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pLenti-mThy1-hChR2(H134R)-EYFP-WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaacagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttMfeISgrDI20406080100120140160
    gaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgacttagacgaatcccaatccgcaaaacgcgacgaagcgctacatgcccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactMluISpeI180200220240260280300320340
    ccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatNdeI360380400420440460480500520
    aatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcaCspCISnaBI540560580600620640660680700
    caatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctcgttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtcgcgcaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagag5'LTR720740760780800820840860
    tggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctKasINarISfoIPluTI5'LTR8809009209409609801,0001,0201,040
    aaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaattggtctcctcgagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttBssHII1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagattaagccaattccggtccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatct1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtaatatattatgtcatcgttgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggcgactagaagtctggacctcctcctctatactccctgttaacctctMfeINotISapIBspQIEcoNI1,4201,4401,4601,4801,5001,5201,5401,560
    agtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtcacttaatatatttatatttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccRRE1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggatgtccggtctgttaataacagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccRRE1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaacgagaccttttgagtaaacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttc1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcacttacttgttcttaataaccttaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtg2,1202,1402,1602,1802,2002,2202,2402,260
    cattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaattttaaaagaagtaatagcaaagtctgggtggagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagccgtgacgcacgcggttaagacgtctgtttaccgtcataagtaggtgttaaaattttctt2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttggttaaagagaccgggaaccaaactagcctttaattcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctctaggtcaaaccaatttctctggcccttggtttgatcggaaattmThy12,4602,4802,5002,5202,5402,5602,5802,6002,620
    aaaacataagtacaggagccagcaagatggctcagtgggtaaaggtgcctaccagcaagcctgacagcctgagttcagtccccacgaactacgtggtaggagaggaccaaccaactctggaaatctgttctgcaaacacatgctcacacacacacacacaaatagtataaacaatttttgtattcatgtcctcggtcgttctaccgagtcacccatttccacggatggtcgttcggactgtcggactcaagtcaggggtgcttgatgcaccatcctctcctggttggttgagacctttagacaagacgtttgtgtacgagtgtgtgtgtgtgtgtttatcatatttgttaXcmImThy12,6402,6602,6802,7002,7202,7402,7602,7802,800
    tttaaatttcatttaaaaataatttgtaaacaaaatcattagcacaggttttagaaagagcctcttggtgacatcaagttgatgctgtagatggggtatcattcctgaggacccaaaaccgggtctcagcctttccccattctgagagttctctcttttctcagccactagctgaaaatttaaagtaaatttttattaaacatttgttttagtaatcgtgtccaaaatctttctcggagaaccactgtagttcaactacgacatctaccccatagtaaggactcctgggttttggcccagagtcggaaaggggtaagactctcaagagagaaaagagtcggtgatcgactmThy12,8202,8402,8602,8802,9002,9202,9402,960
    agagtagagtggctcagcactgggctcttgagttcccaagtcctacaactggtcagcctgactactaaccagccatgaagaaacaaggagtggatgggctgagtctgctgggatgggagtggagttagtaagtggccatggatgtaatgaccccagcaatgctggctagaaggcatctcatctcaccgagtcgtgacccgagaactcaagggttcaggatgttgaccagtcggactgatgattggtcggtacttctttgttcctcacctacccgactcagacgaccctaccctcacctcaatcattcaccggtacctacattactggggtcgttacgaccgatcttccgtBlpImThy12,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgcctcctttccttgtctggagacggaacgggatcatcttgtactcacagaagggagaacattctagctggttgggccaaaatgtgcaagttcacctggaggtggtggtgcatgcttttaactccagtactcaggaggcagggccaggtggatctctgtgagttcaagaccagccacggaggaaaggaacagacctctgccttgccctagtagaacatgagtgtcttccctcttgtaagatcgaccaacccggttttacacgttcaagtggacctccaccaccacgtacgaaaattgaggtcatgagtcctccgtcccggtccacctagagacactcaagttctggtcggScaIBsmBIEsp3ImThy13,1603,1803,2003,2203,2403,2603,2803,3003,320
    tgcactatggagagagttttgggacagccagagttacacagaaaaatcctggtggaaaatctgaaagaaagagagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaaggaagaaagaaagaaagagtggcaggcaggcaggcagacgtgatacctctctcaaaaccctgtcggtctcaatgtgtctttttaggaccaccttttagactttctttctctctttctttctttctttctttctttctttctttctttctttctttctttctttctttctttctttccttctttctttctttctcaccgtccgtccgtccgtcmThy13,3403,3603,3803,4003,4203,4403,4603,4803,500
    gaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaaataggtgcgacttcaagatccggagttacaagcagaatgcactgtttccctaacagggccaagtgttttgagtaactgaaggtgggcatgatgcctgggaagcagaaactccttccttccttccttccttccttccttccttccttccttccttccttccttccttccttccttttatccacgctgaagttctaggcctcaatgttcgtcttacgtgacaaagggattgtcccggttcacaaaactcattgacttccacccgtactacggacccttcgtctttmThy13,5203,5403,5603,5803,6003,6203,6403,660
    caagccaggcagatgcaccccttgccttgcttccgaagggctgcagtagcatggaaaacatggaaaacaaccaatccattccctttgctgatataacaggctccaaagccaaaacctgtcactggaggctcaagagcagatctccagccaagaggcaaaggaatgggggaagctggttcggtccgtctacgtggggaacggaacgaaggcttcccgacgtcatcgtaccttttgtaccttttgttggttaggtaagggaaacgactatattgtccgaggtttcggttttggacagtgacctccgagttctcgtctagaggtcggttctccgtttccttacccccttcgacmThy13,6803,7003,7203,7403,7603,7803,8003,8203,840
    gagggcctccctctggttatccaggcttctgaaggttcaagcaaagaaagggttacaaccttaaaaggagagcgtcccggggtatgggtagaagactgctccaccccgacccccagggtccctaaccgtcttttccctgggcgagtcagcccaatcacaggactgagagtgcctcctcccggagggagaccaataggtccgaagacttccaagttcgtttctttcccaatgttggaattttcctctcgcagggccccatacccatcttctgacgaggtggggctgggggtcccagggattggcagaaaagggacccgctcagtcgggttagtgtcctgactctcacggagPasIPasImThy13,8603,8803,9003,9203,9403,9603,9804,0004,020
    tttagtagcagcaagccacttcggacacccaaatggaacacctccagtcagccctcgccgaccaccccaccccctccatccttttccctcagcctccgattggctgaatctagagtccctccctgctcccccctctctccccacccctggtgaaaactgcgggcttcagcgctggaaatcatcgtcgttcggtgaagcctgtgggtttaccttgtggaggtcagtcgggagcggctggtggggtgggggaggtaggaaaagggagtcggaggctaaccgacttagatctcagggagggacgaggggggagagaggggtggggaccacttttgacgcccgaagtcgcgaccAfeIXbaImThy14,0404,0604,0804,1004,1204,1404,1604,1804,200
    gtgcagcaactggaggcgttggcgcaccaggaggaggctgcagctaggggagtccaggtgagagcaggccgacgggagggacccgcacatgcaaggaccgccgcagggcgaggatgcaagccttccccagctacagttttgggaaaggataccagggcgctcctatatgggggcgcacgtcgttgacctccgcaaccgcgtggtcctcctccgacgtcgatcccctcaggtccactctcgtccggctgccctccctgggcgtgtacgttcctggcggcgtcccgctcctacgttcggaaggggtcgatgtcaaaaccctttcctatggtcccgcgaggatatacccccgcmThy14,2204,2404,2604,2804,3004,3204,3404,360
    cgggaactggggaaagaaggtgctcccaggtcgaggtgggagaggaaggcagtgcggggtcacgggctttctccctgctaacggacgctttcgaagagtgggtgccggaggagaaccatgaggaaggacatcaaggacagcctttggtccccaagctcaaatcgctttagtggtggcccttgacccctttcttccacgagggtccagctccaccctctccttccgtcacgccccagtgcccgaaagagggacgattgcctgcgaaagcttctcacccacggcctcctcttggtactccttcctgtagttcctgtcggaaaccaggggttcgagtttagcgaaatcaccacBstBImThy14,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgaatagagggaggaggtgggtggcaaactggagggagtccccagcgggtgacctcgtggctggctgggtgcggggcaccgcaggtaagaaaaccgcaatgttgcgggaggggactgggtggcaggcgcgggggaggggaaagctagaaaggatgcgagggagcggaggggggaggcttatctccctcctccacccaccgtttgacctccctcaggggtcgcccactggagcaccgaccgacccacgccccgtggcgtccattcttttggcgttacaacgccctcccctgacccaccgtccgcgccccctcccctttcgatctttcctacgctccctcgcctcccccctcBfuAIBspMIBstEIImThy14,5604,5804,6004,6204,6404,6604,6804,7004,720
    ggagcgggagaatctcaactggtagaggaagattaaaatgaggaaatagcatcagggtggggttagccaagccgggcctcagggaaagggcgcaaagtttgtctgggtgtgggcttaggtgggctgggtatgagattcggggcgccgaaaacactgctgcgcctctgccaaatcacctcgccctcttagagttgaccatctccttctaattttactcctttatcgtagtcccaccccaatcggttcggcccggagtccctttcccgcgtttcaaacagacccacacccgaatccacccgacccatactctaagccccgcggcttttgtgacgacgcggagacggtttagtBaeIKasINarISfoIPluTImThy14,7404,7604,7804,8004,8204,8404,8604,8804,900
    cgctacccctgtatctagttctgccaggcttctccagccccagccccaattcttttctctagtgttcccccttccctcccctgaatctcaagcccacactccctcctccataacccactgttatcaaatccaagtcatttgccacccaacaaccatcaggaggcggaagcagacggcgatggggacatagatcaagacggtccgaagaggtcggggtcggggttaagaaaagagatcacaagggggaagggaggggacttagagttcgggtgtgagggaggaggtattgggtgacaatagtttaggttcagtaaacggtgggttgttggtagtcctccgccttcgtctgcmThy14,9204,9404,9604,9805,0005,0205,0405,060
    ggaggagtttgagatcaacttgggctacatcacgagttccaggctcaccaaggcttcttaaggagaccttgtctctaaaattaattaattaattaattaatagtcccctttctctgccacagaaccttgggatctggctcctggtcgcagctccccccaccccaggctgacattccctcctcaaactctagttgaacccgatgtagtgctcaaggtccgagtggttccgaagaattcctctggaacagagattttaattaattaattaattaattatcaggggaaagagacggtgtcttggaaccctagaccgaggaccagcgtcgaggggggtggggtccgactgtaagPflFITth111ImThy15,0805,1005,1205,1405,1605,1805,2005,2205,240
    actgccatagcccatccggaaatcctagtctatttccccatggatcttgaactgcagagagaatggcagagtggcccgccctgtgcaaaggatgttcctagcctaggtggagctcgcgaactcgcagactgtgcctctcttgggcaaggacaggctagacagcctgccggtgtgttgacggtatcgggtaggcctttaggatcagataaaggggtacctagaacttgacgtctctcttaccgtctcaccgggcgggacacgtttcctacaaggatcggatccacctcgagcgcttgagcgtctgacacggagagaacccgttcctgtccgatctgtcggacggccacacaEcoNImThy15,2605,2805,3005,3205,3405,3605,3805,4005,420
    tgagctagggcactgtggggaaggcagagaacctgtgcagggcagcaatgaacacaggaccagaaaactgcagccctaggaacactcaagagctggccatttgcaagcatctctggcctccgtgcttctcactcatgtcccatgtcttatacaggcctctgtggcacctcgcttgactcgatcccgtgacaccccttccgtctcttggacacgtcccgtcgttacttgtgtcctggtcttttgacgtcgggatccttgtgagttctcgaccggtaaacgttcgtagagaccggaggcacgaagagtgagtacagggtacagaatatgtccggagacaccgtggagcgaacStuImThy15,4405,4605,4805,5005,5205,5405,5605,5805,600
    cctgatctcatccctagccgttaagctttctgcatgacttatcacttggggcataatgctggatacctaccattttcttagaccccatcaaaatcctatttgagtgtacggttcggagaacctcatttatccggtaaatgtcttttactctgctctcagggagctgaggcaggacggactagagtagggatcggcaattcgaaagacgtactgaatagtgaaccccgtattacgacctatggatggtaaaagaatctggggtagttttaggataaactcacatgccaagcctcttggagtaaataggccatttacagaaaatgagacgagagtccctcgactccgtcctgmThy15,6205,6405,6605,6805,7005,7205,7405,760
    atcctgagatacattgggagaggagatacagtttcaataaaataataggttgggtggaggtacatgcctataatgccaccactcaggaaatggtggcagcttcgtgagtttgaggccaacccaagaaacatagtgaaaccctgtcagtaaataagtaagcaagtatttgagtatctaggactctatgtaaccctctcctctatgtcaaagttattttattatccaacccacctccatgtacggatattacggtggtgagtcctttaccaccgtcgaagcactcaaactccggttgggttctttgtatcactttgggacagtcatttattcattcgttcataaactcatagXcmImThy15,7805,8005,8205,8405,8605,8805,9005,9205,940
    tactatatgctagggctgacctggacattaggggtcatcttctgaacaaactagtgcttgagggaggtatttggggtttttgtttgtttaatggatctgaatgagttccagagactggctacacagcgatatgactgagcttaacacccctaaagcatacagtcagaccaattagatgatatacgatcccgactggacctgtaatccccagtagaagacttgtttgatcacgaactccctccataaaccccaaaaacaaacaaattacctagacttactcaaggtctctgaccgatgtgtcgctatactgactcgaattgtggggatttcgtatgtcagtctggttaatcSpeIPflMImThy15,9605,9806,0006,0206,0406,0606,0806,1006,120
    acaataaaaggtatgtatagcttaccaaataaaaaaattgtattttcaagagagtgtctgtctgtgtagccctggctgttcttgaactcactctgtagaccaggctggcctggaaatccatctgcctgcctctgcctctctgcctctctgcctctctgcctctctctctgcctcttgttattttccatacatatcgaatggtttatttttttaacataaaagttctctcacagacagacacatcgggaccgacaagaacttgagtgagacatctggtccgaccggacctttaggtagacggacggagacggagagacggagagacggagagacggagagagagacggagamThy16,1406,1606,1806,2006,2206,2406,2606,2806,300
    ctctgcctctctctgcccctctctgcccctctctgcccctctctgcccctctctgccgccctctgccttctgccctctgccctctggcctctggcctctgccctctgccctctggcctctggcctctgcctctgcctcttgagtgctggaatcaaaggtgtgagctctgtaggtcgagacggagagagacggggagagacggggagagacggggagagacggggagagacggcgggagacggaagacgggagacgggagaccggagaccggagacgggagacgggagaccggagaccggagacggagacggagaactcacgaccttagtttccacactcgagacatccagmThy16,3206,3406,3606,3806,4006,4206,4406,460
    ttaagttccagaagaaagtaatgaagtcacccagcagggaggtgctcagggacagcacagacacacacccaggacataggctcccacttccttggctttctctgagtggcaaaggaccttaggcagtgtcactccctaagagaaggggataaagagaggggctgaggtattcatcaattcaaggtcttctttcattacttcagtgggtcgtccctccacgagtccctgtcgtgtctgtgtgtgggtcctgtatccgagggtgaaggaaccgaaagagactcaccgtttcctggaatccgtcacagtgagggattctcttcccctatttctctccccgactccataagtagmThy16,4806,5006,5206,5406,5606,5806,6006,6206,640
    atgtgctccgtggatctcaagccctcaaggtaaatggggacccacctgtcctaccagctggctgacctgtagctttccccaccacagaatccaagtcggaaccggtaggccaccatggactatggcggcgctttgtctgccgtcggacgcgaacttttgttcgttactaatcctgtacacgaggcacctagagttcgggagttccatttacccctgggtggacaggatggtcgaccgactggacatcgaaaggggtggtgtcttaggttcagccttggccatccggtggtacctgataccgccgcgaaacagacggcagcctgcgcttgaaaacaagcaatgattaggacAgeImThy1hChR2 (H134R)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tggtggtgaacgggtccgtcctggtccctgaggatcaatgttactgtgccggatggattgaatctcgcggcacgaacggcgctcagaccgcgtcaaatgtcctgcagtggcttgcagcaggattcagcattttgctgctgatgttctatgcctaccaaacctggaaatctacatgaccaccacttgcccaggcaggaccagggactcctagttacaatgacacggcctacctaacttagagcgccgtgcttgccgcgagtctggcgcagtttacaggacgtcaccgaacgtcgtcctaagtcgtaaaacgacgactacaagatacggatggtttggacctttagatgtacPflFITth111IhChR2 (H134R)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cggctgggaggagatctatgtgtgcgccattgaaatggttaaggtgattctcgagttcttttttgagtttaagaatccctctatgctctaccttgccacaggacaccgggtgcagtggctgcgctatgcagagtggctgctcacttgtcctgtcatccttatccgcctgagcaacgccgaccctcctctagatacacacgcggtaactttaccaattccactaagagctcaagaaaaaactcaaattcttagggagatacgagatggaacggtgtcctgtggcccacgtcaccgacgcgatacgtctcaccgacgagtgaacaggacagtaggaataggcggactcgttgTliIXhoIPaeR7IhChR2 (H134R)7,0207,0407,0607,0807,1007,1207,1407,160
    ctcaccggcctgagcaacgactacagcaggagaaccatgggactccttgtctcagacatcgggactatcgtgtggggggctaccagcgccatggcaaccggctatgttaaagtcatcttcttttgtcttggattgtgctatggcgcgaacacattttttcacgccgccaaagcatgagtggccggactcgttgctgatgtcgtcctcttggtaccctgaggaacagagtctgtagccctgatagcacaccccccgatggtcgcggtaccgttggccgatacaatttcagtagaagaaaacagaacctaacacgataccgcgcttgtgtaaaaaagtgcggcggtttcgtaAhdIhChR2 (H134R)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    atatcgagggttatcatactgtgccaaagggtcggtgccgccaggtcgtgaccggcatggcatggctgtttttcgtgagctggggtatgttcccaattctcttcattttggggcccgaaggttttggcgtcctgagcgtctatggctccaccgtaggtcacacgattattgatcttatagctcccaatagtatgacacggtttcccagccacggcggtccagcactggccgtaccgtaccgacaaaaagcactcgaccccatacaagggttaagagaagtaaaaccccgggcttccaaaaccgcaggactcgcagataccgaggtggcatccagtgtgctaataactagaApaIPspOMIhChR2 (H134R)7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gatgagtaaaaattgttgggggttgttgggacactacctgcgcgtcctgatccacgagcacatattgattcacggagatatccgcaaaaccaccaaactgaacatcggcggaacggagatcgaggtcgagactctcgtcgaagacgaagccgaggccggagccgtgccagcggccctactcatttttaacaacccccaacaaccctgtgatggacgcgcaggactaggtgctcgtgtataactaagtgcctctataggcgttttggtggtttgacttgtagccgccttgcctctagctccagctctgagagcagcttctgcttcggctccggcctcggcacggtcgccggNotIBfuAIBspMIPshAIhChR2 (H134R)7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gccgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccggcactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgEYFP (no start)7,7207,7407,7607,7807,8007,8207,8407,860
    ccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggggtgggagcactggtggaagccgatgccggacgtcacgaagcgggcgatggggctggtgtacttcgtcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccEYFP (no start)7,8807,9007,9207,9407,9607,9808,0008,0208,040
    cgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgtgttcgacctcatgttgatgttgtcggtgttgcagatatagtaccggctgttcgtcttcttgccgtagttccacttgaagttctaggcggtgttgtagctcctgccgEYFP (no start)8,0608,0808,1008,1208,1408,1608,1808,2008,220
    agcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatcgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctgttggtgatggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtEYFP (no start)8,2408,2608,2808,3008,3208,3408,3608,3808,400
    tggacgagctgtacaagtaagaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcatacctgctcgacatgttcattcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaClaIBspDIBsrGIEcoRIWPREEYFP (no start)8,4208,4408,4608,4808,5008,5208,5408,560
    tttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggPflMIWPRE8,5808,6008,6208,6408,6608,6808,7008,7208,740
    acggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcatgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagtWPRE8,7608,7808,8008,8208,8408,8608,8808,9008,920
    atccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacctcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctactaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggcagctggagctctggatctttttgtacctcgttagtgttcatcgttatgtcgtcgatgClaITliIXhoIBspDISacIIPaeR7IWPRE8,9408,9608,9809,0009,0209,0409,0609,0809,100
    caatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatatcgttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttcccccctgaccttcccgattaagtgagggttgcttctgttctatagKpnIAcc65I9,1209,1409,1609,1809,2009,2209,2409,260
    cttgatctgtggatctaccacacacaaggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgttacaccctggaactagacacctagatggtgtgtgttccgatgaagggactaaccgtcttgatgtgtggtcccggtccctagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcgttctcttccatcttcttcggttacttcctctcttgtgggcgaacaatgtgggac9,2809,3009,3209,3409,3609,3809,4009,4209,440
    tgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaaactcggacgtaccctacctactgggcctctctcttcataatctcacctccaaactgtcggcggatcgtaaagtagtgtaccgggctctcgacgtaggcctgacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaatt3' LTR9,4609,4809,5009,5209,5409,5609,5809,6009,620
    gcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcccgggcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaApaIBclIPmeIPspOMI3' LTR9,6409,6609,6809,7009,7209,7409,7609,7809,800
    ttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctgggaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgaccc9,8209,8409,8609,8809,9009,9209,9409,960
    gatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaagga9,98010,00010,02010,04010,06010,08010,10010,12010,140
    ttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtg10,16010,18010,20010,22010,24010,26010,28010,30010,320
    gttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaattaagacaccttacacacagtcaatcccacacAloIPsiI10,34010,36010,38010,40010,42010,44010,46010,48010,500
    gaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgNsiINsiISexAI10,52010,54010,56010,58010,60010,62010,64010,660
    tccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgataggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactaBclIStuI10,68010,70010,72010,74010,76010,78010,80010,82010,840
    cagcacgtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaaccatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtgtcgtgcacaactgttaattagtagccgtatcatatagccgtatcatattatgctgttccactccttgatttggtaccggttcaactggtcacggcaaggccacgagtggcgcgcgctgcagcggcctcgccagctcaagacctggctggccgagcccaagagggccctgaagcaPmlIMauBIBssHII10,86010,88010,90010,92010,94010,96010,98011,00011,020
    ggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccgcctcctgctgaagcggccacaccaggccctgctgcactgggacaagtagtcgcgccaggtcctggtccaccacggcctgttgtgggaccggacccacacccacgcgccggacctgctcgacatgcggctcaccagcctccagcacaggtgcttgaaggccctgcggaggcccggcBmgBISexAISgrAI11,04011,06011,08011,10011,12011,14011,16011,18011,200
    gccatgaccgagatcggcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggactgacacgtgctacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccgcggtactggctctagccgctcgtcggcacccccgccctcaagcgggacgcgctgggccggccgttgacgcacgtgaagcaccggctcctcgtcctgactgtgcacgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggcFseIPmlI11,22011,24011,26011,28011,30011,32011,34011,360
    gctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttacgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatPsiI11,38011,40011,42011,44011,46011,48011,50011,52011,540
    tcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgcagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgBstZ17I11,56011,58011,60011,62011,64011,66011,68011,70011,720
    tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaaagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattSapIBspQI11,74011,76011,78011,80011,82011,84011,86011,88011,900
    tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtccPciI11,92011,94011,96011,98012,00012,02012,04012,060
    actataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccg12,08012,10012,12012,14012,16012,18012,20012,22012,240
    tgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattga12,26012,28012,30012,32012,34012,36012,38012,40012,420
    acggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttttgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaa12,44012,46012,48012,50012,52012,54012,56012,58012,600
    gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcctagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagBspHI12,62012,64012,66012,68012,70012,72012,74012,760
    agtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccgtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggcAhdI12,78012,80012,82012,84012,86012,88012,90012,92012,940
    gaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgagAclIFspI12,96012,98013,00013,02013,04013,06013,08013,10013,120
    cggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagaPvuI13,14013,16013,18013,20013,22013,24013,26013,28013,300
    gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactAclIScaI13,32013,34013,36013,38013,40013,42013,44013,460
    gatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaSspI13,48013,50013,52013,54013,56013,58013,60013,62013,640
    ttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgBspHI13,66013,68013,70013,72013,740
    • Plasmid
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    13753 bp10000110001200013000100020003000mThy1

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    13753