Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
DE_3xStop
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    catgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGDrdIPciIBssSIAflIIIBssSαIpMB1-pUC19 ORI variant20406080100120140160
    GTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAApaLIBaeGIBciVIBseYIBsiEIpMB1-pUC19 ORI variant180200220240260280300320340
    TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGaACAGTATTTGGTATCTGCGCTCTATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCtTGTCATAAACCATAGACGCGAGAAlwNIpMB1-pUC19 ORI variant360380400420440460480500520
    GCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAgaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaacCGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgAcuIpMB1-pUC19 ORI variant540560580600620640660680700
    tcacgttaagggattttggtcatgcattctaggtgattattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatacagtgcaattccctaaaaccagtacgtaagatccactaataataaacggctgatggaaccactagagcggaaagtgcatcacctgtttaagaaggttgactagacgcgcgctccggttcgctagaagaagaacaggttctattcggacagatcgaagttcatactgcccgactatgApoIBsmINsiIBsaAIBssHIIDraIIISpec geneSpecRpro-CDS-Ter of SpecR720740760780800820840860
    tgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatacccggccgtccgcgaggtaacgggtcagccgtcgctgtaggaagccgcgctaaaacggccaatgacgcgacatggtttacgccctgttgcattcgtgatgtaaagcgagtagcggtcgggtcagcccgccgctcaaggtatcgcaattccaaagtaaatcgcggagtttatctaBglIFauINaeIBsmFIBsrDIBsrFIBsrFIBtgZINgoMIVSau96ISpec geneSpecRpro-CDS-Ter of SpecR8809009209409609801,0001,0201,040
    cctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagataccagcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagcggacaagtccttggcctagtttctcaaggaggcggcgacctggatggttccgttgcgatacaagagaacgaaaacagtcgttctatcggtctagttacagctagcaccgaccgagcttctatggtcgttcttacagtaacgcgacggtaagaggtttaacgtcaagcgcgaatcgBlpIPvuIAvaIIBsiEIBsrDISau96ISpec geneSpecRpro-CDS-Ter of SpecRSpec geneSpecRpro-CDS-Ter of SpecRSpecR RSpecR F1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcactctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtacctattgcggtgccttactacagcagcacgtgttgttaccactgaagatgtcgcgcctcttagagtgagagaggtccccttcggcttcaaaggttttccagcaactagtttcgagcggcgcaacaaagtagttcggaatgccagtggcattggtcgtttagttatagtgacacaAcuIAjuIBpmIBtgIMslITfiIApaLIBaeGIBstEIIDraIIITsp45ITsp45ISpec geneSpecRpro-CDS-Ter of SpecR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatgcgaaacgatcctcatcctgtctcttgatcagatattgatccccccgaagtccggcggtaggtgacgcctcggcatgtttacatgccggtcgttgcagccaagctctaccgcgagctactgcggttgatggagactatcaactcagctatgaagccgctagtggcgaagggagtacgctttgctaggagtaggacagagaactagtctataactaggggBtsIEaeIBsaBIBsaBIBtsαIpro-CDS-Ter of SpecRSpec geneSpecRspecR_promoter_from_pKD131,4201,4401,4601,4801,5001,5201,5401,560
    tgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttaccagagggcgccccagctggcaattccggttcgcttgctaagcttgcatgcctgcaagtcgactctagaggagcggtgatcacaggcagcaacgctctgtcatcgttacaacgcggtagtctaggaaccgccgttctttcggtaggtcaaatgaaacgtcccgaagggttggaatggtctcccgcggggtcgaccgttaaggccaagcgaacgattcgaacgtacggacgttcagctgagatctcctcgccactagtgtccgtcgttgcgagacagtagcaatgtAccIBanIBlpIKasINarISalISfoISphIXbaIXcmIBseRIBseYIBsrBIPluTIPvuIIHincIIHindIIIpro-CDS-Ter of SpecRspecR_promoter_from_pKD131,5801,6001,6201,6401,6601,6801,7001,7201,740
    atcaacatgctaccctccgcgagatcatccgtgtttcaaacccggcagcttagttgccgttcttccgaatagcatcggtaacatgagcaaagtctgccgccttacaacggctctcccgctgacgccgtcccggactgatgggctgcctgtatcgagtggtgattttgtgccgagctagttgtacgatgggaggcgctctagtaggcacaaagtttgggccgtcgaatcaacggcaagaaggcttatcgtagccattgtactcgtttcagacggcggaatgttgccgagagggcgactgcggcagggcctgactacccgacggacatagctcaccactaaaacacggctcgFauIPfoIBsmFIPflFIBstAPIEco53kITth111IIn T-DNA region AH003392.21,7601,7801,8001,8201,8401,8601,8801,9001,920
    tcattactcgcatccattctcaggctgtctcgtctcgtctcAGGTCTCCAGGTTAATGATAGGCTTCGAGACCAccaggatacatagattaccacaactccgagcccttccacccacagaatcaggggataacgcaggaaagaaagtaatgagcgtaggtaagagtccgacagagcagagcagagTCCAGAGGTCCAATTACTATCCGAAGCTCTGGTggtcctatgtatctaatggtgttgaggctcgggaaggtgggtgtcttagtcccctattgcgtcctttcttBpmIBsaIBsaISacITfiIBanIIBanIIBciVIBsaXIBsmBIBsmBIEsp3IEsp3INmeAIII*1*2*3UNS1UNSXExtra region of pBR3221,9401,9601,9802,0002,0202,0402,060
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    2069 bp15001750250500pMB1-pUC19 ORI variantpro-CDS-Ter of SpecRMut C->ABspMIN spacer1979-1987PUC_VF (DNA)SpR (DNA)UXR (DNA)

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    2069

    Welcome to Benchling!

    Isaac Núñez has shared a file with you.