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pC0045 EF1a-PguCas13b-NES-HIV
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaacagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttSgrDI20406080100120140160
    tctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccagacgaatcccaatccgcaaaacgcgacgaagcgctacatgcccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggMluINruISpeICMV enhancer180200220240260280300320340
    aacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcNdeICMV enhancer360380400420440460480500520
    cctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaaCspCISnaBICMV enhancer540560580600620640660680700
    gttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtcgcgcaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatccctt5'LTR720740760780800820840860880
    cccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagag5'LTR9009209409609801,0001,0201,0401,060
    gacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtcccccNruIBssHII1,0801,1001,1201,1401,1601,1801,2001,2201,240
    aaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaacctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgg1,2601,2801,3001,3201,3401,3601,3801,4001,420
    ctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtagagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatttcatcatNotISapIBspQI1,4401,4601,4801,5001,5201,5401,5601,5801,600
    aaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagaccatatcRRE1,6201,6401,6601,6801,7001,7201,7401,7601,780
    tgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggtgacgacaAleIBbvCIRRE1,8001,8201,8401,8601,8801,9001,9201,940
    gccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctatttacccgt1,9601,9802,0002,0202,0402,0602,0802,1002,120
    agtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggactcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggttggggctcccctgKflI2,1402,1602,1802,2002,2202,2402,2602,2802,300
    ccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagccgtgacgcacgcggttaagacgtctgtttaccgtcataagtaggtgttaaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatc2,3202,3402,3602,3802,4002,4202,4402,4602,480
    acataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttggttaattaatgcaaagatggataaagttttaaacagagaggaatctttgcagctaatggaccttctaggtcttgaaagtgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctctaggtcaaaccaattaattacgtttctacctatttcaaaatttgtctctccttagaaacgtcgattacctggaagatccagaactttcPacIEF-1a2,5002,5202,5402,5602,5802,6002,6202,6402,660
    gagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccctcacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggAgeIEF-1a2,6802,7002,7202,7402,7602,7802,8002,8202,840
    gtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggaacgcacggaacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccEF-1a2,8602,8802,9002,9202,9402,9602,9803,0003,020
    ttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcaaccttcacccaccctctcaagctccggaacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggaagcgcggacagagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgEF-1a3,0403,0603,0803,1003,1203,1403,1603,1803,200
    gacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggcctgcgaaaaaaagaccgttctatcagaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgtacaagccgctccgccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccgApaIPciIBfuAIBspMIPspOMIEF-1a3,2203,2403,2603,2803,3003,3203,3403,3603,380
    cggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacgccggacgagaccacggaccggagcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggccgggacgacgtccctcgagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgFseIPasIEF-1a3,4003,4203,4403,4603,4803,5003,5203,5403,560
    aaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttagtttccttttcccggaaaggcaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcagcagaaatccaacccccctccccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatcTliIXhoIBbvCIPaeR7IEF-1a3,5803,6003,6203,6403,6603,6803,7003,720
    gccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgacGTACGGCCACCatgacagagcagagcgagaggccctacaacggcacctactacacccggtcgaaccgtgaactacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaaggtaaagtccacagcactgCATGCCGGTGGtactgtctcgtctcgctctccgggatgttgccgtggatgatgtggBsiWIMT2EQ4SE6RP8YN10GT12YY14T3872-7396EF-1aPguCas13b3,7403,7603,7803,8003,8203,8403,8603,8803,900
    ctggaagataagcacttctgggccgcctttctgaacctggccagacacaacgcctacatcaccctgacacacatcgaccggcagctggcctacagcaaggccgacatcaccaacgaccaggacgtgctgagcttcaaggccctgtggaagaacttcgacaacgacctggaaagaaagagaccttctattcgtgaagacccggcggaaagacttggaccggtctgtgttgcggatgtagtgggactgtgtgtagctggccgtcgaccggatgtcgttccggctgtagtggttgctggtcctgcacgactcgaagttccgggacaccttcttgaagctgttgctggacctttctttctBlpIL16ED18KH20FW22AA24FL26NL28AR30HN32AY34IT36LT38HI40DR42QL44AY46SK48AD50IT52ND54QD56VL58SF60KA62LW64KN66FD68ND70LE72RK743872-7396PguCas13b3,9203,9403,9603,9804,0004,0204,0404,0604,080
    gccggctgcggagcctgatcctgaagcacttcagctttctggaaggcgccgcttacggcaagaagctgttcgagtctaagagcagcggcaacaagagcagcaagaacaaagagctgaccaagaaagagaaagaggaactgcaggccaacgctctgagcctggacaacctgaagtctatcggccgacgcctcggactaggacttcgtgaagtcgaaagaccttccgcggcgaatgccgttcttcgacaagctcagattctcgtcgccgttgttctcgtcgttcttgtttctcgactggttctttctctttctccttgacgtccggttgcgagactcggacctgttggacttcagataSR76LR78SL80IL82KH84FS86FL88EG90AA92YG94KK96LF98ES100KS102SG104NK106SS108KN110KE112LT114KK116EK118EE120LQ122AN124AL126SL128DN130LK132SI1343872-7396PguCas13b4,1004,1204,1404,1604,1804,2004,2204,2404,260
    cctgttcgacttcctgcagaagctgaaggacttccggaactactacagccactaccggcacagcggaagcagcgagctgcctctgttcgacggcaacatgctgcagcggctgtacaacgtgttcgacgtgtccgtgcagcgcgtgaagatcgaccacgagcacaacgatgaggtggacggacaagctgaaggacgtcttcgacttcctgaaggccttgatgatgtcggtgatggccgtgtcgccttcgtcgctcgacggagacaagctgccgttgtacgacgtcgccgacatgttgcacaagctgcacaggcacgtcgcgcacttctagctggtgctcgtgttgctactccacctgPsrILF136DF138LQ140KL142KD144FR146NY148YS150HY152RH154SG156SS158EL160PL162FD164GN166ML168QR170LY172NV174FD176VS178VQ180RV182KI184DH186EH188ND190EV192D3872-7396PguCas13b4,2804,3004,3204,3404,3604,3804,4004,4204,440
    cctcactaccacttcaaccacctcgtgcggaagggcaagaaggacagatacggccacaacgacaaccccagcttcaagcaccacttcgtggacggcgaaggcatggttacagaagccggcctgctgttcttcgtgtccctgttcctggaaaagcgggacgccatctggatgcagaagaggagtgatggtgaagttggtggagcacgccttcccgttcttcctgtctatgccggtgttgctgttggggtcgaagttcgtggtgaagcacctgccgcttccgtaccaatgtcttcggccggacgacaagaagcacagggacaaggaccttttcgccctgcggtagacctacgtcttctBarIP194HY196HF198NH200LV202RK204GK206KD208RY210GH212ND214NP216SF218KH220HF222VD224GE226GM228VT230EA232GL234LF236FV238SL240FL242EK244RD246AI248WM250QK2523872-7396PguCas13b4,4604,4804,5004,5204,5404,5604,5804,6004,620
    agatccggggcttcaaaggcggcaccgagacataccagcagatgaccaacgaggtgttctgccggtccagaatcagcctgcctaagctgaagctggaaagcctgcggatggacgactggatgctgctggacatgctgaacgaactcgtgcggtgccccaagcctctgtacgacagacttctaggccccgaagtttccgccgtggctctgtatggtcgtctactggttgctccacaagacggccaggtcttagtcggacggattcgacttcgacctttcggacgcctacctgctgacctacgacgacctgtacgacttgcttgagcacgccacggggttcggagacatgctgtctgaAjuIKI254RG256FK258GG260TE262TY264QQ266MT268NE270VF272CR274SR276IS278LP280KL282KL284ES286LR288MD290DW292ML294LD296ML298NE300LV302RC304PK306PL308YD310RL3123872-7396PguCas13b4,6404,6604,6804,7004,7204,7404,7604,7804,800
    gagagaggacgaccgggcctgctttagagtgcccgtggatatcctgcctgacgaggacgatacagatggcggcggagaggaccccttcaagaataccctcgtccggcaccaggacagattcccttacttcgccctgcggtacttcgacctgaagaaggtgttcaccagcctgcggttcctctctcctgctggcccggacgaaatctcacgggcacctataggacggactgctcctgctatgtctaccgccgcctctcctggggaagttcttatgggagcaggccgtggtcctgtctaagggaatgaagcgggacgccatgaagctggacttcttccacaagtggtcggacgccaagRE314DD316RA318CF320RV322PV324DI326LP328DE330DD332TD334GG336GE338DP340FK342NT344LV346RH348QD350RF352PY354FA356LR358YF360DL362KK364VF366TS368LR370F3872-7396PguCas13b4,8204,8404,8604,8804,9004,9204,9404,9604,980
    cacatcgatctgggcacctaccactttgccatctacaagaagatgatcggcgagcagcccgaggacagacacctgaccagaaacctgtacggcttcggccggatccaggacttcgccgaagaacacagacccgaggaatggaagcggctcgtcagagatctggactacttcgagacaggtgtagctagacccgtggatggtgaaacggtagatgttcttctactagccgctcgtcgggctcctgtctgtggactggtctttggacatgccgaagccggcctaggtcctgaagcggcttcttgtgtctgggctccttaccttcgccgagcagtctctagacctgatgaagctctgtcBamHIH372ID374LG376TY378HF380AI382YK384KM386IG388EQ390PE392DR394HL396TR398NL400YG402FG404RI406QD408FA410EE412HR414PE416EW418KR420LV422RD424LD426YF428ET4303872-7396PguCas13b5,0005,0205,0405,0605,0805,1005,1205,1405,160
    gcgacaagccctacatcagccagacaagcccacactaccacatcgagaagggaaagatcggcctgagattcatgcccgagggccagcatctttggccctctccagaagtgggcaccaccagaaccggcagatctaagtacgcccaggacaagagactgaccgccgaggcctttctgtccgctgttcgggatgtagtcggtctgttcgggtgtgatggtgtagctcttccctttctagccggactctaagtacgggctcccggtcgtagaaaccgggagaggtcttcacccgtggtggtcttggccgtctagattcatgcgggtcctgttctctgactggcggctccggaaagacagGD432KP434YI436SQ438TS440PH442YH444IE446KG448KI450GL452RF454MP456EG458QH460LW462PS464PE466VG468TT470RT472GR474SK476YA478QD480KR482LT484AE486AF488LS4903872-7396PguCas13b5,1805,2005,2205,2405,2605,2805,3005,3205,340
    tgtgcacgagctgatgcctatgatgttctactacttcctgctgcgcgagaagtacagcgaggaagtgtctgccgagagagtgcagggcagaatcaagcgcgtgatcgaggatgtgtacgccgtgtacgatgcctttgccagggacgagatcaacacccgggatgagctggatgcctgcacacgtgctcgactacggatactacaagatgatgaaggacgacgcgctcttcatgtcgctccttcacagacggctctctcacgtcccgtcttagttcgcgcactagctcctacacatgcggcacatgctacggaaacggtccctgctctagttgtgggccctactcgacctacggacgVH492EL494MP496MM498FY500YF502LL504RE506KY508SE510EV512SA514ER516VQ518GR520IK522RV524IE526DV528YA530VY532DA534FA536RD538EI540NT542RD544EL546DA548C3872-7396PguCas13b5,3605,3805,4005,4205,4405,4605,4805,500
    ctggccgataagggcatcagaagaggccatctgcctcgccagatgatcgccatcctgagccaagagcacaaggacatggaagagaagattcggaagaaactgcaagagatgatggccgacaccgaccaccggctggatatgctggatagacagaccgaccggaagatcagaatcggccgaccggctattcccgtagtcttctccggtagacggagcggtctactagcggtaggactcggttctcgtgttcctgtaccttctcttctaagccttctttgacgttctctactaccggctgtggctggtggccgacctatacgacctatctgtctggctggccttctagtcttagccggBsaBIBsaBIL550AD552KG554IR556RG558HL560PR562QM564IA566IL568SQ570EH572KD574ME576EK578IR580KK582LQ584EM586MA588DT590DH592RL594DM596LD598RQ600TD602RK604IR606IG6083872-7396PguCas13b5,5205,5405,5605,5805,6005,6205,6405,6605,680
    ggaagaatgccggactgcctaagagcggagtgatcgccgattggctcgtgcgggacatgatgagatttcagcccgtggccaaggacgccagcggaaagcctctgaacaactccaaggccaacagcaccgagtaccggatgctgcagagagccctggctctgtttggaggcgagaaagaccttcttacggcctgacggattctcgcctcactagcggctaaccgagcacgccctgtactactctaaagtcgggcaccggttcctgcggtcgcctttcggagacttgttgaggttccggttgtcgtggctcatggcctacgacgtctctcgggaccgagacaaacctccgctctttctRK610NA612GL614PK616SG618VI620AD622WL624VR626DM628MR630FQ632PV634AK636DA638SG640KP642LN644NS646KA648NS650TE652YR654ML656QR658AL660AL662FG664GE666KE6683872-7396PguCas13b5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gaggctgaccccatacttccggcagatgaatctgaccggcggaaacaaccctcatccttttctgcacgaaactcgctgggagagccacaccaacatcctgtccttttaccggtcctacctgagagcccgcaaggccttcctcgaaaggatcggcagatccgacagagtggaaaaccggctccgactggggtatgaaggccgtctacttagactggccgcctttgttgggagtaggaaaagacgtgctttgagcgaccctctcggtgtggttgtaggacaggaaaatggccaggatggactctcgggcgttccggaaggagctttcctagccgtctaggctgtctcaccttttggccAgeIRL670TP672YF674RQ676MN678LT680GG682NN684PH686PF688LH690ET692RW694ES696HT698NI700LS702FY704RS706YL708RA710RK712AF714LE716RI718GR720SD722RV724EN726R3872-7396PguCas13b5,8805,9005,9205,9405,9605,9806,0006,0206,040
    ccatttctgctgctgaaagagcccaagaccgacagacagacactggtggctggatggaagggcgagttccatctgccaagaggcatcttcacagaggccgtgcgggattgcctgatcgagatgggacacgatgaagtggccagctacaaagaagtgggattcatggccaaggccgtgcggtaaagacgacgactttctcgggttctggctgtctgtctgtgaccaccgacctaccttcccgctcaaggtagacggttctccgtagaagtgtctccggcacgccctaacggactagctctaccctgtgctacttcaccggtcgatgtttcttcaccctaagtaccggttccggcacgP728FL730LL732KE734PK736TD738RQ740TL742VA744GW746KG748EF750HL752PR754GI756FT758EA760VR762DC764LI766EM768GH770DE772VA774SY776KE778VG780FM782AK784AV7863872-7396PguCas13b6,0606,0806,1006,1206,1406,1606,1806,2006,220
    cactgtactttgagagagcctgcgaggacagggtgcagcccttttacgacagccccttcaacgtgggcaacagcctgaagcctaagaagggcagattcctgagcaaagaggaacgggccgaagagtgggagcggggcaaagagagattccgggatctcgaagcctggtcctatagcgcgtgacatgaaactctctcggacgctcctgtcccacgtcgggaaaatgctgtcggggaagttgcacccgttgtcggacttcggattcttcccgtctaaggactcgtttctccttgcccggcttctcaccctcgccccgtttctctctaaggccctagagcttcggaccaggatatcgcgPL788YF790ER792AC794ED796RV798QP800FY802DS804PF806NV808GN810SL812KP814KK816GR818FL820SK822EE824RA826EE828WE830RG832KE834RF836RD838LE840AW842SY844SA8463872-7396PguCas13b6,2406,2606,2806,3006,3206,3406,3606,3806,400
    cgccagaagaatcgaggacgcctttgccggcatcgagtatgctagccccggaaacaagaagaaaatcgagcagctcctgcgggacctgagcctgtgggaagcctttgagagcaagctgaaagtgcgggccgaccggatcaatctggccaagctgaagaaagaaatcctggaagcccaagcggtcttcttagctcctgcggaaacggccgtagctcatacgatcggggcctttgttcttcttttagctcgtcgaggacgccctggactcggacacccttcggaaactctcgttcgactttcacgcccggctggcctagttagaccggttcgacttctttctttaggaccttcgggttBmtINheIAR848RI850ED852AF854AG856IE858YA860SP862GN864KK866KI868EQ870LL872RD874LS876LW878EA880FE882SK884LK886VR888AD890RI892NL894AK896LK898KE900IL902EA904Q3872-7396PguCas13b6,4206,4406,4606,4806,5006,5206,5406,5606,580
    gaacacccctaccacgacttcaagagctggcagaagttcgagcgcgagctgcggctggtcaagaaccaggatatcatcacctggatgatgtgccgcgacctgatggaagaaaacaaggtggaaggcctggacaccggcacactgtacctgaaggacatcagacccaacgtgcaagagccttgtggggatggtgctgaagttctcgaccgtcttcaagctcgcgctcgacgccgaccagttcttggtcctatagtagtggacctactacacggcgctggactaccttcttttgttccaccttccggacctgtggccgtgtgacatggacttcctgtagtctgggttgcacgttctcgE906HP908YH910DF912KS914WQ916KF918ER920EL922RL924VK926NQ928DI930IT932WM934MC936RD938LM940EE942NK944VE946GL948DT950GT952LY954LK956DI958RP960NV962QE9643872-7396PguCas13b6,6006,6206,6406,6606,6806,7006,7206,7406,760
    agggcagcctgaacgtgctgaacagagtgaagcccatgagactgcccgtggtggtgtacagagccgatagcagaggccacgtgcacaaagaagaagcccctctggccaccgtgtacatcgaggaaagagacaccaagctgctgaagcagggcaacttcaagtccttcgtgaaggacagtcccgtcggacttgcacgacttgtctcacttcgggtactctgacgggcaccaccacatgtctcggctatcgtctccggtgcacgtgtttcttcttcggggagaccggtggcacatgtagctcctttctctgtggttcgacgacttcgtcccgttgaagttcaggaagcacttcctgtcQG966SL968NV970LN972RV974KP976MR978LP980VV982VY984RA986DS988RG990HV992HK994EE996AP998LA1000TV1002YI1004EE1006RD1008TK1010LL1012KQ1014GN1016FK1018SF1020VK1022DR10243872-7396PguCas13b6,7806,8006,8206,8406,8606,8806,9006,9206,940
    gcggctgaacggcctgttctccttcgttgatactggcggactggccatggaacagtaccccatctccaagctgagagtggaatacgagctggccaagtaccagaccgccagagtgtgtgtgttcgagctgaccctgagactggaagagtccctgctgacccggtatcctcatctgccccgccgacttgccggacaagaggaagcaactatgaccgcctgaccggtaccttgtcatggggtagaggttcgactctcaccttatgctcgaccggttcatggtctggcggtctcacacacacaagctcgactgggactctgaccttctcagggacgactgggccataggagtagacgggXcmIRL1026NG1028LF1030SF1032VD1034TG1036GL1038AM1040EQ1042YP1044IS1046KL1048RV1050EY1052EL1054AK1056YQ1058TA1060RV1062CV1064FE1066LT1068LR1070LE1072ES1074LL1076TR1078YP1080HL1082P3872-7396PguCas13b6,9606,9807,0007,0207,0407,0607,0807,1007,120
    gacgagagcttcagagagatgctggaatcttggagcgaccctctgctggccaaatggcctgaactgcacggaaaagtgcggctgctgatcgccgtgcggaatgcctttagccacaatcagtaccctatgtacgacgaggccgtgttcagctccatccggaagtacgatcctagcagccctgctctcgaagtctctctacgaccttagaacctcgctgggagacgaccggtttaccggacttgacgtgccttttcacgccgacgactagcggcacgccttacggaaatcggtgttagtcatgggatacatgctgctccggcacaagtcgaggtaggccttcatgctaggatcgtcggD1084ES1086FR1088EM1090LE1092SW1094SD1096PL1098LA1100KW1102PE1104LH1106GK1108VR1110LL1112IA1114VR1116NA1118FS1120HN1122QY1124PM1126YD1128EA1130VF1132SS1134IR1136KY1138DP1140SS11423872-7396PguCas13b7,1407,1607,1807,2007,2207,2407,2607,280
    ccgacgccatcgaagagagaatgggcctgaatatcgcccaccgcctgtctgaggaagtgaagcaggccaaagaaaccgtggaacggatcattcaggccggatccCTTCAACTGCCTCCACTTGAAAGACTGACACTGGGTAGTTCCtacccatacgatgttccagattacgcttatccggctgcggtagcttctctcttacccggacttatagcgggtggcggacagactccttcacttcgtccggtttctttggcaccttgcctagtaagtccggcctaggGAAGTTGACGGAGGTGAACTTTCTGACTGTGACCCATCAAGGatgggtatgctacaaggtctaatgcgaataggXcmIBamHIG1S2L3Q4L5P6P7L8E9R10L11T12L13G14S15S16Y17P18Y19D20V21P22D23Y24A25Y26P277397-7528PD1144AI1146EE1148RM1150GL1152NI1154AH1156RL1158SE1160EV1162KQ1164AK1166ET1168VE1170RI1172IQ1174A3872-7396PguCas13bHIV NESHA tag 3x7,3007,3207,3407,3607,3807,4007,4207,4407,460
    ctacgacgtgcctgattatgcatacccatatgatgtccccgactatgcctaaGaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatgatgctgcacggactaatacgtatgggtatactacaggggctgatacggattCttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacataNdeIEcoRIY28D29V30P31D32Y33A34Y35P36Y37D38V39P40D41Y42A43*447397-7528WPREHA tag 3x7,4807,5007,5207,5407,5607,5807,6007,6207,640
    catgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctWPRE7,6607,6807,7007,7207,7407,7607,7807,8007,820
    ctttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaaWPRE7,8407,8607,8807,9007,9207,9407,9607,9808,000
    ctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacctcgagacctagaaaaacatggagcaatcacagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggcagctggagctctggatctttttgtacctcgttagtgtTliIXhoIPaeR7IWPRE8,0208,0408,0608,0808,1008,1208,1408,1608,180
    agtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactccctcatcgttatgtcgtcgatggttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttcccccctgaccttcccgattaagtgagggKpnIAcc65IBsu36I8,2008,2208,2408,2608,2808,3008,3208,3408,360
    aacgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgttgcttctgttctataggaactagacacctagatggtgtgtgttccgatgaagggactaaccgtcttgatgtgtggtcccggtccctagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcgttctcttccatcttcttcggttacttcctctcttgtgggc8,3808,4008,4208,4408,4608,4808,5008,5208,540
    cttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacgaacaatgtgggacactcggacgtaccctacctactgggcctctctcttcataatctcacctccaaactgtcggcggatcgtaaagtagtgtaccgggctctcgacgtaggcctgacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttg3' LTR8,5608,5808,6008,6208,6408,6608,6808,7008,720
    ccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccaggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcccgggcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtApaIBclIPmeIPspOMI3' LTR8,7408,7608,7808,8008,8208,8408,8608,8808,900
    tctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtac8,9208,9408,9608,9809,0009,0209,0409,060
    ctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcgacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaagF1 ori9,0809,1009,1209,1409,1609,1809,2009,2209,240
    ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcDrdIF1 ori9,2609,2809,3009,3209,3409,3609,3809,4009,420
    ttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaaaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttAloIPsiIF1 ori9,4409,4609,4809,5009,5209,5409,5609,5809,600
    gtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgccccaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggSexAI9,6209,6409,6609,6809,7009,7209,7409,7609,780
    agttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcacaBclIAvrII9,8009,8209,8409,8609,8809,9009,9209,9409,960
    tgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaaccatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcactgttaattagtagccgtatcatatagccgtatcatattatgctgttccactccttgatttggtaccggttcaactggtcacggcaaggccacgagtggcgcgcgctgcagcggcctcgccagctcaagacctggctggccgagcccaagagggccctgaagcacctcctgctgaagMauBIBssHII9,98010,00010,02010,04010,06010,08010,10010,12010,140
    gccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcgcggccacaccaggccctgctgcactgggacaagtagtcgcgccaggtcctggtccaccacggcctgttgtgggaccggacccacacccacgcgccggacctgctcgacatgcggctcaccagcctccagcacaggtgcttgaaggccctgcggaggcccggccggtactggctctagcFseISexAISgrAI10,16010,18010,20010,22010,24010,26010,28010,30010,320
    gcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggactgacacgtgctacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcgctcgtcggcacccccgccctcaagcgggacgcgctgggccggccgttgacgcacgtgaagcaccggctcctcgtcctgactgtgcacgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgc10,34010,36010,38010,40010,42010,44010,46010,48010,500
    cggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaccgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggPsiIBstZ17I10,52010,54010,56010,58010,60010,62010,64010,66010,680
    tctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagcccttlac promoterLacOM13-rev10,70010,72010,74010,76010,78010,80010,82010,840
    acctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataatggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattSapIBspQI10,86010,88010,90010,92010,94010,96010,98011,00011,020
    cgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggagcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggacctDrdIPciIColE1 origin11,04011,06011,08011,10011,12011,14011,16011,18011,200
    agctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgcttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgaColE1 origin11,22011,24011,26011,28011,30011,32011,34011,36011,380
    gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgccgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgColE1 origin11,40011,42011,44011,46011,48011,50011,52011,54011,560
    tctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaacagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgColE1 origin11,58011,60011,62011,64011,66011,68011,70011,72011,740
    tcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccaagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtBspHIAmpRColE1 origin11,76011,78011,80011,82011,84011,86011,88011,90011,920
    tagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaAhdIAmpR11,94011,96011,98012,00012,02012,04012,06012,08012,100
    ccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgtttFspIAmpR12,12012,14012,16012,18012,20012,22012,24012,26012,280
    aaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgactttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctgPvuIScaIAmpR12,30012,32012,34012,36012,38012,40012,42012,44012,460
    cgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttacgctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgAmpR12,48012,50012,52012,54012,56012,58012,60012,620
    tttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaaaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttSspIBspHI12,64012,66012,68012,70012,72012,74012,76012,78012,800
    caaataggggttccgcgcacatttccccgaaaagtgccacctgacgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactg12,82012,84012,860
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12861 bp9000100001100012000100020003000EF-1aCMV enhancer,SgrDI,NruI,MluI,SpeI,NdeI,SnaBI,CspCI5'LTR,BssHII,NruIBspQI,SapI,NotIRRE,BbvCIAleIKflIPacIAgeIBfuAI,BspMISexAIAvrII,BclI,BssHII,MauBISgrAI,SexAI,FseIPsiIM13-rev,LacO,lac promoter,BstZ17IBspQI,SapI,PciI,DrdIColE1 originBspHI,AhdIAmpR,FspI,PvuI,ScaI,SspI,BspHI

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