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EK0329 CMV-TO-FRB-MCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcBmrINdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgggatccctggagatgtggcatgaaggcctggaagaggcatctcgtttgtactttggggaaaggaacgtgaaaggcatgtttgaggtgctggagcccttgcatgctatgatggattctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtaccctagggacctctacaccgtacttccggaccttctccgtagagcaaacatgaaacccctttccttgcactttccgtacaaactccacgacctcgggaacgtacgatactacctBbsIPmeISphIStuIXcmIAflIIBamHIEcoNIHindIIIM1G2S3760-768L1E2M3W4H5E6G7L8E9E10A11S12R13L14Y15F16G17E18R19N20V21K22G23M24F25E26V27L28E29P30L31H32A33M34M35E36769-1044from AV134FRBCMV promot...th TetOs)720740760780800820840860
    acggGGACCCcagactctgaaggaaacatcctttaatcaggcctatggtcgagatttaatggaggcccaagagtggtgcaggaagtacatgaaatcagggaatgtcaaggacctcctccaagcctgggacctctattatcatgtgttccgacgaatctcaaagactagaggcgcctgccCCTGGGgtctgagacttcctttgtaggaaattagtccggataccagctctaaattacctccgggttctcaccacgtccttcatgtactttagtcccttacagttcctggaggaggttcggaccctggagataatagtacacaaggctgcttagagtttctgatctccgcggBsgIKasIKflINarISfoIStuIPluTIG1A2R37G38P39Q40T41L42K43E44T45S46F47N48Q49A50Y51G52R53D54L55M56E57A58Q59E60W61C62R63K64Y65M66K67S68G69N70V71K72D73L74L75Q76A77W78D79L80Y81Y82H83V84F85R86R87I88S89K90T91R92769-1044from AV134FRB8809009209409609801,0001,0201,040
    gggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAAccctgtcctcccagacccccaccgtgaccgcctagacctcctctgatgttcctgctactgctgttgccgcctagcccccccagaccaccatcaccgccaagcccccctccatggccaTACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTAgeIKpnIPsrIAcc65IG3T4G5G6S7G8G9G10T11G12G13S14G15G16D17Y18K19D20D21D22D23N24G25G26S27G28G29S30G31G32S33G34G35S36G37G38G39T40G411045-1167MA2SN4FT6QF8VL10VD12NG14GT16GD18V1168-189345-ECLfrom EK0006MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAXcmIT20VA22PS24NF26AN28GI30AE32WI34SS36NS38RS40QA42YK44VT46CS48VR50QS52SA54QN56RK58YT60IK62VE64VP66KG68AW70RS72YL74NM76EL781168-1893MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACAgeIAsiSIBstEIITI80PI82FA84TN86SD88CE90LI92VK94AM96QG98LL100KD102GN104PI106PS108AI110AA112NS114GI116YG118GG120TG122GG124SM126AS128NF130TQ132FV134LV1361168-1893from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPMCP1,4201,4401,4601,4801,5001,5201,5401,560
    GACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTABplIBplIBstXIBstZ17IDN138GG140TG142DV144TV146AP148SN150FA152NG154IA156EW158IS160SN162SR164SQ166AY168KV170TC172SV174RQ176SS178AQ180NR182KY184TI186KV188EV190PK192GA1941168-1893from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP1,5801,6001,6201,6401,6601,6801,7001,7201,740
    GGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGAATTTACtgagccgctcgaggagggcccgaacaaaaactCCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGCCTTAAATGactcggcgagctcctcccgggcttgtttttgaApaIAvaITliIXhoIBsoBIPspXIPaeR7IPspOMI*1WR196SY198LN200ME202LT204IP206IF208AT210NS212DC214EL216IV218KA220MQ222GL224LK226DG228NP230IP232SA234IA236AN238SG240IY2421168-1893from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPc-...e1,7601,7801,8001,8201,8401,8601,8801,9001,920
    catctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattBclIPmeIH1H2H3H4H5H61960-1977BGH polyadenylation sequencec-myc epitopePolyhi...e tag1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagacBbsIClaISphIBspDIBGH polyadenylation sequenceBGH pA2,1202,1402,1602,1802,2002,2202,2402,260
    aggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttcctccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggNaeIPvuIINgoMIV2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcBarIKflIMscIXbaIFRTHygromycin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcagctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtPflFIPshAITth111IHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ctttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgcPstIAsiSIBfuAIBspMIEcoRIDraIIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    gccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgNdeIPflFIRsrIITth111IHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacAjuIDraIIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggSacIIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggtcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaScaIHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    tgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacBsmINaeIPfoIPsiINgoMIVSV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgaaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactBstZ17ISV40 pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggccactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgSapIBspQIPvuII4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcPciIAflIIIpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagtgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcapUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcggatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcpUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaagtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcattBspHIpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    acttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctctgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgagAhdIBmrIBsaIBsrDIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcAclIFspIBsrDIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaaAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    actgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggctgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgAclIBcgIScaIXmnIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    gaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtaAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagSspIBspHISgrDIbla promoter5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccMfeIBsiWIBpu10I6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ccagatatacgcggtctatatgcgMluIAflIII6,310
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6312 bp450050005500600050010001500MCPRapalog MutTetO760-7681045-11671168-1893769-1044EWo100.1 (DNA)EKo237.1 (DNA)EKo271.3 (DNA)EKo309.1 (DNA)EKo237.2 (DNA)EKo309.2 (DNA)EKo329.1 (DNA)EK-ECLF (DNA)EKo329.3 (DNA)EKo329.5 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)EKo152.4 (DNA)EKo272.1 (DNA)EK_CMVFa (DNA),EK_CMVFb (DNA),SpeICMV,CMV promoter (with TetOs),EK_CMVFc (DNA),EK_CMVFd (DNA),NdeI,BmrI,+1A,EK_CMVFe (DNA),CspCITATA box,CMV-Forward Genewiz (DNA),Eco53kI,SacITetO,TetR binding sites,SgrDI,Eco53kI,SacICMVF1 (DNA),ToFlpF (DNA),BbsI,PmeIKozak,EKbF (DNA),EKo272.2 (DNA),+8SphIfrom AV134,FRB,KflI,StuIBsgIEKo329.2 (DNA)EKo35.2 (DNA),KasI,NarI,SfoI,PluTI45-ECL,from EK0006Acc65IAgeIKpnI,PsrIMCPXcmIBstEIIAsiSIAgeI+1BspHIAhdIBmrIBsaIBsrDIFspIAclI,BsrDIAmp (bla)ScaIBcgIAclIXmnIbla promoter,oLSM0054 (DNA),SspI,BspHIFlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo279.1 (DNA),SgrDI,MfeI,Bpu10I,BsiWI,+2

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    6312

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