Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pRCGnagGHcomp
  • Sequence Map
    Editing disabled on read-only sequence.
    gggcgaattgggcccgacgtcgcatgctcccggccgccatggccgcgggatttgaccgagcagatgccggtggtgacggtcgcgcccaacgacgcgctgctggagaagctcaagtccaacatccaggaagtgcgcgcgcgcggcggcaagctctacgtgttcgccgatgccgacacgccccgcttaacccgggctgcagcgtacgagggccggcggtaccggcgccctaaactggctcgtctacggccaccactgccagcgcgggttgctgcgcgacgacctcttcgagttcaggttgtaggtccttcacgcgcgcgcgccgccgttcgagatgcacaagcggctacggctgtgcgApaIPfoISfiIPspOMIglmS\RSc0178Recombination\Region\120406080100120140160
    atatccagtcgagcgacggcatccaggtgatccgcatgcccgagcactatgggcagctgtcgccgatcctgcacgtggtgccgctgcagctgctggcgtatcacaccgccgtggcgcgcgggacggatgtggataagccgcggaatttggctaagtctgtgacggtggagtgagggggtataggtcagctcgctgccgtaggtccactaggcgtacgggctcgtgatacccgtcgacagcggctaggacgtgcaccacggcgacgtcgacgaccgcatagtgtggcggcaccgcgcgccctgcctacacctattcggcgccttaaaccgattcagacactgccacctcactcccccAleIBstAPIRecombination\Region\1glmS\RSc0178180200220240260280300320340
    cggtagtgctgttgtccttgtaacggcagacaaaaaagtctgtaccaggtccgcagttgaaacgtataggcaccggtctgccccgattaagcggggggccggtgtttttttcgaataggtgttggctcagaggaggcgggcgccgccgctgtttcccagaccttagtcgtagggtgacgccatcacgacaacaggaacattgccgtctgttttttcagacatggtccaggcgtcaactttgcatatccgtggccagacggggctaattcgccccccggccacaaaaaaagcttatccacaaccgagtctcctccgcccgcggcggcgacaaagggtctggaatcagcatcccactgAgeIBstBIRecombination\Region\1360380400420440460480500520
    cagcggacccggtcgtagcaggccgacggcaattcgatgctcaacgttccgaatttatccagcagcttcttggcgagcttgggtgacgcgacctgttccgcgtataaggtgactaggtgtctcacctcatatcggcgcactttgcgatcgcttttccgatcgaccgactgagacgattgtcgcctgggccagcatcgtccggctgccgttaagctacgagttgcaaggcttaaataggtcgtcgaagaaccgctcgaacccactgcgctggacaaggcgcatattccactgatccacagagtggagtatagccgcgtgaaacgctagcgaaaaggctagctggctgactctgctaaAsiSIRecombination\Region\1540560580600620640660680700
    caacgttttcttcgggaaagcacgctgtttccgctatttgagatgttcactggggcgcaattctctgcccgggaagaacggggccgcatcgatgcggtacttcaaagcctcccaaagcgcatggttggcgcattcggggggccgactcttgtgtccgcttgatgccacgtctcggatcgttgcaaaagaagccctttcgtgcgacaaaggcgataaactctacaagtgaccccgcgttaagagacgggcccttcttgccccggcgtagctacgccatgaagtttcggagggtttcgcgtaccaaccgcgtaagccccccggctgagaacacaggcgaactacggtgcagagcctagClaIBspDIRecombination\Region\1720740760780800820840860880
    gtgagccccggtcgatagttggagcctcttccactgcccaggccggcggctcgccatcgttctatatcggcaatcatgatgttgcggcgcttcttcataactgatcagctcaatcctgtcgcccactcgagggatcccccgggaacccggccgcgtccggcgcccccgccgccttcgacactcggggccagctatcaacctcggagaaggtgacgggtccggccgccgagcggtagcaagatatagccgttagtactacaacgccgcgaagaagtattgactagtcgagttaggacagcgggtgagctccctagggggcccttgggccggcgcaggccgcgggggcggcggaagctPspXIRecombination\Region\1tfd19009209409609801,0001,0201,0401,060
    cgagatcccgcaaaagcggcctttgactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagccgctctagggcgttttcgccggaaactgagggacgttcggagtcgctggcttatatagccaatacgcacccgctaccaacaacagtaacagccgcgttgatagccatagttcgacaaattctttaagtggagctttcgttcgactatttggctatgttaatttccgaggaaaacctcggBbvCItfd11,0801,1001,1201,1401,1601,1801,2001,2201,240
    tttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaacctcatacagaaaattcatttactaacgtctggaaagacgacaaaactttgaattccccgggaacccggccgaaaaaaaaacctctaaaagttgcacttttttaataataagcgttaaggaaatcaacaaggaaagataagagtgaggcgactttgacaactttcaacaaatcgttttggagtatgtcttttaagtaaatgattgcagacctttctgctgttttgaaacttaaggggcccttgggccggctfd1tfd21,2601,2801,3001,3201,3401,3601,3801,4001,420
    cgtccggcgcccccgccgccttcgacgagatcccgcaaaagcggcctttgactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgataaaccgatgcaggccgcgggggcggcggaagctgctctagggcgttttcgccggaaactgagggacgttcggagtcgctggcttatatagccaatacgcacccgctaccaacaacagtaacagccgcgttgatagccatagttcgacaaattctttaagtggagctttcgttcgactatttggctaBbvCItfd21,4401,4601,4801,5001,5201,5401,5601,5801,600
    acaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaacctcatacagaaaattcatttactaacgtctggaaagacgacaaaactgttaatttccgaggaaaacctcggaaaaaaaaacctctaaaagttgcacttttttaataataagcgttaaggaaatcaacaaggaaagataagagtgaggcgactttgacaactttcaacaaatcgttttggagtatgtcttttaagtaaatgattgcagacctttctgctgttttgtfd21,6201,6401,6601,6801,7001,7201,7401,7601,780
    tttgatatctcatgagctcagccaatcgactggcgagcggcatcgcattcttcgcatcccgccctctggcggatgcaggaagatcaacggatctcggcccagttgacccagggctgtcgccacaatgtcgcgggagcggatcaaccgagcaaaggcatgaccgactggaccttccttcaaactatagagtactcgagtcggttagctgaccgctcgccgtagcgtaagaagcgtagggcgggagaccgcctacgtccttctagttgcctagagccgggtcaactgggtcccgacagcggtgttacagcgccctcgcctagttggctcgtttccgtactggctgacctggaaggaagPasIEcoRVApramycin/Gentamicin\Resistance1,8001,8201,8401,8601,8801,9001,9201,940
    tgaaggctcttctccttgagccacctgtccgccaaggcaaagcgctcacagcagtggtcattctcgagataatcgacgcgtaccaacttgccatcctgaagaatggtgcagtgtctcggcaccccatagggaacctttgccatcaactcggcaagatgcagcgtcgtgttggcatcgtacttccgagaagaggaactcggtggacaggcggttccgtttcgcgagtgtcgtcaccagtaagagctctattagctgcgcatggttgaacggtaggacttcttaccacgtcacagagccgtggggtatcccttggaaacggtagttgagccgttctacgtcgcagcacaaccgtagcaAfeIAleIApramycin/Gentamicin\Resistance1,9601,9802,0002,0202,0402,0602,0802,1002,120
    gtcccacgccgaggagaagtacctgcccatcgagttcatggacacgggcgaccgggcttgcaggcgagtgaggtggcaggggcaatggatcagagatgatctgctctgcctgtggccccgctgccgcaaaggcaaatggatgggcgctgcgctttacatttggcaggcgccagaatgtcagggtgcggctcctcttcatggacgggtagctcaagtacctgtgcccgctggcccgaacgtccgctcactccaccgtccccgttacctagtctctactagacgagacggacaccggggcgacggcgtttccgtttacctacccgcgacgcgaaatgtaaaccgtccgcggtcttacaApramycin/Gentamicin\Resistance2,1402,1602,1802,2002,2202,2402,2602,2802,300
    gtcagagacaactccaaggtccggtgtaacgggcgacgtggcaggatcgaacggctcgtcgtccagacctgaccacgagggcatgacgagcgtccctcccggacccagcgcagcacgcagggcctcgatcagtccaagtggcccatcttcgaggggccggacgctacggaaggagctgcagtctctgttgaggttccaggccacattgcccgctgcaccgtcctagcttgccgagcagcaggtctggactggtgctcccgtactgctcgcagggagggcctgggtcgcgtcgtgcgtcccggagctagtcaggttcaccgggtagaagctccccggcctgcgatgccttcctcgacBplIBplIPfoIApramycin/Gentamicin\Resistance2,3202,3402,3602,3802,4002,4202,4402,4602,480
    tggaccagcagcacaccgccgggggtaaccccaaggttgagaagctgaccgatgagctcggcttttcgccattcgtattgcacgacattgcactccaccgctgatgacatcagtcgatcatagcacgatcaacggcactgttgcaaatagtcggtggtgataaacttatcatccccttacctggtcgtcgtgtggcggcccccattggggttccaactcttcgactggctactcgagccgaaaagcggtaagcataacgtgctgtaacgtgaggtggcgactactgtagtcagctagtatcgtgctagttgccgtgacaacgtttatcagccaccactatttgaatagtaggggaaApramycin/Gentamicin\Resistance2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ttgctgatggagctgcacatgaacccctagggttaacggtaccatctcatcgcgcaacgtatcggcgtggttgcgcaccgtactgaccgtgacgccgggcgcgtccctggccagccggcgcatcagcgtcggcaggaaatagatctcgccgatgtccgtcatcgcgatcgtgaaggcgaacgactacctcgacgtgtacttggggatcccaattgccatggtagagtagcgcgttgcatagccgcaccaacgcgtggcatgactggcactgcggcccgcgcagggaccggtcggccgcgtagtcgcagccgtcctttatctagagcggctacaggcagtagcgctagcacttccgcHpaIKpnIAvrIIBglIIAcc65I2,6802,7002,7202,7402,7602,7802,8002,8202,840
    cgcgtgctggtggcgggctcgaacctggaggtggcgttgagcgagttgtagatcgccccgagcgcgtaggcgatcggctcggcgagcgtctcggcaaacggcgtcgggatcatccctttcgatgtgcggacgaacagttcatcccccagcagcttgcgcagccggttcagcgcattgcgcgcacgaccaccgcccgagcttggacctccaccgcaactcgctcaacatctagcggggctcgcgcatccgctagccgagccgctcgcagagccgtttgccgcagccctagtagggaaagctacacgcctgcttgtcaagtagggggtcgtcgaacgcgtcggccaagtcgcgtaacg2,8602,8802,9002,9202,9402,9602,9803,0003,020
    tgatcgccggctgcgtgatgccgagggtctcggcgacggccgaaacgcgccgctgcctgagcaattcgttgaagacgaccagcaggttgaggtcgatgtcttgcagttccatggcgtgtctcctcgccgtctcgcattatcactgcgagtgatatgacacatcaggttgcttctatttactagcggccgacgcactacggctcccagagccgctgccggctttgcgcggcgacggactcgttaagcaacttctgctggtcgtccaactccagctacagaacgtcaaggtaccgcacagaggagcggcagagcgtaatagtgacgctcactatactgtgtagtccaacgaagataaa3,0403,0603,0803,1003,1203,1403,1603,1803,200
    atggatggcgtcaagcgcgtcatgatgcgtccacacctacagacagaccagcccggagacactggccatggacgtcaccctcaccccgctgcagcgcgtcgtcagcgcgcgcgccggcgataacctgctcgaagtgctgcgcgcgcatcaggttcccatctcgtacagctgcctgtcgtacctaccgcagttcgcgcagtactacgcaggtgtggatgtctgtctggtcgggcctctgtgaccggtacctgcagtgggagtggggcgacgtcgcgcagcagtcgcgcgcgcggccgctattggacgagcttcacgacgcgcgcgtagtccaagggtagagcatgtcgacggacagcMreISgrAInagAa3,2203,2403,2603,2803,3003,3203,3403,3603,380
    ggacgctgcggaacgtgccgctgccgcgtgctctcgggcaacgtcgtcgccaccggcggcaccgagatcaacgcgccctccgcgccgggccagaccgtgctggcctgccagacgacgctggtcgaagactgcgcgatcgaactgcccgaggtggacgagatcgtcgtgcacccggccacctgcgacgccttgcacggcgacggcgcacgagagcccgttgcagcagcggtggccgccgtggctctagttgcgcgggaggcgcggcccggtctggcacgaccggacggtctgctgcgaccagcttctgacgcgctagcttgacgggctccacctgctctagcagcacgtgggccggtSgrAInagAa3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ggatcatcaaatcgaaggtcgtcgcgatcgaggacatgacgcacgacatcaagcggatccgcctggagctggccaagccgctcgcgttctcgccgggccagtacgcgaccctgcagttcacgccgcggcatgtgcggccctactccatggcgttcgccgatgcgggacaggcgctggacctagtagtttagcttccagcagcgctagctcctgtactgcgtgctgtagttcgcctaggcggacctcgaccggttcggcgagcgcaagagcggcccggtcatgcgctgggacgtcaagtgcggcgccgtacacgccgggatgaggtaccgcaagcggctacgccctgtccgcgacctnagAa3,5803,6003,6203,6403,6603,6803,7003,720
    gttccatgtgcggctggtgccgggcggacgcgtgacctcctacgtggcggccgaactcaaggtcggggacgacgtgcgcgtcagcggcccgctgggcacggcctacctgcgccgtaaaaacaccgatccggtcatctgcgtggccggcggcacggggcttgcgcccatcctctccatccaaggtacacgccgaccacggcccgcctgcgcactggaggatgcaccgccggcttgagttccagcccctgctgcacgcgcagtcgccgggcgacccgtgccggatggacgcggcatttttgtggctaggccagtagacgcaccggccgccgtgccccgaacgcgggtaggagaggtagnagAa3,7403,7603,7803,8003,8203,8403,8603,8803,900
    ctgcggggcatggccgaggccggcatggccaatccggtccacgtctacttcggcgtgcgctcgccggccgatgtgtacggaacgcactggctggacgccctgcgcgaacgcctgccgaacttgcacacgcatgtcgtcgtcgccacctccagcgcggatgcgcgctaccgctcgggcggacgccccgtaccggctccggccgtaccggttaggccaggtgcagatgaagccgcacgcgagcggccggctacacatgccttgcgtgaccgacctgcgggacgcgcttgcggacggcttgaacgtgtgcgtacagcagcagcggtggaggtcgcgcctacgcgcgatggcgagcccgcSfiInagAa3,9203,9403,9603,9804,0004,0204,0404,0604,080
    tcgtcaccgacgccgtcgcgagcgactggggcgacctgcggggatggcgcgcctacctggccggggcaccggtcatggtggatgcggccagcctgttgctacgccagcgcggcgtgccggccgaacacgtctacgccgatgcgttctacgcggtgggcgtctgagcctccccccaccgagcagtggctgcggcagcgctcgctgaccccgctggacgcccctaccgcgcggatggaccggccccgtggccagtaccacctacgccggtcggacaacgatgcggtcgcgccgcacggccggcttgtgcagatgcggctacgcaagatgcgccacccgcagactcggaggggggtggcAgeIAscInagAa4,1004,1204,1404,1604,1804,2004,2204,2404,260
    gcctccacaaaaacacaggagacaaagcatgaaggaatccccgctcagcttctcgaagccgctgtggaaggacagcggatcgagccgcatcccgttccgggcctacaccgacgaggcggtctaccagcgcgagctggagcggctgttctatgccggccactggtgctacgtcggcctccggaggtgtttttgtgtcctctgtttcgtacttccttaggggcgagtcgaagagcttcggcgacaccttcctgtcgcctagctcggcgtagggcaaggcccggatgtggctgctccgccagatggtcgcgctcgacctcgccgacaagatacggccggtgaccacgatgcagccggagBarInagG4,2804,3004,3204,3404,3604,3804,4004,4204,440
    gaagccgagatacccaacccgggcgatttcaagcggacggcgatcggcgagcgctcggtcatcgtcacgcgcacgccggatcacggcatcgcggtggtcgagaatgtctgcgcgcaccgcggcgtgcgcttctgcagagagaagtcgggcaaccgcaaggacttcacctgcccctatccttcggctctatgggttgggcccgctaaagttcgcctgccgctagccgctcgcgagccagtagcagtgcgcgtgcggcctagtgccgtagcgccaccagctcttacagacgcgcgtggcgccgcacgcgaagacgtctctcttcagcccgttggcgttcctgaagtggacggggatagAfeIPaqCInagG4,4604,4804,5004,5204,5404,5604,5804,6004,620
    accagtggaactacgacctgaagggcaacctgatcggcgtgccgttccgccggggcgtcaaggccgacggcaaggtcaacggcggcatgccgagcgacttcgacccgaagcagcacggcctcacccaactgaacgtggccgtgcgcaacggcgtcatcttcgcgtcgttcgaccacgatggtcaccttgatgctggacttcccgttggactagccgcacggcaaggcggccccgcagttccggctgccgttccagttgccgccgtacggctcgctgaagctgggcttcgtcgtgccggagtgggttgacttgcaccggcacgcgttgccgcagtagaagcgcagcaagctggtgctnagG4,6404,6604,6804,7004,7204,7404,7604,7804,800
    cctgccgtcgctcgaagcgtttctcggccccaccatcctgggctacttcgatcgggtcttcgacgggcgcaagctgaagattctcggctacaaccgccagcgcattccgggcaactggaagctcatgcaggagaacatcaaggacccctaccaccccggcctgctccatacgtggttcggacggcagcgagcttcgcaaagagccggggtggtaggacccgatgaagctagcccagaagctgcccgcgttcgacttctaagagccgatgttggcggtcgcgtaaggcccgttgaccttcgagtacgtcctcttgtagttcctggggatggtggggccggacgaggtatgcaccaagXmnIPpuMInagG4,8204,8404,8604,8804,9004,9204,9404,9604,980
    gtcacgttcgggctctggcgcgccgacaacaagtcggaactgaagatggatgcgctgcatcgccacgcggcgatgatctccacgcgcggatcgggcggcaagggcgacgtgacctcgggcgtcagcagcttcaaggagaagatggcgctgcacgacccgcgctttctcgacgtcgtgccagtgcaagcccgagaccgcgcggctgttgttcagccttgacttctacctacgcgacgtagcggtgcgccgctactagaggtgcgcgcctagcccgccgttcccgctgcactggagcccgcagtcgtcgaagttcctcttctaccgcgacgtgctgggcgcgaaagagctgcagcacgAscInagG5,0005,0205,0405,0605,0805,1005,1205,1405,160
    atgagccgtggtggggcacgccgaccgccgtgatgatgacgatctttccgagcgtgatcatccagcagcaggtcaactcggtctcgacgcggcacatccagccgaacggccacggatcgttcgactttgtctggacccacttcggcttcgaggacgacaccgaagacatgacgcagcgtactcggcaccaccccgtgcggctggcggcactactactgctagaaaggctcgcactagtaggtcgtcgtccagttgagccagagctgcgccgtgtaggtcggcttgccggtgcctagcaagctgaaacagacctgggtgaagccgaagctcctgctgtggcttctgtactgcgtcgcBsaBInagG5,1805,2005,2205,2405,2605,2805,3005,3205,340
    ccgcctgcgccaggccaacctgttcggcccggccggcttcgtgtcggcggacgacggcgaagtcatcgagttctcgcaggaaggcttcgagcagaagcccggccaccagaccctcgccgagctgggcggacgcgagatcggcgagaccgaccacatggtcaccgagacgctgatccgcggcggacgcggtccggttggacaagccgggccggccgaagcacagccgcctgctgccgcttcagtagctcaagagcgtccttccgaagctcgtcttcgggccggtggtctgggagcggctcgacccgcctgcgctctagccgctctggctggtgtaccagtggctctgcgactaggcgnagG5,3605,3805,4005,4205,4405,4605,4805,500
    ggcatgtacgaatactggcgcacggtgatggagctctgacatgcttgatttcgcgacctaccagcagttgatcgatttctatacccgctatgccgccgccgtggacaacggcgactggcacgcctggcccgagttcttcctcgaccaatgcatctacaaggtccagccgcgcgaaaacccgtacatgcttatgaccgcgtgccactacctcgagactgtacgaactaaagcgctggatggtcgtcaactagctaaagatatgggcgatacggcggcggcacctgttgccgctgaccgtgcggaccgggctcaagaaggagctggttacgtagatgttccaggtcggcgcgcttttgClaIBspDInagHnagG5,5205,5405,5605,5805,6005,6205,6405,6605,680
    cacgagcgcggctacccgctggccaccctgtcgttcgaaagcaaggggatgctcaaggaccgggtgtacggaatgaccgagacgatcttccacgacccctactaccagcgccacgtgatcagcctgccccaggtgacgcgcgtcgagggcgaccgcatcgaggcacaggccaattacggtgctcgcgccgatgggcgaccggtgggacagcaagctttcgttcccctacgagttcctggcccacatgccttactggctctgctagaaggtgctggggatgatggtcgcggtgcactagtcggacggggtccactgcgcgcagctcccgctggcgtagctccgtgtccggttaatgcBstBInagH5,7005,7205,7405,7605,7805,8005,8205,8405,860
    ccgtgttccgcaccaagccggacgagctgtcgacggtcttcaacgtcggccgctacatcgacgcgatcgtccgcacggacgacgggctgaagcttgcgtcgcgcctctgcgtgttcgacagcgaaatgatccccaactcgatcatctatccgatctgagacctgccatgacgacaaccggcacaaggcgtggttcggcctgctcgacagctgccagaagttgcagccggcgatgtagctgcgctagcaggcgtgcctgctgcccgacttcgaacgcagcgcggagacgcacaagctgtcgctttactaggggttgagctagtagataggctagactctggacggtactgctgttggSalIHindIIInagH5,8805,9005,9205,9405,9605,9806,0006,0206,040
    tggttcaagctcataagggcgaattctgcagatatccatcacactggcggccgctcgagcatgcatcgagctcggatccactagtaacggccgccagtgtgctggaattcgccctttctagaagatcttagactagggtcgaggatccggctgctaacaaagcccgaaaggaagctgaaccaagttcgagtattcccgcttaagacgtctataggtagtgtgaccgccggcgagctcgtacgtagctcgagcctaggtgatcattgccggcggtcacacgaccttaagcgggaaagatcttctagaatctgatcccagctcctaggccgacgattgtttcgggctttccttcgactNotISpeIXbaIBglIIBsaBIEcoRVPspXIattR1attR26,0606,0806,1006,1206,1406,1606,1806,2006,220
    gttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaattcgactagtggtcgatgtcttggggccagcgattgtatcgggggatactccccatcagctattcagaaccctcaagacaactctggacatcagcggcaaccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgacttaagctgatcaccagctacagaaccccggtcgctaacatagccccctatgaggggtagtcgataagtcttgggagttctgttgagacctgtagtcgccSpeIRecombination\Region\2attR1attR2t7\terminatorlac_promoter6,2406,2606,2806,3006,3206,3406,3606,3806,400
    ctgccacttgtccaaatcgcatcatggcaacgagacattggtggttgctatggagagagggcttgcacgccaaaagccagcgtcgtccctcactcctcaacctcgctgcgaaccatcagcacaggtttgttcgtcttgcgcactacgccttcggccacactgccaagcaccaggtgttgacggtgaacaggtttagcgtagtaccgttgctctgtaaccaccaacgatacctctctcccgaacgtgcggttttcggtcgcagcagggagtgaggagttggagcgacgcttggtagtcgtgtccaaacaagcagaacgcgtgatgcggaagccggtgtgacggttcgtggtccacaaRecombination\Region\2CAT/CamRRSc01796,4206,4406,4606,4806,5006,5206,5406,5606,580
    tgagcccgcgccggccgtgagtaccaagcacaatcaggtcagtgccggtctcgttggcctcggccacaatcgttgcggaaatctgccccggcacaatggatttctctagcaacttggtcgaatgcttgacgccagcggcctccaatttggtcgcaacctcggccagcgtgtctcggccactcgggcgcggccggcactcatggttcgtgttagtccagtcacggccagagcaaccggagccggtgttagcaacgcctttagacggggccgtgttacctaaagagatcgttgaaccagcttacgaactgcggtcgccggaggttaaaccagcgttggagccggtcgcacagagccggCspCIRecombination\Region\2CAT/CamRRSc01796,6006,6206,6406,6606,6806,7006,7206,7406,760
    gaactcgacaacctgtttggcgtaggcaacggagtcagccccgaccatgtcgaaggtcacgtcgctgctatcggcaacaaagagggcttcgatttcggcgcccgtcgctttgctgaccacaatcgcctggtgcagggcgagataggacgcgtggctgccgtcgatggcgagcaggatgcttgagctgttggacaaaccgcatccgttgcctcagtcggggctggtacagcttccagtgcagcgacgatagccgttgtttctcccgaagctaaagccgcgggcagcgaaacgactggtgttagcggaccacgtcccgctctatcctgcgcaccgacggcagctaccgctcgtcctacRecombination\Region\2CAT/CamRRSc01796,7806,8006,8206,8406,8606,8806,9006,9206,940
    cgttggtacatgacggtctccaaaggcgttgtgcgggacagtgccgcatgtcaccagcgtaggcggcaggatgctgggctcattgacccatatcaacgctcttccggggcgattcccaataccgccgcgcaggcaatgcgcccggcacgcaggttgtccagagcggtgttggtttcctgcaaccatgtactgccagaggtttccgcaacacgccctgtcacggcgtacagtggtcgcatccgccgtcctacgacccgagtaactgggtatagttgcgagaaggccccgctaagggttatggcggcgcgtccgttacgcgggccgtgcgtccaacaggtctcgccacaaccaaaggaBstAPIRecombination\Region\2CAT/CamRpseudogene\(btw\RSc0179-RSc0180\)RS...96,9606,9807,0007,0207,0407,0607,0807,1007,120
    gcagtcgatacggcatgacgtgaacctgcaggggtactgcagccgccacgtgcatgagcgccaggccatccgatcgcgtcaggttagccactgagacgagcttgcgctcctcccacagcagccggtacggaaacgtggggatgtcgctcatggggctgccaccgcaaaccacggaagccgtcagctatgccgtactgcacttggacgtccccatgacgtcggcggtgcacgtactcgcggtccggtaggctagcgcagtccaatcggtgactctgctcgaacgcgaggagggtgtcgtcggccatgcctttgcacccctacagcgagtaccccgacggtggcgtttggtgccttcgSbfIHindIIIRecombination\Region\2pseudogene\(btw\RSc0179-RSc0180\)7,1407,1607,1807,2007,2207,2407,2607,280
    ttgtcgaccatatgggagagctcccaacgcgttggatgcatagcttgagtattctatagtgtcacctaaatagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcaacagctggtataccctctcgagggttgcgcaacctacgtatcgaactcataagatatcacagtggatttatcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacgNdeISalIccdB7,3007,3207,3407,3607,3807,4007,4207,4407,460
    ctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacccdB7,4807,5007,5207,5407,5607,5807,6007,6207,640
    cggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgPciIattR2attR1ccdB7,6607,6807,7007,7207,7407,7607,7807,8007,820
    gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttccgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagHAattR1attR27,8407,8607,8807,9007,9207,9407,9607,9808,000
    ggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacaccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgt8,0208,0408,0608,0808,1008,1208,1408,1608,180
    gagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaactcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtcttttt8,2008,2208,2408,2608,2808,3008,3208,3408,360
    aaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgattcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagact8,3808,4008,4208,4408,4608,4808,5008,5208,540
    cagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcagtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgtbeta-lactamase\(Ampr)8,5608,5808,6008,6208,6408,6608,6808,7008,720
    ataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggtatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccbeta-lactamase\(Ampr)8,7408,7608,7808,8008,8208,8408,8608,8808,900
    cttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaggaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattcbeta-lactamase\(Ampr)8,9208,9408,9608,9809,0009,0209,0409,060
    atgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgtacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcScaITatIXmnIbeta-lactamase\(Ampr)9,0809,1009,1209,1409,1609,1809,2009,2209,240
    ctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttSspIbeta-lactamase\(Ampr)9,2609,2809,3009,3209,3409,3609,3809,4009,420
    gcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggaaattgtaagcgttaatattttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactacgccacactttatggcgtgtctacgcattcctcttttatggcgtagtcctttaacattcgcaattataaaaSspI9,4409,4609,4809,5009,5209,5409,5609,5809,600
    gttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaacccaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggPsiI9,6209,6409,6609,6809,7009,7209,7409,7609,780
    gtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctacagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgat9,8009,8209,8409,8609,8809,9009,9209,9409,960
    gggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgacccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcaggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgct9,98010,00010,02010,04010,06010,08010,10010,12010,140
    ttaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactataaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatat10,16010,18010,20010,220
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10226 bp750080008500900095005001000150020002500attR2glmS\RSc0178lloc\clonatge,Recombination\Region\1,PspOMI,ApaI,SfiI,PfoI,BstAPI,AleI,AgeI,BstBIAsiSI,BspDI,ClaIPspXItfd1,BbvCItfd2,BbvCIEcoRVPasI,AfeI,AleIApramycin/Gentamicin\ResistanceBplIPfoI,BplIattR1,PciIMutation\eliminated\EcoRV\and\HindIIIHAbeta-lactamase\(Ampr)TatI,ScaI,XmnISspI,SspIPsiI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    10226