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pMS79 (Cas9 with synthetic gRNA5- Targets PX696 SLP) (Github)
  • Sequence Map
    Editing disabled on read-only sequence.
    ctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgaggacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactcColE1 Origin20406080100120140160
    atcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccactttaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaaAcuIColE1 Origin180200220240260280300320340
    caagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgctAlwNIColE1 Origin360380400420440460480500520
    acgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcctgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggColE1 Origin540560580600620640660680700
    acctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggtcttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccagaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcacPciIAflIIIColE1 Origin720740760780800820840860
    agctgataccagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagctatcaactttgtatagaaaagttgGCACCTTTGGTCTTTTATTGTCAACTTCCATTGGTTCTTCCATTGTTTCTGTTAAATTAATGAATTTTTCATAAAATAAAGACATTATAtcgactatggtcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgatagttgaaacatatcttttcaacCGTGGAAACCAGAAAATAACAGTTGAAGGTAACCAAGAAGGTAACAAAGACAATTTAATTACTTAAAAAGTATTTTATTTCTGTAATATAseIpEFT-3 promoterM13 re...rimer8809009209409609801,0001,0201,040
    CAATATAAAAATGAAGAATTTATTGAAAATAAACTGCCAGAGAGAAAAAGTATGCAACACTCCCGCCGAGAGTGTTTGAAATGGTGTACGGTACATTTTCGTGCTAGGAGTTAGATGTGCAGGCAGCAACGAGAGGGGGAGAGATTTTTTTGGGCCTTGTGAAATTAACGTGAGTGTTATATTTTTACTTCTTAAATAACTTTTATTTGACGGTCTCTCTTTTTCATACGTTGTGAGGGCGGCTCTCACAAACTTTACCACATGCCATGTAAAAGCACGATCCTCAATCTACACGTCCGTCGTTGCTCTCCCCCTCTCTAAAAAAACCCGGAACACTTTAATTGCACTCABsgIpEFT-3 promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TTTCTGGTCATCTGACTAATCATGTTGGTTTTTTGTTGGTTTATTTTGTTTTTATCTTTGTTTTTATCCAGATTAGGAAATTTAAATTTTATGAATTTATAATGAGGTCAAACATTCAGTCCCAGCGTTTTTCCTGTTCTCACTGTTTAGTCGAATTTTTATTTTAGGCTTTCAAAAAGACCAGTAGACTGATTAGTACAACCAAAAAACAACCAAATAAAACAAAAATAGAAACAAAAATAGGTCTAATCCTTTAAATTTAAAATACTTAAATATTACTCCAGTTTGTAAGTCAGGGTCGCAAAAAGGACAAGAGTGACAAATCAGCTTAAAAATAAAATCCGAAAGTTPsiIpEFT-3 promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CAAATGTTCTAACTGTCTTATTTGTGACCTCACTTTTTATATTTTTTTAATTTTTAAAAATATTAGAAGTTTCTAGGATAATTTTTTCGACTTTTATTCTCTCTACCGTCCGCACTCTTCTTACTTTTAAATTAAATTGTTTTTTTTTCAGTTGGGAAACACTTTGCTCTAGAaaGTTTACAAGATTGACAGAATAAACACTGGAGTGAAAAATATAAAAAAATTAAAAATTTTTATAATCTTCAAAGATCCTATTAAAAAAGCTGAAAATAAGAGAGATGGCAGGCGTGAGAAGAATGAAAATTTAATTTAACAAAAAAAAAGTCAACCCTTTGTGAAACGAGATCTttXbaIpEFT-3 promoter1,4201,4401,4601,4801,5001,5201,5401,560
    aaATGGACAAAAAATACAGCATCGGCCTTGACATCGGAACGAACTCCGTCGGATGGGCCGTCATCACCGACGAGTACAAGGTCCCATCCAAGAAGTTCAAGGTCCTCGGAAACACCGACCGTCACTCCATCAAGAAGAACCTCATCGGAGCCCTCCTCTTCGACTCCGGAGAGACttTACCTGTTTTTTATGTCGTAGCCGGAACTGTAGCCTTGCTTGAGGCAGCCTACCCGGCAGTAGTGGCTGCTCATGTTCCAGGGTAGGTTCTTCAAGTTCCAGGAGCCTTTGTGGCTGGCAGTGAGGTAGTTCTTCTTGGAGTAGCCTCGGGAGGAGAAGCTGAGGCCTCTCTGMD2KK4YS6IG8LD10IG12TN14SV16GW18AV20IT22DE24YK26VP28SK30KF32KV34LG36NT38DR40HS42IK44KN46LI48GA50LL52FD54SG56ET581578-5903Cas9 artificial gene1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CGCCGAGGCCACCCGTCTCAAGCGTACCGCCCGTCGTCGTTACACCCGTCGTAAGAACCGTATCTGCTACCTCCAAGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCCTTCTTCCACCGTCTCGAGGAGTCCTTCCTCGTCGAGGAGGACAAGAAGCACGAGCGTCACGCGGCTCCGGTGGGCAGAGTTCGCATGGCGGGCAGCAGCAATGTGGGCAGCATTCTTGGCATAGACGATGGAGGTTCTCTAGAAGAGGTTGCTCTACCGGTTCCAGCTGCTGAGGAAGAAGGTGGCAGAGCTCCTCAGGAAGGAGCAGCTCCTCCTGTTCTTCGTGCTCGCAGTGMscIBstXIAE60AT62RL64KR66TA68RR70RY72TR74RK76NR78IC80YL82QE84IF86SN88EM90AK92VD94DS96FF98HR100LE102ES104FL106VE108ED110KK112HE114RH1161578-5903Cas9 artificial gene1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CCAATCTTCGGAAACATCGTCGACGAGGTCGCCTACCACGAGAAGTACCCAACCATCTACCACCTCCGTAAGAAGCTCGTCGACTCCACCGACAAGGCCGACCTCCGTCTCATCTACCTCGCCCTCGCCCACATGATCAAGTTCCGTGGACACTTCCTCATCGAGGGAGACCTCAGGTTAGAAGCCTTTGTAGCAGCTGCTCCAGCGGATGGTGCTCTTCATGGGTTGGTAGATGGTGGAGGCATTCTTCGAGCAGCTGAGGTGGCTGTTCCGGCTGGAGGCAGAGTAGATGGAGCGGGAGCGGGTGTACTAGTTCAAGGCACCTGTGAAGGAGTAGCTCCCTCTGGAGTBclICspCIPshAISgrDIPI118FG120NI122VD124EV126AY128HE130KY132PT134IY136HL138RK140KL142VD144ST146DK148AD150LR152LI154YL156AL158AH160MI162KF164RG166HF168LI170EG172DL1741578-5903Cas9 artificial gene1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACCCAGACAACTCCGACGTCGACAAGCTCTTCATCCAACTCGTCCAAACCTACAACCAACTCTTCGAGGAGAACCCAATCAACGCCTCCGGAGTCGACGCCAAGGCCATCCTCTCCGCCCGTCTCTCCAAGTCCCGTCGTCTCGAGAACCTCATCGCCCAACTCCCAGGAGAGAATGGGTCTGTTGAGGCTGCAGCTGTTCGAGAAGTAGGTTGAGCAGGTTTGGATGTTGGTTGAGAAGCTCCTCTTGGGTTAGTTGCGGAGGCCTCAGCTGCGGTTCCGGTAGGAGAGGCGGGCAGAGAGGTTCAGGGCAGCAGAGCTCTTGGAGTAGCGGGTTGAGGGTCCTCTCTTSapIBspQINP176DN178SD180VD182KL184FI186QL188VQ190TY192NQ194LF196EE198NP200IN202AS204GV206DA208KA210IL212SA214RL216SK218SR220RL222EN224LI226AQ228LP230GE232K1578-5903Cas9 artificial gene2,1202,1402,1602,1802,2002,2202,2402,260
    GAAGAACGGACTCTTCGGAAACCTCATCGCCCTCTCCCTCGGACTCACCCCAAACTTCAAGTCCAACTTCGACCTCGCCGAGGACGCCAAGCTCCAACTCTCCAAGGACACCTACGACGACGACCTCGACAACCTCCTCGCCCAAATCGGAGACCAATACGCCGACCTCTTCCTCCTTCTTGCCTGAGAAGCCTTTGGAGTAGCGGGAGAGGGAGCCTGAGTGGGGTTTGAAGTTCAGGTTGAAGCTGGAGCGGCTCCTGCGGTTCGAGGTTGAGAGGTTCCTGTGGATGCTGCTGCTGGAGCTGTTGGAGGAGCGGGTTTAGCCTCTGGTTATGCGGCTGGAGAAGGAGK234NG236LF238GN240LI242AL244SL246GL248TP250NF252KS254NF256DL258AE260DA262KL264QL266SK268DT270YD272DD274LD276NL278LA280QI282GD284QY286AD288LF290L1578-5903Cas9 artificial gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    GCCGCCAAGAACCTCTCCGACGCCATCCTCCTCTCCGACATCCTCCGTGTCAACACCGAGATCACCAAGGCCCCACTCTCCGCCTCCATGATCAAGCGTTACGACGAGCACCACCAAGACCTCACCCTCCTCAAGGCCCTCGTCCGTCAACAACTCCCAGAGAAGTACAAGgtaaCGGCGGTTCTTGGAGAGGCTGCGGTAGGAGGAGAGGCTGTAGGAGGCACAGTTGTGGCTCTAGTGGTTCCGGGGTGAGAGGCGGAGGTACTAGTTCGCAATGCTGCTCGTGGTGGTTCTGGAGTGGGAGGAGTTCCGGGAGCAGGCAGTTGTTGAGGGTCTCTTCATGTTCcattBclIA292AK294NL296SD298AI300LL302SD304IL306RV308NT310EI312TK314AP316LS318AS320MI322KR324YD326EH328HQ330DL332TL334LK336AL338VR340QQ342LP344EK346YK348V1578-5903Cas9 artificial gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gtttaaacatatatatactaactaaccctgattatttaaattttcagGAGATCTTCTTCGACCAATCCAAGAACGGATACGCCGGATACATCGACGGAGGAGCCTCCCAAGAGGAGTTCTACAAGTTCATCAAGCCAATCCTCGAGAAGATGGACGGAACCGAGGAGCTCCTCGTcaaatttgtatatatatgattgattgggactaataaatttaaaagtcCTCTAGAAGAAGCTGGTTAGGTTCTTGCCTATGCGGCCTATGTAGCTGCCTCCTCGGAGGGTTCTCCTCAAGATGTTCAAGTAGTTCGGTTAGGAGCTCTTCTACCTGCCTTGGCTCCTCGAGGAGCAS350LN352IY354IL356TN358PD360YL362NF364QE366IF368FD370QS372KN374GY376AG378YI380DG382GA384SQ386EE388FY390KF392IK394PI396LE398KM400DG402TE404EL406LV4081578-5903Cas9 artificial geneSynthetic intron2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CAAGCTCAACCGTGAGGACCTCCTCCGTAAGCAACGTACCTTCGACAACGGATCCATCCCACACCAAATCCACCTCGGAGAGCTCCACGCCATCCTCCGTCGTCAAGAGGACTTCTACCCATTCCTCAAGGACAACCGTGAGAAGATCGAGAAGATCCTCACCTTCCGTATCCCAGTTCGAGTTGGCACTCCTGGAGGAGGCATTCGTTGCATGGAAGCTGTTGCCTAGGTAGGGTGTGGTTTAGGTGGAGCCTCTCGAGGTGCGGTAGGAGGCAGCAGTTCTCCTGAAGATGGGTAAGGAGTTCCTGTTGGCACTCTTCTAGCTCTTCTAGGAGTGGAAGGCATAGGGTKL410NR412ED414LL416RK418QR420TF422DN424GS426IP428HQ430IH432LG434EL436HA438IL440RR442QE444DF446YP448FL450KD452NR454EK456IE458KI460LT462FR464IP4661578-5903Cas9 artificial gene2,8202,8402,8602,8802,9002,9202,9402,960
    TACTACGTCGGACCACTCGCCCGTGGAAACTCCCGTTTCGCCTGGATGACCCGTAAGTCCGAGGAGACCATCACCCCATGGAACTTCGAGGAGGTCGTCGACAAGGGAGCCTCCGCCCAATCCTTCATCGAGCGTATGACCAACTTCGACAAGAACCTCCCAAACGAGAAGGTCCATGATGCAGCCTGGTGAGCGGGCACCTTTGAGGGCAAAGCGGACCTACTGGGCATTCAGGCTCCTCTGGTAGTGGGGTACCTTGAAGCTCCTCCAGCAGCTGTTCCCTCGGAGGCGGGTTAGGAAGTAGCTCGCATACTGGTTGAAGCTGTTCTTGGAGGGTTTGCTCTTCCAGGNcoIYY468VG470PL472AR474GN476SR478FA480WM482TR484KS486EE488TI490TP492WN494FE496EV498VD500KG502AS504AQ506SF508IE510RM512TN514FD516KN518LP520NE522KV5241578-5903Cas9 artificial gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TCCCAAAGCACTCCCTCCTCTACGAGTACTTCACCGTCTACAACGAGCTCACCAAGGTCAAGTACGTCACCGAGGGAATGCGTAAGCCAGCCTTCCTCTCCGGAGAGCAAAAGAAGGCCATCGTCGACCTCCTCTTCAAGACCAACCGTAAGGTCACCGTCAAGCAACTCAAGGAAGGGTTTCGTGAGGGAGGAGATGCTCATGAAGTGGCAGATGTTGCTCGAGTGGTTCCAGTTCATGCAGTGGCTCCCTTACGCATTCGGTCGGAAGGAGAGGCCTCTCGTTTTCTTCCGGTAGCAGCTGGAGGAGAAGTTCTGGTTGGCATTCCAGTGGCAGTTCGTTGAGTTCCTBstEIILP526KH528SL530LY532EY534FT536VY538NE540LT542KV544KY546VT548EG550MR552KP554AF556LS558GE560QK562KA564IV566DL568LF570KT572NR574KV576TV578KQ580LK582E1578-5903Cas9 artificial gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCCGGAGTCGAGGACCGTTTCAACGCCTCCCTCGGAACCTACCACGACCTCCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTCGAGGACATCGTCCTCACCCTCACCCCTGATGAAGTTCTTCTAGCTCACGAAGCTGAGGCAGCTCTAGAGGCCTCAGCTCCTGGCAAAGTTGCGGAGGGAGCCTTGGATGGTGCTGGAGGAGTTCTAGTAGTTCCTGTTCCTGAAGGAGCTGTTGCTCCTCTTGCTCCTGTAGGAGCTCCTGTAGCAGGAGTGGGAGTGGD584YF586KK588IE590CF592DS594VE596IS598GV600ED602RF604NA606SL608GT610YH612DL614LK616II618KD620KD622FL624DN626EE628NE630DI632LE634DI636VL638TL640T1578-5903Cas9 artificial gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CTCTTCGAGGACCGTGAGATGATCGAGGAGCGTCTCAAGACCTACGCCCACCTCTTCGACGACAAGGTCATGAAGCAgCTtAAGCGTCGTCGTTACACCGGATGGGGACGTCTCTCCCGTAAGCTCATCAACGGAATCCGTGACAAGCAATCCGGAAAGACCATCCTCGACTTCCGAGAAGCTCCTGGCACTCTACTAGCTCCTCGCAGAGTTCTGGATGCGGGTGGAGAAGCTGCTGTTCCAGTACTTCGTcGAaTTCGCAGCAGCAATGTGGCCTACCCCTGCAGAGAGGGCATTCGAGTAGTTGCCTTAGGCACTGTTCGTTAGGCCTTTCTGGTAGGAGCTGAAGGAflIIL642FE644DR646EM648IE650ER652LK654TY656AH658LF660DD662KV664MK666QL668KR670RR672YT674GW676GR678LS680RK682LI684NG686IR688DK690QS692GK694TI696LD698F1578-5903Cas9 artificial gene3,5203,5403,5603,5803,6003,6203,6403,660
    TCAAGTCCGACGGATTCGCCAACCGTAACTTCATGCAACTCATCCACGACGACTCCCTCACCTTCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGAGGACATCCAAAAGGCCCAAGTCTCCGGACAAGGAGACTCCCTCCACGAGCACATAGTTCAGGCTGCCTAAGCGGTTGGCATTGAAGTACGTTGAGTAGGTGCTGCTGAGGGAGTGGAAGTTCcattcaaatttgtcaagccatgattgattggtatgtataaatttaaaagtcCTCCTGTAGGTTTTCCGGGTTCAGAGGCCTGTTCCTCTGAGGGAGGTGCTCGTGTAAjuIL700KS702DG704FA706NR708NF710MQ712LI714HD716DS718LT720FK722VS724LN726SS728VL730TN732HT734YL736NF738QE740DI742QK744AQ746VS748GQ750GD752SL754HE756HI7581578-5903Cas9 artificial geneSynthetic intron3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CGCCAACCTCGCCGGATCCCCAGCCATCAAGAAGGGAATCCTCCAAACCGTCAAGGTCGTCGACGAGCTCGTCAAGGTCATGGGACGTCACAAGCCAGAGAACATCGTCATCGAGATGGCCCGTGAGAACCAAACCACCCAAAAGGGACAAAAGAACTCCCGTGAGCGTATGAAGGCGGTTGGAGCGGCCTAGGGGTCGGTAGTTCTTCCCTTAGGAGGTTTGGCAGTTCCAGCAGCTGCTCGAGCAGTTCCAGTACCCTGCAGTGTTCGGTCTCTTGTAGCAGTAGCTCTACCGGGCACTCTTGGTTTGGTGGGTTTTCCCTGTTTTCTTGAGGGCACTCGCATACTTCSgrDIAN760LA762GS764PA766IK768KG770IL772QT774VK776VV778DE780LV782KV784MG786RH788KP790EN792IV794IE796MA798RE800NQ802TT804QK806GQ808KN810SR812ER814MK8161578-5903Cas9 artificial gene3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CGTATCGAGGAGGGAATCAAGGAGCTCGGATCCCAAATCCTCAAGGAGCACCCAGTCGAGAACACCCAACTCCAAAACGAGAAGCTCTACCTCTACTACCTCCAAAACGGACGTGACATGTACGTCGACCAAGAGCTCGACATCAACCGTCTCTCCGACTACGACGTCGACCACAGCATAGCTCCTCCCTTAGTTCCTCGAGCCTAGGGTTTAGGAGTTCCTCGTGGGTCAGCTCTTGTGGGTTGAGGTTTTGCTCTTCGAGATGGAGATGATGGAGGTTTTGCCTGCACTGTACATGCAGCTGGTTCTCGAGCTGTAGTTGGCAGAGAGGCTGATGCTGCAGCTGGTGTPciIAflIIIRI818EE820GI822KE824LG826SQ828IL830KE832HP834VE836NT838QL840QN842EK844LY846LY848YL850QN852GR854DM856YV858DQ860EL862DI864NR866LS868DY870DV872DH8741578-5903Cas9 artificial gene4,0404,0604,0804,1004,1204,1404,1604,1804,200
    TCGTCCCACAATCCTTCCTCAAGGACGACTCCATCGACAACAAGGTCCTCACCCGTTCCGACAAGAACCGTGGAAAGTCCGACAACGTCCCATCCGAGGAGGTCGTCAAGAAGATGAAGAACTACTGGCGTCAACTCCTCAACGCCAAGCTCATCACCCAACGTAAGTTCGACAAAGCAGGGTGTTAGGAAGGAGTTCCTGCTGAGGTAGCTGTTGTTCCAGGAGTGGGCAAGGCTGTTCTTGGCACCTTTCAGGCTGTTGCAGGGTAGGCTCCTCCAGCAGTTCTTCTACTTCTTGATGACCGCAGTTGAGGAGTTGCGGTTCGAGTAGTGGGTTGCATTCAAGCTGTTCspCIIV876PQ878SF880LK882DD884SI886DN888KV890LT892RS894DK896NR898GK900SD902NV904PS906EE908VV910KK912MK914NY916WR918QL920LN922AK924LI926TQ928RK930FD932N1578-5903Cas9 artificial gene4,2204,2404,2604,2804,3004,3204,3404,360
    CCTCACCAAGGCTGAGCGTGGAGGACTCTCCGAGCTCGACAAGGCCGGATTCATCAAGCGTCAACTCGTCGAGACCCGTCAAATCACCAAGCACGTCGCCCAAATCCTCGACTCCCGTATGAACACCAAGTACGACGAGAACGACAAGCTCATCCGTGAGGTCAAGGTCATCACCGGAGTGGTTCCGACTCGCACCTCCTGAGAGGCTCGAGCTGTTCCGGCCTAAGTAGTTCGCAGTTGAGCAGCTCTGGGCAGTTTAGTGGTTCGTGCAGCGGGTTTAGGAGCTGAGGGCATACTTGTGGTTCATGCTGCTCTTGCTGTTCGAGTAGGCACTCCAGTTCCAGTAGTGGBlpIPsrIL934TK936AE938RG940GL942SE944LD946KA948GF950IK952RQ954LV956ET958RQ960IT962KH964VA966QI968LD970SR972MN974TK976YD978EN980DK982LI984RE986VK988VI990T1578-5903Cas9 artificial gene4,3804,4004,4204,4404,4604,4804,5004,5204,540
    CTCAAGTCCAAGCTCGTCTCCGACTTCCGTAAGGACTTCCAATTCTACAAGGTCCGTGAGATCAACAACTACCACCACGCCCACGACGCCTACCTCAACGCCGTAGTAGGAACCGCCCTCATCAAGAAGTACCCAAAGCTCGAGTCCGAGTTCGTCTACGGAGACTACAAGGTCTGAGTTCAGGTTCGAGCAGAGGCTGAAGGCATTCCTGAAGGTTAAGATGTTCCAGGCACTCTAGTTGTTGATGGTGGTGCGGGTGCTGCGGATGGAGTTGCGGCATCATCCTTGGCGGGAGTAGTTCTTCATGGGTTTCGAGCTCAGGCTCAAGCAGATGCCTCTGATGTTCCAGAL992KS994KL996VS998DF1000RK1002DF1004QF1006YK1008VR1010EI1012NN1014YH1016HA1018HD1020AY1022LN1024AV1026VG1028TA1030LI1032KK1034YP1036KL1038ES1040EF1042VY1044GD1046YK1048V1578-5903Cas9 artificial gene4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ACGACGTCCGTAAGATGATCGCCAAGTCCGAGCAAGAGATCGGAAAGGCCACCGCCAAGTACTTCTTCTACTCCAACATCATGAACTTCTTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCGAGATCACCCTCGCCAACGGAGAGATTGCTGCAGGCATTCTACTAGCGGTTCAGGCTCGTTCTCTAGCCTTTCCGGTGGCGGTTCATGAAGAAGATGAGGTTGTAGTACTTGAAGAAGTTCcattcaaatttgtactaaaatgattgattgattagactaaatttaaaagtcTGGCTCTAGTGGGAGCGGTTGCCTCTCTAAjuIXmnIY1050DV1052RK1054MI1056AK1058SE1060QE1062IG1064KA1066TA1068KY1070FF1072YS1074NI1076MN1078FF1080KV1082SL1084NM1086IL1088LT1090N*1092SD1094LN1096FQ1098TE1100IT1102LA1104NG1106EI11081578-5903Cas9 artificial geneSynthetic intron4,7404,7604,7804,8004,8204,8404,8604,8804,900
    CCGTAAGCGTCCACTCATCGAGACCAACGGAGAGACCGGAGAGATCGTCTGGGACAAGGGACGTGACTTCGCCACAGTCCGTAAGGTCCTCTCCATGCCACAAGTCAACATCGTCAAGAAGACCGAGGTCCAAACCGGAGGATTCTCCAAGGAGTCCATCCTCCCAAAGCGTAACGGCATTCGCAGGTGAGTAGCTCTGGTTGCCTCTCTGGCCTCTCTAGCAGACCCTGTTCCCTGCACTGAAGCGGTGTCAGGCATTCCAGGAGAGGTACGGTGTTCAGTTGTAGCAGTTCTTCTGGCTCCAGGTTTGGCCTCCTAAGAGGTTCCTCAGGTAGGAGGGTTTCGCATTGBbsIPshAIRK1110RP1112LI1114ET1116NG1118ET1120GE1122IV1124WD1126KG1128RD1130FA1132TV1134RK1136VL1138SM1140PQ1142VN1144IV1146KK1148TE1150VQ1152TG1154GF1156SK1158ES1160IL1162PK1164RN11661578-5903Cas9 artificial gene4,9204,9404,9604,9805,0005,0205,0405,060
    TCCGACAAGCTCATCGCCCGTAAGAAGGACTGGGACCCAAAGAAGTACGGAGGATTCGACTCCCCAACCGTCGCCTACTCCGTCCTCGTCGTCGCCAAGGTCGAGAAGGGAAAGTCCAAGAAGCTCAAGTCCGTCAAGGAGCTCCTCGGAATCACCATCATGGAGCGTTCCTCCTAGGCTGTTCGAGTAGCGGGCATTCTTCCTGACCCTGGGTTTCTTCATGCCTCCTAAGCTGAGGGGTTGGCAGCGGATGAGGCAGGAGCAGCAGCGGTTCCAGCTCTTCCCTTTCAGGTTCTTCGAGTTCAGGCAGTTCCTCGAGGAGCCTTAGTGGTAGTACCTCGCAAGGAGGAKflISD1168KL1170IA1172RK1174KD1176WD1178PK1180KY1182GG1184FD1186SP1188TV1190AY1192SV1194LV1196VA1198KV1200EK1202GK1204SK1206KL1208KS1210VK1212EL1214LG1216IT1218IM1220ER1222SS12241578-5903Cas9 artificial gene5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TCGAGAAGAACCCAATCGACTTCCTCGAGGCCAAGGGATACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTCCCAAAGTACTCCCTCTTCGAGCTCGAGAACGGACGTAAGCGTATGCTCGCCTCCGCCGGAGAGCTCCAAAAGGGAAACGAGCTCGCCCTCCCATCCAAGTAAGCTCTTCTTGGGTTAGCTGAAGGAGCTCCGGTTCCCTATGTTCCTCCAGTTCTTCCTGGAGTAGTAGTTCGAGGGTTTCATGAGGGAGAAGCTCGAGCTCTTGCCTGCATTCGCATACGAGCGGAGGCGGCCTCTCGAGGTTTTCCCTTTGCTCGAGCGGGAGGGTAGGTTCATBarIFE1226KN1228PI1230DF1232LE1234AK1236GY1238KE1240VK1242KD1244LI1246IK1248LP1250KY1252SL1254FE1256LE1258NG1260RK1262RM1264LA1266SA1268GE1270LQ1272KG1274NE1276LA1278LP1280SK1282Y1578-5903Cas9 artificial gene5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CGTCAACTTCCTCTACCTCGCCTCCCACTACGAGAAGCTCAAGGGATCCCCAGAGGACAACGAGCAAAAGCAACTCTTCGTCGAGCAACACAAGCACTACCTCGACGAGATCATCGAGCAAATCTCCGAGTTCTCCAAGCGTGTCATCCTCGCCGACGCCAACCTCGACAAGGTCGCAGTTGAAGGAGATGGAGCGGAGGGTGATGCTCTTCGAGTTCCCTAGGGGTCTCCTGTTGCTCGTTTTCGTTGAGAAGCAGCTCGTTGTGTTCGTGATGGAGCTGCTCTAGTAGCTCGTTTAGAGGCTCAAGAGGTTCGCACAGTAGGAGCGGCTGCGGTTGGAGCTGTTCCAGBarIV1284NF1286LY1288LA1290SH1292YE1294KL1296KG1298SP1300ED1302NE1304QK1306QL1308FV1310EQ1312HK1314HY1316LD1318EI1320IE1322QI1324SE1326FS1328KR1330VI1332LA1334DA1336NL1338DK1340V1578-5903Cas9 artificial gene5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CTCTCCGCCTACAACAAGCACCGTGACAAGCCAATCCGTGAGCAAGCCGAGAACATCATCCACCTCTTCACCCTCACCAACCTCGGAGCCCCAGCCGCCTTCAAGTACTTCGACACCACCATCGACCGTAAGCGTTACACCTCCACCAAGGAGGTCCTCGACGCCACCCTCATCCGAGAGGCGGATGTTGTTCGTGGCACTGTTCGGTTAGGCACTCGTTCGGCTCTTGTAGTAGGTGGAGAAGTGGGAGTGGTTGGAGCCTCGGGGTCGGCGGAAGTTCATGAAGCTGTGGTGGTAGCTGGCATTCGCAATGTGGAGGTGGTTCCTCCAGGAGCTGCGGTGGGAGTAGGL1342SA1344YN1346KH1348RD1350KP1352IR1354EQ1356AE1358NI1360IH1362LF1364TL1366TN1368LG1370AP1372AA1374FK1376YF1378DT1380TI1382DR1384KR1386YT1388ST1390KE1392VL1394DA1396TL1398I1578-5903Cas9 artificial gene5,6205,6405,6605,6805,7005,7205,7405,760
    ACCAATCCATCACCGGACTCTACGAGACCCGTATCGACCTCTCCCAACTCGGAGGAGACGGAGGATCCGGACCACCAAAGAAGAAGCGTAAGGTCTACCCATACGACGTCCCAGACTACGCCTAAACTAGTgataaatgcaaaatcctttcaagcattcccttcttctctatcacTGGTTAGGTAGTGGCCTGAGATGCTCTGGGCATAGCTGGAGAGGGTTGAGCCTCCTCTGCCTCCTAGGCCTGGTGGTTTCTTCTTCGCATTCCAGATGGGTATGCTGCAGGGTCTGATGCGGATTTGATCActatttacgttttaggaaagttcgtaagggaagaagagatagtgSpeIH1400QS1402IT1404GL1406YE1408TR1410ID1412LS1414QL1416GG1418DG1420GS1422GP1424PK1426KK1428RK1430VY1432PY1434DV1436PD1438YA1440*T14421578-5903Cas9 artificial genetbb-2 3'UTRHA tagSV40 NLS5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tcttctttctttttgtcaaaaaattctctcgctaatttatttgcttttttaatgttattattttatgactttttatagtcactgaaaagtttgcatctgagtgaagtgaatgctatcaaaatgtgattctgtctgatgtactttcacaatctctcttcaattccattttgaagtgagaagaaagaaaaacagttttttaagagagcgattaaataaacgaaaaaattacaataataaaatactgaaaaatatcagtgacttttcaaacgtagactcacttcacttacgatagttttacactaagacagactacatgaaagtgttagagagaagttaaggtaaaacttcactbb-2 3'UTR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ctttaaacccgaaaggttgagaaaaatgcgagcgctcaaatatttgtattgtgttcgttgagtgacccaacaaaaagaggaaactttattgtgccgccaagaaaaaagtctcaCAACTTTattatacatagttgataattcactggccgtcgttttacaacgtcgtgactgggaagaaatttgggctttccaactctttttacgctcgcgagtttataaacataacacaagcaactcactgggttgtttttctcctttgaaataacacggcggttcttttttcagagtGTTGAAAtaatatgtatcaactattaagtgaccggcagcaaaatgttgcagcactgacccttAfeItbb-2 3'UTRM13 f...imer6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aaccaaaaaaaactagcaataaaggaataaaaaactgtacaccttaaaggcgcacactctgttttgcaaattttatttttagttgtgaattttctgctgagacctgaaaatagcaactttagtactactataatttgtcaaccttttcaaaaaaagcatgcaatttttgagaaacttggttttttttgatcgttatttccttattttttgacatgtggaatttccgcgtgtgagacaaaacgtttaaaataaaaatcaacacttaaaagacgactctggacttttatcgttgaaatcatgatgatattaaacagttggaaaagtttttttcgtacgttaaaaactctttgSphIBsrGIU6 Promoter6,3206,3406,3606,3806,4006,4206,4406,460
    tcttataaaagctattattaaaaaaacaccttttttccaaaattattccacaaaaaatatgttatgaaatgcctacaccctctcacacacactctttatactactctgtcaaactcacgagatgtctgccgcctcttgtgttgcccctatataaacacctcctattgcgagatgtagaatattttcgataataatttttttgtggaaaaaaggttttaataaggtgttttttatacaatactttacggatgtgggagagtgtgtgtgagaaatatgatgagacagtttgagtgctctacagacggcggagaacacaacggggatatatttgtggaggataacgctctacaBcgIPsiIU6 Promoter6,4806,5006,5206,5406,5606,5806,6006,6206,640
    cttGGACAGTCCTGCCGAGGTGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTGTGAAATTTctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggaaCCTGTCAGGACGGCTCCACCCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTCACCGTGGCTCAGCCACGAAAAACACTTTAAAgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttacBglIFspIPvuICRISPR SequenceU6...r6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtgcaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcacgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatKasINarINdeISfoIPluTIBstAPI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaaccgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggPfoI7,0207,0407,0607,0807,1007,1207,1407,160
    cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaaggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttBsrBIMS2IQ4HF6RV8AL10IP12FF14AA16FC18LP20VF22AH24PE267272-8132AmpR promoterAmpR ORF7,1807,2007,2207,2407,2607,2807,3007,3207,340
    cgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggccAclIAcuIXmnITL28VK30VK32DA34ED36QL38GA40RV42GY44IE46LD48LN50SG52KI54LE56SF58RP60EE62RF64PM66MS68TF70KV72LL74CG76AV78LS80RI82DA84G7272-8132AmpR ORF7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgcgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcBcgIBtsIBtsIPvuIBtsαIBtsαIQ86EQ88LG90RR92IH94YS96QN98DL100VE102YS104PV106TE108KH110LT112DG114MT116VR118EL120CS122AA124IT126MS128DN130TA132AN134LL136LT138TI140GG142P7272-8132AmpR ORF7,5407,5607,5807,6007,6207,6407,6607,6807,700
    aaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggAclIFspIBsrDIK144EL146TA148FL150HN152MG154DH156VT158RL160DR162WE164PE166LN168EA170IP172ND174ER176DT178TM180PV182AM184AT186TL188RK190LL192TG194EL196LT198LA200S7272-8132AmpR ORF7,7207,7407,7607,7807,8007,8207,8407,860
    ggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctaccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatAseIBglIBpmIBsrDIBsrFIR202QQ204LI206DW208ME210AD212KV214AG216PL218LR220SA222LP224AG226WF228IA230DK232SG234AG236ER238GS240RG242II244AA246LG248PD250GK252PS254RI256VV258IY2607272-8132AmpR ORF7,8807,9007,9207,9407,9607,9808,0008,0208,040
    cacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaagtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattAhdITT262GS264QA266TM268DE270RN272RQ274IA276EI278GA280SL282IK284HW286*7272-8132AmpR ORF8,0608,0808,1008,120
    • Plasmid
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    8132 bp60006500700075005001000150020001578-59037272-8132

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    8132