Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV-Ef1a-DIO\ChRmine-eYFP-Kv2.1-WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaagctttgcaaagatggataggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcgaaacgtttctacctatMluISbfIHindIIIL-ITREF-1a20406080100120140160
    aagttttaaacagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaattcaaaatttgtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccattAgeIMfeIEcoNIEF-1a180200220240260280300320340
    actgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggccctgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggEF-1a360380400420440460480500520
    ttgcgtgccttgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccaacgcacggaacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccaaccttcacccaccctctcaagctccggaacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggStuIStuIAflIIEF-1a540560580600620640660680700
    ttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacaagcgcggacagagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgctgcgaaaaaaagaccgttctatcagaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgPciIBfuAIBglIIBspMIEF-1a720740760780800820840860
    atgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttccctacaagccgctccgccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccggccggacgagaccacggaccggagcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggFseIPasIBstAPIEF-1a8809009209409609801,0001,0201,040
    ggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtccgggacgacgtccctcgagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgtttccttttcccggaaaggcaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcaSacITliIXhoIEcoNIPaeR7IEco53kIEF-1a1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttctgcagaaatccaacccccctccccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatccggtcgaaccgtgaactacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaEF-1a1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tccatttcaggtgtcgtgaggatccataacttcgtataggatactttatacgaagttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgccctattacacctcgttctcgtagcaaggtaaagtccacagcactcctaggtattgaagcatatcctatgaaatatgcttcaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcgggataatgtggagcaagagcatcgtAscIBamHIlox2722loxPEF-1aER\export1,4201,4401,4601,4801,5001,5201,5401,560
    gaagaatctggtggcttcagggaagtccgtggcacagctgatgaagctgtcaatgctggacatgctccgcatgtcgatgacgccctccgtgcgtgcgggcagaggggccacggggctgggcatgctgctcatctccagctcctctggaggcttgctctgcggggccatctccttgcttcttagaccaccgaagtcccttcaggcaccgtgtcgactacttcgacagttacgacctgtacgaggcgtacagctactgcgggaggcacgcacgcccgtctccccggtgccccgacccgtacgacgagtagaggtcgaggagacctccgaacgagacgccccggtagaggaacSphIKv2.11,5801,6001,6201,6401,6601,6801,7001,7201,740
    gtgttgaggatgggctgggactgcttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgacacaactcctacccgaccctgacgaacatgtcgagcaggtacggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacgagtcccgcctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctBsrGIEYFPKv2.11,7601,7801,8001,8201,8401,8601,8801,9001,920
    tgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaaacccccacaagacgaccatcaccagccgctcgacgtgcgacggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggaggaacttEYFP1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgcagctacgggaagtcgagctacgccaagtggtcccacagcgggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcEYFP2,1202,1402,1602,1802,2002,2202,2402,260
    aagcactgcaggccgtagccgaaggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatggttcgtgacgtccggcatcggcttccaccagtgctcccacccggtcccgtgcccgtcgaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctaccEYFP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gcaccaccccggtgaacagctcctcgcccttgctcaccacgttgatgtcgatctggtccagggggatgtactcgccctcgctggtgatcctgctcttggcggccgcagacagtctccgcaggcgctcgccattgctgctcagcctcatatccagctccagcttctggaactcatccgtggtggggccacttgtcgaggagcgggaacgagtggtgcaactacagctagaccaggtccccctacatgagcgggagcgaccactaggacgagaaccgccggcgtctgtcagaggcgtccgcgagcggtaacgacgagtcggagtataggtcgaggtcgaagaccttgagtagBsaBIChRmineTSEYFP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ctcgtcgtgtcccagctgctccagcagccgaaacagctcctcatcataggtctttctgaaccgggccagggcaaagccgtaacaggacttggccacgatgtcgcacacgcagtgggccacctctgtccagttatcgtcgatccagccgaagcctctagggcagatggcccacaccgagcagcacagggtcgacgaggtcgtcggctttgtcgaggagtagtatccagaaagacttggcccggtcccgtttcggcattgtcctgaaccggtgctacagcgtgtgcgtcacccggtggagacaggtcaatagcagctaggtcggcttcggagatcccgtctaccgggtgtggChRmine2,6402,6602,6802,7002,7202,7402,7602,7802,800
    aggggaaacagaatccacagcatggagaaggtaaacacggcgaacttcagcacgtgcaggctgtgctcggcgcctgtatcctgggccagctgggccagccggctatactgcttggccacgatgctcatcacgatagagtaggcgaagataaaccagaaacagccaaagccatacctcccctttgtcttaggtgtcgtacctcttccatttgtgccgcttgaagtcgtgcacgtccgacacgagccgcggacataggacccggtcgacccggtcggccgatatgacgaaccggtgctacgagtagtgctatctcatccgcttctatttggtctttgtcggtttcggtatggPmlISfiIBsaBIBstAPIChRmine2,8202,8402,8602,8802,9002,9202,9402,960
    aggcgtaggcgccattcctcagcctagggtcgccctctgcgtagaactcggccagcacgccagacatcaggatggcaaagatgatggcgctacaagacacgcgatagggggccctcagctgatacagcagatcgtacaccagcattgggcaggtcagcatgtagtcggcataccttccgcatccgcggtaaggagtcggatcccagcgggagacgcatcttgagccggtcgtgcggtctgtagtcctaccgtttctactaccgcgatgttctgtgcgctatcccccgggagtcgactatgtcgtctagcatgtggtcgtaacccgtccagtcgtacatcagccgtatggaAvrIIBfuAIBspMIChRmine2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gtaattgccgaagccgatgatgaaggggcccacatcgccgaagttgtggcccagtgtgtcggccacgatcttgccgaagtagctcagcatggcaaagaaattcacaaacagggccaggttctgctcctcctgatagccggcgtaaaaggaggttctcttgctagggccccgagatcattaacggcttcggctactacttccccgggtgtagcggcttcaacaccgggtcacacagccggtgctagaacggcttcatcgagtcgtaccgtttctttaagtgtttgtcccggtccaagacgaggaggactatcggccgcattttcctccaagagaacgatcccggggctctaChRmine3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtccaggactcatagcccaggtagaatgtggtgattgtcagcacgataaagcaagaccagtgggcgccgatggccacggagttcagcttggtatccagataccagccgtccattgtgcccacgtagaacatctggtcggtgcctggtgcgtgtgccatggtggctagcataacttcaggtcctgagtatcgggtccatcttacaccactaacagtcgtgctatttcgttctggtcacccgcggctaccggtgcctcaagtcgaaccataggtctatggtcggcaggtaacacgggtgcatcttgtagaccagccacggaccacgcacacggtaccaccgatcgtattgaaBmtINcoINheIXcmICspCIChRmine3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaatacttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgacClaIBspDIEcoRIEcoRVHindIIIWPREloxPlox27223,5203,5403,5603,5803,6003,6203,6403,660
    gtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgWPRE3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcWPRE3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggaccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctBbsIEarIZraIAatIIWPRE4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggAfeIClaITliIXhoIBglIIBspDICspCIPaeR7IhGH\polyAWPRE4,2204,2404,2604,2804,3004,3204,3404,360
    ttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggcBbsIhGH\polyA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    agttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggAloIBsaINsiISphIBsaXIhGH\polyA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcaacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtPmlIRsrIIBstEIIR-ITR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaSbfIf1\OriginR-ITR4,9204,9404,9604,9805,0005,0205,0405,060
    cctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagf1\Origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttaAloIPsiIBsaXIf1\Origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    attaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggtaattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctcc5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgcaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgZraIAatII5,6205,6405,6605,6805,7005,7205,7405,760
    tcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgcEarIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    agtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaaaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattPvuIScaIAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ctcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcagggagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtccAseIFspIAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    accacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagtggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtcBsaIAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    atcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagtagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaagcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttpUC\ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggcatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgpUC\ori7,0207,0407,0607,0807,1007,1207,1407,160
    tgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccpUC\ori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIpUC\ori7,3607,3807,4007,4207,4407,4607,4807,500
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    7509 bp550060006500700050010001500EYFPL-ITR,SbfI,MluI,HindIII,MfeI,AgeI,EcoNIEF-1a,StuI,AflII,StuI,BfuAI,BspMIBglII,PciI,FseI,PasIBstAPIEcoNI,Eco53kI,SacI,PaeR7I,TliI,XhoIlox2722,loxP,BamHI,AscIER\export,Kv2.1,SphIBsrGIZraI,AatIIEarIAmpR,ScaI,PvuIFspI,AseIBsaIpUC\ori,PciI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    7509