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pTI_O1
  • Sequence Map
Editing disabled on read-only sequence.
cagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacgtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgAccITatIBsaAIPflFIBstZ17ITth111IPJRPFF20406080100120140160
acccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacatgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtBcgIPfoIXmnIROP180200220240260280300320340
gatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgactacggaggcacattccccctaaagacaagtacccccattactatggctactttgctctctccROPROP360380400420440460480500520
atgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatacgagtgctatgcccaatgactactacttgtacgggccaatgaccttgcaacactcccatttgttgaccgccatacctacgccgccctggtctctttttagtgagtcccagttacggtcgcgaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgtaggacgctAfeIBsmFI540560580600620640660680700
tgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccacgtctaggccttgtattaccacgtcccgcgactgaaggcgcaaaggtctgaaatgctttgtgcctttggcttctggtaagtacaacaacgagtccagcgtctgcaaaacgtcgtcgtcagcgaagtgcaagcgagcgcatagccactaagtaagacgattggtcattccgttggBsaBIBspEIBpu10I720740760780800820840860
ccgccagcctagccgggtcctcaacgacaggagcacgatcatgctagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagggcggtcggatcggcccaggagttgctgtcctcgtgctagtacgatcagtacggggcgcgggtggccttcctcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcPpuMIEcoO109I*364QG362SE360L1033-2124LacI8809009209409609801,0001,0201,040
tcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacgKasINarISfoIPluTIR358SV356QR354AL352QM350LS348DA346LA344RP342SA340TQ338TN336PA334LT332TK330RK328VL326SV324PL322LQ320NG318KV316AQ314GQ312SL310QL308LR306DV304ST302Q1033-2124LacI1,0601,0801,1001,1201,1401,1601,1801,2001,220
cccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcatagggtgatggctctacaggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcaccHpaIBssHIIHincIIG300LL298RF296DQ294KI292TT290LP288PI286YC284SS282DE280TD278DY276GV274VS272ID270AG268VR266LG264SE262TI260AR258MA256GL254AM252QD250NA248VL246MA244TP2421033-2124LacI1,2401,2601,2801,3001,3201,3401,3601,3801,400
gaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgcttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacApaIBcgIPspOMINmeAIIIVI240GE238NL236MQ234MT232QQ230FG228SM226AS224WD222GE220RE218AI216PQ214IQ212NR210TL208YK206HW204GA202LR200LR198AS196VS194SL192PG190AL188LA186IQ1841033-2124LacI1,4201,4401,4601,4801,5001,5201,5401,560
ctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagBclIBstEIIQH182GL180AV178LH176EV174GL172RT170GD168EH166SF164II162SN160IP158TQ156DS154VD152LF150LA148PV146NT144CA142AE140VA138IA136DQ134DD132LP130YN128II1261033-2124LacI1,5801,6001,6201,6401,6601,6801,7001,7201,740
agcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttMluIAflIIILG124SV122RQ120AL118LN116HV114AA112KC110AE108VG106SR104EV102MS100VV98VS96AG94LQ92DA90RS88KI86AA84VI82QS80PA78HL76AL74SS72TA70VG68I1033-2124LacI1,7601,7801,8001,8201,8401,8601,8801,9001,920
tcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataaagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattBstAPIL66LS64QK62GA60LQ58QA56VR54NP52IY50NL48EA46MA44AE42VK40ER38TK36AS34VH32SA30QN28VV26RS24VT22QY20SV18GA16YE14AV12DY10L1033-2124LacI1,9401,9601,9802,0002,0202,0402,0602,0802,100
cgttactggtttcacATTCACCACcctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgcgcaatgaccaaagtgTAAGTGGTGggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcgPfoIEcoNIT8VP6KV4NV2V1033-2124LacI promoterVUNFLacI2,1202,1402,1602,1802,2002,2202,2402,260
cgcaaggaatggtgaattGCTCTTCttaagCATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTCCGagctcttcaatgaggagtgagaccattgacagcGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTgcgttccttaccacttaaCGAGAAGaattcGTAATGAGCGTAGGTAAGAGTCCGACAGAGCAGAGCAGAGGCtcgagaagttactcctcactctggtaactgtcgCGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGABsaISacIAflIIBseRIEco53kILacZ casseteUNS1pLac2,2802,3002,3202,3402,3602,3802,4002,4202,440
CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGTTCGAACGTACGGACGTCCAGCTGAGATCTCCTAGGGGCCCATGGCTCGAGCTTAAGTGACCGAccIAvaIKpnIPstISacISalISbfISmaISphIXbaIXmaIBamHIBfuAIBsoBIBspMIBsrBIEcoRITspMIAcc65IHincIIEco53kIHindIIIMT2MI4TP6SL8HA10CR12ST14LE16DP18RV20PS22SN24SL26ALacZaLacZ cassetepLacLacZ alpha2,4602,4802,5002,5202,5402,5602,5802,6002,620
CGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGGACTACGCCATAAAAGAGGAABglIFspIKasINarIPvuISfoIPluTIV28VL30QR32RD34WE36NP38GV40TQ42LN44RL46AA48HP50PF52AS54WR56NS58EE60AR62TD64RP66SQ68QL70RS72LN74GE76WR78LM80RY82FL84LLacZaLacZ casseteLacZ alpha2,6402,6602,6802,7002,7202,7402,7602,7802,800
ACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACactagtgagcacggtctTGCGTAGACACGCCATAAAGTGTGGCGTATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGtgatcactcgtgccagaNdeISpeITatIBstAPIT86HL88CG90IS92HR94IW96CT98LS100TI102CS104DA106A*108LacZaLacZ casseteLacZ alpha2,8202,8402,8602,8802,9002,9202,9402,960
cacgctagcatgaagagcaCCCCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACCcgccggcgaggcccacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcgtgcgatcgtacttctcgtGGGGTCCTATGTATCTAATGGTGTTGAGGCTCGGGAAGGTGGgcggccgctccgggtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagAloIBglIBmtIBsaIMreINaeINheIBsaAISgrAIDraIIINgoMIVUNSXVunsXR2,9803,0003,0203,0403,0603,0803,1003,1203,140
aaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttctggcggcacgatggcatgagattatcaaaaaggatcttcacctagatttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaagaccgccgtgctaccgtactctaatagtttttcctagaagtggatctaPsiISspI3,1603,1803,2003,2203,2403,2603,2803,3003,320
ccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagGCTAGTATTATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcCGATCATAATAATCTTTTTGAGTAGCTCGTAGTTTACTTTGACGTTAAATAAGTATAGTCCTAATAGTTATGGTATAAAAACTTTTTCGGCAAAGACATTACTTCCTCTDraIDraI**272FF270ED268LM266LH264FQ262LK260NM258DP256ND254IG252YK250QF248LR246KQ244LS242PS2403399-4217BBa_G0005KanR geneBBa_P1003BBa_P10073,3403,3603,3803,4003,4203,4403,4603,4803,500
AAACTCACCGAGGCAGTTCCAAAGAATGGCAAGGTCCTGGTAACGGTCTGCGATTCCGACCCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAGAGCTTGTGCATTTTTGAGTGGCTCCGTCAAGGTTTCTTACCGTTCCAGGACCATTGCCAGACGCTAAGGCTGGGCAGGTTGTAGTTATGTTGGATAATTAAAGGGGAGCAGTTTTTATTCCAATAGTTCACTCTTTAGTGGTACTCACTGCTGACTTAGGCCACTCTTACCGTTCTCGAACACGTAAPpuMIEcoO109IFE238GL236CN234WL232IA230LD228QY226RD224AI222GV220RG218VD216IC214GI212LK210GE208DF206IL204ND202LS200FD198GH196TV194VS192DP190SF188PL186LK184HM1823399-4217BBa_G0005KanR geneBBa_P1003BBa_P10073,5203,5403,5603,5803,6003,6203,6403,660
TCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATGCGTGATTGCGCCTGAGCAAGACGAAATACACGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGTAACCGGCGCAGGAACACGGCCAGCGAGAAAGGTCTGAACAAGTTGTCCGGTCGGTAATGCGAGCAGTAGTTTTAGTGAGCGTAGTTGGTTTGGCAATAAGTACGCACTAACGCGGACTCGTTCTGCTTTATGTGCTAGCGACAATTTTCCTGTTAATGTTTGTCCTTAGCTTACATTGGCCGCGTCCTTGTGCCGGTCGCAjuIPvuIBpu10IEK180WV178QE176VP174WG172NR170ED168DF166DS164AD162VL160GN158NM156RS154QA152QA150LR148FV146RD144SN142FP140CN138CV136PI134SH132LR130RL128FV126AL124A3399-4217BBa_G0005KanR geneBBa_P1003BBa_P10073,6803,7003,7203,7403,7603,7803,8003,8203,840
CATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAAGGCTGTTTTCCCAGGAATCGCGGTGGTGAGTAACCACGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGGAGAGGCATAAACTCCGTCAGCCAGTTGAGACGGACCATCTCATCTGTAACGTAGTTGTTATAAAAGTGGACTTAGTCCTATAAGAAGATTATGGACCTTCCGACAAAAGGGTCCTTAGCGCCACCACTCATTGGTGCGTAGTAGTCCTCATGCCTATTTTACGAACTACCAGCCCTCTCCGTATTTGAGGCAGTCGGTCAACTCTGCCTGGTAGAGTAGACATTGSspIEcoNID122VI120NE118GS116DP114YE112EL110VQ108FA106TK104GP102IA100TT98LL96WA94DD92PT90RI88FH86KI84TP82LP80MF78ET76LW74NL72RV70ME68DT66V3399-4217BBa_G0005KanR geneBBa_P1003BBa_P10073,8603,8803,9003,9203,9403,9603,9804,0004,020
ATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCGTCCATGTTGGAGTTTAAGCGCGGACGGGAGCAAGACGTTTCCTAGTAACCGTTGCGATGGAAACGGTACAAAGTCTTTGTTGAGACCGCGTAGCCCGAAGGGTATGTTCGCTATCTAACAGCGTGGACTAACGGGCTGTAATAGCGCTCGGGTAAATATGGGTATATTTAGTCGCAGGTACAACCTCAAATTCGCGCCTGCCCTCGTTCTGCAAAGGNruID64NA62VS60GK58GH56KL54FL52EP50AD48PK46GY44LR42YI40TA38GS36QG34VN32DR30AW28KY26GY24LD22AD20MN18SN16LR14PR12SC10ST8E3399-4217BBa_G0005KanR geneBBa_P1003BBa_P10074,0404,0604,0804,1004,1204,1404,1604,1804,200
CGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCtatttagaaGCAACTTATACCGAGTATTGTGGGGAACATAATGACAAATACATTCGTCTGTCAAAATAACAAGTACTACTATATAAAAATAGAACACGTTACATTGTAGTCTCTAAAACTCTGTGTTGCACCGAAACAACTTATTTAGCTTGAAAACGACTCAACTTCCTAGTCGataaatcttBspHIDraIIIR6QI4HS2M3399-4217BBa_P1003BBa_P1007BBa_P1007 cm_promBBa_G0005KanR gene4,2204,2404,2604,2804,3004,3204,3404,360
aaataaacaaataggtcatgaCCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAtttatttgtttatccagtactGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTBspHIpBR3224,3804,4004,4204,4404,4604,4804,5004,5204,540
AGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCAcuIAlwNIpBR3224,5604,5804,6004,6204,6404,6604,6804,7004,720
TGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTpBR3224,7404,7604,7804,8004,8204,8404,8604,8804,900
GGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAABssSIBssSαIpBR322pBR322 ORI4,9204,9404,9604,9805,0005,0205,0405,060
TTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcaPciIBsrBIAflIIIpBR322pBR322 ORI5,0805,1005,1205,1405,1605,1805,200
  • Plasmid
Editing disabled on read-only sequence.
5215 bp37504000425045004750500025050075010001250LacIBBa_P1003BBa_P1007KanR geneBbsIpBR322ROPROPSapI1033-21243399-4217UXF (DNA)UXR (DNA)PFF,PJR,TatI,AccI,BstZ17I,BsaAI,PflFI,Tth111IPfoIBcgIpETm_F (DNA),pETm_R (DNA),XmnIBsmFI,AfeIBsaBI,BspEIBbsILac1 (DNA),Bpu10IEcoO109IPpuMIKasINarISfoI,PluTIlac2 (DNA)HincII,HpaINruISilent Mut: G to C mutation to eliminateXhoI siteBBa_P1007 cm_prom,BspHI,DraIIIBBa_G0001BspHIAcuIAlwNIBssSI,BssSαIColE1, pBR322 ori,pBR322 ORI,AflIII,PciI,BsrBI

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5215