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APL0031 SFFV-AVPR2-ADAR2dd (FLP-IN) from EK0334/XZ030
  • Sequence Map
    Editing disabled on read-only sequence.
    gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcacagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagt*W286HK284IL282SA280GI278E145-1005Amp (bla)20406080100120140160
    gcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtgcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcacAhdIBsaIA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220LL218P145-1005Amp (bla)180200220240260280300320340
    gtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtcaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaAclIFspIG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162DL160RT158145-1005Amp (bla)360380400420440460480500520
    tacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtPvuIScaIVH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104YE102VL100D145-1005Amp (bla)540560580600620640660680700
    ttctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgAclIBcgIXmnIN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44GV42RA40G145-1005Amp (bla)720740760780800820840860880
    ccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtaSspIL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M145-1005Amp (bla)bla promoter9009209409609801,0001,0201,0401,060
    atttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtaaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccZraIAatIIBglIISgrDIbla promoter1,0801,1001,1201,1401,1601,1801,2001,2201,240
    tcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaagctagctgcagtaacgccattattgcaaggcatggaaaaataagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaataccgttcgatcgacgtcattgcggtaataacgttccgtacctttttatBmtIMfeINheISFFV1,2601,2801,3001,3201,3401,3601,3801,4001,420
    ccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggcccgaggccaagagcagatggtccccagatatggcccggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggcgtcaaagccggggccgggctccggttctcgtctaccaggggtctataccgggEcoRVBstEIISFFV1,4401,4601,4801,5001,5201,5401,5601,5801,600
    aaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttctgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctcttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagacgaagggctcgagatattttctcgagtgttggggagtgagccgcgcggtcaggagAscIAflIIBbvCIBpu10IBssHIIBssHIISFFV1,6201,6401,6601,6801,7001,7201,7401,7601,780
    cgacagactgagtcgcccgggaagcttcgccaccatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatatgctcatggcctctaccacaagcgcCgtgcccggacatccatctcttccaagtcttccctccgctgtctgactcagcgggcccttcgaagcggtggtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctatacgagtaccggagatggtgttcgcgGcacgggcctgtaggtagagaaggttcagaagggaggBpu10IHindIIIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28LM30AS32TT34SA36VP38GH40PS42LP44SL46PS481815-4325AVPR2 (Tango)Signal/FlagSFFVhomolog1,8001,8201,8401,8601,8801,9001,9201,940
    aacagctcacaggagaggcctctggatacccgggacccacttcttgcaagagcggagctcgccttgctctccatcgttttcgttgccgtcgcgttgagcaatggtctggtgctggccgccctggcgcgacgaggccgccgaggccactgggcgcctattcatgtctttatcggccatcttgtcgagtgtcctctccggagacctatgggccctgggtgaagaacgttctcgcctcgagcggaacgagaggtagcaaaagcaacggcagcgcaactcgttaccagaccacgaccggcgggaccgcgctgctccggcggctccggtgacccgcggataagtacagaaatagccggtagBplIBplIKasINarISfiISfoIStuIPluTINS50SQ52ER54PL56DT58RD60PL62LA64RA66EL68AL70LS72IV74FV76AV78AL80SN82GL84VL86AA88LA90RR92GR94RG96HW98AP100IH102VF104IG106H1815-4325AVPR2 (Tango)1,9601,9802,0002,0202,0402,0602,0802,1002,120
    tctgtcttgcagacctggccgtggcgctgtttcaggtgctccctcaactggcttggaaagccaccgatagattccgggggccagacgcactgtgtcgggccgtgaaatacctgcagatggtgggaatgtatgccagttcatatatgatacttgctatgaccttggacagacacagggcagacagaacgtctggaccggcaccgcgacaaagtccacgagggagttgaccgaacctttcggtggctatctaaggcccccggtctgcgtgacacagcccggcactttatggacgtctaccacccttacatacggtcaagtatatactatgaacgatactggaacctgtctgtgtcccgL108CL110AD112LA114VA116LF118QV120LP122QL124AW126KA128TD130RF132RG134PD136AL138CR140AV142KY144LQ146MV148GM150YA152SS154YM156IL158AM160TL162DR164HR166A1815-4325AVPR2 (Tango)2,1402,1602,1802,2002,2202,2402,2602,2802,300
    tatctgtcgccccatgctggcgtataggcacggttccggagcccattggaacaggccagttctggttgcgtgggctttttccctgcttctgagcttgccccagttgtttattttcgcacagagaaatgtggagggcggatctggcgtgaccgattgctgggcttgcttcgctgagccaatagacagcggggtacgaccgcatatccgtgccaaggcctcgggtaaccttgtccggtcaagaccaacgcacccgaaaaagggacgaagactcgaacggggtcaacaaataaaagcgtgtctctttacacctcccgcctagaccgcactggctaacgacccgaacgaagcgactcggtBlpINcoII168CR170PM172LA174YR176HG178SG180AH182WN184RP186VL188VA190WA192FS194LL196LS198LP200QL202FI204FA206QR208NV210EG212GS214GV216TD218CW220AC222FA224EP2261815-4325AVPR2 (Tango)2,3202,3402,3602,3802,4002,4202,4402,4602,480
    tggggcagaaggacctacgtgacgtggattgctctcatggtgttcgttgctcccaccctggggatcgccgcatgccaggtcctgatattcagagaaatacacgcctccttggttcctgggccaagcgagcgccctggtggaagaagacggggtcgaaggactggctctccaggcgaagaccccgtcttcctggatgcactgcacctaacgagagtaccacaagcaacgagggtgggacccctagcggcgtacggtccaggactataagtctctttatgtgcggaggaaccaaggacccggttcgctcgcgggaccaccttcttctgccccagcttcctgaccgagaggtccgcttcPasISphIBmgBIWG228RR230TY232VT234WI236AL238MV240FV242AP244TL246GI248AA250CQ252VL254IF256RE258IH260AS262LV264PG266PS268ER270PG272GR274RR276GR278RT280GS282PG284E1815-4325AVPR2 (Tango)2,5002,5202,5402,5602,5802,6002,6202,6402,660
    gcgcccacgtatcagctgcggtagctaagaccgtacgcatgacactggtcatcgtcgtcgtttacgttctttgctgggccccgttttttttggtgcagctctgggcggcctgggaccccgaagcacccctggaaggtgctccattcgttctgctcatgcttctcgcttcactgaatagcgcgggtgcatagtcgacgccatcgattctggcatgcgtactgtgaccagtagcagcagcaaatgcaagaaacgacccggggcaaaaaaaaccacgtcgagacccgccggaccctggggcttcgtggggaccttccacgaggtaagcaagacgagtacgaagagcgaagtgacttatcApaIKasINarISfoIBsiWIPluTIPshAIPspOMIG286AH288VS290AA292VA294KT296VR298MT300LV302IV304VV306YV308LC310WA312PF314FL316VQ318LW320AA322WD324PE326AP328LE330GA332PF334VL336LM338LL340AS342LN344S1815-4325AVPR2 (Tango)2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ctgtaccaatccttggatttacgcctctttctcctccagtgttagctccgagctgcggagtcttctctgttgcgcccggggcaggacccccccaagcctgggtccacaggacgaatcctgcactaccgcctccagttccctggccaaagacacctcttctatcgataccggtggacgcgacatggttaggaacctaaatgcggagaaagaggaggtcacaatcgaggctcgacgcctcagaagagacaacgcgggccccgtcctgggggggttcggacccaggtgtcctgcttaggacgtgatggcggaggtcaagggaccggtttctgtggagaagatagctatggccacctgcgAgeIBstXIPflMIC346TN348PW350IY352AS354FS356SS358VS360SE362LR364SL366LC368CA370RG372RT374PP376SL378GP380QD382ES384CT386TA388SS390SL392AK394DT396SS398ID400TG402GR4041815-4325AVPR2 (Tango)2,8602,8802,9002,9202,9402,9602,9803,0003,020
    accccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGAgeIKpnIXcmIEcoNIAcc65ITP406PS408LG410PQ412DE414SC416TT418AS420SS422LA424KD426TS428SG430TG432AL434HL436DQ438TP440SR442QP444IP446SE448GL450QL452HL454PQ456VL458AD460AV462S1815-4325hADAR2 cat domainAVPR2 (Tango)V2 tailhomologous3,0403,0603,0803,1003,1203,1403,1603,1803,200
    GCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGABstEIIR464LV466LG468KF470GD472LT474DN476FS478SP480HA482RR484KV486LA488GV490VM492TT494GT496DV498KD500AK502VI504SV506ST508GT510KC512IN514GE516YM518SD520RG522L1815-4325hADAR2 cat domain3,2203,2403,2603,2803,3003,3203,3403,3603,380
    TGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGBclIBsrGIA524LN526DC528HA530EI532IS534RR536SL538LR540FL542YT544QL546EL548YL550NN552KD554DQ556KR558SI560FQ562KS564ER566GG568FR570LK572EN574VQ576FH578LY580IS5821815-4325hADAR2 cat domain3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGBcgIPfoITS584PC586GD588AR590IF592SP594HE596PI598LE600EP602AD604RH606PN608RK610AR612GQ614LR616TK618IE620SG622QG624TI626PV628RS630NA632SI634QA636WD638GV640L1815-4325hADAR2 cat domain3,5803,6003,6203,6403,6603,6803,7003,720
    AAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAATTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTBlpITliIXhoIBamHIPaeR7IQ642GE644RL646LT648MS650CS652DK654IA656RW658NV660VG662IQ664GS666LL668SI670FV672EP674IY676FS678SI680IL682GS684LY686HG688DH690LS692RA694MY696QR698IS700N1815-4325hADAR2 cat domain3,7403,7603,7803,8003,8203,8403,8603,8803,900
    CATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGI702ED704LP706PL708YT710LN712KP714LL716SG718IS720NA722EA724RQ726PG728KA730PN732FS734VN736WT738VG740DS742AI744EV746IN748AT750TG752KD754EL756GR758AS7601815-4325hADAR2 cat domain3,9203,9403,9603,9804,0004,0204,0404,0604,080
    CGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCMluIRL762CK764HA766LY768CR770WM772RV774HG776KV778PS780HL782LR784SK786IT788KP790NV792YH794ES796KL798AA800KE802YQ804AA806KA808RL810FT812AF814IK816AG818L1815-4325hADAR2 cat domain4,1004,1204,1404,1604,1804,2004,2204,2404,260
    GGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgacgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactgcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggApaIBclIPmeITliIXhoIPspXIPaeR7IPspOMIG820AW822VE824KP826TE828QD830QF832SL834TP836*1815-4325hADAR2 cat domainc-myc epitopeBGH polya...sequencePolyhi...e tag4,2804,3004,3204,3404,3604,3804,4004,4204,440
    agccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcagtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtcBGH polyadenylation sequence4,4604,4804,5004,5204,5404,5604,5804,6004,620
    gcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtSphIBglIIBGH pABGH...ce4,6404,6604,6804,7004,7204,7404,7604,7804,800
    cttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaNaeINgoMIV4,8204,8404,8604,8804,9004,9204,9404,9604,980
    cctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcaBarIXbaIZraIAatIIBsmBIEsp3IPshAIHygromycinFRT5,0005,0205,0405,0605,0805,1005,1205,1405,160
    ggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgccctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacgEcoRIBstAPIDraIIIHygromycin5,1805,2005,2205,2405,2605,2805,3005,3205,340
    ctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcagactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtNcoINdeIPvuIAsiSIRsrIIHygromycin5,3605,3805,4005,4205,4405,4605,4805,500
    aactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagBstAPIDraIIIHygromycin5,5205,5405,5605,5805,6005,6205,6405,6605,680
    ggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcacccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgtAjuISacIIHygromycin5,7005,7205,7405,7605,7805,8005,8205,8405,860
    atttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggataaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttcctScaIHygromycin5,8805,9005,9205,9405,9605,9806,0006,0206,040
    atagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagctatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgNaeIPfoIPsiINgoMIVSV40 pA6,0606,0806,1006,1206,1406,1606,1806,2006,220
    atcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtBsmIBstZ17ISV40 pA6,2406,2606,2806,3006,3206,3406,3606,3806,400
    tacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaagSapIBspQI6,4206,4406,4606,4806,5006,5206,5406,5606,580
    ctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatgagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtaPciIpUC ori6,6006,6206,6406,6606,6806,7006,7206,7406,760
    aggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccpUC ori6,7806,8006,8206,8406,8606,8806,9006,9206,940
    cgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaagcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattpUC ori6,9606,9807,0007,0207,0407,0607,0807,1007,120
    caggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccapUC ori7,1407,1607,1807,2007,2207,2407,2607,280
    ttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgcccpUC ori7,3007,3207,3407,360
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7372 bp550060006500700050010001500Signal/Flag1815-4325145-1005EKo152.2 (DNA)EKo271.2 (DNA)oAPL0101 (DNA)XZp185.3 (DNA)XZp025.2 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)AhdI,BsaIFspI,AclIAmp (bla),PvuIScaI,BcgIAclIXmnIbla promoter,SspIZraI,SgrDI,AatII,BglIIFlpInF1 (DNA)FlpInF (DNA),EKo285.1 (DNA),MfeImissing in pSLQ2349,missing in pSLQ2349,NheI,BmtIA->T from pSLQ2349SFFV,EcoRV,BstEIIG->A from pSLQ2349BbvCI,Bpu10I,AflIIEK_SFFV_F2 (DNA),BssHII,AscI,BssHIIHindIIIKozak,oLSM0133-temp (DNA),XZp037.1 (DNA),Bpu10IHygromycinBstAPIDraIIIAjuISacIIHyg_F (DNA)ScaIPfoI,NgoMIV,NaeISV40 pA,SV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiI,BsmIBstZ17IM13R Genewiz (DNA)BspQI,SapIpUCori5F (DNA),PciIpUC_oriR_Fv2 (DNA),pUCoriR (DNA)pUC ori

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