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AAV-KrasWT/sgKras/Cre
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    GCCATGTTTAAGGGTTCCTCTAGtgtacACGCGTtgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctCGGTACAAATTCCCAAGGAGATCacatgTGCGCAacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaMluIBGH polyA20406080100120140160
    ggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggaACGCGTgatatcagatctgttacgtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctTGCGCActatagtctagacaatgcatctattcatcgtaccgcccaattagtaattgatgttccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagBbsIMluIMscIBglIIBGH polyAITR180200220240260280300320340
    gctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccagctgagatctcctaggggcccatggctcgagcttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaKpnISalIXbaIBamHIAcc65IITR360380400420440460480500520
    tacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgFspI540560580600620640660680700
    aaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcggaatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaaNaeINgoMIVRep_Ori720740760780800820840860
    agggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgtcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgagatagagcAloIRep_Ori8809009209409609801,0001,0201,040
    ggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacaccccgataagaaaactaaatattccctaaaacggctaaagccagataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtggRep_Ori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgatgcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtacta1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcttattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagZraIAatIIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
    gcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgacgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactXmnIAmpR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccatactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtaAmpR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    aaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgtAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    atggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcgtaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgcBsaIFspINmeAIIIAmpR2,1202,1402,1602,1802,2002,2202,2402,260
    gtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagtattggtaactgtcagaccaagtttactcatatatactttagattgacatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcataaccattgacagtctggttcaaatgagtatatatgaaatctaactAmpR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtRep-origin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgcttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgRep-origin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    taatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgaRep-origin2,8202,8402,8602,8802,9002,9202,9402,960
    atgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtRep-origin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcaccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtRep-origin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacacgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtSapIBspQI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggcagctgcgcgctcgaacttcatgcctgccgaccttccccaggtcacgatccggacggcgggtgagttcacagaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgaacgtacggacgtccgtcgacgcgcgagcttgaagtacggacggctggaaggggtccagtgctaggcctgccgcccactcaagtgtBspEIEcoNIHindIII3,5203,5403,5603,5803,6003,6203,6403,660
    ttttarcagccggacgtgcaractccgctggtggtctaacgtcggttaggtcccttgaatcacgggacatatgttggtgttggaggtcctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagaaaatygtcggcctgcacgtytgaggcgaccaccagattgcagccaatccagggaacttagtgccctgtatacaaccacaacctccaggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcNdeIITR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cgcgcagagagggagtggccaactccatcactaggggttcctggaggggtggagtcgtgacgtaaagatctgatatcatcgatcgcgatgcattaattaagcggccgccatggCAGTTCTTTTGTATCCATTTGTCTCTTTATCTGCAATAATATATGTTCTACAAGGGAAAGGAgcgcgtctctccctcaccggttgaggtagtgatccccaaggacctccccacctcagcactgcatttctagactatagtagctagcgctacgtaattaattcgccggcggtaccGTCAAGAAAACATAGGTAAACAGAGAAATAGACGTTATTATATACAAGATGTTCCCTTTCCTBtgIClaIEagIMscINcoINotINruINsiIPacIBglIIBspDIKrasHR 2.1kbITR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GATAAAGTGTATATATTAATTTTATTTCAAAAATGTTTGGAAAAGCAGACTAGGAAACAACAGTGTTGTATTCAGGGCTGTTAACTGTATACAGTGCCAGCACTTAGTGCACCTGTTGGGAGTGCAGTGTATTATAAGCAAGGCAGAAGTCACAGAGGTTACTGAGAAACTCTTTCTATTTCACATATATAATTAAAATAAAGTTTTTACAAACCTTTTCGTCTGATCCTTTGTTGTCACAACATAAGTCCCGACAATTGACATATGTCACGGTCGTGAATCACGTGGACAACCCTCACGTCACATAATATTCGTTCCGTCTTCAGTGTCTCCAATGACTCTTTGAGAAAHpaIBstAPIBstZ17IKrasHR 2.1kb4,0404,0604,0804,1004,1204,1404,1604,1804,200
    TATGTTCTTTACATTTTTTTTCTTGCCAAAAATAGTCTTTATCTCTGAATTTCATTCAATTTTATTTCCTTGTACAGAGGTACTGTTTTTAGTACTCAAAGGATTATATGTTAAAATTGATCTGATCCTATACCAAATATTTAAACCCATTTCCCACAGATAGTTTATAGATTAAATACAAGAAATGTAAAAAAAAGAACGGTTTTTATCAGAAATAGAGACTTAAAGTAAGTTAAAATAAAGGAACATGTCTCCATGACAAAAATCATGAGTTTCCTAATATACAATTTTAACTAGACTAGGATATGGTTTATAAATTTGGGTAAAGGGTGTCTATCAAATATCTAATTKrasHR 2.1kb4,2204,2404,2604,2804,3004,3204,3404,360
    AATGCCATCAAATATTATCTTTATTTGCTTCATTGCCTAAATATGTCTTTCCCCAGCACAGTGCAGTTTTGACACCAGCTTCGGCTTCCTATTTTGTTGCCCTTTATTGCAGAACTGCTCTGATGGCTTTAAAACAGTTCTCTGCAGTTGTTGGCTCCAACACAGATGTTCTTAGTTACGGTAGTTTATAATAGAAATAAACGAAGTAACGGATTTATACAGAAAGGGGTCGTGTCACGTCAAAACTGTGGTCGAAGCCGAAGGATAAAACAACGGGAAATAACGTCTTGACGAGACTACCGAAATTTTGTCAAGAGACGTCAACAACCGAGGTTGTGTCTACAAGAATCKrasHR 2.1kb4,3804,4004,4204,4404,4604,4804,5004,5204,540
    GCTACCTAACTTCTAACTTTTAATATCCAGTCAACAAAGAATACCGCAAGGGTAGGTGTTGGGATAGCTGTCGACAAGCTCATGCGGGTGTGTCCACAGGGTATAGCGTACTATGCAGAATATTTGTACTGAGTGAAGTCATGATACATTCCTTTGAGAGCCATTAGCTGCTACACGATGGATTGAAGATTGAAAATTATAGGTCAGTTGTTTCTTATGGCGTTCCCATCCACAACCCTATCGACAGCTGTTCGAGTACGCCCACACAGGTGTCCCATATCGCATGATACGTCTTATAAACATGACTCACTTCAGTACTATGTAAGGAAACTCTCGGTAATCGACGATGTBarISalIKrasHR 2.1kb4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AAACAGTAATCTGGCTGTTTAGATCAACAAGCTAAATGATAGAAGATGAAAGTACTGGTTTCCATGTATTTTTATTAAGTGTTGATGAGAAAGTTGTAAGTGACTTACAGGTTACTCTGTACATCTGTAGTCACTGAATTCGGAATATCTTAGAGTTTTACACACctAGGTGAGTTTTGTCATTAGACCGACAAATCTAGTTGTTCGATTTACTATCTTCTACTTTCATGACCAAAGGTACATAAAAATAATTCACAACTACTCTTTCAACATTCACTGAATGTCCAATGAGACATGTAGACATCAGTGACTTAAGCCTTATAGAATCTCAAAATGTGTGgaTCCACTCAAleIAvrIIKrasHR 2.1kb4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTTAAAATATTGATAAAGTTTTTGATAATCTTGTGTGAGACATGTTCTAATTTAGTTGTATTTTATTATTTTTATTGTAAGGCCTGCTGAAAATGACTGAGTATAAACTaGTaGTcGTTGGAGCTGGTGGCGTAGGCAAGTCGGCACTGACGATACAGCTAATTCAGAATCACTTCAATTTTATAACTATTTCAAAAACTATTAGAACACACTCTGTACAAGATTAAATCAACATAAAATAATAAAAATAACATTCCGGACGACTTTTACTGACTCATATTTGAtCAtCAgCAACCTCGACCACCGCATCCGTTCAGCCGTGACTGCTATGTCGATTAAGTCTTAGTGAASpeIStuIM1T2E3Y4K5L6V7V8V9G10A11G12G13V14G15K16S17A18L19T20I21Q22L23I24Q25N26H27F284993-5104KrasHR 2.1kbexon2BarcodesgKras4,9204,9404,9604,9805,0005,0205,0405,060
    TGTGGATGAGTATGACCCTACGATAGAGGTAACGCTGCTCTACAGTCTGCGTaCGCTTGTAAAGGACGGCAGCCAGCCGCTTTGAAAAAGATATCATTTTTATATTTATTAGAAAATTATATTGAAAGTTATTTCAGTTATATGTGATGTCCTTTAGTTCCAAGGCTTTAAACTGACACCTACTCATACTGGGATGCTATCTCCATTGCGACGAGATGTCAGACGCAtGCGAACATTTCCTGCCGTCGGTCGGCGAAACTTTTTCTATAGTAAAAATATAAATAATCTTTTAATATAACTTTCAATAAAGTCAATATACACTACAGGAAATCAAGGTTCCGAAATTTGACBsiWIV29D30E31Y32D33P34T35I36E374993-5104KrasHR 2.1kbexon25,0805,1005,1205,1405,1605,1805,2005,2205,240
    GGTGTTAGGGAACCATAGGTGCAAGAAAGTCCACTTCTCATGAGAGCTCACCACAGAGAAAGAAAGTCCACTTCTCATGAGAGCTTACCACAGAGAGTAAGGTCCAAGATGCCATCCGTGCTTTGTATTTGCATTTTGATAAATAAATTTCATGATTTTGTGAGAGTTAAGAATACCACAATCCCTTGGTATCCACGTTCTTTCAGGTGAAGAGTACTCTCGAGTGGTGTCTCTTTCTTTCAGGTGAAGAGTACTCTCGAATGGTGTCTCTCATTCCAGGTTCTACGGTAGGCACGAAACATAAACGTAAAACTATTTATTTAAAGTACTAAAACACTCTCAATTCTTATKrasHR 2.1kb5,2605,2805,3005,3205,3405,3605,3805,4005,420
    TAGCTTTGCCTTTGATCTATTCCTGAAAGGTTTGTTGTTGTTTTATGGGTTTGTTGTTATTGTGTTGTTGTTTGGTGTCTTTTAGGCAGGGTCTTACTATGCAGCCCTGACCGGCCTAGAACTCAACTATGTAGACCAGACTAGCTCTGAATTCTCAGAGATCCATCTACCTGCCATCGAAACGGAAACTAGATAAGGACTTTCCAAACAACAACAAAATACCCAAACAACAATAACACAACAACAAACCACAGAAAATCCGTCCCAGAATGATACGTCGGGACTGGCCGGATCTTGAGTTGATACATCTGGTCTGATCGAGACTTAAGAGTCTCTAGGTAGATGGACGGKrasHR 2.1kb5,4405,4605,4805,5005,5205,5405,5605,5805,600
    TCTGCTGCCCAGATGCTGCGATTAAAGATGTACACCACCATGTACAGTGAAAGTTACTCTTTTGAAAACACTTTATTGTTATAGATTACTTGGAATGTTGATGTGATGAGAAAATTTGATTATACTAGGAAAATAGAATCACTTGATGGTTGAGCTTTTTTTTTAAGACTTATTTAGACGACGGGTCTACGACGCTAATTTCTACATGTGGTGGTACATGTCACTTTCAATGAGAAAACTTTTGTGAAATAACAATATCTAATGAACCTTACAACTACACTACTCTTTTAAACTAATATGATCCTTTTATCTTAGTGAACTACCAACTCGAAAAAAAAATTCTGAATAAAKrasHR 2.1kb5,6205,6405,6605,6805,7005,7205,7405,760
    ATTTTATGTAAGTGAGTACACTGCTTCTCTCTTCAGACACACCAGAAGAGGGCATCAGATCCCATTAAGATGGCTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAAGGCAGTCAGTGCTCTTTTTTTGTTTAAAGATTTATTTATTTTTATGAATACATAAAATACATTCACTCATGTGACGAAGAGAGAAGTCTGTGTGGTCTTCTCCCGTAGTCTAGGGTAATTCTACCGACACTCGGTGGTACACCAACGACCCTTAACTTGAGTCCTGGAGACCTTTCCGTCAGTCACGAGAAAAAAACAAATTTCTAAATAAATAAAAATACTTATGTXcmIBstXIPflMIBstAPIKrasHR 2.1kb5,7805,8005,8205,8405,8605,8805,9005,9205,940
    GTGTCACTGTCTTCATACACACAAGAGctcgaGCTGTGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGCACAGTGACAGAAGTATGTGTGTTCTCgagctCGACACTCCCGGATAAAGGGTACTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAACCTTAATTAAACTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACCCBbsITliIXhoIPspXIPaeR7IU6 promoterKrasHR 2.1kb5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACCAAACACCGACTGAGTATAAACTTGTGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCATAAAGCTAAAGAACCGAAATATATAGAACACCTTTCCTGGTTTGTGGCTGACTCATATTTGAACACCCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAANdeIU6 promoterChimeric RNA (& trRNA)gmKras6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTGTTTGGCGCGCCTATGGCTTAATTAACATGGCGGCCGCATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTAGTTGAACTTTTTCACCGTGGCTCAGCCACGAAAAAACAAACCGCGCGGATACCGAATTAATTGTACCGCCGGCGTAAGATGGCCCATCCCCTCCGCGAAAAGGGTTCCGTCAGACCTCGTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGACCGGAscIBsmIEagINotIPacIPGK promoterChimeric RNA (& trRNA)6,3206,3406,3606,3806,4006,4206,4406,460
    TCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCGTGTGTAAGGTGTAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGTGGAAGATGAGGAGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTGAgeISpeIBseRIPGK promoter6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCATCGTGGCGACTCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAAAGACCCGAGTCTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCCCCGCCCGCGGGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTBlpIBsmIStuIBspEIPGK promoter6,6606,6806,7006,7206,7406,7606,7806,8006,820
    CGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGACCCGGGATGCCCAAGAAGAAGAGGAAGGTGTCCAATTTACTGACCGTACACCAAAATTTGCCTGCATTACCGGTCGATGCAACGAGTGATGAGGTTCGCAAGAACCTGATGGACATGTTCAGGCGAAGTTTTCGCGTGCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCTGGGCCCTACGGGTTCTTCTTCTCCTTCCACAGGTTAAATGACTGGCATGTGGTTTTAAACGGACGTAATGGCCAGCTACGTTGCTCACTACTCCAAGCGTTCTTGGACTACCTGTACAAGTCAgeIKflIBseRIMP2KK4KR6KV8SN10LL12TV14HQ16NL18PA20LP22VD24AT26SD28EV30RK32NL34MD36MF38R6885-7936PGK promoterCre6,8406,8606,8806,9006,9206,9406,9606,9807,000
    GGATCGCCAGGCGTTTTCTGAGCATACCTGGAAAATGCTTCTGTCCGTTTGCCGGTCGTGGGCGGCATGGTGCAAGTTGAATAACCGGAAATGGTTTCCCGCAGAACCTGAAGATGTTCGCGATTATCTTCTATATCTTCAGGCGCGCGGTCTGGCAGTAAAAACTATCCAGCAACCTAGCGGTCCGCAAAAGACTCGTATGGACCTTTTACGAAGACAGGCAAACGGCCAGCACCCGCCGTACCACGTTCAACTTATTGGCCTTTACCAAAGGGCGTCTTGGACTTCTACAAGCGCTAATAGAAGATATAGAAGTCCGCGCGCCAGACCGTCATTTTTGATAGGTCGTTNruIXmnID40RQ42AF44SE46HT48WK50ML52LS54VC56RS58WA60AW62CK64LN66NR68KW70FP72AE74PE76DV78RD80YL82LY84LQ86AR88GL90AV92KT94IQ96Q6885-7936Cre7,0207,0407,0607,0807,1007,1207,1407,160
    CATTTGGGCCAGCTAAACATGCTTCATCGTCGGTCCGGGCTGCCACGACCAAGTGACAGCAATGCTGTTTCACTGGTTATGCGGCGGATCCGAAAAGAAAACGTTGATGCCGGTGAACGTGCAAAACAGGCTCTAGCGTTCGAACGCACTGATTTCGACCAGGTTCGTTCACTCAGTAAACCCGGTCGATTTGTACGAAGTAGCAGCCAGGCCCGACGGTGCTGGTTCACTGTCGTTACGACAAAGTGACCAATACGCCGCCTAGGCTTTTCTTTTGCAACTACGGCCACTTGCACGTTTTGTCCGAGATCGCAAGCTTGCGTGACTAAAGCTGGTCCAAGCAAGTGAGTBamHIBstBIRsrIISexAIH98LG100QL102NM104LH106RR108SG110LP112RP114SD116SN118AV120SL122VM124RR126IR128KE130NV132DA134GE136RA138KQ140AL142AF144ER146TD148FD150QV152RS154L6885-7936Cre7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TGGAAAATAGCGATCGCTGCCAGGATATACGTAATCTGGCATTTCTGGGGATTGCTTATAACACCCTGTTACGTATAGCCGAAATTGCCAGGATCAGGGTTAAAGATATCTCACGTACTGACGGTGGGAGAATGTTAATCCATATTGGCAGAACGAAAACGCTGGTTAGCACCGCACCTTTTATCGCTAGCGACGGTCCTATATGCATTAGACCGTAAAGACCCCTAACGAATATTGTGGGACAATGCATATCGGCTTTAACGGTCCTAGTCCCAATTTCTATAGAGTGCATGACTGCCACCCTCTTACAATTAGGTATAACCGTCTTGCTTTTGCGACCAATCGTGGCGAsiSIPaqCIM156EN158SD160RC162QD164IR166NL168AF170LG172IA174YN176TL178LR180IA182EI184AR186IR188VK190DI192SR194TD196GG198RM200LI202HI204GR206TK208TL210VS212TA2146885-7936Cre7,3607,3807,4007,4207,4407,4607,4807,5007,520
    AGGTGTAGAGAAGGCACTTAGCCTGGGGGTAACTAAACTGGTCGAGCGATGGATTTCCGTCTCTGGTGTAGCTGATGATCCGAATAACTACCTGTTTTGCCGGGTCAGAAAAAATGGTGTTGCCGCGCCATCTGCCACCAGCCAGCTATCAACTCGCGCCCTGGAAGGGATTTTTTCCACATCTCTTCCGTGAATCGGACCCCCATTGATTTGACCAGCTCGCTACCTAAAGGCAGAGACCACATCGACTACTAGGCTTATTGATGGACAAAACGGCCCAGTCTTTTTTACCACAACGGCGCGGTAGACGGTGGTCGGTCGATAGTTGAGCGCGGGACCTTCCCTAAAAAGV216EK218AL220SL222GV224TK226LV228ER230WI232SV234SG236VA238DD240PN242NY244LF246CR248VR250KN252GV254AA256PS258AT260SQ262LS264TR266AL268EG270IF2726885-7936Cre7,5407,5607,5807,6007,6207,6407,6607,6807,700
    GAAGCAACTCATCGATTGATTTACGGCGCTAAGGATGACTCTGGTCAGAGATACCTGGCCTGGTCTGGACACAGTGCCCGTGTCGGAGCCGCGCGAGATATGGCCCGCGCTGGAGTTTCAATACCGGAGATCATGCAAGCTGGTGGCTGGACCAATGTAAATATTGTCATGAACTCTTCGTTGAGTAGCTAACTAAATGCCGCGATTCCTACTGAGACCAGTCTCTATGGACCGGACCAGACCTGTGTCACGGGCACAGCCTCGGCGCGCTCTATACCGGGCGCGACCTCAAAGTTATGGCCTCTAGTACGTTCGACCACCGACCTGGTTACATTTATAACAGTACTTGAClaIBspDIPshAIEA274TH276RL278IY280GA282KD284DS286GQ288RY290LA292WS294GH296SA298RV300GA302AR304DM306AR308AG310VS312IP314EI316MQ318AG320GW322TN324VN326IV328MN3306885-7936Cre7,7207,7407,7607,7807,8007,8207,8407,860
    ATATCCGTAACCTGGATAGTGAAACAGGGGCAATGGTGCGCCTGCTGGAAGATGGCGATTATATAGGCATTGGACCTATCACTTTGTCCCCGTTACCACGCGGACGACCTTCTACCGCTAATYI332RN334LD336SE338TG340AM342VR344LL346ED348GD3506885-7936Cre7,8807,9007,920
    • Plasmid
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    • Linear Map
    Editing is disabled because sequence edits have been locked.
    7936 bp5500600065007000750050010001500KrasHR 2.1kbAmpRCre6885-7936

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    7936