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s23_U6_sgRNA_scaffoldv2
  • Sequence Map
    Editing disabled on read-only sequence.
    TCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACGAATTGGCCAGCGCTGCATTACCCTGTTATCCCTAACCGGTGCGTTTTTATGCTTGTAGTATTAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGCTTAACCGGTCGCGACGTAATGGGACAATAGGGATTGGCCACGCAAAAATACGAACATCATAAAfeIAgeIEaeIEarIMscISspIBcoDIBsmAIBspHII-SceIseq2I-SceI20406080100120140160
    GTATAATGTTTTTAAGATCCGGAGttctgcAATTCATGtGATCTgcgcctgggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgCATATTACAAAAATTCTAGGCCTCaagacgTTAAGTACaCTAGAcgcggaccctcgagtgcccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggcPasISacIBspEIRsrIIEco53kI2x cHS4seq2180200220240260280300320340
    agccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaagctaggctgaaagaGGATCTtagaatgacacgcgcctgggagctcacggggacagccctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgcccctttttcgatccgactttctCCTAGAatcttactgtgcgcggaccctcgagtgcccctgtcgggPstISacISrfIEco53kI2x cHS4360380400420440460480500520
    ccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagaPasISrfIRsrII2x cHS4540560580600620640660680700
    cgctgctctttgagcctgcagacacctggggggatacggggaaaaagctGGATCataaCTCGAcgtgcaTACTgctggccttttgctcacatgtgagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgcgacgagaaactcggacgtctgtggacccccctatgcccctttttcgaCCTAGtattGAGCTgcacgtATGAcgaccggaaaacgagtgtacactcccggataaagggtactaaggaagtataaacgtatatgctatgttccgacaatctctctattaaccttaattaaactgaNspIPciIPstITfiIBmgBIAflIIIEcoO109IhU6_promoter2x cHS4720740760780800820840860
    gtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttGTGGAAAGGACGAAACACCggGTCTTcatttgtgtttctataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgatagtatacgaatggcattgaactttcataaagctaaagaaccgaaatatatagaaCACCTTTCCTGCTTTGTGGccCAGAABbsINdeIRsaITatIBsaAICviQIhU6_promoter8809009209409609801,0001,0201,040
    CgaGAAGACctgtttcagagctatgctggaaacagcatagcaagttgaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttTTTTgttttagagctagaaatagcaagttaaaataaggctagtccgtTTTTagcgcgcgctGGTCTAATACCCAATGctCTTCTGgacaaagtctcgatacgacctttgtcgtatcgttcaactttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaAAAAcaaaatctcgatctttatcgttcaattttattccgatcaggcaAAAAtcgcgcgcgaCCAGATTATGGGTTABanIBbsIBssHIIBssHIIsgRNA scaffold v2seq31,0601,0801,1001,1201,1401,1601,1801,2001,220
    CTCTCGTCTTATCCAGATGTTTTATACGCCTTAATTAATAGGGATAACAGGGTAATATCTTGCCCTGCACGAATACCAGCGACCCCTTGCCCAAATACTTGCCGTGGGCCTCGGCCTGAGAGCCAAAACACTTGATGCGGAAGAAGTCGGTGCGCTCCTGCTTGTCGCCGGCATCGAGAGCAGAATAGGTCTACAAAATATGCGGAATTAATTATCCCTATTGTCCCATTATAGAACGGGACGTGCTTATGGTCGCTGGGGAACGGGTTTATGAACGGCACCCGGAGCCGGACTCTCGGTTTTGTGAACTACGCCTTCTTCAGCCACGCGAGGACGAACAGCGGCCGTAGBsgIBtgIPacIBceAII-SceINmeAIIIRK2 trfaseq3I-SceI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GTTGCGCCACATCTAGGATCTGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCCAACGCGGTGTAGATCCTAGACGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGDrdIBssSIBssSαIColE1 originRK...a1,4201,4401,4601,4801,5001,5201,5401,560
    GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGMmeIApaLIBseYIColE1 origin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACMmeIAlwNIBceAIColE1 origin1,7601,7801,8001,8201,8401,8601,8801,9001,920
    AAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGAcuIColE1 origin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTABanIBspHIColE1 originAmpR2,1202,1402,1602,1802,2002,2202,2402,260
    AGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCTCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTTGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGAhdIBglIBmrIBpmIBsrDINmeAIIIAmpR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACAAclIFspIBsrDIAmpR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATCGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATABcgIBtsIBtsIEaeIPvuIRsaIScaITatIBtsαIBtsαICviQIAmpR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTAclIAcuIXmnIApaLIBsaHIBssSIBssSαIAmpR2,8202,8402,8602,8802,9002,9202,9402,960
    GCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAmpR2,9803,0003,0203,0403,060
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Assembly Parameters
    • Linear Map
    Editing disabled on read-only sequence.
    3072 bp2250250027502505007502x cHS4AmpRSeq2 F (DNA)Seq2 R (DNA)U6 (DNA)Seq3 F (DNA)pL1 Seq R (DNA)

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    3072