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LSM0252 (PB)EF1a-FKBP-ADARd22-IRESv0-PuroR-P2A-BFP
  • Sequence Map
    Editing disabled on read-only sequence.
    tgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccagAhdIBsaIT286*K284T*282GY280NG278SE276GD274HL272YS270RY268PL266A*264WR262AG260TS258CH256YR254SV252WA250*R248SW246I*244*C242YV240LW238GS236PG234LA232ST230T1-9428: Region 1same as 250/2512Amp20406080100120140160
    ctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtgacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaFspIR228CS226*G224GG222DL220RN218IT216AP214FS212SY210TT208RW206NI204TQ202AV200NN198GN196SC194AD192HH190*A188RR186KT184HS182*E180AG178TG176LS174*P172SN1701-9428: Region 1same as 250/2512Amp180200220240260280300320340
    tacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcScaICS168GG166HQ164AF162FR160NA158GE156TR154RD152ND150ST148LQ146GC144H*142*E140HN138HC136CQ134MI132RK130SD128HW126GY124SA122KR120HS118TL116V*114GL1121-9428: Region 1same as 250/2512Amp360380400420440460480500520
    tcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgag*E110SF108LT106HP104SR102TA100RA98RR96*Y94PI92IG90RW88MA86SS84*F82HE80DN78SF76TR74RP72SF70E*68PD66*R64QQ62SG60TR58HL56GS541-9428: Region 1same as 250/2512Amp540560580600620640660680700
    gtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcTC52GV50SR48*C46RK44SE42GA40NR38PS36CF34CS32PL30IG28CF26LS24YP22RC20PF18TS16YE14D1-9428: Region 1same as 250/2512Amp720740760780800820840860
    cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttatagcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatPsiICspCIsame as 250/25128809009209409609801,0001,0201,040
    aatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgAloIsame as 250/25121,0601,0801,1001,1201,1401,1601,1801,2001,220
    actaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcsame as 250/25121,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcctccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcgcggaFspIsame as 250/25121,4201,4401,4601,4801,5001,5201,5401,560
    cgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattgtgacgtacgttaaagataatcatgtgtaaaattggcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacactgcatgcaatttctattagtacacattttaacBsiWI5' PB ITR Long5' PB ITR shortjt1009same as 250/25121,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacaaaaactcaaaatttcttctataaagtaacaaaatgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgtttttgagttttaaagaagatatttcattgtttt5' PB ITR Longsame as 250/25121,7601,7801,8001,8201,8401,8601,8801,9001,920
    cttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcggaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcSrfIRsrIIcore insulsame as 250/25121,9401,9601,9802,0002,0202,0402,0602,0802,100
    ctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccactagtTTCGCACGATTGCACCTTGGGAGCCTTAACCGCTTTTGAATGTCCGTCTTAGTTAATCCCTAATTGAACGCTCCAAAAGGTGACTTCCAATAgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgatcaAAGCGTGCTAACGTGGAACCCTCGGAATTGGCGAAAACTTACAGGCAGAATCAATTAGGGATTAACTTGCGAGGTTTTCCACTGAAGGTTATSfiISpeIattD Cp36core insulForward Targettarget ...primersame as 250/25122,1202,1402,1602,1802,2002,2202,2402,260
    GGGATTTATTCCTTTTAAAATTAACGGCATAATCGTGCCGCCGAATGCAACCTCGATCGAGCCGGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACCCCTAAATAAGGAAAATTTTAATTGCCGTATTAGCACGGCGGCTTACGTTGGAGCTAGCTCGGCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGAgeIMfeIEcoNIEF-1α promoterattD Cp36Primer 1Reverse TargetTarget ...Primerprime...F-1asame as 250/25122,2802,3002,3202,3402,3602,3802,4002,4202,440
    TGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAASacIIEF-1α promotersame as 250/25122,4602,4802,5002,5202,5402,5602,5802,6002,620
    GCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAAflIIEF-1α promotersame as 250/25122,6402,6602,6802,7002,7202,7402,7602,7802,800
    CGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTAApaIBglIISacIIPspOMIEF-1α promotersame as 250/25122,8202,8402,8602,8802,9002,9202,9402,960
    GTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCFseIBstAPIEF-1α promotersame as 250/25122,9803,0003,0203,0403,0603,0803,1003,1203,140
    CCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCBbvCIEcoNIBpu10IEF-1α promoterfrag1same as 250/25123,1603,1803,2003,2203,2403,2603,2803,3003,320
    TCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGEF-1α promoterfrag1RF from EF-1asame as 250/25123,3403,3603,3803,4003,4203,4403,4603,4803,500
    CATTTCAGGTGTCGTGAATCTAGAAGCCACCatggctagcATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATTCCTCCCGGGACAGAAACAAGGTAAAGTCCACAGCACTTAGATCTTCGGTGGtaccgatcgTACCCTCACGTCCACCTTTGGTAGAGGGGTCCTCTGCCCGCGTGGAAGGGGTTCGCGCCGGTCTGGACGCACCACGTGATGTGGCCCTACGAACTTCTACCTTTCTTTAAACTAAGGAGGGCCCTGTCTTTGTTCBmtINcoINheIXbaIXcmIBsmBIEsp3IMA2SM4GV6QV8ET10IS12PG14DG16RT18FP20KR22GQ24TC26VV28HY30TG32ML34ED36GK38KF40DS42SR44DR46NK483532-5199XZ135FKBPfrom AV132EF-1α...oterfrag1RF fr...F-1ahomologsame as 250/25123,5203,5403,5603,5803,6003,6203,6403,660
    CCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCGGGAAATTCAAATACGATCCGTTCGTCCTCCACTAGGCTCCGACCCTTCTTCCCCAACGGGTCTACTCACACCCAGTCTCTCGGTTTGACTGATATAGAGGTCTAATACGGATACCACGGTGACCCGTGGGTCCGTAGTAGGGTGGTGTACGGTGAGAGCAGAAGCTACACCTCGPF50KF52ML54GK56QE58VI60RG62WE64EG66VA68QM70SV72GQ74RA76KL78TI80SP82DY84AY86GA88TG90HP92GI94IP96PH98AT100LV102FD104VE1063532-5199XZ135FKBPfrom AV1323,6803,7003,7203,7403,7603,7803,8003,8203,840
    TTCTAAAACTGGAAggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCAAGATTTTGACCTTccgcggccctgtcctcccagacccccaccgtgaccgcctagacctcctctgatgttcctgctactgctgttgccgcctagcccccccagaccaccatcaccgccaagcccccctccatggccaAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGAgeIKpnIPsrIAcc65ILL108KL110EG112AG114TG116GS118GG120GT122GG124SG126GD128YK130DD132DD134NG136GS138GG140SG142GS144GG146SG148GG150TG152LH154LD156QT158PS160RQ162PI164P3532-5199XZ135from 250 new pcrhADAR2 cat domain4FKBPfrom...1323,8603,8803,9003,9203,9403,9603,9804,0004,020
    CAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATBstEIIS166EG168LQ170LH172LP174QV176LA178DA180VS182RL184VL186GK188FG190DL192TD194NF196SS198PH200AR202RK204VL206AG208VV210MT212TG214TD216VK218DA220KV222I3532-5199from 250 new pcrhADAR2 cat domain44,0404,0604,0804,1004,1204,1404,1604,1804,200
    AGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGATCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTBclIS224VS226TG228TK230CI232NG234EY236MS238DR240GL242AL244ND246CH248AE250II252SR254RS256LL258RF260LY262TQ264LE266LY268LN270NK272DD274QK276RS278IF280Q3532-5199from 250 new pcrhADAR2 cat domain44,2204,2404,2604,2804,3004,3204,3404,360
    AATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGBsrGIK282SE284RG286GF288RL290KE292NV294QF296HL298YI300ST302SP304CG306DA308RI310FS312PH314EP316IL318EE320PA322DR324HP326NR328KA330RG332QL334RT336KI338ES3403532-5199from 250 new pcrhADAR2 cat domain44,3804,4004,4204,4404,4604,4804,5004,5204,540
    TGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGBlpIBamHIGQ342GT344IP346VR348SN350AS352IQ354AW356DG358VL360QG362ER364LL366TM368SC370SD372KI374AR376WN378VV380GI382QG384SL386LS388IF390VE392PI394YF396SS3983532-5199from 250 new pcrhADAR2 cat domain4homolog4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGATAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTPpuMIHincIIII400LG402SL404YH406GD408HL410SR412AM414YQ416RI418SN420IE422DL424PP426LY428TL430NK432PL434LS436GI438SN440AE442AR444QP446GK448AP450NF452SV454NW4563532-5199from 250 new pcrhADAR2 cat domain44,7404,7604,7804,8004,8204,8404,8604,8804,900
    CGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGMluITV458GD460SA462IE464VI466NA468TT470GK472DE474LG476RA478SR480LC482KH484AL486YC488RW490MR492VH494GK496VP498SH500LL502RS504KI506TK508PN510VY512HE514S3532-5199from 250 new pcrhADAR2 cat domain44,9204,9404,9604,9805,0005,0205,0405,060
    CAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCTGATAGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGACTATCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTK516LA518AK520EY522QA524AK526AR528LF530TA532FI534KA536GL538GA540WV542EK544PT546EQ548DQ550FS552LT554P*5563532-5199from 250 new pcr4hADAR2 cat domainEMCV IRES SitePCRhomolog5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACApaIAvrIIPspOMIfrom 250 new pcr4EMCV IRES Site5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGPmlIHindIIIfrom 250 new pcr4EMCV IRES Site5,4405,4605,4805,5005,5205,5405,5605,5805,600
    TCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTaCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCAtGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATKpnIBmgBIPflMIAcc65Ifrom 250 new pcr4EMCV IRES Site5,6205,6405,6605,6805,7005,7205,7405,760
    ATATGGCCACAACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTTATACCGGTGTTGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGABsiWIRsrIIBstEIIMT2EY4KP6TV8RL10AT12RD14DV16PR18AV20RT22LA24AA26FA28DY30PA32TR34HT36VD38PD40RH42IE44RV46TE48LQ50EL52FL545790-71631PuroRfrom 250 new pcr4PR_S030overlapPR_S029EMCV...itesame as 250/2515,7805,8005,8205,8405,8605,8805,9005,9205,940
    CACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGTR56VG58LD60IG62KV64WV66AD68DG70AA72VA74VW76TT78PE80SV82EA84GA86VF88AE90IG92PR94MA96EL98SG100SR102LA104AQ106QQ108ME110GL1125790-71631PuroRsame as 250/2515,9605,9806,0006,0206,0406,0606,0806,1006,120
    CTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGABsaIEagILA114PH116RP118KE120PA122WF124LA126TV128GV130SP132DH134QG136KG138LG140SA142VV144LP146GV148EA150AE152RA154GV156PA158FL160ET162SA164PR166NL168PF1705790-71631PuroRsame as 250/2516,1406,1606,1806,2006,2206,2406,2606,2806,300
    ACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagagctgatTGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctcgactaNcoISapIBamHIBmgBIBspQISexAIBstAPIYE172RL174GF176TV178TA180DV182EV184PE186GP188RT190WC192MT194RK196PG198AA200TN202FS204LL206KQ208AG210DV212EE214NP216GP218GS220MV222SK224GE226EL228I5790-71631PuroRP2AmtagBFP2homologoushomologsame as 250/2516,3206,3406,3606,3806,4006,4206,4406,460
    taaggagaacatgcacatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctcattcctcttgtacgtgtacttcgacatgtacctcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagBsrGIK230EN232MH234MK236LY238ME240GT242VD244NH246HF248KC250TS252EG254EG256KP258YE260GT262QT264MR266IK268VV270EG272GP274LP276FA278FD280IL282AT284SF286L5790-71631mtagBFP2same as 250/2516,4806,5006,5206,5406,5606,5806,6006,6206,640
    tacggcagcaagaccttcatcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcaatgccgtcgttctggaagtagttggtgtgggtcccgtaggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtBsu36IY288GS290KT292FI294NH296TQ298GI300PD302FF304KQ306SF308PE310GF312TW314ER316VT318TY320ED322GG324VL326TA328TQ330DT332SL334QD336GC338LI340YN342VK344I5790-71631mtagBFP2same as 250/2516,6606,6806,7006,7206,7406,7606,7806,8006,820
    gaggggtgaacttcacatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatcctccccacttgaagtgtaggttgccgggacactacgtcttcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctagAloIR346GV348NF350TS352NG354PV356MQ358KK360TL362GW364EA366FT368ET370LY372PA374DG376GL378EG380RN382DM384AL386KL388VG390GS392HL394IA396NA398KT400TY402RS4045790-71631mtagBFP2same as 250/2516,8406,8606,8806,9006,9206,9406,9606,9807,000
    caagaaacccgctaagaacctcaagatgcctggcgtctactatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAGAATTCCTCGAGgttctttgggcgattcttggagttctacggaccgcagatgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTCTTAAGGAGCTCAccIPacIAlwNIEcoRIPspXIKK406PA408KN410LK412MP414GV416YY418VD420YR422LE424RI426KE428AN430NE432TY434VE436QH438EV440AV442AR444YC446DL448PS450KL452GH454KL456N*4585790-71631mtagBFP2same as 250/2517,0207,0407,0607,0807,1007,1207,1407,160
    CTAGAGCTcGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGATCTCGAgCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCBclI1bGH poly(A) signalsame as 250/2517,1807,2007,2207,2407,2607,2807,3007,3207,340
    GGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACGACGTCAGGTGGCACTTTTCgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTGCTGCAGTCCACCGTGAAAAGctatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagSphIEcoRV13' PB ITR longbGH poly(A) signalsame as 250/2517,3607,3807,4007,4207,4407,4607,4807,5007,520
    acgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttctt13' PB ITR longsame as 250/2517,5407,5607,5807,6007,6207,6407,6607,6807,700
    tgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaaNsiISphI13' PB ITR long3' PB ITR shortjt1010same as 250/2517,7207,7407,7607,7807,8007,8207,8407,860
    gccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgt1same as 250/2517,8807,9007,9207,9407,9607,9808,0008,0208,040
    tcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcaagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagt1same as 250/2518,0608,0808,1008,1208,1408,1608,1808,2008,220
    tggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaa1same as 250/2518,2408,2608,2808,3008,3208,3408,3608,3808,400
    aatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgttacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacacSapIBspQI1same as 250/2518,4208,4408,4608,4808,5008,5208,5408,560
    agcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcg1same as 250/25128,5808,6008,6208,6408,6608,6808,7008,7208,740
    tctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggcsame as 250/25128,7608,7808,8008,8208,8408,8608,8808,9008,920
    gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaacattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttAlwNIsame as 250/25128,9408,9608,9809,0009,0209,0409,0609,0809,100
    gccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcasame as 250/25129,1209,1409,1609,1809,2009,2209,2409,260
    taagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatcattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagL12A*10DT8LC6RD4*R2D1-9428: Region 2same as 250/2512Amp9,2809,3009,3209,3409,3609,3809,4009,420
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9428 bp70007500800085009000500100015002000same as 250/2511EF-1α promoterPrimer 1primer from EF-1a3' PB ITR shortjt10102Amp1-9428: Region 11-9428: Region 25790-7163oLSM0661 (DNA)XZp018.4 (DNA)XZp044.4 (DNA)oLSM0085 (DNA)pUC_oriR_Rv2 (DNA)oLSM-pUC_ori_F (DNA)oLSM-pUC_ori_R (DNA)pUC_oriR_Fv2 (DNA)AhdI,BsaI,FspIScaICspCI,PsiIAloIFspIoverlapjt10095' PB ITR short,BsiWI5' PB ITR LongRsrIIcore insul,SrfIForward Target,target forward primer,SfiI,SpeIattD Cp36Reverse TargetTarget Reverse PrimerAlwNIhomolog,XZp044.5 (DNA),PacI,EcoRI,PspXI,BclIbGH poly(A) signaloLSM0086 (DNA)SphI,EcoRV3' PB ITR longNsiISphIBspQISapIAlwNI

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    9428