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  • 0 feature results
XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export
  • Sequence Map
    Editing disabled on read-only sequence.
    tccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatZraIAatIISgrDI20406080100120140160
    caacaaggcaaggcttgaccgacaattgcatgaagaatcGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTgttgttccgttccgaactggctgttaacgtacttcttagCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAgeIMfeIMfeIEF1Alpha180200220240260280300320340
    TTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGGACCGACGTCBsgIXmnIEF1Alpha360380400420440460480500520
    TACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGStuIStuIAflIIEF1Alpha540560580600620640660680700
    TAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGApaIBsgIPciIPspOMIEF1Alpha720740760780800820840860
    ACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCFseIPasISacIEco53kIEF1Alpha8809009209409609801,0001,0201,040
    CGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGcGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGAGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCgCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTKasINarISfoIBbvCIPluTIBpu10IEF1AlphaEF1Alpha1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGaagctACTAGTtcgcACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACttcgaTGATCAagcgSpeIEF1Alphahomolog1,2401,2601,2801,3001,3201,3401,3601,3801,400
    caccATGGCGGCCCCTGCTCTGTCCTGGCGTCTGCCCCTCCTCATCCTCCTCCTGCCCCTGGCTACCTCTTGGGCATCTGCAgactacaaggatgacgatgataagGGTACCGCGGTGAATGGCACTTCCCAGTTCACATGCTTCTACAATTCGAGAGCCAACATCTCCTGTGTCgtggTACCGCCGGGGACGAGACAGGACCGCAGACGGGGAGGAGTAGGAGGAGGACGGGGACCGATGGAGAACCCGTAGACGTctgatgttcctactgctactattcCCATGGCGCCACTTACCGTGAAGGGTCAAGTGTACGAAGATGTTAAGCTCTCGGTTGTAGAGGACACAGBaeIKpnINcoIAcc65IGT2AV4NG6TS8QF10TC12FY14NS16RA18NI20SC22V1507-2229D1Y2K3D4D5D6D7K81483-1506M1A2A3P4A5L6S7W8R9L10P11L12L13I14L15L16L17P18L19A20T21S22W23A24S25A261405-1482IL-2Rbeta Signal PeptidehIL-2Rbeta ectodomainFLAGhomologhomolog1,4201,4401,4601,4801,5001,5201,5401,560
    TGGAGCCAAGATGGGGCTCTGCAGGACACTTCCTGCCAAGTCCATGCCTGGCCGGACAGACGGCGGTGGAACCAAACCTGTGAGCTGCTCCCCGTGAGTCAAGCATCCTGGGCCTGCAACCTGATCCTCGGAGCCCCAGATTCTCAGAAACTGACCACAGTTGACATCGTCACCCACCTCGGTTCTACCCCGAGACGTCCTGTGAAGGACGGTTCAGGTACGGACCGGCCTGTCTGCCGCCACCTTGGTTTGGACACTCGACGAGGGGCACTCAGTTCGTAGGACCCGGACGTTGGACTAGGAGCCTCGGGGTCTAAGAGTCTTTGACTGGTGTCAACTGTAGCAGTGGGW24SQ26DG28AL30QD32TS34CQ36VH38AW40PD42RR44RW46NQ48TC50EL52LP54VS56QA58SW60AC62NL64IL66GA68PD70SQ72KL74TT76VD78IV80T1507-2229hIL-2Rbeta ectodomain1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGAGGGTGCTGTGCCGTGAGGGGGTGCGATGGAGGGTGATGGCCATCCAGGACTTCAAGCCCTTTGAGAACCTTCGCCTGATGGCCCCCATCTCCCTCCAAGTTGTCCACGTGGAGACCCACAGATGCAACATAAGCTGGGAAATCTCCCAAGCCTCCCACTACTTTGAAAGACAACTCCCACGACACGGCACTCCCCCACGCTACCTCCCACTACCGGTAGGTCCTGAAGTTCGGGAAACTCTTGGAAGCGGACTACCGGGGGTAGAGGGAGGTTCAACAGGTGCACCTCTGGGTGTCTACGTTGTATTCGACCCTTTAGAGGGTTCGGAGGGTGATGAAACTTTCTGTBsaIMscIPmlIBsaBIBsu36IL82RV84LC86RE88GV90RW92RV94MA96IQ98DF100KP102FE104NL106RL108MA110PI112SL114QV116VH118VE120TH122RC124NI126SW128EI130SQ132AS134HY136FE138RH1401507-2229hIL-2Rbeta ectodomain1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CCTGGAGTTCGAGGCCCGGACGCTGTCCCCAGGCCACACCTGGGAGGAGGCCCCCCTGCTGACTCTCAAGCAGAAGCAGGAATGGATCTGCCTGGAGACGCTCACCCCAGACACCCAGTATGAGTTTCAGGTGCGGGTCAAGCCTCTGCAAGGCGAGTTCACGACCTGGAGCCCCGGACCTCAAGCTCCGGGCCTGCGACAGGGGTCCGGTGTGGACCCTCCTCCGGGGGGACGACTGAGAGTTCGTCTTCGTCCTTACCTAGACGGACCTCTGCGAGTGGGGTCTGTGGGTCATACTCAAAGTCCACGCCCAGTTCGGAGACGTTCCGCTCAAGTGCTGGACCTCGGGGXcmIBsmBIEsp3ILE142FE144AR146TL148SP150GH152TW154EE156AP158LL160TL162KQ164KQ166EW168IC170LE172TL174TP176DT178QY180EF182QV184RV186KP188LQ190GE192FT194TW196SP1981507-2229hIL-2Rbeta ectodomain1,9401,9601,9802,0002,0202,0402,0602,0802,100
    TGGAGCCAGCCCCTGGCCTTCAGGACAAAGCCTGCAGCCCTTGGGAAGGACACCATTCCGTGGCTCGGCCACCTCCTCGTGGGCCTCAGCGGGGCTTTTGGCTTCATCATCTTAGTGTACTTGCTCATCGGTACCgcggccgccaagagcaggatcaccagcgagggcgagtacaACCTCGGTCGGGGACCGGAAGTCCTGTTTCGGACGTCGGGAACCCTTCCTGTGGTAAGGCACCGAGCCGGTGGAGGAGCACCCGGAGTCGCCCCGAAAACCGAAGTAGTAGAATCACATGAACGAGTAGCCATGGcgccggcggttctcgtcctagtggtcgctcccgctcatgtKpnINotIBbvCIPflMIPflMIAcc65IBpu10IG1T2WS200QP202LA204FR206TK208PA210AL212GK214DT216IP218WL220GH222LL224VG226LS228GA230FG232FI234IL236VY238LL240I1507-2229K1S2R3I4T5S6E7G8E9Y102245-2304A1A2A3hIL-2Rbeta TransmembranehIL-2Rbeta ectodomainTraffic signalhomologPCR2,1202,1402,1602,1802,2002,2202,2402,260
    tccccctggaccagatcgacatcaacgtgTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCagggggacctggtctagctgtagttgcacAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGBbsIBsaBIBstEIII11P12L13D14Q15I16D17I18N19V202245-2304LH2LD4QT6PS8RQ10PI12PS14EG16LQ18LH20LP22QV24LA26DA28VS30RL32VL34GK36FG38DL40TD42NF44SS46PH48A2305-3537hADAR2 cat domainTraffic signalPCR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGAAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTR50RK52VL54AG56VV58MT60TG62TD64VK66DA68KV70IS72VS74TG76TK78CI80NG82EY84MS86DR88GL90AL92ND94CH96AE98II100SR102RS104LL106R2305-3537hADAR2 cat domain2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGBclIBsrGIF108LY110TQ112LE114LY116LN118NK120DD122QK124RS126IF128QK130SE132RG134GF136RL138KE140NV142QF144HL146YI148ST150SP152CG154DA156RI158FS160PH162EP164I2305-3537hADAR2 cat domain2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCBcgIL166EE168PA170DR172HP174NR176KA178RG180QL182RT184KI186ES188GQ190GT192IP194VR196SN198AS200IQ202AW204DG206VL208QG210ER212LL214TM216SC218SD220KI222AR2242305-3537hADAR2 cat domainhomologousDim...ce2,8202,8402,8602,8802,9002,9202,9402,960
    CTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACBlpITliIXhoIBamHIPpuMIPaeR7IWN226VV228GI230QG232SL234LS236IF238VE240PI242YF244SS246II248LG250SL252YH254GD256HL258SR260AM262YQ264RI266SN268IE270DL272PP274LY276TL278NK280PL2822305-3537hADAR2 cat domainhomologhomolog2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACMluILS284GI286SN288AE290AR292QP294GK296AP298NF300SV302NW304TV306GD308SA310IE312VI314NA316TT318GK320DE322LG324RA326SR328LC330KH332AL334YC336RW338MR3402305-3537hADAR2 cat domain3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAVH342GK344VP346SH348LL350RS352KI354TK356PN358VY360HE362SK364LA366AK368EY370QA372AK374AR376LF378TA380FI382KA384GL386GA388WV390EK392PT394EQ396DQ398F2305-3537hADAR2 cat domain3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CTCACTCACGCCCttctgctacgagaacgaggtgtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttGAGTGAGTGCGGGaagacgatgctcttgctccacactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaaApaIBclIPmeITliIXhoIPspXIPaeR7IPspOMIS400LT402PF404CY406EN408EV410*2305-3537BGH polyadenylation sequencec-myc epitopeER exportPolyhi...e taghomologhAD...in3,5203,5403,5603,5803,6003,6203,6403,660
    gtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctgcaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacBbsISphIBGH polyadenylation sequence3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgcccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacgClaIBspDIBGH pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttccgtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggKflIPpuMIFRT4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcBarIMscIXbaIZraIAatIIBsmBIEsp3IPshAIHygromycinFRT4,2204,2404,2604,2804,3004,3204,3404,360
    tggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctgEcoRIHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcgacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagNcoINdeIPvuIAsiSIRsrIIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    actggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgatgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgAjuIHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    ttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagScaIHygromycin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccPsiISV40 pAHygromycin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttataatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataBsmIBstZ17ISV40 pA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggttggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaa5,6205,6405,6605,6805,7005,7205,7405,760
    tgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcattPciISapIBspQIpUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatactttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatgpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacgacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    acgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagpUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    ttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttccpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    atcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctAhdI*W286HK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI2566731-7591Amp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcaBsaIRS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT1986731-7591Amp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcttcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagFspIPvuILL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI1406731-7591Amp (bla)7,0207,0407,0607,0807,1007,1207,1407,160
    gttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaataccaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgBcgIScaITT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82R6731-7591Amp (bla)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgXmnIS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24A6731-7591Amp (bla)7,3607,3807,4007,4207,4407,4607,4807,5007,520
    aaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaSspIF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M6731-7591Amp (bla)bla promoter7,5407,5607,5807,6007,6207,6407,6607,680
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7690 bp550060006500700050010001500homolog1483-15061405-14821507-22296731-7591FlpInF (DNA)EKo273.1 (DNA)EKo152.4 (DNA)XZp229.2 (DNA)M13-48REV Genewiz (DNA)FlpInF1 (DNA),ZraI,SgrDI,AatII,MfeI,MfeI,AgeIBsgI,XmnIEF1Alpha,StuI,AflII,StuIBsgI,PspOMI,ApaI,PciIFseI,PasIEco53kI,SacIBbvCI,Bpu10I,KasI,NarI,SfoI,PluTIEF1AlphaEF1a-seq-F (DNA),IGF1R-seq-F0 (DNA)homolog,Kozak,XZp375.1 (DNA),SpeI,NcoIIL-2Rbeta Signal Peptide,homolog,FLAG,KpnI,+4BaeIBsu36IhIL-2Rbeta ectodomain,BsaBI,MscIBsaIPmlIBspQI,SapIpUCori5F (DNA)PciIpUCoriR (DNA)pUC oriAhdIEKo152.2 (DNA),BsaIFspIAmp (bla),PvuI,ScaI,BcgIbla promoter,XmnI,SspI

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    7690

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