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XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagctcgctagagatccgcggccgctaatacgactcactatagggagaaaaataacaaatctcaacacaacatatacaaaacaaacgaatctcaagcaatcaagcattctacttctattgcagcaatttttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgagcgatctctaggcgccggcgattatgctgagtgatatccctctttttattgtttagagttgtgttgtatatgttttgtttgcttagagttcgttagttcgtaagatgaagataacgtcgttaaaBbsIBsmINotIPmeIAflIISacIITEV leader UTR5T7 insertCMV promot...th TetOs)homolog720740760780800820840860
    aaatcatttcttttaaagcaaaagcaattttctgaaaattttcaccatttacgaacgataAAGCTTcgccaccATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCtttagtaaagaaaatttcgttttcgttaaaagacttttaaaagtggtaaatgcttgctatTTCGAAgcggtggTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGSwaIBseRIHindIIIVS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VS30GE32G952-1665M1EGFP (encoding from LEAPER)TEV leader UTR5homolog8809009209409609801,0001,0201,040
    GAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGAE34GD36AT38YG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84KS86AM88PE90G952-1665EGFP (encoding from LEAPER)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTTGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAY92VQ94ER96TI98FF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144YN146SH148NV150952-1665EGFP (encoding from LEAPER)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGYI152MA154DK156QK158NG160IK162VN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200LS202TQ204SA206LS208952-1665EGFP (encoding from LEAPER)1,4201,4401,4601,4801,5001,5201,5401,560
    AAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTATAAGgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATATTCctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCMluID1A2L3E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G251675-1773KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228IT230LG232MD234EL236YK238952-1665EGFP (encoding from LEAPER)5' StopGo (2A; Loughran 2017)homolog1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCATGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTAgeIBaeIKpnISpeIBamHIAcc65IG1T2S1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T371786-1896E1A2R3H4K5Q61909-2007T1G2G1S2T1S2D26V27E28S29N30P31G32P331675-1773ADAR substrate3xMS2 SGR5' stem5' StopG...n 2017)loop13' stemloop2loop33' St...017)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    AAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTatggccaatttactgaccgtacaccaaaatttgcctgcattGccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCAtaccggttaaatgactggcatgtggttttaaacggacgtaaCggccagctacgttgctcactactccaagcgttcttggactacctgMscIPciITliIXhoIPspXIPaeR7IK7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331909-2007S1S2AN2LL4TV6HQ8NL10PA12LP14VD16AT18SD20EV22RK24NL26MD282017-3042M1Cre3' StopGo (2A; Loughran 2017)homolog1,9401,9601,9802,0002,0202,0402,0602,0802,100
    atgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatacaagtccctagcggtccgcaaaagactcgtatggaccttttacgaagacaggcaaacggccagcacccgccgtaccacgttcaacttattggcctttaccaaagggcgtcttggacttctacaagcgctaatagaagatatagaagtccgcgcgccagaccgtcatttttgatNruIBssHIIMF30RD32RQ34AF36SE38HT40WK42ML44LS46VC48RS50WA52AW54CK56LN58NR60KW62FP64AE66PE68DV70RD72YL74LY76LQ78AR80GL82AV84KT862017-3042Cre2,1202,1402,1602,1802,2002,2202,2402,260
    tccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatcAgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgaggtcgttgtaaacccggtcgatttgtacgaagtagcagccaggcccgacggtgctggttcactgtcgttacgacaaagtgaccaatacgccgcctagTcttttcttttgcaactacggccacttgcacgttttgtccgagatcgcaagcttgcgtgactaaagctggtccaagcBstBIRsrIISexAIIQ88QH90LG92QL94NM96LH98RR100SG102LP104RP106SD108SN110AV112SL114VM116RR118IR120KE122NV124DA126GE128RA130KQ132AL134AF136ER138TD140FD142QV144R2017-3042Cre2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttaagtgagtaccttttatcgctagcgacggtcctatatgcattagaccgtaaagacccctaacgaatattgtgggacaatgcatatcggctttaacggtcctagtcccaatttctatagagtgcatgactgccaccctcttacaattaggtataaccgtcttgcttttgcgaccaaPsiIAsiSIEcoRVS146LM148EN150SD152RC154QD156IR158NL160AF162LG164IA166YN168TL170LR172IA174EI176AR178IR180VK182DI184SR186TD188GG190RM192LI194HI196GR198TK200TL202V2017-3042Cre2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagtcgtggcgtccacatctcttccgtgaatcggacccccattgatttgaccagctcgctacctaaaggcagagaccacatcgactactaggcttattgatggacaaaacggcccagtcttttttaccacaacggcgcggtagacggtggtcggtcgatagttgagcgcgggaccttcS204TA206GV208EK210AL212SL214GV216TK218LV220ER222WI224SV226SG228VA230DD232PN234NY236LF238CR240VR242KN244GV246AA248PS250AT252SQ254LS256TR258AL260E2017-3042Cre2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcctaaaaacttcgttgagtagctaactaaatgccgcgattcctactgagaccagtctctatggaccggaccagacctgtgtcacgggcacagcctcggcgcgctctataccgggcgcgacctcaaagttatggcctctagtacgttcgaccaccgacctggttacatttataacaClaISspIBspDIPshAIG262IF264EA266TH268RL270IY272GA274KD276DS278GQ280RY282LA284WS286GH288SA290RV292GA294AR296DM298AR300AG302VS304IP306EI308MQ310AG312GW314TN316VN318IV3202017-3042Cre2,8202,8402,8602,8802,9002,9202,9402,960
    catgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattgaCTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTgtacttgatataggcattggacctatcactttgtccccgttaccacgcggacgaccttctaccgctaactGAGCTCGACCATGACGTACGTGCGTTACGATCGACGGGGAAAGGGCAGGACCCATGGGGCTCAGAGGGGGCTGGAGCCCAGGGTCCATACGAGGGTGGAGGTGGABmtIKflIKpnINheISphITliIXhoIPspXIAcc65IPaeR7IMN322YI324RN326LD328SE330TG332AM334VR336LL338ED340GD3422017-3042*1hybrid UTR3Crehomolog2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACGGGGTGAGTGGTGGAGACGATCAAGGTCTGTGGAGGGTTCGTGCGTCGTTACGTCGAGTTTTGCGAATCGGATCGGTGTGGGGGTGCCCTTTGTCGTCACTAATTGGAAATCGTTATTTGCTTTCAAATTGATTCGATATGATTGGGGTCCCAACCAGTTAAAGCACGGTCGGTBlpIPasIhybrid UTR33,1603,1803,2003,2203,2403,2603,2803,3003,320
    CACCCTGGAGCTAGCgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggGTGGGACCTCGATCGcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccApaIBclIBmtINheIPmeIXcmIPflMIPspOMIBGH polyadenylation sequencec-myc epitopePolyhi...e taghomologhybr...TR33,3403,3603,3803,4003,4203,4403,4603,4803,500
    aaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacBbsIClaISphIBspDIBGH polyadenylation sequence3,5203,5403,5603,5803,6003,6203,6403,660
    ttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagPvuIIBGH pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccKflINaeIXbaINgoMIVFRT3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaBarIMscIBsaXIPflFIPshAITth111IHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagcccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcgPstIEcoRIDraIIIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    cggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgNdeIAsiSIPflFIRsrIITth111IHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgcccaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggDraIIIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccAjuISacIIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagagcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctScaIHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    tttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtNaeIPsiINgoMIVSV40 pA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    aataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaattatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttBsmIBstZ17ISV40 pA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcaccgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgSapIBsaXIBspQIPvuII5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctcactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgagPciIpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    cgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgcgcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgpUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaaaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    caggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccapUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttpUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    gttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgccaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgAmp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    aatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaaBsaIFspIAmp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcaccaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgAmp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaatttScaIAmp (bla)7,0207,0407,0607,0807,1007,1207,1407,160
    agtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggatcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttccctAmp (bla)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggattattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctaSspISgrDIbla promoterAmp (bla)7,3607,3807,4007,4207,4407,4607,4807,5007,520
    cgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttgccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaaMfeI7,5407,5607,5807,6007,6207,6407,6607,6807,700
    agggttaggcgttttgcgctgcttcgtacgggccagatatacgctcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMluIBsiWI7,7207,740
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7744 bp5500600065007000500100015003' stemmutation siteopposite edited A1909-2007EKo437.2 (DNA)EKo438.4 (DNA)pNK018 (DNA)M13-48REV Genewiz (DNA)CMV,SpeI,NdeICMV promoter (with TetOs),A,CMV-Forward Genewiz (DNA),CspCITATA box,TetO,TetR binding sites,TetO,CMVF1 (DNA),ToFlpF (DNA),+8homolog,HindIII residue,T7 insert,XZp072.1 (DNA),PmeI,AflII,NotI,+2TEV leader UTR5,homolog,kozak,949-951,XZp388.1 (DNA),SwaI,+1EGFP-N Genewiz (DNA),M-SPOTIT-seq-F2 (DNA),Cit2 (DNA),BseRICit5 (DNA)mTur_2 (don't have?) (DNA),EKo433.1 (DNA)EGFP (encoding from LEAPER),952-1665f-egfp-2-qPCR (DNA)r-egfp-2-qPCR (DNA)f-egfp-1-qPCR (DNA),Cit7 (DNA),r-egfp-1-qPCR (DNA),+1homolog,+75' StopGo (2A; Loughran 2017),1675-1773,1774-1779,+35' stem,ADAR substrate,edited,bulged A -- edited?,+12loop3,loop MS2,bulged,bulge opposite the other A,+93' StopGo (2A; Loughran 2017),XZp388.4 (DNA)pUCoriR (DNA)pUC oriEKo152.2 (DNA)BsaIFspIAmp (bla)ScaISspIbla promoter,FlpInF1 (DNA),FlpInF (DNA),EKo152.4 (DNA),SgrDI,MfeI,BsiWI,MluI

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    7744

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