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YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttEcoRIBstAPIDraIIIHygromycin20406080100120140160
    ctgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatgggacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactaccNdeIPvuIAsiSIRsrIIHygromycin180200220240260280300320340
    acgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccatgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggtBtgZIBstAPIDraIIINmeAIIIHygromycin360380400420440460480500520
    atacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgattatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactaAjuISacIIHygromycin540560580600620640660680700
    gcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtaccgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgScaIBsaAIHygromycin720740760780800820840860880
    tacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaaNaeIPfoIPsiINgoMIVSV40 pA9009209409609801,0001,0201,0401,060
    cacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggcctBsmIBstZ17ISV40 pA1,0801,1001,1201,1401,1601,1801,2001,2201,240
    agcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgtcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgacSapIBspQI1,2601,2801,3001,3201,3401,3601,3801,4001,420
    actcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgccctgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcgggPciIpUC ori1,4401,4601,4801,5001,5201,5401,5601,5801,600
    ccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatgggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtapUC ori1,6201,6401,6601,6801,7001,7201,7401,7601,780
    agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtpUC ori1,8001,8201,8401,8601,8801,9001,9201,940
    gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacpUC ori1,9601,9802,0002,0202,0402,0602,0802,1002,120
    caagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaagttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagattpUC ori2,1402,1602,1802,2002,2202,2402,2602,2802,300
    agtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccactcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgAhdIBsaIAmp (bla)2,3202,3402,3602,3802,4002,4202,4402,4602,480
    gctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaAclIFspINmeAIIIAmp (bla)2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataatccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaPvuIAmp (bla)2,6802,7002,7202,7402,7602,7802,8002,8202,840
    tctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccAclIBcgIScaIXmnIAmp (bla)2,8602,8802,9002,9202,9402,9602,9803,0003,020
    ggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtaAmp (bla)3,0403,0603,0803,1003,1203,1403,1603,1803,200
    actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtSspIZraIAatIIBglIISgrDIbla promoter3,2203,2403,2603,2803,3003,3203,3403,3603,380
    gtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctaMfeIBsiWI3,4003,4203,4403,4603,4803,5003,5203,5403,560
    atacgcgttctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttcttatgcgcaagatggcccatcccctccgcgaaaagggttccgtcagacctcgtacgcgaaatcgtcggggcgacccgtgaaccgcgatgtgttcaccggagaccggagcgtgtgtaaggtgtaggtggccatccgcggttggccgaggcaagaaaccaccggggaagcgcggtggaagaAgeIMluISphIPGK3,5803,6003,6203,6403,6603,6803,7003,720
    actcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcattagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggtgaggaggggatcagtccttcaagggggggcggggcgtcgagcgcagcacgtcctgcactgtttaccttcatcgtgcagagtaatcagagcacgtctacctgtcgtggcgactcgttaccttcgcccatccggaaaccccgtcgccggttatcgtcgaaacgaggaagcgaaagacccBlpIStuIPGK3,7403,7603,7803,8003,8203,8403,8603,8803,900
    ctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctgcaggactctagcgttgagtctccgacccttccccacccaggcccccgcccgagtccccgcccgagtccccgccccgcccgcgggcttccaggaggcctccgggccgtaagacgtgcgaagttttcgcgtgcagacggcgcgacaagaggagaaggagtagaggcccggaaagctggacgtcctgagatcgcaaBsmISbfIPGK3,9203,9403,9603,9804,0004,0204,0404,0604,080
    taaacttaagcttcgccaccatggcgctccctgtcaccgcactgcttcttccgctggcactgctgctgcacgctgcacggcctggatccATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATatttgaattcgaagcggtggtaccgcgagggacagtggcgtgacgaagaaggcgaccgtgacgacgacgtgcgacgtgccggacctaggTACCCTCACGTCCACCTTTGGTAGAGGGGTCCTCTGCCCGCGTGGAAGGGGTTCGCGCCGGTCTGGACGCACCACGTGATGTGGCCCTAPmeIAflIIBamHIPaqCIHindIIIMG2VQ4VE6TI8SP10GD12GR14TF16PK18RG20QT22CV24VH26YT28GM304184-4507M1A2L3P4V5T6A7L8L9L10P11L12A13L14L15L16H17A18A19R20P214115-4177G1S2FKBPfrom AV132CD8α signal peptide4,1004,1204,1404,1604,1804,2004,2204,2404,260
    GCTTGAAGATGGAAAGAAATTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCACGAACTTCTACCTTTCTTTAAACTAAGGAGGGCCCTGTCTTTGTTCGGGAAATTCAAATACGATCCGTTCGTCCTCCACTAGGCTCCGACCCTTCTTCCCCAACGGGTCTACTCACACCCAGTCTCTCGGTTTGACTGATATAGAGGTCTAATACGGATACCACGGTGACCCGTGGGTSmaIXmaITspMILE32DG34KK36FD38SS40RD42RN44KP46FK48FM50LG52KQ54EV56IR58GW60EE62GV64AQ66MS68VG70QR72AK74LT76IS78PD80YA82YG84AT86GH88P4184-4507FKBPfrom AV1324,2804,3004,3204,3404,3604,3804,4004,4204,440
    GGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTGGAAggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccgCCGTAGTAGGGTGGTGTACGGTGAGAGCAGAAGCTACACCTCGAAGATTTTGACCTTccgcggccctgtcctcccagacccccaccgtgaccgcctagacctcctctgatgttcctgctactgctgttgccgcctagcccccccagaccaccatcaccgccaagcccccctccatggcAgeIKpnIPsrIAcc65IG90II92PP94HA96TL98VF100DV102EL104LK106LE1084184-4507G1A2G3T4G5G6S7G8G9G10T11G12G13S14G15G16D17Y18K19D20D21D22D23N24G25G26S27G28G29S30G31G32S33G34G35S36G37G38G39T404508-463045-ECLfrom XG 205FKBPfrom AV1324,4604,4804,5004,5204,5404,5604,5804,6004,620
    gtctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacagtggctctttgtgttgaaaattcCGGGTCAGGCGGTTCCGGCAGCGGGGCGGGTAGCGGGTCCAGTGGGTCAGAAACACCCGGCACGAGTGAGTCAGCAACGCCAGAGAGTgctagcGCTTTGCACTTcagaccagcaccaacgacctcaggacaaaacaataccgaacgatcactgtcaccgagaaacacaacttttaagGCCCAGTCCGCCAAGGCCGTCGCCCCGCCCATCGCCCAGGTCACCCAGTCTTTGTGGGCCGTGCTCACTCAGTCGTTGCGGTCTCTCAcgatcgCGAAACGTGAAAfeIBmtINheIN1S2G3S4G5G6S7G8S9G10A11G12S13G14S15S16G17S18E19T20P21G22T23S24E25S26A27T28P29E30S31A32S33A344697-4798LH2LE1G41L1V2V3V4A5G6V7L8F9C10Y11G12L13L14V15T16V17A18L19C20V214631-4693GS+XTEN linkerfrom XG 205CD28-TMD16aa XTEN linker14aa GS linker4,6404,6604,6804,7004,7204,7404,7604,7804,800
    GGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCCCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGBstEIID4QT6PS8RQ10PI12PS14EG16LQ18LH20LP22QV24LA26DA28VS30RL32VL34GK36FG38DL40TD42NF44SS46PH48AR50RK52VL54AG56VV58MT60TG624799-6007hADAR2 cat domain4,8204,8404,8604,8804,9004,9204,9404,9604,980
    ACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACBclITD64VK66DA68KV70IS72VS74TG76TK78CI80NG82EY84MS86DR88GL90AL92ND94CH96AE98II100SR102RS104LL106RF108LY110TQ112LE114LY116LN118NK120D4799-6007hADAR2 cat domain5,0005,0205,0405,0605,0805,1005,1205,1405,160
    ATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACATAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTPfoIBsrGID122QK124RS126IF128QK130SE132RG134GF136RL138KE140NV142QF144HL146YI148ST150SP152CG154DA156RI158FS160PH162EP164IL166EE168PA170DR172HP174NR176KA178RG180Q4799-6007hADAR2 cat domain5,1805,2005,2205,2405,2605,2805,3005,3205,340
    GCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCBcgIBlpIBamHIL182RT184KI186ES188GQ190GT192IP194VR196SN198AS200IQ202AW204DG206VL208QG210ER212LL214TM216SC218SD220KI222AR224WN226VV228GI230QG232SL234LS236IF238VE2404799-6007hADAR2 cat domainhomologousDim...ce5,3605,3805,4005,4205,4405,4605,4805,500
    CCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGATliIXhoIPaeR7IPI242YF244SS246II248LG250SL252YH254GD256HL258SR260AM262YQ264RI266SN268IE270DL272PP274LY276TL278NK280PL282LS284GI286SN288AE290AR292QP294GK296AP298N4799-6007hADAR2 cat domain5,5205,5405,5605,5805,6005,6205,6405,6605,680
    TCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCAMluIF300SV302NW304TV306GD308SA310IE312VI314NA316TT318GK320DE322LG324RA326SR328LC330KH332AL334YC336RW338MR340VH342GK344VP346SH348LL350RS352KI354TK356PN358V4799-6007hADAR2 cat domain5,7005,7205,7405,7605,7805,8005,8205,8405,860
    GTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagagCATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcApaITliIXhoIPspXIPaeR7IPspOMI*1Y360HE362SK364LA366AK368EY370QA372AK374AR376LF378TA380FI382KA384GL386GA388WV390EK392PT394EQ396DQ398FS400LT402P4799-6007hADAR2 cat domainc-myc epitope5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtaBclIPmeIBtgZIBGH polyadenylation sequencePolyhi...e tag6,0606,0806,1006,1206,1406,1606,1806,2006,220
    cgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgacClaISphIBglIIBspDIBGH polyadenylation sequenceBGH pA6,2406,2606,2806,3006,3206,3406,3606,3806,400
    gggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggcccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccKflINaeINgoMIV6,4206,4406,4606,4806,5006,5206,5406,5606,580
    tccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactaBarIMscIXbaIZraIAatIIBsaAIPshAIFRTHygromycin6,6006,6206,6406,6606,6806,7006,7206,7406,760
    cgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggaHygromycin6,7806,8006,8206,8406,860
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6860 bp500055006000650050010001500pUC oriDimerization sequenceT501A*New StopHygromycin6008-60104799-6007EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)FlpInF2 (DNA)EKo152.4 (DNA)YH038.1 (DNA)oLSM0264 (DNA)EKbF (DNA)oLSM0275 (DNA)EKo272.2 (DNA)CCp080 Cpep-F (DNA)CCp027-AV373Kozak-f (DNA)YH029.1 (DNA)p450_F (DNA)p450_F (DNA)pPYK021 (DNA)pPYK008 (DNA)EKo283.2 (DNA)EWo100.1 (DNA)pNSK174 (DNA)EKo237.1 (DNA)EKo271.3 (DNA)EKo309.1 (DNA)YH039.1 (DNA)EKo93.1 (DNA)XZp112.2 (DNA)EK-ECLF (DNA)r-cXTEN-adar (DNA)XZp017.4 (DNA)EKo455.2 (DNA)XZp018.4 (DNA)EKbR (DNA)pNSK053 (DNA)YH039.2 (DNA)pNK018 (DNA)EKo326.3 (DNA)EcoRI,DraIII,BstAPIAsiSIPvuI,RsrII,NdeINmeAIII,BstAPI,DraIIIBtgZIAjuISacIIHyg_F (DNA)ScaIBsaAIPfoINgoMIVNaeISV40PA_R (DNA),+2SV40 pABsmIBstZ17IM13R Genewiz (DNA)BspQISapI+1PciIBclI+2+1PfoI+1E488Q,BcgI+1+1homologous,+1BamHI,BlpIPaeR7ITliIXhoIMluIEKo330.5 (DNA),PaeR7I,PspXI,TliI,+3c-myc epitope,Polyhistidine tagcyaToFlpR (DNA),BGHR Genewiz (DNA),PmeI,BclIBghR1 (DNA)BGH polyadenylation sequence,BtgZIbghF (DNA),SphI,BglII,BspDI,ClaIBGH pA,EKo293.3 (DNA)FlpInR (DNA)FrtF (DNA),NgoMIV,NaeI,KflI,BsaAIFRT,EKo299.3 (DNA),XbaI,MscI,BarI,ZraI,AatII,PshAI

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    6860