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pC0045 EF1a-PguCas13b-NES-HIV
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaacagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttSgrDI20406080100120140160
    gaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgacttagacgaatcccaatccgcaaaacgcgacgaagcgctacatgcccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactMluINruISpeICMV enhancer180200220240260280300320340
    ccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatNdeICMV enhancer360380400420440460480500520
    aatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcaCspCISnaBICMV enhancer540560580600620640660680700
    caatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctcgttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtcgcgcaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagag5'LTR720740760780800820840860
    tggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccct5'LTR8809009209409609801,0001,0201,040
    aaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaattggtctcctcgagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttNruIBssHII1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagattaagccaattccggtccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatct1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtaatatattatgtcatcgttgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggcgactagaagtctggacctcctcctctatactccctgttaacctctNotISapIBspQI1,4201,4401,4601,4801,5001,5201,5401,560
    agtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtcacttaatatatttatatttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccRRE1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggatgtccggtctgttaataacagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccBbvCIRRE1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaacgagaccttttgagtaaacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcAleI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcacttacttgttcttaataaccttaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtg2,1202,1402,1602,1802,2002,2202,2402,260
    cattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaattttaaaagaagtaatagcaaagtctgggtggagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagccgtgacgcacgcggttaagacgtctgtttaccgtcataagtaggtgttaaaattttcttKflI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttggttaattaatgcaaagatggataaagttttaaattcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctctaggtcaaaccaattaattacgtttctacctatttcaaaatttPacIEF-1a2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaaggtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccatttgaccctttcAgeIEF-1a2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgcctactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggaacgcacggaEF-1a2,8202,8402,8602,8802,9002,9202,9402,960
    tgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccaaccttcacccaccctctcaagctccggaacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggaagcgcggacEF-1a2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgagagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgctgcgaaaaaaagaccgttctatcagaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgtacaagccgcApaIPciIBfuAIBspMIPspOMIEF-1a3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgtccgccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccggccggacgagaccacggaccggagcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggccgggacgacFseIPasIEF-1a3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttagggtccctcgagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgtttccttttcccggaaaggcaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcagcagaaatccTliIXhoIBbvCIPaeR7IEF-1a3,5203,5403,5603,5803,6003,6203,6403,660
    ttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcagaacccccctccccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatccggtcgaaccgtgaactacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaaggtaaagtcEF-1a3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtgtcgtgacGTACGGCCACCatgacagagcagagcgagaggccctacaacggcacctactacaccctggaagataagcacttctgggccgcctttctgaacctggccagacacaacgcctacatcaccctgacacacatcgaccggcagctggcctacagcaaggccgacatcacacagcactgCATGCCGGTGGtactgtctcgtctcgctctccgggatgttgccgtggatgatgtgggaccttctattcgtgaagacccggcggaaagacttggaccggtctgtgttgcggatgtagtgggactgtgtgtagctggccgtcgaccggatgtcgttccggctgtagtBsiWIMT2EQ4SE6RP8YN10GT12YY14TL16ED18KH20FW22AA24FL26NL28AR30HN32AY34IT36LT38HI40DR42QL44AY46SK48AD50I3872-7396PguCas13b3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ccaacgaccaggacgtgctgagcttcaaggccctgtggaagaacttcgacaacgacctggaaagaaagagccggctgcggagcctgatcctgaagcacttcagctttctggaaggcgccgcttacggcaagaagctgttcgagtctaagagcagcggcaacaagagcagcaagaaggttgctggtcctgcacgactcgaagttccgggacaccttcttgaagctgttgctggacctttctttctcggccgacgcctcggactaggacttcgtgaagtcgaaagaccttccgcggcgaatgccgttcttcgacaagctcagattctcgtcgccgttgttctcgtcgttcttBlpIT52ND54QD56VL58SF60KA62LW64KN66FD68ND70LE72RK74SR76LR78SL80IL82KH84FS86FL88EG90AA92YG94KK96LF98ES100KS102SG104NK106SS108KN1103872-7396PguCas13b4,0404,0604,0804,1004,1204,1404,1604,1804,200
    caaagagctgaccaagaaagagaaagaggaactgcaggccaacgctctgagcctggacaacctgaagtctatcctgttcgacttcctgcagaagctgaaggacttccggaactactacagccactaccggcacagcggaagcagcgagctgcctctgttcgacggcaacatgctggtttctcgactggttctttctctttctccttgacgtccggttgcgagactcggacctgttggacttcagataggacaagctgaaggacgtcttcgacttcctgaaggccttgatgatgtcggtgatggccgtgtcgccttcgtcgctcgacggagacaagctgccgttgtacgacKE112LT114KK116EK118EE120LQ122AN124AL126SL128DN130LK132SI134LF136DF138LQ140KL142KD144FR146NY148YS150HY152RH154SG156SS158EL160PL162FD164GN166ML1683872-7396PguCas13b4,2204,2404,2604,2804,3004,3204,3404,360
    cagcggctgtacaacgtgttcgacgtgtccgtgcagcgcgtgaagatcgaccacgagcacaacgatgaggtggaccctcactaccacttcaaccacctcgtgcggaagggcaagaaggacagatacggccacaacgacaaccccagcttcaagcaccacttcgtggacggcgaaggtcgccgacatgttgcacaagctgcacaggcacgtcgcgcacttctagctggtgctcgtgttgctactccacctgggagtgatggtgaagttggtggagcacgccttcccgttcttcctgtctatgccggtgttgctgttggggtcgaagttcgtggtgaagcacctgccgcttcBarIPsrIQR170LY172NV174FD176VS178VQ180RV182KI184DH186EH188ND190EV192DP194HY196HF198NH200LV202RK204GK206KD208RY210GH212ND214NP216SF218KH220HF222VD224GE2263872-7396PguCas13b4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gcatggttacagaagccggcctgctgttcttcgtgtccctgttcctggaaaagcgggacgccatctggatgcagaagaagatccggggcttcaaaggcggcaccgagacataccagcagatgaccaacgaggtgttctgccggtccagaatcagcctgcctaagctgaagctggacgtaccaatgtcttcggccggacgacaagaagcacagggacaaggaccttttcgccctgcggtagacctacgtcttcttctaggccccgaagtttccgccgtggctctgtatggtcgtctactggttgctccacaagacggccaggtcttagtcggacggattcgacttcgacctAjuIGM228VT230EA232GL234LF236FV238SL240FL242EK244RD246AI248WM250QK252KI254RG256FK258GG260TE262TY264QQ266MT268NE270VF272CR274SR276IS278LP280KL282KL284E3872-7396PguCas13b4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aagcctgcggatggacgactggatgctgctggacatgctgaacgaactcgtgcggtgccccaagcctctgtacgacagactgagagaggacgaccgggcctgctttagagtgcccgtggatatcctgcctgacgaggacgatacagatggcggcggagaggaccccttcaagaatttcggacgcctacctgctgacctacgacgacctgtacgacttgcttgagcacgccacggggttcggagacatgctgtctgactctctcctgctggcccggacgaaatctcacgggcacctataggacggactgctcctgctatgtctaccgccgcctctcctggggaagttcttaS286LR288MD290DW292ML294LD296ML298NE300LV302RC304PK306PL308YD310RL312RE314DD316RA318CF320RV322PV324DI326LP328DE330DD332TD334GG336GE338DP340FK342N3872-7396PguCas13b4,7404,7604,7804,8004,8204,8404,8604,8804,900
    accctcgtccggcaccaggacagattcccttacttcgccctgcggtacttcgacctgaagaaggtgttcaccagcctgcggttccacatcgatctgggcacctaccactttgccatctacaagaagatgatcggcgagcagcccgaggacagacacctgaccagaaacctgtacgtgggagcaggccgtggtcctgtctaagggaatgaagcgggacgccatgaagctggacttcttccacaagtggtcggacgccaaggtgtagctagacccgtggatggtgaaacggtagatgttcttctactagccgctcgtcgggctcctgtctgtggactggtctttggacatgcT344LV346RH348QD350RF352PY354FA356LR358YF360DL362KK364VF366TS368LR370FH372ID374LG376TY378HF380AI382YK384KM386IG388EQ390PE392DR394HL396TR398NL400Y3872-7396PguCas13b4,9204,9404,9604,9805,0005,0205,0405,060
    gcttcggccggatccaggacttcgccgaagaacacagacccgaggaatggaagcggctcgtcagagatctggactacttcgagacaggcgacaagccctacatcagccagacaagcccacactaccacatcgagaagggaaagatcggcctgagattcatgcccgagggccagcacgaagccggcctaggtcctgaagcggcttcttgtgtctgggctccttaccttcgccgagcagtctctagacctgatgaagctctgtccgctgttcgggatgtagtcggtctgttcgggtgtgatggtgtagctcttccctttctagccggactctaagtacgggctcccggtcgtBamHIG402FG404RI406QD408FA410EE412HR414PE416EW418KR420LV422RD424LD426YF428ET430GD432KP434YI436SQ438TS440PH442YH444IE446KG448KI450GL452RF454MP456EG458QH4603872-7396PguCas13b5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tctttggccctctccagaagtgggcaccaccagaaccggcagatctaagtacgcccaggacaagagactgaccgccgaggcctttctgtctgtgcacgagctgatgcctatgatgttctactacttcctgctgcgcgagaagtacagcgaggaagtgtctgccgagagagtgcagagaaaccgggagaggtcttcacccgtggtggtcttggccgtctagattcatgcgggtcctgttctctgactggcggctccggaaagacagacacgtgctcgactacggatactacaagatgatgaaggacgacgcgctcttcatgtcgctccttcacagacggctctctcacgtcLW462PS464PE466VG468TT470RT472GR474SK476YA478QD480KR482LT484AE486AF488LS490VH492EL494MP496MM498FY500YF502LL504RE506KY508SE510EV512SA514ER516VQ5183872-7396PguCas13b5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggcagaatcaagcgcgtgatcgaggatgtgtacgccgtgtacgatgcctttgccagggacgagatcaacacccgggatgagctggatgcctgcctggccgataagggcatcagaagaggccatctgcctcgccagatgatcgccatcctgagccaagagcacaaggacatggaagccgtcttagttcgcgcactagctcctacacatgcggcacatgctacggaaacggtccctgctctagttgtgggccctactcgacctacggacggaccggctattcccgtagtcttctccggtagacggagcggtctactagcggtaggactcggttctcgtgttcctgtaccttcBsaBIGR520IK522RV524IE526DV528YA530VY532DA534FA536RD538EI540NT542RD544EL546DA548CL550AD552KG554IR556RG558HL560PR562QM564IA566IL568SQ570EH572KD574ME5763872-7396PguCas13b5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agaagattcggaagaaactgcaagagatgatggccgacaccgaccaccggctggatatgctggatagacagaccgaccggaagatcagaatcggccggaagaatgccggactgcctaagagcggagtgatcgccgattggctcgtgcgggacatgatgagatttcagcccgtggctcttctaagccttctttgacgttctctactaccggctgtggctggtggccgacctatacgacctatctgtctggctggccttctagtcttagccggccttcttacggcctgacggattctcgcctcactagcggctaaccgagcacgccctgtactactctaaagtcgggcaccgBsaBIEK578IR580KK582LQ584EM586MA588DT590DH592RL594DM596LD598RQ600TD602RK604IR606IG608RK610NA612GL614PK616SG618VI620AD622WL624VR626DM628MR630FQ632PV634A3872-7396PguCas13b5,6205,6405,6605,6805,7005,7205,7405,760
    caaggacgccagcggaaagcctctgaacaactccaaggccaacagcaccgagtaccggatgctgcagagagccctggctctgtttggaggcgagaaagagaggctgaccccatacttccggcagatgaatctgaccggcggaaacaaccctcatccttttctgcacgaaactcgcgttcctgcggtcgcctttcggagacttgttgaggttccggttgtcgtggctcatggcctacgacgtctctcgggaccgagacaaacctccgctctttctctccgactggggtatgaaggccgtctacttagactggccgcctttgttgggagtaggaaaagacgtgctttgagcgK636DA638SG640KP642LN644NS646KA648NS650TE652YR654ML656QR658AL660AL662FG664GE666KE668RL670TP672YF674RQ676MN678LT680GG682NN684PH686PF688LH690ET692R3872-7396PguCas13b5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgggagagccacaccaacatcctgtccttttaccggtcctacctgagagcccgcaaggccttcctcgaaaggatcggcagatccgacagagtggaaaaccggccatttctgctgctgaaagagcccaagaccgacagacagacactggtggctggatggaagggcgagttccatcaccctctcggtgtggttgtaggacaggaaaatggccaggatggactctcgggcgttccggaaggagctttcctagccgtctaggctgtctcaccttttggccggtaaagacgacgactttctcgggttctggctgtctgtctgtgaccaccgacctaccttcccgctcaaggtagAgeIW694ES696HT698NI700LS702FY704RS706YL708RA710RK712AF714LE716RI718GR720SD722RV724EN726RP728FL730LL732KE734PK736TD738RQ740TL742VA744GW746KG748EF750H3872-7396PguCas13b5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tgccaagaggcatcttcacagaggccgtgcgggattgcctgatcgagatgggacacgatgaagtggccagctacaaagaagtgggattcatggccaaggccgtgccactgtactttgagagagcctgcgaggacagggtgcagcccttttacgacagccccttcaacgtgggcaaacggttctccgtagaagtgtctccggcacgccctaacggactagctctaccctgtgctacttcaccggtcgatgtttcttcaccctaagtaccggttccggcacggtgacatgaaactctctcggacgctcctgtcccacgtcgggaaaatgctgtcggggaagttgcacccgttL752PR754GI756FT758EA760VR762DC764LI766EM768GH770DE772VA774SY776KE778VG780FM782AK784AV786PL788YF790ER792AC794ED796RV798QP800FY802DS804PF806NV808GN8103872-7396PguCas13b6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cagcctgaagcctaagaagggcagattcctgagcaaagaggaacgggccgaagagtgggagcggggcaaagagagattccgggatctcgaagcctggtcctatagcgccgccagaagaatcgaggacgcctttgccggcatcgagtatgctagccccggaaacaagaagaaaatcgtcggacttcggattcttcccgtctaaggactcgtttctccttgcccggcttctcaccctcgccccgtttctctctaaggccctagagcttcggaccaggatatcgcggcggtcttcttagctcctgcggaaacggccgtagctcatacgatcggggcctttgttcttcttttagBmtINheISL812KP814KK816GR818FL820SK822EE824RA826EE828WE830RG832KE834RF836RD838LE840AW842SY844SA846AR848RI850ED852AF854AG856IE858YA860SP862GN864KK866KI8683872-7396PguCas13b6,3206,3406,3606,3806,4006,4206,4406,460
    gagcagctcctgcgggacctgagcctgtgggaagcctttgagagcaagctgaaagtgcgggccgaccggatcaatctggccaagctgaagaaagaaatcctggaagcccaagaacacccctaccacgacttcaagagctggcagaagttcgagcgcgagctgcggctggtcaagactcgtcgaggacgccctggactcggacacccttcggaaactctcgttcgactttcacgcccggctggcctagttagaccggttcgacttctttctttaggaccttcgggttcttgtggggatggtgctgaagttctcgaccgtcttcaagctcgcgctcgacgccgaccagttctEQ870LL872RD874LS876LW878EA880FE882SK884LK886VR888AD890RI892NL894AK896LK898KE900IL902EA904QE906HP908YH910DF912KS914WQ916KF918ER920EL922RL924VK9263872-7396PguCas13b6,4806,5006,5206,5406,5606,5806,6006,6206,640
    accaggatatcatcacctggatgatgtgccgcgacctgatggaagaaaacaaggtggaaggcctggacaccggcacactgtacctgaaggacatcagacccaacgtgcaagagcagggcagcctgaacgtgctgaacagagtgaagcccatgagactgcccgtggtggtgtacagtggtcctatagtagtggacctactacacggcgctggactaccttcttttgttccaccttccggacctgtggccgtgtgacatggacttcctgtagtctgggttgcacgttctcgtcccgtcggacttgcacgacttgtctcacttcgggtactctgacgggcaccaccacatgtcNQ928DI930IT932WM934MC936RD938LM940EE942NK944VE946GL948DT950GT952LY954LK956DI958RP960NV962QE964QG966SL968NV970LN972RV974KP976MR978LP980VV982VY984R3872-7396PguCas13b6,6606,6806,7006,7206,7406,7606,7806,8006,820
    agccgatagcagaggccacgtgcacaaagaagaagcccctctggccaccgtgtacatcgaggaaagagacaccaagctgctgaagcagggcaacttcaagtccttcgtgaaggacaggcggctgaacggcctgttctccttcgttgatactggcggactggccatggaacagtactcggctatcgtctccggtgcacgtgtttcttcttcggggagaccggtggcacatgtagctcctttctctgtggttcgacgacttcgtcccgttgaagttcaggaagcacttcctgtccgccgacttgccggacaagaggaagcaactatgaccgcctgaccggtaccttgtcatgA986DS988RG990HV992HK994EE996AP998LA1000TV1002YI1004EE1006RD1008TK1010LL1012KQ1014GN1016FK1018SF1020VK1022DR1024RL1026NG1028LF1030SF1032VD1034TG1036GL1038AM1040EQ1042Y3872-7396PguCas13b6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cccatctccaagctgagagtggaatacgagctggccaagtaccagaccgccagagtgtgtgtgttcgagctgaccctgagactggaagagtccctgctgacccggtatcctcatctgcccgacgagagcttcagagagatgctggaatcttggagcgaccctctgctggccaaatgggtagaggttcgactctcaccttatgctcgaccggttcatggtctggcggtctcacacacacaagctcgactgggactctgaccttctcagggacgactgggccataggagtagacgggctgctctcgaagtctctctacgaccttagaacctcgctgggagacgaccggtttaXcmIP1044IS1046KL1048RV1050EY1052EL1054AK1056YQ1058TA1060RV1062CV1064FE1066LT1068LR1070LE1072ES1074LL1076TR1078YP1080HL1082PD1084ES1086FR1088EM1090LE1092SW1094SD1096PL1098LA1100K3872-7396PguCas13b7,0207,0407,0607,0807,1007,1207,1407,160
    ggcctgaactgcacggaaaagtgcggctgctgatcgccgtgcggaatgcctttagccacaatcagtaccctatgtacgacgaggccgtgttcagctccatccggaagtacgatcctagcagccccgacgccatcgaagagagaatgggcctgaatatcgcccaccgcctgtctgaccggacttgacgtgccttttcacgccgacgactagcggcacgccttacggaaatcggtgttagtcatgggatacatgctgctccggcacaagtcgaggtaggccttcatgctaggatcgtcggggctgcggtagcttctctcttacccggacttatagcgggtggcggacagactW1102PE1104LH1106GK1108VR1110LL1112IA1114VR1116NA1118FS1120HN1122QY1124PM1126YD1128EA1130VF1132SS1134IR1136KY1138DP1140SS1142PD1144AI1146EE1148RM1150GL1152NI1154AH1156RL1158SE11603872-7396PguCas13b7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ggaagtgaagcaggccaaagaaaccgtggaacggatcattcaggccggatccCTTCAACTGCCTCCACTTGAAAGACTGACACTGGGTAGTTCCtacccatacgatgttccagattacgcttatccctacgacgtgcctgattatgcatacccatatgatgtccccgactatgccccttcacttcgtccggtttctttggcaccttgcctagtaagtccggcctaggGAAGTTGACGGAGGTGAACTTTCTGACTGTGACCCATCAAGGatgggtatgctacaaggtctaatgcgaatagggatgctgcacggactaatacgtatgggtatactacaggggctgatacggNdeIXcmIBamHIG1S2L3Q4L5P6P7L8E9R10L11T12L13G14S15S16Y17P18Y19D20V21P22D23Y24A25Y26P27Y28D29V30P31D32Y33A34Y35P36Y37D38V39P40D41Y42A437397-7528EV1162KQ1164AK1166ET1168VE1170RI1172IQ1174A3872-7396HA tag 3xPguCas13bHIV NES7,3607,3807,4007,4207,4407,4607,4807,5007,520
    taaGaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataattCttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatEcoRI*44WPRE7,5407,5607,5807,6007,6207,6407,6607,6807,700
    aatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgcttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcgWPRE7,7207,7407,7607,7807,8007,8207,8407,860
    cgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttcctgcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaWPRE7,8807,9007,9207,9407,9607,9808,0008,0208,040
    tcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacctcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggcagctggagctctggatctttttgtacctcgttagtgttcatcgttatgtcgtcgatggttacgactaacacggaTliIXhoIPaeR7IWPRE8,0608,0808,1008,1208,1408,1608,1808,2008,220
    ggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccgatcttcgtgttctcctcctcctccacccaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttcccccctgaccttcccgattaagtgagggttgcttctgttctataggaactagacacctagatKpnIAcc65IBsu36I8,2408,2608,2808,3008,3208,3408,3608,3808,400
    ccacacacaaggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgttacaccctgtgagcctgcatgggatgggtgtgtgttccgatgaagggactaaccgtcttgatgtgtggtcccggtccctagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcgttctcttccatcttcttcggttacttcctctcttgtgggcgaacaatgtgggacactcggacgtaccctac8,4208,4408,4608,4808,5008,5208,5408,560
    gatgacccggagagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcctactgggcctctctcttcataatctcacctccaaactgtcggcggatcgtaaagtagtgtaccgggctctcgacgtaggcctgacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacg3' LTR8,5808,6008,6208,6408,6608,6808,7008,7208,740
    cttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgcgaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcccgggcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacgApaIBclIPmeIPspOMI3' LTR8,7608,7808,8008,8208,8408,8608,8808,9008,920
    cttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagata8,9408,9608,9809,0009,0209,0409,0609,0809,100
    ggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggF1 ori9,1209,1409,1609,1809,2009,2209,2409,260
    ggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggacccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgAloIDrdIF1 ori9,2809,3009,3209,3409,3609,3809,4009,4209,440
    tcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggPsiIF1 ori9,4609,4809,5009,5209,5409,5609,5809,6009,620
    ccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggSexAI9,6409,6609,6809,7009,7209,7409,7609,7809,800
    attctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttgacaattaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcacaactgttaBclIAvrII9,8209,8409,8609,8809,9009,9209,9409,960
    taatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaaccatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccgattagtagccgtatcatatagccgtatcatattatgctgttccactccttgatttggtaccggttcaactggtcacggcaaggccacgagtggcgcgcgctgcagcggcctcgccagctcaagacctggctggccgagcccaagagggccctgaagcacctcctgctgaagcggcMauBISgrAIBssHII9,98010,00010,02010,04010,06010,08010,10010,12010,140
    gtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcacaccaggccctgctgcactgggacaagtagtcgcgccaggtcctggtccaccacggcctgttgtgggaccggacccacacccacgcgccggacctgctcgacatgcggctcaccagcctccagcacaggtgcttgaaggccctgcggaggcccggccggtactggctctagccFseISexAI10,16010,18010,20010,22010,24010,26010,28010,30010,320
    cgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggactgacacgtgctacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccaggctcgtcggcacccccgccctcaagcgggacgcgctgggccggccgttgacgcacgtgaagcaccggctcctcgtcctgactgtgcacgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtc10,34010,36010,38010,40010,42010,44010,46010,48010,500
    cgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcaPsiIBstZ17I10,52010,54010,56010,58010,60010,62010,64010,660
    cgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtlac promoterLacOM13-rev10,68010,70010,72010,74010,76010,78010,80010,82010,840
    gtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaacagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttSapIBspQI10,86010,88010,90010,92010,94010,96010,98011,00011,020
    tcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccDrdIPciIColE1 origin11,04011,06011,08011,10011,12011,14011,16011,18011,200
    cgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacccccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttgggggColE1 origin11,22011,24011,26011,28011,30011,32011,34011,360
    cgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagagcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttctColE1 origin11,38011,40011,42011,44011,46011,48011,50011,52011,540
    acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccaColE1 origin11,56011,58011,60011,62011,64011,66011,68011,70011,720
    ctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcgactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagBspHIColE1 originAmpR11,74011,76011,78011,80011,82011,84011,86011,88011,900
    agcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagatcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtctAhdIAmpR11,92011,94011,96011,98012,00012,02012,04012,060
    agtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaatcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttFspIAmpR12,08012,10012,12012,14012,16012,18012,20012,22012,240
    ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagPvuIScaIAmpR12,26012,28012,30012,32012,34012,36012,38012,40012,420
    aaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaattggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattAmpR12,44012,46012,48012,50012,52012,54012,56012,58012,600
    cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtcgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagSspI12,62012,64012,66012,68012,70012,72012,74012,760
    tcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgBspHI12,78012,80012,82012,84012,860
    • Plasmid
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    12861 bp9000100001100012000100020003000EF-1a

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    12861