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  • 0 feature results
(pCas9 = pT698) p41-hphMX-Leu2-TEF1p-Cas9-CYC1t
  • Sequence Map
    Editing disabled on read-only sequence.
    GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCGGACCTCTGTACCTCCGGGTCTTATGGGAGGAACTGTCAGAACTGCACGCGTCGAGTCCCCGTACTACACTGACAGCGGGCATGTAAATCGGGTATGTAGGGGTACATATTAGTAAACGTAGGTATGTAAAACTACCGGCGTGCCGCGCTTCGTTTTTAATGCCGAGGAGCGACGCCTGGABmgBIAshbya gossypii TEF1 promoterHygMX20406080100120140160
    GCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACCATGGGTCGCTCGTCCCTTTGCGAGGGGAGTGTCTGCGCAACTTAACAGGGGTGCGGCGCGGGGACATCTCTTTATATTTTCCAATCCTAAACGGTGACTCCAAGAAGAAAGTATATGAAGGAAAATTTTAGAACGATCCTATGTCAAGAGTGTAGTGTAGGCTTGTATTTGTTGGTACCCAMluINcoIHygMXAshbya gossypii TEF1 promoter180200220240260280300320340
    AAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGTTTTTCGGACTTGAGTGGCGCTGCAGACAGCTCTTCAAAGACTAGCTTTTCAAGCTGTCGCAGAGGCTGGACTACGTCGAGAGCCTCCCGCTTCTTAGAGCACGAAAGTCGAAGCTACATCCTCCCGCACCTATACAGGACGCCCATTTATCGACGCGGCTACCAAAGATGTTTCZraIAatIIPshAIHygMXhphMX open reading frame (ORF)360380400420440460480500520
    ATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCTAGCAATACAAATAGCCGTGAAACGTAGCCGGCGCGAGGGCTAAGGCCTTCACGAACTGTAACCCCTTAAGTCGCTCTCGGACTGGATAACGTAGAGGGCGGCACGTGTCCCACAGTGCAACGTTCTGGACGGACTTTGGCTTGACGGGCGACAAGACGTCGGCCAGCGCCTCCGBstAPIHygMXhphMX open reading frame (ORF)540560580600620640660680700
    CATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGTACCTACGCTAGCGACGCCGGCTAGAATCGGTCTGCTCGCCCAAGCCGGGTAAGCCTGGCGTTCCTTAGCCAGTTATGTGATGTACCGCACTAAAGTATACGCGCTAACGACTAGGGGTACACATAGTGACCGTTTGACACTACCTGCTGTGGCAGTCACGCAGGCAGCGCGTCNcoINdeIAsiSIRsrIIHygMXhphMX open reading frame (ORF)720740760780800820840860
    GCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTCGAGAGCTACTCGACTACGAAACCCGGCTCCTGACGGGGCTTCAGGCCGTGGAGCACGTGCGCCTAAAGCCGAGGTTGTTACAGGACTGCCTGTTACCGGCGTATTGTCGCCAGTAACTGACCTCGCTCCGCTACAAGCCCCTAAGGGTTATGCTCCAGCGGTTGTAGAAGAAGABstAPIHygMXhphMX open reading frame (ORF)8809009209409609801,0001,0201,040
    GGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCCTCCGGCACCAACCGAACATACCTCGTCGTCTGCGCGATGAAGCTCGCCTCCGTAGGCCTCGAACGTCCTAGCGGCGCCGAGGCCCGCATATACGAGGCGTAACCAGAACTGGTTGAGATAGTCTCGAACCAACTGCCGTTAAAGCTACTACGTCGAACCCGCGTCCCAGCTACSacIIHygMXhphMX open reading frame (ORF)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAATCAGTACTGACAATAAAAAGATTCTTGGCTGCGTTAGCAGGCTAGGCCTCGGCCCTGACAGCCCGCATGTGTTTAGCGGGCGTCTTCGCGCCGGCAGACCTGGCTACCGACACATCTTCATGAGCGGCTATCACCTTTGGCTGCGGGGTCGTGAGCAGGCTCCCGTTTCCTTATTAGTCATGACTGTTATTTTTCTAAGAACHygMXhphMX open reading frame (ORF)Ashbya gos...erminator1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTAAAAGTTCTTGAACAGTAAACATATCAAAAAAATATAACATCAACAAGATAAAATTAGTTTACAATCGCACTAAATATAAAAAAAAGCGGAGCTGTAGTAGACGGGTCTACGCTTCAATTCACGCGTCTTTCATTATAGTACGCAGTTAGCATACACTTACGACCAGCGATATGAHygMXAshbya gossypii TEF1 terminator1,4201,4401,4601,4801,5001,5201,5401,560
    GCTGTCGATTCGATACTAACGCCGCCATCCAGTGTtaattaaCGACGGTCGAGGAGAACTTCTAGTATATCtACATACCTAATATTATTGCCTTATTAAAAATGGAATCCCAACAATTACATCAAAATCCACATTCTCTTCAAAATCAATTGTCCTGTACTTCCTTGTTCATGTGCGACAGCTAAGCTATGATTGCGGCGGTAGGTCACAattaattGCTGCCAGCTCCTCTTGAAGATCATATAGaTGTATGGATTATAATAACGGAATAATTTTTACCTTAGGGTTGTTAATGTAGTTTTAGGTGTAAGAGAAGTTTTAGTTAACAGGACATGAAGGAACAAGTACACPacIHygMXAshbya gossyp...1 terminator1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGTTCAAAAACGTTATATTTATAGGATAATTATACTCTATTTCTCAACAAGTAATTGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGAAATATCTTGACCGCAGTTAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTACAAGTTTTTGCAATATAAATATCCTATTAATATGAGATAAAGAGTTGTTCATTAACCAACAAACCGGCTCGCCAGATTCCGCGGACTAAGTTCTTTATAGAACTGGCGTCAATTGACACCCTTATGAGTCCATAGCATTCTACGTTCTCAAGCTTAGAGAATCGTTGGTAATAABaeIHpaILEU2 promoter1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGcGCCGGAACCGGCTTTTCATATAGAATAGAAAAAGGAGTTGTATTGCTCTTGTGTGTCCCCGCGATAGCGTGTCTTAGTTTAAGCTACTGACCTTTAAAAAACAATTAAAGTCTCCAGCGGACTGCGTATATGGAAAAAGTTGACTTTTTAACCCTCTTTTTCCTTTCCACTCTCgCGGCCTTGGCCGAAAAGTATATCTTATCLEU2 promoter1,9401,9601,9802,0002,0202,0402,0602,0802,100
    AGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAatATATATATATATATTTCAAGGATATACCATTCTAATGTTCTTCGCAAGTACTGATTTACGAACGTAGTGTTATGAACTTCAACTGTTATAATAAATTCCTGGATAACAAAAAAGGTTATCCACCAATCGTTAGCAGAATGAAAGATTGAAAAGAATGGAAAATGTAAAGTCGTtaTATATATATATATAAAGTTCCTATATGGTAAGATTACAMLEU2 promoter2,1202,1402,1602,1802,2002,2202,2402,260
    CTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTCGTTCCAATGTCAAGTTCGATTTCGAAAATCATTTAATTGGTGGTGCTGCTATCGATGCTACAGGTGTtCCACTTCCAGAGACGGGGATTCTTCTAGCAGCAAAACGGTCCACTGGTGCAACCAGTTCTTTAGTGTCGGCTTCGGTAATTCCAAGAATTTCGATAAAGACTACAAGCAAGGTTACAGTTCAAGCTAAAGCTTTTAGTAAATTAACCACCACGACGATAGCTACGATGTCCACAaGGTGAAGGTCTClaIBspDIBstEIIS2AP4KK6IV8VL10PG12DH14VG16QE18IT20AE22AI24KV26LK28AI30SD32VR34SN36VK38FD40FE42NH44LI46GG48AA50ID52AT54GV56PL58PD602272-3366LEU2 open reading frame (ORF)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TGAGGCGCTGGAAGCCTCCAAGAAGGTTGATGCCGTTTTGTTAGGTGCTGTGGGTGGTCCTAAATGGGGTACCGGTAGTGTTAGACCTGAACAAGGTTTACTAAAAATCCGTAAAGAACTTCAATTGTACGCCAACTTAAGACCATGTAACTTTGCATCCGACTCTCTTTTAGACACTCCGCGACCTTCGGAGGTTCTTCCAACTACGGCAAAACAATCCACGACACCCACCAGGATTTACCCCATGGCCATCACAATCTGGACTTGTTCCAAATGATTTTTAGGCATTTCTTGAAGTTAACATGCGGTTGAATTCTGGTACATTGAAACGTAGGCTGAGAGAAAATCTGAgeIKpnIPsrIAcc65IEA62LE64AS66KK68VD70AV72LL74GA76VG78GP80KW82GT84GS86VR88PE90QG92LL94KI96RK98EL100QL102YA104NL106RP108CN110FA112SD114SL116LD1182272-3366LEU2 open reading frame (ORF)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTATCTCCAATCAAGCCACAATTTGCTAAAGGTACTGACTTCGTTGTTGTCAGAGAATTAGTGGGAGGTATTTACTTTGGTAAGAGAAAGGAAGACGATGGTGATGGTGTCGCTTGGGATAGTGAACAATACACCGTTCCAGAAGTGCAAAGAATCACAAGAATGGCCGCTTTCAAATAGAGGTTAGTTCGGTGTTAAACGATTTCCATGACTGAAGCAACAACAGTCTCTTAATCACCCTCCATAAATGAAACCATTCTCTTTCCTTCTGCTACCACTACCACAGCGAACCCTATCACTTGTTATGTGGCAAGGTCTTCACGTTTCTTAGTGTTCTTACCGGCGAAAGTLS120PI122KP124QF126AK128GT130DF132VV134VR136EL138VG140GI142YF144GK146RK148ED150DG152DG154VA156WD158SE160QY162TV164PE166VQ168RI170TR172MA174AF1762272-3366LEU2 open reading frame (ORF)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TGGCCCTACAACATGAGCCACCATTGCCTATTTGGTCCTTGGATAAAGCTAATGTTTTGGCCTCTTCAAGATTATGGAGAAAAACTGTGGAGGAAACCATCAAGAACGAATTCCCTACATTGAAGGTTCAACATCAATTGATTGATTCTGCCGCCATGATCCTAGTTAAGAACCCACCGGGATGTTGTACTCGGTGGTAACGGATAAACCAGGAACCTATTTCGATTACAAAACCGGAGAAGTTCTAATACCTCTTTTTGACACCTCCTTTGGTAGTTCTTGCTTAAGGGATGTAACTTCCAAGTTGTAGTTAACTAACTAAGACGGCGGTACTAGGATCAATTCTTGGGXcmIMA178LQ180HE182PP184LP186IW188SL190DK192AN194VL196AS198SR200LW202RK204TV206EE208TI210KN212EF214PT216LK218VQ220HQ222LI224DS226AA228MI230LV232KN234P2272-3366LEU2 open reading frame (ORF)2,8202,8402,8602,8802,9002,9202,9402,960
    AACCCACCTAAATGGTATTATAATCACCAGCAAtATGTTTGGTGATATCATCTCCGATGAAGCCTCCGTTATCCCAGGTTCCTTGGGTTTGTTGCCATCTGCGTCCTTGGCCTCTTTGCCAGACAAGAACACCGCATTTGGTTTGTACGAACCATGCCACGGTTCTGCTCCAGATTTGGGTGGATTTACCATAATATTAGTGGTCGTTaTACAAACCACTATAGTAGAGGCTACTTCGGAGGCAATAGGGTCCAAGGAACCCAAACAACGGTAGACGCAGGAACCGGAGAAACGGTCTGTTCTTGTGGCGTAAACCAAACATGCTTGGTACGGTGCCAAGACGAGGTCTABstXIEcoRVT236HL238NG240II242IT244SN246MF248GD250II252SD254EA256SV258IP260GS262LG264LL266PS268AS270LA272SL274PD276KN278TA280FG282LY284EP286CH288GS290AP292D2272-3366LEU2 open reading frame (ORF)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TTGCCAAAGAATAAGGTTGACCCTATCGCCACTATCTTGTCTGCTGCAATGATGTTGAAATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGAACGGTTTCTTATTCCAACTGGGATAGCGGTGATAGAACAGACGACGTTACTACAACTTTAACAGTAACTTGAACGGACTTCTTCCATTCCGGTAACTTCTACGTCAATTTTTCCAAAACCTACGTCCATAGTCTTGACCACTAAATCCACCAAGGTTGTCATGGTGGCTTCAGCL294PK296NK298VD300PI302AT304IL306SA308AM310ML312KL314SL316NL318PE320EG322KA324IE326DA328VK330KV332LD334AG336IR338TG340DL342GG344SN346ST348TE350V2272-3366LEU2 open reading frame (ORF)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAAAAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATGAAATTCATAATAGAAACGACACGAAATTACAAAATGGAATATGTTCATAGGGTAGACGAAACTATATACGCAATCTACATACATTTATCACTACGACAGCGGCTTCTTCAATTCTTTTAGGAACGAATTTTTCTAAGAGAAAAAAATACTATAAACATGTATTTGAAATATTTACTTTAAGTATTATCTTTGCTGTGCTTTAATGTTTTACCTTATACAAGTATCCCATCTGCTTTGATATATGCGTTAGATGTATGTAAATABsrGIG352DA354VA356EE358VK360KI362LA364*2272-3366LEU2 terminatorLEU2 open reading frame (ORF)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CAAGAAGGAGAAAAAGGAGGATAGTAAAGGAATACAGGTAAGCAAATTGATACTAATGGCTCAACGTGATAAGGAAAAAGAATTGCACTTTAACATTAATATTGACAAGGAGGAGGGCACCACACAAAAAGTTAGGTGTAACAGAAAATCATGAAACTACGATTCCTAATTTGATGTTCTTCCTCTTTTTCCTCCTATCATTTCCTTATGTCCATTCGTTTAACTATGATTACCGAGTTGCACTATTCCTTTTTCTTAACGTGAAATTGTAATTATAACTGTTCCTCCTCCCGTGGTGTGTTTTTCAATCCACATTGTCTTTTAGTACTTTGATGCTAAGGATTAAACTALEU2 terminator3,5203,5403,5603,5803,6003,6203,6403,660
    ATTGGAGGATTTTCTCTAAAAAAAAAAAAATACAACAAATAAAAAACACTCAATGACCTGACCATTTGATGGAGTTTAAGTCAATACCTTCTTGAACCATTTCCCATAATGGTGAAAGTTCCCTCAAGAATTTTACTCTGTCAGAAACGGCCTTACGACGTAGTCGATATGGTGCTAACCTCCTAAAAGAGATTTTTTTTTTTTTATGTTGTTTATTTTTTGTGAGTTACTGGACTGGTAAACTACCTCAAATTCAGTTATGGAAGAACTTGGTAAAGGGTATTACCACTTTCAAGGGAGTTCTTAAAATGAGACAGTCTTTGCCGGAATGCTGCATCAGCTATACCACGLEU2 terminator3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGC3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGgATcgcttgcctgtaacttacacgcgcctcgtatcttttaatgatggaataatttgggaatttactctgtgtttatttatttttatgttttgtatttggatttGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCCTGCcTAgcgaacggacattgaatgtgcgcggagcatagaaaattactaccttattaaacccttaaatgagacacaaataaataaaaatacaaaacataaacctaaaCEN6_ARS44,0404,0604,0804,1004,1204,1404,1604,1804,200
    tagaaagtaaataaagaaggtagaagagttacggaatgaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaagcgtactttacatatatatttattagacaagaaaagcagattaaatagatatacattcgattaacgataagtaaaatgtaaaatcacaggatttatctttcatttatttcttccatcttctcaatgccttacttctttttttttatttgtttccaaattttttaaagttgtttttcgcatgaaatgtatatataaataatctgttcttttcgtctaatttatctatatgtaagctaattgctattcattttacattttagtgtcctaaaCEN6_ARS44,2204,2404,2604,2804,3004,3204,3404,360
    tcgtgtgtggtcttctacacagacaagatgaaacaattcggcattaatacctgagagcaggaagaAAaagataaaaggtagtatttgttggcgatccccctagagtcttttacatcttcggaaaacaaaaactattttttctttaatttctttttttactttctatttttaatttagcacacaccagaagatgtgtctgttctactttgttaagccgtaattatggactctcgtccttctTTttctattttccatcataaacaaccgctagggggatctcagaaaatgtagaagccttttgtttttgataaaaaagaaattaaagaaaaaaatgaaagataaaaattaaaCEN6_ARS44,3804,4004,4204,4404,4604,4804,5004,5204,540
    atatatttatattaaaaaatttaaattataattatttttatagcacgtgatgaaaaggacccaggtggcacttttcggggaaatgtgCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATtatataaatataattttttaaatttaatattaataaaaatatcgtgcactacttttcctgggtccaccgtgaaaagcccctttacacGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTAPmlISwaIAmpR promoterCEN6_ARS44,5604,5804,6004,6204,6404,6604,6804,7004,720
    ATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTAmpR open reading frame (ORF)AmpR ...oter4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAABcgIAmpR open reading frame (ORF)4,9204,9404,9604,9805,0005,0205,0405,060
    ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGTGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCAmpR open reading frame (ORF)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    CCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAABglIAmpR open reading frame (ORF)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    TATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCAhdIAmpR open reading frame (ORF)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    TAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCtcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaaAmpR terminatorColE1 originpBR322_origin5,6205,6405,6605,6805,7005,7205,7405,760
    gagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtColE1 originpBR322_origin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggaggaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcColE1 originpBR322_origin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagColE1 originpBR322_origin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgacggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactPciIColE1 originpBR322_origin6,3206,3406,3606,3806,4006,4206,4406,460
    gtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaaSapIBspQI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    aatgtgagttacctcactcattaggcaccccaggctttacactttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggAGCTCATAGttacactcaatggagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgaggatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtgatttcccttgttttcgaccTCGAGTATCSacIEco53kIM13-revT3T3 promoter6,6606,6806,7006,7206,7406,7606,7806,8006,820
    CTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTGAAGTTTTACAAAGATGAGGAAAAAATGAGAAGGTCTAAAAGAGCCTGAGGCGCGTAGCGGCATGGTGAAGTTTTGTGGGTTCGTGTCGTATGATTTAAAGGGGAGAAAGAAGGAGATCCCACAGCAATTAATGGGCATGATTTCCAAACCTTTTCTTTTTTCTCTGGCGGAGCATEF1 promoter6,8406,8606,8806,9006,9206,9406,9606,9807,000
    TTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTAAGAAAAAGAAGCAGCTTTTTCCGTTATTTTTAAAAATAGTGCAAAGAAAAAGAACTTTTAAAAAAAAAACTAAAAAAAGAGAAAGCTACTGGAGGGTAACTATAAATTCAATTATTTGCCAGAAGTTAAAGAGTTCAAAGTCAAAGTAAAAAGAACAAGATAATGTTGAAAAAATEF1 promoter7,0207,0407,0607,0807,1007,1207,1407,160
    TACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTCTAGAACTAGTGGATCCCCCGGGaaaaATGGACAAGAAGTACTCCATTGGGCTCGATATCGGCACAAACAGCGTCGGtTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGATGAAGAACGAGTAATCTTTCTTTCGTATCGTTAGATTAGATTCAAAAGATCTTGATCACCTAGGGGGCCCttttTACCTGTTCTTCATGAGGTAACCCGAGCTATAGCCGTGTTTGTCGCAGCCaACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCSmaISpeIXbaIXmaIBamHIEcoRVTspMIMD2KK4YS6IG8LD10IG12TN14SV16GW18AV20IT22DE24YK26VP28SK30KF32K7251-11391SpCas9TEF1 promoterkozak se...hancer)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TTCTGGGCAATACCGATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCCTGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAAAGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAAAGACCCGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGCCCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTTTCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTSbfIBglIIV34LG36NT38DR40HS42IK44KN46LI48GA50LL52FD54SG56ET58AE60AT62RL64KR66TA68RR70RY72TR74RK76NR78IC80YL82QE84IF86SN88EM90AK927251-11391SpCas97,3607,3807,4007,4207,4407,4607,4807,5007,520
    GGTGGATGACTCTTTCTTCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGGATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAACCATATATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACCCACCTACTGAGAAAGAAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGGTGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATATAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGXcmIVD94DS96FF98HR100LE102ES104FL106VE108ED110KK112HE114RH116PI118FG120NI122VD124EV126AY128HE130KY132PT134IY136HL138RK140KL142VD144ST146DK148AD1507251-11391SpCas97,5407,5607,5807,6007,6207,6407,6607,6807,700
    TTGCGGTTGATCTATCTCGCGCTGGCGCATATGATCAAATTTCGGGGACACTTCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTACAATCAGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAACGCCAACTAGATAGAGCGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGGGTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAAAGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTNdeISalILR152LI154YL156AL158AH160MI162KF164RG166HF168LI170EG172DL174NP176DN178SD180VD182KL184FI186QL188VQ190TY192NQ194LF196EE198NP200IN202AS204GV206DA2087251-11391SpCas97,7207,7407,7607,7807,8007,8207,8407,860
    AAGCAATCCTGAGCGCTAGGCTGTCCAAATCCCGGCGGCTCGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCAACTTTAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGTTCGTTAGGACTCGCGATCCGACAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGCCGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGATTGAAGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCPasIKA210IL212SA214RL216SK218SR220RL222EN224LI226AQ228LP230GE232KK234NG236LF238GN240LI242AL244SL246GL248TP250NF252KS254NF256DL258AE260DA262KL264QL266S7251-11391SpCas97,8807,9007,9207,9407,9607,9808,0008,0208,040
    CAAAGACACCTACGATGATGATCTCGACAATCTGCTGGCCCAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGAACACGGAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTATGTTTCTGTGGATGCTACTACTAGAGCTGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGGACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGTGGTTTCGAGGCGACTCGCGATCATACTAGTTCGCGATABlpIBsaBIK268DT270YD272DD274LD276NL278LA280QI282GD284QY286AD288LF290LA292AK294NL296SD298AI300LL302SD304IL306RV308NT310EI312TK314AP316LS318AS320MI322KR324Y7251-11391SpCas98,0608,0808,1008,1208,1408,1608,1808,2008,220
    GATGAGCACCACCAAGACTTGACTTTGCTGAAGGCCCTTGTCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGACGGCGGAGCAAGCCAGGAGGAATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATGGCTACTCGTGGTGGTTCTGAACTGAAACGACTTCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGATTTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCTTAAAATGTTTAAATAATTCGGGTAGAACCTTTTTTACCD326EH328HQ330DL332TL334LK336AL338VR340QQ342LP344EK346YK348EI350FF352DQ354SK356NG358YA360GY362ID364GG366AS368QE370EF372YK374FI376KP378IL380EK382M7251-11391SpCas98,2408,2608,2808,3008,3208,3408,3608,3808,400
    ACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAGAAGATCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTATCCTCAGGCGGCAAGAGGATTTCTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAGAATGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTCTTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGGACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAAGATGGGGAAAAACTTTCTATTGTCCCTTTTCTAACTCTTBsgIBglIIBsu36ID384GT386EE388LL390VK392LN394RE396DL398LR400KQ402RT404FD406NG408SI410PH412QI414HL416GE418LH420AI422LR424RQ426ED428FY430PF432LK434DN436RE438KI440EK4427251-11391SpCas98,4208,4408,4608,4808,5008,5208,5408,560
    AATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCGCCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGAAGTCGTGGATAAGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGACTAACTTTGATAAATTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGCGGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGACCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACTGATTGAAACTATTTSmaIXmaIBsaBITspMIIL444TF446RI448PY450YV452GP454LA456RG458NS460RF462AW464MT466RK468SE470ET472IT474PW476NF478EE480VV482DK484GA486SA488QS490FI492ER494MT496NF498DK5007251-11391SpCas98,5808,6008,6208,6408,6608,6808,7008,7208,740
    AATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCTGTACGAGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGCATTCCTGTCTGGAGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGAACCGGAAAGTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGACATGCTCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTTTCGGTCGTAAGGACAGACCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCTTGGCCTTTCBarISacIEco53kINL502PN504EK506VL508PK510HS512LL514YE516YF518TV520YN522EL524TK526VK528YV530TE532GM534RK536PA538FL540SG542EQ544KK546AI548VD550LL552FK554TN556RK5587251-11391SpCas98,7608,7808,8008,8208,8408,8608,8808,9008,920
    TTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTGAATGTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGATCTCCTGAAAATCATTAAAGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATTCTTGAAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAACTTACAAAGCTGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGCTAGAGGACTTTTAGTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAGAACTPasIVT560VK562QL564KE566DY568FK570KI572EC574FD576SV578EI580SG582VE584DR586FN588AS590LG592TY594HD596LL598KI600IK602DK604DF606LD608NE610EN612ED614IL616E7251-11391SpCas98,9408,9608,9809,0009,0209,0409,0609,0809,100
    GGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGATTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGATATACAGGATGGGGGCGGCTGTCAAGAAAACTGATCAATGGGATCCGAGACAAGCAGAGTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTAACTTCTTGCGAACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTATATGTCCTACCCCCGCCGACAGTTCTTTTGACTAGTTACCCTAGGCTCTGTTCGTCTCABamHICspCID618IV620LT622LT624LF626ED628RE630MI632EE634RL636KT638YA640HL642FD644DK646VM648KQ650LK652RR654RY656TG658WG660RL662SR664KL666IN668GI670RD672KQ674S7251-11391SpCas99,1209,1409,1609,1809,2009,2209,2409,260
    GGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCAACCGGAACTTCATGCAGTTGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCAGCTATCACCTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCAACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGGTCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTBcgIG676KT678IL680DF682LK684SD686GF688AN690RN692FM694QL696IH698DD700SL702TF704KE706DI708QK710AQ712VS714GQ716GD718SL720HE722HI724AN726LA728GS730PA732I7251-11391SpCas99,2809,3009,3209,3409,3609,3809,4009,4209,440
    AAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTCGTCAAAGTAATGGGAAGGCATAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAACTGGGTTTTCCCTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTATTCGGGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCTTGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCK734KG736IL738QT740VK742VV744DE746LV748KV750MG752RH754KP756EN758IV760IE762MA764RE766NQ768TT770QK772GQ774KN776SR778ER780MK782RI784EE786GI788KE790LG7927251-11391SpCas99,4609,4809,5009,5209,5409,5609,5809,6009,620
    GTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGCTTCAGAATGAGAAGCTCTACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACTACGACGTGGATCATATCGTGCCCCAGTCTTTTCTCAAAGATGATCAGGGTTTAGGAATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGGACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGATGCTGCACCTAGTATAGCACGGGGTCAGAAAAGAGTTTCTACTAKflIPciIBmgBISQ794IL796KE798HP800VE802NT804QL806QN808EK810LY812LY814YL816QN818GR820DM822YV824DQ826EL828DI830NR832LS834DY836DV838DH840IV842PQ844SF846LK848DD8507251-11391SpCas99,6409,6609,6809,7009,7209,7409,7609,7809,800
    TCTATTGATAATAAAGTGTTGACAAGATCCGATAAAAATAGAGGGAAGAGTGATAACGTCCCCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTAGATAACTATTATTTCACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAACAGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCTTCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACASI852DN854KV856LT858RS860DK862NR864GK866SD868NV870PS872EE874VV876KK878MK880NY882WR884QL886LN888AK890LI892TQ894RK896FD898NL900TK902AE904RG906GL9087251-11391SpCas99,8209,8409,8609,8809,9009,9209,9409,960
    CTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTGTTGAGACACGCCAGATCACCAAGCACGTGGCCCAAATTCTCGATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGGACTCAACCTATTTCGGCCGAAGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGCTAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAATAATGAGACTTCAGATTCGACCAGAGTCTAAAGTCNaeIPmlIPsrIBstXINgoMIVSE910LD912KA914GF916IK918RQ920LV922ET924RQ926IT928KH930VA932QI934LD936SR938MN940TK942YD944EN946DK948LI950RE952VK954VI956TL958KS960KL962VS964DF966R7251-11391SpCas99,98010,00010,02010,04010,06010,08010,10010,12010,140
    AAAGGACTTTCAGTTTTATAAGGTGAGAGAGATCAACAATTACCACCATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTGCACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTTAGGAAAATGATCGCAAAGTCTTTTCCTGAAAGTCAAAATATTCCACTCTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGACGTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACATGCTACAATCCTTTTACTAGCGTTTCAGABsgIFspAIK968DF970QF972YK974VR976EI978NN980YH982HA984HD986AY988LN990AV992VG994TA996LI998KK1000YP1002KL1004ES1006EF1008VY1010GD1012YK1014VY1016DV1018RK1020MI1022AK1024S7251-11391SpCas910,16010,18010,20010,22010,24010,26010,28010,30010,320
    GAGCAGGAAATAGGCAAGGCCACCGCTAAGTACTTCTTTTACAGCAATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAGAGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAGGGTAGGGATTTCGCGACAGTCCCTCGTCCTTTATCCGTTCCGGTGGCGATTCATGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTCTCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGTTCCCATCCCTAAAGCGCTGTCAGGNruIE1026QE1028IG1030KA1032TA1034KY1036FF1038YS1040NI1042MN1044FF1046KT1048EI1050TL1052AN1054GE1056IR1058KR1060PL1062IE1064TN1066GE1068TG1070EI1072VW1074DK1076GR1078DF1080AT1082V7251-11391SpCas910,34010,36010,38010,40010,42010,44010,46010,48010,500
    GGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTAAAAAGACCGAAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATTCTCCTACAGTCGCTTACAGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAATTTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGTCGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAAGAGGATGTCAGCGAATGTCKflIPaqCIR1084KV1086LS1088MP1090QV1092NI1094VK1096KT1098EV1100QT1102GG1104FS1106KE1108SI1110LP1112KR1114NS1116DK1118LI1120AR1122KK1124DW1126DP1128KK1130YG1132GF1134DS1136PT1138VA1140YS11427251-11391SpCas910,52010,54010,56010,58010,60010,62010,64010,660
    TGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGTCTAAAAAACTCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATCATTAAGCTTCCCAAGACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTTTTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTTTTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGTAATTCGAAGGGTTCTliIXhoIPaeR7IVL1144VV1146AK1148VE1150KG1152KS1154KK1156LK1158SV1160KE1162LL1164GI1166TI1168ME1170RS1172SF1174EK1176NP1178ID1180FL1182EA1184KG1186YK1188EV1190KK1192DL1194II1196KL1198PK12007251-11391SpCas910,68010,70010,72010,74010,76010,78010,80010,82010,840
    TACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACGAATGCTCGCTAGTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGCAGCTGTTCGATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGAGCGATCACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGAACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCGTCGACAAGCYS1202LF1204EL1206EN1208GR1210KR1212ML1214AS1216AG1218EL1220QK1222GN1224EL1226AL1228PS1230KY1232VN1234FL1236YL1238AS1240HY1242EK1244LK1246GS1248PE1250DN1252EQ1254KQ1256LF12587251-11391SpCas910,86010,88010,90010,92010,94010,96010,98011,00011,020
    TGGAACAACACAAACACTACCTTGATGAGATCATCGAGCAAATAAGCGAATTCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGACCAAACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTTTATTCGCTTAAGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTATTCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACTGGTTVE1260QH1262KH1264YL1266DE1268II1270EQ1272IS1274EF1276SK1278RV1280IL1282AD1284AN1286LD1288KV1290LS1292AY1294NK1296HR1298DK1300PI1302RE1304QA1306EN1308II1310HL1312FT1314LT1316N7251-11391SpCas911,04011,06011,08011,10011,12011,14011,16011,18011,200
    CTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGACACCACCATAGACAGAAAGCGGTACACCTCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACCCCAAGAAGGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCATGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGATACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCAscIL1318GA1320PA1322AF1324KY1326FD1328TT1330ID1332RK1334RY1336TS1338TK1340EV1342LD1344AT1346LI1348HQ1350SI1352TG1354LY1356ET1358RI1360DL1362SQ1364LG1366GD1368SR1370AD1372PK1374K7251-11391SpCas911,22011,24011,26011,28011,30011,32011,34011,360
    AAGAGGAAGGTGTGATCTCTTCTCGAGTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCATTCTCCTTCCACACTAGAGAAGAGCTCAGTACATTAATCAATACAGTGCGAATGTAAGTGCGGGAGGGGGGTGTAGGCGAGATTGGCTTTTCCTTCCTCAATCTGTTGGACTTCAGATCCAGGGATAAATAAAAAAATATCAATACAATCATAATTCTTGCAATAAATATAAAGTTliIXhoIPaeR7IK1376RK1378V*13807251-11391CYC1_terminatorSV4...LSNLS11,38011,40011,42011,44011,46011,48011,50011,52011,540
    AATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCGGCCGGTACccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactTTAAAAAGAAAAAAAAGACATGTCTGCGCACATGCGTACATTGTAATATGACTTTTGGAACGAACTCTTCCAAAACCCTGCGAGCTTCCGAAATTAAACGCCGGCCATGggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgttgcagcactgaKpnIMluIBsrGIAcc65ICYC1_terminatorT7T7_promoterM13-fwd11,56011,58011,60011,62011,64011,66011,68011,70011,720
    gggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggtcccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcgctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaBglILacZ alphaM13 originF1 ori11,74011,76011,78011,80011,82011,84011,86011,88011,900
    tacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcNaeINgoMIVM13 originF1 ori11,92011,94011,96011,98012,00012,02012,04012,060
    ggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattAloIM13 originF1 ori12,08012,10012,12012,14012,16012,18012,20012,22012,240
    aaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcctgatgcggtattttctccttacgcatctgtgcggttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaggactacgccataaaagaggaatgcgtagacacgccM13 origin12,26012,28012,30012,32012,340
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12349 bp90001000011000100020003000SpCas97251-113912272-3366Ashbya gossypii TEF1 promoter,BmgBI,MluI,NcoI,ZraI,AatII,PshAI,BstAPINcoIHygMX,hphMX open reading frame (ORF),AsiSI,RsrII,NdeI,BstAPI,SacIIAshbya gossypii TEF1 terminator,PacIHpaI,BaeILEU2 promoterBstEII,BspDI,ClaIAcc65I,AgeI,KpnI,PsrILEU2 open reading frame (ORF),XcmIBstXI,EcoRVBcgIKflIPciI,BmgBINgoMIV,NaeI,BstXI,PmlI,PsrIFspAI,BsgINruI,PaqCIKflIPaeR7I,TliI,XhoISV40 NLS,NLS,CYC1_terminator,AscI,PaeR7I,TliI,XhoI,BsrGI,MluIT7,T7_promoter,M13-fwd,LacZ alpha,M13 origin,F1 ori,Acc65I,KpnI,BglI,NgoMIV,NaeI,+1

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    12349