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pDF0118_Twinstrep-SUMO
  • Sequence Map
    Editing disabled on read-only sequence.
    TCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAori20406080100120140160
    TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACori180200220240260280300320340
    GACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCori360380400420440460480500520
    GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGori540560580600620640660680700
    CTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTACGCCATAAAAGAGGAATGCPciISapIBspQI720740760780800820840860
    CATCTGTGCGGTATTTCACACCGCAATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGTAGACACGCCATAAAGTGTGGCGTTACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGATGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGPflFIBstZ17ITth111I8809009209409609801,0001,0201,040
    GCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTACGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGACGCCATTTCGAGTAGTCGCACCAGCACTTCGCTAAGTGTCTACAGACGGACAAGTAGGCGCAGGTCGAGCAACTCAAAGAGGTCTTCGCAATBsmBIEsp3IROPROPpGEX_3_primer1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTACAGACCGAAGACTATTTCGCCCGGTACAATTCCCGCCAAAAAAGGACAAACCAGTGACTACGGAGGCACATTCCCCCTAAAGACAAGTACCCCCATTACTATGGCTACTTTGCTCTCTCCTACGAGTGCTATGCCCAATGACTACTACTTGTACGGGCCAATGACCTTGCAACROPROP1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTACTCCCATTTGTTGACCGCCATACCTACGCCGCCCTGGTCTCTTTTTAGTGAGTCCCAGTTACGGTCGCGAAGCAATTATGTCTACATCCACAAGGTGTCCCATCGGTCGTCGTAGGACGCTACGTCTAGGCCTTGTATTACCACGTCCCGCGACTGAAGGCGCAAAGGTCTGAA1,4201,4401,4601,4801,5001,5201,5401,560
    TACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGATGCTTTGTGCCTTTGGCTTCTGGTAAGTACAACAACGAGTCCAGCGTCTGCAAAACGTCGTCGTCAGCGAAGTGCAAGCGAGCGCATAGCCACTAAGTAAGACGATTGGTCATTCCGTTGGGGCGGTCGGATCGGCCCAGGAGTTGCTGTCCTCGTGCTAGTACGCGTGGGCACFspAI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    GCCAGGACCCAACGCTGCCCGAGATGCGCCGCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTCTGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGCGGTCCTGGGTTGCGACGGGCTCTACGCGGCGCACGCCGACGACCTCTACCGCCTGCGCTACCTATACAAGACGGTTCCCAACCAAACGCGTAAGTGTCAAGAGGCGTTCTTAACTAACCGAGGTTAAGAACCTCACCACTTAGGCAATCGCTCCACGGCGGCCGAAGGTAAGTC1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCAGCTCCACCGGGCCGAGGTACGTGGCGCTGCGTTGCGCCCCTCCGTCTGTTCCATATCCCGCCGCGGATGTTAGGTACGGTTGGGCAAGGTACACGAGCGGCTCCGCCGTATTTAGCGGCACTGCTAGTCGCCAGGTTACTAGCTTCAATCCGACCATTCTCGGCGCTCGCTAGtet (576 - 1190)1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACGCCAGCAAGACGTAGCCCAGCGCGTCGGCCGCCATGCCGGCGATAATGAACTTCGACAGGGACTACCAGCAGTAGATGGACGGACCTGTCGTACCGGACGTTGCGCCCGTAGGGCTACGGCGGCCTTCGCTCTTCTTAGTATTACCCCTTCCGGTAGGTCGGAGCGCAGCGCTTGCGGTCGTTCTGCATCGGGTCGCGCAGCCGGCGGTACGGCCGCTATTANruIBstAPItet (576 - 1190)2,1202,1402,1602,1802,2002,2202,2402,260
    GGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGCCGGACGAAGAGCGGCTTTGCAAACCACCGCCCTGGTCACTGCTTCCGAACTCGCTCCCGCACGTTCTAAGGCTTATGGCGTTCGCTGTCCGGCTAGTAGCAGCGCGAGGTCGCTTTCGCCAGGAGCGGCTTTTACTGGGTCTCGCGACGGCCGTGGACAGGATGCTCAACGTACtet (576 - 1190)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTTATTTCTTCTGTCAGTATTCACGCCGCTGCTATCAGTACGGGGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGGGCCACGGATTACTCACTCGATTGAATGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAtet (576 - 1190)lacI2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGTCGTCCGCTTBsmBIEsp3IlacI2,6402,6602,6802,7002,7202,7402,7602,7802,800
    AATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCTTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGACAGAAGCCATAGCAGCATAGGGTGATGGCTCTATAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACGGGAGHpaIEcoRVlacI2,8202,8402,8602,8802,9002,9202,9402,960
    ATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTAApaIPspOMIlacI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCCGCTGGTCTACGAGGTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGCGMluIlacI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGCAACGCGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGAClacI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    TTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCAAACGGGCGGTCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTCTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAAAGBstAPIlacI3,5203,5403,5603,5803,6003,6203,6403,660
    ACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTTGTAAGTGGTGGGACTTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGGGTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCAEcoNItet (300 - 563)lacI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCCGTACGTTCCTCTACCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGAGTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGGTCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGSphItet (300 - 563)pBRrevB...primer3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCAGGCCGCATCTCCTAGCTCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATTGTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGGTACCCGTCGTCGGTAGTAGTAGTAGTAGTGTCGTCGCCGGACCACGGCGCBarINcoIXbaIMG2SS4HH6HH8HH10SS12GL14VP16R4151-9406Translation 4151-94066x His tag/ Twin StreplacOFP0234LacOT7T7_promoter6xHIST7_tr..._RBS4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CGGCAGCCATATGGCTAGCTGGAGCCATCCGCAGTTTGAAAAAGGTGGTGGTAGCGGTGGTGGTTCAGGTGGTAGTGCATGGTCACACCCTCAGTTTGAGAAAATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCGCCGTCGGTATACCGATCGACCTCGGTAGGCGTCAAACTTTTTCCACCACCATCGCCACCACCAAGTCCACCATCACGTACCAGTGTGGGAGTCAAACTCTTTTACAGCCTGAGTCTTCAGTTAGTTCTTCGATTCGGTCTCCAGTTCGGTCTTCAGTTCGGACTCTGAGTGTAGBmtINdeINheIG18SH20MA22SW24SH26PQ28FE30KG32GG34SG36GG38SG40GS42AW44SH46PQ48FE50KM52SD54SE56VN58QE60AK62PE64VK66PE68VK70PE72TH74I4151-9406Translation 4151-94066x His tag/ Twin StrepSumoStrep Tag4,2204,2404,2604,2804,3004,3204,3404,360
    AATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCaAGATCAAAAAGAccACTCCTTTaAGAaggCTGATggAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATCCAAGCTGATCAGACCCCTGAAGATTTGGACATTAAATTTCCACAGGCTACCTAGAAGTCTCTAGAAGAAGtTCTAGTTTTTCTggTGAGGAAAtTCTtccGACTAccTTCGCAAGCGATTTTCTGTCCCATTCCTTTACCTGAGGAATTCTAAGAACATGCTGCCATAATCTTAGGTTCGACTAGTCTGGGGACTTCTAAACCTGTAflIIN76LK78VS80DG82SS84EI86FF88KI90KK92TT94PL96RR98LM100EA102FA104KR106QG108KE110MD112SL114RF116LY118DG120IR122IQ124AD126QT128PE130DL132D4151-9406Translation 4151-9406Sumo4,3804,4004,4204,4404,4604,4804,5004,5204,540
    TGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCATGACCACTACCATGAAGATCAGCATCGAGTTCTTGGAGCCCTTCCGCATGACGAAGTGGCAGGAGTCCACGCGCCGCAATAAGAACAATAAAGAGTTTGTGCGTGGCCAAGCGTTTGCCCGTTGACCTCCTATTGCTATAATAACTCCGAGTGTCTCTTGTCTAACCACCTAGGTACTGGTGATGGTACTTCTAGTCGTAGCTCAAGAACCTCGGGAAGGCGTACTGCTTCACCGTCCTCAGGTGCGCGGCGTTATTCTTGTTATTTCTCAAACACGCACCGGTTCGCAAACGGGCAACBamHIMT2TT4MK6IS8IE10FL12EP14FR16MT18KW20QE22ST24RR26NK28NN30KE32FV34RG36QA38FA40RW424601-9406M134ED136ND138II140EA142HR144EQ146IG148GS150MT152TT154MK156IS158IE160FL162EP164FR166MT168KW170QE172ST174RR176NK178NN180KE182FV184RG186QA188FA190RW1924151-9406MT2TT4MK6IS8IE10FL12EP14FR16MT18KW20QE22ST24RR26NK28NN30KE32FV34RG36QA38FA40RW424601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-9406SumoForwardgRAMP processing fwd4,5604,5804,6004,6204,6404,6604,6804,7004,720
    GcacCGCAACAAGAAAGATAACACCAAGGGTCGTCCGtacATTACTGGTACGCTCCTGCGCTCAGCGGTGATTCGCTCCGCGgagAACCTGCTGACACTGAGTGACGGTAAGATCTCCGAGAAAACCTGCTGCCCGGGGAAATTCGACACGGAAGATAAGGACCGTTTACTCCAGCgtgGCGTTGTTCTTTCTATTGTGGTTCCCAGCAGGCatgTAATGACCATGCGAGGACGCGAGTCGCCACTAAGCGAGGCGCctcTTGGACGACTGTGACTCACTGCCATTCTAGAGGCTCTTTTGGACGACGGGCCCCTTTAAGCTGTGCCTTCTATTCCTGGCAAATGAGGTCSmaIXmaITspMIDraIIIHR44NK46KD48NT50KG52RP54YI56TG58TL60LR62SA64VI66RS68AE70NL72LT74LS76DG78KI80SE82KT84CC86PG88KF90DT92ED94KD96RL98LQ1004601-9406HR194NK196KD198NT200KG202RP204YI206TG208TL210LR212SA214VI216RS218AE220NL222LT224LS226DG228KI230SE232KT234CC236PG238KF240DT242ED244KD246RL248LQ2504151-9406HR44NK46KD48NT50KG52RP54YI56TG58TL60LR62SA64VI66RS68AE70NL72LT74LS76DG78KI80SE82KT84CC86PG88KF90DT92ED94KD96RL98LQ1004601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94064,7404,7604,7804,8004,8204,8404,8604,8804,900
    CTGCGTCAACGCTCCACGCTGCGTTGGACAGACAAGAATCCGTGTCCCGACAATGCGGAAACGTATTGTCCGTTCTGTGAGCTCCTCGGCCGCTCCGGAAATGACGGTAAGAAAGCAGAAAAGAAGgacTGGCGCTTCCGCATCCATTTTGGTaacCTTTCCCTGCCGGGCAAGCGACGCAGTTGCGAGGTGCGACGCAACCTGTCTGTTCTTAGGCACAGGGCTGTTACGCCTTTGCATAACAGGCAAGACACTCGAGGAGCCGGCGAGGCCTTTACTGCCATTCTTTCGTCTTTTCTTCctgACCGCGAAGGCGTAGGTAAAACCAttgGAAAGGGACGGCCCGTTCGPsrISacIEco53kILR102QR104ST106LR108WT110DK112NP114CP116DN118AE120TY122CP124FC126EL128LG130RS132GN134DG136KK138AE140KK142DW144RF146RI148HF150GN152LS154LP156GK1584601-9406LR252QR254ST256LR258WT260DK262NP264CP266DN268AE270TY272CP274FC276EL278LG280RS282GN284DG286KK288AE290KK292DW294RF296RI298HF300GN302LS304LP306GK3084151-9406LR102QR104ST106LR108WT110DK112NP114CP116DN118AE120TY122CP124FC126EL128LG130RS132GN134DG136KK138AE140KK142DW144RF146RI148HF150GN152LS154LP156GK1584601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94064,9204,9404,9604,9805,0005,0205,0405,060
    CCGACTTCGACGGTCCGAAGGCAATTGGTTCACAGCGCGTATTAAATCGTGTGGATTTTAAGTCGGGAAAGGCACATGACTTCTTCAAGGCTTATGAGGTGGACCATACCCGCTTCCCTCGCTTTGAAGGGGAGATCACCATCGACAATAAAGTGTCCGCCGAGGCACGTAAGTTGGCTGAAGCTGCCAGGCTTCCGTTAACCAAGTGTCGCGCATAATTTAGCACACCTAAAATTCAGCCCTTTCCGTGTACTGAAGAAGTTCCGAATACTCCACCTGGTATGGGCGAAGGGAGCGAAACTTCCCCTCTAGTGGTAGCTGTTATTTCACAGGCGGCTCCGTGCATTCAAMfeIRsrIIPD160FD162GP164KA166IG168SQ170RV172LN174RV176DF178KS180GK182AH184DF186FK188AY190EV192DH194TR196FP198RF200EG202EI204TI206DN208KV210SA212EA214RK216L4601-9406PD310FD312GP314KA316IG318SQ320RV322LN324RV326DF328KS330GK332AH334DF336FK338AY340EV342DH344TR346FP348RF350EG352EI354TI356DN358KV360SA362EA364RK366L4151-9406PD160FD162GP164KA166IG168SQ170RV172LN174RV176DF178KS180GK182AH184DF186FK188AY190EV192DH194TR196FP198RF200EG202EI204TI206DN208KV210SA212EA214RK216L4601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,0805,1005,1205,1405,1605,1805,2005,2205,240
    GCTGTGTGACAGCCTTAAATTCACGGATCGCCTGTGTGGAGCCCTGTGCGTCATCCGCTTCGACGAGTATACACCGGCCGCGGACAGCGGTAAGCAAACCGAGAATGTGCAGGCAGAGCCCAACGCAAACCTGGCGGAGAAAACTGCCGAGCAAATTATCTCGATCCTCGATGACCGACACACTGTCGGAATTTAAGTGCCTAGCGGACACACCTCGGGACACGCAGTAGGCGAAGCTGCTCATATGTGGCCGGCGCCTGTCGCCATTCGTTTGGCTCTTACACGTCCGTCTCGGGTTGCGTTTGGACCGCCTCTTTTGACGGCTCGTTTAATAGAGCTAGGAGCTACTGBstZ17IL218CD220SL222KF224TD226RL228CG230AL232CV234IR236FD238EY240TP242AA244DS246GK248QT250EN252VQ254AE256PN258AN260LA262EK264TA266EQ268II270SI272LD274D4601-9406L368CD370SL372KF374TD376RL378CG380AL382CV384IR386FD388EY390TP392AA394DS396GK398QT400EN402VQ404AE406PN408AN410LA412EK414TA416EQ418II420SI422LD424D4151-9406L218CD220SL222KF224TD226RL228CG230AL232CV234IR236FD238EY240TP242AA244DS246GK248QT250EN252VQ254AE256PN258AN260LA262EK264TA266EQ268II270SI272LD274D4601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,2605,2805,3005,3205,3405,3605,3805,4005,420
    AACAAGAAAACGGAATACACCCGCCTTTTAGCGGACGCCATCCGCTCATTGCGTCGCTCCTCAAAACTTGTTGCAGGTTTGCCCAAGGATCACGATGGTAAGGACGATCATTACTTGtggGACATTGGGAAGAAGAAGAAGGATGAGAATTCGGTTACCATCCGTCAGATTCTTATTGTTCTTTTGCCTTATGTGGGCGGAAAATCGCCTGCGGTAGGCGAGTAACGCAGCGAGGAGTTTTGAACAACGTCCAAACGGGTTCCTAGTGCTACCATTCCTGCTAGTAATGAACaccCTGTAACCCTTCTTCTTCTTCCTACTCTTAAGCCAATGGTAGGCAGTCTAAGAATEcoRIN276KK278TE280YT282RL284LA286DA288IR290SL292RR294SS296KL298VA300GL302PK304DH306DG308KD310DH312YL314WD316IG318KK320KK322DE324NS326VT328IR330QI332L4601-9406N426KK428TE430YT432RL434LA436DA438IR440SL442RR444SS446KL448VA450GL452PK454DH456DG458KD460DH462YL464WD466IG468KK470KK472DE474NS476VT478IR480QI482L4151-9406N276KK278TE280YT282RL284LA286DA288IR290SL292RR294SS296KL298VA300GL302PK304DH306DG308KD310DH312YL314WD316IG318KK320KK322DE324NS326VT328IR330QI332L4601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,4405,4605,4805,5005,5205,5405,5605,5805,600
    CCACGAGTGCCGACACGAAGGAGCTGAAGAACGCTGGTAAGTGGCGCGAGTTTTGTGAGAAGCTTGGTGAGGCGCTTTATTTGAAGAGTAAGGATATGAGCGGCGGGTTGAAGATTACACGCCGTATTCTCGGGGACGCAGAGTTTCATGGCAAGCCAGATCGCTTGGAAAAGTCGGTGCTCACGGCTGTGCTTCCTCGACTTCTTGCGACCATTCACCGCGCTCAAAACACTCTTCGAACCACTCCGCGAAATAAACTTCTCATTCCTATACTCGCCGCCCAACTTCTAATGTGCGGCATAAGAGCCCCTGCGTCTCAAAGTACCGTTCGGTCTAGCGAACCTTTTCAGT334TS336AD338TK340EL342KN344AG346KW348RE350FC352EK354LG356EA358LY360LK362SK364DM366SG368GL370KI372TR374RI376LG378DA380EF382HG384KP386DR388LE390KS3924601-9406T484TS486AD488TK490EL492KN494AG496KW498RE500FC502EK504LG506EA508LY510LK512SK514DM516SG518GL520KI522TR524RI526LG528DA530EF532HG534KP536DR538LE540KS5424151-9406T334TS336AD338TK340EL342KN344AG346KW348RE350FC352EK354LG356EA358LY360LK362SK364DM366SG368GL370KI372TR374RI376LG378DA380EF382HG384KP386DR388LE390KS3924601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,6205,6405,6605,6805,7005,7205,7405,760
    CCGTAGTGTCTCGATCGGAAGCGTGTTAAAGGAAACCGTGGTGTGCGGAGAACTCGTCGCAAAGACCCCGTTCTTCTTTGGCGCGATCGACGAGGATGCAAAACAAACTgacTTACAGGTACTGCTTACTCCGGACAATAAGTATCGCCTGCCGCGCAGCGCCGTCCGCGGCATCGGCATCACAGAGCTAGCCTTCGCACAATTTCCTTTGGCACCACACGCCTCTTGAGCAGCGTTTCTGGGGCAAGAAGAAACCGCGCTAGCTGCTCCTACGTTTTGTTTGActgAATGTCCATGACGAATGAGGCCTGTTATTCATAGCGGACGGCGCGTCGCGGCAGGCGCCGTAGRS394VS396IG398SV400LK402ET404VV406CG408EL410VA412KT414PF416FF418GA420ID422ED424AK426QT428DL430QV432LL434TP436DN438KY440RL442PR444SA446VR448GI4504601-9406RS544VS546IG548SV550LK552ET554VV556CG558EL560VA562KT564PF566FF568GA570ID572ED574AK576QT578DL580QV582LL584TP586DN588KY590RL592PR594SA596VR598GI6004151-9406RS394VS396IG398SV400LK402ET404VV406CG408EL410VA412KT414PF416FF418GA420ID422ED424AK426QT428DL430QV432LL434TP436DN438KY440RL442PR444SA446VR448GI4504601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,7805,8005,8205,8405,8605,8805,9005,9205,940
    CTTCGCCGCGATCTTCAAACGTACTTTGACAGTCCCTGTAATGCGGAGTTAGGCGGTCGTCCATGTATGTGCAAGACCTGTCGTATCATGCGTGGCATTACGGTTATGgacGCGCGCTCCGAGTATAACGCGCCCCCGGAGATTCGTCACCGTACCCGCATTAATCCGTTTACGGGAAGCGGCGCTAGAAGTTTGCATGAAACTGTCAGGGACATTACGCCTCAATCCGCCAGCAGGTACATACACGTTCTGGACAGCATAGTACGCACCGTAATGCCAATACctgCGCGCGAGGCTCATATTGCGCGGGGGCCTCTAAGCAGTGGCATGGGCGTAATTAGGCAAATGCCLR452RD454LQ456TY458FD460SP462CN464AE466LG468GR470PC472MC474KT476CR478IM480RG482IT484VM486DA488RS490EY492NA494PP496EI498RH500RT502RI504NP506FT5084601-9406LR602RD604LQ606TY608FD610SP612CN614AE616LG618GR620PC622MC624KT626CR628IM630RG632IT634VM636DA638RS640EY642NA644PP646EI648RH650RT652RI654NP656FT6584151-9406LR452RD454LQ456TY458FD460SP462CN464AE466LG468GR470PC472MC474KT476CR478IM480RG482IT484VM486DA488RS490EY492NA494PP496EI498RH500RT502RI504NP506FT5084601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,9605,9806,0006,0206,0406,0606,0806,1006,120
    GAACAGTTGCTGAGGGCGCCCTGTTTAATATGGAAGTGGCCCCGGAAGGGATCGTATTCCCATTCCAATTACGCTATCGCGGCTCTGAGGACGGGCTCCCAGACGCGCTCAAGACCGTGCTGAAGTGGTGGGCAGAGGGGCAAGCTTTTATGTCAGGGGCCGCCAGTACCGGGAACTTGTCAACGACTCCCGCGGGACAAATTATACCTTCACCGGGGCCTTCCCTAGCATAAGGGTAAGGTTAATGCGATAGCGCCGAGACTCCTGCCCGAGGGTCTGCGCGAGTTCTGGCACGACTTCACCACCCGTCTCCCCGTTCGAAAATACAGTCCCCGGCGGTCATGGCCCTTBbvCIGT510VA512EG514AL516FN518ME520VA522PE524GI526VF528PF530QL532RY534RG536SE538DG540LP542DA544LK546TV548LK550WW552AE554GQ556AF558MS560GA562AS564TG566K4601-9406GT660VA662EG664AL666FN668ME670VA672PE674GI676VF678PF680QL682RY684RG686SE688DG690LP692DA694LK696TV698LK700WW702AE704GQ706AF708MS710GA712AS714TG716K4151-9406GT510VA512EG514AL516FN518ME520VA522PE524GI526VF528PF530QL532RY534RG536SE538DG540LP542DA544LK546TV548LK550WW552AE554GQ556AF558MS560GA562AS564TG566K4601-9403Translation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94066,1406,1606,1806,2006,2206,2406,2606,2806,300
    AGGCCGTTTTCGTATGGAGAATGCCAAATATGAAACCTTGGATCTGAGCGATGAGAATCAACGCAATGACTACTTGAAGAATTGGGGCTGGCGTGACGAGAAGGGTCTTGAGGAGCTTAAGAAACGTTTAAATTCAGGTCTGCCGGAGCCTGGCAATTACCGTGACCCGAAGTGGTCCGGCAAAAGCATACCTCTTACGGTTTATACTTTGGAACCTAGACTCGCTACTCTTAGTTGCGTTACTGATGAACTTCTTAACCCCGACCGCACTGCTCTTCCCAGAACTCCTCGAATTCTTTGCAAATTTAAGTCCAGACGGCCTCGGACCGTTAATGGCACTGGGCTTCACCAflIIG568RF570RM572EN574AK576YE578TL580DL582SD584EN586QR588ND590YL592KN594WG596WR598DE600KG602LE604EL606KK608RL610NS612GL614PE616PG618NY620RD622PK624W4601-9406G718RF720RM722EN724AK726YE728TL730DL732SD734EN736QR738ND740YL742KN744WG746WR748DE750KG752LE754EL756KK758RL760NS762GL764PE766PG768NY770RD772PK774W4151-9406G568RF570RM572EN574AK576YE578TL580DL582SD584EN586QR588ND590YL592KN594WG596WR598DE600KG602LE604EL606KK608RL610NS612GL614PE616PG618NY620RD622PK624W4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 1Twist piece 26,3206,3406,3606,3806,4006,4206,4406,460
    CATGAAATCAACGTGTCAATCGAGATGGCGAGTCCCTTCATCAACGGCgacCCTATCCGCGCAGCTGTAGACAAGCGTGGGACGgacGTCGTGACCTTCGTTAAGTACAAGGCGGAAGGGGAAGAAGCAAAGCCCGTGTGCGCATATAAGGCGGAGTCGTTTCGCGGCGTCATTCGTACTTTAGTTGCACAGTTAGCTCTACCGCTCAGGGAAGTAGTTGCCGctgGGATAGGCGCGTCGACATCTGTTCGCACCCTGCctgCAGCACTGGAAGCAATTCATGTTCCGCCTTCCCCTTCTTCGTTTCGGGCACACGCGTATATTCCGCCTCAGCAAAGCGCCGCAGTAAGZraIAatIIFspAIH626EI628NV630SI632EM634AS636PF638IN640GD642PI644RA646AV648DK650RG652TD654VV656TF658VK660YK662AE664GE666EA668KP670VC672AY674KA676ES678FR680GV682I4601-9406H776EI778NV780SI782EM784AS786PF788IN790GD792PI794RA796AV798DK800RG802TD804VV806TF808VK810YK812AE814GE816EA818KP820VC822AY824KA826ES828FR830GV832I4151-9406H626EI628NV630SI632EM634AS636PF638IN640GD642PI644RA646AV648DK650RG652TD654VV656TF658VK660YK662AE664GE666EA668KP670VC672AY674KA676ES678FR680GV682I4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 26,4806,5006,5206,5406,5606,5806,6006,6206,640
    GTTCGGCTGTAGCACGCATTCACATGGAAGATGGAGTGCCCCTGACTGAGCTTACTCACTCAGATTGCGAGTGTTTGCTCTGTCAAATCTTCGGGTCGGAATATGAGGCTGGTAAGATTCGTTTCGAAgatTTAGTATTTGAGAGTgacCCGGAGCCGGTCACCTTCGATCACGTCAAGCCGACATCGTGCGTAAGTGTACCTTCTACCTCACGGGGACTGACTCGAATGAGTGAGTCTAACGCTCACAAACGAGACAGTTTAGAAGCCCAGCCTTATACTCCGACCATTCTAAGCAAAGCTTctaAATCATAAACTCTCActgGGCCTCGGCCAGTGGAAGCTAGTGCAR684SA686VA688RI690HM692ED694GV696PL698TE700LT702HS704DC706EC708LL710CQ712IF714GS716EY718EA720GK722IR724FE726DL728VF730ES732DP734EP736VT738FD740HV7424601-9406R834SA836VA838RI840HM842ED844GV846PL848TE850LT852HS854DC856EC858LL860CQ862IF864GS866EY868EA870GK872IR874FE876DL878VF880ES882DP884EP886VT888FD890HV8924151-9406R684SA686VA688RI690HM692ED694GV696PL698TE700LT702HS704DC706EC708LL710CQ712IF714GS716EY718EA720GK722IR724FE726DL728VF730ES732DP734EP736VT738FD740HV7424601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 26,6606,6806,7006,7206,7406,7606,7806,8006,820
    TGCAATCgacCGCTTCACCGGCGGTGCCGCTGACAAGAAGAAGTTCGACGACAGTCCTTTGCCAGGGTCGCCGGCGCGCCCTCTGATGCTGAAGGGTTCCTTCTGGATTCGCCGTGACGTTCTCGAAGATGAGGAGTATTGCAAAGCGCTCGGGAAAGCATTAGCCGATGTAAATACGTTAGctgGCGAAGTGGCCGCCACGGCGACTGTTCTTCTTCAAGCTGCTGTCAGGAAACGGTCCCAGCGGCCGCGCGGGAGACTACGACTTCCCAAGGAAGACCTAAGCGGCACTGCAAGAGCTTCTACTCCTCATAACGTTTCGCGAGCCCTTTCGTAATCGGCTACATTTAAscIMreIEcoNIAI744DR746FT748GG750AA752DK754KK756FD758DS760PL762PG764SP766AR768PL770ML772KG774SF776WI778RR780DV782LE784DE786EY788CK790AL792GK794AL796AD798VN8004601-9406AI894DR896FT898GG900AA902DK904KK906FD908DS910PL912PG914SP916AR918PL920ML922KG924SF926WI928RR930DV932LE934DE936EY938CK940AL942GK944AL946AD948VN9504151-9406AI744DR746FT748GG750AA752DK754KK756FD758DS760PL762PG764SP766AR768PL770ML772KG774SF776WI778RR780DV782LE784DE786EY788CK790AL792GK794AL796AD798VN8004601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 26,8406,8606,8806,9006,9206,9406,9606,9807,000
    AATGGTTTATATCCTCTGGGTGGAAAATCCGCCATCGGATACGGACAAGTTAAGAGCCTGGGTATCAAGGGCGACGATAAGCGTATCAGCCGTTTAATGAATCCGGCCTTCGACGAAACCGACGTGGCGGTTCCAGAGAAGCCTAAAACCGATGCCGAGGTCCGTATCGAAGCTGTTACCAAATATAGGAGACCCACCTTTTAGGCGGTAGCCTATGCCTGTTCAATTCTCGGACCCATAGTTCCCGCTGCTATTCGCATAGTCGGCAAATTACTTAGGCCGGAAGCTGCTTTGGCTGCACCGCCAAGGTCTCTTCGGATTTTGGCTACGGCTCCAGGCATAGCTTCGACBmgBING802LY804PL806GG808KS810AI812GY814GQ816VK818SL820GI822KG824DD826KR828IS830RL832MN834PA836FD838ET840DV842AV844PE846KP848KT850DA852EV854RI856EA8584601-9406NG952LY954PL956GG958KS960AI962GY964GQ966VK968SL970GI972KG974DD976KR978IS980RL982MN984PA986FD988ET990DV992AV994PE996KP998KT1000DA1002EV1004RI1006EA10084151-9406NG802LY804PL806GG808KS810AI812GY814GQ816VK818SL820GI822KG824DD826KR828IS830RL832MN834PA836FD838ET840DV842AV844PE846KP848KT850DA852EV854RI856EA8584601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,0207,0407,0607,0807,1007,1207,1407,160
    AGAAGGTATATTATCCCCACTACTTTGTGGAACCGCACAAGAAGGTAGAGCGCGAGGAGAAGCCGTGCGGTCATCAAAAGTTTCATGAGGGACGCCTTACCGGTAAGATTCGTTGTAAACTTATTACGAAAACGCCTCTCATTGTGCCGGACACCAGCAATGATGACTTCTTTCGTCTTCCATATAATAGGGGTGATGAAACACCTTGGCGTGTTCTTCCATCTCGCGCTCCTCTTCGGCACGCCAGTAGTTTTCAAAGTACTCCCTGCGGAATGGCCATTCTAAGCAACATTTGAATAATGCTTTTGCGGAGAGTAACACGGCCTGTGGTCGTTACTACTGAAGAAAGCAgeIEK860VY862YP864HY866FV868EP870HK872KV874ER876EE878KP880CG882HQ884KF886HE888GR890LT892GK894IR896CK898LI900TK902TP904LI906VP908DT910SN912DD914FF916R4601-9406EK1010VY1012YP1014HY1016FV1018EP1020HK1022KV1024ER1026EE1028KP1030CG1032HQ1034KF1036HE1038GR1040LT1042GK1044IR1046CK1048LI1050TK1052TP1054LI1056VP1058DT1060SN1062DD1064FF1066R4151-9406EK860VY862YP864HY866FV868EP870HK872KV874ER876EE878KP880CG882HQ884KF886HE888GR890LT892GK894IR896CK898LI900TK902TP904LI906VP908DT910SN912DD914FF916R4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,1807,2007,2207,2407,2607,2807,3007,3207,340
    CCCTGCGGACAAAGAGGCCCGCAAGGAGAAGGACGAGTATCACAAATCCTATGCCTTCTTTCGCCTGCATAAGCAAATCATGATCCCCGGTAGTGAACTCCGCGGGATGGTGTCCTCTGTTTACGAAACGGTTACAAATTCATGTTTCCGTATCTTTGACGAGACTAAGCGTCTTGGGACGCCTGTTTCTCCGGGCGTTCCTCTTCCTGCTCATAGTGTTTAGGATACGGAAGAAAGCGGACGTATTCGTTTAGTACTAGGGGCCATCACTTGAGGCGCCCTACCACAGGAGACAAATGCTTTGCCAATGTTTAAGTACAAAGGCATAGAAACTGCTCTGATTCGCAGAAAhdIP918AD920KE922AR924KE926KD928EY930HK932SY934AF936FR938LH940KQ942IM944IP946GS948EL950RG952MV954SS956VY958ET960VT962NS964CF966RI968FD970ET972KR974L4601-9406P1068AD1070KE1072AR1074KE1076KD1078EY1080HK1082SY1084AF1086FR1088LH1090KQ1092IM1094IP1096GS1098EL1100RG1102MV1104SS1106VY1108ET1110VT1112NS1114CF1116RI1118FD1120ET1122KR1124L4151-9406P918AD920KE922AR924KE926KD928EY930HK932SY934AF936FR938LH940KQ942IM944IP946GS948EL950RG952MV954SS956VY958ET960VT962NS964CF966RI968FD970ET972KR974L4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,3607,3807,4007,4207,4407,4607,4807,5007,520
    AGTTGGCGCATGGATGCCGATCACCAAAACGTTTTACAAGATTTCTTACCGGGCCGTGTTACGGCCGACGGCAAGCACATTCAAAAGTTTTCCGAGACTGCACGTGTACCGTTTTATgacAAAACGCAAAAGCACTTTGACATTCTCGACGAGCAGGAAATTGCCGGTGAAAAGCTCAACCGCGTACCTACGGCTAGTGGTTTTGCAAAATGTTCTAAAGAATGGCCCGGCACAATGCCGGCTGCCGTTCGTGTAAGTTTTCAAAAGGCTCTGACGTGCACATGGCAAAATActgTTTTGCGTTTTCGTGAAACTGTAAGAGCTGCTCGTCCTTTAACGGCCACTTTTCGPmlIS976WR978MD980AD982HQ984NV986LQ988DF990LP992GR994VT996AD998GK1000HI1002QK1004FS1006ET1008AR1010VP1012FY1014DK1016TQ1018KH1020FD1022IL1024DE1026QE1028IA1030GE1032K4601-9406S1126WR1128MD1130AD1132HQ1134NV1136LQ1138DF1140LP1142GR1144VT1146AD1148GK1150HI1152QK1154FS1156ET1158AR1160VP1162FY1164DK1166TQ1168KH1170FD1172IL1174DE1176QE1178IA1180GE1182K4151-9406S976WR978MD980AD982HQ984NV986LQ988DF990LP992GR994VT996AD998GK1000HI1002QK1004FS1006ET1008AR1010VP1012FY1014DK1016TQ1018KH1020FD1022IL1024DE1026QE1028IA1030GE1032K4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,5407,5607,5807,6007,6207,6407,6607,6807,700
    CGGTCCGCATGTGGGTGAAGCGTTTCATTAAGCGCCTGAGCCTGGTGGACCCTGCAAAGCACCCACAAAAGAAGCAAGACAATAAGTGGAAACGCCGCAAGGAAGGTATTGCGACGTTCATTGAGCAGAAGAATGGGTCTTACTATTTTAACGTGGTCACCAACAATGGCTGCACGCCAGGCGTACACCCACTTCGCAAAGTAATTCGCGGACTCGGACCACCTGGGACGTTTCGTGGGTGTTTTCTTCGTTCTGTTATTCACCTTTGCGGCGTTCCTTCCATAACGCTGCAAGTAACTCGTCTTCTTACCCAGAATGATAAAATTGCACCAGTGGTTGTTACCGACGTGRsrIIP1034VR1036MW1038VK1040RF1042IK1044RL1046SL1048VD1050PA1052KH1054PQ1056KK1058QD1060NK1062WK1064RR1066KE1068GI1070AT1072FI1074EQ1076KN1078GS1080YY1082FN1084VV1086TN1088NG1090CT10924601-9406P1184VR1186MW1188VK1190RF1192IK1194RL1196SL1198VD1200PA1202KH1204PQ1206KK1208QD1210NK1212WK1214RR1216KE1218GI1220AT1222FI1224EQ1226KN1228GS1230YY1232FN1234VV1236TN1238NG1240CT12424151-9406P1034VR1036MW1038VK1040RF1042IK1044RL1046SL1048VD1050PA1052KH1054PQ1056KK1058QD1060NK1062WK1064RR1066KE1068GI1070AT1072FI1074EQ1076KN1078GS1080YY1082FN1084VV1086TN1088NG1090CT10924601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,7207,7407,7607,7807,8007,8207,8407,860
    CAGCTTTCACTTGTGGCACAAGCCAGACAACTTCGACCAAGAGAAGTTAGAGGGCATCCAAAACGGGGAGAAGTTAgatTGCTGGGTTCGTGATAGCCGTTACCAAAAGGCATTTCAAGAAATCCCGGAGAACGATCCGGACGGATGGGAGTGTAAGGAAGGTTATCTGCATGTGGTCGAAAGTGAACACCGTGTTCGGTCTGTTGAAGCTGGTTCTCTTCAATCTCCCGTAGGTTTTGCCCCTCTTCAATctaACGACCCAAGCACTATCGGCAATGGTTTTCCGTAAAGTTCTTTAGGGCCTCTTGCTAGGCCTGCCTACCCTCACATTCCTTCCAATAGACGTACACAjuISF1094HL1096WH1098KP1100DN1102FD1104QE1106KL1108EG1110IQ1112NG1114EK1116LD1118CW1120VR1122DS1124RY1126QK1128AF1130QE1132IP1134EN1136DP1138DG1140WE1142CK1144EG1146YL1148HV11504601-9406SF1244HL1246WH1248KP1250DN1252FD1254QE1256KL1258EG1260IQ1262NG1264EK1266LD1268CW1270VR1272DS1274RY1276QK1278AF1280QE1282IP1284EN1286DP1288DG1290WE1292CK1294EG1296YL1298HV13004151-9406SF1094HL1096WH1098KP1100DN1102FD1104QE1106KL1108EG1110IQ1112NG1114EK1116LD1118CW1120VR1122DS1124RY1126QK1128AF1130QE1132IP1134EN1136DP1138DG1140WE1142CK1144EG1146YL1148HV11504601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,8807,9007,9207,9407,9607,9808,0008,0208,040
    GTGGGCCCTTCGAAGGTTGAATTTAGCGACAAGAAGGGCGATGTCATTAATAACTTCCAAGGGACTCTCCCATCAGTGCCCAATGATTGGAAAACGATTCGTACAAACGACTTTAAGAACCGCAAACGTAAGAACGAGCCCGTTTTCTGTTGCGAGGATGACAAGGGGAACTACTCACCCGGGAAGCTTCCAACTTAAATCGCTGTTCTTCCCGCTACAGTAATTATTGAAGGTTCCCTGAGAGGGTAGTCACGGGTTACTAACCTTTTGCTAAGCATGTTTGCTGAAATTCTTGGCGTTTGCATTCTTGCTCGGGCAAAAGACAACGCTCCTACTGTTCCCCTTGATGAApaIPspOMIVG1152PS1154KV1156EF1158SD1160KK1162GD1164VI1166NN1168FQ1170GT1172LP1174SV1176PN1178DW1180KT1182IR1184TN1186DF1188KN1190RK1192RK1194NE1196PV1198FC1200CE1202DD1204KG1206NY12084601-9406VG1302PS1304KV1306EF1308SD1310KK1312GD1314VI1316NN1318FQ1320GT1322LP1324SV1326PN1328DW1330KT1332IR1334TN1336DF1338KN1340RK1342RK1344NE1346PV1348FC1350CE1352DD1354KG1356NY13584151-9406VG1152PS1154KV1156EF1158SD1160KK1162GD1164VI1166NN1168FQ1170GT1172LP1174SV1176PN1178DW1180KT1182IR1184TN1186DF1188KN1190RK1192RK1194NE1196PV1198FC1200CE1202DD1204KG1206NY12084601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 2Twist piece 38,0608,0808,1008,1208,1408,1608,1808,2008,220
    ACACGATGGCCAAATATTGTGAAACGTTCTTCTTCGACCTGAAAGAGAACGAGGAGTATGAGATCCCCGAGAAGGCACGTATCAAGTACAAGGAGTTATTACGCGTGTACAACAACAATCCGCAAGCGGTTCCCGAGAGCGTGTTCCAGAGTCGCGTAGCGCGCGAGAACGTAGATGTGCTACCGGTTTATAACACTTTGCAAGAAGAAGCTGGACTTTCTCTTGCTCCTCATACTCTAGGGGCTCTTCCGTGCATAGTTCATGTTCCTCAATAATGCGCACATGTTGTTGTTAGGCGTTCGCCAAGGGCTCTCGCACAAGGTCTCAGCGCATCGCGCGCTCTTGCATCTMluISspIBsrGIYT1210MA1212KY1214CE1216TF1218FF1220DL1222KE1224NE1226EY1228EI1230PE1232KA1234RI1236KY1238KE1240LL1242RV1244YN1246NN1248PQ1250AV1252PE1254SV1256FQ1258SR1260VA1262RE1264NV1266E4601-9406YT1360MA1362KY1364CE1366TF1368FF1370DL1372KE1374NE1376EY1378EI1380PE1382KA1384RI1386KY1388KE1390LL1392RV1394YN1396NN1398PQ1400AV1402PE1404SV1406FQ1408SR1410VA1412RE1414NV1416E4151-9406YT1210MA1212KY1214CE1216TF1218FF1220DL1222KE1224NE1226EY1228EI1230PE1232KA1234RI1236KY1238KE1240LL1242RV1244YN1246NN1248PQ1250AV1252PE1254SV1256FQ1258SR1260VA1262RE1264NV1266E4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,2408,2608,2808,3008,3208,3408,3608,3808,400
    AAAGCTGAAGTCTGGGGATTTGGTGTATTTCAAGCACAACGAGAAATATGTTGAGGACATCGTACCGGTTCGTATTTCACGTACCGTAGACGACCGCATGATCGGAAAGCGTATGTCGGCTGATTTGCGTCCATGTCACGGTGACTGGGTAGAGGACGGGGACCTCTCTGCGTTGTTTCGACTTCAGACCCCTAAACCACATAAAGTTCGTGTTGCTCTTTATACAACTCCTGTAGCATGGCCAAGCATAAAGTGCATGGCATCTGCTGGCGTACTAGCCTTTCGCATACAGCCGACTAAACGCAGGTACAGTGCCACTGACCCATCTCCTGCCCCTGGAGAGACGCAACAgeIK1268LK1270SG1272DL1274VY1276FK1278HN1280EK1282YV1284ED1286IV1288PV1290RI1292SR1294TV1296DD1298RM1300IG1302KR1304MS1306AD1308LR1310PC1312HG1314DW1316VE1318DG1320DL1322SA1324L4601-9406K1418LK1420SG1422DL1424VY1426FK1428HN1430EK1432YV1434ED1436IV1438PV1440RI1442SR1444TV1446DD1448RM1450IG1452KR1454MS1456AD1458LR1460PC1462HG1464DW1466VE1468DG1470DL1472SA1474L4151-9406K1268LK1270SG1272DL1274VY1276FK1278HN1280EK1282YV1284ED1286IV1288PV1290RI1292SR1294TV1296DD1298RM1300IG1302KR1304MS1306AD1308LR1310PC1312HG1314DW1316VE1318DG1320DL1322SA1324L4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,4208,4408,4608,4808,5008,5208,5408,560
    AATGCATATCCAGAGAAGCGCCTTTTATTGCGTCACCCTAAGGGCCTTTGCCCGGCATGTCGCCTTTTCGGAACGGGTAGCTACAAGGGCCGCGTTCGTTTTGGCTTCGCGAGCCTGGAGAACgacCCGGAGTGGTTGATTCCTGGAAAGAATCCCGGTGATCCATTCCACGGCGTTACGTATAGGTCTCTTCGCGGAAAATAACGCAGTGGGATTCCCGGAAACGGGCCGTACAGCGGAAAAGCCTTGCCCATCGATGTTCCCGGCGCAAGCAAAACCGAAGCGCTCGGACCTCTTGctgGGCCTCACCAACTAAGGACCTTTCTTAGGGCCACTAGGTAAGGTGCCGCNruINsiIBsu36IN1326AY1328PE1330KR1332LL1334LR1336HP1338KG1340LC1342PA1344CR1346LF1348GT1350GS1352YK1354GR1356VR1358FG1360FA1362SL1364EN1366DP1368EW1370LI1372PG1374KN1376PG1378DP1380FH1382G4601-9406N1476AY1478PE1480KR1482LL1484LR1486HP1488KG1490LC1492PA1494CR1496LF1498GT1500GS1502YK1504GR1506VR1508FG1510FA1512SL1514EN1516DP1518EW1520LI1522PG1524KN1526PG1528DP1530FH1532G4151-9406N1326AY1328PE1330KR1332LL1334LR1336HP1338KG1340LC1342PA1344CR1346LF1348GT1350GS1352YK1354GR1356VR1358FG1360FA1362SL1364EN1366DP1368EW1370LI1372PG1374KN1376PG1378DP1380FH1382G4601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,5808,6008,6208,6408,6608,6808,7008,7208,740
    GGCCAGTGATGCTGAGCTTGCTGGAGCGCCCGCGTCCCACCTGGAGCATTCCTGGCAGTGATAATAAGTTCAAAGTTCCAGGCCGTAAGTTCTACGTTCACCATCATGCTTGGAAAACGATTAAGGACGGTAACCATCCGACCACCGGCAAAGCCATTGAACAATCGCCAAATAACCGGTCACTACGACTCGAACGACCTCGCGGGCGCAGGGTGGACCTCGTAAGGACCGTCACTATTATTCAAGTTTCAAGGTCCGGCATTCAAGATGCAAGTGGTAGTACGAACCTTTTGCTAATTCCTGCCATTGGTAGGCTGGTGGCCGTTTCGGTAACTTGTTAGCGGTTTATTG1384PV1386ML1388SL1390LE1392RP1394RP1396TW1398SI1400PG1402SD1404NK1406FK1408VP1410GR1412KF1414YV1416HH1418HA1420WK1422TI1424KD1426GN1428HP1430TT1432GK1434AI1436EQ1438SP1440NN14424601-9406G1534PV1536ML1538SL1540LE1542RP1544RP1546TW1548SI1550PG1552SD1554NK1556FK1558VP1560GR1562KF1564YV1566HH1568HA1570WK1572TI1574KD1576GN1578HP1580TT1582GK1584AI1586EQ1588SP1590NN15924151-9406G1384PV1386ML1388SL1390LE1392RP1394RP1396TW1398SI1400PG1402SD1404NK1406FK1408VP1410GR1412KF1414YV1416HH1418HA1420WK1422TI1424KD1426GN1428HP1430TT1432GK1434AI1436EQ1438SP1440NN14424601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,7608,7808,8008,8208,8408,8608,8808,9008,920
    CCGCACGGTTGAAGCGCTCGCCGGTGGCAACAGCTTCAGCTTCGAAATTGCGTTCGAAAATTTGAAGGAGTGGGAGTTGGGCCTGCTGATCCATAGCCTGCAGCTTGAAAAGGGCTTGGCCCATAAGCTCGGTATGGCTAAGAGCATGGGCTTTGGCTCAGTAGAGATCGACGTTGGCGTGCCAACTTCGCGAGCGGCCACCGTTGTCGAAGTCGAAGCTTTAACGCAAGCTTTTAAACTTCCTCACCCTCAACCCGGACGACTAGGTATCGGACGTCGAACTTTTCCCGAACCGGGTATTCGAGCCATACCGATTCTCGTACCCGAAACCGAGTCATCTCTAGCTGCAAPstIRT1444VE1446AL1448AG1450GN1452SF1454SF1456EI1458AF1460EN1462LK1464EW1466EL1468GL1470LI1472HS1474LQ1476LE1478KG1480LA1482HK1484LG1486MA1488KS1490MG1492FG1494SV1496EI1498DV15004601-9406RT1594VE1596AL1598AG1600GN1602SF1604SF1606EI1608AF1610EN1612LK1614EW1616EL1618GL1620LI1622HS1624LQ1626LE1628KG1630LA1632HK1634LG1636MA1638KS1640MG1642FG1644SV1646EI1648DV16504151-9406RT1444VE1446AL1448AG1450GN1452SF1454SF1456EI1458AF1460EN1462LK1464EW1466EL1468GL1470LI1472HS1474LQ1476LE1478KG1480LA1482HK1484LG1486MA1488KS1490MG1492FG1494SV1496EI1498DV15004601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,9408,9608,9809,0009,0209,0409,0609,0809,100
    GAGAGTGTCCGCCTGCGTAAAGATTGGAAGCAGTGGCGCAATGGTAATTCAGAGATCCCAAATTGGTTGGGCAAGGGGTTTGCAAAGCTGAAGGAGTGGTTCCGCGACGAGCTGGACTTCATCGAGAATTTGAAGAAATTACTGTGGTTCCCCGAAGGTGATCAAGCTCCGCGCGCTCTCACAGGCGGACGCATTTCTAACCTTCGTCACCGCGTTACCATTAAGTCTCTAGGGTTTAACCAACCCGTTCCCCAAACGTTTCGACTTCCTCACCAAGGCGCTGCTCGACCTGAAGTAGCTCTTAAACTTCTTTAATGACACCAAGGGGCTTCCACTAGTTCGAGGCGCGCES1502VR1504LR1506KD1508WK1510QW1512RN1514GN1516SE1518IP1520NW1522LG1524KG1526FA1528KL1530KE1532WF1534RD1536EL1538DF1540IE1542NL1544KK1546LL1548WF1550PE1552GD1554QA1556PR15584601-9406ES1652VR1654LR1656KD1658WK1660QW1662RN1664GN1666SE1668IP1670NW1672LG1674KG1676FA1678KL1680KE1682WF1684RD1686EL1688DF1690IE1692NL1694KK1696LL1698WF1700PE1702GD1704QA1706PR17084151-9406ES1502VR1504LR1506KD1508WK1510QW1512RN1514GN1516SE1518IP1520NW1522LG1524KG1526FA1528KL1530KE1532WF1534RD1536EL1538DF1540IE1542NL1544KK1546LL1548WF1550PE1552GD1554QA1556PR15584601-9403Translation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 39,1209,1409,1609,1809,2009,2209,2409,260
    TATGTTACCCCATGCTTCGCAAGAAGGATGACCCTAATGGAAACTCAGGTTATGAGGAGTTGAAAGACGGCGAATTTAAGAAGGAAGATCGTCAGAAGAAGCTTACAACACCATGGACGCCGTGGGCTTGAGCGGCCGCACTCGAGGCCCGAAAGGAAGCTGAGTTGGCTGCTGCATACAATGGGGTACGAAGCGTTCTTCCTACTGGGATTACCTTTGAGTCCAATACTCCTCAACTTTCTGCCGCTTAAATTCTTCCTTCTAGCAGTCTTCTTCGAATGTTGTGGTACCTGCGGCACCCGAACTCGCCGGCGTGAGCTCCGGGCTTTCCTTCGACTCAACCGACGACGNcoINotITliIXhoIPspXIPaeR7IVC1560YP1562ML1564RK1566KD1568DP1570NG1572NS1574GY1576EE1578LK1580DG1582EF1584KK1586ED1588RQ1590KK1592LT1594TP1596WT1598PW1600A*16024601-9406VC1710YP1712ML1714RK1716KD1718DP1720NG1722NS1724GY1726EE1728LK1730DG1732EF1734KK1736ED1738RQ1740KK1742LT1744TP1746WT1748PW1750A*17524151-9406VC1560YP1562ML1564RK1566KD1568DP1570NG1572NS1574GY1576EE1578LK1580DG1582EF1584KK1586ED1588RQ1590KK1592LT1594TP1596WT1598PW1600A4601-9403Translation 4151-9406Translation 4601-9406Translation 4601-9403sourceTwist piece 3T7_terminator9,2809,3009,3209,3409,3609,3809,4009,4209,440
    CACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTATAGGGCGTTCTCCGGGCCGTCATGGCCGTATTGGTTCGGATACGGATGTCGTAGGTCCCACTGCCACGGCTCCTACTGCTACTCGCEcoRVT7_terminatorT7_Ter...rimer9,4609,4809,5009,5209,5409,5609,5809,6009,620
    CATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGAGTAACAATCTAAAGTATGTGCCACGGACTGACGCAATCGTTAAATTGACACTATTTGATGGCGTAATTTCGAATAGCTACTATTCGACAGTTTGTACTCTTAAGAACTTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTClaIBspDIEcoRIFP03029,6409,6609,6809,7009,7209,7409,7609,7809,800
    CGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAASspIZraIAatIIAmpicillinAmpR_promoterFP03029,8209,8409,8609,8809,9009,9209,9409,960
    TGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAAmpicillin9,98010,00010,02010,04010,06010,08010,10010,12010,140
    CTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACAGACGATACACCGCGCCATAATAGGGCACAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTScaIAmpicillin10,16010,18010,20010,22010,24010,26010,28010,30010,320
    CTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACGTCGTTACCGTTGTTGCAACGCPstIAmpicillin10,34010,36010,38010,40010,42010,44010,46010,48010,500
    CAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACBsaIAmpicillin10,52010,54010,56010,58010,60010,62010,64010,660
    GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAAAhdIAmpicillin10,68010,70010,72010,74010,76010,78010,80010,82010,840
    AATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAG10,86010,880
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10889 bp800085009000950010000105005001000150020002500Translation 4151-9406Translation 4601-9406Translation 4601-9403sourcelacITwist piece 3ROPROP4601-94064601-94034151-9406oriPciIBspQI,SapI,BstZ17I,PflFI,Tth111IBsmBIEsp3IpGEX_3_primerFspAIBstAPINruItet (576 - 1190)BsmBI,Esp3ISspIMluI,BsrGIAgeINsiI,Bsu36INruIPstIT7_Terminal_primer,NcoI,NotI,PaeR7I,PspXI,TliI,XhoIT7_terminator,EcoRVFP0302,BspDI,ClaI,EcoRI,ZraI,AatIIAmpR_promoter,SspIAmpicillin,ScaIPstI,BsaI,AhdI

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    10889