAmp (bla)6,9606,9807,0007,0207,0407,0607,0807,1007,120
    gctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtgAmp (bla)7,1407,1607,1807,2007,2207,2407,2607,280
    cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatagtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatSspIbla promoterAmp (bla)7,3007,3207,3407,3607,3807,4007,4207,4407,460
    ggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagcccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgSgrDI7,4807,5007,5207,5407,5607,5807,6007,6207,640
    tacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWI7,6607,6807,7007,7207,740
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7744 bp5500600065007000500100015003' stemmutation siteopposite edited A1909-2007EKo437.2 (DNA)EKo438.4 (DNA)pNK018 (DNA)M13-48REV Genewiz (DNA)CMV,SpeI,NdeICMV promoter (with TetOs),A,CMV-Forward Genewiz (DNA),CspCITATA box,TetO,TetR binding sites,TetO,CMVF1 (DNA),ToFlpF (DNA),+8homolog,HindIII residue,T7 insert,XZp072.1 (DNA),PmeI,AflII,NotI,+2TEV leader UTR5,homolog,kozak,949-951,XZp388.1 (DNA),SwaI,+1EGFP-N Genewiz (DNA),M-SPOTIT-seq-F2 (DNA),Cit2 (DNA),BseRICit5 (DNA)mTur_2 (don't have?) (DNA),EKo433.1 (DNA)EGFP (encoding from LEAPER),952-1665f-egfp-2-qPCR (DNA)r-egfp-2-qPCR (DNA)f-egfp-1-qPCR (DNA),Cit7 (DNA),r-egfp-1-qPCR (DNA),+1homolog,+75' StopGo (2A; Loughran 2017),1675-1773,1774-1779,+35' stem,ADAR substrate,edited,bulged A -- edited?,+12loop3,loop MS2,bulged,bulge opposite the other A,+93' StopGo (2A; Loughran 2017),XZp388.4 (DNA)pUCoriR (DNA)pUC oriEKo152.2 (DNA)BsaIFspIAmp (bla)ScaISspIbla promoter,FlpInF1 (DNA),FlpInF (DNA),EKo152.4 (DNA),SgrDI,MfeI,BsiWI,MluI

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    7744