Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV-CaMKIIa-eArchT 3.0-EYFP-WPRE
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtttaacattatggccttaggtggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcaaattgtaataccggaatccaMluISbfIAflIIIBsu36IL-ITRa-CaMKII(1.3)20406080100120140160
    cacttcatctccatggggttcttcttctgattttctagaaaatgagatgggggtgcagagagcttcctcagtgacctgcccagggtcacatcagaaatgtcagagctagaacttgaactcagattactaatcttaaattccatgccttgggggcatgcaagtacgatatacagaagtgaagtagaggtaccccaagaagaagactaaaagatcttttactctacccccacgtctctcgaaggagtcactggacgggtcccagtgtagtctttacagtctcgatcttgaacttgagtctaatgattagaatttaaggtacggaacccccgtacgttcatgctatatgtcttPsrISphIXbaIa-CaMKII(1.3)180200220240260280300320340
    ggagtgaactcattagggcagatgaccaatgagtttaggaaagaagagtccagggcagggtacatctacaccacccgcccagccctgggtgagtccagccacgttcacctcattatagttgcctctctccagtcctaccttgacgggaagcacaagcagaaactgggacaggagccctcacttgagtaatcccgtctactggttactcaaatcctttcttctcaggtcccgtcccatgtagatgtggtgggcgggtcgggacccactcaggtcggtgcaagtggagtaatatcaacggagagaggtcaggatggaactgcccttcgtgttcgtctttgaccctgtcctcgAlwNIa-CaMKII(1.3)360380400420440460480500520
    cccaggagaccaaatcttcatggtccctctgggaggatgggtggggagagctgtggcagaggcctcaggaggggccctgctgctcagtggtgacagataggggtgagaaagcagacagagtcattccgtcagcattctgggtctgtttggtacttcttctcacgctaaggtggcggggtcctctggtttagaagtaccagggagaccctcctacccacccctctcgacaccgtctccggagtcctccccgggacgacgagtcaccactgtctatccccactctttcgtctgtctcagtaaggcagtcgtaagacccagacaaaccatgaagaagagtgcgattccaccgcBsmIStuIEcoNIPflFIBsu36ITth111Ia-CaMKII(1.3)540560580600620640660680700
    gtgtgatatgcacaatggctaaaaagcagggagagctggaaagaaacaaggacagagacagaggccaagtcaaccagaccaattcccagaggaagcaaagaaaccattacagagactacaagggggaagggaaggagagatgaattagcttcccctgtaaaccttagaacccagccacactatacgtgttaccgatttttcgtccctctcgacctttctttgttcctgtctctgtctccggttcagttggtctggttaagggtctccttcgtttctttggtaatgtctctgatgttcccccttcccttcctctctacttaatcgaaggggacatttggaatcttgggtcgHincIIa-CaMKII(1.3)720740760780800820840860
    tgttgccagggcaacggggcaatacctgtctcttcagaggagatgaagttgccagggtaactacatcctgtctttctcaaggaccatcccagaatgtggcacccactagccgttaccatagcaactgcctctttgccccacttaatcccatcccgtctgttaaaagggccctataacaacggtcccgttgccccgttatggacagagaagtctcctctacttcaacggtcccattgatgtaggacagaaagagttcctggtagggtcttacaccgtgggtgatcggcaatggtatcgttgacggagaaacggggtgaattagggtagggcagacaattttcccgggatata-CaMKII(1.3)8809009209409609801,0001,0201,040
    gttggaggtgggggaggtaggaagagcgatgatcacttgtggactaagtttgttcgcatccccttctccaaccccctcagtacatcaccctgggggaacagggtccacttgctcctgggcccacacagtcctgcagtattgtgtatataaggccagggcaaagaggagcaggtttcaacctccaccccctccatccttctcgctactagtgaacacctgattcaaacaagcgtaggggaagaggttgggggagtcatgtagtgggacccccttgtcccaggtgaacgaggacccgggtgtgtcaggacgtcataacacatatattccggtcccgtttctcctcgtccaaaBclISapIBspQIa-CaMKII(1.3)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaagtgaaaggcaggcaggtgttggggaggcagttaccggggcaacgggaacagggcgtttcggaggtggttgccatggggacctggatgctgacgaaggctcgcgaggctgtgagcagccacagtgccctgctcagaagccccaagctcgtcagtcaagccggttctccgtttatttcactttccgtccgtccacaacccctccgtcaatggccccgttgcccttgtcccgcaaagcctccaccaacggtacccctggacctacgactgcttccgagcgctccgacactcgtcggtgtcacgggacgagtcttcggggttcgagcagtcagttcggccaagaggcaaaNruIPaqCIPpuMIa-CaMKII(1.3)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gcactcaggagcacgggcaggcgagtggcccctagttctgggggcagctctagagcggtaccggatccccgggtaccggtaggccaccatggacccaattgcactgcaggcggggtatgacctcctcggtgacgggaggcccgagacactctggctgggcatcgggacgcttctccgtgagtcctcgtgcccgtccgctcaccggggatcaagacccccgtcgagatctcgccatggcctaggggcccatggccatccggtggtacctgggttaacgtgacgtccgccccatactggaggagccactgccctccgggctctgtgagaccgacccgtagccctgcgaagagAgeIKpnIKpnIMfeIXbaIBamHIAcc65IAcc65IArch-Ta-CaMKII(1.3)1,4201,4401,4601,4801,5001,5201,5401,560
    atgctcatcggaacgttttacttcatcgtcaaaggatggggtgtaacagacaaggaagcgcgcgagtactactcgatcactattcttgtgccgggcatcgcctcagcggcgtatttgagcatgttctttggcatcgggctgaccgaagtgacggtagcgggagaggtcttggatatacgagtagccttgcaaaatgaagtagcagtttcctaccccacattgtctgttccttcgcgcgctcatgatgagctagtgataagaacacggcccgtagcggagtcgccgcataaactcgtacaagaaaccgtagcccgactggcttcactgccatcgccctctccagaacctatScaIBbvCIEcoRVArch-T1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tctactatgcgcgctacgcagactggctgtttacaactccgctccttttgcttgatcttgctctgttggcaaaagtagatagggtgtcgattgggacgctggtgggagtagatgcccttatgattgtcacgggattgattggggcattgtcgcacacgcctctcgcccgatattcagatgatacgcgcgatgcgtctgaccgacaaatgttgaggcgaggaaaacgaactagaacgagacaaccgttttcatctatcccacagctaaccctgcgaccaccctcatctacgggaatactaacagtgccctaactaaccccgtaacagcgtgtgcggagagcgggctataagArch-T1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtggtggttgttttccaccatctgcatgatcgtggtcctctacttcttggcgacgagccttcgggctgcggcgaaggagagaggacccgaggtcgcgtccacattcaacaccttgactgccctggtgctggtgctctggaccgcgtatcccatcttgtggattatcggaactgaacaccaccaacaaaaggtggtagacgtactagcaccaggagatgaagaaccgctgctcggaagcccgacgccgcttcctctctcctgggctccagcgcaggtgtaagttgtggaactgacgggaccacgaccacgagacctggcgcatagggtagaacacctaatagccttgacttPpuMIArch-T1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggtgccggggtcgtcggtctgggaatcgaaacactcctcttcatggtgttggacgtaacagccaaggtcggatttggtttcatcttgctgcggtcaagagcaattcttggggatacggaagccccggagcctgcggccgccaagagcaggatcaccagcgagggcgagtacatccccacggccccagcagccagacccttagctttgtgaggagaagtaccacaacctgcattgtcggttccagcctaaaccaaagtagaacgacgccagttctcgttaagaacccctatgccttcggggcctcggacgccggcggttctcgtcctagtggtcgctcccgctcatgtaggArch-TTS2,1202,1402,1602,1802,2002,2202,2402,260
    ccctggaccagatcgacatcaacgtggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccacgggacctggtctagctgtagttgcaccactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtgBsaBIEYFP (no start)TS2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagaccgccgttcgacgggcacgggaccgggtgggagcactggtggaagccgatgccggacgtcacgaagcgggcgatggggctggtgtacttcgtcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctggEYFP (no start)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagagcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgtgttcgacctcatgttgatgttgtcggtgttgcagatatagtaccggctgttcgtcttcttgccgtagttccacttgaagttctEYFP (no start)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgacaggcggtgttgtagctcctgccgtcgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctgttggtgatggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactgEYFP (no start)2,8202,8402,8602,8802,9002,9202,9402,960
    cgccgccgggatcactctcggcatggacgagctgtacaagttctgctacgagaacgaggtgtaatgagaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgcggcggccctagtgagagccgtacctgctcgacatgttcaagacgatgctcttgctccacattactcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgaClaIBspDIBsrGIEcoRIEcoRVHindIIIWPREEYFP (no start)ER export2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtWPRE3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggacacaacggtgWPRE3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagAfeIBbsIClaINaeITliIXhoIZraIAatIIBspDISacIINgoMIVPaeR7IWPRE3,5203,5403,5603,5803,6003,6203,6403,660
    gagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaagggctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttcccBglIICspCIhGH polyA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttcgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaBbsIBlpINsiISphIBbvCIhGH polyA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggAloIPmlIBsaAIBstXIRsrIIBstEIIhGH polyA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtaKasINarISbfISfoIPluTIR-ITR4,2204,2404,2604,2804,3004,3204,3404,360
    ctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggcttgacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaNaeINgoMIVf1 Origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacAloIBsaAIDraIIIf1 Origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgaPsiIf1 Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagca4,9204,9404,9604,9805,0005,0205,0405,060
    gatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataacttttZraIAatII5,0805,1005,1205,1405,1605,1805,2005,2205,240
    aggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgtccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaacAmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    agagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggtctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccScaIXmnIAmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    catgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccagtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtPvuIAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    aacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgacAseIFspIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtctatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    agaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatccttctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggapUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttctpUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    actctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgactgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctgAlwNIpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctcgatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagpUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori6,8406,8606,8806,9006,9206,940
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6940 bp500055006000650050010001500Arch-TL-ITR,SbfI,AflIII,MluI,Bsu36I,XbaI,PsrI,SphIAlwNI,EcoNI,StuI,Bsu36I,PflFI,Tth111I,BsmIHincIIa-CaMKII(1.3)BspQI,SapI,BclIPaqCI,PpuMI,NruIXbaI,Acc65I,KpnI,BamHI,Acc65I,AgeIKpnI,MfeIScaIBbvCIXmnIScaIAmpR,PvuIFspIAseIpUC ori,AlwNI,AflIII,PciI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    6940