Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
working...
Advanced Settings
pCDF_duet-Helper_v1
  • Sequence Map
Editing disabled on read-only sequence.
GGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACCATGGGCAGCAGCCATCACCATCATCACCACAGCCAGGATCCAAATGAACGATATAGTATAGTCCTTTTTTCTGAAGAATATGACAGGACTGATATAGAACTTTATCCCCTTAACACTCGCCTATTGTTAAGGGGACATCTTTATTAAAACAAATTGAAATTATTCCTCTATATGGTACCCGTCGTCGGTAGTGGTAGTAGTGGTGTCGGTCCTAGGTTTACTTGCTATATCATATCAGGAAAAAAGACTTCTTATACTGTCCTGACTATATCTTGAAATANcoIBamHIExtracted region from T4 genometrans_clamp_F20406080100120140160
CTTTATTATTAGCAATTCCCATACGAGTCAAAGTTTCTTTTACTTTCAGAAAATCTTCAGGTTTTTTAAGAGTAATTTCAATCATTTTACCATTCCAATGCTAGTTTTTTGAGTTGTTTCTGTTCTTTTACGTTCTTAGTCACTTCTTTCAAAAAATCATCCGTGACTAAACCTTGAAATAATAATCGTTAAGGGTATGCTCAGTTTCAAAGAAAATGAAAGTCTTTTAGAAGTCCAAAAAATTCTCATTAAAGTTAGTAAAATGGTAAGGTTACGATCAAAAAACTCAACAAAGACAAGAAAATGCAAGAATCAGTGAAGAAAGTTTTTTAGTAGGCACTGATTTGGAAExtracted region from T4 genome180200220240260280300320340
TTAGTTCTTTTAATACTAAAGGAAGTTTTCCATTTTTAGTAAGAATTGATTTATAGTTAATTGCATCATTTGTATTAACTTGATACCGCTTAGCAAGTAACTTAATAATCAATACTTCGGTGGAATCTTCAACCAGTTTTGCCCATTTACCATATCTTTTACCACGAGGAACTGCAATCAAGAAAATTATGATTTCCTTCAAAAGGTAAAAATCATTCTTAACTAAATATCAATTAACGTAGTAAACATAATTGAACTATGGCGAATCGTTCATTGAATTATTAGTTATGAAGCCACCTTAGAAGTTGGTCAAAACGGGTAAATGGTATAGAAAATGGTGCTCCTTGACGBssSIBssSαIExtracted region from T4 genome360380400420440460480500520
AGCCATTAGATAATTAAAATGAGCTTCATCACTTAAGCCTGATCCAATTAAATTCATAGCATATACAGCTGGCATACACTCTGGAAATTGTGATAATGCATTTTCAACCATGAATTTTGAATAATCTTTTTGAGCAATAGAGCATTTAGTTTTATTATTAATAGCTCCAATTATTTCGGTAATCTATTAATTTTACTCGAAGTAGTGAATTCGGACTAGGTTAATTTAAGTATCGTATATGTCGACCGTATGTGAGACCTTTAACACTATTACGTAAAAGTTGGTACTTAAAACTTATTAGAAAAACTCGTTATCTCGTAAATCAAAATAATAATTATCGAGGTTAATAANsiIPstIAflIIExtracted region from T4 genome540560580600620640660680700
TCAAAAAATTCATTTTCTGCTTTTTCTTTAAAAGAATCAGCAGCGGATTGGACAGCTGTCCAATCTTTTGAATACCAAGCAACTTGATGCTCGTTTAATTGAATATCATCTTTAAATAAGCTCATATCACTTCCACTGCATTTCGCATGCTAATTGAATGAAAAGATAAGCTAAAAGTTTTTTAAGTAAAAGACGAAAAAGAAATTTTCTTAGTCGTCGCCTAACCTGTCGACAGGTTAGAAAACTTATGGTTCGTTGAACTACGAGCAAATTAACTTATAGTAGAAATTTATTCGAGTATAGTGAAGGTGACGTAAAGCGTACGATTAACTTACTTTTCTATTCGATTTNspISphIPshAIExtracted region from T4 genomehomo1_f1720740760780800820840860
TGCAATTCAGTATTAGCTGCAATACCATGATACTGATTATTTTCGCCGACAATTTCGTACATACGAATAATACTTTGTGGAGTTACACGTGAATAGATTTCTTCGGCAAGTTTACCCACGAACCACGAATAATCAGCCGCATATTTTGGTGCTAAAGCTCTGAGTTGTTTAACATACGTTAAGTCATAATCGACGTTATGGTACTATGACTAATAAAAGCGGCTGTTAAAGCATGTATGCTTATTATGAAACACCTCAATGTGCACTTATCTAAAGAAGCCGTTCAAATGGGTGCTTGGTGCTTATTAGTCGGCGTATAAAACCACGATTTCGAGACTCAACAAATTGTAPmlIBsaAIExtracted region from T4 genomespacer 18809009209409609801,0001,0201,040
CTTTATTTTTGAGAGACTCAAGAACATCATCAATAGCACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTACGAAAATCAGGAAAATTCTTTTTAACCAAAGCTGCTACAACTTTCATATCAGCGAAATAAAAACTCTCTGAGTTCTTGTAGTAGTTATCGTGGTGCTAGCAATCATTGGTCACTATTTTATGGTCGTAGGTTTTGTGGAAATCTGCTTATTGATAGCTCGAGCGGTTATCAAAATGCTTTTAGTCCTTTTAAGAAAAATTGGTTTCGACGATGTTGAAAGTATAGTCGPvuISacIBanIIEco53kIExtracted region from T4 genomespacer 2Spacer 31,0601,0801,1001,1201,1401,1601,1801,2001,220
TATAGCAATTCCTTCATGCTTGCAGATTTCAGTCAATCGACGAATCATCTGCTTCATCATTTCAATTTTATCTTCATCAGTTGGTTGACCGAATGTAATAACTCGGCAGCGTGACTGAAGCGGTTTAATAATACCATCAATATTATTAGCAGTAATAATAATACTACAGTTTGAAATATCGTTAAGGAAGTACGAACGTCTAAAGTCAGTTAGCTGCTTAGTAGACGAAGTAGTAAAGTTAAAATAGAAGTAGTCAACCAACTGGCTTACATTATTGAGCCGTCGCACTGACTTCGCCAAATTATTATGGTAGTTATAATAATCGTCATTATTATTATGATGTCAAACTTHincIIExtracted region from T4 genome21nt Sp...quencespa... 41,2401,2601,2801,3001,3201,3401,3601,3801,400
CTATAAGCTTCCATAAAGGAACGAAGATGTCGCTGAGACTCTGCTAACCCTGAACGGTCAAATTCATCAATAACGATTACTTTTTGACGACCATCAAATGAAGCGGCGCTGGCAAAATTAGTCAAAGGACCACGAACGAAATCAATTTTACAATCTGACCCATTCACAAACATCAGATATTCGAAGGTATTTCCTTGCTTCTACAGCGACTCTGAGACGATTGGGACTTGCCAGTTTAAGTAGTTATTGCTAATGAAAAACTGCTGGTAGTTTACTTCGCCGCGACCGTTTTAATCAGTTTCCTGGTGCTTGCTTTAGTTAAAATGTTAGACTGGGTAAGTGTTTGTAGTHindIIIExtracted region from T4 genome1,4201,4401,4601,4801,5001,5201,5401,560
TATCAGCATTTACATCATGACATAATGCTTTTGCTACAGTTGTTTTACCTGTTCCTGGAGAAGGAGAATGAAGAATAATATGTGGAATCTTACCTTTACTTGTAATAGATTTAAAGGTTTCTTTATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGATAGTCGTAAATGTAGTACTGTATTACGAAAACGATGTCAACAAAATGGACAAGGACCTCTTCCTCTTACTTCTTATTATACACCTTAGAATGGAAATGAACATTATCTAAATTTCCAAAGAAATAGTTTTCGCCCTTCTTATGTAAGTAGCTATCATCTACCTGCTATAAAGACClaIPfoIBspDIBspHIEcoNIExtracted region from T4 genome1,5801,6001,6201,6401,6601,6801,7001,7201,740
TTCAAGAATGTGTTCTTTTTCATTTACAGTAATCATAATTTCCTCATTCAAGTTTTAGTGTAAATTATAAAGGCCGAAGCCCTCTATTAAAAATCGTGGGTAGAATCAGCTTCAAGAGCTACCACATAATTCGCGTGTTCACCTTCAAATTTAGCAGCACCTTGTTTACCTTTTGAAGTTCTTACACAAGAAAAAGTAAATGTCATTAGTATTAAAGGAGTAAGTTCAAAATCACATTTAATATTTCCGGCTTCGGGAGATAATTTTTAGCACCCATCTTAGTCGAAGTTCTCGATGGTGTATTAAGCGCACAAGTGGAAGTTTAAATCGTCGTGGAACAAATGGAAAACExtracted region from T4 genomehomo1_r1homo2_f1spacer 13termina...review1,7601,7801,8001,8201,8401,8601,8801,9001,920
CCCAAAGCAGAAGTTTATAATTTCCTGGTTGCATTTTCATATTTGCCATATTGATAATGAAATTAAATGTATTTTCACCATCATAATCACCAAGAGTCAAAGAATATTTAACACGGGTCAGAGCAGAATCTTCTACTTTATTAAAACCGTTAATTACGATTTTACCTTCTTTTACGGGTTTCGTCTTCAAATATTAAAGGACCAACGTAAAAGTATAAACGGTATAACTATTACTTTAATTTACATAAAAGTGGTAGTATTAGTGGTTCTCAGTTTCTTATAAATTGTGCCCAGTCTCGTCTTAGAAGATGAAATAATTTTGGCAATTAATGCTAAAATGGAAGAAAATGExtracted region from T4 genome1,9401,9601,9802,0002,0202,0402,0602,0802,100
CGTGATAGCAATTGTATCAATTTGCAGACCACGAGATACACGCAACAGCTGTTGAAGGTCTTCAGCTTTAATTTCAGTAACAGCAGATGCTACCGGGAATGGAATTGGTTTATTAGGAGCAACTACTGTACTCGGATCGGCTGCTGGCCAAAAAATTGTTGAGCGGGCATCAGCAGCACTATCGTTAACATAGTTAAACGTCTGGTGCTCTATGTGCGTTGTCGACAACTTCCAGAAGTCGAAATTAAAGTCATTGTCGTCTACGATGGCCCTTACCTTAACCAAATAATCCTCGTTGATGACATGAGCCTAGCCGACGACCGGTTTTTTAACAACTCGCCCGTAGTCGTMfeIMscITatIBssSIBssSαIExtracted region from T4 genome2,1202,1402,1602,1802,2002,2202,2402,260
ATTTTAATATTTCCATCTTCTGACTGGGAAATTTCTGCATCATCATTAACTAAAGACAGAATACCGAGAAAACCGTTCAAATCGTAAATTGCTACATCAAAATCAATAACGTCAGAAATATTTGCTTCCGCATAAGTTGTACCATTAACTGCGCGAGTCATAATAAATTGACCGGTAAAATTATAAAGGTAGAAGACTGACCCTTTAAAGACGTAGTAGTAATTGATTTCTGTCTTATGGCTCTTTTGGCAAGTTTAGCATTTAACGATGTAGTTTTAGTTATTGCAGTCTTTATAAACGAAGGCGTATTCAACATGGTAATTGACGCGCTCAGTATTATTTAACTGGCCExtracted region from T4 genome2,2802,3002,3202,3402,3602,3802,4002,4202,440
ATTTAAGCATAATACCAGAGTTAATAGTAGCGAAATTTTTAAGCAGAGCAGTAGTATCTTTAGACAGTTTCATGTAATTTCCTTCAATTCAAATGAGATTTAATTTTATAACTAATTTAATAAAGCAATTAACGATTAAAATCAGCCGCAATTGTTTCCGCAACAATTTGAGCAGTAAATTCGTATTATGGTCTCAATTATCATCGCTTTAAAAATTCGTCTCGTCATCATAGAAATCTGTCAAAGTACATTAAAGGAAGTTAAGTTTACTCTAAATTAAAATATTGATTAAATTATTTCGTTAATTGCTAATTTTAGTCGGCGTTAACAAAGGCGTTGTTAAACTCGTCMfeIBsaBIExtracted region from T4 genome2,4602,4802,5002,5202,5402,5602,5802,6002,620
CAACAATTAGACGTTCATCTGCCTCGAGTCTGGTAAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCGTCTACTAGCGCAGCTTAATTAACCTAGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTGTTGTTAATCTGCAAGTAGACGGAGCTCAGACCATTTCTTTGGCGACGACGCTTTAAACTTGCGGTCGTGTACCTGAGCAGATGATCGCGTCGAATTAATTGGATCCGACGACGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAccIAvaIPacITliIXhoIAvrIIBsoBIPflMIPspXIPaeR7IEcoO109IExtracte... genomeTrans_clamp_R 2,6402,6602,6802,7002,7202,7402,7602,7802,800
TTGCTGAAACCTCAGGCATTTGAGAAGCACACGGTCACACTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGACCGGGTCATCGTGGCCGGATCTTGCGGCCCCTCGGCTTGAACGAATTGTTAGACATTAACGACTTTGGAGTCCGTAAACTCTTCGTGTGCCAGTGTGACGAAGGCCATCAGTTATTTGGCCATTTGGTCGTTATCTGTATTCGCCGATAAATTGCTGGGACGGGACTTGGCTGCTGGCCCAGTAGCACCGGCCTAGAACGCCGGGGAGCCGAACTTGCTTAACAATCTGTAAAgeIDrdIPflFIBsu36ITth111I2,8202,8402,8602,8802,9002,9202,9402,960
ATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCTAAACGGCTGATGGAACCACTAGAGCGGAAAGTGCATCACCTGTTTAAGAAGGTTGACTAGACGCGCGCTCCGGTTCGCTAGAAGAAGAACAGGTTCTATTCGGACAGATCGAAGTTCATACTGCCCGACTATGACCCGGCCGTCCGCGAGGTAACGGGTCAGCCGTCGCTGTAGBglINaeIBsaAIBssHIIDraIIINgoMIVspect(variant)2,9803,0003,0203,0403,0603,0803,1003,1203,140
CTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGAGAAGCCGCGCTAAAACGGCCAATGACGCGACATGGTTTACGCCCTGTTGCATTCGTGATGTAAAGCGAGTAGCGGTCGGGTCAGCCCGCCGCTCAAGGTATCGCAATTCCAAAGTAAATCGCGGAGTTTATCTAGGACAAGTCCTTGGCCTAGTTTCTCAAGGAGGCGGCGACCTBsmFIspect(variant)3,1603,1803,2003,2203,2403,2603,2803,3003,320
CCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGGGATGGTTCCGTTGCGATACAAGAGAACGAAAACAGTCGTTCTATCGGTCTAGTTACAGCTAGCACCGACCGAGCTTCTATGGACGTTCTTACAGTAACGCGACGGTAAGAGGTTTAACGTCAAGCGCGAATCGACCTATTGCGGTGCCTTACTACAGCAGCACGTGTTGTTACCAjuIPvuIApaLIBfuAIBspMIspect(variant)3,3403,3603,3803,4003,4203,4403,4603,4803,500
TGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACACTGAAGATGTCGCGCCTCTTAGAGCGAGAGAGGTCCCCTTCGGCTTCAAAGGTTTTCCAGCAACTAGTTTCGAGCGGCGCAACAAAGTAGTTCGGAATGCCAGTGGCATTGGTCGTTTAGTTATAGTGACACACCGAAGTCCGGCGGTAGGTGACGCCTCGGCATGTTTACATGBclIBstEIIDraIIIspect(variant)3,5203,5403,5603,5803,6003,6203,6403,660
GGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATCCGGTCGTTGCAGCCAAGCTCTACCGCGAGCTACTGCGGTTGATGGAGACTATCAACTCAGCTATGAAGCCGCTAGTGGCGAAGGGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTABspHIspect(variant)AmpR_promoter3,6803,7003,7203,7403,7603,7803,8003,8203,840
AAACAAATAGCTAGCTCACTCGGTCGCTACGCTCCGGGCGTGAGACTGCGGCGGGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAGCAGGGCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGTTTGTTTATCGATCGAGTGAGCCAGCGATGCGAGGCCCGCACTCTGACGCCGCCCGCGACGCCTGTGTATGTTTCAATGGGTGTCTAAGGCACCTATTCGTCCCCTGATTGTACACTCCGTTTTGTCGTCCCGGCGCGGCCACCGCAAAAAGGTATCCGAGGCGGGAGGACGGTCBmtINheINspIPciIBsmFISgrAI3,8603,8803,9003,9203,9403,9603,9804,0004,020
AGTTCACATAAACAGACGCTTTTCCGGTGCATCTGTGGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCCGGCGGGTCGCTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGTCAAGTGTATTTGTCTGCGAAAAGGCCACGTAGACACCCTCGGCACTCCGAGTTGGTACTTAGACTGTCATGCCCGCTTTGGGCTGTCCTGAATTTCTAGGGGTGGCAAAGGCCGCCCAGCGAGGGAGAACGCGAGAGGACAAGGCTGGGACGGCAAATGGCCTATGGACAAGGC4,0404,0604,0804,1004,1204,1404,1604,1804,200
CCTTTCTCCCTTACGGGAAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCAAGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACCCGGAAAAGCACGGTAAGGAAAGAGGGAATGCCCTTCACACCGCGAAAGAGTATCGAGTGTGTGACCATAGAGCCGAGCCACATCCAGCAAGCGAGGTTCGACCCGACATTCGTTCTTGAGGGGCAAGTCGGGCTGACGACGCGGAATAGGCCATTGACAAGTGAACTCAGGTTGGGCCTTTTCGTGCCATTBaeI4,2204,2404,2604,2804,3004,3204,3404,360
AACGCCACTGGCAGCAGCCATTGGTAACTGGGAGTTCGCAGAGGATTTGTTTAGCTAAACACGCGGTTGCTCTTGAAGTGTGCGCCAAAGTCCGGCTACACTGGAAGGACAGATTTGGTTGCTGTGCTCTGCGAAAGCCAGTTACCACGGTTAAGCAGTTCCCCAACTGACTTAATTGCGGTGACCGTCGTCGGTAACCATTGACCCTCAAGCGTCTCCTAAACAAATCGATTTGTGCGCCAACGAGAACTTCACACGCGGTTTCAGGCCGATGTGACCTTCCTGTCTAAACCAACGACACGAGACGCTTTCGGTCAATGGTGCCAATTCGTCAAGGGGTTGACTGAATT4,3804,4004,4204,4404,4604,4804,5004,5204,540
CCTTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACTGAACCGCTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCCCATACGATATAAGTGGAAGCTAGTTTGGTGGAGGGGTCCACCAAAAAAGCAAATGTCCCGTTTTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGACTTGGCGAGATCTAAAGTCACGTTAAATAGAGAAGTTTACATCGTGGACTTCAGTCGGGGTATGCTATATTCAXbaI4,5604,5804,6004,6204,6404,6604,6804,7004,720
TGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAAACATTAAGAGTACAATCAGTACGGGGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGGGCCACGGATTACTCACTCGATTGAATGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATT4,7404,7604,7804,8004,8204,8404,8604,8804,900
TGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGTCGTCCGCTTTTAGGACAAACTACCACKasINarISfoIBsaXIBsmBIEsp3IPluTI4,9204,9404,9604,9805,0005,0205,0405,060
GTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGCAATTGCCGCCCTATATTGTACTCGACAGAAGCCATAGCAGCATAGGGTGATGGCTCTACAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACGGGAGTAAGTCGTAAACGTACCHpaIBssHIIHincII5,0805,1005,1205,1405,1605,1805,2005,2205,240
TTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTACGCTGGTCTACGAGGTGApaIBanIIBstEIIPspOMI5,2605,2805,3005,3205,3405,3605,3805,4005,420
GCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGCGCAACGCGCTCTTCTAACBclIBsgIMluI5,4405,4605,4805,5005,5205,5405,5605,5805,600
TGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGACAAACGGGCGGTCAACAABsgIApaLI5,6205,6405,6605,6805,7005,7205,7405,760
GTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTCTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGACTTAclIBstAPI5,7805,8005,8205,8405,8605,8805,9005,9205,940
TTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAATTAATACGACTCACTATAAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTTAATTATGCTGAGTGATATPfoIEcoNIPflMI5,9605,9806,0006,0206,0406,060
  • Plasmid
Editing disabled on read-only sequence.
6075 bp45005000550050010001500HindIIIPAM-cas12a

Welcome to Benchling!

Sign in to view data.

New digest

Saved digests

Enzyme AatII
Enzyme lists
Cut sites visible on maps
Find enzyme
Name Cuts
Selected Color
Show enzymes that cut

Primers

History

Lineage

 Show Full History
Change User Timestamp

Sequence Alignments

Saved Alignments

Sequence Info

Tags

Name
Parent Version
Folder
Topology
Start Index
Locked
Created
--
Length
--
Export Data
Split Workspace
Bases
6075