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pSPLp10
  • Sequence Map
Editing disabled on read-only sequence.
GAATTCTGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGTTAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAAACTTAAAACCTTAGAGGCTATCTTAAGACAGTCGGCAATTCACAAGGACACAGTGAGTTTTAACGAAACTCTCCGAGATTCCCGAAGAGTCACGCAATGTAGGGACCGAACAACAGGTGTTGGCAATTTGGAATTTTCGAAATTTTCGGAATATATAAGAAAAAAAAGAATATTTTGAATTTTGGAATCTCCGATAHindIII20406080100120140160
TTAAGTTGCTGATTTATATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAGCCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGAGAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAAATTCAACGACTAAATATAATTAAAATAACAAGTTTGTACTCTCGAATCATGCACTTTGTACTCTCGAATCATGCAATCGGTACTCTCGAATCATGCAATCGGTACTCCCAAATCAAGCAATTTGTACTCTCGAATCATGCAATTTGTACTCTCGAATCATGCACTTTGTACTCT180200220240260280300320340
GCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCAACAGATCCTCTACGCGGCCGCGGTACCATAACTTCGTATAGCATACATTATACGAAGTTATCTGGTTTAAACGTACCCGTAGTGGCTATGGCAGGGCTTGCCGCCCCGACGTTGGCTGCGAGCCCTGGGCCTTCACCCGAATCATGCATGATAGTTGTCCAACTTGACGACTAGTTGTCTAGGAGATGCGCCGGCGCCATGGTATTGAAGCATATCGTATGTAATATGCTTCAATAGACCAAATTTGCATGGGCATCACCGATACCGTCCCGAACGGCGGGGCTGCAACCGACGCTCGGGACCCGGAAGTGGBanIBarIEagIKpnINotIPasIPmeISacIISnaBIAcc65IHSV-1LoxP360380400420440460480500520
CGAACTTGGGGGTTGGGGTGGGGAAAAGGAAGAAACGCGGGCGTATTGGTCCCAATGGGGTCTCGGTGGGGTATCGACAGAGTGCCAGCCCTGGGACCGAACCCCGCGTTTATGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTCTTTTTATTGCCGTCATAGCGCGGGTGCTTGAACCCCCAACCCCACCCCTTTTCCTTCTTTGCGCCCGCATAACCAGGGTTACCCCAGAGCCACCCCATAGCTGTCTCACGGTCGGGACCCTGGCTTGGGGCGCAAATACTTGTTTGCTGGGTTGTGGGCACGCAAAATAAGACAGAAAAATAACGGCAGTATCGCGCCCABsaIPasIHSV-1540560580600620640660680700
TCCTTCCGGTATTGTCTCCTTCCGTGTTTCAGTTAGCCTCCCCCATCTCCCGGTACCGCATGCTATGCATCAGCTGCTAGCACCATGGCTCGAGATCCCGGGTGATCAAGTCTTCGTCGAGTGATTGTAAATAAAATGTAATTTACAGTATAGTATTTTAATTAATATACAAATGAGGAAGGCCATAACAGAGGAAGGCACAAAGTCAATCGGAGGGGGTAGAGGGCCATGGCGTACGATACGTAGTCGACGATCGTGGTACCGAGCTCTAGGGCCCACTAGTTCAGAAGCAGCTCACTAACATTTATTTTACATTAAATGTCATATCATAAAATTAATTATATGTTTACBanIBbsIBmtIKpnINcoINheINsiIPacISmaISphITliIXhoIXmaITspMIAcc65IPaeR7IHSV-1720740760780800820840860
ATTTGATAATAATTCTTATTTAACTATAATATATTGTGTTGGGTTGAATTAAAGGTCCGTATACTAGTATCGATGATTGACCCCAACAAAAGATTTATAATTAATCATAATCACGAACAACAACAAGTCAATGAAACAAATAAACAAGTTGTCGATAAAACATTCATAAATGACATAAACTATTATTAAGAATAAATTGATATTATATAACACAACCCAACTTAATTTCCAGGCATATGATCATAGCTACTAACTGGGGTTGTTTTCTAAATATTAATTAGTATTAGTGCTTGTTGTTGTTCAGTTACTTTGTTTATTTGTTCAACAGCTATTTTGTAAGTATTTACTGTClaISpeIBspDIBstZ17Ihr18809009209409609801,0001,0201,040
CAGCAACATACAATTCTTGCATAATAAAAATTTAAATGACATCATATTTGAGAATAACAAATGACATTATCCCTCGATTGTGTTTTACAAGTAGAATTCTACCCGTAAAGCGAGTTTAGTTTTGAAAAACAAATGACATCATTTGTATAATGACATCATCCCCTGATTGTGTTTTGTCGTTGTATGTTAAGAACGTATTATTTTTAAATTTACTGTAGTATAAACTCTTATTGTTTACTGTAATAGGGAGCTAACACAAAATGTTCATCTTAAGATGGGCATTTCGCTCAAATCAAAACTTTTTGTTTACTGTAGTAAACATATTACTGTAGTAGGGGACTAACACAAAASwaIhr11,0601,0801,1001,1201,1401,1601,1801,2001,220
ACAAGTAGAATTCTATCCGTAAAGCGAGTTCAGTTTTGAAAACAAATGAGTCATACCTAAACACGTTAATAATCTTCTGATATCAGCTTATGACTCAAGTTATGAGCCGTGTGCAAAACATGAGATAAGTTTATGACATCATCCACTGATCGTGCGTTACAAGTAGAATTCTACTTGTTCATCTTAAGATAGGCATTTCGCTCAAGTCAAAACTTTTGTTTACTCAGTATGGATTTGTGCAATTATTAGAAGACTATAGTCGAATACTGAGTTCAATACTCGGCACACGTTTTGTACTCTATTCAAATACTGTAGTAGGTGACTAGCACGCAATGTTCATCTTAAGATGABpuEIEcoRVhr11,2401,2601,2801,3001,3201,3401,3601,3801,400
CGTAAAGCCAGTTCGGTTATGAGCCGTGTGCAAAACATGACATCAGCTTATGACTCATACTTGATTGTGTTTTACGCGTAGAATTCTACTCGTAAAGCGAGTTCGGTTATGAGCCGTGTGCAAAACATGACATCAGCTTATGAGTCATAATTAATCGTGCGTTACAAGTAGAATTGCATTTCGGTCAAGCCAATACTCGGCACACGTTTTGTACTGTAGTCGAATACTGAGTATGAACTAACACAAAATGCGCATCTTAAGATGAGCATTTCGCTCAAGCCAATACTCGGCACACGTTTTGTACTGTAGTCGAATACTCAGTATTAATTAGCACGCAATGTTCATCTTAAMluIhr11,4201,4401,4601,4801,5001,5201,5401,560
CTACTCGTAAAGCGAGTTGAAGGATCATATTTAGTTGCGTTTATGAGATAAGATTGAAAAGCGTGTAAAATGTTTCCCGCGCTTGGCACAACTATTTACAATGCGGCCAAGTTATAAAAGATTCTAATCTGATATGTTTTAAAACACCTTTGCGGCCCGAGTTGTTTGCGTACGTGATGAGCATTTCGCTCAACTTCCTAGTATAAATCAACGCAAATACTCTATTCTAACTTTTCGCACATTTTACAAAGGGCGCGAACCGTGTTGATAAATGTTACGCCGGTTCAATATTTTCTAAGATTAGACTATACAAAATTTTGTGGAAACGCCGGGCTCAACAAACGCATGCABcgIBsaBIBsiWIhr11,5801,6001,6201,6401,6601,6801,7001,7201,740
GACTAGCGAAGAAGATGTGTGGACCGCAGAACAGATAGTAAAACAAAACCCTAGTATTGGAGCAATAATCGATCCTAGAATCCCAAAACAAACTGGTTATTGTGGTAGGTCATTTGTTTGGCAGAAAGAAAACTCGAGAAATTTCTCTGGCCGTTATTCGTTATTCTCTCTTTTCCTGATCGCTTCTTCTACACACCTGGCGTCTTGTCTATCATTTTGTTTTGGGATCATAACCTCGTTATTAGCTAGGATCTTAGGGTTTTGTTTGACCAATAACACCATCCAGTAAACAAACCGTCTTTCTTTTGAGCTCTTTAAAGAGACCGGCAATAAGCAATAAGAGAGAAAAGClaITliIXhoIBspDIPaeR7IHSP70hr11,7601,7801,8001,8201,8401,8601,8801,9001,920
TTTTTGGGTCTCTCCCTCTCTGCACTAATGCTCTCTCACTCTGTCACACAGTAAACGGCATACTGCTCTCGTTGGTTCGAGAGAGCGCGCCTCGAATGTTCGCGAAAAGAGCGCCGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACAATTCAATTCAAACAAGCAAAGTGAAAAACCCAGAGAGGGAGAGACGTGATTACGAGAGAGTGAGACAGTGTGTCATTTGCCGTATGACGAGAGCAACCAAGCTCTCTCGCGCGGAGCTTACAAGCGCTTTTCTCGCGGCCTCATATTTATCTCCGCGAAGCAGATGCCTCGCTGTTAAGTTAAGTTTGTTCGTTTCACBplIBplIBsaIBsgINruIHSP701,9401,9601,9802,0002,0202,0402,0602,0802,100
AACACGTCGCTAAGCGAAAGCTAAGCAAATAAACAAGCGCAGCTGAACAAGCTAAACAATCTGCAGTAAAGTGCAAGTTAAAGTGAATCAATTAAAAGTAACCAGCAACCAAGTAAATCAACTGCAACTACTGAAATCTGCCAAGAAGTAATTATTGAATACAAGAAGAGAACTCTTGTGCAGCGATTCGCTTTCGATTCGTTTATTTGTTCGCGTCGACTTGTTCGATTTGTTAGACGTCATTTCACGTTCAATTTCACTTAGTTAATTTTCATTGGTCGTTGGTTCATTTAGTTGACGTTGATGACTTTAGACGGTTCTTCATTAATAACTTATGTTCTTCTCTTGAGAjuIEarIPstIBmgBIHSP702,1202,1402,1602,1802,2002,2202,2402,260
TGAATAGGGAATTGGTAATACGACTCACTATAGGGGGATCCCGGTCCGAAGCGCGCGGAATTCAAAGGCCTACGTCGACGAGCTCACTAGTCGCGGCCGCTTTCGAATCTAGAATGAATCGTTTTTAAAATAACAAATCAATTGTTTTATAATATTCGTACGATTCTTTGATTATACTTATCCCTTAACCATTATGCTGAGTGATATCCCCCTAGGGCCAGGCTTCGCGCGCCTTAAGTTTCCGGATGCAGCTGCTCGAGTGATCAGCGCCGGCGAAAGCTTAGATCTTACTTAGCAAAAATTTTATTGTTTAGTTAACAAAATATTATAAGCATGCTAAGAAACTAATAEagINotISacISalISpeISspIStuIXbaIBamHIBsiWIBstBIRsrIISgrDIEco53kI"Multiple cloning site"p10""T7 promoter""HSP702,2802,3002,3202,3402,3602,3802,4002,4202,440
GTAATAAAATGTGATCATTAGGAAGATTACGAAAAATATAAAAAATATGAGTTCTGTGTGTATAACAAATGCTGTAAACGCCACAATTGTGTTTGTTGCAAATAAACCCATGATTATTTGATTAAAATTGTTGTTTTCTTTGTTCATAGACAATAGTGTGTTTTGCCTAAACGTGCATTATTTTACACTAGTAATCCTTCTAATGCTTTTTATATTTTTTATACTCAAGACACACATATTGTTTACGACATTTGCGGTGTTAACACAAACAACGTTTATTTGGGTACTAATAAACTAATTTTAACAACAAAAGAAACAAGTATCTGTTATCACACAAAACGGATTTGCACAleITatIp102,4602,4802,5002,5202,5402,5602,5802,6002,620
TACTGCATAAACTCCATGCGAGTGTATAGCGAGCTAGTGGCTAACGCTTGCCCCACCAAAGTAGATTCGTCAAAATCCTCAATTTCATCACCCTCCTCCAAGTTTAACATTTGGCCGTCGGAATTAACTTCTAAAGATGCCACATAATCTAATAAATGAAATAGAGATTCAAACGATGACGTATTTGAGGTACGCTCACATATCGCTCGATCACCGATTGCGAACGGGGTGGTTTCATCTAAGCAGTTTTAGGAGTTAAAGTAGTGGGAGGAGGTTCAAATTGTAAACCGGCAGCCTTAATTGAAGATTTCTACGGTGTATTAGATTATTTACTTTATCTCTAAGTTTGCMmeIBseRIp102,6402,6602,6802,7002,7202,7402,7602,7802,800
TGGCGTCATCGTCCGTTTCGACCATTTCCGAAAAGAACTCGGGCATAAACTCTATGATTTCTCTGGACGTGGTGTTGTCGAAACTCTCAAAGTACGCAGTCAGGAACGTGCGCGACATGTCGTCGGGAAACTCGCGCGGAAACATGTTGTTGTAACCGAACGGGTCCCATAGCGCACCGCAGTAGCAGGCAAAGCTGGTAAAGGCTTTTCTTGAGCCCGTATTTGAGATACTAAAGAGACCTGCACCACAACAGCTTTGAGAGTTTCATGCGTCAGTCCTTGCACGCGCTGTACAGCAGCCCTTTGAGCGCGCCTTTGTACAACAACATTGGCTTGCCCAGGGTATCGCGAhdIKflIPciIPciIBmgBIBsaHIPpuMIEcoO109Ip102,8202,8402,8602,8802,9002,9202,9402,960
CAAAACCAAATCTGCCAGCGTCAATAGAATGAGCACGATGCCGACAATGGAGCTGGCTTGGATAGCGATTCGAGTTAACAAGCTTGTCGAGAAGTACTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACGTTTTGGTTTAGACGGTCGCAGTTATCTTACTCGTGCTACGGCTGTTACCTCGACCGAACCTATCGCTAAGCTCAATTGTTCGAACAGCTCTTCATGATCTCCTAGTATTAGTCGGTATGGTGTAAACATCTCCAAAATGAACGAAATTTTTTGGAGGGTGTGGAGGGGGACTTGHpaIScaITatIBsaBIHindIIIp10SV40"SV40 late polyA"2,9803,0003,0203,0403,0603,0803,1003,1203,140
CTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGATCACGACTTTGTATTTTACTTACGTTAACAACAACAATTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCTAGACTAGTGBsmIBsmIHpaISV40"SV40 late polyA"""T7 terminator""3,1603,1803,2003,2203,2403,2603,2803,3003,320
TGCTTGAGCCTAGAAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTATCATAACCCCTAGGAGTCCAAGCGAGCTCGATATCCGTCGGCTTGAACGAATTGTTAGACATTATTTGCCGACTACCTTGGTGATCTCGCCTTTACGAACTCGGATCTTCTAGGCCGACGATTGTTTCGGGCTTTCCTTCGACTCAACCGACGACGGTGGCGACTCGTTATTGATAGTATTGGGGATCCTCAGGTTCGCTCGAGCTATAGGCAGCCGAACTTGCTTAACAATCTGTAATAAACGGCTGATGGAACCACTAGAGCGGAAASacIAvrIIBpuEIEcoRVEco53kISmR""..."3,3403,3603,3803,4003,4203,4403,4603,4803,500
CACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGGTGCATCACCTGTTTAAGAAGGTTGACTAGACGCGCGCTCCGGTTCGCTAGAAGAAGAACAGGTTCTATTCGGACAGATCGAAGTTCATACTGCCCGACTATGACCCGGCCGTCCGCGAGGTAACGGGTCAGCCGTCGCTGTAGGAAGCCGCGCTAAAACGGCCAATGACGCGACBglIMmeINaeIBsrDIDraIIINgoMIVSmR3,5203,5403,5603,5803,6003,6203,6403,660
TACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTATGGTTTACGCCCTGTTGCATTCGTGATGTAAAGCGAGTAGCGGTCGGGTCAGCCCGCCGCTCAAGGTATCGCAATTCCAAAGTAAATCGCGGAGTTTATCTAGGACAAGTCCTTGGCCTAGTTTCTCAAGGAGGCGGCGACCTGGATGGTTCCGTTGCGATACAAGAGAACGAAEciIBtgZISmR3,6803,7003,7203,7403,7603,7803,8003,8203,840
TTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCAACAGTCGTTCTATCGGTCTAGTTACAGCTAGCACCGACCGAGCTTCTATGGACGTTCTTACAGTAACGCGACGGTAAGAGGTTTAACGTCAAGCGCGAATCGACCTATTGCGGTGCCTTACTACAGCAGCACGTGTTGTTACCACTGAAGATGTCGCGCCTCTTAGAGCGAGAGAjuIBpmIPvuIApaLIBaeGIBfuAIBspMIBsrDISmR3,8603,8803,9003,9203,9403,9603,9804,0004,020
TCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGAGGTCCCCTTCGGCTTCAAAGGTTTTCCAGCAACTAGTTTCGAGCGGCGCAACAAAGTAGTTCGGAATGCCAGTGGCATTGGTCGTTTAGTTATAGTGACACACCGAAGTCCGGCGGTAGGTGACGCCTCGGCATGTTTACATGCCGGTCGTTGCAGCCAAGCTCTACCGCGAGCAcuIBstEIIDraIIISmR4,0404,0604,0804,1004,1204,1404,1604,1804,200
ATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATGATGTTTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGTACTGCGGTTGATGGAGACTATCAACTCAGCTATGAAGCCGCTAGTGGCGAAGGGAGTACTACAAATTGAAACAAAATCCCGCTGACGGGACGACGCATTGTAGCAACGACGAGGTATTGTAGTTTGTAGCTGGGTGCCGCATTGCGCGAACGACGAACCTACGGGCTCCGTATCBcgIBsaHIBspHISmR4,2204,2404,2604,2804,3004,3204,3404,360
ACTGTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTACCGGTTTATTGACTACCGGAAGCAGTGTGACCGTGTGCTTCTCAAATGCCTGAGGCCAGTTTTGACATGGGGTTTTTTTGTCAGTATTGTTCGGTACTTTTGGCGGTGACGCGGCAATGGTGGCGACGCAAGCCAGTTCCAAGACCTGGTCAACGCACTCGCGTATGCGATGAATGGCCAAATAACTGATGGCCTTCGTCACACTGGCACACGAAGAGTTTACGGACTCCGGTCAAAAgeIBsu36I4,3804,4004,4204,4404,4604,4804,5004,5204,540
GCTCAGGCTCTCCCCGTGGAGGTAATAATTGACGATATGATCATTTATTCTGCCTCCCAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAAGCTCGAGTCCGAGAGGGGCACCTCCATTATTAACTGCTATACTAGTAAATAAGACGGAGGGTCGACTGTAAGTAGGCCCCAGTCGTGGCAAAGACGCCTGACCGAAAGATGCACAAGGCGAAGGAAATCGTCGGGAACGCGGGACTCACGAACGCCGTCGCACTTCGABseYIBpu10I4,5604,5804,6004,6204,6404,6604,6804,700
  • Plasmid
Editing disabled on read-only sequence.
4715 bp350037504000425045002505007501000hr1SmRHSV-1LoxP

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4715