Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pIB-hbP2-evePr-MS2v5-LacZ-Tub3UTR
  • Sequence Map
    Editing disabled on read-only sequence.
    aaccccactatgtttttctttgccttttctttctcccagcgatcattcatttcgtggtgaaagaacggggtcattgcacggagtttcgactgcgggaaagcagagctgccgttcacttcgtctataattagcgctttctattttccccgattcgggccgctgctgcgcttttccgcctttggggtgatacaaaaagaaacggaaaagaaagagggtcgctagtaagtaaagcaccactttcttgccccagtaacgtgcctcaaagctgacgccctttcgtctcgacggcaagtgaagcagatattaatcgcgaaagataaaaggggctaagcccggcgacgacgcgaaaaggcggasource20406080100120140160
    gctgtttgtggcaagtgtagcagcaggctgtgcacgcaggtggcatgcccgccgtgaccgtcgagaacccgctgacgctgccccgcgtatccgcacccgccgacgccgtcgcacgtcccgtgctcaccgtgaccaccgcgcccagcggtttcgagggcgagggcttcccggtgcgccgcgacaaacaccgttcacatcgtcgtccgacacgtgcgtccaccgtacgggcggcactggcagctcttgggcgactgcgacggggcgcataggcgtgggcggctgcggcagcgtgcagggcacgagtggcactggtggcgcgggtcgccaaagctcccgctcccgaagggccacgcggcPaqCIPflFIBstAPITth111Isource180200220240260280300320340
    cgcgttcgccgggatcaactaccgccacctcgacccgttcatcatgatggaccagatgggtgaggtggagtacgcgcccggggagcccaagggcacgccctggcacccgcaccgcggcttcgagaccgacctacatcacggccgaatgaagggcgaattctgcagatatccatcacacgcgcaagcggccctagttgatggcggtggagctgggcaagtagtactacctggtctacccactccacctcatgcgcgggcccctcgggttcccgtgcgggaccgtgggcgtggcgccgaagctctggctggatgtagtgccggcttacttcccgcttaagacgtctataggtagtgtgEagIPstISmaIXmaITspMIsource360380400420440460480500520
    tggcggccgccaccgcggtggagctccagcttttgttccctttagtgagggttaatttcgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgaaccgccggcggtggcgccacctcgaggtcgaaaacaagggaaatcactcccaattaaagctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactEagINotISacIEco53kIpromoter for... lac operonpromot...erasecommo...antsThe l...TG).source540560580600620640660680700
    gctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccasource720740760780800820840860880
    atcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagtagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtcPciIhigh-copy-number ColE1/p...C origin of replicationsource9009209409609801,0001,0201,0401,060
    aggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgtccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagchigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationsource1,0801,1001,1201,1401,1601,1801,2001,2201,240
    ttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaaaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaactthigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationsource1,2601,2801,3001,3201,3401,3601,3801,4001,420
    gtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagtthigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationsource1,4401,4601,4801,5001,5201,5401,5601,5801,600
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttasource1,6201,6401,6601,6801,7001,7201,7401,7601,780
    gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcccgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggAhdIbla CDSsource1,8001,8201,8401,8601,8801,9001,9201,940
    agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgAclIbla CDSsource1,9601,9802,0002,0202,0402,0602,0802,1002,120
    tccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacbla CDSsource2,1402,1602,1802,2002,2202,2402,2602,2802,300
    tgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctagAclIScaIXmnIbla CDSsource2,3202,3402,3602,3802,4002,4202,4402,4602,480
    cagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcbla CDSbla promotersource2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccCspCIf1 bacteriophage origin of replication;...ates direction of (+) strand synthesisbla promotersource2,6802,7002,7202,7402,7602,7802,8002,8202,840
    caaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgagggttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccAloIPsiIBsaAIDraIIIf1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesissource2,8602,8802,9002,9202,9402,9602,9803,0003,020
    tgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgcacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcgNaeINgoMIVf1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesissource3,0403,0603,0803,1003,1203,1403,1603,1803,200
    cgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacommo...antsf1 b...sissource3,2203,2403,2603,2803,3003,3203,3403,3603,380
    gtaatacgactcactatagggcgaattgggtaccgggccccccctcgacgatgtaggtcacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgggctccccgggcgcgtactccacctcacccatctggtccatcatgatgaacgggtcgaggtggcggtagttgatccccattatgctgagtgatatcccgcttaacccatggcccgggggggagctgctacatccagtgccagagcttcggcgccacgcccacggtcccgcacgggaacccgaggggcccgcgcatgaggtggagtgggtagaccaggtagtactacttgcccagctccaccgccatcaactagggApaIKpnISmaIXmaITspMIAcc65IPspOMIEcoO109Ipromot...erasesource3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ggcgaacgcgcggcgcaccgggaagccctcgccctcgaaaccgctgggcgcggtggtcacggtgagcacgggacgtgcgacggcgtcggcgggtgcggatacgcggggcagcgtcagcgggttctcgacggtcacggcgggcatgtcgaggtcgacggtatcgataagcttgatatcgccgcttgcgcgccgcgtggcccttcgggagcgggagctttggcgacccgcgccaccagtgccactcgtgccctgcacgctgccgcagccgcccacgcctatgcgccccgtcgcagtcgcccaagagctgccagtgccgcccgtacagctccagctgccatagctattcgaactatagcClaIBspDIPflFIHindIIITth111Icommo...antsso...esource3,5803,6003,6203,6403,6603,6803,7003,720
    aattcgaagatctCACGCTAGCTGCCTACTCCTGCTGTCGACTCCTGACCAACGTAATCCCCATAGAAAACCGGTGGAAAATTCGCAGCTCGCTGCTAAGCTGGCCATCCGCTAAGCTCCCGGATCATCCAAATCCAAGTGCGCATAATTTTTTGTTTCTGCTCTAATCCAGAATGGAttaagcttctagaGTGCGATCGACGGATGAGGACGACAGCTGAGGACTGGTTGCATTAGGGGTATCTTTTGGCCACCTTTTAAGCGTCGAGCGACGATTCGACCGGTAGGCGATTCGAGGGCCTAGTAGGTTTAGGTTCACGCGTATTAAAAAACAAAGACGAGATTAGGTCTTACCTAgeIBmtIMscINheIPfoIBglIIFspAIHb P2 enhancersource3,7403,7603,7803,8003,8203,8403,8603,8803,900
    TCAAGAGCGCAATCCTCAATCCGCGATCCGTGATCCTCGATTCCCGACCGATCCGCGACCTGTACCTGACTTCCCGTCACCTCTGCCCATCTAATCCCTTGACGCGTGCATCCGTCTACCTGAGCGATggcgcgccaactgcagagcgcagcggtataaaagggcgcggggtggctgaAGTTCTCGCGTTAGGAGTTAGGCGCTAGGCACTAGGAGCTAAGGGCTGGCTAGGCGCTGGACATGGACTGAAGGGCAGTGGAGACGGGTAGATTAGGGAACTGCGCACGTAGGCAGATGGACTCGCTAccgcgcggttgacgtctcgcgtcgccatattttcccgcgccccaccgactAhdIAscIPstIBpu10IBssHIIHb P2 enhancereve promotersource3,9203,9403,9603,9804,0004,0204,0404,0604,080
    gagcagcacactcgagctgtgaccgccgcacagtcaacaactaactgccttcgttaatatcctctgaataagccaactttgaatcacaagacgcataccaaacatgcacggataccgaacctacaacatggagagccaccatgcccatcacgacgccagtcccgtggacctagaATGCctcgtcgtgtgagctcgacactggcggcgtgtcagttgttgattgacggaagcaattataggagacttattcggttgaaacttagtgttctgcgtatggtttgtacgtgcctatggcttggatgttgtacctctcggtggtacgggtagtgctgcggtcagggcacctggatctTACGTliIXhoIPshAIPspXIPaeR7Ieve promotersource4,1004,1204,1404,1604,1804,2004,2204,2404,260
    ATaacctacaaacgggtggaggatcaccccacccgacacttcacaatcaaggggtacaatacacaagggtggaggaacaccccaccctccagacacattacacagaaatccaatcaaacagaagcaccatcagggcttctgctaccaaatttatctcaaaaaactacaacaaggaatcTAttggatgtttgcccacctcctagtggggtgggctgtgaagtgttagttccccatgttatgtgttcccacctccttgtggggtgggaggtctgtgtaatgtgtctttaggttagtttgtcttcgtggtagtcccgaagacgatggtttaaatagagttttttgatgttgttccttagBsmINsiI24xMS2V5 misc_featuresource4,2804,3004,3204,3404,3604,3804,4004,4204,440
    accatcagggattccctgtgcaatatacgtcaaacgagggccacgacgggaggacgatcacgcctcccgaatatcggcatgtctggctttcgaattcagtgcgtggagcatcagcccacgcagccaatcagagtcgaatacaagtcgactttcgcgaagagcatcagccttcgcgccatggtagtccctaagggacacgttatatgcagtttgctcccggtgctgccctcctgctagtgcggagggcttatagccgtacagaccgaaagcttaagtcacgcacctcgtagtcgggtgcgtcggttagtctcagcttatgttcagctgaaagcgcttctcgtagtcggaagcgcggtNruI24xMS2V5 misc_featuresource4,4604,4804,5004,5204,5404,5604,5804,6004,620
    ttcttacacaaaccacactctcccctacaggaacagcatcagcgttcctgcccagtacccaactcaagaaaatttatgtccccatgcagcatcagcgcatgggccccaagaatacatccccaacaaaatcacatccgagcaccaacagggctcggagtgttgtttcttgtccaactggaagaatgtgtttggtgtgagaggggatgtccttgtcgtagtcgcaaggacgggtcatgggttgagttcttttaaatacaggggtacgtcgtagtcgcgtacccggggttcttatgtaggggttgttttagtgtaggctcgtggttgtcccgagcctcacaacaaagaacaggttgaccApaIPspOMIEcoO109I24xMS2V5 misc_featuresource4,6404,6604,6804,7004,7204,7404,7604,7804,800
    acaaaccctccatggaccatcaggccatggactctcaccaacaagacaaaaactactcttctcgaagcagcatcagcgcttcgaaacactcgagcatacattgtgcctatttcttgggtggacgatcacgccacccatgctctcacgaatttcaaaacacggacaaggacgagcaccatgtttgggaggtacctggtagtccggtacctgagagtggttgttctgtttttgatgagaagagcttcgtcgtagtcgcgaagctttgtgagctcgtatgtaacacggataaagaacccacctgctagtgcggtgggtacgagagtgcttaaagttttgtgcctgttcctgctcgtggtNcoINcoITliIXhoIPspXIPaeR7I24xMS2V5 misc_featuresource4,8204,8404,8604,8804,9004,9204,9404,9604,980
    ccagggctcgtcgttccacgtccaatacgattacttacctttcgggatcacgatcacggatcccgcagctacatcacttccactcaggacattcaagcatgcacgatcacggcatgctccacaagtctcaaccacagaaactaccaaatgggttcagcaccagcgaacccactcctacggtcccgagcagcaaggtgcaggttatgctaatgaatggaaagccctagtgctagtgcctagggcgtcgatgtagtgaaggtgagtcctgtaagttcgtacgtgctagtgccgtacgaggtgttcagagttggtgtctttgatggtttacccaagtcgtggtcgcttgggtgaggatgBamHI24xMS2V5 misc_featuresource5,0005,0205,0405,0605,0805,1005,1205,1405,160
    ctcaaacctcttcccacaaaactggcaagcaggatcaccgcttgcccattccaacataccaaatcaaaaacaattactggtacagcatcagcgtaccagcccacatctctcactactatcaaaaaccaaaccgttcagcaacagcgaacggtacacacggaaaaatcaactggtttacgagtttggagaagggtgttttgaccgttcgtcctagtggcgaacgggtaaggttgtatggtttagtttttgttaatgaccatgtcgtagtcgcatggtcgggtgtagagagtgatgatagtttttggtttggcaagtcgttgtcgcttgccatgtgtgcctttttagttgaccaaatg24xMS2V5 misc_featuresource5,1805,2005,2205,2405,2605,2805,3005,3205,340
    aaatacgaaagacgatcacgctttcgtccagcgcaaactattacgaaaaacatccgacgggaagagcaacagccttcccgcggcggaaaacctcacaaaaacacgacaaacggatgcacgaacacggcatccgccgacaacccacaaacttacaaccaggcaaacggtgcaggatcactttatgctttctgctagtgcgaaagcaggtcgcgtttgataatgctttttgtaggctgcccttctcgttgtcggaagggcgccgccttttggagtgtttttgtgctgtttgcctacgtgcttgtgccgtaggcggctgttgggtgtttgaatgttggtccgtttgccacgtcctagtgAloIBstAPI24xMS2V5 misc_featuresource5,3605,3805,4005,4205,4405,4605,4805,500
    cgcaccgtacatcaaacacctcagatctcatACTAGTgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatgcgtggcatgtagtttgtggagtctagagtaTGATCAcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaSpeIBglIIlacZ CDS24xMS2V5 misc_featuresource5,5205,5405,5605,5805,6005,6205,6405,6605,680
    ggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtaacctatcccattacggtcaatccgccgtttgttcccgcttaccgcgaaacggaccaaaggccgtggtcttcgccacggcctttcgaccgacctcacgctagaaggactccggctatgacagcagcaggggagtttgaccgtctacgtgccaatgctacgcgggtagatgtggttgcattggatagggtaatgccagttaggcggcaaacaagBaeIBsu36IlacZ CDSsource5,7005,7205,7405,7605,7805,8005,8205,8405,860
    ccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagggtgcctcttaggctgcccaacaatgagcgagtgtaaattacaactactttcgaccgatgtccttccggtctgcgcttaataaaaactaccgcaattgagccgcaaagtagacaccacgttgcccgcgacccagccaatgccggtcctgtcagcaaacggcagacttaaactggactcBpu10IlacZ CDSsource5,8805,9005,9205,9405,9605,9806,0006,0206,040
    cgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgttggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgcgtaaaaatgcgcggcctcttttggcggagcgccactaccacgacgcaacctcactgccgtcaatagaccttctagtcctatacaccgcctactcgccgtaaaaggcactgcagagcaacgacgtatttggctgatgtgtttagtcgctaaaggtacaacggtgagcgaaattactaZraIAatIIBsmBIEsp3IlacZ CDSsource6,0606,0806,1006,1206,1406,1606,1806,2006,220
    gatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtcctaaagtcggcgcgacatgacctccgacttcaagtctacacgccgctcaacgcactgatggatgcccattgtcaaagaaataccgtcccactttgcgtccagcggtcgccgtggcgcggaaagccgccactttaatagctactcgcaccaccaatacggctagcgcagtgtgatgcagClaIBspDIlacZ CDSsource6,2406,2606,2806,3006,3206,3406,3606,3806,400
    tgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgacttgcagcttttgggctttgacacctcgcggctttagggcttagagatagcacgccaccaacttgacgtgtggcggctgccgtgcgactaacttcgtcttcggacgctacagccaaaggcgctccacgcctaacttttaccagacgacgacgacttgccgttcggcaacgactaagclacZ CDSsource6,4206,4406,4606,4806,5006,5206,5406,5606,580
    aggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggattccgcaattggcagtgctcgtagtaggagacgtaccagtccagtacctactcgtctgctaccacgtcctataggacgactacttcgtcttgttgaaattgcggcacgcgacaagcgtaataggcttggtaggcgacaccatgtgcgacacgctggcgatgccggacatacaccacctaDraIIIlacZ CDSsource6,6006,6206,6406,6606,6806,7006,7206,7406,760
    gaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgccttcggttataactttgggtgccgtaccacggttacttagcagactggctactaggcgcgaccgatggccgctactcgcttgcgcattgcgcttaccacgtcgcgctagcattagtgggctcacactagtagaccagcgaccccttacttagtccggtgccgcgattagtgctgcgcgBclIPflMIlacZ CDSsource6,7806,8006,8206,8406,8606,8806,9006,9206,940
    tgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacacatagcgacctagtttagacagctaggaagggcgggccacgtcatacttccgccgcctcggctgtggtgccggtggctataataaacgggctacatgcgcgcgcacctacttctggtcgggaagggccgacacggctttaccaggtagttttttaccgaaagcgatggacctctctgBbsIBsmBIEsp3IMauBIBssHIIlacZ CDSsource6,9606,9807,0007,0207,0407,0607,0807,1007,120
    gcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggccgcgggcgactaggaaacgcttatgcgggtgcgctacccattgtcagaaccgccaaagcgatttatgaccgtccgcaaagcagtcataggggcaaatgtcccgccgaagcagaccctgacccacctagtcagcgactaatttatactacttttgccgttgggcaccagccgaatgccgPflMIlacZ CDSsource7,1407,1607,1807,2007,2207,2407,2607,280
    ggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataccactaaaaccgctatgcggcttgctagcggtcaagacatacttgccagaccagaaacggctggcgtgcggcgtaggtcgcgactgccttcgttttgtggtcgtcgtcaaaaaggtcaaggcaaataggcccgtttggtagcttcactggtcgcttatggacaaggcagtatcgctatlacZ CDSsource7,3007,3207,3407,3607,3807,4007,4207,4407,460
    acgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatgtgctcgaggacgtgacctaccaccgcgacctaccattcggcgaccgttcgccacttcacggagacctacagcgaggtgttccatttgtcaactaacttgacggacttgatggcgtcggcctctcgcggcccgttgagaccgagtgtcatgcgcatcacgttggcttgcgctggcgtacSacIEco53kIlacZ CDSsource7,4807,5007,5207,5407,5607,5807,6007,6207,640
    gtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagcagtcttcggcccgtgtagtcgcggaccgtcgtcaccgcagaccgccttttggagtcacactgcgaggggcggcgcagggtgcggtagggcgtagactggtggtcgctttacctaaaaacgtagctcgacccattattcgcaaccgttaaattggcggtcagtccgaaagaaagtgtclacZ CDSsource7,6607,6807,7007,7207,7407,7607,7807,8007,820
    atgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacggtacacctaaccgctattttttgttgacgactgcggcgacgcgctagtcaagtgggcacgtggcgacctattgctgtaaccgcattcacttcgctgggcgtaactgggattgcggacccagcttgcgaccttccgccgcccggtaatggtccggcttcgtcgcaacaacgtcacgtgcclacZ CDSsource7,8407,8607,8807,9007,9207,9407,9607,9808,000
    cagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactggtctatgtgaacgactacgccacgactaatgctggcgagtgcgcaccgtcgtagtccccttttggaataaatagtcggccttttggatggcctaactaccatcaccagtttaccgctaatggcaactacaacttcaccgctcgctatgtggcgtaggccgcgcctaaccggacttgaclacZ CDSsource8,0208,0408,0608,0808,1008,1208,1408,1608,180
    ccagctggcgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtataccccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaatggtcgaccgcgtccatcgtctcgcccatttgaccgagcctaatcccggcgttcttttgatagggctggcggaatgacggcggacaaaactggcgaccctagacggtaacagtctgtacatatggggcatgcagaagggctcgcttttgccagacgcgacgccctgcgcgcttaacttaBbsIPciIBsiWIBstZ17IlacZ CDSsource8,2008,2208,2408,2608,2808,3008,3208,3408,360
    tatggcccacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggataccgggtgtggtcaccgcgccgctgaaggtcaagttgtagtcggcgatgtcagttgtcgttgactacctttggtcggtagcggtagacgacgtgcgccttcttccgtgtaccgacttatagctgccaaaggtatacccctaaccaccgctgctgaggacctcgggcagtcatagccNdeIPfoIlacZ CDSsource8,3808,4008,4208,4408,4608,4808,5008,5208,540
    cggaattccagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataataataaccgggcaggccatgtctgcccgtatttcgcgtaaggaaatccattatgtactatttaaaaaacacaaacttttggatgttcggtttattctttttcttttacttttttatcatgccttaaggtcgactcgcggccagcgatggtaatggtcaaccagaccacagtttttattattattggcccgtccggtacagacgggcataaagcgcattcctttaggtaatacatgataaattttttgtgtttgaaaacctacaagccaaataagaaaaagaaaatgaaaaaatagtalacZ CDSsource8,5608,5808,6008,6208,6408,6608,6808,7008,720
    gggagcctacttcccgtttttcccgatttggctacatgacatcaaccatatcagcaaaagtgatacgggtattatttttgccgctatttctctgttctcgctattattccaaccgctgtttggtctgctttctgacaaactcggcaagctctaggcccactaagcgtcgcgccacttcccctcggatgaagggcaaaaagggctaaaccgatgtactgtagttggtatagtcgttttcactatgcccataataaaaacggcgataaagagacaagagcgataataaggttggcgacaaaccagacgaaagactgtttgagccgttcgagatccgggtgattcgcagcgcggtgaagsource8,7408,7608,7808,8008,8208,8408,8608,8808,900
    aacgctcgatgggagcgtcattggtgggcggggtaaccgtcgaaatcagtgtttacgcttccaatcgcaacaaaaaattcactgcaacactgaaaagcatacgaaaacgatgaagattgtacgagaaaccataaagtattttatccacaaagacacgtatagcagaaaagccaagttattgcgagctaccctcgcagtaaccacccgccccattggcagctttagtcacaaatgcgaaggttagcgttgttttttaagtgacgttgtgacttttcgtatgcttttgctacttctaacatgctctttggtatttcataaaataggtgtttctgtgcatatcgtcttttcggttcaatBsaAIBstEIIsource8,9208,9408,9608,9809,0009,0209,0409,060
    actcggcgataagttgtgtacacaagaataaaatcggccagattcagtgttgtcagaaataagaatgagccgctattcaacacatgtgttcttattttagccggtctaagtcacaacagtctttattcttBsrGIsource9,0809,1009,1209,140
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9143 bp65007000750080008500500100015002000sourcelacZ CDSbla CDS16.32-PP7TSeq4 (DNA)1.11-P2PSeq7 (DNA)2.13-MCPGFPSeq5 (DNA)2.36-Tub3-SeqUpstream (DNA)2.41 MCP-GFP Seq 4 (DNA),1.43-P2P38F1Down3 (DNA),3.32-TubUpSeq1 (DNA),+5promoter for bacteriophage T3 RNA polymerase,+142.2-MS2x24Seq1 (DNA)PciIhigh-copy-number ColE1/pMB1/pBR322/pUC origin of replication3.30-Carnegie4Seq5 (DNA)AhdIAclI1.101-P2PSeq14 (DNA)BclIBssHII,MauBIBbsIBsmBIEsp3I,PflMI1.102-P2PSeq15 (DNA)Eco53kISacIPciI,BstZ17I,BbsI,BsiWI1.6-P2PSeq3 (DNA),NdeI,PfoI1.10-P2PSeq6 (DNA),1.8-P2PSeq4 (DNA),1.41-P2P38F1Down1 (DNA),1.9-P2PSeq5 (DNA),+4

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    9143