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XZ031 CMV-TO-CCR6-ADAR2dd(E488Q, T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatATGAGCGGAGAGAGCATGAACTTCAGTGATGTGTTttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctaTACTCGCCTCTCTCGTACTTGAAGTCACTACACAAClaIPmeIAflIIBspDIHindIIIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28SG30ES32MN34FS36DV38F760-3186Signal/FlagCCR6CMV promot...th TetOs)homologhomolog720740760780800820840860
    CGATTCCAGTGAGGACTACTTCGTGTCCGTCAATACATCCTACTACAGCGTAGATTCTGAGATGCTGCTTTGCTCCCTGCAGGAGGTGCGCCAGTTCTCCCGACTCTTCGTTCCCATCGCGTATTCACTCATCTGCGTCTTTGGCCTCCTGGGGAATATCTTGGTAGTCATCACAGCTAAGGTCACTCCTGATGAAGCACAGGCAGTTATGTAGGATGATGTCGCATCTAAGACTCTACGACGAAACGAGGGACGTCCTCCACGCGGTCAAGAGGGCTGAGAAGCAAGGGTAGCGCATAAGTGAGTAGACGCAGAAACCGGAGGACCCCTTATAGAACCATCAGTAGTGTSbfIEcoNID40SS42ED44YF46VS48VN50TS52YY54SV56DS58EM60LL62CS64LQ66EV68RQ70FS72RL74FV76PI78AY80SL82IC84VF86GL88LG90NI92LV94VI96T760-3186CCR68809009209409609801,0001,0201,040
    TTTGCTTTTTATAAAAAGGCCAGATCCATGACAGACGTCTACCTGCTTAATATGGCCATTGCAGACATCCTGTTCGTCCTGACGCTCCCATTCTGGGCTGTGAGTCACGCTACCGGGGCCTGGGTGTTCAGTAATGCAACCTGCAAGCTGTTGAAGGGAATTTATGCCATCAACTAAACGAAAAATATTTTTCCGGTCTAGGTACTGTCTGCAGATGGACGAATTATACCGGTAACGTCTGTAGGACAAGCAGGACTGCGAGGGTAAGACCCGACACTCAGTGCGATGGCCCCGGACCCACAAGTCATTACGTTGGACGTTCGACAACTTCCCTTAAATACGGTAGTTGAMscIPsiIF98AF100YK102KA104RS106MT108DV110YL112LN114MA116IA118DI120LF122VL124TL126PF128WA130VS132HA134TG136AW138VF140SN142AT144CK146LL148KG150IY152AI154N760-3186CCR61,0601,0801,1001,1201,1401,1601,1801,2001,220
    TTAATTGCGGTATGCTGCTGCTGACTTGCATAAGTATGGACAGATACATCGCCATAGTACAGGCAACAAAATCCTTCAGACTGAGGTCTCGAACGCTCCCTAGGTCTAAAATCATTTGTCTTGTTGTATGGGGCTTGAGCGTGATTATTTCCAGCAGTACTTTCGTGTTTAACCAAATTAACGCCATACGACGACGACTGAACGTATTCATACCTGTCTATGTAGCGGTATCATGTCCGTTGTTTTAGGAAGTCTGACTCCAGAGCTTGCGAGGGATCCAGATTTTAGTAAACAGAACAACATACCCCGAACTCGCACTAATAAAGGTCGTCATGAAAGCACAAATTGGTBsaIAvrIIF156NC158GM160LL162LT164CI166SM168DR170YI172AI174VQ176AT178KS180FR182LR184SR186TL188PR190SK192II194CL196VV198WG200LS202VI204IS206SS208TF210VF212NQ214760-3186CCR61,2401,2601,2801,3001,3201,3401,3601,3801,400
    GAAGTACAACACGCAGGGCAGCGATGTGTGTGAGCCCAAGTACCAGACGGTATCTGAGCCTATCAGGTGGAAGCTGTTGATGCTGGGTTTGGAATTGCTGTTCGGCTTTTTTATCCCACTCATGTTTATGATTTTTTGTTATACCTTCATCGTGAAGACCCTGGTCCAGGCACAACTTCATGTTGTGCGTCCCGTCGCTACACACACTCGGGTTCATGGTCTGCCATAGACTCGGATAGTCCACCTTCGACAACTACGACCCAAACCTTAACGACAAGCCGAAAAAATAGGGTGAGTACAAATACTAAAAAACAATATGGAAGTAGCACTTCTGGGACCAGGTCCGTGTTAloIEcoNIKY216NT218QG220SD222VC224EP226KY228QT230VS232EP234IR236WK238LL240ML242GL244EL246LF248GF250FI252PL254MF256MI258FC260YT262FI264VK266TL268VQ270AQ272760-3186CCR61,4201,4401,4601,4801,5001,5201,5401,560
    AACTCTAAGCGACACAAGGCCATCCGGGTAATCATCGCTGTTGTGCTGGTATTTCTGGCTTGCCAGATCCCTCATAATATGGTCCTTCTGGTAACGGCAGCCAACCTGGGCAAAATGAATCGCTCATGTCAGTCAGAAAAGCTTATCGGGTACACCAAAACGGTTACGGAGGTTCTTGAGATTCGCTGTGTTCCGGTAGGCCCATTAGTAGCGACAACACGACCATAAAGACCGAACGGTCTAGGGAGTATTATACCAGGAAGACCATTGCCGTCGGTTGGACCCGTTTTACTTAGCGAGTACAGTCAGTCTTTTCGAATAGCCCATGTGGTTTTGCCAATGCCTCCAAGHindIIINS274KR276HK278AI280RV282II284AV286VL288VF290LA292CQ294IP296HN298MV300LL302VT304AA306NL308GK310MN312RS314CQ316SE318KL320IG322YT324KT326VT328EV330760-3186CCR61,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGGCGTTCCTGCATTGTTGCCTTAACCCTGTGCTGTACGCATTTATAGGTCAGAAGTTTCGAAACTACTTCCTGAAGATTCTCAAAGACCTCTGGTGTGTAAGAAGAAAGTATAAAAGTAGTGGATTTTCATGCGCAGGACGCTACTCCGAAAACATTTCCAGACAGACCAGTGAACCGCAAGGACGTAACAACGGAATTGGGACACGACATGCGTAAATATCCAGTCTTCAAAGCTTTGATGAAGGACTTCTAAGAGTTTCTGGAGACCACACATTCTTCTTTCATATTTTCATCACCTAAAAGTACGCGTCCTGCGATGAGGCTTTTGTAAAGGTCTGTCTGGTCACTFspIBstBILA332FL334HC336CL338NP340VL342YA344FI346GQ348KF350RN352YF354LK356IL358KD360LW362CV364RR366KY368KS370SG372FS374CA376GR378YS380EN382IS384RQ386TS388E760-3186CCR61,7601,7801,8001,8201,8401,8601,8801,9001,920
    GACGGCCGATAACGATAACGCCTCCTCCTTTACCATGGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACCTGCCGGCTATTGCTATTGCGGAGGAGGAAATGGTACCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGAgeIKpnIAcc65IBstEIIT390AD392ND394NA396SS398FT400MG402TG404AL406HL408DQ410TP412SR414QP416IP418SE420GL422QL424HL426PQ428VL430AD432AV434SR436LV438LG440KF442GD444LT446D760-3186hADAR2 cat domainCCR6homolog1,9401,9601,9802,0002,0202,0402,0602,0802,100
    AACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAN448FS450SP452HA454RR456KV458LA460GV462VM464TT466GT468DV470KD472AK474VI476SV478ST480GT482KC484IN486GE488YM490SD492RG494LA496LN498DC500HA502EI504I760-3186hADAR2 cat domain2,1202,1402,1602,1802,2002,2202,2402,260
    CTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAABclIBsrGIS506RR508SL510LR512FL514YT516QL518EL520YL522NN524KD526DQ528KR530SI532FQ534KS536ER538GG540FR542LK544EN546VQ548FH550LY552IS554TS556PC558GD560AR562IF564760-3186hADAR2 cat domain2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGPfoISP566HE568PI570LE572EP574AD576RH578PN580RK582AR584GQ586LR588TK590IE592SG594QG596TI598PV600RS602NA604SI606QA608WD610GV612LQ614GE616RL618LT620MS622760-3186hADAR2 cat domain2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGAAvaIBlpITliIXhoIBamHIBsoBIPpuMIPaeR7ICS624DK626IA628RW630NV632VG634IQ636GS638LL640SI642FV644EP646IY648FS650SI652IL654GS656LY658HG660DH662LS664RA666MY668QR670IS672NI674ED676LP678PL680760-3186hADAR2 cat domain2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTTGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAAMluIYT682LN684KP686LL688SG690IS692NA694EA696RQ698PG700KA702PN704FS706VN708WT710VG712DS714AI716EV718IN720AT722TG724KD726EL728GR730AS732RL734CK736HA738L760-3186hADAR2 cat domain2,8202,8402,8602,8802,9002,9202,9402,960
    GTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCY740CR742WM744RV746HG748KV750PS752HL754LR756SK758IT760KP762NV764YH766ES768KL770AA772KE774YQ776AA778KA780RL782FT784AF786IK788AG790LG792AW794VE796K760-3186hADAR2 cat domain2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacApaIAvaIBclIPmeITliIXhoIBsoBIPspXIPaeR7IPspOMI*1P798TE800QD802QF804SL806TP808*760-3186BGH polyadenylation sequencehADAR2 cat domainc-myc epitopePolyhi...e tag3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgaccNspISphIBGH polyadenylation sequence3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgcccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggClaIBspDIBGH pA3,5203,5403,5603,5803,6003,6203,6403,660
    agcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttccttcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggaKflINaeIPpuMINgoMIVFRT3,6803,7003,7203,7403,7603,7803,8003,8203,840
    attccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgttaaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcaBarIMscIXbaIHygromycinFRT3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacccctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggEcoRIDraIIIHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcaacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtNdeIPvuIAsiSIRsrIIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    ctggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctcDraIIINmeAIIIHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagctcgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaAjuISacIIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggtaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaNaeIPfoIPsiINgoMIVSV40 pAHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    tacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatcatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaatagBsmIBstZ17ISV40 pA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaa5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttNspIPciISapIBspQIpUC ori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggataccttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    tgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacaacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtpUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaAlwNIpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctaAhdIAmp (bla)6,3206,3406,3606,3806,4006,4206,4406,460
    acgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcatBsaINmeAIIIAmp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    agtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgtcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcAclIFspIPvuIAmp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgcAmp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcacctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtAclIXmnIAmp (bla)7,0207,0407,0607,0807,1007,1207,1407,160
    aaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaSspIbla promoterAmp (bla)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttAlwNISgrDI7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWI7,5407,5607,5807,600
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7602 bp550060006500700050010001500CCR6homologCMVTetR binding sitesTetO760-3186EKbF (DNA)XZp031.2 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA)EK_CMVFa (DNA),EK_CMVFb (DNA),EK_CMVFc (DNA),EK_CMVFd (DNA),EKo272.1 (DNA),SpeI,+1CMV promoter (with TetOs),A,EK_CMVFe (DNA),SnaBI,CspCITATA box,TetO,CMV-Forward Genewiz (DNA),Eco53kI,SacI,BsaBI,BsaBI,+3homolog,Kozak,startt,Signal/Flag,CMVF1 (DNA),ToFlpF (DNA),+5XZp031.1 (DNA),BspDI,ClaIEcoNI,SbfIPsiI,MscICCR6-seq-F1 (DNA)BsaIAvrIIAloIEcoNICCR6-seq-F2 (DNA)HindIIIBstBIFspIXZp031.3 (DNA)pUC oriAlwNIAhdIBsaINmeAIIIFspI,AclIAmp (bla),PvuIAclI,XmnISspIbla promoter,FlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo152.4 (DNA),+6

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