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1898-8_FourGA20ox_guides_delcas9
  • Sequence Map
    Editing disabled on read-only sequence.
    ggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattcccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagpBR322_origin20406080100120140160
    tgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgcacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgSapIBspQI180200220240260280300320340
    tctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaBstZ17IpGEX_3_primer360380400420440460480500520
    ttcaccgtcatcaccgaaacgcgcgaggcagggtgccttgatgtgggcgccggcggtcgagtggcgacggcgcggcttgtccgcgccctggtagattgcctggccgtaggccagccatttttgagcggccagcggccgcgataggccgacgcgaagcggcggggcgtagggagcgaagtggcagtagtggctttgcgcgctccgtcccacggaactacacccgcggccgccagctcaccgctgccgcgccgaacaggcgcgggaccatctaacggaccggcatccggtcggtaaaaactcgccggtcgccggcgctatccggctgcgcttcgccgccccgcatccctcgcKasIMreINarISfoIPluTISgrAI540560580600620640660680700
    cagcgaccgaagggtaggcgctttttgcagctcttcggctgtgcgctggccagacagttatgcacaggccaggcgggttttaagagttttaataagttttaaagagttttaggcggaaaaatcgccttttttctcttttatatcagtcacttacatgtgtgaccggttcccaatggtcgctggcttcccatccgcgaaaaacgtcgagaagccgacacgcgaccggtctgtcaatacgtgtccggtccgcccaaaattctcaaaattattcaaaatttctcaaaatccgcctttttagcggaaaaaagagaaaatatagtcagtgaatgtacacactggccaagggttacAgeISapIBspQI720740760780800820840860
    tacggctttgggttcccaatgtacgggttccggttcccaatgtacggctttgggttcccaatgtacgtgctatccacaggaaacagaccttttcgacctttttcccctgctagggcaatttgccctagcatctgctccgtacattaggaaccggcggatgcttcgccctcgatcaatgccgaaacccaagggttacatgcccaaggccaagggttacatgccgaaacccaagggttacatgcacgataggtgtcctttgtctggaaaagctggaaaaaggggacgatcccgttaaacgggatcgtagacgaggcatgtaatccttggccgcctacgaagcgggagctagtEcoNI8809009209409609801,0001,0201,040
    ggttgcggtagcgcatgactaggatcgggccagcctgccccgcctcctccttcaaatcgtactccggcaggtcatttgacccgatcagcttgcgcacggtgaaacagaacttcttgaactctccggcgctgccactgcgttcgtagatcgtcttgaacaaccatctggcttctgcccaacgccatcgcgtactgatcctagcccggtcggacggggcggaggaggaagtttagcatgaggccgtccagtaaactgggctagtcgaacgcgtgccactttgtcttgaagaacttgagaggccgcgacggtgacgcaagcatctagcagaacttgttggtagaccgaagacgFspI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cttgcctgcggcgcggcgtgccaggcggtagagaaaacggccgatgccgggatcgatcaaaaagtaatcggggtgaaccgtcagcacgtccgggttcttgccttctgtgatctcgcggtacatccaatcagctagctcgatctcgatgtactccggccgcccggtttcgctctttgaacggacgccgcgccgcacggtccgccatctcttttgccggctacggccctagctagtttttcattagccccacttggcagtcgtgcaggcccaagaacggaagacactagagcgccatgtaggttagtcgatcgagctagagctacatgaggccggcgggccaaagcgagaaaBmtINheI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    acgatcttgtagcggctaatcaaggcttcaccctcggataccgtcaccaggcggccgttcttggccttcttcgtacgctgcatggcaacgtgcgtggtgtttaaccgaatgcaggtttctaccaggtcgtctttctgctttccgccatcggctcgccggcagaacttgagtacgttgctagaacatcgccgattagttccgaagtgggagcctatggcagtggtccgccggcaagaaccggaagaagcatgcgacgtaccgttgcacgcaccacaaattggcttacgtccaaagatggtccagcagaaagacgaaaggcggtagccgagcggccgtcttgaactcatgcaBciVIBsiWI1,4201,4401,4601,4801,5001,5201,5401,560
    ccgcaacgtgtggacggaacacgcggccgggcttgtctcccttcccttcccggtatcggttcatggattcggttagatgggaaaccgccatcagtaccaggtcgtaatcccacacactggccatgccggccggccctgcggaaacctctacgtgcccgtctggaagctcgtagcgggcgttgcacacctgccttgtgcgccggcccgaacagagggaagggaagggccatagccaagtacctaagccaatctaccctttggcggtagtcatggtccagcattagggtgtgtgaccggtacggccggccgggacgcctttggagatgcacgggcagaccttcgagcatcgcPflMI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gatcacctcgccagctcgtcggtcacgcttcgacagacggaaaacggccacgtccatgatgctgcgactatcgcgggtgcccacgtcatagagcatcggaacgaaaaaatctggttgctcgtcgcccttgggcggcttcctaatcgacggcgcaccggctgccggcggttgccggctagtggagcggtcgagcagccagtgcgaagctgtctgccttttgccggtgcaggtactacgacgctgatagcgcccacgggtgcagtatctcgtagccttgcttttttagaccaacgagcagcgggaacccgccgaaggattagctgccgcgtggccgacggccgccaacggcc1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gattctttgcggattcgatcagcggccgcttgccacgattcaccggggcgtgcttctgcctcgatgcgttgccgctgggcggcctgcgcggccttcaacttctccaccaggtcatcacccagcgccgcgccgatttgtaccgggccggatggtttgcgaccgctcacgccgattcctaagaaacgcctaagctagtcgccggcgaacggtgctaagtggccccgcacgaagacggagctacgcaacggcgacccgccggacgcgccggaagttgaagaggtggtccagtagtgggtcgcggcgcggctaaacatggcccggcctaccaaacgctggcgagtgcggctaag1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ctcgggcttgggggttccagtgccattgcagggccggcagacaacccagccgcttacgcctggccaaccgcccgttcctccacacatggggcattccacggcgtcggtgcctggttgttcttgattttccatgccgcctcctttagccgctaaaattcatctactcatttattcagagcccgaacccccaaggtcacggtaacgtcccggccgtctgttgggtcggcgaatgcggaccggttggcgggcaaggaggtgtgtaccccgtaaggtgccgcagccacggaccaacaagaactaaaaggtacggcggaggaaatcggcgattttaagtagatgagtaaataagt2,1202,1402,1602,1802,2002,2202,2402,260
    tttgctcatttactctggtagctgcgcgatgtattcagatagcagctcggtaatggtcttgccttggcgtaccgcgtacatcttcagcttggtgtgatcctccgccggcaactgaaagttgacccgcttcatggctggcgtgtctgccaggctggccaacgttgcagccttgctgaaacgagtaaatgagaccatcgacgcgctacataagtctatcgtcgagccattaccagaacggaaccgcatggcgcatgtagaagtcgaaccacactaggaggcggccgttgactttcaactgggcgaagtaccgaccgcacagacggtccgaccggttgcaacgtcggaacgacAclI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctgcgtgcgctcggacggccggcacttagcgtgtttgtgcttttgctcattttctctttacctcattaactcaaatgagttttgatttaatttcagcggccagcgcctggacctcgcgggcagcgtcgccctcgggttctgattcaagaacggttgtgccggcggcggcagtgccgacgcacgcgagcctgccggccgtgaatcgcacaaacacgaaaacgagtaaaagagaaatggagtaattgagtttactcaaaactaaattaaagtcgccggtcgcggacctggagcgcccgtcgcagcgggagcccaagactaagttcttgccaacacggccgccgccgtcacggPflMI2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgggtagctcacgcgctgcgtgatacgggactcaagaatgggcagctcgtacccggccagcgcctcggcaacctcaccgccgatgcgcgtgcctttgatcgcccgcgacacgacaaaggccgcttgtagccttccatccgtgacctcaatgcgctgcttaaccagctccaccaggacccatcgagtgcgcgacgcactatgccctgagttcttacccgtcgagcatgggccggtcgcggagccgttggagtggcggctacgcgcacggaaactagcgggcgctgtgctgtttccggcgaacatcggaaggtaggcactggagttacgcgacgaattggtcgaggtggtcc2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcggcggtggcccatatgtcgtaagggcttggctgcaccggaatcagcacgaagtcggctgccttgatcgcggacacagccaagtccgccgcctggggcgctccgtcgatcactacgaagtcgcgccggccgatggccttcacgtcgcggtcaatcgtcgggcggtcgatgccgaagccgccaccgggtatacagcattcccgaaccgacgtggccttagtcgtgcttcagccgacggaactagcgcctgtgtcggttcaggcggcggaccccgcgaggcagctagtgatgcttcagcgcggccggctaccggaagtgcagcgccagttagcagcccgccagctacggct2,8202,8402,8602,8802,9002,9202,9402,960
    caacggttagcggttgatcttcccgcacggccgcccaatcgcgggcactgccctggggatcggaatcgactaacagaacatcggccccggcgagttgcagggcgcgggctagatgggttgcgatggtcgtcttgcctgacccgcctttctggttaagtacagcgataaccttcatgttgccaatcgccaactagaagggcgtgccggcgggttagcgcccgtgacgggacccctagccttagctgattgtcttgtagccggggccgctcaacgtcccgcgcccgatctacccaacgctaccagcagaacggactgggcggaaagaccaattcatgtcgctattggaagtaBsaBI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gcgttccccttgcgtatttgtttatttactcatcgcatcatatacgcagcgaccgcatgacgcaagctgttttactcaaatacacatcacctttttagacggcggcgctcggtttcttcagcggccaagctggccggccaggccgccagcttggcatcagacaaaccggccaggacgcaaggggaacgcataaacaaataaatgagtagcgtagtatatgcgtcgctggcgtactgcgttcgacaaaatgagtttatgtgtagtggaaaaatctgccgccgcgagccaaagaagtcgccggttcgaccggccggtccggcggtcgaaccgtagtctgtttggccggtcct3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tttcatgcagccgcacggttgagacgtgcgcgggcggctcgaacacgtacccggccgcgatcatctccgcctcgatctcttcggtaatgaaaaacggttcgtcctggccgtcctggtgcggtttcatgcttgttcctcttggcgttcattctcggcggccgccagggcgtcggccaaagtacgtcggcgtgccaactctgcacgcgcccgccgagcttgtgcatgggccggcgctagtagaggcggagctagagaagccattactttttgccaagcaggaccggcaggaccacgccaaagtacgaacaaggagaaccgcaagtaagagccgccggcggtcccgcagccgg3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tcggtcaatgcgtcctcacggaaggcaccgcgccgcctggcctcggtgggcgtcacttcctcgctgcgctcaagtgcgcggtacagggtcgagcgatgcacgccaagcagtgcagccgcctctttcacggtgcggccttcctggtcgatcagctcgcgggcgtgcgcgatctgtgagccagttacgcaggagtgccttccgtggcgcggcggaccggagccacccgcagtgaaggagcgacgcgagttcacgcgccatgtcccagctcgctacgtgcggttcgtcacgtcggcggagaaagtgccacgccggaaggaccagctagtcgagcgcccgcacgcgctagacac3,5203,5403,5603,5803,6003,6203,6403,660
    ccggggtgagggtagggcgggggccaaacttcacgcctcgggccttggcggcctcgcgcccgctccgggtgcggtcgatgattagggaacgctcgaactcggcaatgccggcgaacacggtcaacaccatgcggccggccggcgtggtggtgtcggcccacggctctgccaggctggccccactcccatcccgcccccggtttgaagtgcggagcccggaaccgccggagcgcgggcgaggcccacgccagctactaatcccttgcgagcttgagccgttacggccgcttgtgccagttgtggtacgccggccggccgcaccaccacagccgggtgccgagacggtccga3,6803,7003,7203,7403,7603,7803,8003,8203,840
    acgcaggcccgcgccggcctcctggatgcgctcggcaatgtccagtaggtcgcgggtgctgcgggccaggcggtctagcctggtcactgtcacaacgtcgccagggcgtaggtggtcaagcatcctggccagctccgggcggtcgcgcctggtgccggtgatcttctcggaaaactgcgtccgggcgcggccggaggacctacgcgagccgttacaggtcatccagcgcccacgacgcccggtccgccagatcggaccagtgacagtgttgcagcggtcccgcatccaccagttcgtaggaccggtcgaggcccgccagcgcggaccacggccactagaagagccttttg3,8603,8803,9003,9203,9403,9603,9804,0004,020
    agcttggtgcagccggccgcgtgcagttcggcccgttggttggtcaagtcctggtcgtcggtgctgacgcgggcatagcccagcaggccagcggcggcgctcttgttcatggcgtaatgtctccggttctagtcgcaagtattctactttatgcgactaaaacacgcgacaagaatcgaaccacgtcggccggcgcacgtcaagccgggcaaccaaccagttcaggaccagcagccacgactgcgcccgtatcgggtcgtccggtcgccgccgcgagaacaagtaccgcattacagaggccaagatcagcgttcataagatgaaatacgctgattttgtgcgctgttctt4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aacgccaggaaaagggcagggcggcagcctgtcgcgtaacttaggacttgtgcgacatgtcgttttcagaagacggctgcactgaacgtcagaagccgactgcactatagcagcggaggggttggatcaaagtactttgatcccgaggggaaccctgtggttggcatgcacatacttgcggtccttttcccgtcccgccgtcggacagcgcattgaatcctgaacacgctgtacagcaaaagtcttctgccgacgtgacttgcagtcttcggctgacgtgatatcgtcgcctccccaacctagtttcatgaaactagggctccccttgggacaccaaccgtacgtgtatgSphI4,2204,2404,2604,2804,3004,3204,3404,360
    aaatggacgaacggataaaccttttcacgcccttttaaatatccgttattctaataaacgctcttttctcttaggtttacccgccaatatatcctgtcaaacactgatagtttaaactgaaggcgggaaacgacaatctgatccaagctcaagctgctctagcattcgccattcatttacctgcttgcctatttggaaaagtgcgggaaaatttataggcaataagattatttgcgagaaaagagaatccaaatgggcggttatataggacagtttgtgactatcaaatttgacttccgccctttgctgttagactaggttcgagttcgacgagatcgtaagcggtaagtPmeI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttcgacttgccttccgcacaatacaccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacggttcgaagctgaacggaaggcgtgttatgtFspIHindIIIlacZ_aU6 promoterM13_pUC_fwd_primerM13_f...imer4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tcatttcttcttagctttttttcttcttcttcgttcatacagtttttttttgtttatcagcttacattttcttgaaccgtagctttcgttttcttctttttaactttccattcggagtttttgtatcttgtttcatagtttgtcccaggattagaatgattaggcatcgaaccttagtaaagaagaatcgaaaaaaagaagaagaagcaagtatgtcaaaaaaaaacaaatagtcgaatgtaaaagaacttggcatcgaaagcaaaagaagaaaaattgaaaggtaagcctcaaaaacatagaacaaagtatcaaacagggtcctaatcttactaatccgtagcttggaaU6 promoter4,7404,7604,7804,8004,8204,8404,8604,8804,900
    caagaatttgattgaataaaacatcttcattcttaagatatgaagataatcttcaaaaggcccctgggaatctgaaagaagagaagcaggcccatttatatgggaaagaacaatagtatttcttatataggcccatttaagttgaaaacaatcttcaaaagtcccacatcgcttagttcttaaactaacttattttgtagaagtaagaattctatacttctattagaagttttccggggacccttagactttcttctcttcgtccgggtaaatataccctttcttgttatcataaagaatatatccgggtaaattcaacttttgttagaagttttcagggtgtagcgaatU6 promoter4,9204,9404,9604,9805,0005,0205,0405,060
    gataagaaaacgaagctgagtttatatacagctagagtcgaagtagtgattgTATGATTTTGAAAACAAACGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttttgcaaaattttccagatcgactattcttttgcttcgactcaaatatatgtcgatctcagcttcatcactaacATACTAAAACTTTTGTTTGCcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaaacgttttaaaaggtctagctU6 promoterguide1_20ox5F_prime...mology5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tttcttcttcctctgttcttcggcgttcaatttctggggttttctcttcgttttctgtaactgaaacctaaaatttgacctaaaaaaaatctcaaataatatgattcagtggttttgtacttttcagttagttgagttttgcagttccgatgagataaaccaatattaatccaaaaaagaagaaggagacaagaagccgcaagttaaagaccccaaaagagaagcaaaagacattgactttggattttaaactggattttttttagagtttattatactaagtcaccaaaacatgaaaagtcaatcaactcaaaacgtcaaggctactctatttggttataattaggtttSspISe...F5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctactgcagcctgacagacaaatgaggatgcaaacaattttaaagtttatctaacgctagctgttttgtttcttctctctggtgcaccaacgacggcgttttctcaatcataaagaggcttgttttacttaaggccaataatgttgatggatcgaaagaagagggcttttaataagatgacgtcggactgtctgtttactcctacgtttgttaaaatttcaaatagattgcgatcgacaaaacaaagaagagagaccacgtggttgctgccgcaaaagagttagtatttctccgaacaaaatgaattccggttattacaactacctagctttcttctcccgaaaattattBmtINheIPstIU6-29p-RSeco...q F5,4405,4605,4805,5005,5205,5405,5605,5805,600
    acgagcccgtttaagctgtaaacgatgtcaaaaacatcccacatcgttcagttgaaaatagaagctctgtttatatATTGGTAGAGTCGACTAAGAGATgAATTTATTTAGTGGCTGAACgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaatgctcgggcaaattcgacatttgctacagtttttgtagggtgtagcaagtcaacttttatcttcgagacaaatataTAACCATCTCAGCTGATTCTCTAcTTAAATAAATCACCGACTTGcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaacttAclISalIBanIIguide2_20ox3Forward...mology5,6205,6405,6605,6805,7005,7205,7405,760
    aaagtggcaccgagtcggtgcttttttttggatagaatttcccagcttttttgcgtgtttcagctctcatgatccttggccaatgggtgtagtaaattttctgcacattcattggatggaaaataatggttttagctttagggaataagaaaagtgtataggaaggggatttttgtttcaccgtggctcagccacgaaaaaaaacctatcttaaagggtcgaaaaaacgcacaaagtcgagagtactaggaaccggttacccacatcatttaaaagacgtgtaagtaacctaccttttattaccaaaatcgaaatcccttattcttttcacatatccttcccctaaaaacBspHIBsrGI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tacaatcacatttgaattaggtctttgaaatgacagggaatgaggacatatgatgagacggtcattgttttagttccaccacgattatatttgaaatttacgtgagtgtgagtgagacttgcataagaaaataaaatctttagttgggaaaaaattcaataatataaatgggcttatgttagtgtaaacttaatccagaaactttactgtcccttactcctgtatactactctgccagtaacaaaatcaaggtggtgctaatataaactttaaatgcactcacactcactctgaacgtattcttttattttagaaatcaacccttttttaagttattatatttacccgaaThird Guide Seq F5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gagaaggaagcgagggataggcctttttctaaaataggcccatttaagctattaacaatcttcaaaagtaccacagcgcttaggtaaagaaagcagctgagtttatatatggttagagacgaagtagtgatgTTGAGGAGATGGGGGAACAAgttttagagctagaaatagcaagctcttccttcgctccctatccggaaaaagattttatccgggtaaattcgataattgttagaagttttcatggtgtcgcgaatccatttctttcgtcgactcaaatatataccaatctctgcttcatcactacAACTCCTCTACCCCCTTGTTcaaaatctcgatctttatcgttcAfeIStuIBpu10Iguide3_20ox2F Prime...mology6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttttggcaaaaattttcagattttttcttcatctgtagatttctgggtttttttttccgtttcgtgaatcataagtgaagttttggatgcaaatctgcgcgaaaaaagttggacctgcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaaaccgtttttaaaagtctaaaaaagaagtagacatctaaagacccaaaaaaaaaggcaaagcacttagtattcacttcaaaacctacgtttagacgcgcttttttcaacctggacgBsaBI6,3206,3406,3606,3806,4006,4206,4406,460
    aatgagcttatttagatagctaagacaaagtgattggtccgttcgacttgccttccgcacaatacatcatttcttcttagctttttttcttcttcttcgttcatacagtttttttttgtttatcagcttacattttcttgaaccgtagctttcgttttcttctttttaactttccttactcgaataaatctatcgattctgtttcactaaccaggcaagctgaacggaaggcgtgttatgtagtaaagaagaatcgaaaaaaagaagaagaagcaagtatgtcaaaaaaaaacaaatagtcgaatgtaaaagaacttggcatcgaaagcaaaagaagaaaaattgaaagg6,4806,5006,5206,5406,5606,5806,6006,6206,640
    attcggagtttttgtatcttgtttcatagtttgtcccaggattagaatgattaggcatcgaaccttcaagaatttgattgaataaaacatcttcattcttaagatatgaagataatcttcaaaaggcccctgggaatctgaaagaagagaagcaggcccatttatatgggaaagataagcctcaaaaacatagaacaaagtatcaaacagggtcctaatcttactaatccgtagcttggaagttcttaaactaacttattttgtagaagtaagaattctatacttctattagaagttttccggggacccttagactttcttctcttcgtccgggtaaatataccctttct6,6606,6806,7006,7206,7406,7606,7806,8006,820
    acaatagtatttcttatataggcccatttaagttgaaaacaatcttcaaaagtcccacatcgcttagataagaaaacgaagctgagtttatatacagctagagtcgaagtagtgattgATGGTCCTTTTAGTCTTTATgttttagagctagaaatagcaagttaaaataaggctatgttatcataaagaatatatccgggtaaattcaacttttgttagaagttttcagggtgtagcgaatctattcttttgcttcgactcaaatatatgtcgatctcagcttcatcactaacTACCAGGAAAATCAGAAATAcaaaatctcgatctttatcgttcaattttattccgattracRNAReverse Pr... Homologyguide4_20ox16,8406,8606,8806,9006,9206,9406,9606,9807,000
    gtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttttgcaaaattttccagatcgatttcttcttcctctgttcttcggcgttcaatttctggggttttctcttcgttttctgtaactgaaacctaaaatttgacctaaaaaaaatctcaaataatatgattcagcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaaacgttttaaaaggtctagctaaagaagaaggagacaagaagccgcaagttaaagaccccaaaagagaagcaaaagacattgactttggattttaaactggattttttttagagtttattatactaagtcU6 TermtracRNA7,0207,0407,0607,0807,1007,1207,1407,160
    tggttttgtacttttcagttagttgagttttgcagttccgatgagataaaccaataccatggttatagaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaaaccaaaacatgaaaagtcaatcaactcaaaacgtcaaggctactctatttggttatggtaccaatatcttaagcattagtacagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattNcoIEcoRIU6 Termlac_promotercas9_delM13_pUC_rev_primerremove_cas97,1807,2007,2207,2407,2607,2807,3007,3207,340
    tgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattggctagagcagcttgccaacatggtggagcacgacactctcgtctactccaagaaactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataaccgatctcgtcgaacggttgtaccacctcgtgctgtgagagcagatgaggttcttCaMVd35S_promoter7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tatcaaagatacagtctcagaagaccaaagggctattgagacttttcaacaaagggtaatatcgggaaacctcctcggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatcatagtttctatgtcagagtcttctggtttcccgataactctgaaaagttgtttcccattatagccctttggaggagcctaaggtaacgggtcgatagacagtgaagtagttttcctgtcatcttttccttccaccgtggatgtttacggtagtaacgctatttcctttccgatagCaMVd35S_promoter7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gttcaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgataacatggtggagcacgacactctcgtctactccaagaatatcaaagatacagtctcagaagcaagttctacggagacggctgtcaccagggtttctacctgggggtgggtgctcctcgtagcacctttttcttctgcaaggttggtgcagaagtttcgttcacctaactacactattgtaccacctcgtgctgtgagagcagatgaggttcttatagtttctatgtcagagtcttcCaMVd35S_promoter7,7207,7407,7607,7807,8007,8207,8407,860
    accaaagggctattgagacttttcaacaaagggtaatatcgggaaacctcctcggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatcgttcaagatgcctctgccgacagtggtttcccgataactctgaaaagttgtttcccattatagccctttggaggagcctaaggtaacgggtcgatagacagtgaagtagttttcctgtcatcttttccttccaccgtggatgtttacggtagtaacgctatttcctttccgatagcaagttctacggagacggctgtcCaMVd35S_promoter7,8807,9007,9207,9407,9607,9808,0008,0208,040
    tggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagaccttcctctatataaggaagttcatttcatttggaccagggtttctacctgggggtgggtgctcctcgtagcacctttttcttctgcaaggttggtgcagaagtttcgttcacctaactacactatagaggtgactgcattccctactgcgtgttagggtgataggaagcgttctggaaggagatatattccttcaagtaaagtaaaccEcoRVCaMVd35S_promoter8,0608,0808,1008,1208,1408,1608,1808,2008,220
    agaggacacgctgaaatcaccagtctctctctacaaatctatctctctcgagctttcgcagatcccggggggcaatgagatatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgtctcctgtgcgactttagtggtcagagagagatgtttagatagagagagctcgaaagcgtctagggccccccgttactctatactttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcSmaITliIXhoIXmaIZraIAatIITspMIPaeR7IhygroB (variant)8,2408,2608,2808,3008,3208,3408,3608,3808,400
    aagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggagtttagcgagagcctgacctattgttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaacccctcaaatcgctctcggactggataachygroB (variant)8,4208,4408,4608,4808,5008,5208,5408,560
    catctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctacaaccggtcgcggaggctatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagatgttggccagcgcctccgatacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcaAgeIAsiSIRsrIIDraIIIhygroB (variant)8,5808,6008,6208,6408,6608,6808,7008,7208,740
    gatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggctaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgccDraIIIhygroB (variant)8,7608,7808,8008,8208,8408,8608,8808,9008,920
    acaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccacgactccgggcgtatgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggtgctgaggcccgcathygroB (variant)8,9408,9608,9809,0009,0209,0409,0609,0809,100
    tatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaaatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatctthygroB (variant)9,1209,1409,1609,1809,2009,2209,2409,260
    gtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaagaaatagagtagatgccgaccggatctgtcgatcgacaagctcgagtttctccataataatgtgtgagtagttcccagataagggaattagggttcctatagggtttcgctcatgtgttgagcatcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttctttatctcatctacggctggcctagacagctagctgttcgagctcaaagaggtattattacacactcatcaagggtctattcccttaatcccaaggatatcccaaagcgagtacacaactcgtaAloITliIXhoIPspXIPaeR7IhygroB (variant)9,2809,3009,3209,3409,3609,3809,4009,4209,440
    ataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtactaaaatccagatcccccgaattaattcggcgttaattcagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaatttgttattctttgggaatcatacataaacataaacattttatgaagatagttattttaaagattaaggattttggttttaggtcatgattttaggtctagggggcttaattaagccgcaattaagtcatgtaatttttgcaggcgttacacaataattcaacagattcgcagttaaaca9,4609,4809,5009,5209,5409,5609,5809,6009,620
    ttacaccacaatatatcctgccaccagccagccaacagctccccgaccggcagctcggcacaaaatcaccactcgatacaggcagcccatcagtccgggacggcgtcagcgggagagccgttgtaaggcggcagactttgctcatgttaccgatgctattcggaagaacggcaacaatgtggtgttatataggacggtggtcggtcggttgtcgaggggctggccgtcgagccgtgttttagtggtgagctatgtccgtcgggtagtcaggccctgccgcagtcgccctctcggcaacattccgccgtctgaaacgagtacaatggctacgataagccttcttgccgttg9,6409,6609,6809,7009,7209,7409,7609,7809,800
    taagctgccgggtttgaaacacggatgatctcgcggagggtagcatgttgattgtaacgatgacagagcgttgctgcctgtgatcaccgcggtttcaaaatcggctccgtcgatactatgttatacgccaactttgaaaacaactttgaaaaagctgttttctggtatttaaggtattcgacggcccaaactttgtgcctactagagcgcctcccatcgtacaactaacattgctactgtctcgcaacgacggacactagtggcgccaaagttttagccgaggcagctatgatacaatatgcggttgaaacttttgttgaaactttttcgacaaaagaccataaattccaBclISacII9,8209,8409,8609,8809,9009,9209,9409,960
    tttagaatgcaaggaacagtgaattggagttcgtcttgttataattagcttcttggggtatctttaaatactgtagaaaagaggaaggaaataataaatggctaaaatgagaatatcaccggaattgaaaaaactgatcgaaaaataccgctgcgtaaaagatacggaaggaatgaaatcttacgttccttgtcacttaacctcaagcagaacaatattaatcgaagaaccccatagaaatttatgacatcttttctccttcctttattatttaccgattttactcttatagtggccttaacttttttgactagctttttatggcgacgcattttctatgccttccttacPsiIKanR19,98010,00010,02010,04010,06010,08010,10010,12010,140
    tctcctgctaaggtatataagctggtgggagaaaatgaaaacctatatttaaaaatgacggacagccggtataaagggaccacctatgatgtggaacgggaaaaggacatgatgctatggctggaaggaaagctgcctgttccaaaggtcctgcactttgaacggcatgatggctagaggacgattccatatattcgaccaccctcttttacttttggatataaatttttactgcctgtcggccatatttccctggtggatactacaccttgcccttttcctgtactacgataccgaccttcctttcgacggacaaggtttccaggacgtgaaacttgccgtactaccgaPpuMIBpu10IKanR110,16010,18010,20010,22010,24010,26010,28010,30010,320
    ggagcaatctgctcatgagtgaggccgatggcgtcctttgctcggaagagtatgaagatgaacaaagccctgaaaagattatcgagctgtatgcggagtgcatcaggctctttcactccatcgacatatcggattgtccctatacgaatagcttagacagccgcttagccgaattcctcgttagacgagtactcactccggctaccgcaggaaacgagccttctcatacttctacttgtttcgggacttttctaatagctcgacatacgcctcacgtagtccgagaaagtgaggtagctgtatagcctaacagggatatgcttatcgaatctgtcggcgaatcggcttaaBlpIBspHIKanR110,34010,36010,38010,40010,42010,44010,46010,48010,500
    ggattacttactgaataacgatctggccgatgtggattgcgaaaactgggaagaagacactccatttaaagatccgcgcgagctgtatgattttttaaagacggaaaagcccgaagaggaacttgtcttttcccacggcgacctgggagacagcaacatctttgtgaaagatggccctaatgaatgacttattgctagaccggctacacctaacgcttttgacccttcttctgtgaggtaaatttctaggcgcgctcgacatactaaaaaatttctgccttttcgggcttctccttgaacagaaaagggtgccgctggaccctctgtcgttgtagaaacactttctaccgKanR110,52010,54010,56010,58010,60010,62010,64010,660
    aaagtaagtggctttattgatcttgggagaagcggcagggcggacaagtggtatgacattgccttctgcgtccggtcgatcagggaggatatcggggaagaacagtatgtcgagctattttttgacttactggggatcaagcctgattgggagaaaataaaatattatattttactttcattcaccgaaataactagaaccctcttcgccgtcccgcctgttcaccatactgtaacggaagacgcaggccagctagtccctcctatagccccttcttgtcatacagctcgataaaaaactgaatgacccctagttcggactaaccctcttttattttataatataaaatgSspIEcoRVKanR110,68010,70010,72010,74010,76010,78010,80010,82010,840
    tggatgaattgttttagtacctagaatgcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgacctacttaacaaaatcatggatcttacgtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacNsiIKanR1pBR322_origin10,86010,88010,90010,92010,94010,96010,98011,00011,020
    tttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcapBR322_origin11,04011,06011,08011,10011,12011,14011,16011,18011,200
    ggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctpBR322_origin11,22011,24011,26011,28011,30011,32011,34011,360
    gaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaBciVIpBR322_origin11,38011,40011,42011,440
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    11453 bp8500900095001000010500110005001000150020002500pGEX_3_primerpMOD_t1suf_F (DNA)GWI_141_F (DNA)GWI_160_R (DNA)Gibson_piece_1_F2 (DNA)Gibson_piece_2_R2 (DNA)M13(-47)_R (DNA)M13F_Long (DNA)pGREEN_Fw (DNA)936-p745 (DNA)pDONR-IAA28.t10-stop_F (DNA),pMOD_t1suf_R (DNA),Gibson_piece_2_R1 (DNA),+4KasI,NarI,SfoI,MreI,SgrAI,PluTI,BspQI,SapIAgeI,EcoNIFspINheIBmtI,BciVI,BsiWIPflMIAclIPflMIDraIII,AgeI,AsiSIhygroB (variant),RsrIIDraIIIHyg_F (DNA)PaeR7IPspXITliI,XhoI,AloIBclI,SacIIPsiIBpu10I,PpuMI,BspHIKanR1,BlpIEcoRV,SspI,NsiIpBR322_origin,BciVI

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    11453