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pdf0112cmv-cluciferase-polya-ef1a-g-luciferase-polya
  • Sequence Map
    Editing disabled on read-only sequence.
    agccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatttcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaaSapIBspQILac-operonpBR32220406080100120140160
    aggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaCACACAAAAAACCAACACACAGATGTAATGAAAATAAAGATATTTTATTTTAtttgcattcatctggtactccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggtGTGTGTTTTTTGGTTGTGTGTCTACATTACTTTTATTTCTATAAAATAAAATaaacgtaagtagaccatgLac-operonpolyA signalHindIII GHCTTTGC...GGGTGCLucTransl...1-333180200220240260280300320340
    ttctagggtgtctccatgctttatgtagcattctttcttgaactcccatgcgtggtcacagaaaccctgttgtcccctcaggcaatactcgtacatgcatcgttcgacacggtcaggcttgtggcacacgttacatttctgatcaagatcactttgaccggcgaagagactattgaagatcccacagaggtacgaaatacatcgtaagaaagaacttgagggtacgcaccagtgtctttgggacaacaggggagtccgttatgagcatgtacgtagcaagctgtgccagtccgaacaccgtgtgcaatgtaaagactagttctagtgaaactggccgcttctctgataacBclINsiIPflFIBsu36ITth111ICLucTranslation 1991-333CT...G360380400420440460480500520
    cagagtcgttcagcttcaggtttctgttcttcggtgcatcccggtgggttgggggtcagatcacaggctccttcagcatcaaaagaatcatcactgaaatcctggttgtagttaccgcaaataccgcaagtcttaccatcgaatggatctctaatatgtacgacgagcagttgctgtctcagcaagtcgaagtccaaagacaagaagccacgtagggccacccaacccccagtctagtgtccgaggaagtcgtagttttcttagtagtgactttaggaccaacatcaatggcgtttatggcgttcagaatggtagcttacctagagattatacatgctgctcgtcaacgaCLucTranslation 1991-333540560580600620640660680700
    tgaagttgaacttgaccaccagagcttcaggtaggatggctgtagtcagtatgtcaccatcttgccagtagatggaagtgttctgagagctgtacgggacggacacggcttctcctccaaccagaatctgttttccatcaacaatgagttctactacagtcgattgtttcctgatacttcaacttgaactggtggtctcgaagtccatcctaccgacatcagtcatacagtggtagaacggtcatctaccttcacaagactctcgacatgccctgcctgtgccgaagaggaggttggtcttagacaaaaggtagttgttactcaagatgatgtcagctaacaaaggactaBseRICLucTranslation 1991-333720740760780800820840860
    tcgtactttctctacttcagtgtaagagtcaacattcctgtgtgaaaccttcacatcccaagtgttccagaaacagtcggcggccataagaatctccttgcagggaccctggaattggtatcttgctttgtcaaatgtatcgtagaaagtatgtccagacaaaacgcaggttcctagcatgaaagagatgaagtcacattctcagttgtaaggacacactttggaagtgtagggttcacaaggtctttgtcagccgccggtattcttagaggaacgtccctgggaccttaaccatagaacgaaacagtttacatagcatctttcatacaggtctgttttgcgtccaaggaKflIPpuMICLucTranslation 1991-333NGS R8809009209409609801,0001,0201,040
    ctagtttcgggagcagcgcacgtctccctgtagtcaagcagcacgtgtgaggctcgctcgtctccacccatacagcgggcgaaggcgcaggagatggtgtagtagtagaagttgatggggttgcggcagctgtccttgtacggctccagaagacctttgcagtatgcaacgtcatgatcaaagccctcgtcgcgtgcagagggacatcagttcgtcgtgcacactccgagcgagcagaggtgggtatgtcgcccgcttccgcgtcctctaccacatcatcatcttcaactaccccaacgccgtcgacaggaacatgccgaggtcttctggaaacgtcatacgttgcagtaBplIBplIPmlIBmgBICLucTranslation 1991-333P3-FNGS F1,0601,0801,1001,1201,1401,1601,1801,2001,220
    caggatttccatagagtggacaaccgtcaaactcctggttgatcttaggctgaatagcgagttgttctggatctgaagtgaagtctgtatcttccatcattttaagatctccacagaggccagagatcattcctttgtttgctgtgtctggggcgattcttacagatcttcctccgtcctaaaggtatctcacctgttggcagtttgaggaccaactagaatccgacttatcgctcaacaagacctagacttcacttcagacatagaaggtagtaaaattctagaggtgtctccggtctctagtaaggaaacaaacgacacagaccccgctaagaatgtctagaaggaggPshAICLucTranslation 1991-333Guide opt 5Limi...lap1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gaggatgtcgatcacgatcagtttgaagaactcaatgacggtgatgttgaagcctggcatctcaacaacagcgatggtgacctcgccgatggtgtacgggttggcgatgatagggtcagctccaccgtttacagtgatgggattctcagtagcttgagcgatgtcaatgatgtctctcctacagctagtgctagtcaaacttcttgagttactgccactacaacttcggaccgtagagttgttgtcgctaccactggagcggctaccacatgcccaaccgctactatcccagtcgaggtggcaaatgtcactaccctaagagtcatcgaactcgctacagttactacagaBstEIICLucTranslation 1991-333Limited overlapLimited overlapGuide opt 1Guide opt 2Limited overlapR primer 2P1-RP2-F1,4201,4401,4601,4801,5001,5201,5401,560
    ccagccacttccagtcttgtcttggtcagcacagcccccttggttccatccaggttctccagggtgatggacaccttccagtcgccgcccttggttccttgacccaacacgtatgtaccaggttcctggaactggaatctctttccatagaatgttctaaaccatcctgcggcctggtcggtgaaggtcagaacagaaccagtcgtgtcgggggaaccaaggtaggtccaagaggtcccactacctgtggaaggtcagcggcgggaaccaaggaactgggttgtgcatacatggtccaaggaccttgaccttagagaaaggtatcttacaagatttggtaggacgccggaBaeIPfoIXcmIBstXIBstXISexAICLucTranslation 1991-333Guide opt 3Guide opt 4Limited overlapLimi...lapF ...21,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctactctgcattcaattacatactgacacattcggcaacatgtttttcctggtttattttcacacagtccatctgatagtatgtctctcgtgcaggtgccacagctgctatcaatacattctccttctttagcttcacaggaagttggaactgtgtttggtggatcaggttcgtagatgagacgtaagttaatgtatgactgtgtaagccgttgtacaaaaaggaccaaataaaagtgtgtcaggtagactatcatacagagagcacgtccacggtgtcgacgatagttatgtaagaggaagaaatcgaagtgtccttcaaccttgacacaaaccacctagtccaagcatPaqCICLucTranslation 1991-333APOBEC stop 11APOBEC stop 10APOBEC stop 9APOBEC stop 8APOBEC stop 7APOBEC stop 6APOBEC stop 5guide 6guide 5guide 4guide 3guide 2APOBEC stop 4guide 1APOBEC stop 3APOBEC stop 21,7601,7801,8001,8201,8401,8601,8801,9001,920
    aggacagtcctggcaatgaacagtggcgcagtagactaatgcaacggcaagaattaaggtcttcatggtggcggtaccaagcttgggtctccctatagtgagtcgtattaatttcgataagccagtaagcagtgggttctctagttagccagagagctctgcttatatagacctctcctgtcaggaccgttacttgtcaccgcgtcatctgattacgttgccgttcttaattccagaagtaccaccgccatggttcgaacccagagggatatcactcagcataattaaagctattcggtcattcgtcacccaagagatcaatcggtctctcgagacgaatatatctggagAccIBsaIKpnIAcc65IHindIIICLucTranslation 1991-333PCMVAPOBEC stop 1guide seedF primer 1T71,9401,9601,9802,0002,0202,0402,0602,0802,100
    ccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaacggtggcatgtgcggatggcgggtaaacgcagttaccccgcctcaacaatgctgtaaaacctttcagggcaactaaaaccacggttttgtttgagggtaactgcagttaccccacctctgaacctttaggggcactcagtttggcgataggtgcgggtaactacatgacggttttgCspCIPCMV2,1202,1402,1602,1802,2002,2202,2402,260
    cgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgcgtagtggtaccattatcgctactgattatgcatctacatgacggttcatcctttcagggtattccagtacatgacccgtattacggtccgcccggtaaatggcagtaactgcagttatcccccgcatgaaccgtatactatgtgaactacatgacggttcacccgtcaaatggNdeISnaBIPCMV2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gtaaatagtccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgcggaactccatatatgggctatgaactaatgaccccgtcatttatcaggtgggtaactgcagttacctttcagggataaccgcaatgatacccttgtatgcagtaataactgcagttacccgcccccagcaacccgccagtcggtccgcccggtaaatggcattcaatacattgcgccttgaggtatatacccgatacttgattactggggcaPCMV2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aattgattactattaataactagtcaataatcaatgtcaacgcgtatatctggcccgtacatcgAAGCTGAtgcaaagatggataaagttttaaacagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcattaactaatgataattattgatcagttattagttacagttgcgcatatagaccgggcatgtagcTTCGACTacgtttctacctatttcaaaatttgtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtBcgIMluISpeIEF-1aPCMVPD1712 bindingGH2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttcctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagAgeIMfeIEcoNIEF-1a2,8202,8402,8602,8802,9002,9202,9402,960
    tttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggcctaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggaacgcacggaacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccaaccttcacccaccctctcaagctccggaPstIStuIEF-1a2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggaagcgcggacagagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgctgcgaaaaaaagaccgttctatcagStuIAflIIEF-1a3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgtacaagccgctccgccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccggccggacgagaccacggaccggaApaIFseINaeINgoMIVPspOMIEF-1a3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccggcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggccgggacgacgtccctcgagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgtttccttttcccggaaaggcPasIPstIEcoNIBstAPIEF-1a3,5203,5403,5603,5803,6003,6203,6403,660
    tcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgaaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcagcagaaatccaacccccctccccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatccggtcgaaccgtgaactKasINarISfoITliIXhoIBbvCIPluTIBpu10IDraIIIPaeR7IEF-1a3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgacGTACGggatcctctagagccaccatgGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaaggtaaagtccacagcactgCATGCcctaggagatctcggtggtacCCTCAGTTTCAAGACAAACGGGACTAGACGTAGCGACACCGGCTAloIXbaIBamHIBsiWIEF-1aG-luciferaseTranslation 3979-4533XbaI GHCas13b-1C2c2-1PD4581PD27573,8603,8803,9003,9203,9403,9603,9804,0004,020
    GGCCAAGCCCACCGAGAACAACGAAGACTTCAACATCGTGGCCGTGGCCAGCAACTTCGCGACCACGGATCTCGATGCTGACCGCGGGAAGTTGCCCGGCAAGAAGCTGCCGCTGGAGGTGCTCAAAGAGATGGAAGCCAATGCCCGGAAAGCTGGCTGCACCAGGGGCTGTCTGCCGGTTCGGGTGGCTCTTGTTGCTTCTGAAGTTGTAGCACCGGCACCGGTCGTTGAAGCGCTGGTGCCTAGAGCTACGACTGGCGCCCTTCAACGGGCCGTTCTTCGACGGCGACCTCCACGAGTTTCTCTACCTTCGGTTACGGGCCTTTCGACCGACGTGGTCCCCGACAGACMscINruINmeAIIIG-luciferaseTranslation 3979-4533Semi degenerate oligo (b)Semi degenerate oligo (a/c)guide 46-guide-215 guide 2Canonical guide 2Canonical-2g1C2c2-1GHAnnealFP1PD2758AnnealGH4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ATCTGCCTGTCCCACATCAAGTGCACGCCCAAGATGAAGAAGTTCATCCCAGGACGCTGCCACACCTACGAAGGCGACAAAGAGTCCGCAcagGGCGGCATAGGCGAGGCGATCGTCGACATTCCTGAGATTCCTGGGTTCAAGGACTTGGAGCCCATGGAGCAGTTCATCGCACTAGACGGACAGGGTGTAGTTCACGTGCGGGTTCTACTTCTTCAAGTAGGGTCCTGCGACGGTGTGGATGCTTCCGCTGTTTCTCAGGCGTgtcCCGCCGTATCCGCTCCGCTAGCAGCTGTAAGGACTCTAAGGACCCAAGTTCCTGAACCTCGGGTACCTCGTCAAGTAGCGTGAccIAhdISalIG-luciferaseTranslation 3979-4533C-U Reporter mutagenesis site 215 guide 5Canonical-5Canonical guide 5g2C2c2-2GH4,2204,2404,2604,2804,3004,3204,3404,360
    AGGTCGATCTGTGTGTGGACTGCACAACTGGCTGCCTCAAAGGGCTTGCCAACGTGCAGTGTTCTGACCTGCTCAAGAAGTGGCTGCCGCAACGCTGTGCGACCTTTGCCAGCAAGATCCAGGGCCAGGTGGACAAGATCAAGGGGGCCGGTGGTGACTAAAATAAAATATCTTTTCCAGCTAGACACACACCTGACGTGTTGACCGACGGAGTTTCCCGAACGGTTGCACGTCACAAGACTGGACGAGTTCTTCACCGACGGCGTTGCGACACGCTGGAAACGGTCGTTCTAGGTCCCGGTCCACCTGTTCTAGTTCCCCCGGCCACCACTGATTTTATTTTATAGAAAG-luciferaseTranslation 3979-4533C-U Reporter mutagenesis site 2Gibson RPD4582Poly...nal4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ATTTTCATTACATCTGTGTGTTGGTTTTTTGTGTGcccgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatTAAAAGTAATGTAGACACACAACCAAAAAACACACgggcccatggctcgagcttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctaKpnISmaIXmaIEcoRITspMIAcc65IPoly A SignalEcoRI GH4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggaFspIKasINarINdeISfoIPluTIBstAPIpBR322pBR3224,7404,7604,7804,8004,8204,8404,8604,8804,900
    gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggctgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccDrdIPfoIpBR322pBR3224,9204,9404,9604,9805,0005,0205,0405,060
    tggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcaaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtSspIBciVIAmpR promoterTranslation 5203-5271pBR3225,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctataaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgataTranslation 5272-6063Transla...3-5271pBR3225,2605,2805,3005,3205,3405,3605,3805,4005,420
    gtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccgBcgIScaITranslation 5272-6063pBR3225,4405,4605,4805,5005,5205,5405,5605,5805,600
    caacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactagttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgatFspITranslation 5272-6063pBR3225,6205,6405,6605,6805,7005,7205,7405,760
    ttaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagaattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtcBsaINmeAIIITranslation 5272-6063pBR3225,7805,8005,8205,8405,8605,8805,9005,9205,940
    atggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaataccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattAhdITranslation 5272-6063pBR3225,9605,9806,0006,0206,0406,0606,0806,1006,120
    aaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtgttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccacpBR3226,1406,1606,1806,2006,2206,2406,2606,2806,300
    gtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgcaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacpBR3226,3206,3406,3606,3806,4006,4206,4406,460
    ccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggctpBR3226,4806,5006,5206,5406,5606,5806,6006,6206,640
    agggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaactccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgDrdIBciVIpBR3226,6606,6806,7006,7206,7406,7606,7806,8006,820
    gccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgccggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgpBR3226,8406,8606,8806,9006,9206,940
    • Plasmid
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    Editing disabled on read-only sequence.
    6957 bp500055006000650050010001500CLucTranslation 1991-333Guide opt 4Translation 5272-6063Limited overlapLimited overlapEco81IpBR322

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    6957