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LSM044-3 CMV-TO-CCR7-ADAR2dd(E488Q, T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggpcr from lsm044-2pUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIAmp (bla)pcr from lsm044-2180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspINmeAIIIAmp (bla)pcr from lsm044-2360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp (bla)pcr from lsm044-2540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp (bla)pcr from lsm044-2720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoterpcr from lsm044-28809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactSgrDIbla promoterpcr from lsm044-21,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIAlwNIBsiWIBpu10Ipcr from lsm044-21,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)pcr from lsm044-21,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaNdeICMVCMV promoter (with TetOs)pcr from lsm044-21,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggCspCISnaBICMVCMV promoter (with TetOs)pcr from lsm044-21,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgSacISacIBsaBIBsaBIBsmBIEsp3ISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetOpcr from lsm044-21,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccATGGATCTCGGGAAGCCTATGAAAAGCGTACTGGTTGTGGCTTTGCTGGTGATATTCCAGGTATGTCTGTGTCAGGATGAAGTTACAGACGACTACATAGGCGACAATACCACAGTAGATTACgccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggTACCTAGAGCCCTTCGGATACTTTTCGCATGACCAACACCGAAACGACCACTATAAGGTCCATACAGACACAGTCCTACTTCAATGTCTGCTGATGTATCCGCTGTTATGGTGTCATCTAATGAflIIHindIIIMD2LG4KP6MK8SV10LV12VA14LL16VI18FQ20VC22LC24QD26EV28TD30DY32IG34DN36TT38VD40Y2153-4510from original LSM044 (digest same)Ccr7CMV pro...TetOs)homologpcr from lsm044-22,1202,1402,1602,1802,2002,2202,2402,260
    ACCCTTTTCGAGTCCCTCTGCAGCAAGAAGGACGTGAGGAATTTCAAGGCATGGTTTCTGCCTATTATGTATTCCATAATCTGCTTTGTCGGGCTGCTGGGGAACGGTTTGGTCGTCTTGACGTATATTTACTTCAAACGCCTGAAGACAATGACAGATACCTACCTTTTGAACCTGGGAAAAGCTCAGGGAGACGTCGTTCTTCCTGCACTCCTTAAAGTTCCGTACCAAAGACGGATAATACATAAGGTATTAGACGAAACAGCCCGACGACCCCTTGCCAAACCAGCAGAACTGCATATAAATGAAGTTTGCGGACTTCTGTTACTGTCTATGGATGGAAAACTTGGBarIBmgBIT42LF44ES46LC48SK50KD52VR54NF56KA58WF60LP62IM64YS66II68CF70VG72LL74GN76GL78VV80LT82YI84YF86KR88LK90TM92TD94TY96LL98N2153-4510from original LSM044 (digest same)Ccr7TM1pcr from lsm044-22,2802,3002,3202,3402,3602,3802,4002,4202,440
    TCGCCGTGGCCGATATTCTGTTTCTTCTCACTCTCCCTTTCTGGGCATATTCCGCAGCAAAGTCATGGGTCTTCGGAGTCCACTTTTGCAAGCTGATCTTTGCTATATACAAGATGAGCTTTTTCTCTGGGATGCTGCTGCTGCTGTGCATCAGCATAGATAGATATGTGGCCATAGCGGCACCGGCTATAAGACAAAGAAGAGTGAGAGGGAAAGACCCGTATAAGGCGTCGTTTCAGTACCCAGAAGCCTCAGGTGAAAACGTTCGACTAGAAACGATATATGTTCTACTCGAAAAAGAGACCCTACGACGACGACGACACGTAGTCGTATCTATCTATACACCGGTAL100AV102AD104IL106FL108LT110LP112FW114AY116SA118AK120SW122VF124GV126HF128CK130LI132FA134IY136KM138SF140FS142GM144LL146LL148CI150SI152DR154YV156AI1582153-4510from original LSM044 (digest same)Ccr7pcr from lsm044-22,4602,4802,5002,5202,5402,5602,5802,6002,620
    TGTGCAGGCGGTCTCTGCACATAGACACCGGGCTAGGGTGCTTCTGATAAGTAAATTGAGCTGCGTTGGGATCTGGATTTTGGCCACAGTCTTGTCCATTCCTGAACTGTTGTACTCAGATTTGCAGAGGAGTTCCTCTGAGCAAGCTATGCGATGTTCACTGATCACTGAGCATACACGTCCGCCAGAGACGTGTATCTGTGGCCCGATCCCACGAAGACTATTCATTTAACTCGACGCAACCCTAGACCTAAAACCGGTGTCAGAACAGGTAAGGACTTGACAACATGAGTCTAAACGTCTCCTCAAGGAGACTCGTTCGATACGCTACAAGTGACTAGTGACTCGTABsgIBsgIVQ160AV162SA164HR166HR168AR170VL172LI174SK176LS178CV180GI182WI184LA186TV188LS190IP192EL194LY196SD198LQ200RS202SS204EQ206AM208RC210SL212IT214EH2162153-4510from original LSM044 (digest same)Ccr7pcr from lsm044-22,6402,6602,6802,7002,7202,7402,7602,7802,800
    GTGGAAGCGTTCATTACGATCCAAGTCGCCCAAATGGTGATTGGCTTCCTTGTTCCTTTGCTTGCTATGAGCTTTTGCTACCTCGTAATTATACGCACACTCCTGCAGGCCCGGAATTTTGAGCGGAACAAGGCTATTAAGGTGATAATTGCCGTAGTGGTTGTATTCATTGTCTCACCTTCGCAAGTAATGCTAGGTTCAGCGGGTTTACCACTAACCGAAGGAACAAGGAAACGAACGATACTCGAAAACGATGGAGCATTAATATGCGTGTGAGGACGTCCGGGCCTTAAAACTCGCCTTGTTCCGATAATTCCACTATTAACGGCATCACCAACATAAGTAACAGASbfIXcmIVE218AF220IT222IQ224VA226QM228VI230GF232LV234PL236LA238MS240FC242YL244VI246IR248TL250LQ252AR254NF256ER258NK260AI262KV264II266AV268VV270VF272IV2742153-4510from original LSM044 (digest same)Ccr7pcr from lsm044-22,8202,8402,8602,8802,9002,9202,9402,960
    TTCAGCTGCCTTATAATGGGGTAGTTTTGGCCCAAACAGTTGCTAACTTTAACATCACTTCTTCAACATGTGAGCTGTCAAAGCAGCTGAATATCGCATACGATGTGACATACTCCTTGGCGTGCGTCAGGTGTTGCGTGAATCCTTTCCTGTACGCTTTTATTGGTGTGAAGTTAAGTCGACGGAATATTACCCCATCAAAACCGGGTTTGTCAACGATTGAAATTGTAGTGAAGAAGTTGTACACTCGACAGTTTCGTCGACTTATAGCGTATGCTACACTGTATGAGGAACCGCACGCAGTCCACAACGCACTTAGGAAAGGACATGCGAAAATAACCACACTTCAAPciIPsiIXmnIFQ276LP278YN280GV282VL284AQ286TV288AN290FN292IT294SS296TC298EL300SK302QL304NI306AY308DV310TY312SL314AC316VR318CC320VN322PF324LY326AF328IG330VK332F2153-4510from original LSM044 (digest same)Ccr7pcr from lsm044-22,9803,0003,0203,0403,0603,0803,1003,1203,140
    TCGAAATGACCTGTTTAAACTTTTCAAAGACTTGGGCTGTCTGTCTCAGGAGCAGCTTCGCCAGTGGTCATCATGCAGACACATTAGGCGCTCCAGTATGAGTGTGGAGGCAGAGACCACTACTACGTTCTCCCCTGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCAGCTTTACTGGACAAATTTGAAAAGTTTCTGAACCCGACAGACAGAGTCCTCGTCGAAGCGGTCACCAGTAGTACGTCTGTGTAATCCGCGAGGTCATACTCACACCTCCGTCTCTGGTGATGATGCAAGAGGGGACCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGAgeIKpnIXcmIBstBIAcc65IR334ND336LF338KL340FK342DL344GC346LS348QE350QL352RQ354WS356SC358RH360IR362RS364SM366SV368EA370ET372TT374TF376SP378GT380GA382LH384LD386QT388PS390R2153-4510from original LSM044 (digest same)Ccr7hADAR2 cat domainhomologpcr from lsm044-23,1603,1803,2003,2203,2403,2603,2803,3003,320
    CAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACBstEIIQ392PI394PS396EG398LQ400LH402LP404QV406LA408DA410VS412RL414VL416GK418FG420DL422TD424NF426SS428PH430AR432RK434VL436AG438VV440MT442TG444TD446VK448D2153-4510hADAR2 cat domainpcr from lsm044-23,3403,3603,3803,4003,4203,4403,4603,4803,500
    CCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGA450KV452IS454VS456TG458TK460CI462NG464EY466MS468DR470GL472AL474ND476CH478AE480II482SR484RS486LL488RF490LY492TQ494LE496LY498LN500NK502DD504QK506RS5082153-4510hADAR2 cat domainpcr from lsm044-23,5203,5403,5603,5803,6003,6203,6403,660
    CATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGPfoIBsrGIIF510QK512SE514RG516GF518RL520KE522NV524QF526HL528YI530ST532SP534CG536DA538RI540FS542PH544EP546IL548EE550PA552DR554HP556NR558KA560RG562QL564RT5662153-4510hADAR2 cat domainpcr from lsm044-23,6803,7003,7203,7403,7603,7803,8003,8203,840
    AAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAABcgIBlpIBamHIKI568ES570GQ572GT574IP576VR578SN580AS582IQ584AW586DG588VL590QG592ER594LL596TM598SC600SD602KI604AR606WN608VV610GI612QG614SL616LS618IF620VE622PI6242153-4510hADAR2 cat domainpcr from lsm044-23,8603,8803,9003,9203,9403,9603,9804,0004,020
    ACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCTliIXhoIPpuMIPaeR7IYF626SS628II630LG632SL634YH636GD638HL640SR642AM644YQ646RI648SN650IE652DL654PP656LY658TL660NK662PL664LS666GI668SN670AE672AR674QP676GK678AP680NF682S2153-4510hADAR2 cat domainpcr from lsm044-24,0404,0604,0804,1004,1204,1404,1604,1804,200
    TGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACMluIV684NW686TV688GD690SA692IE694VI696NA698TT700GK702DE704LG706RA708SR710LC712KH714AL716YC718RW720MR722VH724GK726VP728SH730LL732RS734KI736TK738PN740V2153-4510hADAR2 cat domainpcr from lsm044-24,2204,2404,2604,2804,3004,3204,3404,360
    TACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttcApaITliIXhoIPspXIBpu10IPaeR7IPspOMI*1Y742HE744SK746LA748AK750EY752QA754AK756AR758LF760TA762FI764KA766GL768GA770WV772EK774PT776EQ778DQ780FS782LT784P*7862153-4510hADAR2 cat domainc-myc epitopepcr from lsm044-24,3804,4004,4204,4404,4604,4804,5004,5204,540
    aggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaBGH polyadenylation sequencePolyhi...e tagc-...epcr from lsm044-24,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttctClaISphIBspDIBGH polyadenylation sequenceBGH pApcr from lsm044-24,7404,7604,7804,8004,8204,8404,8604,8804,900
    accagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagNaeINgoMIVpcr from lsm044-24,9204,9404,9604,9805,0005,0205,0405,060
    taaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagBarIKflIXbaIPpuMIPshAIFRTHygromycinpcr from lsm044-25,0805,1005,1205,1405,1605,1805,2005,2205,240
    gagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccBsmBIEsp3IHygromycinpcr from lsm044-25,2605,2805,3005,3205,3405,3605,3805,4005,420
    ccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcPvuIAsiSIEcoRIDraIIIHygromycinpcr from lsm044-25,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgagggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcNdeIRsrIIHygromycinpcr from lsm044-25,6205,6405,6605,6805,7005,7205,7405,760
    gactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgAjuIDraIIINmeAIIIHygromycinpcr from lsm044-25,7805,8005,8205,8405,8605,8805,9005,9205,940
    agacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgSacIIHygromycinpcr from lsm044-25,9605,9806,0006,0206,0406,0606,0806,1006,120
    tgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagcctScaIHygromycinpcr from lsm044-26,1406,1606,1806,2006,2206,2406,2606,2806,300
    atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccatagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtBsmINaeIPfoIPsiINgoMIVSV40 pApcr from lsm044-26,3206,3406,3606,3806,4006,4206,4406,460
    aactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgaBstZ17ISV40 pApcr from lsm044-26,4806,5006,5206,5406,5606,5806,6006,6206,640
    cacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtSapIBspQIpcr from lsm044-26,6606,6806,7006,7206,7406,7606,7806,8006,820
    gctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagacgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctPciIpcr from lsm044-2pUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccapUC oripcr from lsm044-27,0207,0407,0607,0807,1007,1207,1407,160
    cgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcgcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagAlwNIpUC oripcr from lsm044-27,1807,2007,2207,2407,2607,2807,3007,3207,340
    ttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcpUC oripcr from lsm044-27,3607,3807,4007,4207,4407,4607,4807,5007,520
    gatctcaactagagtt7,530
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7533 bp550060006500700050010001500HygromycinCMV promoter (with TetOs)CMVASV40 pAbla promoterAmp (bla)pcr from lsm044-2pUC oriEKo152.2 (DNA)p53_F (DNA)oLSM0118 (DNA)oLSM0126-temp (DNA)oLSM0113 (DNA)oLSM-pUC_ori_F (DNA)AhdINmeAIII,FspI,AclIPvuIScaI,BcgIAclI,XmnISspISgrDIAlwNIMfeI,Bpu10I,BsiWI,MluISpeINdeISnaBICspCINdeINmeAIIIDraIIIAjuISacIIHyg_F (DNA)ScaIPfoI,NgoMIV,NaeISV40PA_R (DNA),PsiI,BsmIBstZ17IAn-o17 U6_3MS2_Cre_seq (DNA)BspQI,SapIpUCori5F (DNA),oLSM-pUC_ori_R (DNA),PciIpUCoriR (DNA)AlwNI

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    7533

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