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pLV-CS-060 Addgene
  • Sequence Map
    Editing disabled on read-only sequence.
    cgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgccttta20406080100120140160
    gttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcaactttgtttcaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagttgaaacaaaSalISspIBspHISgrDI180200220240260280300320340
    attgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaccccattaacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctagttgacctattgagttcgattggttttagtagggtttgaagggtggggtaBsmIPsiISV40-PA terminator360380400420440460480500520
    accctattaccactgccaattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgtatttgttaaatatgtactacaaacttagtagttggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctacctgggataatggtgacggttaatggacaccaaagtaaatgagatttggacactaaggagacttaataaaagtaaaatttctttaacataaacaatttatacatgatgtttgaatcatcaaccttcccgattaagtgagggtttcttctgttctataggaactagacacctagatgg540560580600620640660680700
    acacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgtgtgttccgatgaagggactaatcgtcttgatgtgtggtcccggtccccagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcggtctattccatcttctccggttatttcctctcttgtggtcgaacaatgtgggacactcggacgtaccctacctDraIII720740760780800820840860
    tgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactggggagtggactgggcctctctcttcacaatctcacctccaaactgtcggcggatcgtaaagtagtgcaccgggctctcgacgtaggcctcatgaagttcttgacgactatagctcgaacgatgttccctgaaaggcgacccctgaaaggtccctccgcaccggacccgccctgacccctcaccPmlIScaIBsaAI8809009209409609801,0001,0201,040
    cgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctgctcgggagtctaggacgtatattcgtcgacgaaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaAflII5' LTR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttBssHIIPsi5' LTR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaNruIGAGPsi1,4201,4401,4601,4801,5001,5201,5401,560
    cctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccSapIBspQIGAG1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagattctcgttttgttttcattctggtggcgtgtcgttcgccggccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctFseIMfeIEcoNIGAG1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgccttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgBbvCIPRE1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcattgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtBsmIPREENV2,1202,1402,1602,1802,2002,2202,2402,260
    cacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaagtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttENV2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ttattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacaaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctctgtKflIENV2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gagacagatccattcgattagtgaacggatctcgacggtatcgcctttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacactctgtctaggtaagctaatcacttgcctagagctgccatagcggaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtCPPTENV2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gggacagcagagatccagtttatcgatttaaatttacttgtacagctcgtccatgccgccggtggagtggcggccctcggcgcgttcgtactgttccacgatggtgtagtcctcgttgtgggaggtgatgtccaacttgatgttgacgttgtaggcgccgggcagctgcacgggcccctgtcgtctctaggtcaaatagctaaatttaaatgaacatgtcgagcaggtacggcggccacctcaccgccgggagccgcgcaagcatgacaaggtgctaccacatcaggagcaacaccctccactacaggttgaactacaactgcaacatccgcggcccgtcgacgtgcccgClaISwaIBspDISgrAImCherry2,8202,8402,8602,8802,9002,9202,9402,960
    ttcttggccttgtaggtggtcttgacctcagcgtcgtagtggccgccgtccttcagcttcagcctctgcttgatctcgcccttcagggcgccgtcctcggggtacatccgctcggaggaggcctcccagcccatggtcttcttctgcattacggggccgtcggaggggaagttggaagaaccggaacatccaccagaactggagtcgcagcatcaccggcggcaggaagtcgaagtcggagacgaactagagcgggaagtcccgcggcaggagccccatgtaggcgagcctcctccggagggtcgggtaccagaagaagacgtaatgccccggcagcctccccttcaaccBbvCImCherry2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgccgcgcagcttcaccttgtagatgaactcgccgtcctgcagggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcaagtagtcggggatgtcggcggggtgcttcacgtaggccttggaacggcgcgtcgaagtggaacatctacttgagcggcaggacgtccctcctcaggacccagtgccagtggtgcggcggcaggagcttcaagtagtgcgcgagggtgaacttcgggagccccttcctgtcgaagttcatcagcccctacagccgccccacgaagtgcatccggaacctAloISbfIBsaAImCherry3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gccgtacatgaactgaggggacaggatgtcccaggcgaagggcagggggccacccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgtgcaccttgaagcgcatgaaccggcatgtacttgactcccctgtcctacagggtccgcttcccgtcccccggtgggaaccagtggaagtcgaaccgccagacccacgggagcatccccgccgggagcgggagcgggagctagagcttgagcaccggcaagtgcctcgggaggtacacgtggaacttcgcgtacttgAhdImCherry3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tccttgatgatggccatgttatcctcctcgcccttgctcaccatggtggcgttaacgcgatctgacggttcactaaacgagctctgcttatataggcctcccaccgtacacgcctacctactgactgcagtctgagtctgacagcctctagagtgttgtaaagctagcggagcagaggaactactaccggtacaataggaggagcgggaacgagtggtaccaccgcaattgcgctagactgccaagtgatttgctcgagacgaatatatccggagggtggcatgtgcggatggatgactgacgtcagactcagactgtcggagatctcacaacatttcgatcgcctcgtcBmtIHpaINheIXbaIPshAImCherryBarcode ...ng site3,5203,5403,5603,5803,6003,6203,6403,660
    agctacgcactctacgctagaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcttcgaagccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaatcgatgcgtgagatgcgatctccatccgcacatgccaccctccggatatattcgtctcgagcaaatcacttggcagtctagcgaagcttcggtggtaccactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtgttBstBIEGFPminCMVp3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttccaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggactggatgccgcacgtcacgaagtcggcgatggggctggtgtacttcgtcgtgctgaagaagEGFP3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgtgttcgacctcaEGFP4,0404,0604,0804,1004,1204,1404,1604,1804,200
    acaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactaccttgttgatgttgtcggtgttgcagatatagtaccggctgttcgtcttcttgccgtagttccacttgaagttctaggcggtgttgtagctcctgccgtcgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctgttggtgatggaEGFP4,2204,2404,2604,2804,3004,3204,3404,360
    gagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaattaattaactaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagctcgtgggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtacctgctcgacatgttcattaattaattgatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtctcPacIEGFPEF1a-core (SAM v2)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgatccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaactaggccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaMfeIEcoNIEF1a-core (SAM v2)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttcgcaacgggtttgccgccagaacacagggaattcatggcttcaaactttactcagttcgtgctcgtggacaatggtgggacaggggatgtgacagtggctccttctaatttcgctaatggggtggcagagtggatcagctccaactcacggagccaggcctacaaggtgacataagcgttgcccaaacggcggtcttgtgtcccttaagtaccgaagtttgaaatgagtcaagcacgagcacctgttaccaccctgtcccctacactgtcaccgaggaagattaaagcgattaccccaccgtctcacctagtcgaggttgagtgcctcggtccggatgttccactgtaEcoRIMCPEF1a-core (SAM v2)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcagcgtcaggcagtctagtgcccagaagagaaagtataccatcaaggtggaggtccccaaagtggctacccagacagtgggcggagtcgaactgcctgtcgccgcttggaggtcctacctgaacatggagctcactatcccaattttcgctaccaattctgactgtgaactcatcgtcgcagtccgtcagatcacgggtcttctctttcatatggtagttccacctccaggggtttcaccgatgggtctgtcacccgcctcagcttgacggacagcggcgaacctccaggatggacttgtacctcgagtgatagggttaaaagcgatggttaagactgacacttgagtaNspIBstXIBstZ17IMCP4,9204,9404,9604,9805,0005,0205,0405,060
    cgtgaaggcaatgcaggggctcctcaaagacggtaatcctatcccttccgccatcgccgctaactcaggtatctacagcgctggaggaggtggaagcggaggaggaggaagcggaggaggaggtagcggacctaagaaaaagaggaaggtggcggccgctggatccccttcaggggcacttccgttacgtccccgaggagtttctgccattaggatagggaaggcggtagcggcgattgagtccatagatgtcgcgacctcctccaccttcgcctcctcctccttcgcctcctcctccatcgcctggattctttttctccttccaccgccggcgacctaggggaagtcccAfeIBaeINotIBamHIMCPLinker (GGS)5NLSp655,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagatcagcaaccaggccctggctctggcccctagctccgctccagtgctggcccagactatggtgccctctagtgctatggtgcctctggcccagccacctgctccagcccctgtgctgaccccaggaccaccccagtcactgagcgctccagtgcccaagtctacacaggccggtctagtcgttggtccgggaccgagaccggggatcgaggcgaggtcacgaccgggtctgataccacgggagatcacgataccacggagaccgggtcggtggacgaggtcggggacacgactggggtcctggtggggtcagtgactcgcgaggtcacgggttcagatgtgtccggcAfeIPaqCIp655,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcgaggggactctgagtgaagctctgctgcacctgcagttcgacgctgatgaggacctgggagctctgctggggaacagcaccgatcccggagtgttcacagatctggcctccgtggacaactctgagtttcagcagctgctgaatcagggcgtgtccatgtctcatagtacagccgctcccctgagactcacttcgagacgacgtggacgtcaagctgcgactactcctggaccctcgagacgaccccttgtcgtggctagggcctcacaagtgtctagaccggaggcacctgttgagactcaaagtcgtcgacgacttagtcccgcacaggtacagagtatcatgtcgPaqCIp655,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgaaccaatgctgatggagtaccccgaagccattacccggctggtgaccggcagccagcggccccccgaccccgctccaactcccctgggaaccagcggcctgcctaatgggctgtccggagatgaagacttctcaagcatcgctgatatggactttagtgccctgctgtcacaggcttggttacgactacctcatggggcttcggtaatgggccgaccactggccgtcggtcgccggggggctggggcgaggttgaggggacccttggtcgccggacggattacccgacaggcctctacttctgaagagttcgtagcgactatacctgaaatcacgggacgacagtgtcPasIp655,6205,6405,6605,6805,7005,7205,7405,760
    atttcctctagtgggcagggaggaggtggaagcggcttcagcgtggacaccagtgccctgctggacctgttcagcccctcggtgaccgtgcccgacatgagcctgcctgaccttgacagcagcctggccagtatccaagagctcctgtctccccaggagccccccaggcctcccgtaaaggagatcacccgtccctcctccaccttcgccgaagtcgcacctgtggtcacgggacgacctggacaagtcggggagccactggcacgggctgtactcggacggactggaactgtcgtcggaccggtcataggttctcgaggacagaggggtcctcggggggtccggagggcHSF1p65GGGGS5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aggcagagaacagcagcccggattcagggaagcagctggtgcactacacagcgcagccgctgttcctgctggaccccggctccgtggacaccgggagcaacgacctgccggtgctgtttgagctgggagagggctcctacttctccgaaggggacggcttcgccgaggaccccactccgtctcttgtcgtcgggcctaagtcccttcgtcgaccacgtgatgtgtcgcgtcggcgacaaggacgacctggggccgaggcacctgtggccctcgttgctggacggccacgacaaactcgaccctctcccgaggatgaagaggcttcccctgccgaagcggctcctggggtgHSF15,9605,9806,0006,0206,0406,0606,0806,1006,120
    catctccctgctgacaggctcggagcctcccaaagccaaggaccccactgtctcctgtacagcaacaaacttctctctgctgaaacaagccggagatgtcgaagagaatcctggaccgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtagtagagggacgactgtccgagcctcggagggtttcggttcctggggtgacagaggacatgtcgttgtttgaagagagacgactttgttcggcctctacagcttctcttaggacctggctggctcatgttcgggtgccacgcggagcggtgggcgctgctgcaggggtcccggcatPasIBsiWIPflFITth111ITE2YK4PT6VR8LA10TR12DD14VP16RA18VPuroRA1T2N3F4S5L6L7K8Q9A10G11D12V13E14E15N16P17G18P19P2AHSF16,1406,1606,1806,2006,2206,2406,2606,2806,300
    cgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgcgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctaggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgctgccgcggcgccaccgccagacctggtgcgRsrIISacIIR20TL22AA24AF26AD28YP30AT32RH34TV36DP38DR40HI42ER44VT46EL48QE50LF52LT54RV56GL58DI60GK62VW64VA66DD68GA70AV72AV74WT76TPuroR6,3206,3406,3606,3806,4006,4206,4406,460
    cggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggagtctcgcccgaccaccagggcaagcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtggcagcctcagagcgggctggtggtcccgttP78ES80VE82AG84AV86FA88EI90GP92RM94AE96LS98GS100RL102AA104QQ106QM108EG110LL112AP114HR116PK118EP120AW122FL124AT126VG128VS130PD132HQ134GK136PuroR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgccccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacgggcttcctggcgcgtggaccacgtactgggcgSexAIBssHIIDraIIIGL138GS140AV142VL144PG146VE148AA150ER152AG154VP156AF158LE160TS162AP164RN166LP168FY170ER172LG174FT176VT178AD180VE182VP184EG186PR188TW190CM192TR194PuroR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    aagcccggtgcctgacgcgtctggaacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtatattcgggccacggactgcgcagaccttgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatMluIAflIIIKP196GA198*PuroRWPRE6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgcttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcgWPRE7,0207,0407,0607,0807,1007,1207,1407,160
    cgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttcctgcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagccccttcgactgcaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaWPRE7,1807,2007,2207,2407,2607,2807,3007,3207,340
    tcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctggaattaattctgcagtcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcggaccttaattaagacgtcagctctggatctttttgtacctcgttagtgttcatcgttatgtcgtcgatggttacgactaacacgSacIIWPRE7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaagaggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgaccgatcttcgtgttctcctcctcctccacccaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttctcccctgaccttcccgattaagtgagggttgcttctgttctagacgaaaaacgaacatgaKpnIAcc65IBsu36I7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctcccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagaAflII3' LTR7,7207,7407,7607,7807,8007,8207,8407,860
    ctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggccttgacattctagcgttttaccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaatgatcgtcatcatcaagtacagtagaataataagtcataaatattgaacgtttctttacttatagtctctcactctccggaactgtaagatcgcaaaatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaPsiISalIM13-revLacO7,8807,9007,9207,9407,9607,9808,0008,0208,040
    tccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataaccclac promoter8,0608,0808,1008,1208,1408,1608,1808,2008,220
    cgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgtgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaNspIPciISapIBspQIAflIII8,2408,2608,2808,3008,3208,3408,3608,3808,400
    tgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgccttacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaColE1 origin8,4208,4408,4608,4808,5008,5208,5408,560
    tctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcColE1 origin8,5808,6008,6208,6408,6608,6808,7008,7208,740
    ccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtColE1 origin8,7608,7808,8008,8208,8408,8608,8808,9008,920
    aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctattgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatBspHIColE1 origin8,9408,9608,9809,0009,0209,0409,0609,0809,100
    gatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgAhdIW220HK218IL216SA214GI212EA210IQ208RN206RE204DM202TA200QS198GT196TY194IV192VI190RS188PAmpR9,1209,1409,1609,1809,2009,2209,2409,260
    ttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgcaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcgK186GD184PG182LA180AI178IG176RS174GR172EG170AG168SK166DA164IF162WG160AP158LA156SR154LL152PG150AV148KD146AE144MW142DI140LQ138QR136SA134LT132LL130EG128AmpR9,2809,3009,3209,3409,3609,3809,4009,4209,440
    cagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaaggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcFspIPvuITL126LK124RL122TT120AM118AV116PM114TT112DR110ED108NP106IA104EN102LE100PE98WR96DL94RT92VH90DG88MN86HL84FA82TL80EK78PG76GI74TT72LL70AmpR9,4609,4809,5009,5209,5409,5609,5809,6009,620
    taagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggScaILN68AA66TN64DS62MT60IA58AS56CL54ER52VT50MG48DT46LH44KE42TV40PS38YE36VL34DN32QS30YH28IR26RG24LQ22EQ20GA18DI16RS14LV12AmpR9,6409,6609,6809,7009,7209,7409,7609,7809,800
    gcgccacatagcagaactttaaaagtgctcatcattggaaaacgcggtgtatcgtcttgaaattttcacgagtagtaaccttttAG10CL8LV6KF4TS2MAmpR9,8209,840
    • Plasmid
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    Editing disabled on read-only sequence.
    9842 bp700075008000850090009500500100015002000ENVGAGAmpR

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    9842