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XZ039 CMV-TO-Shc1-MCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatggatctcctgccccccaagcccaagtacaatccactccggaatgagtctctgtcatcgctggaggaaggggcttctgggtccacccccccggaggagctgccttccccatcagcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacctagaggacggggggttcgggttcatgttaggtgaggccttactcagagacagtagcgacctccttccccgaagacccaggtgggggggcctcctcgacggaaggggtagtcgBbsIPmeIAflIIHindIIIMD2LL4PP6KP8KY10NP12LR14NE16SL18SS20LE22EG24AS26GS28TP30PE32EL34PS36PS38A760-3252p66shcCMV promot...th TetOs)homolog720740760780800820840860
    ttcatccctggggcccatcctgcctcctctgcctggggacgatagtcccactaccctgtgctccttcttcccccggatgagcaacctgaggctggccaacccggctggggggcgcccagggtctaagggggagccaggaagggcagctgatgatggggaggggatcgtaggggcaaagtagggaccccgggtaggacggaggagacggacccctgctatcagggtgatgggacacgaggaagaagggggcctactcgttggactccgaccggttgggccgaccccccgcgggtcccagattccccctcggtccttcccgtcgactactacccctcccctagcatccccgtKasINarIPasIPasISfoIPluTIS40SL42GP44IL46PP48LP50GD52DS54PT56TL58CS60FF62PR64MS66NL68RL70AN72PA74GG76RP78GS80KG82EP84GR86AA88DD90GE92GI94VG96A760-3252p66shc8809009209409609801,0001,0201,040
    gccatgccagactcaggccccctacccctcctccaggacatgaacaagctgagtggaggcggcgggcgcaggactcgggtggaagggggccagcttgggggcgaggagtggacccgccacgggagctttgtcaataagcccacgcggggctggctgcatcccaacgacaaagtcacggtacggtctgagtccgggggatggggaggaggtcctgtacttgttcgactcacctccgccgcccgcgtcctgagcccaccttcccccggtcgaacccccgctcctcacctgggcggtgccctcgaaacagttattcgggtgcgccccgaccgacgtagggttgctgtttcagtPfoICspCIEcoNIA98MP100DS102GP104LP106LL108QD110MN112KL114SG116GG118GR120RT122RV124EG126GQ128LG130GE132EW134TR136HG138SF140VN142KP144TR146GW148LH150PN152DK154V760-3252p66shc1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgggacccggggtttcctacttggttcggtacatgggttgtgtggaggtcctccagtcaatgcgtgccctggacttcaacacccggactcaggtcaccagggaggccatcagtctggtgtgtgaggctgtgccgggtgctaagggggcgacaaggaggagaaagccctgtagccgaccctgggccccaaaggatgaaccaagccatgtacccaacacacctccaggaggtcagttacgcacgggacctgaagttgtgggcctgagtccagtggtccctccggtagtcagaccacacactccgacacggcccacgattcccccgctgttcctcctctttcgggacatcggcBaeIKflIM156GP158GV160SY162LV164RY166MG168CV170EV172LQ174SM176RA178LD180FN182TR184TQ186VT188RE190AI192SL194VC196EA198VP200GA202KG204AT206RR208RK210PC212SR214760-3252p66shcPID domain1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cccgctcagctctatcctggggaggagtaacctgaaatttgctggaatgccaatcactctcaccgtctccaccagcagcctcaacctcatggccgcagactgcaaacagatcatcgccaaccaccacatgcaatctatctcatttgcatccggcggggatccggacacagccgaggggcgagtcgagataggacccctcctcattggactttaaacgaccttacggttagtgagagtggcagaggtggtcgtcggagttggagtaccggcgtctgacgtttgtctagtagcggttggtggtgtacgttagatagagtaaacgtaggccgcccctaggcctgtgtcggctcBsmIBamHIPL216SS218IL220GR222SN224LK226FA228GM230PI232TL234TV236ST238SS240LN242LM244AA246DC248KQ250II252AN254HH256MQ258SI260SF262AS264GG266DP268DT270AE272760-3252p66shcPID domain1,4201,4401,4601,4801,5001,5201,5401,560
    tatgtcgcctatgttgccaaagaccctgtgaatcagagagcctgccacattctggagtgtcccgaagggcttgcccaggatgtcatcagcaccattggccaggccttcgagttgcgcttcaaacaatacctcaggaacccacccaaactggtcacccctcatgacaggatggctgatacagcggatacaacggtttctgggacacttagtctctcggacggtgtaagacctcacagggcttcccgaacgggtcctacagtagtcgtggtaaccggtccggaagctcaacgcgaagtttgttatggagtccttgggtgggtttgaccagtggggagtactgtcctaccgacBcgIStuIPflMIYV274AY276VA278KD280PV282NQ284RA286CH288IL290EC292PE294GL296AQ298DV300IS302TI304GQ306AF308EL310RF312KQ314YL316RN318PP320KL322VT324PH326DR328MA330760-3252p66shcPID domain1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gctttgatggctcagcatgggatgaggaggaggaagagccacctgaccatcagtactataatgacttcccggggaaggaaccccccttggggggggtggtagacatgaggcttcgggaaggagccgctccaggggctgctcgacccactgcacccaatgcccagacccccagccacgaaactaccgagtcgtaccctactcctcctccttctcggtggactggtagtcatgatattactgaagggccccttccttggggggaaccccccccaccatctgtactccgaagcccttcctcggcgaggtccccgacgagctgggtgacgtgggttacgggtctgggggtcggtBsgIGF332DG334SA336WD338EE340EE342EP344PD346HQ348YY350ND352FP354GK356EP358PL360GG362VV364DM366RL368RE370GA372AP374GA376AR378PT380AP382NA384QT386PS388H760-3252p66shcPID domain1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cttgggagctacattgcctgtaggacagcctgttgggggagatccagaagtccgcaaacagatgccacctccaccaccctgtccaggcagagagctttttgatgatccctcctatgtcaacgtccagaacctagacaaggcccggcaagcagtgggtggtgctgggccccccaatgaaccctcgatgtaacggacatcctgtcggacaaccccctctaggtcttcaggcgtttgtctacggtggaggtggtgggacaggtccgtctctcgaaaaactactagggaggatacagttgcaggtcttggatctgttccgggccgttcgtcacccaccacgacccggggggttaEcoNIL390GA392TL394PV396GQ398PV400GG402DP404EV406RK408QM410PP412PP414PC416PG418RE420LF422DD424PS426YV428NV430QN432LD434KA436RQ438AV440GG442AG444PP446N760-3252p66shc1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cctgctatcaatggcagtgcaccccgggacctgtttgacatgaagcccttcgaagatgctcttcgcgtgcctccacctccccagtcggtgtccatggctgagcagctccgaggggagccctggttccatgggaagctgagccggcgggaggctgaggcactgctgcagctcaatgggacgatagttaccgtcacgtggggccctggacaaactgtacttcgggaagcttctacgagaagcgcacggaggtggaggggtcagccacaggtaccgactcgtcgaggctcccctcgggaccaaggtacccttcgactcggccgccctccgactccgtgacgacgtcgagttacAloIPstIXmnIBbvCIBstBIP448AI450NG452SA454PR456DL458FD460MK462PF464ED466AL468RV470PP472PP474QS476VS478MA480EQ482LR484GE486PW488FH490GK492LS494RR496EA498EA500LL502QL504N760-3252p66shcsh2 domain2,1202,1402,1602,1802,2002,2202,2402,260
    gggacttcctggtacgagagagcacgaccacacctggccagtatgtgctcactggcttgcagagtgggcagcctaagcatttgctactggtggaccctgagggtgtggttcggactaaggatcaccgctttgaaagtgtcagtcaccttatcagctaccacatggacaatcacttccctgaaggaccatgctctctcgtgctggtgtggaccggtcatacacgagtgaccgaacgtctcacccgtcggattcgtaaacgatgaccacctgggactcccacaccaagcctgattcctagtggcgaaactttcacagtcagtggaatagtcgatggtgtacctgttagtgaaG506DF508LV510RE512ST514TT516PG518QY520VL522TG524LQ526SG528QP530KH532LL534LV536DP538EG540VV542RT544KD546HR548FE550SV552SH554LI556SY558HM560DN562HL564760-3252p66shcsh2 domain2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gcccatcatctctgcgggcagcgaactgtgtctacagcaacctgtggagcggaaactgggcGGTGGTAGCGCTATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAcgggtagtagagacgcccgtcgcttgacacagatgtcgttggacacctcgcctttgacccgCCACCATCGCGATACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATAfeIPsrIPI566IS568AG570SE572LC574LQ576QP578VE580RK582LG584GG586SA588MA590SN592FT594QF596VL598VD600NG602GT604GD606VT608VA610PS612NF614AN616GI618AE620WI622760-3252MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPp66shcsh2 domainhomolog2,4602,4802,5002,5202,5402,5602,5802,6002,620
    AGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACXcmISS624NS626RS628QA630YK632VT634CS636VR638QS640SA642QN644RK646YT648IK650VE652VP654KG656AW658RS660YL662NM664EL666TI668PI670FA672TN674SD676CE678LI680760-3252MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAAgeIAsiSIVK682AM684QG686LL688KD690GN692PI694PS696AI698AA700NS702GI704YG706GG708TG710GG712SM714AS716NF718TQ720FV722LV724DN726GG728TG730DV732TV734AP736SN738F760-3252from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPMCP2,8202,8402,8602,8802,9002,9202,9402,960
    TGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCBplIBplIBstZ17IA740NG742IA744EW746IS748SN750SR752SQ754AY756KV758TC760SV762RQ764SS766AQ768NR770KY772TI774KV776EV778PK780GA782WR784SY786LN788ME790LT792IP794IF796A760-3252from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGAATTTACtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGCCTTAAATGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagTliIXhoIPspXIPaeR7I*1H1H2H3T798NS800DC802EL804IV806KA808MQ810GL812LK814DG816NP818IP820SA822IA824AN826SG828IY830*760-3252from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPc-myc epitopePo...g3,1603,1803,2003,2203,2403,2603,2803,3003,320
    atcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataBclIPmeIH4H5H6BGH polyadenylation sequence3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcBbsIClaISphIBspDIBGH polyadenylation sequenceBGH pA3,5203,5403,5603,5803,6003,6203,6403,660
    ccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttaggggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcKflI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagBarIXbaIHygromycinFRT3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattgaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaacHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcacccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgtPstIAsiSIBfuAIBspMIEcoRIRsrIIDraIIIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    aggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcgtccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcNdeIDraIIIHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgAjuIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgSacIIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctcccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggagPfoIHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    cagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtatacgtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatgBsmIPsiIBstZ17ISV40 pA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttgcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaa5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgt5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacccttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggPciIAflIIIpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    aggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacctccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggpUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatctpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    aggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggtcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccAcuIpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggataAmp (bla)6,3206,3406,3606,3806,4006,4206,4406,460
    ctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcgagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgAhdIBsaIAmp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    agaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgattcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctaAclIFspIAmp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    caaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatAmp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ctcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatggagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacAclIBcgIXmnIAmp (bla)7,0207,0407,0607,0807,1007,1207,1407,160
    taacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataaAcuISspIAmp (bla)bla promoter7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgcagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacSgrDIbla promoter7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWIAflIII7,5407,5607,5807,6007,6207,6407,660
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7668 bp550060006500700050010001500p66shcPID domainCMVTetR binding sitesTetO760-3252XZp039.1 (DNA)EKbF (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA)EK_CMVFa (DNA),EK_CMVFb (DNA),EK_CMVFc (DNA),EK_CMVFd (DNA),EKo272.1 (DNA),SpeI,+1CMV promoter (with TetOs),A,EK_CMVFe (DNA),SnaBI,CspCITATA box,TetO,CMV-Forward Genewiz (DNA),Eco53kI,SacI,SgrDI,Eco53kI,+1homolog,Kozak,start,CMVF1 (DNA),ToFlpF (DNA),XZp025.1 (DNA),+4PasIKasI,NarI,SfoI,PluTI,PasIEcoNI,PfoI,CspCIshc-seq-F1 (DNA)KflIBaeIBsmIBamHIPflMIBcgIshc-seq-F2 (DNA),StuIBsgIpUCoriR (DNA)pUC oriAcuIAhdIBsaIFspI,AclIAmp (bla)BcgI,AclI,XmnIAcuI,SspIbla promoter,FlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo152.4 (DNA),+6

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    7668

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