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pDF0694 Twinstrep-SUMO-Codon optimized DiCas7-11 H43A
  • Sequence Map
    Editing disabled on read-only sequence.
    TCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAori20406080100120140160
    TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTori180200220240260280300320340
    AGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCori360380400420440460480500520
    GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGori540560580600620640660680700
    TTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAPciISapIBspQI720740760780800820840860880
    TCACACCGCAATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAGTGTGGCGTTACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGATGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACPflFIBstZ17ITth111I9009209409609801,0001,0201,0401,060
    ACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGACGCCATTTCGAGTAGTCGCACCAGCACTTCGCTAAGTGTCTACAGACGGACAAGTAGGCGCAGGTCGAGCAACTCAAAGAGGTCTTCGCAATTACAGACCGAAGACTATTTCGBsmBIEsp3IROPROPpGEX_3_primer1,0801,1001,1201,1401,1601,1801,2001,2201,240
    GGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGCCCGGTACAATTCCCGCCAAAAAAGGACAAACCAGTGACTACGGAGGCACATTCCCCCTAAAGACAAGTACCCCCATTACTATGGCTACTTTGCTCTCTCCTACGAGTGCTATGCCCAATGACTACTACTTGTACGGGCCAATGACCTTGCAACACTCCCATTTGTTGACCGCCATACROPROP1,2601,2801,3001,3201,3401,3601,3801,4001,420
    GATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCTACGCCGCCCTGGTCTCTTTTTAGTGAGTCCCAGTTACGGTCGCGAAGCAATTATGTCTACATCCACAAGGTGTCCCATCGGTCGTCGTAGGACGCTACGTCTAGGCCTTGTATTACCACGTCCCGCGACTGAAGGCGCAAAGGTCTGAAATGCTTTGTGCCTTTGGCTTCTGGTAA1,4401,4601,4801,5001,5201,5401,5601,5801,600
    CATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGCCAGGACCCAACGCTGCCCGAGATGCGCCGTACAACAACGAGTCCAGCGTCTGCAAAACGTCGTCGTCAGCGAAGTGCAAGCGAGCGCATAGCCACTAAGTAAGACGATTGGTCATTCCGTTGGGGCGGTCGGATCGGCCCAGGAGTTGCTGTCCTCGTGCTAGTACGCGTGGGCACCGGTCCTGGGTTGCGACGGGCTCTACGCGGFspAI1,6201,6401,6601,6801,7001,7201,7401,7601,780
    GCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTCTGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCCGCACGCCGACGACCTCTACCGCCTGCGCTACCTATACAAGACGGTTCCCAACCAAACGCGTAAGTGTCAAGAGGCGTTCTTAACTAACCGAGGTTAAGAACCTCACCACTTAGGCAATCGCTCCACGGCGGCCGAAGGTAAGTCCAGCTCCACCGGGCCGAGGTACGTGGCGCTGCGtet (576 - 1190)1,8001,8201,8401,8601,8801,9001,9201,940
    AACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTTGCGCCCCTCCGTCTGTTCCATATCCCGCCGCGGATGTTAGGTACGGTTGGGCAAGGTACACGAGCGGCTCCGCCGTATTTAGCGGCACTGCTAGTCGCCAGGTTACTAGCTTCAATCCGACCATTCTCGGCGCTCGCTAGGAACTTCGACAGGGACTACCAGCAGTAGATGGACGGtet (576 - 1190)1,9601,9802,0002,0202,0402,0602,0802,1002,120
    TGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACGCCAGCAAGACGTAGCCCAGCGCGTCGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTACCTGTCGTACCGGACGTTGCGCCCGTAGGGCTACGGCGGCCTTCGCTCTTCTTAGTATTACCCCTTCCGGTAGGTCGGAGCGCAGCGCTTGCGGTCGTTCTGCATCGGGTCGCGCAGCCGGCGGTACGGCCGCTATTACCGGACGAAGAGCGGCTTTGCAAACCACCGCCCTGGTCANruIBstAPItet (576 - 1190)2,1402,1602,1802,2002,2202,2402,2602,2802,300
    GACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCTGCTTCCGAACTCGCTCCCGCACGTTCTAAGGCTTATGGCGTTCGCTGTCCGGCTAGTAGCAGCGCGAGGTCGCTTTCGCCAGGAGCGGCTTTTACTGGGTCTCGCGACGGCCGTGGACAGGATGCTCAACGTACTATTTCTTCTGTCAGTATTCACGCCGCTGCTATCAGTACGGGtet (576 - 1190)2,3202,3402,3602,3802,4002,4202,4402,4602,480
    CGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGGGCCACGGATTACTCACTCGATTGAATGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGClacItet (576 - 1190)2,5002,5202,5402,5602,5802,6002,6202,6402,660
    GTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGTCGTCCGCTTTTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGACAGAAHpaIBsmBIEsp3IlacI2,6802,7002,7202,7402,7602,7802,8002,8202,840
    CGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCGCCATAGCAGCATAGGGTGATGGCTCTATAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACGGGAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGEcoRVlacI2,8602,8802,9002,9202,9402,9602,9803,0003,020
    TTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATAAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTACGCTGGTCTACGAGGTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATApaIPspOMIlacI3,0403,0603,0803,1003,1203,1403,1603,1803,200
    CTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTAGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGCGCAACGCGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATMluIlacI3,2203,2403,2603,2803,3003,3203,3403,3603,380
    CCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGACAAACGGGCGGTCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGlacI3,4003,4203,4403,4603,4803,5003,5203,5403,560
    TTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTCTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGACTTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACBstAPIlacI3,5803,6003,6203,6403,6603,6803,7003,720
    CGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAAGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGGGTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTACCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTSphIEcoNItet (300 - 563)3,7403,7603,7803,8003,8203,8403,8603,8803,900
    CAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGTTCGCGAGTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGGTCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCTCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGtet (300 - 563)pBRrevB...primerT7T7_promoterlacOLacO3,9203,9403,9603,9804,0004,0204,0404,0604,080
    GGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCTGGAGCCATCCGCAGTTTGAAAAAGGTGGTGGTAGCGGTGGTGGTTCAGGTGGCCTATTGTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGGTACCCGTCGTCGGTAGTAGTAGTAGTAGTGTCGTCGCCGGACCACGGCGCGCCGTCGGTATACCGATCGACCTCGGTAGGCGTCAAACTTTTTCCACCACCATCGCCACCACCAAGTCCACCBarIBmtINcoINdeINheIXbaITranslation 4151-94066x His tag/ Twin StrepStrep Tag6xHIST7_tr..._RBSlacOLacO4,1004,1204,1404,1604,1804,2004,2204,2404,260
    TAGTGCATGGTCACACCCTCAGTTTGAGAAAATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCaAGATCAAAAAGAccACTCCTTTaAGAaggCTGATgATCACGTACCAGTGTGGGAGTCAAACTCTTTTACAGCCTGAGTCTTCAGTTAGTTCTTCGATTCGGTCTCCAGTTCGGTCTTCAGTTCGGACTCTGAGTGTAGTTAAATTTCCACAGGCTACCTAGAAGTCTCTAGAAGAAGtTCTAGTTTTTCTggTGAGGAAAtTCTtccGACTAcTranslation 4151-9406Sumo6x His tag/ Twin Strep4,2804,3004,3204,3404,3604,3804,4004,4204,440
    gAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATCCAAGCTGATCAGACCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCATGACCACTACCATGAAGATCAGCATCGcTTCGCAAGCGATTTTCTGTCCCATTCCTTTACCTGAGGAATTCTAAGAACATGCTGCCATAATCTTAGGTTCGACTAGTCTGGGGACTTCTAAACCTGTACCTCCTATTGCTATAATAACTCCGAGTGTCTCTTGTCTAACCACCTAGGTACTGGTGATGGTACTTCTAGTCGTAGCAflIIBamHITranslation 4151-9406SumoForwardgRAMP processing fwdTwist piece 1Translation 4601-9403sourceTranslation 4601-94064,4604,4804,5004,5204,5404,5604,5804,6004,620
    AGTTCTTGGAGCCCTTCCGCATGACGAAGTGGCAGGAGTCCACGCGCCGCAATAAGAACAATAAAGAGTTTGTGCGTGGCCAAGCGTTTGCCCGTTGGggcCGCAACAAGAAAGATAACACCAAGGGTCGTCCGtacATTACTGGTACGCTCCTGCGCTCAGCGGTGATTCGCTCCGCTCAAGAACCTCGGGAAGGCGTACTGCTTCACCGTCCTCAGGTGCGCGGCGTTATTCTTGTTATTTCTCAAACACGCACCGGTTCGCAAACGGGCAACCccgGCGTTGTTCTTTCTATTGTGGTTCCCAGCAGGCatgTAATGACCATGCGAGGACGCGAGTCGCCACTAAGCGAGGCGTranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94064,6404,6604,6804,7004,7204,7404,7604,7804,800
    GgagAACCTGCTGACACTGAGTGACGGTAAGATCTCCGAGAAAACCTGCTGCCCGGGGAAATTCGACACGGAAGATAAGGACCGTTTACTCCAGCTGCGTCAACGCTCCACGCTGCGTTGGACAGACAAGAATCCGTGTCCCGACAATGCGGAAACGTATTGTCCGTTCTGTGAGCTCCctcTTGGACGACTGTGACTCACTGCCATTCTAGAGGCTCTTTTGGACGACGGGCCCCTTTAAGCTGTGCCTTCTATTCCTGGCAAATGAGGTCGACGCAGTTGCGAGGTGCGACGCAACCTGTCTGTTCTTAGGCACAGGGCTGTTACGCCTTTGCATAACAGGCAAGACACTCGAGSacISmaIXmaITspMIDraIIIEco53kITranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94064,8204,8404,8604,8804,9004,9204,9404,9604,980
    CTCGGCCGCTCCGGAAATGACGGTAAGAAAGCAGAAAAGAAGgacTGGCGCTTCCGCATCCATTTTGGTaacCTTTCCCTGCCGGGCAAGCCCGACTTCGACGGTCCGAAGGCAATTGGTTCACAGCGCGTATTAAATCGTGTGGATTTTAAGTCGGGAAAGGCACATGACTTCTTCAGAGCCGGCGAGGCCTTTACTGCCATTCTTTCGTCTTTTCTTCctgACCGCGAAGGCGTAGGTAAAACCAttgGAAAGGGACGGCCCGTTCGGGCTGAAGCTGCCAGGCTTCCGTTAACCAAGTGTCGCGCATAATTTAGCACACCTAAAATTCAGCCCTTTCCGTGTACTGAAGAAGTMfeIPsrIRsrIITranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,0005,0205,0405,0605,0805,1005,1205,1405,160
    AGGCTTATGAGGTGGACCATACCCGCTTCCCTCGCTTTGAAGGGGAGATCACCATCGACAATAAAGTGTCCGCCGAGGCACGTAAGTTGCTGTGTGACAGCCTTAAATTCACGGATCGCCTGTGTGGAGCCCTGTGCGTCATCCGCTTCGACGAGTATACACCGGCCGCGGACAGCGGTCCGAATACTCCACCTGGTATGGGCGAAGGGAGCGAAACTTCCCCTCTAGTGGTAGCTGTTATTTCACAGGCGGCTCCGTGCATTCAACGACACACTGTCGGAATTTAAGTGCCTAGCGGACACACCTCGGGACACGCAGTAGGCGAAGCTGCTCATATGTGGCCGGCGCCTGTCGCCBstZ17ITranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,1805,2005,2205,2405,2605,2805,3005,3205,340
    TAAGCAAACCGAGAATGTGCAGGCAGAGCCCAACGCAAACCTGGCGGAGAAAACTGCCGAGCAAATTATCTCGATCCTCGATGACAACAAGAAAACGGAATACACCCGCCTTTTAGCGGACGCCATCCGCTCATTGCGTCGCTCCTCAAAACTTGTTGCAGGTTTGCCCAAGGATCACATTCGTTTGGCTCTTACACGTCCGTCTCGGGTTGCGTTTGGACCGCCTCTTTTGACGGCTCGTTTAATAGAGCTAGGAGCTACTGTTGTTCTTTTGCCTTATGTGGGCGGAAAATCGCCTGCGGTAGGCGAGTAACGCAGCGAGGAGTTTTGAACAACGTCCAAACGGGTTCCTAGTGTranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,3605,3805,4005,4205,4405,4605,4805,500
    GATGGTAAGGACGATCATTACTTGtggGACATTGGGAAGAAGAAGAAGGATGAGAATTCGGTTACCATCCGTCAGATTCTTACCACGAGTGCCGACACGAAGGAGCTGAAGAACGCTGGTAAGTGGCGCGAGTTTTGTGAGAAGCTTGGTGAGGCGCTTTATTTGAAGAGTAAGGATACTACCATTCCTGCTAGTAATGAACaccCTGTAACCCTTCTTCTTCTTCCTACTCTTAAGCCAATGGTAGGCAGTCTAAGAATGGTGCTCACGGCTGTGCTTCCTCGACTTCTTGCGACCATTCACCGCGCTCAAAACACTCTTCGAACCACTCCGCGAAATAAACTTCTCATTCCTATEcoRITranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,5205,5405,5605,5805,6005,6205,6405,6605,680
    TGAGCGGCGGGTTGAAGATTACACGCCGTATTCTCGGGGACGCAGAGTTTCATGGCAAGCCAGATCGCTTGGAAAAGTCCCGTAGTGTCTCGATCGGAAGCGTGTTAAAGGAAACCGTGGTGTGCGGAGAACTCGTCGCAAAGACCCCGTTCTTCTTTGGCGCGATCGACGAGGATGCACTCGCCGCCCAACTTCTAATGTGCGGCATAAGAGCCCCTGCGTCTCAAAGTACCGTTCGGTCTAGCGAACCTTTTCAGGGCATCACAGAGCTAGCCTTCGCACAATTTCCTTTGGCACCACACGCCTCTTGAGCAGCGTTTCTGGGGCAAGAAGAAACCGCGCTAGCTGCTCCTACGTranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,7005,7205,7405,7605,7805,8005,8205,8405,860
    AAAACAAACTgacTTACAGGTACTGCTTACTCCGGACAATAAGTATCGCCTGCCGCGCAGCGCCGTCCGCGGCATCCTTCGCCGCGATCTTCAAACGTACTTTGACAGTCCCTGTAATGCGGAGTTAGGCGGTCGTCCATGTATGTGCAAGACCTGTCGTATCATGCGTGGCATTACGTTTTGTTTGActgAATGTCCATGACGAATGAGGCCTGTTATTCATAGCGGACGGCGCGTCGCGGCAGGCGCCGTAGGAAGCGGCGCTAGAAGTTTGCATGAAACTGTCAGGGACATTACGCCTCAATCCGCCAGCAGGTACATACACGTTCTGGACAGCATAGTACGCACCGTAATGCTranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94065,8805,9005,9205,9405,9605,9806,0006,0206,040
    GTTATGgacGCGCGCTCCGAGTATAACGCGCCCCCGGAGATTCGTCACCGTACCCGCATTAATCCGTTTACGGGAACAGTTGCTGAGGGCGCCCTGTTTAATATGGAAGTGGCCCCGGAAGGGATCGTATTCCCATTCCAATTACGCTATCGCGGCTCTGAGGACGGGCTCCCAGACGCAATACctgCGCGCGAGGCTCATATTGCGCGGGGGCCTCTAAGCAGTGGCATGGGCGTAATTAGGCAAATGCCCTTGTCAACGACTCCCGCGGGACAAATTATACCTTCACCGGGGCCTTCCCTAGCATAAGGGTAAGGTTAATGCGATAGCGCCGAGACTCCTGCCCGAGGGTCTGCBbvCITranslation 4151-9406Twist piece 1Translation 4601-9403sourceTranslation 4601-94066,0606,0806,1006,1206,1406,1606,1806,2006,220
    CGCTCAAGACCGTGCTGAAGTGGTGGGCAGAGGGGCAAGCTTTTATGTCAGGGGCCGCCAGTACCGGGAAAGGCCGTTTTCGTATGGAGAATGCCAAATATGAAACCTTGGATCTGAGCGATGAGAATCAACGCAATGACTACTTGAAGAATTGGGGCTGGCGTGACGAGAAGGGTCTGCGAGTTCTGGCACGACTTCACCACCCGTCTCCCCGTTCGAAAATACAGTCCCCGGCGGTCATGGCCCTTTCCGGCAAAAGCATACCTCTTACGGTTTATACTTTGGAACCTAGACTCGCTACTCTTAGTTGCGTTACTGATGAACTTCTTAACCCCGACCGCACTGCTCTTCCCAGATranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 16,2406,2606,2806,3006,3206,3406,3606,3806,400
    TGAGGAGCTTAAGAAACGTTTAAATTCAGGTCTGCCGGAGCCTGGCAATTACCGTGACCCGAAGTGGCATGAAATCAACGTGTCAATCGAGATGGCGAGTCCCTTCATCAACGGCgacCCTATCCGCGCAGCTGTAGACAAGCGTGGGACGgacGTCGTGACCTTCGTTAAGTACAAGACTCCTCGAATTCTTTGCAAATTTAAGTCCAGACGGCCTCGGACCGTTAATGGCACTGGGCTTCACCGTACTTTAGTTGCACAGTTAGCTCTACCGCTCAGGGAAGTAGTTGCCGctgGGATAGGCGCGTCGACATCTGTTCGCACCCTGCctgCAGCACTGGAAGCAATTCATGTTCZraIAatIIAflIITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 26,4206,4406,4606,4806,5006,5206,5406,5606,580
    GCGGAAGGGGAAGAAGCAAAGCCCGTGTGCGCATATAAGGCGGAGTCGTTTCGCGGCGTCATTCGTTCGGCTGTAGCACGCATTCACATGGAAGATGGAGTGCCCCTGACTGAGCTTACTCACTCAGATTGCGAGTGTTTGCTCTGTCAAATCTTCGGGTCGGAATATGAGGCTGGTACGCCTTCCCCTTCTTCGTTTCGGGCACACGCGTATATTCCGCCTCAGCAAAGCGCCGCAGTAAGCAAGCCGACATCGTGCGTAAGTGTACCTTCTACCTCACGGGGACTGACTCGAATGAGTGAGTCTAACGCTCACAAACGAGACAGTTTAGAAGCCCAGCCTTATACTCCGACCATFspAITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 26,6006,6206,6406,6606,6806,7006,7206,7406,760
    AGATTCGTTTCGAAgatTTAGTATTTGAGAGTgacCCGGAGCCGGTCACCTTCGATCACGTTGCAATCgacCGCTTCACCGGCGGTGCCGCTGACAAGAAGAAGTTCGACGACAGTCCTTTGCCAGGGTCGCCGGCGCGCCCTCTGATGCTGAAGGGTTCCTTCTGGATTCGCCGTGATCTAAGCAAAGCTTctaAATCATAAACTCTCActgGGCCTCGGCCAGTGGAAGCTAGTGCAACGTTAGctgGCGAAGTGGCCGCCACGGCGACTGTTCTTCTTCAAGCTGCTGTCAGGAAACGGTCCCAGCGGCCGCGCGGGAGACTACGACTTCCCAAGGAAGACCTAAGCGGCACTAscIMreIEcoNITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 26,7806,8006,8206,8406,8606,8806,9006,9206,940
    CGTTCTCGAAGATGAGGAGTATTGCAAAGCGCTCGGGAAAGCATTAGCCGATGTAAATAATGGTTTATATCCTCTGGGTGGAAAATCCGCCATCGGATACGGACAAGTTAAGAGCCTGGGTATCAAGGGCGACGATAAGCGTATCAGCCGTTTAATGAATCCGGCCTTCGACGAAACCGCAAGAGCTTCTACTCCTCATAACGTTTCGCGAGCCCTTTCGTAATCGGCTACATTTATTACCAAATATAGGAGACCCACCTTTTAGGCGGTAGCCTATGCCTGTTCAATTCTCGGACCCATAGTTCCCGCTGCTATTCGCATAGTCGGCAAATTACTTAGGCCGGAAGCTGCTTTGGTranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 26,9606,9807,0007,0207,0407,0607,0807,1007,120
    GACGTGGCGGTTCCAGAGAAGCCTAAAACCGATGCCGAGGTCCGTATCGAAGCTGAGAAGGTATATTATCCCCACTACTTTGTGGAACCGCACAAGAAGGTAGAGCGCGAGGAGAAGCCGTGCGGTCATCAAAAGTTTCATGAGGGACGCCTTACCGGTAAGATTCGTTGTAAACTTACTGCACCGCCAAGGTCTCTTCGGATTTTGGCTACGGCTCCAGGCATAGCTTCGACTCTTCCATATAATAGGGGTGATGAAACACCTTGGCGTGTTCTTCCATCTCGCGCTCCTCTTCGGCACGCCAGTAGTTTTCAAAGTACTCCCTGCGGAATGGCCATTCTAAGCAACATTTGAATAgeIBmgBITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,1407,1607,1807,2007,2207,2407,2607,280
    TTACGAAAACGCCTCTCATTGTGCCGGACACCAGCAATGATGACTTCTTTCGCCCTGCGGACAAAGAGGCCCGCAAGGAGAAGGACGAGTATCACAAATCCTATGCCTTCTTTCGCCTGCATAAGCAAATCATGATCCCCGGTAGTGAACTCCGCGGGATGGTGTCCTCTGTTTACGAAATGCTTTTGCGGAGAGTAACACGGCCTGTGGTCGTTACTACTGAAGAAAGCGGGACGCCTGTTTCTCCGGGCGTTCCTCTTCCTGCTCATAGTGTTTAGGATACGGAAGAAAGCGGACGTATTCGTTTAGTACTAGGGGCCATCACTTGAGGCGCCCTACCACAGGAGACAAATGCTTranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,3007,3207,3407,3607,3807,4007,4207,4407,460
    AACGGTTACAAATTCATGTTTCCGTATCTTTGACGAGACTAAGCGTCTTAGTTGGCGCATGGATGCCGATCACCAAAACGTTTTACAAGATTTCTTACCGGGCCGTGTTACGGCCGACGGCAAGCACATTCAAAAGTTTTCCGAGACTGCACGTGTACCGTTTTATgacAAAACGCAATTGCCAATGTTTAAGTACAAAGGCATAGAAACTGCTCTGATTCGCAGAATCAACCGCGTACCTACGGCTAGTGGTTTTGCAAAATGTTCTAAAGAATGGCCCGGCACAATGCCGGCTGCCGTTCGTGTAAGTTTTCAAAAGGCTCTGACGTGCACATGGCAAAATActgTTTTGCGTTAhdIPmlITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,4807,5007,5207,5407,5607,5807,6007,6207,640
    AAGCACTTTGACATTCTCGACGAGCAGGAAATTGCCGGTGAAAAGCCGGTCCGCATGTGGGTGAAGCGTTTCATTAAGCGCCTGAGCCTGGTGGACCCTGCAAAGCACCCACAAAAGAAGCAAGACAATAAGTGGAAACGCCGCAAGGAAGGTATTGCGACGTTCATTGAGCAGAAGATTCGTGAAACTGTAAGAGCTGCTCGTCCTTTAACGGCCACTTTTCGGCCAGGCGTACACCCACTTCGCAAAGTAATTCGCGGACTCGGACCACCTGGGACGTTTCGTGGGTGTTTTCTTCGTTCTGTTATTCACCTTTGCGGCGTTCCTTCCATAACGCTGCAAGTAACTCGTCTTCTRsrIITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,6607,6807,7007,7207,7407,7607,7807,8007,820
    ATGGGTCTTACTATTTTAACGTGGTCACCAACAATGGCTGCACCAGCTTTCACTTGTGGCACAAGCCAGACAACTTCGACCAAGAGAAGTTAGAGGGCATCCAAAACGGGGAGAAGTTAgatTGCTGGGTTCGTGATAGCCGTTACCAAAAGGCATTTCAAGAAATCCCGGAGAACGATACCCAGAATGATAAAATTGCACCAGTGGTTGTTACCGACGTGGTCGAAAGTGAACACCGTGTTCGGTCTGTTGAAGCTGGTTCTCTTCAATCTCCCGTAGGTTTTGCCCCTCTTCAATctaACGACCCAAGCACTATCGGCAATGGTTTTCCGTAAAGTTCTTTAGGGCCTCTTGCTAjuITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 27,8407,8607,8807,9007,9207,9407,9607,9808,000
    TCCGGACGGATGGGAGTGTAAGGAAGGTTATCTGCATGTGGTGGGCCCTTCGAAGGTTGAATTTAGCGACAAGAAGGGCGATGTCATTAATAACTTCCAAGGGACTCTCCCATCAGTGCCCAATGATTGGAAAACGATTCGTACAAACGACTTTAAGAACCGCAAACGTAAGAACGAGAGGCCTGCCTACCCTCACATTCCTTCCAATAGACGTACACCACCCGGGAAGCTTCCAACTTAAATCGCTGTTCTTCCCGCTACAGTAATTATTGAAGGTTCCCTGAGAGGGTAGTCACGGGTTACTAACCTTTTGCTAAGCATGTTTGCTGAAATTCTTGGCGTTTGCATTCTTGCTCApaIPspOMITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 28,0208,0408,0608,0808,1008,1208,1408,1608,180
    CCCGTTTTCTGTTGCGAGGATGACAAGGGGAACTACTACACGATGGCCAAATATTGTGAAACGTTCTTCTTCGACCTGAAAGAGAACGAGGAGTATGAGATCCCCGAGAAGGCACGTATCAAGTACAAGGAGTTATTACGCGTGTACAACAACAATCCGCAAGCGGTTCCCGAGAGCGGGGCAAAAGACAACGCTCCTACTGTTCCCCTTGATGATGTGCTACCGGTTTATAACACTTTGCAAGAAGAAGCTGGACTTTCTCTTGCTCCTCATACTCTAGGGGCTCTTCCGTGCATAGTTCATGTTCCTCAATAATGCGCACATGTTGTTGTTAGGCGTTCGCCAAGGGCTCTCGCMluISspIBsrGITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 3Twi... 28,2008,2208,2408,2608,2808,3008,3208,3408,360
    TGTTCCAGAGTCGCGTAGCGCGCGAGAACGTAGAAAAGCTGAAGTCTGGGGATTTGGTGTATTTCAAGCACAACGAGAAATATGTTGAGGACATCGTACCGGTTCGTATTTCACGTACCGTAGACGACCGCATGATCGGAAAGCGTATGTCGGCTGATTTGCGTCCATGTCACGGTGAACAAGGTCTCAGCGCATCGCGCGCTCTTGCATCTTTTCGACTTCAGACCCCTAAACCACATAAAGTTCGTGTTGCTCTTTATACAACTCCTGTAGCATGGCCAAGCATAAAGTGCATGGCATCTGCTGGCGTACTAGCCTTTCGCATACAGCCGACTAAACGCAGGTACAGTGCCACTAgeITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,3808,4008,4208,4408,4608,4808,5008,5208,540
    CTGGGTAGAGGACGGGGACCTCTCTGCGTTGAATGCATATCCAGAGAAGCGCCTTTTATTGCGTCACCCTAAGGGCCTTTGCCCGGCATGTCGCCTTTTCGGAACGGGTAGCTACAAGGGCCGCGTTCGTTTTGGCTTCGCGAGCCTGGAGAACgacCCGGAGTGGTTGATTCCTGGAGACCCATCTCCTGCCCCTGGAGAGACGCAACTTACGTATAGGTCTCTTCGCGGAAAATAACGCAGTGGGATTCCCGGAAACGGGCCGTACAGCGGAAAAGCCTTGCCCATCGATGTTCCCGGCGCAAGCAAAACCGAAGCGCTCGGACCTCTTGctgGGCCTCACCAACTAAGGACCTNruINsiIBsu36ITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,5608,5808,6008,6208,6408,6608,6808,7008,720
    AAGAATCCCGGTGATCCATTCCACGGCGGGCCAGTGATGCTGAGCTTGCTGGAGCGCCCGCGTCCCACCTGGAGCATTCCTGGCAGTGATAATAAGTTCAAAGTTCCAGGCCGTAAGTTCTACGTTCACCATCATGCTTGGAAAACGATTAAGGACGGTAACCATCCGACCACCGGCATTCTTAGGGCCACTAGGTAAGGTGCCGCCCGGTCACTACGACTCGAACGACCTCGCGGGCGCAGGGTGGACCTCGTAAGGACCGTCACTATTATTCAAGTTTCAAGGTCCGGCATTCAAGATGCAAGTGGTAGTACGAACCTTTTGCTAATTCCTGCCATTGGTAGGCTGGTGGCCGTTranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,7408,7608,7808,8008,8208,8408,8608,8808,900
    AAGCCATTGAACAATCGCCAAATAACCGCACGGTTGAAGCGCTCGCCGGTGGCAACAGCTTCAGCTTCGAAATTGCGTTCGAAAATTTGAAGGAGTGGGAGTTGGGCCTGCTGATCCATAGCCTGCAGCTTGAAAAGGGCTTGGCCCATAAGCTCGGTATGGCTAAGAGCATGGGCTTTTCGGTAACTTGTTAGCGGTTTATTGGCGTGCCAACTTCGCGAGCGGCCACCGTTGTCGAAGTCGAAGCTTTAACGCAAGCTTTTAAACTTCCTCACCCTCAACCCGGACGACTAGGTATCGGACGTCGAACTTTTCCCGAACCGGGTATTCGAGCCATACCGATTCTCGTACCCGAAPstITranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 38,9208,9408,9608,9809,0009,0209,0409,060
    TGGCTCAGTAGAGATCGACGTTGAGAGTGTCCGCCTGCGTAAAGATTGGAAGCAGTGGCGCAATGGTAATTCAGAGATCCCAAATTGGTTGGGCAAGGGGTTTGCAAAGCTGAAGGAGTGGTTCCGCGACGAGCTGGACTTCATCGAGAATTTGAAGAAATTACTGTGGTTCCCCGAAACCGAGTCATCTCTAGCTGCAACTCTCACAGGCGGACGCATTTCTAACCTTCGTCACCGCGTTACCATTAAGTCTCTAGGGTTTAACCAACCCGTTCCCCAAACGTTTCGACTTCCTCACCAAGGCGCTGCTCGACCTGAAGTAGCTCTTAAACTTCTTTAATGACACCAAGGGGCTTTranslation 4151-9406Translation 4601-9403sourceTranslation 4601-9406Twist piece 39,0809,1009,1209,1409,1609,1809,2009,2209,240
    GGTGATCAAGCTCCGCGCGTATGTTACCCCATGCTTCGCAAGAAGGATGACCCTAATGGAAACTCAGGTTATGAGGAGTTGAAAGACGGCGAATTTAAGAAGGAAGATCGTCAGAAGAAGCTTACAACACCATGGACGCCGTGGGCTTGAGCGGCCGCACTCGAGGCCCGAAAGGAAGCCACTAGTTCGAGGCGCGCATACAATGGGGTACGAAGCGTTCTTCCTACTGGGATTACCTTTGAGTCCAATACTCCTCAACTTTCTGCCGCTTAAATTCTTCCTTCTAGCAGTCTTCTTCGAATGTTGTGGTACCTGCGGCACCCGAACTCGCCGGCGTGAGCTCCGGGCTTTCCTTCNcoINotITliIXhoIPspXIPaeR7ITranslation 4151-9406Translation 4601-9406Translation 4601-9403sourceTwist piece 39,2609,2809,3009,3209,3409,3609,3809,4009,420
    CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGGACTCAACCGACGACGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTATAGGGCGTTCTCCGGGCCGTCATGGCCGTATTGGTTCGGATACGGATGTCGTAGGTCCCACTGCCACGGCTCCEcoRVT7_terminatorT7_Ter...rimer9,4409,4609,4809,5009,5209,5409,5609,5809,600
    ATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGTACTGCTACTCGCGTAACAATCTAAAGTATGTGCCACGGACTGACGCAATCGTTAAATTGACACTATTTGATGGCGTAATTTCGAATAGCTACTATTCGACAGTTTGTACTCTTAAGAACTTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACClaIBspDIEcoRI9,6209,6409,6609,6809,7009,7209,7409,7609,780
    GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGSspIZraIAatIIAmpicillinAmpR_promoter9,8009,8209,8409,8609,8809,9009,9209,9409,960
    CCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTAmpicillin9,98010,00010,02010,04010,06010,08010,10010,12010,140
    AGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACTCAAGACGATACACCGCGCCATAATAGGGCACAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGScaIAmpicillin10,16010,18010,20010,22010,24010,26010,28010,30010,320
    ACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCATGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACGTCGTTACCGTTGTTGCAACGCGTPstIAmpicillin10,34010,36010,38010,40010,42010,44010,46010,48010,500
    AACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGBsaIAmpicillin10,52010,54010,56010,58010,60010,62010,64010,66010,680
    AGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAATCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTAhdIAmpicillin10,70010,72010,74010,76010,78010,80010,82010,840
    AGGATCTAGGTGAAGATCCTTTTTGATAATCTCCTAGATCCACTTCTAGGAAAAACTATTAG10,86010,880
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10889 bp800085009000950010000105005001000150020002500Translation 4151-9406Translation 4601-9406Translation 4601-9403sourcelacITwist piece 3ROPROPoriPciI,BspQI,SapIBstZ17IPflFIpGEX_3_primer,Tth111I,BsmBI,Esp3IFspAIBstAPINruItet (576 - 1190)BsmBI,Esp3ISspIMluI,BsrGI,AgeINsiI,Bsu36INruIPstIT7_terminator,T7_Terminal_primer,NcoI,NotI,PaeR7I,PspXI,TliI,XhoIEcoRV,BspDI,ClaIAmpR_promoter,EcoRI,ZraI,AatII,SspIScaIAmpicillin,PstI,BsaI,AhdI

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    10889