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pCAGGS-PP1c-miRFP703
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgatcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacagctagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattSpeICAG promoter20406080100120140160
    cgccaatagggactttccattgacgtcaatgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctgataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacCAG promoter180200220240260280300320340
    gcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcgtcatgtagatgcataatcagtagcgataatggtacccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccgcgcgcggtccgccccBsaAISnaBICAG promoter360380400420440460480500520
    cggggcgggggcgagggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctgccccgcccccgctcccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcaacggaagcggggcacggggcgaCAG promoter540560580600620640660680700
    ccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcggcgcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgagcaaagaaaagacaccgacgcactttcggaatttcccgaggccctcccgggaaacacgAfeIApaIPfoIPspOMICAG promoter720740760780800820840860
    gggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcccccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgcacacgcgctcccctcgcgccggcccccgccacggggcgccacgcccccccgacgAfeIEagICAG promoter8809009209409609801,0001,0201,040
    gaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgccgccagcccgacattggggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcacgccccgcaccgcgccccgagcggcacggcccgBlpICAG promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggaaacagggtMreIPaqCISgrAICAG promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aatctggcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcgggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccatctccagcctcggggctgccgcagggggacggctgccttttagaccgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgcccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagaggtagaggtcggagccccgacggcgtccccctgccgacggaaCAG promoter1,4201,4401,4601,4801,5001,5201,5401,560
    cgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttgttgtgctgtctcatcattttggcaagaattcgccATGGCGGACGGGGAGCgccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatcgatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcgaggacccgttgcacgaccaacaacacgacagagtagtaaaaccgttcttaagcggTACCGCCTGCCCCTCGEcoRIMA2DG4E1735-2715CAG promoterCAG1640PPP1CB1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGAACGTGGACAGCCTCATCACCCGGCTGCTGGAGGTACGAGGATGTCGTCCAGGAAAGATTGTGCAGATGACTGAAGCAGAAGTTCGAGGCTTATGTATCAAGTCTCGGGAGATCTTTCTCAGCCAGCCTATTCTTTTGGAATTGGAAGCACCGCTGAAAATTTGTGGAGATATACTTGCACCTGTCGGAGTAGTGGGCCGACGACCTCCATGCTCCTACAGCAGGTCCTTTCTAACACGTCTACTGACTTCGTCTTCAAGCTCCGAATACATAGTTCAGAGCCCTCTAGAAAGAGTCGGTCGGATAAGAAAACCTTAACCTTCGTGGCGACTTTTAAACACCTCTATAPfoIBglIIL6NV8DS10LI12TR14LL16EV18RG20CR22PG24KI26VQ28MT30EA32EV34RG36LC38IK40SR42EI44FL46SQ48PI50LL52EL54EA56PL58KI60CG62DI641735-2715PPP1CB1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TCATGGACAGTATACAGATTTACTGAGATTATTTGAATATGGAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGGACAGAGGAAAGCAGTCTTTGGAAACCATTTGTTTGCTATTGGCTTATAAAATCAAATATCCAGAGAACTTCTTTCTCTTAAGAGGAAGTACCTGTCATATGTCTAAATGACTCTAATAAACTTATACCTCCAAAGGGTGGTCTTCGGTTGATAGAAAAGAATCCTCTAATACACCTGTCTCCTTTCGTCAGAAACCTTTGGTAAACAAACGATAACCGAATATTTTAGTTTATAGGTCTCTTGAAGAAAGAGAATTCTCCTAjuIPsiIAflIIBstZ17IHG66QY68TD70LL72RL74FE76YG78GF80PP82EA84NY86LF88LG90DY92VD94RG96KQ98SL100ET102IC104LL106LA108YK110IK112YP114EN116FF118LL120RG1221735-2715PPP1CB1,9401,9601,9802,0002,0202,0402,0602,0802,100
    AACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATGAATGCAAACGAAGATTTAATATTAAATTGTGGAAGACCTTCACTGATTGTTTTAACTGTCTGCCTATAGCAGCCATTGTGGATGAGAAGATCTTCTGTTGTCATGGAGGATTGTCACCAGACCTGCAATTTGGTACTCACACGATCGTAGTTAGCGTAAATACCTAAGATACTACTTACGTTTGCTTCTAAATTATAATTTAACACCTTCTGGAAGTGACTAACAAAATTGACAGACGGATATCGTCGGTAACACCTACTCTTCTAGAAGACAACAGTACCTCCTAACAGTGGTCTGGACGTTABbsIBmtINheISspIBglIINH124EC126AS128IN130RI132YG134FY136DE138CK140RR142FN144IK146LW148KT150FT152DC154FN156CL158PI160AA162IV164DE166KI168FC170CH172GG174LS176PD178LQ1801735-2715PPP1CB2,1202,1402,1602,1802,2002,2202,2402,260
    CTATGGAGCAGATTCGGAGAATTATGAGACCTACTGATGTCCCTGATACAGGTTTGCTCTGTGATTTGCTATGGTCTGATCCAGATAAGGATGTGCAAGGCTGGGGAGAAAATGATCGTGGTGTTTCCTTTACTTTTGGAGCTGATGTAGTCAGTAAATTTCTGAATCGTCATGAGATACCTCGTCTAAGCCTCTTAATACTCTGGATGACTACAGGGACTATGTCCAAACGAGACACTAAACGATACCAGACTAGGTCTATTCCTACACGTTCCGACCCCTCTTTTACTAGCACCACAAAGGAAATGAAAACCTCGACTACATCAGTCATTTAAAGACTTAGCAGTACTBsaIBseYIEcoNISM182EQ184IR186RI188MR190PT192DV194PD196TG198LL200CD202LL204WS206DP208DK210DV212QG214WG216EN218DR220GV222SF224TF226GA228DV230VS232KF234LN236RH238D1735-2715PPP1CB2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TTTAGATTTGATTTGTCGAGCTCATCAGGTGGTGGAAGATGGATATGAATTTTTTGCTAAACGACAGTTGGTAACCTTATTTTCAGCCCCAAATTACTGTGGCGAGTTTGATAATGCTGGTGGAATGATGAGTGTGGATGAAACTTTGATGTGTTCATTTCAGATATTGAAACCAAAATCTAAACTAAACAGCTCGAGTAGTCCACCACCTTCTACCTATACTTAAAAAACGATTTGCTGTCAACCATTGGAATAAAAGTCGGGGTTTAATGACACCGCTCAAACTATTACGACCACCTTACTACTCACACCTACTTTGAAACTACACAAGTAAAGTCTATAACTTTGGTSacIBstEIIEco53kIL240DL242IC244RA246HQ248VV250ED252GY254EF256FA258KR260QL262VT264LF266SA268PN270YC272GE274FD276NA278GG280MM282SV284DE286TL288MC290SF292QI294LK296P1735-2715PPP1CB2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TCTGAAAAGAAAGCTAAATACCAGTATGGTGGACTGAATTCTGGACGTCCTGTCACTCCACCTCGAACAGCTAATCCGCCGAAGAAAAGGggcggccgcatgGTAGCAGGTCATGCCTCTGGCAGCCCCGCATTCGGGACCGCCTCTCATTCGAATTGCGAACATGAAGAGATCCAGACTTTTCTTTCGATTTATGGTCATACCACCTGACTTAAGACCTGCAGGACAGTGAGGTGGAGCTTGTCGATTAGGCGGCTTCTTTTCCccgccggcgtacCATCGTCCAGTACGGAGACCGTCGGGGCGTAAGCCCTGGCGGAGAGTAAGCTTAACGCTTGTACTTCTCTAGGAhdIAloIEagINotIBstBIEcoRIS298EK300KA302KY304QY306GG308LN310SG312RP314VT316PP318RT320AN322PP324KK326R1735-2715GG2RM4VA6GH8AS10GS12PA14FG16TA18SH20SN22CE24HE26EI282716-3684PPP1CBmiRFP7032,6402,6602,6802,7002,7202,7402,7602,7802,800
    ACCTCGCCGGCTCGATCCAGCCGCATGGCGCGCTTCTGGTCGTCAGCGAACATGATCATCGCGTCATCCAGGCCAGCGCCAACGCCGCGGAATTTCTGAATCTCGGAAGCGTACTCGGCGTTCCGCTCGCCGAGATCGACGGCGATCTGTTGATCAAGATCCTGCCGCATCTCGATGGAGCGGCCGAGCTAGGTCGGCGTACCGCGCGAAGACCAGCAGTCGCTTGTACTAGTAGCGCAGTAGGTCCGGTCGCGGTTGCGGCGCCTTAAAGACTTAGAGCCTTCGCATGAGCCGCAAGGCGAGCGGCTCTAGCTGCCGCTAGACAACTAGTTCTAGGACGGCGTAGAGCTPsrIBsaBIHL30AG32SI34QP36HG38AL40LV42VS44EH46DH48RV50IQ52AS54AN56AA58EF60LN62LG64SV66LG68VP70LA72EI74DG76DL78LI80KI82LP84HL86D2716-3684miRFP7032,8202,8402,8602,8802,9002,9202,9402,960
    TCCCACCGCCGAAGGCATGCCGGTCGCGGTGCGCTGCCGGATCGGCAATCCCTCTACGGAGTACTGCGGTCTGATGCATCGGCCTCCGGAAGGCGGGCTGATCATCGAACTCGAACGTGCCGGCCCGTCGATCGATCTGTCAGGCACGCTGGCGCCGGCGCTGGAGCGGATCCGCAGGGTGGCGGCTTCCGTACGGCCAGCGCCACGCGACGGCCTAGCCGTTAGGGAGATGCCTCATGACGCCAGACTACGTAGCCGGAGGCCTTCCGCCCGACTAGTAGCTTGAGCTTGCACGGCCGGGCAGCTAGCTAGACAGTCCGTGCGACCGCGGCCGCGACCTCGCCTAGGCGClaIMreINsiIScaISphIBspDIBspEIEcoNISgrAIP88TA90EG92MP94VA96VR98CR100IG102NP104ST106EY108CG110LM112HR114PP116EG118GL120II122EL124ER126AG128PS130ID132LS134GT136LA138PA140LE142RI144R2716-3684miRFP7032,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACGGCGGGTTCACTGCGCGCGCTGTGCGATGACACCGTGCTGCTGTTTCAGCAGTGCACCGGCTACGACCGGGTGATGGTGTATCGTTTCGATGAGCAAGGCCACGGCCTGGTATTCTCCGAGTGCCATGTGCCTGGGCTCGAATCCTATTTCGGCAACCGCTATCCGTCGTCGCTGCCGCCCAAGTGACGCGCGCGACACGCTACTGTGGCACGACGACAAAGTCGTCACGTGGCCGATGCTGGCCCACTACCACATAGCAAAGCTACTCGTTCCGGTGCCGGACCATAAGAGGCTCACGGTACACGGACCCGAGCTTAGGATAAAGCCGTTGGCGATAGGCAGCAGCGPflMIT146AG148SL150RA152LC154DD156TV158LL160FQ162QC164TG166YD168RV170MV172YR174FD176EQ178GH180GL182VF184SE186CH188VP190GL192ES194YF196GN198RY200PS202S2716-3684miRFP7033,1603,1803,2003,2203,2403,2603,2803,3003,320
    TGGTCCCGCAGATGGCGCGGCAGCTGTACGTGCGGCAGCGCGTCCGCGTGCTGGTCGACGTCACCTATCAGCCGGTGCCGCTGGAGCCGCGGCTGTCGCCGCTGACCGGGCGCGATCTCGACATGTCGGGCTGCTTCCTGCGCTCGATGTCGCCGATCCATCTGCAGTTCCTGAAACCAGGGCGTCTACCGCGCCGTCGACATGCACGCCGTCGCGCAGGCGCACGACCAGCTGCAGTGGATAGTCGGCCACGGCGACCTCGGCGCCGACAGCGGCGACTGGCCCGCGCTAGAGCTGTACAGCCCGACGAAGGACGCGAGCTACAGCGGCTAGGTAGACGTCAAGGACTTPstISalIBsaAIPvuIIHincIIL204VP206QM208AR210QL212YV214RQ216RV218RV220LV222DV224TY226QP228VP230LE232PR234LS236PL238TG240RD242LD244MS246GC248FL250RS252MS254PI256HL258QF260LK2622716-3684miRFP7033,3403,3603,3803,4003,4203,4403,4603,4803,500
    GGACATGGGCGTGCGCGCCACCCTGGCGGTGTCGCTGGTGGTCGGCGGCAAGCTGTGGGGCCTGGTTGTCTGTCACCATTATCTGCCGCGCTTCATCCGTTTCGAGCTGCGGGCGATCTGCAAACGGCTCGCCGAAAGGATCGCGACGCGGATCACCGCGCTTGAGAGCTctagaCCTGTACCCGCACGCGCGGTGGGACCGCCACAGCGACCACCAGCCGCCGTTCGACACCCCGGACCAACAGACAGTGGTAATAGACGGCGCGAAGTAGGCAAAGCTCGACGCCCGCTAGACGTTTGCCGAGCGGCTTTCCTAGCGCTGCGCCTAGTGGCGCGAACTCTCGAgatctNruISacIXbaIEco53kIDM264GV266RA268TL270AV272SL274VV276GG278KL280WG282LV284VC286HH288YL290PR292FI294RF296EL298RA300IC302KR304LA306ER308IA310TR312IT314AL316ES318SR3202716-3684miRFP7033,5203,5403,5603,5803,6003,6203,6403,660
    TAAgtcgacggatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagATTcagctgcctagaaaaagggagacggtttttaatacccctgtagtacttcggggaactcgtagactgaagaccgattatttcctttaaataaaagtaacgttatcacacaaccttaaaaaacacagagagtgagccttcctgtataccctcccgtttagtaaattttgtagtcAjuISalIHincII*V322DRabbit b-globin 3'-flanking sequenceCAGGS-R23,6803,7003,7203,7403,7603,7803,8003,8203,840
    aatgagtatttggtttagagtttggcaacatatgccatatgctggctgccatgaacaaaggtggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagatttttttttatattttgtttgtgttatttttactcataaaccaaatctcaaaccgttgtatacggtatacgaccgacggtacttgtttccaccgatatttctccagtagtcatatactttgtcgggggacgacaggtaaggaataaggtatcttttcggaactgaactccaatctaaaaaaaaatataaaacaaacacaataaaPflMIRabbit b-globin 3'-flanking sequence3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatgaagatccctcgacctgcagcccaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgaaaagaaattgtagggattttaaaaggaatgtacaaaatgatcggtctaaaaaggaggagaggactgatgagggtcagtatcgacagggagaagagaatacttctagggagctggacgtcgggttcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcPstIHindIIIRabbit b-globin 3'-flanking sequenceM13-revLacO4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatctcaattagtcagcaaccatagtcccgcccctgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgcctaggcgtagagttaatcagtcgttggtatcagggcggggaBsaBISV40 Orilac promoterLacO4,2204,2404,2604,2804,3004,3204,3404,360
    aactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgattgaacaaatBplIBplISfiIStuIAvrIISV40 Ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ttgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattaacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctaggcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataaPsiISV40 Ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcSapIBspQI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcgDrdIBciVIBssSIBssSαIColE1 origin4,9204,9404,9604,9805,0005,0205,0405,060
    ctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttagaaagagggaagcccttcgcaccgcgaaagagttacgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatBseYIColE1 origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccgagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggcColE1 origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcaccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtgColE1 origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggaggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcAhdIAmpRAmp_R5,6205,6405,6605,6805,7005,7205,7405,760
    ggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaaBsaIAmpRAmp_R5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcaggcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcAclIFspIAmpRAmp_R5,9605,9806,0006,0206,0406,0606,0806,1006,120
    aagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataatttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattaScaIAmpRAmp_R6,1406,1606,1806,2006,2206,2406,2606,2806,300
    accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacagtggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtcAclIBssSIBssSαIAmp_RAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    gaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgcttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcSspIBciVIAmp_R6,4806,5006,5206,5406,5606,5806,6006,6206,640
    aaaagtgccacctgttttcacggtggacSexAI6,660
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    6664 bp450050005500600050010001500CAG promoterCAG1640AmpRseqr_miRFP (DNA)

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    6664