Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP
  • Sequence Map
    Editing disabled on read-only sequence.
    tgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccagAhdIBsaIT286*K284T*282GY280NG278SE276GD274HL272YS270RY268PL266A*264WR262AG260TS258CH256YR254SV252WA250*R248SW246I*244*C242YV240LW238GS236PG234LA232ST230T1-9240: Region 1LSM253Amp20406080100120140160
    ctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtgacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaFspIR228CS226*G224GG222DL220RN218IT216AP214FS212SY210TT208RW206NI204TQ202AV200NN198GN196SC194AD192HH190*A188RR186KT184HS182*E180AG178TG176LS174*P172SN1701-9240: Region 1LSM253Amp180200220240260280300320340
    tacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcScaICS168GG166HQ164AF162FR160NA158GE156TR154RD152ND150ST148LQ146GC144H*142*E140HN138HC136CQ134MI132RK130SD128HW126GY124SA122KR120HS118TL116V*114GL1121-9240: Region 1LSM253Amp360380400420440460480500520
    tcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagXmnI*E110SF108LT106HP104SR102TA100RA98RR96*Y94PI92IG90RW88MA86SS84*F82HE80DN78SF76TR74RP72SF70E*68PD66*R64QQ62SG60TR58HL56GS541-9240: Region 1LSM253Amp540560580600620640660680700
    gtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcTC52GV50SR48*C46RK44SE42GA40NR38PS36CF34CS32PL30IG28CF26LS24YP22RC20PF18TS16YE14D1-9240: Region 1LSM253Amp720740760780800820840860
    cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttatagcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatPsiICspCILSM2538809009209409609801,0001,0201,040
    aatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgAloILSM2531,0601,0801,1001,1201,1401,1601,1801,2001,220
    actaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcLSM2531,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcctccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcgcggaFspILSM2531,4201,4401,4601,4801,5001,5201,5401,560
    cgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattgtgacgtacgttaaagataatcatgtgtaaaattggcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacactgcatgcaatttctattagtacacattttaacBsiWI5' PB ITR Long5' PB ITR shortjt1009LSM2531,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacaaaaactcaaaatttcttctataaagtaacaaaatgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgtttttgagttttaaagaagatatttcattgtttt5' PB ITR LongLSM2531,7601,7801,8001,8201,8401,8601,8801,9001,920
    cttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcggaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcSmaISmaISrfIXmaIXmaIRsrIITspMITspMIcore insulLSM2531,9401,9601,9802,0002,0202,0402,0602,0802,100
    ctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccactagtTTCGCACGATTGCACCTTGGGAGCCTTAACCGCTTTTGAATGTCCGTCTTAGTTAATCCCTAATTGAACGCTCCAAAAGGTGACTTCCAATAgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgatcaAAGCGTGCTAACGTGGAACCCTCGGAATTGGCGAAAACTTACAGGCAGAATCAATTAGGGATTAACTTGCGAGGTTTTCCACTGAAGGTTATSfiISpeIattD Cp36core insulForward Targettarget ...primerLSM2532,1202,1402,1602,1802,2002,2202,2402,260
    GGGATTTATTCCTTTTAAAATTAACGGCATAATCGTGCCGCCGAATGCAACCTCGATCGAGCCGGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACCCCTAAATAAGGAAAATTTTAATTGCCGTATTAGCACGGCGGCTTACGTTGGAGCTAGCTCGGCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGBsmIMfeIEcoNIEF-1α promoterattD Cp36Primer 1Reverse TargetTarget ...Primerprime...F-1aLSM2532,2802,3002,3202,3402,3602,3802,4002,4202,440
    TGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAASacIIEF-1α promoterLSM2532,4602,4802,5002,5202,5402,5602,5802,6002,620
    GCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAXmnIAflIIEF-1α promoterLSM2532,6402,6602,6802,7002,7202,7402,7602,7802,800
    CGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTAApaIPciIBglIISacIIPspOMIEF-1α promoterLSM2532,8202,8402,8602,8802,9002,9202,9402,960
    GTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCFseIBstAPIEF-1α promoterLSM2532,9803,0003,0203,0403,0603,0803,1003,1203,140
    CCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCBbvCIEcoNIBpu10IEF-1α promoterfrag1LSM2533,1603,1803,2003,2203,2403,2603,2803,3003,320
    TCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGEF-1α promoterfrag1RF from EF-1aLSM2533,3403,3603,3803,4003,4203,4403,4603,4803,500
    CATTTCAGGTGTCGTGAATCTAGAAGCCACCatgTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTGTAAAGTCCACAGCACTTAGATCTTCGGTGGtacAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAXbaIXcmIBstEIILH2LD4QT6PS8RQ10PI12PS14EG16LQ18LH20LP22QV24LA26DA28VS30RL32VL34GK36FG38DL40TD42NF44SS46P3535-6975M1hADAR2 cat domainEF-1α...oterfrag1RF fr...F-1ahomologLSM2533,5203,5403,5603,5803,6003,6203,6403,660
    CACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGH48AR50RK52VL54AG56VV58MT60TG62TD64VK66DA68KV70IS72VS74TG76TK78CI80NG82EY84MS86DR88GL90AL92ND94CH96AE98II100SR102RS104L3535-6975hADAR2 cat domain3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGBsrGIL106RF108LY110TQ112LE114LY116LN118NK120DD122QK124RS126IF128QK130SE132RG134GF136RL138KE140NV142QF144HL146YI148ST150SP152CG154DA156RI158FS160PH162EP1643535-6975hADAR2 cat domain3,8603,8803,9003,9203,9403,9603,9804,0004,020
    AATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAAIL166EE168PA170DR172HP174NR176KA178RG180QL182RT184KI186ES188GQ190GT192IP194VR196SN198AS200IQ202AW204DG206VL208QG210ER212LL214TM216SC218SD220KI2223535-6975hADAR2 cat domainC-term ADAR24,0404,0604,0804,1004,1204,1404,1604,1804,200
    GCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGBlpIPpuMIAR224WN226VV228GI230QG232SL234LS236IF238VE240PI242YF244SS246II248LG250SL252YH254GD256HL258SR260AM262YQ264RI266SN268IE270DL272PP274LY276TL278NK2803535-6975hADAR2 cat domainC-term ADAR24,2204,2404,2604,2804,3004,3204,3404,360
    CTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTAMluIPL282LS284GI286SN288AE290AR292QP294GK296AP298NF300SV302NW304TV306GD308SA310IE312VI314NA316TT318GK320DE322LG324RA326SR328LC330KH332AL334YC336RW338M3535-6975hADAR2 cat domainC-term ADAR24,3804,4004,4204,4404,4604,4804,5004,5204,540
    GCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGR340VH342GK344VP346SH348LL350RS352KI354TK356PN358VY360HE362SK364LA366AK368EY370QA372AK374AR376LF378TA380FI382KA384GL386GA388WV390EK392PT394EQ396D3535-6975hADAR2 cat domainC-term ADAR24,5604,5804,6004,6204,6404,6604,6804,7004,720
    CAGTTCTCACTCACGCCCGGTACCGGTTCTGCAACCGGTgGATCCggcCTAGCTTCTAAAACAATAGTGTTGTCTGTAGGAGAAGCCACCAGAACCCTCACGGAGATCCAAAGCACAGCGGATAGACAAATTTTCGAAGAGAAAGTGGGACCGCTTGTCGGAAGACTCAGGTTGAGTCAAGAGTGAGTGCGGGCCATGGCCAAGACGTTGGCCAcCTAGGccgGATCGAAGATTTTGTTATCACAACAGACATCCTCTTCGGTGGTCTTGGGAGTGCCTCTAGGTTTCGTGTCGCCTATCTGTTTAAAAGCTTCTCTTTCACCCTGGCGAACAGCCTTCTGAGTCCAACTAhdIKpnIBstBIAcc65IQ398FS400LT402PG404TG406SA408TG410GS412GL414AS416KT418IV420LS422VG424EA426TR428TL430TE432IQ434ST436AD438RQ440IF442EE444KV446GP448LV450GR452LR454L3535-6975LSM098PCPshort linkerhADAR2...omainC-term ADAR24,7404,7604,7804,8004,8204,8404,8604,8804,900
    CAGCTTCCCTGCGGCAGAATGGCGCTAAGACCGCTTATAGGGTGAATCTCAAACTTGATCAAGCAGATGTTGTTGATAGCGGCTTGCCCAAAGTACGATATACTCAAGTTTGGTCACATGATGTGACAATCGTTGCAAACTCAACGGAAGCGAGTAGGAAAAGCCTTTATGATCTGTCGAAGGGACGCCGTCTTACCGCGATTCTGGCGAATATCCCACTTAGAGTTTGAACTAGTTCGTCTACAACAACTATCGCCGAACGGGTTTCATGCTATATGAGTTCAAACCAGTGTACTACACTGTTAGCAACGTTTGAGTTGCCTTCGCTCATCCTTTTCGGAAATACTAGAT456AS458LR460QN462GA464KT466AY468RV470NL472KL474DQ476AD478VV480DS482GL484PK486VR488YT490QV492WS494HD496VT498IV500AN502ST504EA506SR508KS510LY512DL5143535-6975LSM098PCP4,9204,9404,9604,9805,0005,0205,0405,060
    CACTAAGTCACTCGTCGCCACCTCTCAAGTTGAGGACCTTGTCGTCAATTTGGTGCCTCTTGGTCGGGGAGGCGGTTCCGGCGGGGGTACCCTTGCCAGTAAGACCATCGTGTTGAGCGTGGGAGAGGCAACCCGCACACTGACAGAAATCCAGTCAACAGCAGACAGACAGATTGTGATTCAGTGAGCAGCGGTGGAGAGTTCAACTCCTGGAACAGCAGTTAAACCACGGAGAACCAGCCCCTCCGCCAAGGCCGCCCCCATGGGAACGGTCATTCTGGTAGCACAACTCGCACCCTCTCCGTTGGGCGTGTGACTGTCTTTAGGTCAGTTGTCGTCTGTCTGTCTAAKpnIPpuMIAcc65ITK516SL518VA520TS522QV524ED526LV528VN530LV532PL534GR536GG538GS540GG542GT544LA546SK548TI550VL552SV554GE556AT558RT560LT562EI564QS566TA568DR570QI5723535-6975LSM098PCPPCP5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TTTGAGGAGAAAGTGGGTCCGCTTGTCGGGCGGCTGCGGCTGACGGCTTCATTGCGACAGAACGGAGCCAAAACTGCCTATCGAGTCAACCTTAAACTCGATCAAGCGGATGTTGTGGATTCCGGTCTGCCAAAAGTACGGTACACGCAGGTGTGGTCCCATGACGTGACCATTGAAACTCCTCTTTCACCCAGGCGAACAGCCCGCCGACGCCGACTGCCGAAGTAACGCTGTCTTGCCTCGGTTTTGACGGATAGCTCAGTTGGAATTTGAGCTAGTTCGCCTACAACACCTAAGGCCAGACGGTTTTCATGCCATGTGCGTCCACACCAGGGTACTGCACTGGTAACAleIBmgBIPaqCIFE574EK576VG578PL580VG582RL584RL586TA588SL590RQ592NG594AK596TA598YR600VN602LK604LD606QA608DV610VD612SG614LP616KV618RY620TQ622VW624SH626DV628TI6303535-6975LSM098PCP5,2605,2805,3005,3205,3405,3605,3805,4005,420
    TGGCGAACAGTACCGAGGCGTCTCGCAAAAGCCTTTACGATTTGACCAAATCACTCGTTGCAACTTCACAGGTGGAAGACCTGGTTGTCAATCTTGTACCTTTGGGCAGGGCTAGCGAAGGTCGGGGGAGTTTGCTTACCTGTGGCGATGTCGAGGAGAATCCAGGGCCCAAGCTACCGCTTGTCATGGCTCCGCAGAGCGTTTTCGGAAATGCTAAACTGGTTTAGTGAGCAACGTTGAAGTGTCCACCTTCTGGACCAACAGTTAGAACATGGAAACCCGTCCCGATCGCTTCCAGCCCCCTCAAACGAATGGACACCGCTACAGCTCCTCTTAGGTCCCGGGTTCGAApaIBmtINheIBsmBIEsp3ISexAIPspOMIHindIIIVA632NS634TE636AS638RK640SL642YD644LT646KS648LV650AT652SQ654VE656DL658VV660NL662VP664LG666RA668SE670GR672GS674LL676TC678GD680VE682EN684PG686PK688L3535-6975LSM098PCPT2ALSM2535,4405,4605,4805,5005,5205,5405,5605,5805,600
    TATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGATACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCBsiWIRsrIIBstEIIM690TE692YK694PT696VR698LA700TR702DD704VP706RA708VR710TL712AA714AF716AD718YP720AT722RH724TV726DP728DR730HI732ER734VT736EL738QE740LF742LT744RV746G3535-6975PuroRfrag3PR_S030PR_S029LSM2535,6205,6405,6605,6805,7005,7205,7405,760
    CTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGL748DI750GK752VW754VA756DD758GA760AV762AV764WT766TP768ES770VE772AG774AV776FA778EI780GP782RM784AE786LS788GS790RL792AA794QQ796QM798EG800LL802AP804H3535-6975PuroRfrag3LSM2535,7805,8005,8205,8405,8605,8805,9005,9205,940
    GGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCBsaIEagIR806PK808EP810AW812FL814AT816VG818VS820PD822HQ824GK826GL828GS830AV832VL834PG836VE838AA840ER842AG844VP846AF848LE850TS852AP854RN856LP858FY860ER862LG8643535-6975PuroRfrag3LSM2535,9605,9806,0006,0206,0406,0606,0806,1006,120
    CTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagagctgattaaggagaacatgGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctcgactaattcctcttgtacNcoISapIBmgBIBspQIPaqCISexAIBstAPIFT866VT868AD870VE872VP874EG876PR878TW880CM882TR884KP886GA888AT890NF892SL894LK896QA898GD900VE902EN904PG906PG908SM910VS912KG914EE916LI918KE920NM9223535-6975frag3PuroRP2Afrag4mtagBFP2homologoushomologLSM2536,1406,1606,1806,2006,2206,2406,2606,2806,300
    cacatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagagtgtacttcgacatgtacctcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctBsrGIHM924KL926YM928EG930TV932DN934HH936FK938CT940SE942GE944GK946PY948EG950TQ952TM954RI956KV958VE960GG962PL964PF966AF968DI970LA972TS974FL976YG978SK9803535-6975frag4mtagBFP2LSM2536,3206,3406,3606,3806,4006,4206,4406,460
    ccttcatcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttggaagtagttggtgtgggtcccgtaggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaaAloIBsu36ITF982IN984HT986QG988IP990DF992FK994QS996FP998EG1000FT1002WE1004RV1006TT1008YE1010DG1012GV1014LT1016AT1018QD1020TS1022LQ1024DG1026CL1028IY1030NV1032KI1034RG1036VN1038F3535-6975frag4mtagBFP2LSM2536,4806,5006,5206,5406,5606,5806,6006,6206,640
    cacatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctgtgtaggttgccgggacactacgtcttcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgaT1040SN1042GP1044VM1046QK1048KT1050LG1052WE1054AF1056TE1058TL1060YP1062AD1064GG1066LE1068GR1070ND1072MA1074LK1076LV1078GG1080SH1082LI1084AN1086AK1088TT1090YR1092SK1094KP1096A3535-6975frag4mtagBFP2LSM2536,6606,6806,7006,7206,7406,7606,7806,8006,820
    aagaacctcaagatgcctggcgtctactatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAGAATTCCTCGAGCTAGAGCTcGCTGttcttggagttctacggaccgcagatgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTCTTAAGGAGCTCGATCTCGAgCGACAccIPacIAlwNIEcoRIPspXIK1098NL1100KM1102PG1104VY1106YV1108DY1110RL1112ER1114IK1116EA1118NN1120ET1122YV1124EQ1126HE1128VA1130VA1132RY1134CD1136LP1138SK1140LG1142HK1144LN1146*3535-6975frag4mtagBFP2homologLSM2536,8406,8606,8806,9006,9206,9406,9606,9807,000
    ATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGbGH poly(A) signalLSM2537,0207,0407,0607,0807,1007,1207,1407,160
    AGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACGACGTCAGGTGGCACTTTTCgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataaTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTGCTGCAGTCCACCGTGAAAAGctatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattSphIEcoRV3' PB ITR longbGH poly(A) signalLSM2537,1807,2007,2207,2407,2607,2807,3007,3207,340
    tcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaatagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaBsmINsiISphI3' PB ITR long3'...tLSM2537,3607,3807,4007,4207,4407,4607,4807,5007,520
    tttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactga3' PB ITR long3' PB ITR shortjt1010LSM2537,5407,5607,5807,6007,6207,6407,6607,6807,700
    gctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttLSM2537,7207,7407,7607,7807,8007,8207,8407,860
    caataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaLSM2537,8807,9007,9207,9407,9607,9808,0008,0208,040
    ttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggtLSM2538,0608,0808,1008,1208,1408,1608,1808,2008,220
    acgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagtgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcPciISapIBspQILSM2538,2408,2608,2808,3008,3208,3408,3608,3808,400
    caaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgagttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctLSM2538,4208,4408,4608,4808,5008,5208,5408,560
    ccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaLSM2538,5808,6008,6208,6408,6608,6808,7008,7208,740
    tgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagAlwNILSM2538,7608,7808,8008,8208,8408,8608,8808,9008,920
    ggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccLSM2538,9408,9608,9809,0009,0209,0409,0609,0809,100
    tcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatcagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagL12A*10DT8LC6RD4*R2D1-9240: Region 2LSM253Amp9,1209,1409,1609,1809,2009,2209,240
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9240 bp65007000750080008500500100015002000LSM253attD Cp363' PB ITR shortjt1010Amp1-9240: Region 11-9240: Region 23535-6975XZp340.1 (DNA)oLSM0270 (DNA)oLSM0665 (DNA)EKo330.4 (DNA)XZ373-seq-R (DNA)XZp044.4 (DNA)XZp044.5 (DNA)pUC_oriR_Rv2 (DNA)oLSM-pUC_ori_F (DNA)oLSM-pUC_ori_R (DNA)pUC_oriR_Fv2 (DNA)AhdI,BsaI,FspIScaIXmnICspCI,PsiIAloIFspIjt1009,5' PB ITR short,BsiWI5' PB ITR Longcore insul,TspMI,XmaI,SmaI,RsrII,TspMI,XmaISmaI,SrfISfiISpeIForward Target,target forward primerAlwNIhomolog,PacI,EcoRI,PspXIbGH poly(A) signalSphIEcoRV3' PB ITR longBsmINsiISphIBspQI,SapIPciIAlwNI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    9240

    Welcome to Benchling!

    L
    Luis Santiago Mille Fragoso has shared a file with you.