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LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggW286PCR XZ07920406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228174-1031: Region 2HK284IL282SA280GI278EA276I174-1031: Region 1AmpPCR XZ079180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaFspIWG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170174-1031: Region 2AmpPCR XZ079360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattEN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112L174-1031: Region 2AmpPCR XZ079540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaScaIH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54K174-1031: Region 2AmpPCR XZ079720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M174-1031: Region 2AmpPCR XZ0798809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaCspCIPCR XZ0791,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggAloIPsiIPCR XZ0791,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattPCR XZ0791,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagFspIPCR XZ0791,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataa5' PB ITR Longjt1009PCR XZ0791,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataatttttttt5' PB ITR Long5' PB ITR shortPCR XZ0791,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccRsrIIcore insul5' PB ITR LongPCR XZ0792,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccactagtTTCGCACGATTGCACCTTGGGAGCCTTAACCGCTTTTGAATGTCCGTCTTAGTTAATctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgatcaAAGCGTGCTAACGTGGAACCCTCGGAATTGGCGAAAACTTACAGGCAGAATCAATTASfiISpeISrfIcore insulattD Cp36Forward Targettarget ...primerPCR XZ0792,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCCTAATTGAACGCTCCAAAAGGTGACTTCCAATAGGGATTTATTCCTTTTAAAATTAACGGCATAATCGTGCCGCCGAATGCAACCTCGATCGAGCCGGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGGGATTAACTTGCGAGGTTTTCCACTGAAGGTTATCCCTAAATAAGGAAAATTTTAATTGCCGTATTAGCACGGCGGCTTACGTTGGAGCTAGCTCGGCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAMfeIEF-1α promoterattD Cp36Primer 1Reverse TargetTarget ...Primerprime...F-1aPCR XZ0792,4602,4802,5002,5202,5402,5602,5802,6002,620
    GAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGEF-1α promoterPCR XZ0792,6402,6602,6802,7002,7202,7402,7602,7802,800
    CCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGAflIISacIIEF-1α promoterPCR XZ0792,8202,8402,8602,8802,9002,9202,9402,960
    GCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGBglIIEF-1α promoterPCR XZ0792,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTFseISacIIEF-1α promoterPCR XZ0793,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGBbvCIEF-1α promoterPCR XZ0793,3403,3603,3803,4003,4203,4403,4603,4803,500
    ACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAEF-1α promoterfrag1PCR XZ0793,5203,5403,5603,5803,6003,6203,6403,660
    CAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAATCTAGAAGCCACCatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatatgctcatggcctctaccacaagcgcCgGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTTAGATCTTCGGTGGtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctatacgagtaccggagatggtgttcgcgGcClaIXbaIBspDIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28LM30AS32TT34SA363742-6909AVPR2 (Tango)Signal/FlagEF-1α promoterfrag1RF from EF-1ahomologhomologPCR XZ0793,6803,7003,7203,7403,7603,7803,8003,8203,840
    tgcccggacatccatctcttccaagtcttccctccaacagctcacaggagaggcctctggatacccgggacccacttcttgcaagagcggagctcgccttgctctccatcgttttcgttgccgtcgcgttgagcaatggtctggtgctggccgccctggcgcgacgaggccgccgacgggcctgtaggtagagaaggttcagaagggaggttgtcgagtgtcctctccggagacctatgggccctgggtgaagaacgttctcgcctcgagcggaacgagaggtagcaaaagcaacggcagcgcaactcgttaccagaccacgaccggcgggaccgcgctgctccggcggcVP38GH40PS42LP44SL46PS48NS50SQ52ER54PL56DT58RD60PL62LA64RA66EL68AL70LS72IV74FV76AV78AL80SN82GL84VL86AA88LA90RR92GR94R3742-6909AVPR2 (Tango)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aggccactgggcgcctattcatgtctttatcggccatctctgtcttgcagacctggccgtggcgctgtttcaggtgctccctcaactggcttggaaagccaccgatagattccgggggccagacgcactgtgtcgggccgtgaaatacctgcagatggtgggaatgtatgccagttccggtgacccgcggataagtacagaaatagccggtagagacagaacgtctggaccggcaccgcgacaaagtccacgagggagttgaccgaacctttcggtggctatctaaggcccccggtctgcgtgacacagcccggcactttatggacgtctaccacccttacatacggtcaSfiIG96HW98AP100IH102VF104IG106HL108CL110AD112LA114VA116LF118QV120LP122QL124AW126KA128TD130RF132RG134PD136AL138CR140AV142KY144LQ146MV148GM150YA152S3742-6909AVPR2 (Tango)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcatatatgatacttgctatgaccttggacagacacagggctatctgtcgccccatgctggcgtataggcacggttccggagcccattggaacaggccagttctggttgcgtgggctttttccctgcttctgagcttgccccagttgtttattttcgcacagagaaatgtggaggagtatatactatgaacgatactggaacctgtctgtgtcccgatagacagcggggtacgaccgcatatccgtgccaaggcctcgggtaaccttgtccggtcaagaccaacgcacccgaaaaagggacgaagactcgaacggggtcaacaaataaaagcgtgtctctttacacctccS154YM156IL158AM160TL162DR164HR166AI168CR170PM172LA174YR176HG178SG180AH182WN184RP186VL188VA190WA192FS194LL196LS198LP200QL202FI204FA206QR208NV210E3742-6909AVPR2 (Tango)4,2204,2404,2604,2804,3004,3204,3404,360
    gcggatctggcgtgaccgattgctgggcttgcttcgctgagccatggggcagaaggacctacgtgacgtggattgctctcatggtgttcgttgctcccaccctggggatcgccgcatgccaggtcctgatattcagagaaatacacgcctccttggttcctgggccaagcgagcgcgcctagaccgcactggctaacgacccgaacgaagcgactcggtaccccgtcttcctggatgcactgcacctaacgagagtaccacaagcaacgagggtgggacccctagcggcgtacggtccaggactataagtctctttatgtgcggaggaaccaaggacccggttcgctcgcAlwNIG212GS214GV216TD218CW220AC222FA224EP226WG228RR230TY232VT234WI236AL238MV240FV242AP244TL246GI248AA250CQ252VL254IF256RE258IH260AS262LV264PG266PS268ER2703742-6909AVPR2 (Tango)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ccctggtggaagaagacggggtcgaaggactggctctccaggcgaaggcgcccacgtatcagctgcggtagctaagaccgtacgcatgacactggtcatcgtcgtcgtttacgttctttgctgggccccgttttttttggtgcagctctgggcggcctgggaccccgaagcacccgggaccaccttcttctgccccagcttcctgaccgagaggtccgcttccgcgggtgcatagtcgacgccatcgattctggcatgcgtactgtgaccagtagcagcagcaaatgcaagaaacgacccggggcaaaaaaaaccacgtcgagacccgccggaccctggggcttcgtgggPG272GR274RR276GR278RT280GS282PG284EG286AH288VS290AA292VA294KT296VR298MT300LV302IV304VV306YV308LC310WA312PF314FL316VQ318LW320AA322WD324PE326AP3283742-6909AVPR2 (Tango)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ctggaaggtgctccattcgttctgctcatgcttctcgcttcactgaatagctgtaccaatccttggatttacgcctctttctcctccagtgttagctccgagctgcggagtcttctctgttgcgcccggggcaggacccccccaagcctgggtccacaggacgaatcctgcactagaccttccacgaggtaagcaagacgagtacgaagagcgaagtgacttatcgacatggttaggaacctaaatgcggagaaagaggaggtcacaatcgaggctcgacgcctcagaagagacaacgcgggccccgtcctgggggggttcggacccaggtgtcctgcttaggacgtgatPflMILE330GA332PF334VL336LM338LL340AS342LN344SC346TN348PW350IY352AS354FS356SS358VS360SE362LR364SL366LC368CA370RG372RT374PP376SL378GP380QD382ES384CT3863742-6909AVPR2 (Tango)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ccgcctccagttccctggccaaagacacctcttctatcgataccggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATggcggaggtcaagggaccggtttctgtggagaagatagctatggccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAClaIKpnIXcmIBspDIAcc65ITA388SS390SL392AK394DT396SS398ID400TG402GR404TP406PS408LG410PQ412DE414SC416TT418AS420SS422LA424KD426TS428SG430TG432AL434HL436DQ438TP440SR442QP444I3742-6909AVPR2 (Tango)V2 tailhADAR2 cat domainhomologous4,9204,9404,9604,9805,0005,0205,0405,060
    TCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACP446SE448GL450QL452HL454PQ456VL458AD460AV462SR464LV466LG468KF470GD472LT474DN476FS478SP480HA482RR484KV486LA488GV490VM492TT494GT496DV498KD500AK502V3742-6909hADAR2 cat domain5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGI504SV506ST508GT510KC512IN514GE516YM518SD520RG522LA524LN526DC528HA530EI532IS534RR536SL538LR540FL542YT544QL546EL548YL550NN552KD554DQ556KR558SI560F3742-6909hADAR2 cat domain5,2605,2805,3005,3205,3405,3605,3805,4005,420
    AGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGATCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTPfoIBsrGIQ562KS564ER566GG568FR570LK572EN574VQ576FH578LY580IS582TS584PC586GD588AR590IF592SP594HE596PI598LE600EP602AD604RH606PN608RK610AR612GQ614LR616TK618IE6203742-6909hADAR2 cat domain5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCBamHISG622QG624TI626PV628RS630NA632SI634QA636WD638GV640LQ642GE644RL646LT648MS650CS652DK654IA656RW658NV660VG662IQ664GS666LL668SI670FV672EP674IY676FS6783742-6909hADAR2 cat domain5,6205,6405,6605,6805,7005,7205,7405,760
    AGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGAHincIISI680IL682GS684LY686HG688DH690LS692RA694MY696QR698IS700NI702ED704LP706PL708YT710LN712KP714LL716SG718IS720NA722EA724RQ726PG728KA730PN732FS734VN7363742-6909hADAR2 cat domain5,7805,8005,8205,8405,8605,8805,9005,9205,940
    GGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTMluIWT738VG740DS742AI744EV746IN748AT750TG752KD754EL756GR758AS760RL762CK764HA766LY768CR770WM772RV774HG776KV778PS780HL782LR784SK786IT788KP790NV792YH794E3742-6909hADAR2 cat domain5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCGAAGGTCGGGGGAGTTTGCTTACCTGTGGCGATGTCGAGGAGAATCCAGGGCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGCTTCCAGCCCCCTCAAACGAATGGACACCGCTACAGCTCCTCTTAGGTCCCS796KL798AA800KE802YQ804AA806KA808RL810FT812AF814IK816AG818LG820AW822VE824KP826TE828QD830QF832SL834TP836EG838RG840SL842LT844CG846DV848EE850NP852G3742-6909hADAR2 cat domainT2APCR XZ0796,1406,1606,1806,2006,2206,2406,2606,2806,300
    CCCAAGCTTATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGGGTTCGAATACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGRsrIIHindIIIP854KL856MT858EY860KP862TV864RL866AT868RD870DV872PR874AV876RT878LA880AA882FA884DY886PA888TR890HT892VD894PD896RH898IE900RV902TE904LQ906EL908FL910T3742-6909PuroRPR_S030PR_S029PCR XZ0796,3206,3406,3606,3806,4006,4206,4406,460
    GCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGR912VG914LD916IG918KV920WV922AD924DG926AA928VA930VW932TT934PE936SV938EA940GA942VF944AE946IG948PR950MA952EL954SG956SR958LA960AQ962QQ964ME966GL968LA9703742-6909PuroRPCR XZ0796,4806,5006,5206,5406,5606,5806,6006,6206,640
    GCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCEagIPfoIPH972RP974KE976PA978WF980LA982TV984GV986SP988DH990QG992KG994LG996SA998VV1000LP1002GV1004EA1006AE1008RA1010GV1012PA1014FL1016ET1018SA1020PR1022NL1024PF1026YE10283742-6909PuroRPCR XZ0796,6606,6806,7006,7206,7406,7606,7806,8006,820
    CGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGATAGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTATCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGSexAIRL1030GF1032TV1034TA1036DV1038EV1040PE1042GP1044RT1046WC1048MT1050RK1052PG1054A*10563742-6909EMCV IRES SitePuroRhomologPCRPCR XZ0796,8406,8606,8806,9006,9206,9406,9606,9807,000
    ATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAAAvrIIHindIIIEMCV IRES SitePCR XZ0797,0207,0407,0607,0807,1007,1207,1407,160
    GCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTPmlIPaqCIEMCV IRES SitePCR XZ0797,1807,2007,2207,2407,2607,2807,3007,3207,340
    GAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTaCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGggggagaaacccgggaccagggtCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCAtGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATTATACCGGTGTTGGTACcccctctttgggccctggtcccaKpnIPflMIAcc65IMG2EK4PG6TR8V7500-9467EMCV IRES SiteARRB2missinghomologho...gPCR XZ0797,3607,3807,4007,4207,4407,4607,4807,5007,520
    cttcaagaagtcgagccctaactgcaagctcaccgtgtacttgggcaagcgggacttcgtagatcacctggacaaagtggaccctgtagatggcgtggtgcttgtggaccctgactacctgaaggaccgcaaagtgtttgtgaccctcacctgcgccttccgctatggccgtgaagaagttcttcagctcgggattgacgttcgagtggcacatgaacccgttcgccctgaagcatctagtggacctgtttcacctgggacatctaccgcaccacgaacacctgggactgatggacttcctggcgtttcacaaacactgggagtggacgcggaaggcgataccggcacttPaqCIF10KK12SS14PN16CK18LT20VY22LG24KR26DF28VD30HL32DK34VD36PV38DG40VV42LV44DP46DY48LK50DR52KV54FV56TL58TC60AF62RY64GR66E7500-9467ARRB2missingPCR XZ0797,5407,5607,5807,6007,6207,6407,6607,6807,700
    gacctggatgtgctgggcttgtccttccgcaaagacctgttcatcgccacctaccaggccttccccccggtgcccaacccaccccggccccccacccgcctgcaggaccggctgctgaggaagctgggccagcatgcccaccccttcttcttcaccataccccagaatcttccatctggacctacacgacccgaacaggaaggcgtttctggacaagtagcggtggatggtccggaaggggggccacgggttgggtggggccggggggtgggcggacgtcctggccgacgactccttcgacccggtcgtacgggtggggaagaagaagtggtatggggtcttagaaggtaSbfIBbvCID68LD70VL72GL74SF76RK78DL80FI82AT84YQ86AF88PP90VP92NP94PR96PP98TR100LQ102DR104LL106RK108LG110QH112AH114PF116FF118TI120PQ122NL124P7500-9467ARRB2missingPCR XZ0797,7207,7407,7607,7807,8007,8207,8407,860
    gctccgtcacactgcagccaggcccagaggatacaggaaaggcctgcggcgtagactttgagattcgagccttctgtgctaaatcactagaagagaaaagccacaaaaggaactctgtgcggctggtgatccgaaaggtgcagttcgccccggagaaacccggcccccagccttccgaggcagtgtgacgtcggtccgggtctcctatgtcctttccggacgccgcatctgaaactctaagctcggaagacacgatttagtgatcttctcttttcggtgttttccttgagacacgccgaccactaggctttccacgtcaagcggggcctctttgggccgggggtcggaagAccIC126SV128TL130QP132GP134ED136TG138KA140CG142VD144FE146IR148AF150CA152KS154LE156EK158SH160KR162NS164VR166LV168IR170KV172QF174AP176EK178PG180PQ182PS1847500-9467ARRB2missingPCR XZ0797,8807,9007,9207,9407,9607,9808,0008,0208,040
    agccgaaaccacacgccacttcctcatgtctgaccggtccctgcacctcgaggcttccctggacaaggagctgtactaccatggggagcccctcaatgtaaatgtccacgtcaccaacaactccaccaagaccgtcaagaagatcaaagtctctgtgagacagtacgccgacatctcggctttggtgtgcggtgaaggagtacagactggccagggacgtggagctccgaagggacctgttcctcgacatgatggtacccctcggggagttacatttacaggtgcagtggttgttgaggtggttctggcagttcttctagtttcagagacactctgtcatgcggctgtagPspXIAE186TT188RH190FL192MS194DR196SL198HL200EA202SL204DK206EL208YY210HG212EP214LN216VN218VH220VT222NN224ST226KT228VK230KI232KV234SV236RQ238YA240DI2427500-9467ARRB2PCR XZ0798,0608,0808,1008,1208,1408,1608,1808,2008,220
    tgcctcttcagcaccgcccagtacaagtgtcctgtggctcaactcgaacaagatgaccaggtatctcccagctccacattctgtaaggtgtacaccataaccccactgctcagcgacaaccgggagaagcggggtctcgccctggatgggaaactcaagcacgaggacaccaaccacggagaagtcgtggcgggtcatgttcacaggacaccgagttgagcttgttctactggtccatagagggtcgaggtgtaagacattccacatgtggtattggggtgacgagtcgctgttggccctcttcgccccagagcgggacctaccctttgagttcgtgctcctgtggttggBsrGISexAICL244FS246TA248QY250KC252PV254AQ256LE258QD260DQ262VS264PS266ST268FC270KV272YT274IT276PL278LS280DN282RE284KR286GL288AL290DG292KL294KH296ED298TN3007500-9467ARRB2PCR XZ0798,2408,2608,2808,3008,3208,3408,3608,3808,400
    tggcttccagcaccatcgtgaaggagggtgccaacaaggaggtgctgggaatcctggtgtcctacagggtcaaggtgaagctggtggtgtctcgaggcggggatgtctctgtggagctgccttttgttcttatgcaccccaagccccacgaccacatccccctccccagaccccaaccgaaggtcgtggtagcacttcctcccacggttgttcctccacgacccttaggaccacaggatgtcccagttccacttcgaccaccacagagctccgcccctacagagacacctcgacggaaaacaagaatacgtggggttcggggtgctggtgtagggggaggggtctggggtAleILA302SS304TI306VK308EG310AN312KE314VL316GI318LV320SY322RV324KV326KL328VV330SR332GG334DV336SV338EL340PF342VL344MH346PK348PH350DH352IP354LP356RP358Q7500-9467ARRB2PCR XZ0798,4208,4408,4608,4808,5008,5208,5408,560
    gtcagccgctccggagacagatgtccctgtggacaccaacctcattgaatttgataccaactatgccacagatgatgacattgtgtttgaggactttgcccggcttcggctgaaggggatgaaggatgacgactatgatgatcaactctgcggcGGTGGTAGCGCTATGGCGAGCcagtcggcgaggcctctgtctacagggacacctgtggttggagtaacttaaactatggttgatacggtgtctactactgtaacacaaactcctgaaacgggccgaagccgacttcccctacttcctactgctgatactactagttgagacgccgCCACCATCGCGATACCGCTCGAfeIS360AA362PE364TD366VP368VD370TN372LI374EF376DT378NY380AT382DD384DI386VF388ED390FA392RL394RL396KG398MK400DD402DY404DD406QL408CG410GG412SA414MA416S7500-9467ARRB2homologMCPPCR XZ0798,5808,6008,6208,6408,6608,6808,7008,7208,740
    AACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAATTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTBsmBIEsp3IHincIIN418FT420QF422VL424VD426NG428GT430GD432VT434VA436PS438NF440AN442GI444AE446WI448SS450NS452RS454QA456YK458VT460CS462VR464QS466SA468QN470RK472YT474I7500-9467MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPPCR XZ0798,7608,7808,8008,8208,8408,8608,8808,9008,920
    AGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCXcmIAsiSIBsaBIK476VE478VP480KG482AW484RS486YL488NM490EL492TI494PI496FA498TN500SD502CE504LI506VK508AM510QG512LL514KD516GN518PI520PS522AI524AA526NS528GI530YG532GG5347500-9467from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPPCR XZ0798,9408,9608,9809,0009,0209,0409,0609,0809,100
    AACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCABsmBIEsp3ITG536GG538SM540AS542NF544TQ546FV548LV550DN552GG554TG556DV558TV560AP562SN564FA566NG568IA570EW572IS574SN576SR578SQ580AY582KV584TC586SV588RQ590SS5927500-9467from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPPCR XZ0799,1209,1409,1609,1809,2009,2209,2409,260
    GCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACAccIBstZ17IAQ594NR596KY598TI600KV602EV604PK606GA608WR610SY612LN614ME616LT618IP620IF622AT624NS626DC628EL630IV632KA634MQ636GL638LK640DG642NP644IP646SA648IA6507500-9467from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPPCR XZ0799,2809,3009,3209,3409,3609,3809,4009,4209,440
    CGAATAGCGGAATTTACTAAGAATTCCTCGAGCTAGAGCTcGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTGCTTATCGCCTTAAATGATTCTTAAGGAGCTCGATCTCGAgCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAEcoRIPspXI*1AN652SG654IY6567500-9467bGH poly(A) signalfrom ...dMCPMCPhomologPCR XZ0799,4609,4809,5009,5209,5409,5609,5809,6009,620
    CTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACGACGTCAGGTGGCACTTTTCgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTGCTGCAGTCCACCGTGAAAAGctatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttaEcoRVbGH poly(A) signal3' PB ITR longPCR XZ0799,6409,6609,6809,7009,7209,7409,7609,7809,800
    ataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgtattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgc3' PB ITR longPCR XZ0799,8209,8409,8609,8809,9009,9209,9409,960
    ctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaaNsiI3' PB ITR long3' PB ITR shortjt1010PCR XZ0799,98010,00010,02010,04010,06010,08010,10010,12010,140
    cccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcPCR XZ07910,16010,18010,20010,22010,24010,26010,28010,30010,320
    gacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcaPCR XZ07910,34010,36010,38010,40010,42010,44010,46010,48010,500
    gagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttctcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaPCR XZ07910,52010,54010,56010,58010,60010,62010,64010,660
    ccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtSapIBspQIPCR XZ07910,68010,70010,72010,74010,76010,78010,80010,82010,840
    gaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacccttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggPCR XZ07910,86010,88010,90010,92010,94010,96010,98011,00011,020
    aggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacctccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggPCR XZ07911,04011,06011,08011,10011,12011,14011,16011,18011,200
    ccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatctAlwNIPCR XZ07911,22011,24011,26011,28011,30011,32011,34011,360
    aggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaatcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttPCR XZ07911,38011,40011,42011,44011,46011,48011,50011,520
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    11525 bp8500900095001000010500110005001000150020002500PCR XZ079EF-1α promoterMCPPrimer 1primer from EF-1ajt1010Amp174-1031: Region 1174-1031: Region 29468-94707500-9467EKo152.2 (DNA)EKo273.2 (DNA)EF1a_hIgG1_R (DNA)XZp044.6 (DNA)XZp044.1 (DNA)XZp068.1 (DNA)XZp023.1 (DNA)XZp077.1 (DNA)YXo005.2 (DNA)XZp025.2 (DNA)oLSM0089 (DNA)2A1 (DNA)XZp050.3 (DNA)XZp079.5 (DNA)XZp050.4 (DNA)EKo454.4 (DNA)MCP-ADAR1-seq-F1 (DNA)EKo326.3 (DNA)oLSM0086 (DNA)AhdIFspIScaICspCIPsiIAloIFspIjt1009,5' PB ITR short5' PB ITR Longcore insul,RsrII,SrfISfiI,SpeIForward Target,target forward primer,+1Reverse Target,Target Reverse PrimerMfeISacIIoLSM0064 (DNA)XZp024.2 (DNA)homolog,oLSM0066 (DNA),+5XcmIBsaBI+3BsmBI,Esp3IMCP,XZp064.1 (DNA),XZp009.3 (DNA),AccI,+1MCP-ADAR-seq-F0 (DNA)homolog,XZp064.2 (DNA),XZp077.2 (DNA),+4XZ41-test-R1 (DNA)bGH poly(A) signalbghF (DNA)oLSM0085 (DNA),EcoRV3' PB ITR longNsiI3' PB ITR shortM13-48REV Genewiz (DNA)BspQI,SapIpUCori5F (DNA),pUCoriR (DNA)AlwNI

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    11525