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EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMV20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcBmrICMV180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaNcoICspCISnaBICMV360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBIBsmBIEsp3ISgrDIEco53kIEco53kICMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgTACGAGTGTAATGAGTGCGGTAAGACCTTCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacATGCTCACATTACTCACGCCATTCTGGAAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAAPmeIAflIICspCIHindIIIM1YE2CN4EC6GK8TF10TR12SS14NL16IV18HQ20RI22HT24GE26KP28FA30CN32DC34GK36AF38763-1098ZNF35 ZF Domains 5-8720740760780800820840860
    TACTCAGAGTGCCAACTTGATCGTGCATCAGAGAAGCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGATGAGTCTCACGGTTGAACTAGCACGTAGTCTCTTCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCStuIStuIBsrGITQ40SA42NL44IV46HQ48RS50HT52GE54KP56YE58CK60EC62GK64AF66SC68FS70HL72IV74HQ76RI78HT80AE82KP84YD86CS88EC90GK92AF94SQ96763-1098ZNF35 ZF Domains 5-88809009209409609801,0001,0201,040
    CTGTCTTGCTTGATTGTGCATCAGAGAATTCACACGGGCGAACGCCCCTATATGTGCGTAGAGTGCGGTAAATGTTTTGGGCGGAGTTCACATCTTTTGCAGCACCAACGTATTCACACAGGCGAGAAACCCTATGTTTGCAGCGTGTGCGGTAAGGCGTTTAGCCAGTCCTCAGGACAGAACGAACTAACACGTAGTCTCTTAAGTGTGCCCGCTTGCGGGGATATACACGCATCTCACGCCATTTACAAAACCCGCCTCAAGTGTAGAAAACGTCGTGGTTGCATAAGTGTGTCCGCTCTTTGGGATACAAACGTCGCACACGCCATTCCGCAAATCGGTCAGGAGTCLS98CL100IV102HQ104RI106HT108GE110RP112763-1098YM2CV4EC6GK8CF10GR12SS14HL16LQ18HQ20RI22HT24GE26KP28YV30CS32VC34GK36AF38SQ40SS421099-1419ZNF250 ZF Domains 1-4ZNF35 ZF Domains 5-8Cano...ker1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TCCTTTCCAAACACCGCCGCATACATACTGGTGAGAAGCCATATGAATGTAATGAATGTGGCAAGGCTTTTAGAGTGAGTTCAGATCTCGCTCAACATCATAAGATCCATACAGGAGAGAAGCCACACGAATGTTTGGAGTGTCGAAAGGCTTTTACTCAGCTCAGTCACCTCATAGGAAAGGTTTGTGGCGGCGTATGTATGACCACTCTTCGGTATACTTACATTACTTACACCGTTCCGAAAATCTCACTCAAGTCTAGAGCGAGTTGTAGTATTCTAGGTATGTCCTCTCTTCGGTGTGCTTACAAACCTCACAGCTTTCCGAAAATGAGTCGAGTCAGTGGAGTAVL44SK46HR48RI50HT52GE54KP56YE58CN60EC62GK64AF66RV68SS70DL72AQ74HH76KI78HT80GE82KP84HE86CL88EC90RK92AF94TQ96LS98HL100I1099-1419ZNF250 ZF Domains 1-41,2401,2601,2801,3001,3201,3401,3601,3801,400
    TCAGCATCAGAGAATTCACGCTGCTGCATGGGAGCTAggaATGtctggcggatctTGCGTGACCTTTGAAGACATTGCCATCTATTTTAGTCAAGACGAATGGGGGCTCCTGGACGAAGCCCAGAGACTTCTGTACCTGGAGGTGATGCTTGAAAACTTCGCTCTCGTGGCATCCAGTCGTAGTCTCTTAAGTGCGACGACGTACCCTCGATcctTACagaccgcctagaACGCACTGGAAACTTCTGTAACGGTAGATAAAATCAGTTCTGCTTACCCCCGAGGACCTGCTTCGGGTCTCTGAAGACATGGACCTCCACTACGAACTTTTGAAGCGAGAGCACCGTAGGBaeIPfoIA1A2A3W4E5L6G7M8S91420-1446C1V2T3F4E5D6I7A8I9Y10F11S12Q13D14E15W16G17L18L19D20E21A22Q23R24L25L26Y27L28E29V30M31L32E33N34F35A36L37V38A39S401456-1581G1G2S3Q102HQ104RI106H1099-1419ZIK1 KRAB DomainZNF250...s 1-41,4201,4401,4601,4801,5001,5201,5401,560
    CTGtagggatcataggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttGACatccctagtatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaApaIAvaIBclIPmeITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMIEcoO109IL41*42BGH polyadenylation sequencec-myc epitopePolyhi...e tag1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctNspISphIBGH polyadenylation sequence1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaClaIBspDIBGH pA1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctcaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagNaeIXbaINgoMIVFRT2,1202,1402,1602,1802,2002,2202,2402,260
    tagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaaatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttBarIMscIBsaXIBsmBIEsp3IPflFIPshAITth111IHygromycinFRT2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggBfuAIBspMIDraIIIHygromycin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgcNcoIPstIPvuIAsiSIRsrIIHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    acaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccatgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggtPflFIDraIIINmeAIIITth111IHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    atacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgattatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctaAjuISacIIHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcctacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgScaIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    acgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcattgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtaNaeIPfoIPsiINgoMIVSV40 pA3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacgtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgBstZ17ISV40 pA3,5203,5403,5603,5803,6003,6203,6403,660
    atacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcSapIBsaXIBspQI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaNspIPciIAflIIIpUC ori3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcpUC ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccacgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtgAlwNIpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttagcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatBspHIpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctgttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagacAhdIBmrIAmp (bla)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagcggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcBsaINmeAIIIAmp (bla)4,9204,9404,9604,9805,0005,0205,0405,060
    tttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggAclIFspIPvuIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatacgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatBcgIScaIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacgAclIXmnIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggaSspIBspHIbla promoterAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    gacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatctgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtaMfeIAlwNISgrDI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgccttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMluIBsiWIAflIIIBpu10I5,9605,9806,000
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6007 bp45005000550050010001500ZIK1 KRAB DomainCMVTetR binding sitesTetO1456-1581EKbR (DNA)pNSK053 (DNA)FrtF (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)SpeIBmrI,SnaBI,NcoI,CspCITATA box,TetO,CMV-Forward Genewiz (DNA),Eco53kI,SacICMVF1 (DNA),BsaBI,BsaBI,SgrDI,Eco53kI,SacI,BsmBI,Esp3IKozak,start codon,760-762,ToFlpF (DNA),EKbF (DNA),+4CspCIZNF35 ZF Domains 5-8,763-1098,StuI,BsrGIStuICanonical ZF LinkerEWo100.2 (DNA),EWo100.3 (DNA)ZNF250 ZF Domains 1-41099-14191420-14461447-1455,EWo270.1 (DNA),EWo416 (DNA)BspHIAhdIBmrI,BsaINmeAIIIFspIAclIAmp (bla),PvuIScaI,BcgIAclI,XmnIbla promoter,SspI,BspHIFlpInF1 (DNA),FlpInF (DNA),SgrDI,AlwNI,MfeI,Bpu10I,BsiWI,AflIII,MluI

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    6007