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LSM044-2 CMV-TO-CCR7-V2-ADAR2dd(E488Q, T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttapUC ori20406080100120140160
    gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcccgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggAhdIAmp (bla)180200220240260280300320340
    agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgAclIFspINmeAIIIAmp (bla)360380400420440460480500520
    tccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacPvuIAmp (bla)540560580600620640660680700
    tgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctagAclIBcgIScaIXmnIAmp (bla)720740760780800820840860880
    cagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcSspIAmp (bla)bla promoter9009209409609801,0001,0201,0401,060
    ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtaccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatSgrDIbla promoter1,0801,1001,1201,1401,1601,1801,2001,2201,240
    tctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacattgattattgactagttattaataagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtaactaataactgatcaataattatMfeIMluISpeIAlwNIBsiWIBpu10ICMVCMV promoter (with TetOs)1,2601,2801,3001,3201,3401,3601,3801,4001,420
    gtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcCMVCMV promoter (with TetOs)1,4401,4601,4801,5001,5201,5401,5601,5801,600
    tatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgaataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactNdeISnaBICMVCMV promoter (with TetOs)1,6201,6401,6601,6801,7001,7201,7401,7601,780
    tgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccacCspCICMVCMV promoter (with TetOs)1,8001,8201,8401,8601,8801,9001,9201,940
    ggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttSacISacIBsaBIBsaBIBsmBIEsp3ISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetOhomologfrom...me)1,9601,9802,0002,0202,0402,0602,0802,1002,120
    cttaagcttcgccaccATGGATCTCGGGAAGCCTATGAAAAGCGTACTGGTTGTGGCTTTGCTGGTGATATTCCAGGTATGTCTGTGTCAGGATGAAGTTACAGACGACTACATAGGCGACAATACCACAGTAGATTACACCCTTTTCGAGTCCCTCTGCAGCAAGAAGGACGTGAGGgaattcgaagcggtggTACCTAGAGCCCTTCGGATACTTTTCGCATGACCAACACCGAAACGACCACTATAAGGTCCATACAGACACAGTCCTACTTCAATGTCTGCTGATGTATCCGCTGTTATGGTGTCATCTAATGTGGGAAAAGCTCAGGGAGACGTCGTTCTTCCTGCACTCCAflIIBmgBIHindIIIMD2LG4KP6MK8SV10LV12VA14LL16VI18FQ20VC22LC24QD26EV28TD30DY32IG34DN36TT38VD40YT42LF44ES46LC48SK50KD52VR542153-4597from original LSM044 (digest same)Ccr72,1402,1602,1802,2002,2202,2402,2602,2802,300
    AATTTCAAGGCATGGTTTCTGCCTATTATGTATTCCATAATCTGCTTTGTCGGGCTGCTGGGGAACGGTTTGGTCGTCTTGACGTATATTTACTTCAAACGCCTGAAGACAATGACAGATACCTACCTTTTGAACCTCGCCGTGGCCGATATTCTGTTTCTTCTCACTCTCCCTTTCTTTAAAGTTCCGTACCAAAGACGGATAATACATAAGGTATTAGACGAAACAGCCCGACGACCCCTTGCCAAACCAGCAGAACTGCATATAAATGAAGTTTGCGGACTTCTGTTACTGTCTATGGATGGAAAACTTGGAGCGGCACCGGCTATAAGACAAAGAAGAGTGAGAGGGAAAGABarINF56KA58WF60LP62IM64YS66II68CF70VG72LL74GN76GL78VV80LT82YI84YF86KR88LK90TM92TD94TY96LL98NL100AV102AD104IL106FL108LT110LP112F2153-4597from original LSM044 (digest same)Ccr7TM12,3202,3402,3602,3802,4002,4202,4402,4602,480
    GGGCATATTCCGCAGCAAAGTCATGGGTCTTCGGAGTCCACTTTTGCAAGCTGATCTTTGCTATATACAAGATGAGCTTTTTCTCTGGGATGCTGCTGCTGCTGTGCATCAGCATAGATAGATATGTGGCCATTGTGCAGGCGGTCTCTGCACATAGACACCGGGCTAGGGTGCTTCTCCCGTATAAGGCGTCGTTTCAGTACCCAGAAGCCTCAGGTGAAAACGTTCGACTAGAAACGATATATGTTCTACTCGAAAAAGAGACCCTACGACGACGACGACACGTAGTCGTATCTATCTATACACCGGTAACACGTCCGCCAGAGACGTGTATCTGTGGCCCGATCCCACGAAGAW114AY116SA118AK120SW122VF124GV126HF128CK130LI132FA134IY136KM138SF140FS142GM144LL146LL148CI150SI152DR154YV156AI158VQ160AV162SA164HR166HR168AR170VL172L2153-4597from original LSM044 (digest same)Ccr72,5002,5202,5402,5602,5802,6002,6202,6402,660
    GATAAGTAAATTGAGCTGCGTTGGGATCTGGATTTTGGCCACAGTCTTGTCCATTCCTGAACTGTTGTACTCAGATTTGCAGAGGAGTTCCTCTGAGCAAGCTATGCGATGTTCACTGATCACTGAGCATGTGGAAGCGTTCATTACGATCCAAGTCGCCCAAATGGTGATTGGCTTCCTATTCATTTAACTCGACGCAACCCTAGACCTAAAACCGGTGTCAGAACAGGTAAGGACTTGACAACATGAGTCTAAACGTCTCCTCAAGGAGACTCGTTCGATACGCTACAAGTGACTAGTGACTCGTACACCTTCGCAAGTAATGCTAGGTTCAGCGGGTTTACCACTAACCGAAGI174SK176LS178CV180GI182WI184LA186TV188LS190IP192EL194LY196SD198LQ200RS202SS204EQ206AM208RC210SL212IT214EH216VE218AF220IT222IQ224VA226QM228VI230GF2322153-4597from original LSM044 (digest same)Ccr72,6802,7002,7202,7402,7602,7802,8002,8202,840
    CTTGTTCCTTTGCTTGCTATGAGCTTTTGCTACCTCGTAATTATACGCACACTCCTGCAGGCCCGGAATTTTGAGCGGAACAAGGCTATTAAGGTGATAATTGCCGTAGTGGTTGTATTCATTGTCTTTCAGCTGCCTTATAATGGGGTAGTTTTGGCCCAAACAGTTGCTAACTTTAGAACAAGGAAACGAACGATACTCGAAAACGATGGAGCATTAATATGCGTGTGAGGACGTCCGGGCCTTAAAACTCGCCTTGTTCCGATAATTCCACTATTAACGGCATCACCAACATAAGTAACAGAAAGTCGACGGAATATTACCCCATCAAAACCGGGTTTGTCAACGATTGAAATPsiISbfILV234PL236LA238MS240FC242YL244VI246IR248TL250LQ252AR254NF256ER258NK260AI262KV264II266AV268VV270VF272IV274FQ276LP278YN280GV282VL284AQ286TV288AN290F2153-4597from original LSM044 (digest same)Ccr72,8602,8802,9002,9202,9402,9602,9803,0003,020
    ACATCACTTCTTCAACATGTGAGCTGTCAAAGCAGCTGAATATCGCATACGATGTGACATACTCCTTGGCGTGCGTCAGGTGTTGCGTGAATCCTTTCCTGTACGCTTTTATTGGTGTGAAGTTTCGAAATGACCTGTTTAAACTTTTCAAAGACTTGGGCTGTCTGTCTCAGGAGCATGTAGTGAAGAAGTTGTACACTCGACAGTTTCGTCGACTTATAGCGTATGCTACACTGTATGAGGAACCGCACGCAGTCCACAACGCACTTAGGAAAGGACATGCGAAAATAACCACACTTCAAAGCTTTACTGGACAAATTTGAAAAGTTTCTGAACCCGACAGACAGAGTCCTCGTPciIXmnIBstBIN292IT294SS296TC298EL300SK302QL304NI306AY308DV310TY312SL314AC316VR318CC320VN322PF324LY326AF328IG330VK332FR334ND336LF338KL340FK342DL344GC346LS348QE350Q2153-4597from original LSM044 (digest same)Ccr73,0403,0603,0803,1003,1203,1403,1603,1803,200
    GCTTCGCCAGTGGTCATCATGCAGACACATTAGGCGCTCCAGTATGAGTGTGGAGGCAGAGACCACTACTACGTTCTCCCCTaccggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTCGAAGCGGTCACCAGTAGTACGTCTGTGTAATCCGCGAGGTCATACTCACACCTCCGTCTCTGGTGATGATGCAAGAGGGGAtggccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCAAgeIAgeIKflIKpnIEcoNIAcc65IL352RQ354WS356SC358RH360IR362RS364SM366SV368EA370ET372TT374TF376SP378TG380GR382TP384PS386LG388PQ390DE392SC394TT396AS398SS400LA402KD404TS406SG408TG4102153-4597from original LSM044 (digest same)Ccr7V2 tail3,2203,2403,2603,2803,3003,3203,3403,3603,380
    GCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTBstEIIAL412HL414DQ416TP418SR420QP422IP424SE426GL428QL430HL432PQ434VL436AD438AV440SR442LV444LG446KF448GD450LT452DN454FS456SP458HA460RR462KV464LA466GV468V2153-4597hADAR2 cat domainfrom ...ame)3,4003,4203,4403,4603,4803,5003,5203,5403,560
    TGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAAACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTM470TT472GT474DV476KD478AK480VI482SV484ST486GT488KC490IN492GE494YM496SD498RG500LA502LN504DC506HA508EI510IS512RR514SL516LR518FL520YT522QL524EL526YL528N2153-4597hADAR2 cat domain3,5803,6003,6203,6403,6603,6803,7003,720
    TAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTPfoIBsrGIN530KD532DQ534KR536SI538FQ540KS542ER544GG546FR548LK550EN552VQ554FH556LY558IS560TS562PC564GD566AR568IF570SP572HE574PI576LE578EP580AD582RH584PN586RK5882153-4597hADAR2 cat domain3,7403,7603,7803,8003,8203,8403,8603,8803,900
    GCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCACGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTBcgIBlpIBamHIAR590GQ592LR594TK596IE598SG600QG602TI604PV606RS608NA610SI612QA614WD616GV618LQ620GE622RL624LT626MS628CS630DK632IA634RW636NV638VG640IQ642GS644LL646S2153-4597hADAR2 cat domain3,9203,9403,9603,9804,0004,0204,0404,0604,080
    TTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTTliIXhoIPaeR7II648FV650EP652IY654FS656SI658IL660GS662LY664HG666DH668LS670RA672MY674QR676IS678NI680ED682LP684PL686YT688LN690KP692LL694SG696IS698NA700EA702RQ704PG706K2153-4597hADAR2 cat domain4,1004,1204,1404,1604,1804,2004,2204,2404,260
    GGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGMluIA708PN710FS712VN714WT716VG718DS720AI722EV724IN726AT728TG730KD732EL734GR736AS738RL740CK742HA744LY746CR748WM750RV752HG754KV756PS758HL760LR762SK764IT7662153-4597hADAR2 cat domain4,2804,3004,3204,3404,3604,3804,4004,4204,440
    AAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcaTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtApaITliIXhoIPspXIBpu10IPaeR7IPspOMI*1KP768NV770YH772ES774KL776AA778KE780YQ782AA784KA786RL788FT790AF792IK794AG796LG798AW800VE802KP804TE806QD808QF810SL812TP814*2153-4597hADAR2 cat domainc-m...pe4,4604,4804,5004,5204,5404,5604,5804,6004,620
    tctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactBGH polyadenylation sequencec-myc epitopePolyhi...e tag4,6404,6604,6804,7004,7204,7404,7604,7804,800
    ggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaacctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgccttClaISphIBspDIBGH polyadenylation sequenceBGH pA4,8204,8404,8604,8804,9004,9204,9404,9604,980
    agaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagNaeINgoMIV5,0005,0205,0405,0605,0805,1005,1205,1405,160
    taaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcBarIKflIXbaIPshAIFRTHygromycin5,1805,2005,2205,2405,2605,2805,3005,3205,340
    aagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgBsmBIEsp3IHygromycin5,3605,3805,4005,4205,4405,4605,4805,500
    tcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcPvuIAsiSIEcoRIDraIIIHygromycin5,5205,5405,5605,5805,6005,6205,6405,6605,680
    cgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttNdeIRsrIIHygromycin5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagcAjuIDraIIINmeAIIIHygromycin5,8805,9005,9205,9405,9605,9806,0006,0206,040
    agcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaattcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttaSacIIHygromycin6,0606,0806,1006,1206,1406,1606,1806,2006,220
    cgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggcgcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgNaeIPfoIScaINgoMIVHygromycin6,2406,2606,2806,3006,3206,3406,3606,3806,400
    tggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacBsmIPsiISV40 pA6,4206,4406,4606,4806,5006,5206,5406,5606,580
    tctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgccagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacggBstZ17I6,6006,6206,6406,6606,6806,7006,7206,7406,760
    cgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccgcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggSapIBspQI6,7806,8006,8206,8406,8606,8806,9006,9206,940
    acagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacctgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggPciIpUC ori6,9606,9807,0007,0207,0407,0607,0807,1007,120
    aggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccctccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttgggggpUC ori7,1407,1607,1807,2007,2207,2407,2607,280
    cgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtAlwNIpUC ori7,3007,3207,3407,3607,3807,4007,4207,4407,460
    gtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttpUC ori7,4807,5007,5207,5407,5607,5807,6007,620
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7620 bp550060006500700050010001500HygromycinCMV promoter (with TetOs)CMVASV40 pAbla promoterAmp (bla)pUC oriFlpInF2 (DNA)YXo025.1 (DNA)XZp025.1 (DNA)oLSM0118 (DNA)EKbF (DNA)EKo272.2 (DNA)p450_F (DNA)oLSM0119 (DNA)XZp032.3 (DNA)pNSK053 (DNA)YXo033.4 (DNA)pNK018 (DNA)bghF (DNA)oLSM0086 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)AhdIEKo152.2 (DNA),NmeAIII,FspI,AclIPvuIScaIBcgIAclI,XmnIoLSM0054 (DNA),oLSM0053 (DNA)SspISgrDIFlpInF1 (DNA),AlwNIFlpInF (DNA)oLSM039 (DNA),EKo152.4 (DNA),EKo279.1 (DNA),MfeI,+3EKo272.1 (DNA),SpeIEK_CMVFa (DNA)EK_CMVFb (DNA)EK_CMVFc (DNA),NdeIEK_CMVFd (DNA)SnaBIEK_CMVFe (DNA)CspCICMV-Forward Genewiz (DNA),pAAV-F (DNA)NdeINmeAIIIDraIIIAjuISacIIHyg_F (DNA)ScaIPfoI,NgoMIV,NaeISV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiIBsmI,BstZ17IM13R Genewiz (DNA)BspQI,SapIpUCori5F (DNA)PciIAlwNI

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    7620