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YH038 PGK-TO-FRB-tm-MCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    tctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctccagatggcccatcccctccgcgaaaagggttccgtcagacctcgtacgcgaaatcgtcggggcgacccgtgaaccgcgatgtgttcaccggagaccggagcgtgtgtaaggtgtaggtggccatccgcggttggccgaggcaagaaaccaccggggaagcgcggtggaagatgaggaggPGK20406080100120140160
    cctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcattagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggggatcagtccttcaagggggggcggggcgtcgagcgcagcacgtcctgcactgtttaccttcatcgtgcagagtaatcagagcacgtctacctgtcgtggcgactcgttaccttcgcccatccggaaaccccgtcgccggttatcgtcgaaacgaggaagcgaaagacccgagtctccBlpIPGK180200220240260280300320340
    ctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctgcaggactctagcgtttaaacttagacccttccccacccaggcccccgcccgagtccccgcccgagtccccgccccgcccgcgggcttccaggaggcctccgggccgtaagacgtgcgaagttttcgcgtgcagacggcgcgacaagaggagaaggagtagaggcccggaaagctggacgtcctgagatcgcaaatttgaatBsmIPmeIPstISbfIAflIIBfuAIBspMIPGK360380400420440460480500520
    agcttcgccaccatggcgctccctgtcaccgcactgcttcttccgctggcactgctgctgcacgctgcacggcctggatccctggagatgtggcatgaaggcctggaagaggcatctcgtttgtactttggggaaaggaacgtgaaaggcatgtttgaggtgctggagcccttgcatgtcgaagcggtggtaccgcgagggacagtggcgtgacgaagaaggcgaccgtgacgacgacgtgcgacgtgccggacctagggacctctacaccgtacttccggaccttctccgtagagcaaacatgaaacccctttccttgcactttccgtacaaactccacgacctcgggaacgtacBamHIEcoNIHindIIIG1S2L1E2M3W4H5E6G7L8E9E10A11S12R13L14Y15F16G17E18R19N20V21K22G23M24F25E26V27L28E29P30L31H32616-891M1A2L3P4V5T6A7L8L9L10P11L12A13L14L15L16H17A18A19R20P21547-609FRBCD8α signal peptide540560580600620640660680700
    ctatgatggaacggGGACCCcagactctgaaggaaacatcctttaatcaggcctatggtcgagatttaatggaggcccaagagtggtgcaggaagtacatgaaatcagggaatgtcaaggacctcctccaagcctgggacctctattatcatgtgttccgacgaatctcaaagactaggatactaccttgccCCTGGGgtctgagacttcctttgtaggaaattagtccggataccagctctaaattacctccgggttctcaccacgtccttcatgtactttagtcccttacagttcctggaggaggttcggaccctggagataatagtacacaaggctgcttagagtttctgatcKflIA33M34M35E36R37G38P39Q40T41L42K43E44T45S46F47N48Q49A50Y51G52R53D54L55M56E57A58Q59E60W61C62R63K64Y65M66K67S68G69N70V71K72D73L74L75Q76A77W78D79L80Y81Y82H83V84F85R86R87I88S89K90T91R92616-891FRB720740760780800820840860880
    aggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacagtggcttccgcggccctgtcctcccagacccccaccgtgaccgcctagacctcctctgatgttcctgctactgctgttgccgcctagcccccccagaccaccatcaccgccaagcccccctccatggccagaccagcaccaacgacctcaggacaaaacaataccgaacgatcactgtcaccgaKpnIPsrIAcc65IL1V2V3V4A5G6V7L8F9C10Y11G12L13L14V15T16V17A181015-1077G1A2G3T4G5G6S7G8G9G10T11G12G13S14G15G16D17Y18K19D20D21D22D23N24G25G26S27G28G29S30G31G32S33G34G35S36G37G38G39T40G41892-101445-ECLCD28-TMD10aa linker9009209409609801,0001,0201,0401,060
    ctttgtgttgaaaacggcGGTGGTAGCGCTATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCgaaacacaacttttgccgCCACCATCGCGATACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGAfeIMA2SN4FT6QF8VL10VD12NG14GT16GD18VT20VA22PS24NF26AN28GI30AE32WI34SS36NS38RS40QA42YK44VT46CS48V1099-1824S1A2G1G2L19C20V21E1N2G3MCP1,0801,1001,1201,1401,1601,1801,2001,2201,240
    GCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGXcmIBsaBIBstEIIR50QS52SA54QN56RK58YT60IK62VE64VP66KG68AW70RS72YL74NM76EL78TI80PI82FA84TN86SD88CE90LI92VK94AM96QG98LL100KD102GN104PI106PS108A1099-1824MCP1,2601,2801,3001,3201,3401,3601,3801,4001,420
    GATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATABplIBplIAsiSII110AA112NS114GI116YG118GG120TG122GG124SM126AS128NF130TQ132FV134LV136DN138GG140TG142DV144TV146AP148SN150FA152NG154IA156EW158IS160SN162SR164SQ166AY1681099-1824MCPMCP1,4401,4601,4801,5001,5201,5401,5601,5801,600
    AAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGTTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACBstXIBstZ17IKV170TC172SV174RQ176SS178AQ180NR182KY184TI186KV188EV190PK192GA194WR196SY198LN200ME202LT204IP206IF208AT210NS212DC214EL216IV218KA220MQ222GL224LK226D1099-1824MCP1,6201,6401,6601,6801,7001,7201,7401,7601,780
    GCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGAATTTACtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGCCTTAAATGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggApaIAvaIBclIPmeITliIXhoIBsoBIPspXIPaeR7IPspOMIH1H2H3H4H5H61891-1908G228NP230IP232SA234IA236AN238SG240IY2421099-1824*1MCPc-myc epitopeBGH polyade...n sequencePolyhi...e tag1,8001,8201,8401,8601,8801,9001,9201,940
    atctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcattagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtaBbsIBtgZIBGH polyadenylation sequence1,9601,9802,0002,0202,0402,0602,0802,1002,120
    gctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgcgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacClaIBglIIBspDIPvuIIBGH pA2,1402,1602,1802,2002,2202,2402,2602,2802,300
    ccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctaggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggatKflINaeIBsaAINgoMIVFRT2,3202,3402,3602,3802,4002,4202,4402,4602,480
    ttccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctaBarIMscIXbaIPflFIPshAITth111IHygromycinFRT2,5002,5202,5402,5602,5802,6002,6202,6402,660
    atgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactEcoRIDraIIIHygromycin2,6802,7002,7202,7402,7602,7802,8002,8202,840
    aaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgaNdeIPstIAsiSIBfuAIBspMIRsrIIHygromycin2,8602,8802,9002,9202,9402,9602,9803,0003,020
    gtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggcactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagccccPflFIDraIIITth111IHygromycin3,0403,0603,0803,1003,1203,1403,1603,1803,200
    attcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaattttaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaaAjuIBtgZISacIIHygromycin3,2203,2403,2603,2803,3003,3203,3403,3603,380
    cgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataggctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcScaIHygromycin3,4003,4203,4403,4603,4803,5003,5203,5403,560
    cacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcagtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtNaeIPfoIPsiIBsaAINgoMIVSV40 pA3,5803,6003,6203,6403,6603,6803,7003,720
    caaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacggtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgcBsmIBstZ17ISV40 pA3,7403,7603,7803,8003,8203,8403,8603,8803,900
    agccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgtcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcSapIBspQIPvuII3,9203,9403,9603,9804,0004,0204,0404,0604,080
    ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgPciIAflIIIpUC ori4,1004,1204,1404,1604,1804,2004,2204,2404,260
    tccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgapUC ori4,2804,3004,3204,3404,3604,3804,4004,4204,440
    ttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtccpUC ori4,4604,4804,5004,5204,5404,5604,5804,6004,620
    attagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaapUC ori4,6404,6604,6804,7004,7204,7404,7604,7804,800
    ttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagBspHIpUC ori4,8204,8404,8604,8804,9004,9204,9404,9604,980
    aatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgattagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctAhdIBmrIBsaIAmp (bla)5,0005,0205,0405,0605,0805,1005,1205,1405,160
    gacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacagctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtcAclIFspIAmp (bla)5,1805,2005,2205,2405,2605,2805,3005,3205,340
    gcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactcgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgaAmp (bla)5,3605,3805,4005,4205,4405,4605,4805,500
    gcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgtcgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaAclIBcgIScaIXmnIAmp (bla)5,5205,5405,5605,5805,6005,6205,6405,6605,680
    tcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttAmp (bla)5,7005,7205,7405,7605,7805,8005,8205,8405,860
    tactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgSspIBglIIBspHISgrDIbla promoter5,8805,9005,9205,9405,9605,9806,0006,0206,040
    actctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacggtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgccMfeIBsiWI6,0606,0806,1006,1206,1406,1606,1806,2006,220
    gccagatatacgcgtcggtctatatgcgcaMluIAflIII6,240
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6245 bp45005000550050010001500MCP10aa linker1099-1824nb139_F (DNA)oLSM0264 (DNA)EKo272.2 (DNA)EKbF (DNA)oLSM0275 (DNA)CCp027-AV373Kozak-f (DNA)YH029.1 (DNA)CCp080 Cpep-F (DNA)p450_F (DNA)p450_F (DNA)pPYK021 (DNA)pPYK008 (DNA)EKo283.2 (DNA)pNSK174 (DNA)XZp112.2 (DNA)EKo492.1 (DNA)EKo331.2 (DNA)EKo472.1 (DNA)EKo454.1 (DNA)EKo329.5 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)oLSM039 (DNA)FlpInF2 (DNA)EKo152.4 (DNA)pNSK309 (DNA)YH038.1 (DNA)EKo279.1 (DNA)PGKBlpIBsmI,PstI,SbfIKozak,CD8α signal peptide,547-609,YH038.2 (DNA),+8EKo93.1 (DNA)610-615,EKo39.1 (DNA),EKo331.3 (DNA),+3KflIFRB,616-891Rapalog MutEKo329.2 (DNA)EK-ECLF (DNA),EKo35.2 (DNA)45-ECL,892-1014,EK-ECLR (DNA)EKo331.1 (DNA),Acc65I,KpnI,PsrICD28-TMD,1015-1077,XZp017.2 (DNA)1078-10861087-1092AfeI1093-1098MCP,XcmIBsaBIBstEIIAsiSIBplIBspHIAhdIBmrIBsaIFspIAclIAmp (bla)ScaIBcgIAclIXmnISspIbla promoter,BspHI,SgrDI,BglIIFlpInF1 (DNA),FlpInF (DNA),EF1a_F (DNA),EF1a_F (DNA),+6

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    6245