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LSM104 CMV-TO-AVPR2-(noExtraV2)-ADAR2(DD-E488Q-T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggpUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIAmp (bla)180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspIAmp (bla)360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp (bla)540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp (bla)720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoter8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactSgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIBsiWI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)1,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaNdeICMVCMV promoter (with TetOs)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggCspCISnaBICMVCMV promoter (with TetOs)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgBsaBIBsaBIBsmBIEsp3ISgrDICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatatgctcatggcctctaccacaagcgcCgtgcccggacatccagccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctatacgagtaccggagatggtgttcgcgGcacgggcctgtaggtClaIPmeIAflIIBspDIHindIIIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28LM30AS32TT34SA36VP38GH40P2153-4570AVPR2 (Tango)Signal/FlagCMV pro...TetOs)homologhomolog2,1202,1402,1602,1802,2002,2202,2402,260
    tctcttccaagtcttccctccaacagctcacaggagaggcctctggatacccgggacccacttcttgcaagagcggagctcgccttgctctccatcgttttcgttgccgtcgcgttgagcaatggtctggtgctggccgccctggcgcgacgaggccgccgaggccactgggcgcagagaaggttcagaagggaggttgtcgagtgtcctctccggagacctatgggccctgggtgaagaacgttctcgcctcgagcggaacgagaggtagcaaaagcaacggcagcgcaactcgttaccagaccacgaccggcgggaccgcgctgctccggcggctccggtgacccgcgBplIBplIKasINarISfiISfoISmaIStuIXmaITspMIS42LP44SL46PS48NS50SQ52ER54PL56DT58RD60PL62LA64RA66EL68AL70LS72IV74FV76AV78AL80SN82GL84VL86AA88LA90RR92GR94RG96HW98A2153-4570AVPR2 (Tango)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctattcatgtctttatcggccatctctgtcttgcagacctggccgtggcgctgtttcaggtgctccctcaactggcttggaaagccaccgatagattccgggggccagacgcactgtgtcgggccgtgaaatacctgcagatggtgggaatgtatgccagttcatatatgatactgataagtacagaaatagccggtagagacagaacgtctggaccggcaccgcgacaaagtccacgagggagttgaccgaacctttcggtggctatctaaggcccccggtctgcgtgacacagcccggcactttatggacgtctaccacccttacatacggtcaagtatatactatgaPluTIP100IH102VF104IG106HL108CL110AD112LA114VA116LF118QV120LP122QL124AW126KA128TD130RF132RG134PD136AL138CR140AV142KY144LQ146MV148GM150YA152SS154YM156IL1582153-4570AVPR2 (Tango)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgctatgaccttggacagacacagggctatctgtcgccccatgctggcgtataggcacggttccggagcccattggaacaggccagttctggttgcgtgggctttttccctgcttctgagcttgccccagttgtttattttcgcacagagaaatgtggagggcggatctggcgtgacgatactggaacctgtctgtgtcccgatagacagcggggtacgaccgcatatccgtgccaaggcctcgggtaaccttgtccggtcaagaccaacgcacccgaaaaagggacgaagactcgaacggggtcaacaaataaaagcgtgtctctttacacctcccgcctagaccgcacAM160TL162DR164HR166AI168CR170PM172LA174YR176HG178SG180AH182WN184RP186VL188VA190WA192FS194LL196LS198LP200QL202FI204FA206QR208NV210EG212GS214GV2162153-4570AVPR2 (Tango)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    accgattgctgggcttgcttcgctgagccatggggcagaaggacctacgtgacgtggattgctctcatggtgttcgttgctcccaccctggggatcgccgcatgccaggtcctgatattcagagaaatacacgcctccttggttcctgggccaagcgagcgccctggtggaagaatggctaacgacccgaacgaagcgactcggtaccccgtcttcctggatgcactgcacctaacgagagtaccacaagcaacgagggtgggacccctagcggcgtacggtccaggactataagtctctttatgtgcggaggaaccaaggacccggttcgctcgcgggaccaccttcttBlpIPasISphIBmgBITD218CW220AC222FA224EP226WG228RR230TY232VT234WI236AL238MV240FV242AP244TL246GI248AA250CQ252VL254IF256RE258IH260AS262LV264PG266PS268ER270PG272GR2742153-4570AVPR2 (Tango)2,8202,8402,8602,8802,9002,9202,9402,960
    gacggggtcgaaggactggctctccaggcgaaggcgcccacgtatcagctgcggtagctaagaccgtacgcatgacactggtcatcgtcgtcgtttacgttctttgctgggccccgttttttttggtgcagctctgggcggcctgggaccccgaagcacccctggaaggtgctccctgccccagcttcctgaccgagaggtccgcttccgcgggtgcatagtcgacgccatcgattctggcatgcgtactgtgaccagtagcagcagcaaatgcaagaaacgacccggggcaaaaaaaaccacgtcgagacccgccggaccctggggcttcgtggggaccttccacgaggApaIBsgIKasINarISfoIBsiWIPluTIPshAIPspOMIRR276GR278RT280GS282PG284EG286AH288VS290AA292VA294KT296VR298MT300LV302IV304VV306YV308LC310WA312PF314FL316VQ318LW320AA322WD324PE326AP328LE330GA332P2153-4570AVPR2 (Tango)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    attcgttctgctcatgcttctcgcttcactgaatagctgtaccaatccttggatttacgcctctttctcctccagtgttagctccgagctgcggagtcttctctgttgcgcccggggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctcctaagcaagacgagtacgaagagcgaagtgacttatcgacatggttaggaacctaaatgcggagaaagaggaggtcacaatcgaggctcgacgcctcagaagagacaacgcgggcccctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggaggBsgISmaIXcmIXmaIPflMITspMIF334VL336LM338LL340AS342LN344SC346TN348PW350IY352AS354FS356SS358VS360SE362LR364SL366LC368CA370RG372RT374PP376SL378GP380QD382ES384CT386TA388SS390S2153-4570AVPR2 (Tango)V2 tail3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCgaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGAgeIKpnIMscIEcoNIAcc65IBstEIIL392AK394DT396SS398GT400GA402LH404LD406QT408PS410RQ412PI414PS416EG418LQ420LH422LP424QV426LA428DA430VS432RL434VL436GK438FG440DL442TD444NF446SS448P2153-4570hADAR2 cat domainAVPR2 (Tango)V2 tailsho...er3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAH450AR452RK454VL456AG458VV460MT462TG464TD466VK468DA470KV472IS474VS476TG478TK480CI482NG484EY486MS488DR490GL492AL494ND496CH498AE500II502SR504RS506LL5082153-4570hADAR2 cat domain3,5203,5403,5603,5803,6003,6203,6403,660
    CAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTBclIBsrGIRF510LY512TQ514LE516LY518LN520NK522DD524QK526RS528IF530QK532SE534RG536GF538RL540KE542NV544QF546HL548YI550ST552SP554CG556DA558RI560FS562PH564EP5662153-4570hADAR2 cat domain3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACBcgIPfoIIL568EE570PA572DR574HP576NR578KA580RG582QL584RT586KI588ES590GQ592GT594IP596VR598SN600AS602IQ604AW606DG608VL610QG612ER614LL616TM618SC620SD622KI6242153-4570hADAR2 cat domain3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGBlpITliIXhoIBamHIPaeR7IAR626WN628VV630GI632QG634SL636LS638IF640VE642PI644YF646SS648II650LG652SL654YH656GD658HL660SR662AM664YQ666RI668SN670IE672DL674PP676LY678TL680NK682P2153-4570hADAR2 cat domain4,0404,0604,0804,1004,1204,1404,1604,1804,200
    TTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACMluIL684LS686GI688SN690AE692AR694QP696GK698AP700NF702SV704NW706TV708GD710SA712IE714VI716NA718TT720GK722DE724LG726RA728SR730LC732KH734AL736YC738RW740M2153-4570hADAR2 cat domain4,2204,2404,2604,2804,3004,3204,3404,360
    CGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGR742VH744GK746VP748SH750LL752RS754KI756TK758PN760VY762HE764SK766LA768AK770EY772QA774AK776AR778LF780TA782FI784KA786GL788GA790WV792EK794PT796EQ798D2153-4570hADAR2 cat domain4,3804,4004,4204,4404,4604,4804,5004,5204,540
    AGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcaApaIBclIPmeITliIXhoIPspXIPaeR7IPspOMI*1Q800FS802LT804P*8062153-4570BGH polyadenylation sequencec-myc epitopePolyhi...e taghADAR...main4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctcggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagaSphIBGH polyadenylation sequence4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgtacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcClaIBspDIBGH pA4,9204,9404,9604,9805,0005,0205,0405,060
    ctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctatgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataNaeINgoMIVFRT5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccBarIMscIXbaIBsmBIEsp3IPshAIHygromycinFRT5,2605,2805,3005,3205,3405,3605,3805,4005,420
    taaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgaEcoRIBstAPIDraIIIHygromycin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatgcgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacNdeIPvuIAsiSIRsrIIHygromycin5,6205,6405,6605,6805,7005,7205,7405,760
    gacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaaBstAPIDraIIIHygromycin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttgggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaaAjuISacIIHygromycin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttScaIHygromycin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaataatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatNaeIPfoIPsiINgoMIVSV40 pA6,3206,3406,3606,3806,4006,4206,4406,460
    gcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtBsmIBstZ17ISV40 pA6,4806,5006,5206,5406,5606,5806,6006,6206,640
    caacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctctgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgaga6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccPciISapIBspQIpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggpUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    ttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactgaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgacpUC ori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttpUC ori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttpUC ori7,5407,5607,580
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7593 bp550060006500700050010001500HygromycinCMV promoter (with TetOs)CMVASV40 pAbla promoterAmp (bla)pUC oriEKo152.2 (DNA)EKbF (DNA)oLSM0299 (DNA)oLSM0301 (DNA)XZp025.2 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)AhdI,BsaIFspI,AclIPvuIScaI,BcgIAclIXmnISspISgrDIFlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),MfeIEKo152.4 (DNA),EKo279.1 (DNA),EKo272.1 (DNA),BsiWI,+1SpeIEK_CMVFa (DNA)EK_CMVFb (DNA)EK_CMVFc (DNA),NdeIEK_CMVFd (DNA),SnaBIEK_CMVFe (DNA)CspCINdeIBstAPIDraIIIAjuISacIIHyg_F (DNA)ScaIPfoI,NgoMIV,NaeISV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiIBsmI,BstZ17IM13R Genewiz (DNA)pUCori5F (DNA),BspQI,SapIPciI

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    7593

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