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EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF
  • Sequence Map
    Editing disabled on read-only sequence.
    aagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacAmp20406080100120140160
    cttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtAhdIBsaIAmp180200220240260280300320340
    gccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaAclIFspIAmp360380400420440460480500520
    ccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacaAmp540560580600620640660680700
    gactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccctgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggAclIBcgIScaIXmnIAmp720740760780800820840860880
    agttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtccAmp9009209409609801,0001,0201,0401,060
    gttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccg1,0801,1001,1201,1401,1601,1801,2001,2201,240
    aaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccaAloIPsiI1,2601,2801,3001,3201,3401,3601,3801,4001,420
    gccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgcccggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcgg1,4401,4601,4801,5001,5201,5401,5601,5801,600
    gctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgccgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcgFspIBssHII1,6201,6401,6601,6801,7001,7201,7401,7601,780
    gcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattgtgacgtacgttaaagataatcatgtgtaaaattcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacactgcatgcaatttctattagtacacattttaaBsiWI31bp spacer5'TR(1)BII(R)5'TR1,8001,8201,8401,8601,8801,9001,9201,940
    gacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacaaaaactcaaaatttcttctataaagtaacaaaactctgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgtttttgagttttaaagaagatatttcattgttttga1,9601,9802,0002,0202,0402,0602,0802,1002,120
    tttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatcccagagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtagggtctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcgaaagSrfIcore insul2,1402,1602,1802,2002,2202,2402,2602,2802,300
    ctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgacgattgagtaacactgtatctagtctacctacagggctcATATATAAAGAGATATATAAAGAGctcttcATATATAAAGAGATATATAAAGAGgaggtgagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaactgctaactcattgtgacatagatcagatggatgtcccgagTATATATTTCTCTATATATTTCTCgagaagTATATATTTCTCTATATATTTCTCctccaMfeISfiIcore insulZNF35/ZNF35 siteZNF35/ZNF35 site2,3202,3402,3602,3802,4002,4202,4402,4602,480
    cATATATAAAGAGATATATAAAGAGcagaacATATATAAAGAGATATATAAAGAGtatcagtctagagggtatataatggatcccatcgcgtctcagcctcactttgagctcctccacacgaattccccgataccgtcgattcaaggagcttgcttgttctttttgcagaagctcagagTATATATTTCTCTATATATTTCTCgtcttgTATATATTTCTCTATATATTTCTCatagtcagatctcccatatattacctagggtagcgcagagtcggagtgaaactcgaggaggtgtgcttaaggggctatggcagctaagttcctcgaacgaacaagaaaaacgtcttcgagtctXbaIXenopus g...n 5'-UTRZNF35/ZNF35 siteZNF35/ZNF35 site2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ataaacgctcaactttggcagatctaccaagcttggtaccgagctcggatccaactcgaggccaccatgccagagccagcgaagtctgctcccgccccgaaaaagggctccaagaaggcggtgactaaggcgcagaagaaaggcggcaagaagcgcaagcgcagccgcaaggagagcttatttgcgagttgaaaccgtctagatggttcgaaccatggctcgagcctaggttgagctccggtggtacggtctcggtcgcttcagacgagggcggggctttttcccgaggttcttccgccactgattccgcgtcttctttccgccgttcttcgcgttcgcgtcggcgttcctctcgaKpnITliIXhoIBglIIPspXIAcc65IPaeR7IMP2EP4AK6SA8PA10PK12KG14SK16KA18VT20KA22QK24KG26GK28KR30KR32SR34KE36SH2B-BFPH2BXenopu...'-UTR2,6802,7002,7202,7402,7602,7802,8002,8202,840
    attccatctatgtgtacaaggttctgaagcaggtccaccctgacaccggcatttcgtccaaggccatgggcatcatgaattcgtttgtgaacgacattttcgagcgcatcgcaggtgaggcttcccgcctggcgcattacaacaagcgctcgaccatcacctccagggagatccagactaaggtagatacacatgttccaagacttcgtccaggtgggactgtggccgtaaagcaggttccggtacccgtagtacttaagcaaacacttgctgtaaaagctcgcgtagcgtccactccgaagggcggaccgcgtaatgttgttcgcgagctggtagtggaggtccctctaggtctgAfeIPaqCIY38SI40YV42YK44VL46KQ48VH50PD52TG54IS56SK58AM60GI62MN64SF66VN68DI70FE72RI74AG76EA78SR80LA82HY84NK86RS88TI90TS92RE94IQ96TH2B-BFPH2B2,8602,8802,9002,9202,9402,9602,9803,0003,020
    ggccgtgcgcctgctgctgcctggggagttggccaagcacgccgtgtccgagggtactaaggccatcaccaagtacaccagcgctaaggatccccgggtaccggtcgtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtggacaacccggcacgcggacgacgacggacccctcaaccggttcgtgcggcacaggctcccatgattccggtagtggttcatgtggtcgcgattcctaggggcccatggccagcacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcacctgttgAfeIAgeIKpnIAcc65IBpu10IA98VR100LL102LP104GE106LA108KH110AV112SE114GT116KA118IT120KY122TS124AK126DP128RV130PV132VS134KG136EE138LI140KE142NM144HM146KL148YM150EG152TV154DN156H2B-BFPH2BmtagBFP2linker3,0403,0603,0803,1003,1203,1403,1603,1803,200
    catcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatccccgacttcttcagtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaggggctgaagaagtHH158FK160CT162SE164GE166GK168PY170EG172TQ174TM176RI178KV180VE182GG184PL186PF188AF190DI192LA194TS196FL198YG200SK202TF204IN206HT208QG210IP212DF214FH2B-BFPmtagBFP23,2203,2403,2603,2803,3003,3203,3403,3603,380
    agcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagaagaaaacactcggctgtcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcttcttttgtgagccgacAloIPfoIBsu36IK216QS218FP220EG222FT224WE226RV228TT230YE232DG234GV236LT238AT240QD242TS244LQ246DG248CL250IY252NV254KI256RG258VN260FT262SN264GP266VM268QK270KT272LG274WH2B-BFPmtagBFP23,4003,4203,4403,4603,4803,5003,5203,5403,560
    ggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatgtggactacagactgcctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatgatacacctgatgtctgacE276AF278TE280TL282YP284AD286GG288LE290GR292ND294MA296LK298LV300GG302SH304LI306AN308AK310TT312YR314SK316KP318AK320NL322KM324PG326VY328YV330DY332RL334H2B-BFPmtagBFP23,5803,6003,6203,6403,6603,6803,7003,720
    gaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaagcttaattagctagcgaattcgaatttaaatcggcctcgactgtgccttctagttgccagccatctgttgtttgcccctccccctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgttcgaattaatcgatcgcttaagcttaaatttagccggagctgacacggaagatcaacggtcggtagacaacaaacggggaggggBmtINheISwaIBstBIER336IK338EA340NN342ET344YV346EQ348HE350VA352VA354RY356CD358LP360SK362LG364HK366LN368*H2B-BFPmtagBFP2BGH polyadenylation sequenceMCS3,7403,7603,7803,8003,8203,8403,8603,8803,900
    cgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaattgcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaNotIBGH polyadenylation sequence3,9203,9403,9603,9804,0004,0204,0404,0604,080
    taacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttXmnISexAIpSV405, 299 F4,1004,1204,1404,1604,1804,2004,2204,2404,260
    ccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaaggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttAvrIIpSV404,2804,3004,3204,3404,3604,3804,4004,4204,440
    gctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtcgagggccctcgaacatataggtaaaagcctagactagtcgtgcactactttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcaBclIPmlIPflFIPshAITth111IHygro4,4604,4804,5004,5204,5404,5604,5804,6004,620
    ggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgccctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacgHygro2, 563 F4,6404,6604,6804,7004,7204,7404,7604,7804,800
    ctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcagactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtNdeIAsiSIHygro4,8204,8404,8604,8804,9004,9204,9404,9604,980
    aactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagPflFITth111IHygro5,0005,0205,0405,0605,0805,1005,1205,1405,160
    ggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcacccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgtAjuISacIIHygro3, 135 F5,1805,2005,2205,2405,2605,2805,3005,3205,340
    atttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggataaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttcctScaIHygro6, 730 R5,3605,3805,4005,4205,4405,4605,4805,500
    ataggtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatatccagctgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttHpaIPsiIWPRESV40 pASV40 pA(1)5,5205,5405,5605,5805,6005,6205,6405,6605,680
    tttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttccaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaaggBsaISV40 pASV40 pA(1)5,7005,7205,7405,7605,7805,8005,8205,8405,860
    caaactgggttcttatatcccttgctctggtcaaccaggttgcagggtttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggtttgacccaagaatatagggaacgagaccagttggtccaacgtcccaaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtcApaIEcoNISexAIPspOMIcore insul(1...re insul(1)5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gctcaaagagcagcgagaagcgttcagaggaaagcgatcccgtgccaccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggcgagtttctcgtcgctcttcgcaagtctcctttcgctagggcacggtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccSrfIcore insul(1) Geneious name: core insul(1)6,0606,0806,1006,1206,1406,1606,1806,2006,220
    ggctttgggggggggctgtccctgatgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgccgaaacccccccccgacagggactacaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattacSpeICMVcore ins...nsul(1)6,2406,2606,2806,3006,3206,3406,3606,3806,400
    acgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttattgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataNdeICMV6,4206,4406,4606,4806,5006,5206,5406,5606,580
    gggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggSnaBICMV6,6006,6206,6406,6606,6806,7006,7206,7406,760
    aaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgtttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaBglIIBsaBIBsaBISgrDICMVTetR binding sitesTetOTetO6,7806,8006,8206,8406,8606,8806,9006,9206,940
    tttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgTACGAGTGTAATGAGTGCGGTAAGACCTTCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacATGCTCACATTACTCACGCCATTCTGGAAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACAPmeIAflIIMY2EC4NE6CG8KT10FT12RS14SN16LI18VH20QR22IH24TG26EK28PF30AC32ND34CZNF35/ZNF35 TFZNF35 ZF Domains 5-86,9606,9807,0007,0207,0407,0607,0807,1007,120
    GGGAAGGCTTTTACTCAGAGTGCCAACTTGATCGTGCATCAGAGAAGCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTCCCTTCCGAAAATGAGTCTCACGGTTGAACTAGCACGTAGTCTCTTCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAG36KA38FT40QS42AN44LI46VH48QR50SH52TG54EK56PY58EC60KE62CG64KA66FS68CF70SH72LI74VH76QR78IH80TA82EK84PY86DC88SE90CG92KA94ZNF35/ZNF35 TFZNF35 ZF Domains 5-87,1407,1607,1807,2007,2207,2407,2607,280
    TTAGTCAGCTGTCTTGCTTGATTGTGCATCAGAGAATTCACACGGGCGAACGCCCCTACGAGTGTAATGAGTGCGGTAAGACCTTCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTTACTCAGAGAATCAGTCGACAGAACGAACTAACACGTAGTCTCTTAAGTGTGCCCGCTTGCGGGGATGCTCACATTACTCACGCCATTCTGGAAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAAATGAGTCTCFS96QL98SC100LI102VH104QR106IH108TG110ER112PY114EC116NE118CG120KT122FT124RS126SN128LI130VH132QR134IH136TG138EK140PF142AC144ND146CG148KA150FT152QS154ZNF35/ZNF35 TFZNF35 ZF Domains 5-8ZNF35 ZF Domains 5-8Cano...ker7,3007,3207,3407,3607,3807,4007,4207,4407,460
    TGCCAACTTGATCGTGCATCAGAGAAGCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGCTGTCTTGCTTGACGGTTGAACTAGCACGTAGTCTCTTCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCGACAGAACGAACAN156LI158VH160QR162SH164TG166EK168PY170EC172KE174CG176KA178FS180CF182SH184LI186VH188QR190IH192TA194EK196PY198DC200SE202CG204KA206FS208QL210SC212LZNF35/ZNF35 TFZNF35 ZF Domains 5-87,4807,5007,5207,5407,5607,5807,6007,6207,640
    ATTGTGCATCAGAGAATTCACgctgctgcaTGGGAGCTAggaATGtctggcggatctGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAACCAGGTAACACGTAGTCTCTTAAGTGcgacgacgtACCCTCGATcctTACagaccgcctagaCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTGGTCCBlpIBsgII214VH216QR218IH220AA222AW224EL226GM228SG230GS232EG234QS236DE238RA240LL242DQ244LH246TL248LS250NT252DA254TG256LE258EI260DR262AL264GI266PE268LV270NQ272ZNF35/ZNF35 TFN (NCOA3) Activation DomainNZF DomainZNF35 Z...ns 5-87,6607,6807,7007,7207,7407,7607,7807,8007,820
    GCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCCTGCTGGACGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGGACGACCTBmgBIEcoNIPflMIPpuMIGQ274AL276EP278KQ280DG282GW284GS286MA288EE290FV292TL294KD296VG298MD300FT302LG304DW306EQ308LG310LE312QG314DT316FW318DT320AL322DN324CQ326DL328FL330LD332ZNF35/ZNF35 TFNZF DomainZ (ZNF473) Activation DomainN (NCOA3) ...on Domain7,8407,8607,8807,9007,9207,9407,9607,9808,000
    CCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCtaggccgctcgaggagggcccGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGatccggcgagctcctcccgggApaIPciITliIXhoIPspXIAflIIIPaeR7IPspOMIPP334GG336GD338GL340GL342GH344EK346FP348SD350LD352LD354MF356NG358SL360EC362DM364ES366II368RS370EL372MD374AD376GL378DF380NF382DS384*ZNF35/ZNF35 TFNZF DomainF (FOXO3) Activation Domain8,0208,0408,0608,0808,1008,1208,1408,1608,180
    gaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaBclIPmeIBGH polyadenylation sequencec-myc epitopePolyhi...e tag8,2008,2208,2408,2608,2808,3008,3208,3408,360
    cctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggaatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgaccccttagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttatBGH polyadenylation sequence8,3808,4008,4208,4408,4608,4808,5008,5208,540
    acaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgtgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagc8,5608,5808,6008,6208,6408,6608,6808,7008,720
    cgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaag3'TR8,7408,7608,7808,8008,8208,8408,8608,8808,900
    ccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacaggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgt8,9208,9408,9608,9809,0009,0209,0409,060
    cagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgBsgI9,0809,1009,1209,1409,1609,1809,2009,2209,240
    ccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctBssHIIpLac(F)9,2609,2809,3009,3209,3409,3609,3809,4009,420
    aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggatattggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctat9,4409,4609,4809,5009,5209,5409,5609,5809,600
    acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggtgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccPciIAflIII9,6209,6409,6609,6809,7009,7209,7409,7609,780
    aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgcttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcg9,8009,8209,8409,8609,8809,9009,9209,9409,960
    tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgc9,98010,00010,02010,04010,06010,08010,10010,12010,140
    ctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaggagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttc10,16010,18010,20010,22010,24010,260
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10275 bp75008000850090009500100005001000150020002500ZNF35/ZNF35 siteGibson overlapMluI-PGK-GFNew StopZNF35/ZNF35 TFEWo103.2 (DNA)EWo101.1 (DNA)EWo100.2 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),AhdI,BsaI,FspI,AclIAmp,ScaI,BcgIAclIXmnIPsiI,AloIFrtF (DNA)FlpInR (DNA)M13-40FOR Genewiz (DNA),FspIBII(R),M13F Genewiz (DNA),BssHII5'TRBsiWI31bp spacer,5'TR(1),5'TR,5'TRcore insul,EWXglobF NONSPECIFIC DONT USE (DNA),SrfIAdded MluI site,ZNF35/ZNF35 site,EWXglobF2 (DNA),+3ZNF35/ZNF35 siteZNF35/ZNF35 site,EWo145.1 (DNA),XbaIEWo196.1 (DNA),EWo196.2 (DNA)N (NCOA3) Activation Domain,EWo211 (DNA),BsgI,BlpIEW_NZF_R (DNA),PflMI,BmgBINZF Domain,Z (ZNF473) Activation Domain,EcoNI,PpuMIF (FOXO3) Activation Domain,AflIII,PciIc-myc epitope,Polyhistidine tag,STOP (missing now),+9BGH polyadenylation sequence,BGHR Genewiz (DNA),+1bghF (DNA)EWo370.2 (DNA)3'TRES003-3 C->ABsgI,BssHIIpLac(F),M13R Genewiz (DNA)pUCori5F (DNA)pUCoriR (DNA),AflIII,PciI

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    10275