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PGK-mCherry
  • Sequence Map
    Editing disabled on read-only sequence.
    ttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccacaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtgori20406080100120140160
    cacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaaccori180200220240260280300320340
    agcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaori360380400420440460480500520
    tcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaaPciIAflIIIori540560580600620640660680700
    gagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttSapIBspQIA1P2N3T4Q5T6A7S8P9R10A11L12A13D14S15L16M17Q18L19A20R21Q22V23S24R25L26E27S28G29Q30*31lacI (fragment)720740760780800820840860
    ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagctctagctagaggtcgacggtatacagacatgataagatacattaattacactcaatcgagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgagatcgatctccagctgccatatgtctgtactattctatgtaaBstZ17Ilac promoterCAP binding sitelac operatorSV...l8809009209409609801,0001,0201,040
    gatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttggggtgggcgaagaactccagcatgagatccccgcgctggaggatcatccagccggcgtcccggctactcaaacctgtttggtgttgatcttacgtcactttttttacgaaataaacactttaaacactacgataacgaaataaacattggtaatattcgacgttatttgttcaaccccacccgcttcttgaggtcgtactctaggggcgcgacctcctagtaggtcggccgcagggccPfoIPsiIL42N41P40H39A38F37F36E35L34M33L32D31G30R29Q28L27I26M25W24G23A22D21R20ble (fragment)SV40 poly(A) signal1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aaaacgattccgaagcccaacctttcatagaaggcggcggtggaatcgaaatctcgtagtacgtgctattcctttgccctcggacgagtgctggggcgtcggtttccactatcggcgagtacttctacacagccatcggtccagacggccgcgcttctgcgggcgatttgtgtacttttgctaaggcttcgggttggaaagtatcttccgccgccaccttagctttagagcatcatgcacgataaggaaacgggagcctgctcacgaccccgcagccaaaggtgatagccgctcatgaagatgtgtcggtagccaggtctgccggcgcgaagacgcccgctaaacacatgScaIF19V18I17G16F15G14L13R12E11Y10F9A8A7T6S5D4F3D2R1ble (fragment)*E340KA338RP336RT334SP332RR330NG328SD326AL324VE322VC320GD318TW316VA314AS312RR310AI308QT306RHygR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gcccgacagtcccggctccggatcggacgattgcgtcgcatcgaccctgcgcccaagctgcatcatcgaaattgccgtcaaccaagctctgatagagttggtcaagaccaatgcggagcatatacgcccggagccgcggcgatcctgcaagctccggatgcctccgctcgaagtacgggctgtcagggccgaggcctagcctgctaacgcagcgtagctgggacgcgggttcgacgtagtagctttaacggcagttggttcgagactatctcaaccagttctggttacgcctcgtatatgcgggcctcggcgccgctaggacgttcgaggcctacggaggcgagcttcatSacIIG304VT302GA300GS298RV296IA294DC292RG290QA288WA286AD284DF282NG280DV278LS276QY274LQ272DL270GI268RL266MY264AR262LR260PS258GA256LE254PH252RR250EF248YHygR1,4201,4401,4601,4801,5001,5201,5401,560
    gcgcgtctgctgctccatacaagccaaccacggcctccagaagaagatgttggcgacctcgtattgggaatccccgaacatcgcctcgctccagtcaatgaccgctgttatgcggccattgtccgtcaggacattgttggagccgaaatccgcgtgcacgaggtgccggacttcgcgcgcagacgacgaggtatgttcggttggtgccggaggtcttcttctacaaccgctggagcataacccttaggggcttgtagcggagcgaggtcagttactggcgacaatacgccggtaacaggcagtcctgtaacaacctcggctttaggcgcacgtgctccacggcctgaagcAjuIBstAPIR246TQ244QE242MC240AL238WP236RW234FF232IN230AV228EY226QS224DG222FM220AE218SW216DI214VA212TI210RG208ND206TL204VN202NS200GF198DA196HV194LH192RV190EHygR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gggcagtcctcggcccaaagcatcagctcatcgagagcctgcgcgacggacgcactgacggtgtcgtccatcacagtttgccagtgatacacatggggatcagcaatcgcgcatatgaaatcacgccatgtagtgtattgaccgattccttgcggtccgaatgggccgaacccgccccgtcaggagccgggtttcgtagtcgagtagctctcggacgcgctgcctgcgtgactgccacagcaggtagtgtcaaacggtcactatgtgtacccctagtcgttagcgcgtatactttagtgcggtacatcacataactggctaaggaacgccaggcttacccggcttgggcgNdeIPflFIRsrIITth111IP188CD186EA184WL182ML180ED178LA176QA174VS172AS170VT168DD166MV164TQ162WH160YV158HP156DA154IA152CI150FD148RW146TT144YQ142GI140GQ138PG136FP134GF132GS130HygR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tcgtctggctaagatcggccgcagcgatcgcatccatggcctccgcgaccggctgcagaacagcgggcagttcggtttcaggcaggtcttgcaacgtgacaccctgtgcacggcgggagatgcaataggtcaggctctcgctgaattccccaatgtcaagcacttccggaatcggagcagaccgattctagccggcgtcgctagcgtaggtaccggaggcgctggccgacgtcttgtcgcccgtcaagccaaagtccgtccagaacgttgcactgtgggacacgtgccgccctctacgttatccagtccgagagcgacttaaggggttacagttcgtgaaggccttagccPvuIAsiSIEcoRIBstAPITQ128SL126DA124AA122IA120DM118AE116AV114PQ112LV110AP108LE106TE104PL102DQ100LT98VG96QA94RR92SI90CY88TL86SE84SF82EG80ID78LV76EP74IP72HygR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gagcgcggccgatgcaaagtgccgataaacataacgatctttgtagaaaccatcggcgcagctatttacccgcaggacatatccacgccctcctacatcgaagctgaaagcacgagattcttcgccctccgagagctgcatcaggtcggagacgctgtcgaacttttcgatcagactcgcgccggctacgtttcacggctatttgtattgctagaaacatctttggtagccgcgtcgataaatgggcgtcctgtataggtgcgggaggatgtagcttcgactttcgtgctctaagaagcgggaggctctcgacgtagtccagcctctgcgacagcttgaaaagctagtctPflFITth111ILA70AS68AF66HR64YV62YR60DK58YF56GD54AC52SN50VR48LV46YG44RG42GV40DF38SF36AR34SE32EG30ES28LQ26ML24DS22VS20DF18KE16IL14HygR2,1202,1402,1602,1802,2002,2202,2402,260
    aacttctcgacagacgtcgcggtgagttcaggctttttggccaaggaagttcctatactttctagagaataggaacttcggaataggaacttctaggtacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagccttgaagagctgtctgcagcgccactcaagtccgaaaaaccggttccttcaaggatatgaaagatctcttatccttgaagccttatccttgaagatccatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcggBarIXbaIPshAIFK12EV10ST8AT6LE4PK2KHygRf1 ori (fragment)FRT2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtggggataccccctagagccgggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcacccctatgggggatctcggf1 ori (fragment)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ccagctggttctttccgcctcagaagccatagagcccaccgcatccccagaacatcgatagatctccatagagcccaccgcatccccagcatgcctgctattgtcttcccaatcctcccccttgctgtcctgccccaccccaccccccagaatagaatgacacctactcagacaaggtcgaccaagaaaggcggagtcttcggtatctcgggtggcgtaggggtcttgtagctatctagaggtatctcgggtggcgtaggggtcgtacggacgataacagaagggttaggagggggaacgacaggacggggtggggtggggggtcttatcttactgtggatgagtctgttClaIBspDIbGH poly(A) signalbGH poly(...ragment)SV40 pro...agment)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgcgatgcaatttcctcattttattaggaaaggacagtgggagtggcaccttccagggtcaaggaaggcacgggggaggggcaaacaacagatggctggcaactagaaggcacagtcgaggctgatcagcgggtttaaactcaatgatgattatgatgatggtcgacggcgctatacgctacgttaaaggagtaaaataatcctttcctgtcaccctcaccgtggaaggtcccagttccttccgtgccccctccccgtttgttgtctaccgaccgttgatcttccgtgtcagctccgactagtcgcccaaatttgagttactactaatactactaccagctgccgcgataBclIPmeIbGH poly(A) signal2,8202,8402,8602,8802,9002,9202,9402,960
    tcagatcctcttctgagatgagtttttgttcgggccctcctcgagcttaagacttgtacagctcgtccatgccgccggtggagtggcggccctcggcgcgttcgtactgttccacgatggtgtagtcctcgttgtgggaggtgatgtccaacttgatgttgacgttgtaggcgccagtctaggagaagactctactcaaaaacaagcccgggaggagctcgaattctgaacatgtcgagcaggtacggcggccacctcaccgccgggagccgcgcaagcatgacaaggtgctaccacatcaggagcaacaccctccactacaggttgaactacaactgcaacatccgcggAleIApaITliIXcmIXhoIAflIIBsrGIPspXISgrAIPaeR7IPspOMIK236YL234ED232MG230GT228SH226RG224EA222RE220YQ218EV216IT214YD212EN210HS208TI206DL204KI202NV200NY198AG196mCherry2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gggcagctgcacgggcttcttggccttgtaggtggtcttgacctcagcgtcgtagtggccgccgtccttcagcttcagcctctgcttgatctcgcccttcagggcgccgtcctcggggtacatccgctcggaggaggcctcccagcccatggtcttcttctgcattacggggccgcccgtcgacgtgcccgaagaaccggaacatccaccagaactggagtcgcagcatcaccggcggcaggaagtcgaagtcggagacgaactagagcgggaagtcccgcggcaggagccccatgtaggcgagcctcctccggagggtcgggtaccagaagaagacgtaatgccccggcBplIBplIBbvCIPflMIPL194QV192PK190KA188KY186TT184KV182EA180DY178HG176GD174KL172KL170RQ168KI166EG164KL162AG160DE158PY156MR154ES152SA150EW148GM146TK144KQ142MV140PG138mCherry3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tcggaggggaagttggtgccgcgcagcttcaccttgtagatgaactcgccgtcctgcagggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcaagtagtcgggaatgtcggcggggtgctagcctccccttcaaccacggcgcgtcgaagtggaacatctacttgagcggcaggacgtccctcctcaggacccagtgccagtggtgcggcggcaggagcttcaagtagtgcgcgagggtgaacttcgggagccccttcctgtcgaagttcatcagcccttacagccgccccacgaAloISbfIBstEIIDS136PF134NT132GR130LK128VK126YI124FE122GD120QL118SS116DQ114TV112TV110VG108GD106EF104NM102VR100EW98KF96GE94PF92SL90KL88YD86PI84DA82PH80KmCherry3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tcacgtaggccttggagccgtacatgaactgaggggacaggatgtcccaggcgaagggcagggggccacccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgtgcacagtgcatccggaacctcggcatgtacttgactcccctgtcctacagggtccgcttcccgtcccccggtgggaaccagtggaagtcgaaccgccagacccacgggagcatccccgccgggagcgggagcgggagctagagcttgagcaccggcaagtgcctcgggaggtacacgtgBstEIIV78YA76KS74GY72MF70QP68SL66ID64WA62FP60LP58GG56KT54VK52LK50AT48QT46GE44YP42RG40EG38EG36EI34EF32EH30GN28VS26GE24MH22VmCherry3,5203,5403,5603,5803,6003,6203,6403,660
    cttgaagcgcatgaactccttgatgatggccatgttatcctcctcgcccttgctcaccatcctaggcttagcgctggtgtacttggtgatggccttagtaccctcggacacggcgtgcttggccaactccccaggcagcagcaggcgcacggccgtctggatctccctggaggtggaacttcgcgtacttgaggaactactaccggtacaataggaggagcgggaacgagtggtaggatccgaatcgcgaccacatgaaccactaccggaatcatgggagcctgtgccgcacgaaccggttgaggggtccgtcgtcgtccgcgtgccggcagacctagagggacctccacAfeIBlpIAvrIIP1R2KA124ST122YK120TI118AK116TG114ES112VA110HK108AL106EG104PL102LL100RV98AT96QI94ER92ST90H2BK20FR18MF16EK14II12AM10ND8EE6GK4SV2MmCherry3,6803,7003,7203,7403,7603,7803,8003,8203,840
    atggtcgagcgcttgttgtaatgcgccaggcgggaagcctcacctgcgatgcgctcgaaaatgtcgttcacaaacgaattcatgatgcccatggccttggacgaaatgccggtgtcagggtggacctgcttcagaaccttgtacacatagatggaatagctctccttgcggctgctaccagctcgcgaacaacattacgcggtccgcccttcggagtggacgctacgcgagcttttacagcaagtgtttgcttaagtactacgggtaccggaacctgctttacggccacagtcccacctggacgaagtcttggaacatgtgtatctaccttatcgagaggaacgccgacgAfeIBsrGIBstXIEcoRIPaqCIIT88SR86KN84YH82AL80RS78AE76GA74IR72EF70ID68NV66FS64NM62IG60MA58KS56SI54GT52DP50HV48QK46LV44KY42VY40IS38YS36EK34RS32RH2B3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcttgcgcttcttgccgcctttcttctgcgccttagtcaccgccttcttggagccctttttcggggcgggagcagacttcgctggctctgggtgcatggtggcgactgcaggtcgaaaggcccggagatgaggaagaggagaacagcgcggcagacgtgcgcttttgaagcgtgccgaacgcgaagaacggcggaaagaagacgcggaatcagtggcggaagaacctcgggaaaaagccccgccctcgtctgaagcgaccgagacccacgtaccaccgctgacgtccagctttccgggcctctactccttctcctcttgtcgcgccgtctgcacgcgaaaacttcgcacgH2M1K30RK28KG26GK24KQ22AK20TV18AK16KS14GK12KP10AP8AS6KA4PE2PH2BPGK promoter4,0404,0604,0804,1004,1204,1404,1604,1804,200
    agaatgccgggcctccggaggaccttcgggcgcccgccccgcccctgagcccgcccctgagcccgcccccggacccaccccttcccagcctctgagcccagaaagcgaaggagcaaagctgctattggccgctgccccaaaggcctacccgcttccattgctcagcggtgctgtctcttacggcccggaggcctcctggaagcccgcgggcggggcggggactcgggcggggactcgggcgggggcctgggtggggaagggtcggagactcgggtctttcgcttcctcgtttcgacgataaccggcgacggggtttccggatgggcgaaggtaacgagtcgccacgacagBlpIPpuMIPGK promoter4,2204,2404,2604,2804,3004,3204,3404,360
    catctgcacgagactaatgagacgtgctacttccatttgtcacgtcctgcacgacgcgagctgcggggcgggggggaacttcctgactaggggaggagtagaaggtggcgcgaaggggccaccaaagaacggagccggttggcgcctaccggtggatgtggaatgtgtgcgaggcgtagacgtgctctgattactctgcacgatgaaggtaaacagtgcaggacgtgctgcgctcgacgccccgcccccccttgaaggactgatcccctcctcatcttccaccgcgcttccccggtggtttcttgcctcggccaaccgcggatggccacctacaccttacacacgctccgAgeIPGK promoter4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cagaggccacttgtgtagcgccaagtgcccagcggggctgctaaagcgcatgctccagactgccttgggaaaagcgcctcccctacccggtagagcggccgcataacttcgtatagcatacattatacgaagttatgctagcctcgactgtgccttctagttgccagccatctgtgtctccggtgaacacatcgcggttcacgggtcgccccgacgatttcgcgtacgaggtctgacggaacccttttcgcggaggggatgggccatctcgccggcgtattgaagcatatcgtatgtaatatgcttcaatacgatcggagctgacacggaagatcaacggtcggtagacaBmtINheINotIPGK promoterloxPbGH poly(A) signal4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgabGH poly(A) signal4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggggaagatatagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggaccccttctatatccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctPasISmaISmaISrfIXmaIXmaIRsrIITspMITspMI4,9204,9404,9604,9805,0005,0205,0405,060
    tcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaagccataccaatgggccctaaaaaaggaatccagtcaattccggggctaaacctggctgccactgtttctttagggacttcgttcctgtgaggacaggaaaccctgcaagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgcccctttttcggtatggttacccgggattttttccttaggtcagttaaggccccgatttggaccgacggtgacaaagaaatccctgaagcaaggacactcctgtcctttgggacgtApaIBstXIEcoNIPspOMI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    acctggttgaccagagcaagggatataagaacccagtttgggaacactgctgtttaaatattaaacagggatgtcttgtcccccggaagggagaccagatgagcttctgatagactaattgacatcatttgagtcaattccagacatgataagatacattgatgagtttggacaatggaccaactggtctcgttccctatattcttgggtcaaacccttgtgacgacaaatttataatttgtccctacagaacagggggccttccctctggtctactcgaagactatctgattaactgtagtaaactcagttaaggtctgtactattctatgtaactactcaaacctgttBsaISspISexAIG-CSF SLDESV40 poly(A) signal5,2605,2805,3005,3205,3405,3605,3805,4005,420
    accacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaagcttaagtttaaacgctagagtccggaggctggatcggtcccggtgtcttctatggaggtcaaaacagcgtggatggtggtgttgatcttacgtcactttttttacgaaataaacactttaaacactacgataacgaaataaacattggtaatattcgacgttatttgttcaattcgaattcaaatttgcgatctcaggcctccgacctagccagggccacagaagatacctccagttttgtcgcacctaccPmeIPsiIAflIIHindIIISV40 poly(A) signal5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgtctccaggcgatctgacggttcactaaacgagctcgtcgacgatctctatcactgatagggagatctctatcactgatagggagagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgcagaggtccgctagactgccaagtgatttgctcgagcagctgctagagatagtgactatccctctagagatagtgactatccctctcgagacgaatatatctggagggtggcatgtgcggatggcgggtaaacgcagttaccccgcctcaacaatgctgtaaaacctttcagggSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoterTRE3GV promoter (fragment)PLtetO-...gment)PLtetO-...gment)5,6205,6405,6605,6805,7005,7205,7405,760
    gttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcaactaaaaccacggttttgtttgagggtaactgcagttaccccacctctgaacctttaggggcactcagtttggcgataggtgcgggtaactacatgacggttttggcgtagtggtaccattatcgctactgattatgcatctacatgacggttcatcctttcagggtattccaCspCISnaBICMV promoterCMV enhancer5,7805,8005,8205,8405,8605,8805,9005,9205,940
    catgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattgtacatgacccgtattacggtccgcccggtaaatggcagtaactgcagttatcccccgcatgaaccgtatactatgtgaactacatgacggttcacccgtcaaatggcatttatgaggtgggtaactgcagttacctttcagggataaccgcaatgatacccttgtatgcagtaaNdeICMV enhancer5,9605,9806,0006,0206,0406,0606,0806,1006,120
    attgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgcggaactccatatatgggctatgaactaatgaccccgtaattgattactattaataactagtcaataatcaatgtcaacgcgtatatctggcccgtacgaagcagctaactgcagttacccgcccccagcaacccgccagtcggtccgcccggtaaatggcattcaatacattgcgccttgaggtatatacccgatacttgattactggggcattaactaatgataattattgatcagttattagttacagttgcgcatatagaccgggcatgcttcgtcgMluISpeIBsiWIAflIIICMV enhancer6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gcaaaacgcctaaccctaagcagattcttcatgcaattgtcggtcaagccttgccttgttgtagcttaaattttgctcgcgcactactcagcgacctccaacacacaagcagggagcagatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatagggcgttttgcggattgggattcgtctaagaagtacgttaacagccagttcggaacggaacaacatcgaatttaaaacgagcgcgtgatgagtcgctggaggttgtgtgttcgtccctcgtctatgaccgaattgatacgccgtagtctcgtctaacatgactctcacgtggtatcccMfeIbom (fragment)6,3206,3406,3606,3806,4006,4206,4406,460
    gatcgggagatctcccgatccgtcgacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatctagccctctagagggctaggcagctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaSspISgrDIMS2IQ4HAmpRAmpR promoter6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaAclIXmnIF6RV8AL10IP12FF14AA16FC18LP20VF22AH24PE26TL28VK30VK32DA34ED36QL38GA40RV42GY44IE46LD48LN50SG52KI54LE56SF58RP60EE62RAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcBcgIScaIF64PM66MS68TF70KV72LL74CG76AV78LS80RI82DA84GQ86EQ88LG90RR92IH94YS96QN98DL100VE102YS104PV106TE108KH110LT112DG114MT116VR118EL120CS122AmpR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacPvuIAA124IT126MS128DN130TA132AN134LL136LT138TI140GG142PK144EL146TA148FL150HN152MG154DH156VT158RL160DR162WE164PE166LN168EA170IP172ND174ER176DT178TM180AmpR7,0207,0407,0607,0807,1007,1207,1407,160
    cctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacAclIFspIPV182AM184AT186TL188RK190LL192TG194EL196LT198LA200SR202QQ204LI206DW208ME210AD212KV214AG216PL218LR220SA222LP224AG226WF228IA230DK232SG234AG236ER238AmpR7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaBsaIGS240RG242II244AA246LG248PD250GK252PS254RI256VV258IY260TT262GS264QA266TM268DE270RN272RQ274IA276EI278GA280SL282IK284HW286*AmpR7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaag7,5407,5607,5807,6007,6207,6407,660
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    7666 bp550060006500700050010001500HygRoriAflIII,PciICAP binding site,lac promoter,lac operator,lacI (fragment),BspQI,SapISV40 poly(A) signal,BstZ17I,PsiIble (fragment),PfoIScaISacII,AjuIBstAPIPflFITth111I,NdeI,RsrIICMV promoter,CspCISnaBI,NdeICMV enhancerSpeI,AflIII,MluI,BsiWI,MfeIbom (fragment),SgrDIAmpR promoter,SspIAclI,XmnIAmpR,BcgI,ScaI,PvuI,AclI,FspIBsaI

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    7666