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XZ054 (PB)SFFV-LIDARsensor (hygR)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttaBspHIAmp20406080100120140160
    gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcccgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggBsaIBsrDIAmp180200220240260280300320340
    agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgAclIFspIBsrDIAmp360380400420440460480500520
    tccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacAmp540560580600620640660680700
    tgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctagAclIBcgIXmnIAmp720740760780800820840860880
    cagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcAmp9009209409609801,0001,0201,0401,060
    ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccBspHICspCI1,0801,1001,1201,1401,1601,1801,2001,2201,240
    caaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgagggttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccAloIPsiI1,2601,2801,3001,3201,3401,3601,3801,4001,420
    tgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgcacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcg1,4401,4601,4801,5001,5201,5401,5601,5801,600
    cgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcFspI1,6201,6401,6601,6801,7001,7201,7401,7601,780
    cgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattgtgacgtacgttaaagataatcatgtgtaaaatgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacactgcatgcaatttctattagtacacattttaBsiWI31bp spacer5'TR(1)BII(R)5'TR1,8001,8201,8401,8601,8801,9001,9201,940
    tgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacaaaaactcaaaatttcttctataaagtaacaaaacactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgtttttgagttttaaagaagatatttcattgttttg5'TR1,9601,9802,0002,0202,0402,0602,0802,1002,120
    ttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcgaaaPasISrfIcore insul2,1402,1602,1802,2002,2202,2402,2602,2802,300
    cctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaagctagctgcagtaacgccattatggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaataccgttcgatcgacgtcattgcggtaataBmtIMfeINheISfiISFFVcore insul2,3202,3402,3602,3802,4002,4202,4402,4602,480
    tgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggcccgaggccaagagcagaacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggcgtcaaagccggggccgggctccggttctcgtctEcoRVSFFV2,5002,5202,5402,5602,5802,6002,6202,6402,660
    tggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttctgcttcccgagctctataaaagagctcacaacccctcaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagacgaagggctcgagatattttctcgagtgttggggagAflIIBbvCIBpu10ISFFV2,6802,7002,7202,7402,7602,7802,8002,8202,840
    actcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGAscIMscIHindIIIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26NG28HE30FE32IE34GE36GE38GR40P2905-3612mCherrySFFVhomo...ch)2,8602,8802,9002,9202,9402,9602,9803,0003,020
    CTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84DY86LK88LS90FP92EG94FK96WE98RV1002905-3612mCherry3,0403,0603,0803,1003,1203,1403,1603,1803,200
    ATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCAloIBbsIKasINarISbfISfoIPflMIMN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT144MG146WE148AS150SE152RM154YP156ED158G2905-3612mCherry3,2203,2403,2603,2803,3003,3203,3403,3603,380
    CCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTBsgIKasINarISfoIBbvCIPluTIPluTIBpu10IA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK202LD204IT206SH208NE210DY212TI214VE216QY218E2905-3612mCherry3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAKpnIMluIXcmIZraIAatIIBamHIBsrGISgrAIAcc65IAflIIID1A2L3S1V2E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P333622-3720R220AE222GR224HS226TG228GM230DE232LY234KS2362905-3612G1T2G1S25' StopGo (2A; Loughran 2017)mCherry3,5803,6003,6203,6403,6603,6803,7003,720
    TAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAgeIBaeISpeIT1S2T1G2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T373733-3843E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L203856-3954ADAR substrate3xMS2 SGR3' StopGo (2A; Loughran 2017)3' stem5' stem3,7403,7603,7803,8003,8203,8403,8603,8803,900
    TCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACGTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCACAGAGTTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTPfoITliIXhoIBfuAIBspMIPaqCIPspXIPaeR7IL21K22L23A24G25D26V27E28S29N30P31G32P333856-3954S1S2VS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VS30GE32GE34GD36AT38YG40KL42TL44K3961-4674GFP3' StopGo (2A; Loughran 2017)3,9203,9403,9603,9804,0004,0204,0404,0604,080
    GTTCATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACAGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGACAAGTATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTBarIBstXISnaBIF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98FF100KD102DG1043961-4674GFP4,1004,1204,1404,1604,1804,2004,2204,2404,260
    AACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGAACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGCCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTAATTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTNY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144YN146SH148NV150YI152MA154DK156QK158NG160IK162V3961-4674GFP4,2804,3004,3204,3404,3604,3804,4004,4204,440
    ACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGCTTGCGGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGACTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200LS202TQ204SA206LS208KD210PN212EK214RD216HM218VL220LE222F3961-4674GFP4,4604,4804,5004,5204,5404,5604,5804,6004,620
    CGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACTGTATAAGtaggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGACATATTCatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcApaIBclIPmeISalITliIXhoIPspXIPaeR7IPspOMI*1V224TA226AG228IT230LG232MD234EL236YK2383961-4674GFPc-myc epitopeBGH polyad... sequencePolyhi...e taghomolog4,6404,6604,6804,7004,7204,7404,7604,7804,800
    ccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgBbsIBGH polyadenylation sequence4,8204,8404,8604,8804,9004,9204,9404,9604,980
    atgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcNotIXmnISexAIpSV405, 299 FBG...e5,0005,0205,0405,0605,0805,1005,1205,1405,160
    catgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaapSV405,1805,2005,2205,2405,2605,2805,3005,3205,340
    ggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgcttcctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcactactttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaBclIPmlIZraIAatIIAvrIIPshAIMK2KP4EL6TA8TS10VE12KF14LI16EK18FD20SV22SD24LM26QL28SE30GE32ES34RA365410-6435HygropSV405,3605,3805,4005,4205,4405,4605,4805,500
    tcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtEcoRIFS38FD40VG42GR44GY46VL48RV50NS52CA54DG56FY58KD60RY62VY64RH66FA68SA70AL72PI74PE76VL78DI80GE82FS84ES86LT88YC90IS92RR94AQ965410-6435Hygro2, 563 F5,5205,5405,5605,5805,6005,6205,6405,6605,680
    gggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgaAsiSIBfuAIBspMIGV98TL100QD102LP104ET106EL108PA110VL112QP114VA116EA118MD120AI122AA124AD126LS128QT130SG132FG134PF136GP138QG140IG142QY144TT146WR148DF150IC152AI154A5410-6435Hygro5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtD156PH158VY160HW162QT164VM166DD168TV170SA172SV174AQ176AL178DE180LM182LW184AE186DC188PE190VR192HL194VH196AD198FG200SN202NV204LT206DN208GR210IT212AV2145410-6435Hygro5,8805,9005,9205,9405,9605,9806,0006,0206,040
    ttgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgaAjuISacIIID216WS218EA220MF222GD224SQ226YE228VA230NI232FF234WR236PW238LA240CM242EQ244QT246RY248FE250RR252HP254EL256AG258SP260RL262RA264YM266LR268IG270LD272QL2745410-6435Hygro3, 135 F6,0606,0806,1006,1206,1406,1606,1806,2006,220
    ctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccgatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggYQ276SL278VD280GN282FD284DA286AW288AQ290GR292CD294AI296VR298SG300AG302TV304GR306TQ308IA310RR312SA314AV316WT318DG320CV322EV324LA326DS328GN330RR332P5410-6435Hygro6,2406,2606,2806,3006,3206,3406,3606,3806,400
    agcactcgtccgagggcaaaggaataggtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttactcgtgagcaggctcccgtttccttatccagctgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgHpaIPsiISalIS334TR336PR338AK340E*3425410-6435WPRESV40 pAHygroSV40 pA(1)6, 730 R6,4206,4406,4606,4806,5006,5206,5406,5606,580
    aaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaBsaIBsmISV40 pASV40 pA(1)6,6006,6206,6406,6606,6806,7006,7206,7406,760
    taatatttaaacagcagtgttcccaaactgggttcttatatcccttgctctggtcaaccaggttgcagggtttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttcccattataaatttgtcgtcacaagggtttgacccaagaatatagggaacgagaccagttggtccaacgtcccaaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaagggApaIBstXIEcoNISexAIPspOMI6,7806,8006,8206,8406,8606,8806,9006,9206,940
    cgtatccccccaggtgtctgcaggctcaaagagcagcgagaagcgttcagaggaaagcgatcccgtgccaccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcggggggcataggggggtccacagacgtccgagtttctcgtcgctcttcgcaagtctcctttcgctagggcacggtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgcccccSrfIcore insul(1) Geneious name: core insul(1)6,9606,9807,0007,0207,0407,0607,0807,1007,120
    agggacgtaattacatccctgggggctttgggggggggctgtccctgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgtccctgcattaatgtagggacccccgaaacccccccccgacagggactatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcPasIEcoRVcore insul(1) Geneio...name: core insul(1)7,1407,1607,1807,2007,2207,2407,2607,280
    cggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggtgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaBsmI3'TR7,3007,3207,3407,3607,3807,4007,4207,4407,460
    taatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgc7,4807,5007,5207,5407,5607,5807,6007,6207,640
    cacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggMscI7,6607,6807,7007,7207,7407,7607,7807,8007,820
    ggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggBsgIpLac(F)7,8407,8607,8807,9007,9207,9407,9607,9808,000
    acacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgaga8,0208,0408,0608,0808,1008,1208,1408,1608,180
    tccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaPciISapIBspQIAflIII8,2008,2208,2408,2608,2808,3008,3208,3408,360
    ttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagccc8,3808,4008,4208,4408,4608,4808,5008,5208,540
    aagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcgg8,5608,5808,6008,6208,6408,6608,6808,7008,720
    actggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtatgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccat8,7408,7608,7808,8008,8208,8408,8608,8808,900
    gcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagtt8,9208,940
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8956 bp65007000750080008500500100015002000core insul5'TR(1)AmppLac(F)EKo152.2 (DNA)EKo138.2 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)BspHI,BsaI,BsrDIFspI,AclI,BsrDIBcgIAclI,XmnIBspHICspCIPsiIAloIFrtF (DNA)FlpInR (DNA)FspIM13-40FOR Genewiz (DNA),M13F Genewiz (DNA)BII(R)5'TRBsiWI31bp spacer,5'TR,5'TRPasIBsaISexAIEWXglobF NONSPECIFIC DONT USE (DNA),EcoNI,PspOMI,+2core insul(1) Geneious name: core insul(1),SrfIPasIEcoRVBsmI3'TR,ES003-3 C->AMscIBsgIM13R Genewiz (DNA)pUCori5F (DNA),BspQI,SapI,AflIII,PciIpUCoriR (DNA)

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    8956