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EW171 CMV-TO-UTRN-43ZF-VP64 (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMV20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMV180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaNcoICspCISnaBICMV360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcBsmBIEsp3ISgrDICMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactCTAGCGTTTAAACTTAAGCTTCGCCACCATGTTCGAGTGTGCCGTCTGTGGGGAGTCCTTCGTAAATCCTGCAGAGTTGGCCGACCACGTTACAGTTCACACCGGGGAGAGACCCTTCGAGTGTGCCGTTTGTGGGGAGTCTTTttctgtggccctggctaggtcggaggcctgaGATCGCAAATTTGAATTCGAAGCGGTGGTACAAGCTCACACGGCAGACACCCCTCAGGAAGCATTTAGGACGTCTCAACCGGCTGGTGCAATGTCAAGTGTGGCCCCTCTCTGGGAAGCTCACACGGCAAACACCCCTCAGAAABbsIPmeIAflIIHindIIIMF2EC4AV6CG8ES10FV12NP14AE16LA18DH20VT22VH24TG26ER28PF30EC32AV34CG36ES38F760-1615UTRN Promoter Pos -43-Binding ZF (Q9GZU2 finger 9-Q9GZU...ger 1-Q9GZU2 finger 6-Q9GZU2 finger 9-O15156 finger 1)720740760780800820840860
    CGTCAATCCAGCCGAGCTCGCTGACCACGTCACAGTCCACACTGGGGAGCGACCTTACGAGTGTCAAGAGTGTCAAAAGACGTTCTCTAGGTCATCCCACTTGCTCCGTCACCAAAGTGTCCACTGTACTGGAGAGAGACCTTTCACGTGTGACTCATGTGGTTTCGGTTTCTCTGCAGTTAGGTCGGCTCGAGCGACTGGTGCAGTGTCAGGTGTGACCCCTCGCTGGAATGCTCACAGTTCTCACAGTTTTCTGCAAGAGATCCAGTAGGGTGAACGAGGCAGTGGTTTCACAGGTGACATGACCTCTCTCTGGAAAGTGCACACTGAGTACACCAAAGCCAAAGAGAAleIPmlIBstXIV40NP42AE44LA46DH48VT50VH52TG54ER56PY58EC60QE62CQ64KT66FS68RS70SH72LL74RH76QS78VH80CT82GE84RP86FT88CD90SC92GF94GF96S760-1615UTRN Promoter Pos -43-Binding ZF (Q9GZU2 finger 9-Q9GZU2 finger 9-Q96NJ6 finger 13-Q9Y2K1 finger 1-Q6ZNG0 finger 1-Q9GZU2 finger 6-Q9GZU2 finger 9-O15156 finger 1)8809009209409609801,0001,0201,040
    TGTGAGAAGCTCCTCGACGAGCACGTCTTGACGTGTACGGGCGAGCGTCCTTACGAGTGTGGGCAATGTGGACGGTACTTCATCCAAATGGCAGACTTCCACAGGCACGAGAAGTGTCACACAGGGGAGCGACCATACGAGTGTGAGGACTGTGGGTTGGGTTTCGTAGACTTAAACACTCTTCGAGGAGCTGCTCGTGCAGAACTGCACATGCCCGCTCGCAGGAATGCTCACACCCGTTACACCTGCCATGAAGTAGGTTTACCGTCTGAAGGTGTCCGTGCTCTTCACAGTGTGTCCCCTCGCTGGTATGCTCACACTCCTGACACCCAACCCAAAGCATCTGAATTBseRIC98EK100LL102DE104HV106LT108CT110GE112RP114YE116CG118QC120GR122YF124IQ126MA128DF130HR132HE134KC136HT138GE140RP142YE144CE146DC148GL150GF152VD154L760-1615UTRN Promoter Pos -43-Binding ZF (Q9GZU2 finger 9-Q9GZU2 finger 9-Q96NJ6 finger 13-Q9Y2K1 finger 1-Q6ZNG0 finger 1-Q9GZU2 finger 6-Q9GZU2 finger 9-O15156 finger 1)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CTGACTTAACTGACCACCAAAAGGTCCACACAGGGGAGAGACCATTCGAGTGTGCAGTCTGTGGAGAGTCCTTCGTAAATCCCGCCGAGCTTGCAGACCACGTTACAGTCCACACTGGGGAGAGGCCACAAGAGTGTCCAGTTTGTCACAAGATCATACACGGAGCTGGAAAACTGACTGAATTGACTGGTGGTTTTCCAGGTGTGTCCCCTCTCTGGTAAGCTCACACGTCAGACACCTCTCAGGAAGCATTTAGGGCGGCTCGAACGTCTGGTGCAATGTCAGGTGTGACCCCTCTCCGGTGTTCTCACAGGTCAAACAGTGTTCTAGTATGTGCCTCGACCTTTTGAAleIBsgIT156DL158TD160HQ162KV164HT166GE168RP170FE172CA174VC176GE178SF180VN182PA184EL186AD188HV190TV192HT194GE196RP198QE200CP202VC204HK206II208HG210AG212KL214760-1615UTRN Promoter Pos -43-Binding ZF (Q9GZU2 finger 9-Q9GZU2 finger 9-Q96NJ6 finger 13-Q9Y2K1 finger 1-Q6ZNG0 finger 1-Q9GZU2 finger 6-Q9GZU2 finger 9-O15156 finger 1)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TCCACGTCACATGAGGACGCACGCTGCTGCAGGCGGAAGCGGAGGCTCTGGCGGATCtgatgccctggacgacttcgacctggatatgctgggcagcgacgccctggatgattttgatctggacatgctgggatctgacgctctggacgatttcgatctcgacatgttgggatcaAGGTGCAGTGTACTCCTGCGTGCGACGACGTCCGCCTTCGCCTCCGAGACCGCCTAGactacgggacctgctgaagctggacctatacgacccgtcgctgcgggacctactaaaactagacctgtacgaccctagactgcgagacctgctaaagctagagctgtacaaccctagtPciIPR216HM218RT220HA222AA224GG226SG228GS230GG232SD234AL236DD238FD240LD242ML244GS246DA248LD250DF252DL254DM256LG258SD260AL262DD264FD266LD268ML270GS272760-1615VP64UTRN Pro...nger 1)1,4201,4401,4601,4801,5001,5201,5401,560
    gatgcactggatgactttgacctggacatgctcggatcataggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctacgtgacctactgaaactggacctgtacgagcctagtatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtaApaIAvaIBclIPmeITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMIEcoO109IDA274LD276DF278DL280DM282LG284S760-1615*1VP64c-myc epitopeBGH polyaden...on sequencePolyhi...e tag1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtBbsIBGH polyadenylation sequence1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacaacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtClaISphIBspDIPvuIIBGH pA1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagagaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatctNaeINgoMIV2,1202,1402,1602,1802,2002,2202,2402,260
    agttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggtcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatccBarIMscIXbaIBsaXIBsmBIEsp3IPshAIHygromycinFRT2,2802,3002,3202,3402,3602,3802,4002,4202,440
    agggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgtcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacEcoRIDraIIIHygromycin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    caagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatggttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacNcoINdeIPvuIAsiSIBfuAIBspMIRsrIIBstAPIHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactgacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacBstAPIDraIIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    gagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagataAjuISacIIHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtScaIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttacgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatNaeIPfoINgoMIVHygromycinSV40 pA3,3403,3603,3803,4003,4203,4403,4603,4803,500
    taatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaBsmIPsiIBstZ17ISV40 pA3,5203,5403,5603,5803,6003,6203,6403,660
    tgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagagacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctcPvuII3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttPciISapIBsaXIBspQIpUC ori3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggpUC ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    taagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccAcuIAlwNIpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtBspHIpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataattttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattAhdIAmp (bla)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgAmp (bla)4,9204,9404,9604,9805,0005,0205,0405,060
    tagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaAclIFspIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcaBcgIPvuIScaIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    caatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagaccAclIAcuIXmnIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcSspIBspHIbla promoterAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    cgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgAlwNISgrDI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWIBpu10I5,9605,9806,0006,020
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6034 bp45005000550050010001500VP64CMVTetO760-1615EW112.2 (DNA)FrtF (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)

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    6034