Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV-nEF-DIO-eNPAC 2.0-WPRE
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaaggatctgcgatcgctccgggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcctagacgctagcgaggcMluIPvuISbfIAsiSIL-ITREF1 promoter20406080100120140160
    gtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtcacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttgcccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcaMfeIEcoNIEF1 promoter180200220240260280300320340
    agtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgcctcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtcgacttcgaagctccccgagcgtagagaggaagtgcgcgggcggcgggatggactccggcggtaggtgcggccaactcagcgcaagacggcggagggcggacaccacggaggacttgacgcaggcggCspCIEcoNIBsu36IHindIIIEF1 promoter360380400420440460480500520
    gtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacagatccattcaaatttcgagtccagctctggcccggaaacaggccgcgagggaacctcggatggatctgagtcggccgagaggtgcgaaacggactgggacgaacgagttgagatgcagaaacaaagcaaaagacaagacgcggcaatgtctaggttcgacactggccgcggatKasINarISfoIPluTIEF1 promoter540560580600620640660680700
    cggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgccttacttgtacagctcgtccatgccgcctaggagatctcagctgaggccttattgaagcatatcctatgaaatatgcttcaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcggaatgaacatgtcgagcaggtacggAccIAscISalIXbaIBamHIBspEIBsrGIlox2722loxPEYFP720740760780800820840860
    gagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacgagtcccgcctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccccacaagacgaccatcaccagccgctcgacgtgcgacggcEYFP8809009209409609801,0001,0201,040
    tcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccctgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggagggacttcagctacgggaagtcgagctacgccaagtggtcccacagcgggaEYFP1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgaagcactgcaggccgtagccgaaggtggtcacgagggtgggccagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcttcgtgacgtccggcatcggcttccaccagtgctcccacccggtEYFP1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcacggcggcccccgtgcccgtcgaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctacccgtggtggggccacttgtcgaggagcgggaacgagtgccgccggEYFP1,4201,4401,4601,4801,5001,5201,5401,560
    gctggcacggctccggcctcggcttcgtcttcgacgagagtctcgacctcgatctccgttccgccgatgttcagtttggtggttttgcggatatctccgtgaatcaatatgtgctcgtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcgaccgtgccgaggccggagccgaagcagaagctgctctcagagctggagctagaggcaaggcggctacaagtcaaaccaccaaaacgcctatagaggcacttagttatacacgagcacctagtcctgcgcgtccatcacagggttgttgggggttgttaaaaatgagtagtctaBfuAIBspMIPshAIhChR2 (H134R)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    caataatcgtgtgacctacggtggagccatagacgctcaggacgccaaaaccttcgggccccaaaatgaagagaattgggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggcggcaccgaccctttggcacagtatgataaccctcgatatatgcgttattagcacactggatgccacctcggtatctgcgagtcctgcggttttggaagcccggggttttacttctcttaacccttgtatggggtcgagtgctttttgtcggtacggtacggccagtgctggaccgccgtggctgggaaaccgtgtcatactattgggagctatatacgApaIPspOMIhChR2 (H134R)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tttggcggcgtgaaaaaatgtgttcgcgccatagcacaatccaagacaaaagaagatgactttaacatagccggttgccatggcgctggtagccccccacacgatagtcccgatgtctgagacaaggagtcccatggttctcctgctgtagtcgttgctcaggccggtgaggttgaaaccgccgcacttttttacacaagcgcggtatcgtgttaggttctgttttcttctactgaaattgtatcggccaacggtaccgcgaccatcggggggtgtgctatcagggctacagactctgttcctcagggtaccaagaggacgacatcagcaacgagtccggccactccaachChR2 (H134R)1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ctcaggcggataaggatgacaggacaagtgagcagccactctgcatagcgcagccactgcacccggtgtcctgtggcaaggtagagcatagagggattcttaaactcaaaaaagaactcgagaatcaccttaaccatttcaatggcgcacacatagatctcctcccagccgcatggagtccgcctattcctactgtcctgttcactcgtcggtgagacgtatcgcgtcggtgacgtgggccacaggacaccgttccatctcgtatctccctaagaatttgagttttttcttgagctcttagtggaattggtaaagttaccgcgtgtgtatctagaggagggtcggcgtacTliIXhoIAlwNIBglIIBstAPIPaeR7IhChR2 (H134R)2,1202,1402,1602,1802,2002,2202,2402,260
    tagatttccaggtttggtaggcatagaacatcagcagcaaaatgctgaatcctgctgcaagccactgcaggacatttgacgcggtctgagcgccgttcgtgccgcgagattcaatccatccggcacagtaacattgatcctcagggaccaggacggacccgttcaccaccacaggatctaaaggtccaaaccatccgtatcttgtagtcgtcgttttacgacttaggacgacgttcggtgacgtcctgtaaactgcgccagactcgcggcaagcacggcgctctaagttaggtaggccgtgtcattgtaactaggagtccctggtcctgcctgggcaagtggtggtgtccPflFIBsu36ITth111IhChR2 (H134R)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    attagtaacgaacaaaagttcgcgtccgacggcagacaaagcgccgccatagtccatgggaccggggttttcttccacgtctcctgcttgctttaacagagagaagttcgtggctccggagccgggcgcgcccacgttgatgtcgatctggtccagggggatgtactcgccctcgtaatcattgcttgttttcaagcgcaggctgccgtctgtttcgcggcggtatcaggtaccctggccccaaaagaaggtgcagaggacgaacgaaattgtctctcttcaagcaccgaggcctcggcccgcgcgggtgcaactacagctagaccaggtccccctacatgagcgggagcAscIBmgBIBsaBIBspEIhChR2 (H134R)p2ATS2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ctggtgatcctgctcttatcatcagccggggtcccagaagcagatggaacatccaaaatgctgccggacacgacagattcgtttgacgtcagatagttcaggagcaagaatgcgaaaatgtactttgccacgatatccaggaaagaatatccccagctcgtcaccccaacgggcagaccactaggacgagaatagtagtcggccccagggtcttcgtctaccttgtaggttttacgacggcctgtgctgtctaagcaaactgcagtctatcaagtcctcgttcttacgcttttacatgaaacggtgctataggtcctttcttataggggtcgagcagtggggttgcccgtBsmIKflIPpuMINpHRTS2,6402,6602,6802,7002,7202,7402,7602,7802,800
    acaccgcgataccctcgactccaagagcccacacaattggataccccagccacatcactactgtcaacagcttcagggtattgaacatgtcagcggttcccgcggctttggcgtcctgtgcccactccaccagcaggatatacaagaccaccagaaagcatgcacaactgatagctgtggcgctatgggagctgaggttctcgggtgtgttaacctatggggtcggtgtagtgatgacagttgtcgaagtcccataacttgtacagtcgccaagggcgccgaaaccgcaggacacgggtgaggtggtcgtcctatatgttctggtggtctttcgtacgtgttgactatcgMfeIPciISphINpHR2,8202,8402,8602,8802,9002,9202,9402,960
    gtaccagaaccatctcatgaggtgggaggaggtagtcagggccgcggcaaggccagtcacgcacatagcgatatcgaaagtgatagctgtgaagagctttgtagcattgcttccggccaggagacccagagcgaggagaatcatgggcgtggaaagtgcccaggtgagataccgtcatggtcttggtagagtactccaccctcctccatcagtcccggcgccgttccggtcagtgcgtgtatcgctatagctttcactatcgacacttctcgaaacatcgtaacgaaggccggtcctctgggtctcgctcctcttagtacccgcacctttcacgggtccactctatggcaBarISapIBspQINpHR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccccacatggtgactacaccatctacctcttctcctcccagcatcactgagctgccttctgcaaaatggcccgctggcatttcaagaacggaaattgtcaggccgctcgccaatccagtgtaggaggcaatgctgacgacaggcacaaggatggttgacacagcaataagttttgggggtgtaccactgatgtggtagatggagaagaggagggtcgtagtgactcgacggaagacgttttaccgggcgaccgtaaagttcttgcctttaacagtccggcgagcggttaggtcacatcctccgttacgactgctgtccgtgttcctaccaactgtgtcgttattcaaaacNpHR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cccgtggatcatcgagtcctcgggtcataaaaacgaacagcagtatactcagtcctgcaagtgcgatgttgatatagagactgcttgcaagcaaagggtcgttcagcacgaactcgaacaactccctttgggtaacctcggcttgaagggccacggcactctcggtcacgggagggggcacctagtagctcaggagcccagtatttttgcttgtcgtcatatgagtcaggacgttcacgctacaactatatctctgacgaacgttcgtttcccagcaagtcgtgcttgagcttgttgagggaaacccattggagccgaacttcccggtgccgtgagagccagtgccctccAccIBstEIIBstZ17INpHR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cagggtctctgtcatggtggcgctagcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcttatcgataatcaagtcccagagacagtaccaccgcgatcgtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaatacttaagctatagttcgaatagctattagttBmtIClaINheIBspDIEcoRIHindIIIlox2722loxPWPRENpHR3,5203,5403,5603,5803,6003,6203,6403,660
    cctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaWPRE3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcgccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagcPflMIWPRE3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttcgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaWPRE4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattatttAfeIBplIBplIClaITliIXhoIBglIIBspDIPaeR7IhGH polyAWPRE4,2204,2404,2604,2804,3004,3204,3404,360
    attaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggCspCIhGH polyA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgcacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgBlpINsiISphIBbvCIhGH polyA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaaAloIPmlIBsaAIRsrIIBstEIIR-ITRhGH polyA4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtcgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcaKasINarISbfISfoIPluTIR-ITRf1 Origin4,9204,9404,9604,9805,0005,0205,0405,060
    gaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtf1 Origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgagcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactAloIPsiIBsaAIf1 Origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagcaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcg5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaaca5,6205,6405,6605,6805,7005,7205,7405,760
    ttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcattcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgtAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    actcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctatgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgatPvuIScaIAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    accgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaFspIAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    taatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacgggattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccAseIAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    aaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgattttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggctpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagtccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttcAlwNIpUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    acgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagpUC ori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgapUC ori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ggccttttgctcacatgtccggaaaacgagtgtacaPciIpUC ori7,540
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7543 bp550060006500700050010001500hChR2 (H134R)L-ITR,EF1 promoter,SbfI,MluI,AsiSI,PvuI,MfeI,EcoNI,HindIII,EcoNI,Bsu36Ilox2722,CspCI,KasI,NarI,SfoI,PluTI,BamHI,XbaI,SalI,AccI,BspEIloxPAscI,BsrGIEYFPPshAI,BfuAI,BspMIPspOMIApaIScaIAmpR,PvuIFspIAseIpUC ori,AlwNI,PciI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    7543