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pAAV-cFos-ER T2-CreERT2-ds-p2A-No WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaagctttcctttaggaacagggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcgaaaggaaatccttgtcMluINotISbfIL-ITRFos Tru...ron 1)20406080100120140160
    aggcttcgagcctttaaggctgcgtacttgcttctcctaataccagagactcaaaaaaaaaaaaaaagttccagattgctggacaatgacccgggtctcatcccttgaccctgggaaccgggtccacattgaatcaggtgcgaatgttcgctcgccttctctgcctttcccgccttccgaagctcggaaattccgacgcatgaacgaagaggattatggtctctgagtttttttttttttttcaaggtctaacgacctgttactgggcccagagtagggaactgggacccttggcccaggtgtaacttagtccacgcttacaagcgagcggaagagacggaaagggcggaPasIBstXIPshAIFos Truncation (after Intron 1)180200220240260280300320340
    cccctcccccggccgcggccccggttccccccctgcgctgcaccctcagagttggctgcagccggcgagctgttcccgtcaatccctccctcctttacacaggatgtccatattaggacatctgcgtcagcaggtttccacggccggtccctgttgttctgggggggggaccatcggggagggggccggcgccggggccaagggggggacgcgacgtgggagtctcaaccgacgtcggccgctcgacaagggcagttagggagggaggaaatgtgtcctacaggtataatcctgtagacgcagtcgtccaaaggtgccggccagggacaacaagaccccccccctggtagBsgISacIIFos Truncation (after Intron 1)360380400420440460480500520
    tccgaaatcctacacgcggaaggtctaggagaccccctaagatcccaaatgtgaacactcataggtgaaagatgtatgccaagacgggggttgaaagcctggggcgtagagttgacgacagagcgcccgcagagggccttggggcgcgcttccccccccttccagttccgcccagaggctttaggatgtgcgccttccagatcctctgggggattctagggtttacacttgtgagtatccactttctacatacggttctgcccccaactttcggaccccgcatctcaactgctgtctcgcgggcgtctcccggaaccccgcgcgaaggggggggaaggtcaaggcgggtcFos Truncation (after Intron 1)540560580600620640660680700
    tgacgtaggaagtccatccattcacagcgcttctataaaggcgccagctgaggcgcctactactccaaccgcgactgcagcgagcaactgagaagactggatagagccggcggttccgcgaacgagcagtgaccgcgctcccacccagctctgctctgcagctcccaccagtgtcactgcatccttcaggtaggtaagtgtcgcgaagatatttccgcggtcgactccgcggatgatgaggttggcgctgacgtcgctcgttgactcttctgacctatctcggccgccaaggcgcttgctcgtcactggcgcgagggtgggtcgagacgagacgtcgagggtggtcacagAccIAfeIFos Truncation (after Intron 1)720740760780800820840860
    tacccctggaccccttgccgggctttccccaaacttcgaccatgatgttctcgggtttcaacgccgactacgaggcgtcatcctcccgctgcagtagcgcctccccggccggggacagcctttcctactaccattccccagccgactccttctccagcatgggctctcctgtcaaatggggacctggggaacggcccgaaaggggtttgaagctggtactacaagagcccaaagttgcggctgatgctccgcagtaggagggcgacgtcatcgcggaggggccggcccctgtcggaaaggatgatggtaaggggtcggctgaggaagaggtcgtacccgagaggacagttFos Truncation (after Intron 1)8809009209409609801,0001,0201,040
    cacacaggtgagtttggctttgtgtagccgccaggtccgcgctgagggtcgccgtggaggagacactggggtgtgactcgcaggggcgggggggtcttcctttttcgctctggagggagactggcgcggtcagagcagccttagcctgggaacccaggacttgtctgagcgcgtggtgtgtccactcaaaccgaaacacatcggcggtccaggcgcgactcccagcggcacctcctctgtgaccccacactgagcgtccccgcccccccagaaggaaaaagcgagacctccctctgaccgcgccagtctcgtcggaatcggacccttgggtcctgaacagactcgcgcacFos Truncation (after Intron 1)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cacacttgtcatagtaagacttagtgaccccttcccgcgcggcaggtttattctgagtggcctgcctgcattcttctctcggccgacttgtttctgagatcagccggggccaacaagtctcgagcaaagagtcgctaactagagtttgggaggcggcaaaccgcggcaatcccccgtgtgaacagtatcattctgaatcactggggaagggcgcgccgtccaaataagactcaccggacggacgtaagaagagagccggctgaacaaagactctagtcggccccggttgttcagagctcgtttctcagcgattgatctcaaaccctccgccgtttggcgccgttagggggSacIINmeAIIIFos Truncation (after Intron 1)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ctcccggggcagcctggagcagggaggagggaggagggaggagggtgctgcgggcgggtgtgtaaggcagtttcattgataaaaagcgagttcattctggagactccggagcagcgcctgcgtcagcgcagacgtcagggatatttataacaaaccccctttcgagcgagtgatggagggccccgtcggacctcgtccctcctccctcctccctcctcccacgacgcccgcccacacattccgtcaaagtaactatttttcgctcaagtaagacctctgaggcctcgtcgcggacgcagtcgcgtctgcagtccctataaatattgtttgggggaaagctcgctcactacZraIAatIIBspEIEcoNIFos Truncation (after Intron 1)1,4201,4401,4601,4801,5001,5201,5401,560
    ccgaagggataacgggaacgcagcagtaggatggaggagaaaggctgcgctgcggaattcaagggaggatattgggagagcttttatctccgatgaggtgcatacaggaagacataagcagtctctgaccggaatgcttctctctccctgcttcatgcgacactagggccacttgggcttccctattgcccttgcgtcgtcatcctacctcctctttccgacgcgacgccttaagttccctcctataaccctctcgaaaatagaggctactccacgtatgtccttctgtattcgtcagagactggccttacgaagagagagggacgaagtacgctgtgatcccggtgaacEcoRIFos Truncation (after Intron 1)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctccacctgtgtctggaacctcctcgctcacctccgctttcctctttttgttttgtttcagtaaggatcctctagagtcgaggccaccatggctggagacatgagagctgccaacctttggccaagcccgctcatgatcaaacgctctaagaagaacagcctggccttgtccctggaggtggacacagaccttggaggagcgagtggaggcgaaaggagaaaaacaaaacaaagtcattcctaggagatctcagctccggtggtaccgacctctgtactctcgacggttggaaaccggttcgggcgagtactagtttgcgagattcttcttgtcggaccggaacagggacBamHIER T2ER T2-Cre-ER T2Fos Truncation (after Intron 1)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    acggccgaccagatggtcagtgccttgttggatgctgagccccccatactctattccgagtatgatcctaccagacccttcagtgaagcttcgatgatgggcttactgaccaacctggcagacagggagctggttcacatgatcaactgggcgaagagggtgccaggctttgtggtgccggctggtctaccagtcacggaacaacctacgactcggggggtatgagataaggctcatactaggatggtctgggaagtcacttcgaagctactacccgaatgactggttggaccgtctgtccctcgaccaagtgtactagttgacccgcttctcccacggtccgaaacaccER T2ER T2-Cre-ER T21,9401,9601,9802,0002,0202,0402,0602,0802,100
    atttgaccctccatgatcaggtccaccttctagaatgtgcctggctagagatcctgatgattggtctcgtctggcgctccatggagcacccagtgaagctactgtttgctcctaacttgctcttggacaggaaccagggaaaatgtgtagagggcatggtggagatcttcgacattaaactgggaggtactagtccaggtggaagatcttacacggaccgatctctaggactactaaccagagcagaccgcgaggtacctcgtgggtcacttcgatgacaaacgaggattgaacgagaacctgtccttggtcccttttacacatctcccgtaccacctctagaagctgtaER T2ER T2-Cre-ER T22,1202,1402,1602,1802,2002,2202,2402,260
    gctgctggctacatcatctcggttccgcatgatgaatctgcagggagaggagtttgtgtgcctcaaatctattattttgcttaattctggagtgtacacatttctgtccagcaccctgaagtctctggaagagaaggaccatatccaccgagtcctggacaagatcacagacactcgacgaccgatgtagtagagccaaggcgtactacttagacgtccctctcctcaaacacacggagtttagataataaaacgaattaagacctcacatgtgtaaagacaggtcgtgggacttcagagaccttctcttcctggtataggtggctcaggacctgttctagtgtctgtgaER T2ER T2-Cre-ER T22,2802,3002,3202,3402,3602,3802,4002,4202,440
    ttgatccacctgatggccaaggcaggcctgaccctgcagcagcagcaccagcggctggcccagctcctcctcatcctctcccacatcaggcacatgagtaacaaaggcatggagcatctgtacagcatgaagtgcaagaacgtggtgcccctctatgacctgctgctggaggcggaactaggtggactaccggttccgtccggactgggacgtcgtcgtcgtggtcgccgaccgggtcgaggaggagtaggagagggtgtagtccgtgtactcattgtttccgtacctcgtagacatgtcgtacttcacgttcttgcaccacggggagatactggacgacgacctccgccStuIER T2ER T2-Cre-ER T22,4602,4802,5002,5202,5402,5602,5802,6002,620
    cggacgcccaccgcctacatgcgcccactagccgtggaggggcatccgtggaggagacggaccaaagccacttggccactgcgggctctacttcatcgcattccttgcaaaagtattacatcacgggggaggcagagggtttccctgccacagctgtcgacaatttactgaccgtgcctgcgggtggcggatgtacgcgggtgatcggcacctccccgtaggcacctcctctgcctggtttcggtgaaccggtgacgcccgagatgaagtagcgtaaggaacgttttcataatgtagtgccccctccgtctcccaaagggacggtgtcgacagctgttaaatgactggcaAccISalIER T2-Cre-ER T2ER T2Cre2,6402,6602,6802,7002,7202,7402,7602,7802,800
    acaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttccctgtggttttaaacggacgtaatggccagctacgttgctcactactccaagcgttcttggactacctgtacaagtccctagcggtccgcaaaagactcgtatggaccttttacgaagacaggcaaacggccagcacccgccgtaccacgttcaacttattggcctttaccaaagggAgeIPciIER T2-Cre-ER T2Cre2,8202,8402,8602,8802,9002,9202,9402,960
    gcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaacgtcttggacttctacaagcgctaatagaagatatagaagtccgcgcgccagaccgtcatttttgataggtcgttgtaaacccggtcgatttgtacgaagtagcagccaggcccgacggtgctggttcactgtcgttacgacaaagtgaccaatacgccgcctaggcttttctttNruIXmnIBamHIRsrIIER T2-Cre-ER T2Cre2,9803,0003,0203,0403,0603,0803,1003,1203,140
    acgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttgcaactacggccacttgcacgttttgtccgagatcgcaagcttgcgtgactaaagctggtccaagcaagtgagtaccttttatcgctagcgacggtcctatatgcattagaccgtaaagacccctaacgaatattgtgggacaatgcatatcggctttaacggtcctagtcccaPvuIAsiSIBstBISexAISnaBISnaBIER T2-Cre-ER T2Cre3,1603,1803,2003,2203,2403,2603,2803,3003,320
    taaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgcatttctatagagtgcatgactgccaccctcttacaattaggtataaccgtcttgcttttgcgaccaatcgtggcgtccacatctcttccgtgaatcggacccccattgatttgaccagctcgctacctaaaggcagagaccacatcgactactaggcttattgatggacaaaacgEcoRVPaqCIER T2-Cre-ER T2Cre3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatagcccagtcttttttaccacaacggcgcggtagacggtggtcggtcgatagttgagcgcgggaccttccctaaaaacttcgttgagtagctaactaaatgccgcgattcctactgagaccagtctctatggaccggaccagacctgtgtcacgggcacagcctcggcgcgctctatClaIBspDIPshAIER T2-Cre-ER T2Cre3,5203,5403,5603,5803,6003,6203,6403,660
    tggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatctcgagccatctgctggagacatgagagctgccaacctttgaccgggcgcgacctcaaagttatggcctctagtacgttcgaccaccgacctggttacatttataacagtacttgatataggcattggacctatcactttgtccccgttaccacgcggacgaccttctaccgctagagctcggtagacgacctctgtactctcgacggttggaaacBsaBIER T2-Cre-ER T2CreER T23,6803,7003,7203,7403,7603,7803,8003,8203,840
    gccaagcccgctcatgatcaaacgctctaagaagaacagcctggccttgtccctgacggccgaccagatggtcagtgccttgttggatgctgagccccccatactctattccgagtatgatcctaccagacccttcagtgaagcttcgatgatgggcttactgaccaacctggcacggttcgggcgagtactagtttgcgagattcttcttgtcggaccggaacagggactgccggctggtctaccagtcacggaacaacctacgactcggggggtatgagataaggctcatactaggatggtctgggaagtcacttcgaagctactacccgaatgactggttggaccgtER T2-Cre-ER T2ER T23,8603,8803,9003,9203,9403,9603,9804,0004,020
    gacagggagctggttcacatgatcaactgggcgaagagggtgccaggctttgtggatttgaccctccatgatcaggtccaccttctagaatgtgcctggctagagatcctgatgattggtctcgtctggcgctccatggagcacccagtgaagctactgtttgctcctaacttgcctgtccctcgaccaagtgtactagttgacccgcttctcccacggtccgaaacacctaaactgggaggtactagtccaggtggaagatcttacacggaccgatctctaggactactaaccagagcagaccgcgaggtacctcgtgggtcacttcgatgacaaacgaggattgaacgER T2-Cre-ER T2ER T24,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcttggacaggaaccagggaaaatgtgtagagggcatggtggagatcttcgacatgctgctggctacatcatctcggttccgcatgatgaatctgcagggagaggagtttgtgtgcctcaaatctattattttgcttaattctggagtgtacacatttctgtccagcaccctgaaagaacctgtccttggtcccttttacacatctcccgtaccacctctagaagctgtacgacgaccgatgtagtagagccaaggcgtactacttagacgtccctctcctcaaacacacggagtttagataataaaacgaattaagacctcacatgtgtaaagacaggtcgtgggacttER T2-Cre-ER T2ER T24,2204,2404,2604,2804,3004,3204,3404,360
    gtctctggaagagaaggaccatatccaccgagtcctggacaagatcacagacactttgatccacctgatggccaaggcaggcctgaccctgcagcagcagcaccagcggctggcccagctcctcctcatcctctcccacatcaggcacatgagtaacaaaggcatggagcatctgcagagaccttctcttcctggtataggtggctcaggacctgttctagtgtctgtgaaactaggtggactaccggttccgtccggactgggacgtcgtcgtcgtggtcgccgaccgggtcgaggaggagtaggagagggtgtagtccgtgtactcattgtttccgtacctcgtagacStuIER T2-Cre-ER T2ER T24,3804,4004,4204,4404,4604,4804,5004,5204,540
    tacagcatgaagtgcaagaacgtggtgcccctctatgacctgctgctggaggcggcggacgcccaccgcctacatgcgcccactagccgtggaggggcatccgtggaggagacggaccaaagccacttggccactgcgggctctacttcatcgcattccttgcaaaagtattacaatgtcgtacttcacgttcttgcaccacggggagatactggacgacgacctccgccgcctgcgggtggcggatgtacgcgggtgatcggcacctccccgtaggcacctcctctgcctggtttcggtgaaccggtgacgcccgagatgaagtagcgtaaggaacgttttcataatgtER T2-Cre-ER T2ER T24,5604,5804,6004,6204,6404,6604,6804,7004,720
    tcacgggggaggcagagggtttccctgccacagctagccatggcttcccgccggaggtggaggagcaggatgatggcacgctgcccatgtcttgtgcccaggagagcgggatggaccgtcaccctgcagcctgtgcttctgctaggatcaatgtgggcgcgcccggctccggagcagtgccccctccgtctcccaaagggacggtgtcgatcggtaccgaagggcggcctccacctcctcgtcctactaccgtgcgacgggtacagaacacgggtcctctcgccctacctggcagtgggacgtcggacacgaagacgatcctagttacacccgcgcgggccgaggcctcgAscIBmtINheIBspEIMODC Destab PESTER T2-Cre-ER T2ER T24,7404,7604,7804,8004,8204,8404,8604,8804,900
    cacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcccgtggtgtaagaattcgatatcaagcttatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgtgcttgaagagagacaatttcgttcgtcctctgcaccttcttttggggccagggcaccacattcttaagctatagttcgaatagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcaAfeIBplIBplIClaIBmgBIBspDIEcoRIEcoRVhGH polyAp2A4,9204,9404,9604,9805,0005,0205,0405,060
    gcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatctcgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaCspCIhGH polyA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagaBsgINsiISphIhGH polyA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    acccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtAloINotIPmlIBstXIRsrIIBstEIIR-ITRhGH polyA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgSbfIR-ITRf1 Origin5,6205,6405,6605,6805,7005,7205,7405,760
    gggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaacccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggtttf1 Origin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggtttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccAloIf1 Origin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacga6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctZraIAatII6,3206,3406,3606,3806,4006,4206,4406,460
    aatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaBciVIAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagXmnIAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgtScaIAmpR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    acgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactactgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgFspIPvuIAmpR7,0207,0407,0607,0807,1007,1207,1407,160
    ttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcAseINmeAIIIAmpR7,1807,2007,2207,2407,2607,2807,3007,3207,340
    tatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttcAmpR7,3607,3807,4007,4207,4407,4607,4807,5007,520
    atcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagpUC ori7,5407,5607,5807,6007,6207,6407,6607,6807,700
    aagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagpUC ori7,7207,7407,7607,7807,8007,8207,8407,860
    gtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggaccacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgpUC ori7,8807,9007,9207,9407,9607,9808,0008,0208,040
    aggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggccttccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaBciVIpUC ori8,0608,0808,1008,1208,1408,1608,1808,2008,220
    ttttacggttcctggccttttgctggccttttgctcacatgtaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIpUC ori8,2408,260
    • Plasmid
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    8267 bp60006500700075008000500100015002000ER T2IRES

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    8267