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YH038 PGK-TO-FRB-tm-MCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    tctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactccagatggcccatcccctccgcgaaaagggttccgtcagacctcgtacgcgaaatcgtcggggcgacccgtgaaccgcgatgtgttcaccggagaccggagcgtgtgtaaggtgtaggtggccatccgcggttggccgaggcaagaaaccaccggggaagcgcggtggaagatgaggPGK20406080100120140160
    tcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcattagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctaggggatcagtccttcaagggggggcggggcgtcgagcgcagcacgtcctgcactgtttaccttcatcgtgcagagtaatcagagcacgtctacctgtcgtggcgactcgttaccttcgcccatccggaaaccccgtcgccggttatcgtcgaaacgaggaagcgaaagacccgaBlpIPGK180200220240260280300320340
    cagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctgcaggactctagcgtgtctccgacccttccccacccaggcccccgcccgagtccccgcccgagtccccgccccgcccgcgggcttccaggaggcctccgggccgtaagacgtgcgaagttttcgcgtgcagacggcgcgacaagaggagaaggagtagaggcccggaaagctggacgtcctgagatcgcaBsmIPstISbfIBfuAIBspMIPGK360380400420440460480500520
    ttaaacttaagcttcgccaccatggcgctccctgtcaccgcactgcttcttccgctggcactgctgctgcacgctgcacggcctggatccctggagatgtggcatgaaggcctggaagaggcatctcgtttgtactttggggaaaggaacgtgaaaggcatgtttgaggtgctggaatttgaattcgaagcggtggtaccgcgagggacagtggcgtgacgaagaaggcgaccgtgacgacgacgtgcgacgtgccggacctagggacctctacaccgtacttccggaccttctccgtagagcaaacatgaaacccctttccttgcactttccgtacaaactccacgaccPmeIAflIIBamHIEcoNIHindIIIM1A2L3P4V5T6A7L8L9L10P11L12A13L14L15L16H17A18A19R20P21547-609L1E2M3W4H5E6G7L8E9E10A11S12R13L14Y15F16G17E18R19N20V21K22G23M24F25E26V27L28616-891G1S2FRBCD8α signal peptide540560580600620640660680700
    agcccttgcatgctatgatggaacggGGACCCcagactctgaaggaaacatcctttaatcaggcctatggtcgagatttaatggaggcccaagagtggtgcaggaagtacatgaaatcagggaatgtcaaggacctcctccaagcctgggacctctattatcatgtgttccgacgtcgggaacgtacgatactaccttgccCCTGGGgtctgagacttcctttgtaggaaattagtccggataccagctctaaattacctccgggttctcaccacgtccttcatgtactttagtcccttacagttcctggaggaggttcggaccctggagataatagtacacaaggctgcKflIE29P30L31H32A33M34M35E36R37G38P39Q40T41L42K43E44T45S46F47N48Q49A50Y51G52R53D54L55M56E57A58Q59E60W61C62R63K64Y65M66K67S68G69N70V71K72D73L74L75Q76A77W78D79L80Y81Y82H83V84F85R86R87616-891FRB720740760780800820840860
    aatctcaaagactagaggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtctggtcgtggttgctggagtcctgttttgttatggcttagagtttctgatctccgcggccctgtcctcccagacccccaccgtgaccgcctagacctcctctgatgttcctgctactgctgttgccgcctagcccccccagaccaccatcaccgccaagcccccctccatggccagaccagcaccaacgacctcaggacaaaacaataccgKpnIPsrIAcc65IG1A2G3T4G5G6S7G8G9G10T11G12G13S14G15G16D17Y18K19D20D21D22D23N24G25G26S27G28G29S30G31G32S33G34G35S36G37G38G39T40G41892-1014L1V2V3V4A5G6V7L8F9C10Y11G121015-1077I88S89K90T91R92616-89145-ECLCD28-TMD10aa linkerFRB8809009209409609801,0001,0201,040
    ttgctagtgacagtggctctttgtgttgaaaacggcGGTGGTAGCGCTATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATaacgatcactgtcaccgagaaacacaacttttgccgCCACCATCGCGATACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTAAfeIS1A2E1N2G3G1G2L13L14V15T16V17A18L19C20V211015-1077MA2SN4FT6QF8VL10VD12NG14GT16GD18VT20VA22PS24NF26AN28GI30AE32WI34SS36NS38RS40QA421099-1824MCPCD28-TMD1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTXcmIBsaBIYK44VT46CS48VR50QS52SA54QN56RK58YT60IK62VE64VP66KG68AW70RS72YL74NM76EL78TI80PI82FA84TN86SD88CE90LI92VK94AM96QG98LL100K1099-1824MCP1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGBplIAsiSIBstEIID102GN104PI106PS108AI110AA112NS114GI116YG118GG120TG122GG124SM126AS128NF130TQ132FV134LV136DN138GG140TG142DV144TV146AP148SN150FA152NG154IA156EW158I1099-1824MCPMCP1,4201,4401,4601,4801,5001,5201,5401,560
    TCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACBplIBstXIBstZ17IS160SN162SR164SQ166AY168KV170TC172SV174RQ176SS178AQ180NR182KY184TI186KV188EV190PK192GA194WR196SY198LN200ME202LT204IP206IF208AT210NS212DC214EL216I1099-1824MCP1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGAATTTACtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGCCTTAAATGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgacApaIAvaIBclIPmeITliIXhoIBsoBIPspXIPaeR7IPspOMIV218KA220MQ222GL224LK226DG228NP230IP232SA234IA236AN238SG240IY2421099-1824H1H2H3H4H5H61891-1908*1MCPc-myc epitopePolyhi...e tag1,7601,7801,8001,8201,8401,8601,8801,9001,920
    atcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgBtgZIBGH polyadenylation sequence1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccBbsIClaIBglIIBspDIPvuIIBGH polyadenylation sequenceBGH pA2,1202,1402,1602,1802,2002,2202,2402,260
    cgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaagcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggtttKflINaeINgoMIV2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggaggtttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctccBarIMscIXbaIBsaAIPflFIPshAITth111IHygromycinFRT2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctacgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggatEcoRIHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ttgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggaacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccPstIAsiSIBfuAIBspMIRsrIIDraIIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    cgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgagcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactNdeIPflFIDraIIITth111IHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgAjuIBtgZISacIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtacatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagtcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaNaeIPfoIScaIBsaAINgoMIVHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    tcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgBsmIPsiIBstZ17ISV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtc3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagagacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttctSapIBspQIPvuII4,0404,0604,0804,1004,1204,1404,1604,1804,200
    acatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctcccttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggaPciIAflIIIpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    cgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccgcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctcaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaaacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttpUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ctcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcagagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtBspHIAmp (bla)4,9204,9404,9604,9805,0005,0205,0405,060
    tccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcAhdIBmrIBsaIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtaAclIFspIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagcaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcBcgIScaIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactaAclIXmnIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    cttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaactSspIBspHIAmp (bla)bla promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
    atgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgtacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcBglIISgrDIbla promoter5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgtgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaMfeIMluIBsiWIAflIII6,1406,1606,1806,2006,2206,240
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6245 bp45005000550050010001500MCP10aa linker1099-1824nb139_F (DNA)oLSM0264 (DNA)EKbF (DNA)EKo272.2 (DNA)oLSM0275 (DNA)CCp027-AV373Kozak-f (DNA)CCp080 Cpep-F (DNA)YH029.1 (DNA)p450_F (DNA)p450_F (DNA)pPYK008 (DNA)pPYK021 (DNA)EKo283.2 (DNA)pNSK174 (DNA)XZp112.2 (DNA)EKo492.1 (DNA)EKo331.2 (DNA)EKo472.1 (DNA)EKo454.1 (DNA)EKo329.5 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)oLSM039 (DNA)FlpInF2 (DNA)EKo152.4 (DNA)YH038.1 (DNA)pNSK309 (DNA)EKo279.1 (DNA)PGKBlpIBsmI,PstI,SbfIKozak,CD8α signal peptide,547-609,YH038.2 (DNA),+8EKo93.1 (DNA)610-615,EKo39.1 (DNA),EKo331.3 (DNA),+3KflIFRB,616-891Rapalog MutEKo329.2 (DNA)EK-ECLF (DNA),EKo35.2 (DNA)45-ECL,892-1014,EK-ECLR (DNA)EKo331.1 (DNA),Acc65I,KpnI,PsrICD28-TMD,1015-1077,XZp017.2 (DNA)1078-10861087-1092AfeI1093-1098MCP,XcmIBsaBIBstEIIAsiSIBplIBspHIAhdIBmrIBsaIFspIAclIAmp (bla)ScaIBcgIAclIXmnISspIbla promoter,BspHI,SgrDI,BglIIFlpInF1 (DNA),FlpInF (DNA),EF1a_F (DNA),EF1a_F (DNA),+6

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    6245

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