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YH001 CMV-TO-tm-GS+XTEN-ADAR2(DD-E396A)-RXR (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBIBsmBIEsp3ISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatggcgctccctgtcaccgcactgcttcttccgctggcactgctgctgcacgctgcacggcctggatccctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtaccgcgagggacagtggcgtgacgaagaaggcgaccgtgacgacgacgtgcgacgtgccggacctagggaccagcaccaacgacctcaggacaaaacaataccgaacgatcactgBsgIBsgIPmeIAflIIBamHIHindIIIG1S2L1V2V3V4A5G6V7L8F9C10Y11G12L13L14V15T16829-891M1A2L3P4V5T6A7L8L9L10P11L12A13L14L15L16H17A18A19R20P21760-822CD8α signal peptideCD28-TMDfrom XG 205CMV promot...th TetOs)720740760780800820840860
    agtggctctttgtgttgaaaattcCGGGTCAGGCGGTTCCGGCAGCGGGGCGGGTAGCGGGTCCAGTGGGTCAGAAACACCCGGCACGAGTGAGTCAGCAACGCCAGAGAGTgctagcGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGtcaccgagaaacacaacttttaagGCCCAGTCCGCCAAGGCCGTCGCCCCGCCCATCGCCCAGGTCACCCAGTCTTTGTGGGCCGTGCTCACTCAGTCGTTGCGGTCTCTCAcgatcgCGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCAfeIBmtINheIE1LH2LD4QT6PS8RQ10PI12PS14EG16LQ18997-2316N1S2G3S4G5G6S7G8S9G10A11G12S13G14S15S16G17S18E19T20P21G22T23S24E25S26A27T28P29E30S31A32S33A34895-996V17A18L19C20V21829-891GS+XTEN linkerhADAR2 cat domainfrom XG 205CD28-TMD8809009209409609801,0001,0201,040
    CTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAAGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTBseRIBstEIILH20LP22QV24LA26DA28VS30RL32VL34GK36FG38DL40TD42NF44SS46PH48AR50RK52VL54AG56VV58MT60TG62TD64VK66DA68KV70IS72VS74TG76997-2316hADAR2 cat domain1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGccATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCggTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCBclITK78CI80NG82EY84MS86DR88GL90AL92ND94CH96AA98II100SR102RS104LL106RF108LY110TQ112LE114LY116LN118NK120DD122QK124RS126IF128QK130SE132RG134G997-2316hADAR2 cat domain1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTgAGGGGACGATTCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAcTCCCCTGCTAABcgIPfoIBsrGIF136RL138KE140NV142QF144HL146YI148ST150SP152CG154DA156RI158FS160PH162EP164IL166EE168PA170DR172HP174NR176KA178RG180QL182RT184KI186ES188GE190GT192I997-2316hADAR2 cat domain1,4201,4401,4601,4801,5001,5201,5401,560
    CCAGTGCGCTCCAATGCGAGCATCCAAACGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCGGTCACGCGAGGTTACGCTCGTAGGTTTGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAvaIBlpIPstITliIXhoIBamHIBsoBIPaeR7IP194VR196SN198AS200IQ202TW204DG206VL208QG210ER212LL214TM216SC218SD220KI222AR224WN226VV228GI230QG232SL234LS236IF238VE240PI242YF244SS246II248LG250S997-2316hADAR2 cat domainDim...ce1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGL252YH254GD256HL258SR260AM262YQ264RI266SN268IE270DL272PP274LY276TL278NK280PL282LS284GI286SN288AE290AR292QP294GK296AP298NF300SV302NW304TV306GD308SA310997-2316hADAR2 cat domain1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCMluIIE312VI314NA316TT318GK320DE322LG324RA326SR328LC330KH332AL334YC336RW338MR340VH342GK344VP346SH348LL350RS352KI354TK356PN358VY360HE362SK364LA366AK368997-2316hADAR2 cat domain1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCCTTCTCGAAGCCCTGACCTTAGCCAGTGCACGGGGACCCCTGAGGAAGAGATCTGTGCCAATGGCTAAGGCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGGAAGAGCTTCGGGACTGGAATCGGTCACGTGCCCCTGGGGACTCCTTCTCTAGACACGGTTACCGATTCCKflIBsu36IEY370QA372AK374AR376LF378TA380FI382KA384GL386GA388WV390EK392PT394EQ396DQ398FS400LT402PL404LE406AL408TL410AS412AR414GP416LR418KR420SV422PM424AK426997-2316hADAR2 cat domainC-terminal tail of Kir6.22,1202,1402,1602,1802,2002,2202,2402,260
    CGAAACCGAAATTCTCCATCTCACCTGACAGCTTGAGCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatcGCTTTGGCTTTAAGAGGTAGAGTGGACTGTCGAACTCGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagApaIAvaIBclIBlpIPmeITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMI*1AK428PK430FS432IS434PD436SL438S*440997-2316C-terminal tail of Kir6.2c-myc epitopeBGH polyadeny...ion sequencePolyhi...e tag2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtaBGH polyadenylation sequence2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacaccgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgClaINspISphIBspDIBGH pA2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcKflINaeINgoMIV2,8202,8402,8602,8802,9002,9202,9402,960
    ttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcBarIMscIXbaIBsmBIEsp3IPshAIKK2PE4LT6AT8SV10EK12FL14IE16KF18DS20VS22DL24MQ26LS28EG30EE32SR34AF36SF38DV40G3027-4047HygromycinFRT2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgtEcoRIDraIIIG42RG44YV46LR48VN50SC52AD54GF56YK58DR60YV62YR64HF66AS68AA70LP72IP74EV76LD78IG80EF82SE84SL86TY88CI90SR92RA94QG96VT98LQ1003027-4047Hygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    agacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacNdeIPstIPvuIAsiSIRsrIIBstAPIDL102PE104TE106LP108AV110LQ112PV114AE116AM118DA120IA122AA124DL126SQ128TS130GF132GP134FG136PQ138GI140GQ142YT144TW146RD148FI150CA152IA154DP156HV1583027-4047Hygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggaatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctBstAPIDraIIINmeAIIIYH160WQ162TV164MD166DT168VS170AS172VA174QA176LD178EL180ML182WA184ED186CP188EV190RH192LV194HA196DF198GS200NN202VL204TD206NG208RI210TA212VI214DW2163027-4047Hygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    gcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcacgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtAjuISacIISE218AM220FG222DS224QY226EV228AN230IF232FW234RP236WL238AC240ME242QQ244TR246YF248ER250RH252PE254LA256GS258PR260LR262AY264ML266RI268GL270DQ272LY274Q3027-4047Hygromycin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgScaIS276LV278DG280NF282DD284AA286WA288QG290RC292DA294IV296RS298GA300GT302VG304RT306QI308AR310RS312AA314VW316TD318GC320VE322VL324AD326SG328NR330RP332S3027-4047Hygromycin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    actcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttatatgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatatNaeIPfoIPsiINgoMIV*1T334RP336RA338KE3403027-4047HygromycinSV40 pA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    atggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgtaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacBsmIBstZ17ISV40 pA4,2204,2404,2604,2804,3004,3204,3404,360
    ttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccg4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggNspIPciISapIBspQIpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggccpUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtatatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccatpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    agacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtatctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatAlwNIpUC ori5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaacgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttpUC ori5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgaAhdIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    acgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatBsaINmeAIIIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    gagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggAclIFspIAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcactagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagtBcgIPvuIScaIAmp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    atacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccaAclIXmnIAmp (bla)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgctcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcSspIbla promoterAmp (bla)6,3206,3406,3606,3806,4006,4206,4406,460
    cacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaagtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttAlwNISgrDI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWI6,6606,6806,7006,720
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6732 bp50005500600050010001500hADAR2 cat domainDimerization sequenceCMVTetR binding sitesTetO997-2316pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA)EK_CMVFa (DNA),EK_CMVFb (DNA),EK_CMVFc (DNA),EKo272.1 (DNA),SpeI,NdeICMV promoter (with TetOs),A,EK_CMVFd (DNA),EK_CMVFe (DNA),SnaBI,CspCITATA box,TetO,CMV-Forward Genewiz (DNA),Eco53kI,SacI,BsaBI,BsaBICMVF1 (DNA),ToFlpF (DNA),XZp025.1 (DNA),SgrDI,Eco53kI,SacI,BsmBI,+2Kozak,CD8α signal peptide,760-822,823-828,EKbF (DNA),+9CD28-TMD,from XG 205,829-891,892-894,XZp017.2 (DNA),+1f-cXTEN-AdarDD (DNA)GS+XTEN linker,895-996,r-cXTEN-adar (DNA),XZp017.4 (DNA),+4BstEIIBseRIE396A,YX001.2 (DNA),YX001.3 (DNA)BclIBsrGIPfoIE488Q,YX001.4 (DNA),YX001.5 (DNA),BcgIPstIpUC oriAlwNIAhdIBsaINmeAIIIFspI,AclIAmp (bla),PvuIScaI,BcgIAclI,XmnIbla promoter,SspIFlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo152.4 (DNA),EKo279.1 (DNA),SgrDI,+4

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    6732

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