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YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaacccgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggEcoRIBstAPIDraIIIHygromycin20406080100120140160
    gaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacNdeIPvuIAsiSIRsrIIHygromycin180200220240260280300320340
    tgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagccBstAPIDraIIINmeAIIIHygromycin360380400420440460480500520
    ggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgAjuIBtgZISacIIHygromycin540560580600620640660680700
    aatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaattaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgttScaIHygromycin720740760780800820840860
    aggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagtccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcNaeIPfoIPsiIBsaAINgoMIVSV40 pA8809009209409609801,0001,0201,040
    caatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaBsmIBstZ17ISV40 pA1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccg1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaaPciISapIBspQIpUC ori1,4201,4401,4601,4801,5001,5201,5401,560
    gctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttcgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaapUC ori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcgagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcgpUC ori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaagtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgttpUC ori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    acaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagtgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatcpUC ori2,1202,1402,1602,1802,2002,2202,2402,260
    atccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaAhdIAmp (bla)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    taccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggBsaINmeAIIIAmp (bla)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagttcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcaAclIFspIPvuIAmp (bla)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    aagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgttcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcBcgIScaIAmp (bla)2,8202,8402,8602,8802,9002,9202,9402,960
    cgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttcAclIXmnIAmp (bla)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaacgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttSspIbla promoterAmp (bla)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctZraIAatIIBglIISgrDI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    caattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacatgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaagatggcccatcccctccgcgaaaagggttccgtcagacctcgtacgcgaaatcgtcggggcgacccgtgaaccgcgatgtgttcaccggagaccggagcgtgtgtaMfeIMluISphIBsiWIPGK3,5203,5403,5603,5803,6003,6203,6403,660
    tccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcattagtctcgtgcagatggacagcaccgctgaggtgtaggtggccatccgcggttggccgaggcaagaaaccaccggggaagcgcggtggaagatgaggaggggatcagtccttcaagggggggcggggcgtcgagcgcagcacgtcctgcactgtttaccttcatcgtgcagagtaatcagagcacgtctacctgtcgtggcgacAgeIBlpIPGK3,6803,7003,7203,7403,7603,7803,8003,8203,840
    agcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaatcgttaccttcgcccatccggaaaccccgtcgccggttatcgtcgaaacgaggaagcgaaagacccgagtctccgacccttccccacccaggcccccgcccgagtccccgcccgagtccccgccccgcccgcgggcttccaggaggcctccgggccgtaagacgtgcgaagttttBsmIStuIPGK3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctgcaggactctagcgtttaaacttaagcttcgccaccatggcgctccctgtcaccgcactgcttcttccgctggcactgctgctgcacgctgcacggcctggatccATGGGAGTGCAGGTGGAcgcgtgcagacggcgcgacaagaggagaaggagtagaggcccggaaagctggacgtcctgagatcgcaaatttgaattcgaagcggtggtaccgcgagggacagtggcgtgacgaagaaggcgaccgtgacgacgacgtgcgacgtgccggacctaggTACCCTCACGTCCACCTPmeISbfIAflIIBamHIPaqCIHindIIIMG2VQ4VE64184-4507M1A2L3P4V5T6A7L8L9L10P11L12A13L14L15L16H17A18A19R20P214115-4177G1S2CD8α signal peptidePGKFKBPfrom AV1324,0404,0604,0804,1004,1204,1404,1604,1804,200
    AACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTTTGGTAGAGGGGTCCTCTGCCCGCGTGGAAGGGGTTCGCGCCGGTCTGGACGCACCACGTGATGTGGCCCTACGAACTTCTACCTTTCTTTAAACTAAGGAGGGCCCTGTCTTTGTTCGGGAAATTCAAATACGATCCGTTCGTCCTCCACTAGGCTCCGACCCTTCTTCCCCAASmaIXmaITspMITI8SP10GD12GR14TF16PK18RG20QT22CV24VH26YT28GM30LE32DG34KK36FD38SS40RD42RN44KP46FK48FM50LG52KQ54EV56IR58GW60EE62GV644184-4507FKBPfrom AV1324,2204,2404,2604,2804,3004,3204,3404,360
    GCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTGGAAggcgccgggacaggagggtctgggggtggcactggcggatctgCGGGTCTACTCACACCCAGTCTCTCGGTTTGACTGATATAGAGGTCTAATACGGATACCACGGTGACCCGTGGGTCCGTAGTAGGGTGGTGTACGGTGAGAGCAGAAGCTACACCTCGAAGATTTTGACCTTccgcggccctgtcctcccagacccccaccgtgaccgcctagacAQ66MS68VG70QR72AK74LT76IS78PD80YA82YG84AT86GH88PG90II92PP94HA96TL98VF100DV102EL104LK106LE1084184-4507G1A2G3T4G5G6S7G8G9G10T11G12G13S144508-4630FKBPfrom AV13245-ECLfrom XG 2054,3804,4004,4204,4404,4604,4804,5004,5204,540
    gaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacagtggctctttgtgttgaaaattcCGGGTCAGGCGGTTCCGGCAGCGGctcctctgatgttcctgctactgctgttgccgcctagcccccccagaccaccatcaccgccaagcccccctccatggccagaccagcaccaacgacctcaggacaaaacaataccgaacgatcactgtcaccgagaaacacaacttttaagGCCCAGTCCGCCAAGGCCGTCGCCAgeIKpnIPsrIAcc65IN1S2G3S4G5G6S7G8S9G104697-4798E1G15G16D17Y18K19D20D21D22D23N24G25G26S27G28G29S30G31G32S33G34G35S36G37G38G39T40G414508-4630L1V2V3V4A5G6V7L8F9C10Y11G12L13L14V15T16V17A18L19C20V214631-4693from XG 20545-ECLCD28-TMD14aa GS linkerGS+XTEN linker4,5604,5804,6004,6204,6404,6604,6804,7004,720
    GGCGGGTAGCGGGTCCAGTGGGTCAGAAACACCCGGCACGAGTGAGTCAGCAACGCCAGAGAGTgctagcGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGCCGCCCATCGCCCAGGTCACCCAGTCTTTGTGGGCCGTGCTCACTCAGTCGTTGCGGTCTCTCAcgatcgCGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACAfeIBmtINheIA11G12S13G14S15S16G17S18E19T20P21G22T23S24E25S26A27T28P29E30S31A32S33A344697-4798LH2LD4QT6PS8RQ10PI12PS14EG16LQ18LH20LP22QV24LA26DA28VS30RL32VL344799-6007hADAR2 cat domainGS+XTEN linker16aa XTEN linker14aa ...nker4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAACCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTBstEIIGK36FG38DL40TD42NF44SS46PH48AR50RK52VL54AG56VV58MT60TG62TD64VK66DA68KV70IS72VS74TG76TK78CI80NG82EY84MS86DR88GL90AL924799-6007hADAR2 cat domain4,9204,9404,9604,9805,0005,0205,0405,060
    ATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGBclIBsrGIND94CH96AE98II100SR102RS104LL106RF108LY110TQ112LE114LY116LN118NK120DD122QK124RS126IF128QK130SE132RG134GF136RL138KE140NV142QF144HL146YI148ST150S4799-6007hADAR2 cat domain5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTBcgIPfoIP152CG154DA156RI158FS160PH162EP164IL166EE168PA170DR172HP174NR176KA178RG180QL182RT184KI186ES188GQ190GT192IP194VR196SN198AS200IQ202AW204DG206VL208Q4799-6007hADAR2 cat domainhomologousDim...ce5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCACCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTBlpITliIXhoIBamHIPaeR7IG210ER212LL214TM216SC218SD220KI222AR224WN226VV228GI230QG232SL234LS236IF238VE240PI242YF244SS246II248LG250SL252YH254GD256HL258SR260AM262YQ264RI266S4799-6007hADAR2 cat domain5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGN268IE270DL272PP274LY276TL278NK280PL282LS284GI286SN288AE290AR292QP294GK296AP298NF300SV302NW304TV306GD308SA310IE312VI314NA316TT318GK320DE322LG324RA3264799-6007hADAR2 cat domain5,6205,6405,6605,6805,7005,7205,7405,760
    GTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCMluISR328LC330KH332AL334YC336RW338MR340VH342GK344VP346SH348LL350RS352KI354TK356PN358VY360HE362SK364LA366AK368EY370QA372AK374AR376LF378TA380FI382KA3844799-6007hADAR2 cat domain5,7805,8005,8205,8405,8605,8805,9005,9205,940
    GGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgcCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacgApaIBclIPmeITliIXhoIPspXIPaeR7IPspOMI*1GL386GA388WV390EK392PT394EQ396DQ398FS400LT402P4799-6007hADAR2 cat domainc-myc epitopePolyhi...e tagBGH...ce5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacBtgZIBGH polyadenylation sequence6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcClaISphIBglIIBspDIBGH polyad... sequenceBGH pA6,3206,3406,3606,3806,4006,4206,4406,460
    cgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgagcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactKflINaeINgoMIV6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaBarIMscIXbaIZraIAatIIBsaAIPshAIFRTHygromycin6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttcagcttcgatgtaggagggcgtggatatgtcctaagtcgaagctacatcctcccgcacctatacaggaHygromycin6,8406,860
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6860 bp500055006000650050010001500pUC oriDimerization sequenceT501A*New StopHygromycin6008-60104799-6007EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)FlpInF2 (DNA)EKo152.4 (DNA)YH038.1 (DNA)oLSM0264 (DNA)oLSM0275 (DNA)EKbF (DNA)EKo272.2 (DNA)YH029.1 (DNA)CCp080 Cpep-F (DNA)CCp027-AV373Kozak-f (DNA)p450_F (DNA)p450_F (DNA)pPYK008 (DNA)pPYK021 (DNA)EWo100.1 (DNA)EKo283.2 (DNA)pNSK174 (DNA)EKo237.1 (DNA)EKo271.3 (DNA)EKo309.1 (DNA)YH039.1 (DNA)EKo93.1 (DNA)XZp112.2 (DNA)EK-ECLF (DNA)r-cXTEN-adar (DNA)XZp017.4 (DNA)EKo455.2 (DNA)XZp018.4 (DNA)EKbR (DNA)pNSK053 (DNA)YH039.2 (DNA)pNK018 (DNA)EKo326.3 (DNA)EcoRI,DraIII,BstAPIAsiSIPvuI,RsrII,NdeINmeAIII,BstAPI,DraIIIBtgZIAjuISacIIHyg_F (DNA)ScaIBsaAIPfoINgoMIVNaeISV40PA_R (DNA),+2SV40 pABsmIBstZ17IM13R Genewiz (DNA)BspQISapI+1PciIBclI+2+1PfoI+1E488Q,BcgI+1+1homologous,+1BamHI,BlpIPaeR7ITliIXhoIMluIEKo330.5 (DNA),PaeR7I,PspXI,+4c-myc epitope,Polyhistidine tagcyaToFlpR (DNA),BGHR Genewiz (DNA),PmeI,+1BghR1 (DNA)BGH polyadenylation sequence,BtgZIbghF (DNA),SphI,BglII,BspDI,ClaIBGH pA,EKo293.3 (DNA)FlpInR (DNA)FrtF (DNA),NgoMIV,NaeI,KflI,BsaAIFRT,EKo299.3 (DNA),XbaI,MscI,BarI,ZraI,AatII,PshAI

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    6860

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